Document nmDJgBgnX4zpzakoJ14VaX4K2

AR 226 -- O765 NITRIFICATION INHIBITION IESTSuBSTANCE Identity: MriefxetrurreedctoontaasinPiFnOgSp,erFfCl-u9o5r,ooocrtaansesaulcfoomnaptoen,ewnhtiocfhFmCa-y20a3lsCoF-bXe or FC-203CF. (1-Octanesulfonic acid) (CAS # 2795-39-3). Remarks: 203CF-X. The 3M production lot number Current information indicates itis was 501. a mixture The test sample is of 1% PFOS, 20% FC- dsioedtihuymleoncetygllyscuollfamtoe,no1b%uttyrlieetthhaenro,l7am0i.n2e%, 2wa%tesru,l2fo.n7a5t%edAcarlykyllicetfhoearmesral,ts3,%and 0.05% tolyltriazole. cThhaerafcotlelrowiiznegd csounmcmenatrryataipopnlsieosftiompaumriitxiteusr.e Dwaittha imnacyomnpoltetaeclcyurately reflect inhibition by the fluorochemical component of the test sample. METHOD Method: ISOIDIS 9509 ISO Nitrification Inhibition Test Test type: Microbial inhibition GLP: No YAneaalrytCiocmapllmeotneidt:ori1n9g9:5 Oxidation of ammonia, pH and temperature. Statistical methods: Not given. Test organism source: Activated sludge mixed liquor collected from the pMreitorrotoWaussteeiwnattheerteTsrte).atment Plant, St. Paul, MN (Prewashed and adjusted Test condition: Dilution water: Millipore Mill-QTM water Mineral medium: Per method. Reference and test solution preparation: A stock solutionofthe reference substance, 1-allyl-2-thiourea, was prepared by dissolving = 116 mg in 100 mL of Mili-QTM water. Test substance was added Tdiersetctvlyestsoetless:t v5es0s0elmsL. glassErlenmeyer flasks with a total test volume Nofu2m5b0emrLo.f concentrations: four plus 1 reference substance and blank control TToetmaplersautsupreen:de1d9.s8o--li2d0s.6andC pH on day of testing: Initial TSS 4.98 _ ~ g/L which 1.49 g/L. was adjusted Initial pH 7.9. to 2.97 Blank g/L. TSS in the final control at 0-hr. pH 8.1 test solution andat 4-tr. was 8.5. Element Basis: Reduction in niification. 001286 RESOTS Nominal concentrations: Blank control, 82, 118, 252, and 496 mg/L Element values: 4-hour ECso = >496 mg/L Element values based on nominal concentrations. Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCluUsions The test substance 4-hour ECs for ISO Nitrification Inhibition test was >496 mglL, the highest concentration tested. This concentration did not have an impact on nitrifying organisms from activated sludge. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DpATAQUALTY Reliability: Klimisch ranking 2. Testing meets all criteria for quality testing. However, the sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. REFERENCES This study was conducted by the 3M Environmental Laboratory, St Paul, MN, Lab Request number N2977, 1995. O0 THER OOOOOOOOO Last changed: 6/28/00 001287 : BEST COPY AVAILABLE ENVIRONMENTAL LABORATORY ACTIVATEDSLUDGE MXEDLIQUOR ype Tost Lacs Activalod Slucdae AisriCiaition Dirh bili owAuRnaMSt2r95 2i7 7 Page I of 4 Te)ils 5c ACTIVATED SLUDGE MIXEDLIQUOR one Obtained 2[21[ 5 - oat uses 8/28/15 Miced Liquor Suspended Solids (MLSS)_Z-9Q on Adjusted 10 2.97 on Mss In final test soon _/- 79 ot tial pH ZL (1st test soln. prepared) Final pH ZL Gast sin. propared) Comments ESTSUBSTANCE Sample Description FC 203CF A nial pH of Nghest conc. tested (194m) = Bi Comments _ Adjusted to pH BEFERENCESUBSTANCE Sample Description = ; 2.1} Inidal pH of stock solution (11 & _ moi) = 4 Comments AId$ei,ch,LoRredo3Laulle3fnm Adjusted to pH M.A EXPOSURE CONDITIONS Test ovation A= tiga is, un the clu I Test Tomperature Range: 11.=4 20.1 C A 0012! revengere TOO fidnanl Shelae Nita Gatien Tri bien by, SampleDescription EnviroLnabm--eWonrktSaheelt o"n%e 8R.o34t19A50 Jadot soa Sd sol Test Selotiens Pepuralien RFeiebnaer3 - co 1M eam mm lanthovsen m[e0m6 JS e; Zacm ks Lombmf L1be mgm e ee Tsselved 116 mg sn. 10m Dll =Gost en eo Wereral 77 Tru yts baLs Mn L 2HIS gm RaS yJes inshed pi2aAL. 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STRTRS]] mon vosr 0oTnRSOr || mwesn RSme woe || mwTn TRSTame wc || mea RwoETs ; 1g le or sim av owlln seed f mazar stad senlod s eun) I Lo 8) awdlii) ge PN 2.7 de 001290 BEST COPY AVAILABLE 150 ACTIVATED SLUDGE NITRIFCATION INHIBITION TEST 150/DI5 9509 CALCULATION OF RESULTS M37 owe_2/a4l9s AuyPssT ASoAB EXPOSURE PERIOD TEoSneT. 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