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AR226-3071 DuPontHL-1997-00261 HASKELL LABORATORY FOR TOXICOLOGY AND INDUSTRIAL MEDICINE STATIC, ACUTE 48-HOUR SCREENING TEST OF H-22274 TO Eafi&Bto SHIES PURPOSE: This is a rangefinding study designedto provide an estimate of aquatic toxicity using fixed test concentrations and data measurements at predetermined intervals. GENERAL INFORMATION MR: HASKELL NUMBER: TEST CODE: MATERIAL TESTED: SYNONYMS: SPONSOR: NOTEBOOK ID: SUBMITTER'S NOTEBOOK NUMBER: COMPOSITION: CONTAMINANTS: OTHER CODES: CAS REGISTRY: PURITY: TEST CONDITIONS Nominal concentrations tested: 0.0.5.1.0,50,500. and 5000 mg/L. Nominal Concentration (mg/L) At Test Initiation, O Hours Dissolved Oxygen pH (mg/L) A B A B At Test Completion, 48 Hours Dissolved Oxygen pH (mg/L) A B A B 0 03 1.0 50 500 5000 8.9 8.9 8.9 8.9 8.9 8.9 8.9 8.9 8.9 8.9 8.8 8.8 8.8 8.8 8.8 8.8 8.8 8.8 8.8 8.8 8.8 8.8 8.7 8.7 9.0 9.0 9.0 9.0 9.0 9.0 9.0 9.0 8.9 8.9 8.5' 8.5' 8.3 8.3 8.3 8.3 8.3 8.3 8.3 8.3 8.2 8.2 8.0* 8.01 ' Reading taken at 24 hours after exposure, due to total immobility. Page 1 of 2 _\. notcOTta'nTSW"" CoW^S.^-00" DuPontHL-1997-00261 HASKELL LABORATORY FOR TOXICOLOGY AND INDUSTRIAL MEDICINE STATIC, ACUTE 48-HOUR SCREENING TEST OF H.22274 TO Drob"^ FTE*"^ CONTINUED RESULTS Based on visual observations, the water control solution was clear and had no color. The 0.5,1.0, and 50 mg/L test solutions were clear and had no color. The 500 and 5000 mg/L test solutions were cloudy, white in color. Inunobilities were 0.0,0,0,40, and 100%, respectively. The 48-hour EC50, based on nominal concentrations and immobility, was 500 and 5000 mg/L. All water quality parameters were within acceptable limits. CONCLUSIONS H-22274 exhibited low toxicity in a 48-hour, unacrated. static acute test using Dachma fflagna. WORK BY: ^AA/ ^ Month/DDaay/Year AUTHORED BY: APPROVED BY: ^IW a (/ L'&.i ci hH. ^L-wLwty nDehbnonr>ah IL.. nDonbhrzryznsvldnfiki-^ Toxicology Associate ^ / ^ . MicbcUeR.Br" Study Director ^311^ Month/Day/Ycar J ^ ^ Month/Day/Ye DISTRIBUTION: Page2 Company Sanded. Does net conta-,n T^A C^