Document nkoXOvG6jaLo9Mk30MRKg9636

AR226-2976 Study Trtte STATIC, ACUTE, 48^0U R #Q 5Q OF H -t9,520 TO Daptmiamagna Data Requirement Organisation fo r Economic Co-Operation and Development (OECD) Guideline for Testing Chemicals: 202 Author Keith B. Pierson, Ph.D. Study C pm pteM J2n July 10,1992 Performing Laboratory E. I. du Pont de Nemours and Company Haskell Laboratory for Toxicology and Industrial Medicine Elkton Road, Post Office Box 50 Newark, Delaware 19714 Laboratory Protect ID Haskell Laboratory Report No. 437-92 Page 1 of 19 " jnpany Sanitized. Does not contain TSC GBi Du Pont HLR 437-92 THIS PAGE RESERVED FOR SPECIFIC COUNTRY REQUIREMENTS j) 2 CompanySanitized. Does not contain TSCA CBS SottspanxSanitized- Does not contain TSCA O il A. J e s t.. .. S. Diiiition c. a le s t * * ' . S ' ...* ...... itc e e i ' I ( . .'.ft . . ...JS ...10 ...10 tV. Results and D iscussion.... a ft . .10 V. C onclusion....................... 1 * * * ' . ' . . . f t . * * . 11 VL Literature Cited. ..ft., ftCftftftftftftftft^f Vil. Tables 1. ChSmlcaiVCbaFacieristlcs of H a s itiii i a 2. rPWnkyaiistiieiiAcr aChiihi,em ist# e l the ^D-il'ution 1 4. Observed Ir -- ... 12 jW eli w a te r______ ... ..>...12 ft* ** . 1-3 T ea Solutions.. ......14 Ito H *19,52f fo r ._ ............................................................ .......................... o. h*1Stf20 Coheeniratiohs Causing1nfo?oiii##50% {EC5D) of D^pftnfemjigwaat SpecificTinfofoteivSiis........................... ...*17 VIM. Appendix .ft...,M*ftft..t.,.,..,,ft,,,,,0.,,.,#,,.,t,, ,,, t^,f,iMI,Miit.iitrti;i;Wv.itMHt(i(....... I. Protocol Amendments, SOP Deviations and Prdtoco! Deviations.. ......... 18 ....... 18 4 fismpanjr Sanitized. Does not contain TSCA CBt . ! tia ^ s lS M m te EuMte M istG ? Q m tm m m in; wiF*Afio&-n5cr^iSagoirrgyai w&9wiamwkAwSi.> i 5 Ceiipany Sanitized. Does notcontain TSCA CW ( } 6 figmgany Sanitized. Does not contain TSCA CBl 5 Rsaffi ToxlOlogist an# Study Director Environmental Science? M o/D aproar 7 JG&tnpany Sanitized, Does not contain T<ft g$$: t' Study D irector-6/25/92 Management - 7/19/92 Reported i'/lL Quality Assurance Auditor -}jja j9 ^ . Company amifeed,, Does noi confa* TSCA C W _T a . ': 4 - / ' . *vrv Du Pont H LR 437-92 '* ' '. .' I. p u r p o s e The goal of th is studyw as toassess theacute toxicity of H-19,520 to Daphnia magna IL RECORDS A il data and the fina! report m il be m aintainedeither at Haskell Laboratory for Toxicology and Industrial Medicine (Newark, Delaware) o r be archived at the Du Pont Records Management Center (W ilmington, Delaware). III. MATERIALS AND METHODS A . T e st S ub sta n ce The te st substance is described under General Information. A stock solution (1100 mL) was prepared by weighing the approp riate quantity of H-19,520, adding it to dilution w ater (see below) and stirring fo r 30 minutes. Individual test solutions (262 mL per flask) were then prepared from this stock in dilution water. Each of the above solutions was kept in a sealed container with minimal head space to avoid loss of the test substance through volatility. The stability and solubility of H-19,520 in dilution water (see below) and under storage conditions were not determined. B . D ilu tio n W ater W ater (Table 1) originated from the Haskell Laboratory well and flowed through aquaria containing fathead minnows, Pimephales promelas, prior to use in this daphnid test. C . A n im a l C u ltu re Parent Daphnia magna were reared at Haskell Laboratory in 250 mL Pyrex beakers (1 per beaker) which contained 200 mL of filtered dilution water or autoclaved fi. ered dilution w ater held at approximately 20C. Daphnids were fed two algal species, Anklstrodesm us fateatus and SelftOSSjaim capiiGflCTlUtom, at a rate of 75,000 ce!!s/mL of each species three tim es weekly during the water renewal process. Neonates (less than 24 hours old) were collected from i 4 day old parent daphnids for use In this test. Sickness, injury, and abnorm ality were not observed. Daphnia magna were identified by iabeis on the beakers and test vessels. D . T est M ethods [1] E ioht test concentrations and a water control were used. Nominal test concentrations were 7.82,15.6, 31.3, 62.5,125,251, 500 and 1000 mg/L. 9 Company Sanitized. Does not contain TSCA CBI *and dissolved oxygehprobes ... . . ......... ................. Total a.lk..a..l.i.n.ity (N ae"hkit, Nach Chemical Company, is la n d ; CD), iO m her<3o:^i(M ech kit, Hach Chemical Company. Loveland, CO), and COhdoetiv% |f $ l model ^3. Yellow Springs Instrum ents, Yellow Springs, ON) of the dilution w aterwere measured before daptm kis were added at the beginning of the test. X continuously-reOGiding therm om eter was used in a dummy control to Chech for temperature variation during the 48-hour test. E. Chemical Analysis Concentrations o f H -t9,520 in test solutions were not measured. F. Statistical Analysis The 24- and 48-hour 6050$ and their associated 95% fiducial lim its were calculated by the probit method using the logarithm of the concentration [2J. IV. RESULTS AN DISCUSSION A 48-hour rangefinding study was conducted in uncovered test chambers. Test concentrations were 0 .1 .1 .1 0 .1 0 0 , and 1,000 mg/L and a water control; observed im m obilities were 0 .0 ,0 ,0,100, and 0%, respectively. M obile daphnids in the 100 mg/L concentration appeared sluggish. The definitive study was conducted using nominal concentrations of 7.82,15.8, 31.3, 62.5,125, 251,500, and 1,000 mg/L. The test chambers were sealed to prevent loss 10 ompany Sanitized. Does not contain TSCA CBi I  I " ess Uian 24 h o u sed ,,inan V I. UTER TURE CITED ' ' % ^ K 4 | COnS iC? ^ aiCi (OECD). q * * . * 2` S I M SAS User's Guide: S ta lin , Verston 5 E(ion. 11 'Company Sanitized. Does not contain TSCA CBf {-' .`-nHrW t -?Da Pont H LR 437-92 STATIC-. ACUTE, H -1 9 ,5 2 0 f OF : T S tl^ '-. '. -' % i W . : , r : - .. . Chemical Characteristics of Hasfcel! Laboratory W eil W ater1 Param eter Concentration P aram eter BOD, mg/L COD, mg/L D O C ,m g/L TOC?, mg/L Total tqekfehi N, mg/L Ammonia N, mg/L Turbidity, NTU Phenolics, m g/L C olor, Co/Pt S olids Total suspended, mg/L Alum inum , mg/L Antimony, mg/L Arsenic, mg/L Beryllium , mg/L Cadmium, mg/L Calcium , mg/L C hloride3* mg/L Chromium, mg/L C obalt, mg/L Copper, mg/L Cyanide, m g/L iron3. mg/L F lu o rid e 3 <2 <10 2.8 <0.5 <0.2 <0.05 1.6 <0.050 <5 <4 <0.100 <0.060 <0.010 <0.001 <0.002 24.5 6.2 <0.010 <0.020 <0.010 <0.025 0.025 <o.1 --TT'-- mg/ L MBAS/LAS, mg/L Mercury, mg/L Nickel, mg/L N itrite3, mg/L Nieste3, mg/L Priosphat3, mg/L Potassium, mg/L Selenium, mg/L Stiver, mg/L Sodium, mg/L Sulfate3. mg/L Sulfide, mg/L Thallium , mg/L Zinc4, mg/L pollutants Acid extractable priority pollutants Base/neutral priority poiiutants Pesticides/PCBs iirganppnpspnan pesticides3, pg/L C on centration <0.075 3.8 <0.05 < 0.00020 < 0.020 <0.1 2.1 <0.1 1.8 <0.005 <0.010 8.0 4.8 <0.05 <0.01 <0.020 ND3 NDS ND3 ND3 <0.50 fDate of sample collection 30 December 1991 unless indicated otherwise, analyses performed at Environmental Testing and C ertification Corporation, Edison, New Jersey spate of sample collection 15 January 1992, performed at Du Pont Engineering Test Center Newark, Delaware; 3Date of analyses 13 January 1992, analyses performed at Du Pont Chemicals, Jackson Laboratory, Deepwater, New Jersey; 4Below the Published Method Detection Um it (BMDL); Analyses performed at Hazleton Laboratories, Inc., Madison, Wi; 6None detected. 12 Company Sanitized. Does not contain TSCA Crfl 13 Company Sanitized, Does not contain TSCA GBI k/.- Dissolved O xvoenm p/L Mg Control 6.6 b 7.82 8,6 b 15.8 a * b 31.3 3.6 b 62.5 8.6 b 125 8.6 b 251 8.6 b 500 8.6 b 1000 8.7 b pH b5 bb bb bb bb bb bb bb bb 8.5 8.6 8.5 S J 8.6 8.6 8.5 8.6 8.6 8.5 8.4 8.4 8.4 8.5 6.6 8 J 8.5 8.5 8.5 8.4 8.5 8.5 6 4 8.5 8.6 8.5 8.5 8.5 8.5 8.4 8.4 8.5 8.4 8.4 8.5 8.4 H2O Control 7.3 b b b 7.82 7.3 b b h 15.6 7.3 b b b 31.3 7.3 b b b 62.5 7.3 b b b 125 7.4 b b b 251 7.4 b b b 500 7.4 b b b 1000 7.4 b b b 7.5 7.5 7.5 7.5 7.5 7.5 7.5 7.5 7.5 7.5 7.-5 7.5 7.5 7.5 7.5 7.5 7.5 7.5 7.5 7.5 7.5 7.1 7S 7M 7.5 7.5 7.5 7M 7.5 7.5 7.5 7.5 7.5 7.5 7.5 7.5 *A, B, C. and D represent replicate test vessels containing five daphnids each at test ^See last paragraph of D. Test Methods for explanation as to why a single reading was 14 Company Sanitized. Does not contain TSCA CBJ (mg/L) A i a Ga Da Aa Ba Ca Pa Temperature. *>G H2O Control 20.2 b b b 7.82 20.2 b b b 15.6 26.2 b b b 31.3 26.2 b b b 62.5 125 mb b b bbb 251 20.2 b b b 560 26.2 b b b 1606 20.2 b b b 19.9 19.9 19.8 19.8 19.9 19.8 19.9 19.9 19.9 19.9 19.8 19.8 19.8 19.9 19.8 19.9 19.9 19.9 19.9 19.8 19.8 19.9 19.8 19.8 19.9 19.9 19.9 19.9 19.8 19.6 19.9 19.6:19.8 19.9 19.9 19.9 aA, B, C, and D represent replicate test vessels containing five daphnids eac.v a tte st s ta rt ; bSss last paragraph of D. Test Methods for explanation as ic why a single reading *$ made. v' ' . i ir 15 Company Sanitized. Does not contain TSCA CBl Nom inal C o n te n tio n s (mg/L) A 24 Mom Ba Ca Da W ater Control 0 0 0 0 7.82 15.6 31.3 0 00 0 0 0 0 0 0 0 r, 0 62.5 0 00 0 125 0 0 0 0 251 40 20 0 0 500 20 40 40 20 1000 30 40 20 60 40 Moors Aa Ba Ca ? 0080 0 0 08 0 0 00 0 20 0 8 40 40 20 40 60 40 60 40 80 80 40 60 80 80 60 80 too 86 80 60 sta itA' B' anCl P fpresent repticat9 test vessels containing five daphnids each at fest i 't JS .5 ,i i } .5 /y 16 Company Sanitized, Does not contain TSCA CBl m b Pont HLR 437-92 PROTO! DP H -r9 ^0 T O D m n > am agnS APPENDIX I PROTOCOL AMENDMENT NUMBER ONE a. T h M e fii^ o n o f^ m o ^ tio n n page 8 f ^ 1 1 not fee used In Iteu thereof imm obilization w ill b e ^ e fin e g ^ th e k m fiffS E m fa least two body lengths m any one direction! within 15 se p o ys aftepgentfe prodding with a glass red. b. Daphnids w ill be distributed to test chambers using random numbers. No more than two daphnids w ill be placed into one chamber per random number. c A registered (not calibrated) American Society of Testing M aterials mercury therm om eter w ill be used to take temperatures of test solutions. d. Analytical measurements of test solution concentrations w ill not be made. e. Surviving daphnids at test end w ill be sacrificed according to S O I ^ m i f. Sdfubility and stability studies of the test substance in dilution water w ill not be perform ed. f t P ^ e four the sentence "a test is not acceptable if more than T0% of the daphnids die or show abnormal behavior in either control" is am ftguous. The sentence should read "a test is not acceptable if more than 10% of th e daphnids die o r show abnormal behavior in any of the four replicate controls". h. Beakers being acclimated prior to use in the definitive test w ill be covered by plastic-coated paper (plastic side dow n)-not glass as described in the SOP. i. Temperatures w ill be taken at the start of the test before addition of daphnids and at th e end o f the test after the final immobilization readings are made. Temperature variation w ill be assessed by a continuously-recording probe which w ill be placed in a dummy control vessel sitting adjacent to one of the four water controls in the water b a th . PROTOCOL AMENDMENT NUMBER TWO a. Based on the initial Range-finding test, the concentration levels (Test Substance) w ill be:7.82,15.6, 31.3, 62.5,125, 251, 500,1000 mg/L to increase the probability tha zero im m obilities w ill occur at the lowest test concentration. I8 Cempaity Sanitized. Doss not contain tsca CBl c. P r0-G 9nffiim irig J p ro to c o l taken was not documented in study records, not doeum entid Jn situdy records.