Document nknN41qyzVyjybGzYkD2Y6JO1

ACTIVATED SLUDGE RESPIRATION INHIBITION TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may also be referredtoas PFOS, FC-95, or as a component of FC-185F. (1Octanesulfonicacid)(CAS # 2795-39-3) Remarks: The 3M productionlotnumber was 1-2303. The testsample is FC-185F, cc 817-19. Currentinformationindicatesitisa mixtureof4% PFOS, 36% diethyleneglycolbutylether,29.29% water,14% ethyleneglycol, 8% Sultonefoamer,8.35% sodium octylsulfate0,.3% sodium laurylsulfate, and 0.06% tolyltriazole. The following summary applies toa mixture with incompletely characterized concentrations of impurities.Date may not accurately reflectthe toxicityof the fluoruchemical component of the testsample. METHOD: Methods: ProtocolReference EAR, 11/8f79. Test type: Aerobic. GLP: No Year completed: 1982 Analyticalmonitoring: Dissolvedoxygen concentrations Test organism source: Activatedsludgemixed liquorfromthe St. Paul/Minneapolis,Minnesota Metro Wastewater Plant Test conditions: Dilutionwater: Deionizedwater. Reference and testsolutionpreparation: A primarystocksolution was prepared by dilutin0g.60 g into100 mL deionizedwater. Test Vessels: Not given. Number of concentrations: 1 plusblank control Temperature: Not given. Total suspended solids and pH on day of testing: Not given. Element basis: Respirationinhibitioans determinedby oxygen consumption. RESULTS Nominal concentrations: Bk control,1000 rng/L Element values: No Inhibitioonfactivatedsludge respiratiornateat 1000 mg/L witha contacttime of 3 minutes. Element values based on nominal concentration. Remarks: Testinwgas conductedon themixturaes describeidntheTest Substance Remarks field.The values reportedapplytothatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS The testmaterialexhibitedno inhibitoreyffectsat 1000 mg/L over a 3 minute exposure period. Submifter: 3M Company, Environmental Laboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking= 3. Testinglacksrecordofmethodology used. The method was not an agency-approved method. There isa lackof characterizatioonfthe testsample. Test solutionswere not analyzedfor PFOS concentration. REFERENCES This study was conducted by the 3M Company, EnvironmentalLaboratory, St.Paul,MN, Lab Request number 7544. OTHER Last changed: 6/26/00 8 < ox a1 Za w IL -T- r 7 -4- ENVIRONMENTAL LABORATORY SOMMRY SHEET TEST: Acute Toxicity of a Compound to Activated Sludge Mixed Liquor. SAMPLE DESCR IPT.: FC BSt-ANALYST: WADE -A,Sc kc-I(-, DATE ANALYZED: I- -2_g- S2_ Z303 LAB REQ. NO.: --- - - - --- --- -tfff F7T-T- IT 5 z LL) >c 4 --A L3 Z Z CL -------------- 0x c70Int 3 --------------- 2 ------- ELTT ------- --------- --- --------IT --------------------- ------- -------------------------- ------------- - ---------$-- --------- -H44@P ------ -- - - - - - - - - -- - - ------- - ------------ - ---------------------- ----------------- -------- - --- - -- ---- --- -- o@----------------------------- ---- -T-T- -----------------------7-7--7---------------------------- ----- ------------------------------------- 4 TIME minutes 3M Environmental Laboratory Acute Toxicity of a Compound (Substance) to.-ActivatedSludge Mixed Liquor Protc>col Reference: EAR 11/8/79 Lab request no.: Date: /-20- e2- Ana Iys t: The activated sludge mixed liquor was collected from the St. Paul/Minneapolis Metro Wastewater Plant on S(2- Sample description: Z-o Preparation of stock solution.- 0.(00 grams of sample were diluted to /0--o m I with deionized reagent water. Date of preparation: '"TlME min. sec. C05C NT@@ ION O@O;EST MA;ERIAL IN A I A E SL GE @I)IBRE I DO CBHTR mg/i mg/i ico.sto@ls, M4@? mi 15@ 13o t2c) 2-0 -2. 4f 90 n a COMMENTS: L L @4 1 4.t;j '