Document nmQDyJXGqMbJvK8wQa0mGQGX8

V l i '9 <Mg) STAIUSMCD 10DJ E. d u P o n t d e N e m o u r s & C o m p a n y IN CORPORATED W ilmington, Delaware 19898 POLYMER PRODUCTS DEPARTMENT e x p e r im e n t a l s t a t io n PERSONA! AND CONFIDENTIAL cc: A. J. Dahl B. W. Karrh L. J. Papa Pral File - 353 - NI 1400 - 269 y \ ' _; A*l2X(a- 1570 May 14, 1981 TO: DR. Y. L- POWER - PPD, Washington Works FROM: S. S. STAFFORD S - C t j ANALYSIS OF BLOOD SAMPLES FOR PERFLUOROOCTANOATE (Job No. 810-190; PRAL Nos. 81-1420-81-1467; Notebook Nos. E22514.E26238) As requested in your letter of 4/8/81 to L. J. Papa, the 48 blood samples submitted then have been analyzed for perfluorooctanoate (Cg). Results and sample Identification are given in the attached table. As noted there, the analysis was done using a gas chromatographic method specific for Cg (Lah Method Number ES-567) but results have been reported as ppm F for comparison with total organic fluorine analyses. Precision is 10% relative standard deviation over most of the concentration range, somewhat less at the lowest values. The lower limit for quantitation is 0.007 ppm F (0.01 ppm perfluorcoctanoic acid), with a detection limit of 0.004 ppm which can be distinguished from the reagent background but not well quantitated. Please contact me (772-4440) or L. J. Papa (772-2745) if you have any questions regarding the analyses. General questions on blood sampling can be directed to J. W. Raines or L. F. Percival. Attachment jah Keywords: Perfluorooctanoic Acid Perfluorooctanoate Blood Analysis GC There's a w o rld of things we're doing som ething about U000fe4 EXP000003 E ID 713817 TABLE I CONCENTRATION OF PERFLUOROOCTANOATE IN BLOOD (a) Sample PRAL Wo- 81-1420 Date Sampled 4/1/81 81-1421 81-1422 81-1423 81-1424 81-1425 81-1426 81-1427 81-1428 81-1429 81-1430 81-1431 81-1432 81-1433 81-1434 81-2435 81-1436 81-1437 81-1438 81-1439 81-1440 81-1441 81-1442 81-1443 81-1444 81-1445 81-1446 81-1447 81-1448 81-1449 81-1450 81-1451 4/1/81 4/2/81 4/2/81 4/3/81 4/3/81 4/6/81 4/6/81 4/6/81 4/6/81 4/6/81 4/6/81 4/6/81 4/6/81 4/6/81 4/6/81 4/6/81 4/6/81 4/6/81 4/6/81 4/6/81 4/6/81 4/6/81 4/6/81 4/6/81 4/6/81 4/6/81 4/6/81 4/6/81 4/6/81 4/6/81 4/6/81 P.R.No. 4443 4101 4226 4449 4530 4621 4490 4283 "WS WS 4109 4316 4282 4243 4520 4177 WS 4293 WS 4225 4182 4250 4547 4181 4551 4514 4624 4444 4251 WS 4111 WS Name GC Analysis Date Analyzed /11/81 4/15/81 [Cg], yg P/g l>loo<ifc 0.078 0.074 1.5 0.013 0.048 0.62 0.13 0.072 0.051 0.11 0.061 0.19 1 .0 5.1 0.44 0.052 0.23 0.11 0.17 0.31 0.054 0.077 0.31 4.3 0.64 1.30.14 0.57 0.18 0.15 0.83 3.8 0.22 (c) REDACTED* EXP000004 EID713818 000065  J v ? TABLE I (CONT"D) CONCENTRATION OF PERFLUOROOC1ANOATE IN BLOOD (a) Sample PRAL NO. 81-1452 81-1453 81-1454 81-1455 81-1456 81-1457 81-1458 81-1459 81-1460 81-1461 81-1462 81-1463 81-1464 81-1465 81-1466 81-1467 Date Sampled 4/6/81 4/6/81 4/6/81 4/6/81 4/6/81 4/6/81 4/7/81 4/7/81 4/7/81 4/7/81 4/7/81 4/7/81 4/7/81 4/7/81 4/7/81 4/7/81 P.R.l 4650 4448 4409 4400 4631 4234 4172 4382 4518 4299 4249 4300 4290 WS 4364 WS Name CC Analysis_________ ___________ . Date Analyzed [C^,], pg F/g blood 4/16/81 0.019 4/18/81 0.11 4/18/81 0.14 4/18/81 2.1 4/18/81 0.19 4/18/81 4.3 4/20/81 4.5 4/20/81 0.81 4/23/81 1.7 4/20 & 4/24/81 4.5 4/20/81 1.9 4/23/81 2.4 4/20/81 0.10 4/20/81 0.47 4/24/81 3.6 4/20/81 0.092 (a) Analysis as described in L; Acid in Blood, Gas Chromatographic Method", S. Stafford, 4/3/81), using the packed column GC analysis with perfluoro-n-octanoic acid as calibration standard. (b) Although the analysis is specifically for perfluorooctanoate (acid or salts), concentrations are given in ppm fluorine for comparison with the results of total organic fluorine analyses. (ppm F = 0.688 x ppm perfluorooctanoic acid) Estimated uncertainty is + 10% relative standard deviation. The lower limit for quantitation is 0.007 pgF/g. The detection limit is 'v 0.004 WgF/g, but concentrations in that range cannot be well quantitated and are reported as < 0.007. "None detected" is reported for samples with [Cg] < 0.004 ppm, which cannot be distinguished from reagent background. (c) In GC analysis of this sample one unusual large peak was observed in the region of interest, but no interference with the Cg peak was apparent. *REDACTED* EXPO00005 EID713819 000066 Z-4& "EV t* T (PDH) ESIAtUSMf D li E. I. d u P o n t d e N e m o u r s 51 C o m p a n y W , DINCORPORATED i l m i n g t o n e l a w a r e 19898 POLYMER PRODUCTS DEPARTMENT EXPERIMENTAL STATION PERSONAL AND CONFIDENTIAL cc: A. J. Dahl B. W. Karrh L. J. Papa Pral File I.C. - 353 - N11400 - 269 ^224-/57/ May 14, 1981 DCDR&. PW. E . NEELD CHAMBERS WORKS ANALYSIS OF BLOOD SAMPLES FOR PERFLUOROOCTANOATE (Job No. 810-578; PRAL No. 81-1554; Notebook Nos. E22514, E26238) As requested in your letter of 4/14/81, the blood sample submitted then has been analyzed for perfluorooctanoate (Cg) . The result is as follows. Sample Identification 4/13/81, age 24 GC Analysis* [Cg] = 0.26 Ug F/g blood (ppm) * Analysis as described in Lab Method ES-567 ("Determination of Perfluorooctanoic Acid in Blood, Gas Chromatographic Method", S. Stafford, 4/3/81), using the packed column GC analysis with perfluoro-n-octanoic acid calibration standard. Although the GC analysis is specifically for perfluorooctanoate (acid or salts), the concentration is given in ppm F for comparison with results of total fluorine analyses used earlier. (ppm F = 0.688 x ppm perfluorooctanoic acid) The estimated uncertainty is + 10% relative standard deviation, with a lower limit for quantitation of 0.007 ppm. Please contact me (772-4440) or L. J. Papa (772-2745) if you have any questions regarding the analysis. General questions on blood sampling can be directed to J. W. Raines or L. F. Percival. . Stafford jah Keywords: Perfluorooctanoic Acid Perfluorooctanoate Blood Analysis GC There's a w orld of things we re doing som ething about EXP000006 REDACTED* EID713820 000067