Document nkJXgQV2jG5eVo5x4pZoK65z6
- 070s
M ICROBIOS M ICRO TOX T O X IC IT Y T E S T
T E S T SU B STA N C E_________________________________________________
Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFO S, FC-95, or as a component of FC-2Q1F-X. (1Octanesulfonic acid) (C A S # 2795-39-3).
Rem arks: The 3M production lot number was 1. The test sample is F C 201 F -X (F -1 1129). Current information indicates it is a mixture 3% PFO S, 36% diethylene glycol butyl ether, 36.6% water, 8.25% acrylic foamer, 7% sodium octyl sulfate, 6% sulfonated alkyl ether salts, 3% triethanolamine, and 0.15% toluoltriazole.
The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect the toxicity o f the fiuorochemical component o f the test sample.
M ETHOD:__________________________ _______________________________
M ethod: Microbios Microtox Procedure T e st type: Acute static GLP: No Year Com pleted: 1992 Species: Photobacterium phosphoreum A nalytical m onitoring: pH and osmotic condition. Exposure period: 30-minutes Statistical m ethods: EC50 values calculated by statistical linear regression program provided for Microtox T e st organism so u rce : Microbics' Reagent. Te st conditions:
Diluent: Millipore Milli-QTM water T e st tem perature: Not noted. Stock and te st so lu tio n preparation: A primary stock solution was prepared by adding 200 mg of test material to 100 mL Millipore Milli-QTM water. The pH of the stock solution was 7.4. An osmotic adjustment was made using 200 mg NaCI dissolved in 10 mL stock solution. Appearance of test solutions was noted as "clear and colorless". All test solutions were made by proportional dilutions. Exposure ve sse ls: 4 mL cuvettes. Num ber o f replicates: two Num ber o f concentrations: Four plus a blank control Elem ent b a sis: Percent light loss
R ESU LTS____________________________
Nominal concentrations: Blank control, 125, 250, 500, and 1000 mg/L
000388
Elem ent value:
5-minute EC50 = 153 (130 - 180) mg/L
15-minute E C 50 = 135 (108 - 170) mg/L 30-minute E C 50 = 110 (84 - 145) mg/L
Element values based on nominal concentrations.
Rem arks: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone.
C O N C LU SIO N S ___________________________________________________
The test substance 30-minute E C 50 for Photobacterium phosphoneum was
determined to be 110 mg/L with a 95% confidence interval of 84 to 145 mg/L.
Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, MN, 55133
DATA Q U A LITY____________________________________________________
R eliability: Klimisch ranking 2. Testing meets all criteria for quality testing. However, sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution.
R EFER EN C ES ____________________________________________________
This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number K1126-1,1992.
O TH ER _______ ___________________________________________________
Last changed: 6/26/00.
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NUMBER
CONC.
GAMMA
G A M M A E S T . 7. E F F E C T
1
125.0000
0.7700
0.8583
43.503
Q
125.0000
0.7906
0.8583
44.152
3
250.0000
1.6893
1.5336
62.816
4
250.0000
1.7168
1.5336
63.191
5
500.0000
3.0036
2.7402
75.023
6
500.0000
2.9126
2.7402
74.442
7
1000.0000
4.6187
4.8964
82.202
a
1<1)0 0 .0 0 0 0
4.3718
4.8964
81.384
EC 50 = 152.6346 mg/L (957. C O N F I D E N C E RANGE: 1 2 9.7237 TO 179.5920)
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000391
mCROTOX DATA REPORT
F ILE NAME: K 1 126-1. K5 Sample Description:
TEST DATE: TEST TIME:
201F-X FI 1129 LOT 1 BROWN LIQUID TEST DATE 4/17/92
Procedure: BASIC Initial Concentration ; 1000 mg/L Test Time: 5 minutes
Osmotic Adjustment: NaC 1 Dilution Factor : 2
Concentration Units: mg/L
NUMBER IO/IT
CONC.
GAMMA
1 86/ 42
125.0000
2 87/ 42 125.0000
3 84/ 27 250.0000
4 88/ 28 250.0000
5 88/ 19 500.0000
6 86/ 19 5 0 0 . 0 0 0 0
7
91/ 14
1000.0000
8
87/ 14
1000.0000
0.77000# 0.79059# 1.68931# 1.71675# 3.00363# 2.91264# 4.61873# 4.37176#
CONTROL IT/10 = 80/ 94
BLANK. R A T I O = 0 . 8 6 4 4
10 ZBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBDBBBBBBBBBBBBBBBBBBBBBBBBD p
3SL0PE = 0.8374 7
3 5
33
63
83
A3
3
M3
63
M3
3
A3
4_
3
1 3> z z z z z z z z z z z z z z z z z z z z z z _ *
3 ' ____.
3
3<*
3 _ 2.
3
3
______
.
3
3
_______
.
3
3 __
.
3
33
______ _______
.' .'
3 3
3 ______
.EC 50
3
.10 G3 BBBBBBBBBBBBBBBBBBB'BBB.BBBBBBBBBBBBBBB-'BBBBBBBBBBBBBBBBBBBYj
10 100 CONCENTRATION 1000
10E4
EC 50 = 152.6346 <95% CONFIDENCE RANGE: 129.7237 TO 179.5920) _ # Used -for c a l c u l a t i o n
000392
^ of )0
STATISTICAL REPORT FOR DATA FILE K 1 126-1.K5
ESTIMATING EQUATION: LOG C = 1.1702 k LOG b +2.1837
NUMBER OF DATA PAIRS USED: S
RESIDUAL VARIANCE: O .00303
957. C O N F I D E N C E FACTOR: 1.17661 FOR EC50
CORRELATION COEFFICIENT: R = 0.98991
E F F E C T I V E C O N C E N T R A T I O N S (957. C O N F I D E N C E RANGE):
EC 50 EC 20
EC 10 EC 1
= 152.6346 mg/L = 30.1391 mg/L
= 11.6684 mg/L = 0.7053 mg/L
( 129.7237 TO ( 20.8449 TO
( 7.0781 TO ( 0.2880 TO
.179.5920) 43.5773)***
19.2355)*** 1.7272)***
*** EXTRAPOLATED RESULTS
0C0393
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( 5 1S S "69 T 01 TOS *8 0 1 ! 39l\iya 33N3C IId N 03 S 6`/. >
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8 3 S "5 T83S-S Z b 8 0 - Z b B O ' 3 T 3 Z *T 3 T 3 Z 'T b 0 9 6 *0 b 0 9 6 `0
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0 0 0 0 `000 T
0 0 0 0 *000 T
0 0 0 0 *005
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0 0 0 0 `0 53
0 0
000 000
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MICROTOX DATA REPORT
FILE NAME: K 1 126-1. K 15 Sample Description:
TEST DATE: TEST TIME:
201F-X FI 1129 LOT 1 BROWN LIQUID TEST DATE 4/17/92
Procedure: BASIC Initial Concentration : 1000 mg/L Test Time: 15 minutes
Osmotic Adjustment: NaCl Dilution Factor : 2
Concentration Units: mg/L
NUMBER 1 0 / IT
CONC.
GAMMA
1 86/ 38
125.0000
y,
87/ 40 84/ 24
125.0000 250.0000
4 88/ 25 250.0000
5 88/ 17 5 0 0 . 0 0 0 0
6 86/ 16 500.0000
7
91/ 12
1000.0000
a
87/ 13
1000.0000
089306# 0.82124# 1.93073# 1.94748# 3.33453# 3.50077# '5.34992# 4.60382#
CONTROL IT/10 = 77/ 94
BLANK RATIO = 0.8374
10
G A M M A
1
, 10
Z-333333333333333>3Q33DDzSDDzLDD0zBPzDEBzDzD=BzDDzDD0zD.DzD8DzD4Dz 1DDz 7DDz DDz DzDDzDDzDDz'BzD''zD_DD2D*..DB_E_DDCDDUD5D4D0DBDDDBDBDVDD6DDDD_DDDD__DDD^DD87BDDUVDDDDDDDDDDDDDDDDDDBDDUDDDDDDBDD3333?3333333333Y333<3
10
100 CONCENTRATION 1000
10E4
EC 5 0 = 1 3 5 .4463 (957. C O N F I D E N C E RANGE: 103.2013 TO 169.5515) # Used -for c a l c u l a t i o n
OC0395
I d - I'J
S T A T I S T I C A L R E P O R T F O R DATA F I L E K 1 126-1.1a 5
E S T I M A T I N G EQUATION: LOG C = 1.1494 ;< LOG b + 2 .1318
NUMBER OF DATA PAIRS USED: 8
RESIDUAL VARIANCE: 0.00492
95*/. C O N F I D E N C E FACTOR: 1.25180 F O R E C 5 0
CORRELATION COEFFICIENT: R = 0.98358
E F F E C T I V E C O N C E N T R A T I O N S (957. C O N F I D E N C E RANGE):
EC 50 = 135.4463 ma/L EC 20 = 27.5266 mg/L EC 10 = 10.8380 mg/L EC 1 = 0.6886 mg/L
( 108.2013 TO < 16.8924 TO ( 5.6365 TO ( 0.2175 TO
169.5515) 44.8552)*** 20.8398)*** 2.1798)***
*** EXTRAPOLATED RESULTS
000396
J
FINAL RESULTS
NUMBER
CONC.
1 125.0000 2 125.0000 3 250.0000 4 250.0000 5 500.0000 6 500.0000 7 1000.0000
8 1000.0000
EC 50 = 110'.3339 mg/L
3c> - m m
GAMMA
GAMMA EST.
1.0258 0.9939 2.3920 2.3920 3.9749 4.2091 6.0152 5.7068
1.1536 1.1536 2.0627 2.0627 3.6883 3.6833 6.5950 6.5950
(957. CONFIDENCE RANGE:
Kl 126-1.K30
7. EFFECT
50.636 49.848 70.519 70.519 79.899 80.803 85.745 85.090
83.8096 TO 145.2529)
H H H ilM H 11H H H 11H 1111M H M M H MM 11HH HMH MM MM Mil11M 111MH MH MM MM MM 11MH H 1111il11H 11H 1111MH H 111MM H H MM MitHflHMH C C C Touch SPACE BAR to continue... .C C C C C C E C C C i C C i i C C C C C C C C C C C C C C C C E C C C C C C C C C C i
000337
MICROTQX DATA REPORT
FILE NAME: K 1 126-1. K30 Sample Description:
TEST DATE: TEST TIME:
201F-X F I 1129 LOT 1 BROWN LIQUID TEST DATE 4/17/92
Procedure: BASIC
Initial Concentration : 1000 mg/L Test Time: 30 minutes
Osmotic Adjustment: NaCl Dilution Factor : 2
Concentration Units: ma/L
NUMBER 10/ IT
CONC.
GAMMA
1 86/ 36
125.0000
2y,
87/ 37 .84/ 21
125.0000 250.0000
4 88/ 22 250.0000
5 88/ 15 500.0000
6 86/ 14 500.0000
7
91/ 11
1000.0000
8
87/ 11
1000.0000
1.02576# 0.99392#
2.39196# 2.39196#
3.97488# 4.20908# 6.01519# 5.70683#
CONTROL IT/10 = 79/ 94
BLANK RATIO 0.8480
10 ZBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB?
3SL0PE = 0.8384
3
G A M M A
33333333
3333>33
z
z
zzzzzzzzzzzzzzzzz
___1 _______ ______ ________
_________ ________
1
2
8
6 5
3 3 3 3 3 3 3 3 <
3 3 3 3
3 3
3 _________
3
3 ______
EC 50
3
.10 G3 BBBBBBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBBB'-BBBBBBBBBBBBBBBBBBBY3
10
100 CONCENTRATION 1000
10E4
EC 50 = 110.3339 (957. C O N F I D E N C E RANGE: 8 3 . 8 0 9 6 TO 145.2529) # Used -for c a l c u l a t i o n s
00039S
1
STATISTICAL REPORT FOR DATA FILE K 1 126-1.K30
E S T I M A T I N G EQUATION: L OG C = 1.1478 :< LOG > + 2 . 0 4 2 7
NUMBER OF DATA PAIRS USED: 8
RESIDUAL VARIANCE: 0.00569
957. C O N F I D E N C E FACTOR: 1.31648 F OR EC50
CORRELATION COEFFICIENT: R = 0.98097
E F F E C T I V E C O N C E N T R A T I O N S <957. C O N F I D E N C E RANGE): .
E C -50 EC 20 EC 10 EC 1
= 110.3339 ma/L = 22.4741 mg/L = 8.8605 ma/L = 0.5652 mg/L
< 83.8096 TO < 12.7709 TO ( 4.2097 TO < 0.1568 TO
145.2529)*** -39.5497)***
18.6493)*** 2.0365)***
*** EXTRAPOLATED RESULTS
000399
