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APILOTREPRODUCTsIoOsN; STUDYWITHTHE MALLARD
FINALREPORT
WILDLIFE INTERNATIONAL, LTD PROJECT NUMBER: 434105
MLAB REQUESTNO.UZ3
FRA Guten714
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AUTHORS:RSaeTaronmmPo.BnGdealLvlaaVghanesrkoven
STUDYINTIATIONDATE: erry 28,200 STUDY COMPLETION DATE: December 15,2003
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GOOD LABORATORY PRACTICE COMPLIANCE STATEMENT
SPONSOR: 3MCorporation
TITLE:
PFOS: APilotReproStdudyuwictht theiMao llanrd
WILDLIFE INTERNATIONAL, LTD. PROJECT NUMBER: 454-105
STUDY COMPLETION: December 18,2003
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STUDYDIRECTOR:
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SPONSOR'SREPRESENTATIVE:
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GOOD LABORATORY PRACTICE COMPLIANCE STATEMENT
SPONSOR: 3M Corporation
TITLE:
'PFOS: APilotReproStdudyuwicthttheiMao llanrd
`WILDLIFE INTERNATIONAL, LTD, PROJECTNUMBER: 454-105
STUDY COMPLETION: December 18,2003
GboyothdLaUbTSoi.reastEotryymPwraavscicocinedPu(rcootEteedNncitVinomcInoAmMgpeelCniceInsy(C,949Hi0w)iEC1t7FahM)RG;.osPloadrdtLJe1ab6p0oa,rnsM1o7ArAyFuPFgr,uasc3ti9c1N9eo8S3tb;aSnOadEaaNCrDdosPtr3iSnpcciuapbllseisNodooenfd.
3850,AgriculturalProductionBureau, 10August 1984.
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STUDY DIRECTOR:
SmNGaelhgher Tiller
Sesor Biologist, Avi Toxicology
SPONSOR'SREPRESENTATIVE:
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: 0003589
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QUALITY ASSURANCE STATEMENT
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AcTvITY
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STUDY DIRECTOR:
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CHEMISTRY PRINCIPALINVESTIGATOR:
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MANAGEMENT:
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TAOPFBACGOE2NLTENETS
TABLES
Table 1. MeosrasMeslsareddCFolnocteRnteraptiroOnsQoUpSCmUO.R)ofrFFrOSirnAvianDiet
1
Table 2.MeanAdultBodyWeight 1) fomaMallard
Table 3, MPeiatnRFeeedCponrsumopStdiaonuy(c9wbiiihrdPoRdOySn),fowmeaMrallard
29
Table 4. SPuimomaRerepymroofd4GurcdotTsieosnPtSaTtthdRoylRowgRiitcBhalOPOFRbOsSer,vAardtuilortnsBfrirrodmsBbMaanlilazredd
30
Table 5.NMeianbEggBPiroodRuctieon(tEggosSLeahiydieWinloahnPdRnEOgSg.sHeaDayc)frrom c 3)
Table 6. SoummmaaryoalfaRredpFrioldoucRtivEePpeforrmOaSntc8ue(yuBWgigctshSPeaOtSfnr.om Weck )
"Table 7. MeoamnBoMdaylWredigPhilto(tgR)oefHpatcrhloindgsSatuunddcySuWtrivtiihvBoinFgOnOSffsprving.v
33
Tule 5.MeanLiverWeights 5) fromaMallard PotReproduction
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TAOPFB ACGOENSLTENETS
APPENDICES
Appendix TL. DickandSUpIGTED FOTDUIBONS ven: 3
Appendix IV. TheAnaOfl PFOy SinsAVi IDDs iel cummins40
Appendix V. DiaoFgTrtLaayomtt cvs 34
Appendix VL
Appendix VIL AppendixVILL
Appendix TX. Appendix X.
AdRulet PBodryWOeSiogdhytU(gW)EAfNrBooRmOnSaM.allardPirlevoeret
35
FPieleodtRCoenspumrptoiond(Sguuhdicyrdtwdaiiyh)PfoRoOnmS Mr allard s $9
I`MnadlilvairdduPaillortoRssPeatpholrogoica1dl2Ob6usewrcivtaithiPoonFsOfnSromv a rs 63
HisopathOOgY REPO.
61
MEgagPrrPilootR(dgeesupircdoSte1uudeiycw)iiohroPonFmOnaS. rcv 106
Appendix XL MReaptraordudctPiivoeRPeerfoprmranocebdSyPaueyncWfirttohmiaon POS. .
10
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Appendix XII MeOatnROfefspprirngoBodSdyKuWYeciwgihtht(iPg)RofOrSonm aMralrlarrd.
16
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Appendix XII ARduletLivperWreSihgaOhdtyW(d5H)frPUoRmOaSCM.alolardnPilot rere 11
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Appendix XIV. OfPiflsoptrRienpgrLoidvuectriWoeniSghtt(g)WfiutrhoPmdFSa.yM.a.llard
Appendix. XV. CHA0SNYPGIOOEGOL rrr Appendix XVI. Personnel1aVOWedin SU.
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WI iWillddn lliifft ee Ine terr natin onala, LLtt edd..i~ o~ nalProje, ct NumbW er 454E -105 RT a
SUMMARY
STUDY: PROS: APilot ReproSuddyuwicthttheiMaollanrd
SPONSOR: 3M Corporation
WILDLIFE INTERNATIONAL, LTD. PROJECTNUMBER: 454-105
TESTDATES: SENtxeupueyrTIinmekeinmnailioSn--oaFae-FbAterpbaeoylnn21a8,r&2y0J02l90,201004,2000 BiologicalTermination ly 25, 1000
TEST ANIMALS: Mallard (asplayin) AGETESTANIMALS: Appr27oweekxsofiag tmhantineofltheyest SOURCE TEST ANIMALS: W11h3iWtaisnhginWgiinogns,Stoece.t
HUaSnoAveIr, S1041.0509
`NOMINALTEST CONCENTRATIONS: 0,18,62, and 17. ppm ai.
RESULTS: MiaTlofrlosfweawaerkae.poToosneecdo1nt9otwoPelFgeOr.SouNapoadntidreeta1a7tr.ym6pconmecean1t.reateimsotongsrroaoflw0e,wse1wr,eerm6ea.2oibasnnedtrva1e7doi.6anpaeoeydsmt oF heconcenratiteostneds. Noovrsignsofancitywereotedatthe 8or6.2pra
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`ati.ieteisstocotencoeefntedrartiloinfs.thAttorntehbyeo1db7ya.vw6eebplgephemt asF.mie.oltntegessdttcoomnacleenatstraaentrdio,fne,Tmhaaeslrieensgwleerhheee1nw01.as8sp4npdoatr6ee.dt2
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pPrTpoenmieae.si.itsSnepsta7terc6eonn1pc7e.pn6etmprsapmtamiie.onans4s.ee.taa`etTmtehegedrrneeogfwufrepeocurttpseesmto.nmeTaafhynoebhdgoaecvdwoenyebswrueeemipmagt0ihrotaenpllopoiarsersdeetn3sota3prmtrrooeadantugmicemtnimato.lneeisTnnehatenhtrdede
tits on any ofto reproduciv paramerrs mpgs. Hstopathologica examination
oiofrsoenlvi eeecstceradlt etaisiisouoenfrofteovirwesaadlesuetldustadty,heitghshnecraointoacbhiad1er7n:n6vfcodefptoeffotdemestte1ci.scountGlracorccurpno,eagdrieWooshsntiilobfenertaphmnreadeslcelaladuoridbdpessodes.rcevxaBpttiaoisssoseenuddse
(SPFOS nthe ictfor sx kswas 6.2ppmai.
600364
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Wildlife International, Ltd.
ProjctNumber454-105
5. INTRODUCTION Thi study was conducted by Wilde Intemational, Ld. for 3M Corporation f the Widife Iotematioal, Li. avin toxicology aclinEaton, Maryland 21601. The iological portionofthe est was conducted rom February 29,200 Jy 25,2000 Rave dt geread Wilde eration, Ltd. and copyofthefoal reportarefledunderProjoctNumber454105inarchiveslocatedonthe Wii Inermationl, Lid. it. Biological specimens a store st 3M Corporation, 1. Paul, Minoesota ssi.
omECTIVES heobjectiveofistdywas evaluatethefts pontheadultMalad(dnaplayonehos) ofdietaryexposure thepotassiumsaltofPerluorooctneSuforicAck(hearrefered o28 PFOS) ocveor napseudwmoerfpeatevpailupoaternd.oInx6adwideietmikosnao,rth1e9eewffelecetksysof EaflfetctesxpoosaurdeittohPeRaOlS,obnodthyewneuimhbi,ernodfef3eo5d3 1d, fer, embryo viability, hachabily and offspring survival were evaluated. Hitopubologieal ccaminationofseesedtusananalysesofbloodandtsuesamplewere alsousd 10 eval the lfcuponadultsexpose oPFOSaad totei ofpring
EXPERIMENTAL DESIGN
rstmeManltlgarrodups(2.0Tmhaelteessat cdonc2en0tfreamtailonessw)weerreeralncdiodmilnycdoinsisbuultteda iwtiothotnhe Scpoontnrsoolr,grboauspeadnudpotnhtrhee
{rnefsoulrtmstoifonapLrCovSiOdesdtbudyy h(eWiSlpdoinfseorI,ntTerhneataioingali,nLltdt.estPrcoojnecstetNruimobnesrs4el5e4c-t1e0d2w)earned0,a2dd,it7onaadl2t0oxpicoimty
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ai. Followingexperimentalstar, th tetmaterialwasreanalyzed andthefinalpurtywaslowerthan
riinllyreported.Thereforetheactualnominal st oncetrtonswere0, 18, 62and 17.6ppm ai
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PROS Treatment Groups
Group NominaGlmCson)cern PGernsouppr
T 2
Conta) 0 18
5 5
3 .
2 176
s s
Bip Miles Females
To ro Lo
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600365
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Wildlife International, Ltd.
Project Number454-105
"toEachtreatment ndconto groupcontainedfvpins ofbirds withonemal andonefemaleper pen. Thre treament groupswere fd disconning ether 18, 62,o 17.6pm a.ofPROS. The control groupwa feddiccomparable o herstmentgroups,butwithouttheaddinof the es aubsance.
AdultmallswereexposedtoPROS tpominaldietayconccntation of 1, 62ad 17.6pra 4. foraperofi6woeekds, Acontrolgroup, non-resteddit,was ain concuralywith he rcatmentgroups.Eachrotmntand hecontol grovpconsisted of fivepais ofbirds,housedwith aoe cmoanlceanrdioonnsefweeremcupatebralpnieezne.danAdttshubejeecntdedotfoWereoksn6e,carloptsyb.irdTsesinbtihdesi1n8ahnedco6n2tpolpgmrouapi.ateasdt 17.6pp ..ratmentgroupwereminaind ath aproprineditunt th beginningofWeck20of hetest at whichtimethy were ls ctbize and subjected 0grossnecropsy. Effo eaduclthtels,
bvoiadbiylwiteyi,gahttc,haanbidlfteyend dcoofnfssupmrpintgisounrwvievrael feovalalulatteesdtacsowneeelnltasisoenfsf.ectsuponeggproduction,embryo
Theadultbindswereabseveddailyformortality, abnormalbebaviorandsignsoftoric. Adult
bodyweightsweremeasured a st nation, on Weeks 2,4, 6 8, 102d 11, and atadulttermination.
Feedconsumptionfo cachpenwasmeasuredove sevendayperiodcach wecktroughs he test.
Nectopicawereperformed onaladultbirdsad on10 offingfromcachfetconcentration. Liver
weighs were recorded for al necropied birds, Lives, bile 20d blood (vben suse) nd feather
Kiadmnpelye,swgoenraedc,plolventicaltuhse,tigmalelobfleadsdoerp,eaydifpoospetoessub,eaanndalbyusirss. oAfddieoincilssa(mwphleensaovfilavbelre,)bwreir,e
;
xd in 10%bufferedformalinforBistpatoelxaomiinactiaonl.
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Duringth ts, themumberofeggs lidincachpenwasrecorded o value ggproduction.
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Eggs id inWeeks 1, 3 and 6ofth testwere collected ndseparateditshll,shel membrane,yolk
ahnedteasltbwuemerncanodlstloreaednfcrdotsgetenfdrfoipnocsubiabtlieona.nalEymsibsr.yoEgvgiasbilaiityd,dhuraicnhgaWielcyk,sacshndin6g(bDayse3a1nda0su37vt)iovf-
abiwerle aimitneyd. |
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000366
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Wildlife International, Ltd.
ProjctNumber454-105
"ue MATEARNDIMEATHLODSS "Thestudywasconductedaccordingfo teproceduresoutlinedintheprotocol, "PFOS: APlot ReproStddyuwitchttheMialloardn", Theprotocolwsbasedonproceduresolin intheEnviron mental Protection Agency's Registration Guidlines Pesticide Assesment Guidelines, FIFRA Subdivision E. Hard Evaluation: Wilde and Aquatic Organisms, Subsection 714 snd the ASTM "Standard PracticeforConducting ReproductiveStudies with Avian Specie1s2)".
"Tet SubstanacnedInternal Standard
"Thetetsubstance, PFOS,wasrecivedfiom 3MCorparaton on October29,1998andwas
sighedWild ToteoaionLld,. densiumfbeir4c67a5uptonireoceinpt.The testsubstawnacs8e
whitepowderandwasideatifiaesd: FC-95; Lot 217.Thetestmaterial hadan
989% nodbadexpiredat he timeofexperimental art. Ansayofthetest
original
teil
reportedpurityof
afer experimental
start ndipucrtyaof0e49d%.Thefoalassay ofth test teilindicted purityof 86.5%and
expiration dateof August 31,2006 (Appendix.Thetestsubstancewashedunderambiatcondition
Jockedstorageatthe Wildife ternationaLlid,. fi in Easl on,Mars yland. Concentroafttihotnest
`9su8b.s9t%a.ncDeiiantrhyecdoinectewnetrraetaidojnussateedetxopr1e0ss0e%dact1ipvearisngpreedieintblaoseadctuipvoenitnhgreeodriiegntin(aplrpempo.r.t)edinputrhietydoieft based uponthe filreportedpurityof46%.
Theienesander,4HPFOS,wasreceivedfrom 3M Corporationon July2,1998andwas
a`asgsriagnnueldarWimladtleirfiealInitdeernntaitfiioendals,s:Ltd1.Hi,de1nHt,if2icHa,ti2onHnuPmebreforr4o5c2t6aunpeoSnulrfeoceiicpt.AcTihd;eiCnAteSrnNaol.st2a7n6d1a9r:dw97a2s.
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"The itera standard was held under ambient conditions i locked storage at te Wilde Inemational,
i
Lid. filinEestso, Maryland.
TestOrganoms Fifty-one (24 males and 27 female) pen-reared mallards were purchased fom Whistling Wings,
oc, 113Washingion Sree,Hanover,IL 61041-0509, US.A. Ath sarofsclimtaetmailloar,ds appesrcd beathy and were phenotypically indisinuishable rom id type. The birds were rom the amebatch,approaching hee rstbreedingsssonandhad rotbeenusec i nyprevious estig, ALhe sant of acclimation, a random mumber geneaing function in a spreadsheet program was used fo randomize pen ssgament for each bir. Inmedinely prior to test tation, all potential study bids
600369
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Wildlife International, Ltd.
ProjectNumber454-105
"nwer examinedforphysicalinureadgeneral esl.Birdsthat idnotappearety, ithe deto injuryorinabilityto acclimateto abaatorycondifons,ower ovtsidetheweightrae orth test,were excludedfomthestudy. Albirdswereapproximately27weeofakgesa tet nition (istdayof exposureto festdit)andrange inweigh rom 788 0 1258gra of est nition.Se ofbtidshwaes determinedby 8visualexamionfathetpliumoagen.
[r-- Adult idswere enti byindividualIgbands, achpenwas denied with iq mumber,
andgroupsofpeaswer deniedbyprojectnumberandconccaton. Duinthets, the umbeorf egg1din achpenwasrecordedto valuate gg production. Al eggscollfoescamptlinegodr incubation weremarkedwiththepe mbesuso apermaneotinkmarkingpenfor deat. Hatelingswer naly eatifie bywingbandss tat heycouldbetracedto heleparental penof origin. Wingbandswere removedandth offspringwerebanded ith egbandsatspproximatclyfour wks fag.
Avian FeandeWatder A adult bindandtheeoffspring weregivenfodand eater od bin during acclimation and
{esting The bas it 1 t both adults and offpin was formulate to Wile Itematioal, Lu. specifibcyaAtgviaoyLnas.(ppeIo,Tdbli1s.Thebasal utioncontainedatlest27% prt sad 2.5% fat,andnomore than $6 br
Thebasal iecontainedapproximately 1.1% calcium,derivedfiomfocdstullsandthe 09% Iimeston sed ntheformofuthlbasaadtiebiyAogan. Whiletis evelofcalcium isuiftor growthand maintcsncerations, additonal calcuis rogueinthetoofbreeding bisfor egg shell formation. Theraneadfitoonarl 5,% (wiv) oflimestone (spproxima3t8e.l5%y C)wesaddedto he basa dit for the aus This raised the calm leveli th diet fr the breeding bids 10 approximately 3%, sigty sbove the minimum recommendedfo qusil (2.ao3dma%ll)ed (2.75%) G3). Offspring recived basal it without est substance 0d without the addition of $% scplemctal Fesone.
Water was suppliod bythe townofExson public wales supply. Neither th adults or ffpriog receivedanyformofmedicationinthefeed duringthe est. Foodandwatewerealyzedperiodicalilyn accordancewith Wildlife nemstional, Li. SisadardOpera Procedures.
.
000368
Wildlife International, Ltd.
Project Number 454-105
"iDiet Preparation
"Tostdeswerpreparedbymining PROSito pei hatwasused forweeklypreparationof thefaadie. Controldietand ach tedditwerepreparedweeklybeginningonFebruary 23, 2000 andpresented 0theidsnTuesday ofcachwek.Dietaryconcweereaatead fiorpourtnyof the est substance andar prsened 8spartspe linative ngrdint(ppm a). Detailsofthe `weslypreparationof estadcontodit reshownis AppendixIL. Diet Sampling
Homogeneity ofthetssubstancefnthe detwas evaluatedbyslletiogsi samplesfom eslof {herested diesan onesamplefomthecontoldiet onDey0ofWeck 1. Sawmercpollecltedfeom. {he top, middie and botomofthe let and right scion ofthe mixing vesel. Control and treatment rou diesampleswere ls collected rom hefedtroughs on Dy 7of Week|(0assessstabilityofthe
testsubstanceunderactualtestconditions. Additionally, asamplewascollectedfromthecontroland. treatmentgroupdietsduring Week 6ofthetesttomeasure/verifytestconcentrations. Thedietsamples
were stored frozen or transfered immediately to the Wildife Intemational, Lid. analytical chemistry
facility for analysis.
Analytical Method
"Themethod se fortheanalysis ofPFOSin avianditvsbaseduponmetbodlogydevelopedat
Wildife ternational, Li. aad ened "Ansys Method Vetofrthe Determination of PFOS
in AvianDit"(WilId nternatLuid.oPrnoj,ct No. 4$4C-110).
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Samples were extrctod with methanol aad ited in 3 50% methanol: 036 NANOpue wer
i
sSotlauntidoanrdc)ontaanidni0ng.00%.fo0rmm1igc01aH0c,id 1(Hv,4)2Ho, t2aHt tPheeryflufeclolroictlanie tShuelfcoanliicbrAatciiodn r(a4nHgePFoOfS;theinPtRemOaSl
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st`amnetdhaorddosloagyn.dAxmteatthsodofflotwhcehasratmipslpersovwiedredeidneAtprpmeinndeidxbIVy,rFeivgeurrsee1-.phCasoe hnicghepenrtfoorrfmaPaFntcOieSiolnintqhuseid
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chromatography usinag Hewlett-Packard Model 1100High Performance Liquid Chromatograph (HPLC)
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wilh + PerkinBimer SCIEX API 100LC Mass Spectiomeer equipped with a Perkin-Elmer
i
TusbolonSpry ion sowce. HPLC sepations wers achieved using a Keystone Bets! Cy analytical
cAoplpuemndnix(5I0V,mTmalxe 2 mm LD, 3-um particlesize). Theinspat ramer teru sarm esume marn izedt in
000369
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S Wildlife Internationale ,LLted,. TePr, oijeectNNeumbbeer 4s54s-1s05s ue
Calibration standards of PFOS prepared in a 50% metha:n5o0%l NANOpure water solution
ccontoaininng c0fr. eom m0 n0.g01 40t0H40 P3r9F0 tOoSa0.0(ti0n4te3ir9nmalgosatanLndar(dyW/eLekan1d, D0a.y050)%arfo0r.mi0c03a1citdo (0viv.),0rr0angg4ian3gi9.in
s(aWmeeeak1d, Dmoasyt7p,Wereko6,Dpmeaayk0ireasnpndontsheeefsoanmPpFltOeSrew.aesxtructtiiloiznesdtto,mwoneirieor PlyOzSeidnwailthtliebsratmipolne,sq,uTahye
ccompoonennntstd.Ltriandeoasramrlepglre,ess.siNoon etqueamtipotnswawsemragdeecrfioeqduaunstiintgypPaFkOSorcnehsepboaussiesrofoisndi(viPdFuOaSl
isomeric erm
stsatannddaarrdds).veArnsuesxtamhpelreesopfectcailviebcroatnicoenncturravteioinrparteisoesnt(ePdFiOnSAppeinndtiexmaVl,sFtiagnduarred2).oTfhthceocnacleinbrraattioonn
sOafmtpelsetssuibtsottanecepipnltihcebslaemnpleeaswreagsredsestieonremqiunaetdibony,sTuybpsitictaultiinogntchehperakoarmearaoesftploonowseagnradtrihoiasgohmfletvsheel
a`rcealpibrreatsieonnsttaeindndaArpdpseanrdeixsIV,hTiono leApw 2p.enn dix IV, Figures 3 and4, respectively. Examples ofcalculations
cal"Tchemauetlthhoaedptlroiedutdcotoffqhue laownesttc(aiLliObtrGa)taifoontrstthaineWdoaercdnakna1,lyDzeeyd 0(ana0lys.is0mwags0se01t )a4a0n.3d3t799hpepomveraail,l
dWieleutki6on,faDcatyoro0afntahleymsaesraxn.btlhaeksasammpplleere(-2e0xt0r0aLcItKiogn)s.eTthweamsseet atLtO14G1phfoprmthoe3W,edcealkcu1l,atDedr73atynhde
lPraondkucstaomfpltehsl(o4w0e0s0tLsKta)nd.aErdxaanmaplylzeedof(0c.l0c00l3a5o1nmsga.re.pLe)satne
the vealdiltionfcr inAppend IV,Table
of
he
me
Alongwiththesample.analyses,fourmatixblankswereamlyzed todetermine possible
i
in(tAeprpfeenrdeinxcesI.V,NTaoblient3e)r.feArentcyepiscwael roenocbhsreormvaetdaogtraomraofboavmeatihseLbaOsQkdiusrpirnegsetnhteedsianmApplpeeanndailxysIeV,s
|
Figures.
|
41d 2A2v0iapnpdmietais. a(mWepelkwee1r,seDfoarytif7i;edWaetek0.867,9,Da8.y790, aanndd22t.he0 pspammpal.ei.r-(eWxeterakc1t,ioDnayse0t)) nord1.s7n6a,y8z.e7d9
I
pcrooncceudrurernatllyrweciotvhetriheessoafmp1l0e5s%,t1d0e4t%e,rm1in0e7t%heanmde1n02p%r.oceTdhuersael vraelcuoevsercyo.mTeshpeomnedtthoocdaycihelsdaemdpmleeasent.
i
analyzed or reanalyzed during the defiitve study (Appendix IV, Table 3 Sample measured
000370
_--
fbd Wiildlifbe IinterrnattionalN , Ltd. Pr. ojsecttNuimberr4s54-10A5
ase
copnicceantiuotniocnswherreoaotmcoofametmtaofiogfrtirecrtaeispomenctiisvpemersaeprinosAcpepereandnirexcVotv,eFreyiogufdrtasuple et. A
HouandsEnviironnmengtal Condidons
tbe
Housing sad shan NationalResearch Counc
p(r4)c.iTchsewedruecboinrddcswieedrhousfeodiandbdroeorfoitnhbgautideelsionefspeesatsabmlaismhuedtbay.
"TuhredpbenysSawfeergueacrodnPsrioedteudco,fnvci.ny(lM<oodsetleNdow.i$r3e5m)e,shm,eAasidiaaggarapmofrthoe txe7ti5laXymo9u0tsXi4ir5eccsmtlheidgyfhn,
AppendiV.x
placed
Exch i the
pn was equipped with fed rough. Weekly, tough fo cachpe andpresentedtothebids
sufcit fod for the fein period was During he feedingperiodadifonalfed
5aweighednd added10 he rough 8needed. Waterwas suppliedbysipplew-atyeprse.
excessOinveldyisbuisrbdasnacsesso.cTiahteedawviertahgettheimspsetruadtyuwreeriethmeaidnutalimnaeldlianrdtshteudstyrudoyormouosmiinntohre cdoeurrtsoeaovfotihed sfeyssttewmaisnt25h9s4t0y.rCoom(SwD)aswidlehsigannevdetrogveetruelpaitvoe 1h5 uoommiadiofiv4tl8uy%ne+s1v7e%ry(hSDo)u. aTnhersevplhaacnedlhienmg withrshair
rinTg haceclipmhaottioopnearnioddihnettheesawdauslt1m7aloluarrsdorfoolimghwtapsemdaaiy n0ienddubcye gatgimyeincgl.ocTk hTrheopuhogttohpeoeeruitot,d
| |
thebidsreceived amean ofapproximately202lu (~ 18.8 .candle)of resent lightsthtcloselyapproximatednoa-<daysuligh.
minationprovidedby flo.
Observations Thetestbidswereceimated othe lisand sadlyposfospprosaiey 4weekspriorto
i
Sinhiaoonorofdetihelatdeti.ng Dpuhyrsiincgalacn climatiowner , ralnlotbuirsdc sdwoerehobtseesrt.veDdudnagi.theBisrtdds,eaxlibatcinugbiarbdnsowrmrael
robosmerhvetdcdiingyufonrsitgenrsmionfattiono. xA irecioarbydnwooarmsramlaibnehtaaviinoer.d fAldldimoaoly,rllaafncllrsiscwlieorbesoenrbvsaetrigovnesd. diy
000372
--_--
LWiLldlM ife Ie nterHni aAtiNonAaN l,,LLtee d., essProjes ct Numbei r 454-10e 5 s 6-
AdultBody Wei1gndFheetd Consumption
terminAatdiiont.Fbeoeddywceoingshutmspwteiornefmeoarscuarcedhpaetnetwiisnmieoansu,roend
Weeks 2, 4, 6, 5, 10, 11 weekly thoughoutthe
and a est,
ak Food
acdodnisiuomnpstiodinewtaasdddeetderdmuirinnegdtbhyeewelekg,gantdhewefiigshhiyngltehde
fod onDay 0, recordin feederand emsininfoe
the at
mova ofsy he endofth
fecadsinnogrmpearlioyds(tDaeye7d).aTnhdemaimxoeudswtiotfhwfeaetdewasatneddebxcyrtehtea.bTihderwealrseo,tfeqeudanctifoiednsscuetimhseppwraetssteiendteofdosonds
an Of
etimteoftot eed Weck20. Therefore,
consumption. Allremsiniginswereeuthanizedtredaysater mofod consumption timatewasclclte for Weck20.
he
begining
AdBlooud Colllecttion
FolloAintgtchoellecatdioonf Woefebklo6o,dbslaomopdlseasm,pbliersdswearetchlelc1t8eadnfdrom62alppsmuvaiiv.ingrbeisrtdmse,nwt hgeronupposswibelree
acduhtaneize1d7..6pAdmiti.o.nralatlmoeontsgarmopulpepsrwieorreatoseocaosllstewhhecebetpgoseisnidibnl,eg,offrWoemcbkird2s0i Atlhlebclooordslamgprloeusp
weresepartedintoserumand hemacycslatles,stored Laborsrisoc,. forpossibleaml.
iozen and
shippedtoCtr
Analytical
`AdultNecropsysad Tisme Collection dislocatAionh,enccornocplsuiseido,naonfdthseteorxepdofrsoezpeenr.iodA,tatlhlesutiivmienogfadnuelctobpiedys,wesreucsutwbneiezecdoblyetceerdvicfaorl isasmoppluehsoolfoggialallbclaxdadmeri,naliovenr,(0prnovdent1r7i.c6upluso, k.i.dgnreyosu,psbornil,y)gnondadpso,ssbiublressofyFsab.riciWuhseanndvadlibplse Hsiusteopwaethroelofgiyx.ediWnhe10n%bavuafifleabrleed,fsoarmmpalliens.HofisboilleoagnydsalmipvelrewsewreeesstoirpedpefrdotzoenEPaLndinshHieprpneddonf,o VCAefntoer AfneaaltyhteircsaamLpalbeorawteorriseso,rIecd.ofoerpofssoiboleatnaalylsisa.lyAsniysr. einissiunontingedforhistopatholaongyd
EggColleacndtSitoorange
calFcdisdiyidfdruormintgestapseenvsenadndaysptoerreidodinb3egcionlidnrgooomntahlseicncounbdadtioyn.
of Week Eggs to
5ofthetestwere be incubated wer
washed 0recethe posofpsathoigen contamination befor soi ther inthe old room. Eggs
:
600373.
---
T Wildlife InternatR ional, LLted,, A MPeroejeNcteNubm, beere4s54t-1i05s
-
cionlleccotmemdefrocritahleiogng wfaousrhedra(yKsuwbelrEegwgaWsahsehdebry)whiatnhdapichriotroinebloroagde.dTehcergeemni(KsulSougpgerwCeDr)e.waWshaerd
fiontrhsepwapsrheorwxais1mw5aasrtemceeodnltdosy.ppThreowxaishmear4't5cliry.cuTlahteioengmgostwoerweapslatcheednituthreewdaoshfwoataeprparodxismoaatkeelyd
thfreeeminumteasp.n iTehneeedrgwgisthtwferhrerweasmtoevreTdhfecoomldtho.owmawsahesrm,aailnltoawiendeda0tc8omle
t
spproimtdly temperateof
room 13.1 2
0.1C(SD)with meanrelativehumidity debyn anali phabeteicofdcod. Alleg
ofsponse 75% + 4% (SD). Groofeuggspwesre iddringtheweekwerconsideredsane ot.
CananddIncuibatnion Ath endoftheweeklyinterval, leg wereremovefomth cdoom,covatedandcandied
CWriatchkaedSpoeresbdoKoimnngal(eMgogdsewleNreo.rec3o2r)doegdga-n<adnddiisicnagrldaedmptodetect ggshellcracksorabnormaleggs.
the femAplelretgugrsewnaotsdimsicnaradnedewdeartepanlavceerdigne37.P5e4e0v0eCInc(uSbDa)twoirth(aMnodveleNrog.ewSPe2t0)u.lInttehmepienrcuabtaetoofr
30.6: 0.1C(SD) relative humidityofaprosimsely60%). Theicubaorwasequipedwith apulsetor
ftaDeenmllapmneedmrbbslraaadnneeds,ttuhhneatiipntrcouybdvauatcroieradtwiaoasnmdirllidoogberqeiinacptuphbieantgiwoani.itIrhnmaoovrsdeemrtetnooptrdteevseigngtsnaedrdohtetastoiiooennldioemfvinihacetece,irdnetyrgarcaetboitfnheeot
r(olttaateatrheeofggrostfartoiomnw5e0sof10f0of)evveretriycatlwionhoonuerdsirtehcrtoiounghtDoay502offofofinvceurbtaitciaolni.ntEhgegsowppeorseitceandidrieecdtoionn
i
Day14ofincubationtodetermineembryoviabilityandon Dy21to determincbryosurvival,
menireat ---- | Hat1c2dhBroaodigng
allOtonDowhaaeytc2dh4.ofPiencudbastibona,sktehtesegcgonsswtreirceteldaocfedgailnvaaneitzeerdssteelHawitrcehemre(sMhowdeelreNow.eSPd-6fo8k)eaenpd
femipcehrisntgusrsepiun rthteehobyb parceompapretnmoenftworaisgin3.7.2E=gg0s0weCre(nSDo)t,raondatehdeinavetraegehwaecthbeu,lbTtheemapveernagtee
|
000378
_--
--WildliLfeLTInTtTeArTnaItOiNoAnaLl,,TLted..
PProojNetaNmumbbeer4d54s-1l05s
Le
incAublal.baTchheignrgosu,upbnosdcyhweeigehtgo,fatd urvsihvelilgsvecrlsirnegmsobvyepfeonmwasthedBetaecrhmeinreodn.DDuacyk2li7nogrs2w8ooef pawriensgtbaalnodendcfeoaidoentifircoaptiinong biynpbernodoifnoripgeisnuanndlhpepnrrooiutmiantellyyHo3uwseeedkssocfosrgin.g A1lltdhuecaklpipngrspweenee dmuockvleidngswpeenesfwdituhosuetsudpdplieemtewnittahlobuetsth,edwhieroenthoefy 5w%erspehploeumseeadaslplpirmoesitmoanet.lyAt9swperoeski,mtcTlhye
12weeksofage, theaveragebody weightby parentalpenof al surviving offspringwasdetermined,aad
tfhoinercbiniMerrodatosidwonea,rnedeuttahamniezseadwmpiltihncgarwbeornedisotxoirdeedafndodinspfoosleldoowfibngy.nienccirnoeprsatyioann.d`Tholseeroffdsipsrpionsgesdeolefcbteyd
EschHpaetnchlcianrgewdeprerouos ixabia62tXtme9r2saoXftb25r.oe5ocdimlngipgyehs,Tmahneufwsaclu,refdlboyrSnadsgeulsndgsProofdoecatsc,hpnec,
ssweeevtr1e0cmodanaisyntsrtuaociftnegdtsoe,fmwphveiernanyttlu-hrceoatotefemdpawepirparrteoumireezswthee.slTyhae8drimsCoisetdoafmtosaihnetihteinbieroofotdieenmtgpcehcrionsmgpnaritolfmstepnetrboeifdesawcehrpeenf2iw9veert,eo:
TBhroadaivnergawgaeasmdbiisecnotntrionoumedotneompehriacliinngstwheerroeodmeehmouisnieagdotfofbsperionfgsvuafsfc2i3n.t5 iz0e610Cth(e5rDm)owriethan
arveenrtserdela0tpiveenshuimihdoityofsu7p1ple+me1nt1a%l (bSeD)s.t Asplprduocxkilmiantglsyw3ewreerkesmoovfeadgef.romTbrodhpieengsepweesreahned
salomcekmatod1e6lbuoswesdtfohiougspetehhetaayd:ultbirds.The photoperiodfortheoffspringwasmaintainedby a time
i
rOfafsmperPitrnggoBrlo1ou0po.cduatAnbldnTaisosisoauodefsCatomhlpeleloceftsiwfoienneg,sbelpoaotdedsaimnptlesserwuemre0cdolhecmeadcfyeosmlat1c0loef,fssporrinegdinocanc,h
i
atnhed1s0hoipfefdi0ngtihCecnatcrheAgnraoywaer!eLcaolbleocrtetdrfiorcoBsci,.opfaotpoolsoigbilceasl acxlumai.natiAondnddiatyeos.nueasWflrho,em
aivapioable,issameplweesorfegaflxledbiadnder1,0%libveur,fpfreodvfeorrmaal,inaKindodeyss,hirpiend,togoEnPadL,iunssHeoafdeFarb,ricViuAs afnodr
AHniasltyoptaitchaolloLgay.boWrthoerniacvsnac,i.lbolre,psoasmspliesfyisl.an er issueweresre fae ndshn ippedtoCen
.
000379
---
-- Wildlife InterOnatL iona,l,CLtWd.
PProoejeNcteNmumbbeers4t54i-1o05s
"-
StatinAnsiyses
ussteaditsUotpaclooymnspcaiormgpltaehtnitodnhoieffeftrohcseettsmesebtn,etwaenmeAennaganlrsyowsuiipst.ohtfDhuVacnroninaetnr'coem(luAgrNloOueVpmAce)awonasampdeprfspoarrsomcsereddttuhireoe(dss5eu,tie6orsmwiinacnels
s01Di, egxepiotfbtofedoybisnedrclvievdeardwiefnirgnhcctessw.hSearmeptleeswaimtpslweeureitthwe svhiedinadipveindsuawlitbhiirnds.aPehrcepnetraigmeedntoanl
swteirteicaxlannilsyesdesswienrgDeuconnndeuc'tsedmewtithhohdefbooldlyowweiingahrtsaindefsoqudacroenTsuomtptisofndoertmaa,iOonne, sTtwofossmelsoyfs
oalnlyrloeoekedeaatmbeondtygwreoiugphst.1Tnhdfeseedccoonadssatnopifoanrdlyt
rom hefs 6week nly vaunedthe
ofth studyand contol nd 17:6
cvaomed ppm a1
croempsaernitsognrporuopcseadnurdeweaxsamnionecdodnastidaefroedthaepfurlledsatiocnomofptareestthudcyo,n2tr0owlereokusp.Dtunnseintgtl'esmeanlieg
co5ntpr.olTghroeusptauddentth'es17T.e6sptwpas.usredetsoemnatkgerostuaptwisetiecaclcoommppaarries,ons ithos stanceswhereonlyte
pte | 1 `Ad1u0l5t,7B00op1da1,yadWnedicaagcthhatwd~aiItntmdeitvenritmdiungaarltobiuoopnd.sytSwceiaichgshwatewilgachsoimmnepgaasrueirsveoadnalstwbteeysrsteixnmfitdoiaettibhoene,ftWwseeenktswh2ee,eck4,osn6,t5oI,nl
arddeianm,sgraoupionecwomepsar5i,s1o0n,s1w,eraendm2a0d.ebetwenthcontrolgrovpand 17.6ppm i.
2Adal FedContumtion Feed consumption expressed a grams offodperbidpedywas
|
ceoxnatmrionlaenddbcyapcehrnswetemkelnytdgurroiunpgftohrewteeestk.s `S1ttartiosutigchal6.coImpaariisonns,weareimasdeecboemtpwaereinstohnes
i|
3.
EasweLraemda-dTebheetwneuembnterheofceongtgrsollaindd eprodicive pesommanc,dtws
p7e.r6pfepmmaal1e.petrreartemesnetgnrtoguprfoor.wFeeokrsth7ethervoauglhua19t.ioofn kenfomweek eggset
|i |
.4. VEixableCEncmkberdyoosfE~TgghseLnuimdberTohfe invumebmebrroyfoesgdegtserdemtienremdianteDdbayyc1a4nbiyncagntdloibnegcrackeddivided oy he mameofca id,pepe
|
i
6. VileEmbryosofEggSi -Themambr ofcnbryosa heDay14cadingwasdividedby he
|
:
60037
_--
Wildlife Internationnaal,, Ltad.. .-
PProoljcetNNaummbbeer d45s4e-1s05s
7. Liv`ew3asWdeiveikdeEndbnbyrtyhosuomfbViesrbloefEvmibbrlyeomsb-rTyhose,npuemrbper.ofliveembryos atthe Day21caning 8. `Hatchdliivnigdesdobfy3h-eWeuemkbEemrborfyfiovse -3~-Twhoecknumbbreyrosofpeharptcehnl.ingsremoved fromthehatcherwas
9. 14:Day OldSurvivorsofHatchlings ~Thenumberof 14-dayoldsurvivorswas divided by the `number ofhatcpherlweeik,nbygpesn.
10.`HatchlingsofEggsSet ~The numberofhatchlingswasdivided bythenumberofeggs setper weebykpe,n.
11. 14:DuamybOelrdoSfuergvgisvosrestopfrEwgegeskSbetyp~eTnh.e numberof 14-Dayoldsurvivorswasdividedbythe 12. Offispurrivnigv'osrBswoedryeWmeeiagsuhrted~Tbyhpeargernotualpbpeondgyrowuepig.htsofsurviving hatchlingsand 14-dayold
13.LivoerfWoeifgfhti~Ipondfriwviiedeunaklgl5i.veSrwteaiighstas!wceormepmaeraissounrseodaftaadduullttltiervmeiwnaetiiognhasndwsetrtehmeatedremibnyastiooxn
betweenthecontroland reatmentgroups. [EE --------
Juvenileliverweightswere compared by
test
Adultmalts
wer
exposed
RESANUD DILSCUTSSISON to PROS aoi icanconcentration
of
1,
62
0d
176pp
tre1.atfomentpegrriooudpso.fB6awcehetke.smAenctoanneltghreocuopn,tfreold ngnro-urpecsoinseidsdteide,ofwaisvmeapiatissionfedbsirodm,ehaoruseetdy witihhantehe
maleandonefemal concentrations wee
perpen. euthanized
Atthe endof ndsubjected to
Week 6,adultbidsinthe grossnecropsy. Testbids
18 i
aad 62ppm a. thecontrolgroup
tat and
17h.e6 epspt,matw.h.icrhettimeethgeryowueprweorlesmainttbaainniezdedaontdhesuapiptreopdiatoegdrioestssnneecirloptseyb.eginningofWeek20of
Analytical Resuts presenNcoenoefocfo-tlheuicnognstuoblstsaanmcpelaestshhocwheadracatneyriisntdiiccaeteinotnioofnthteimreosfctcheeotefst hseufbessttansceu(bTsabtlaenc1e).ofDhie sawmeprleasnawleyrzeedco0lleccvtaelduftoemthtehheo1m5og,e6ne2istyndof1t7h6tpestnsauibs.iaenctecionnthceedaiietoannsdotno Wveerikfy1,teDtasyub0s,aanncde concenation. Meansandsandrddevonfotheths estcomcotationswere 1.8 0.13ppm,
000376
---
-- Wildlife InT ternr ational,ILtd.
PMrkojeecNteNummebedr s45t4-o10s5
a
a6n0d0.836%5,pprmespacit.i4v0edly.17.T6h2es1e.4 vaplpumesa.reprreesspeecnttievdely1.00,Th9e7oand.s100f%ofovfcanoimiinnalweecroen7c2enn5t6r,a1tsi1on%s
c(ApopenndixcIVe,foTanbtlheet41).,r5,`S6a.a2maptlnedsi1c7o.lo6lepcntpemdsiu.ridniegtsWbaedme6,aDnasyad0 osfttanhdatrdsdevtiovaetriiontsytoeft2s0ubst0a0n9ce7
P4P6% 041,1%60nd 30.6%p,6prems7paeict.iavnedly.1T6h8ese06va0l8uepsprmeparies,ernetsepdec1t1i1ve,l9y7.aTnhde95c%oofneomifnoailfcvoanercieannttiroatntwieosrnse
(AppIeV,nTadblie)x. Analysis ofdic tempers for 7days averaged 89%,87%
asnamdpl1e0s5%coolfletchteedDfaryom0vfeaeudeerfsofr eieb1e5,g6h2elnddst17
bic ppm
in
tetcocenaions, especie shown in AppeIVn,Fdiguirex7
(Appendix
IV,
Table
6).
Atypicalico hromatogrfaam
test
sample
MorandtnlealiObsetrvatieons couoNfortahsedtleetstm.aServeiraelboicdcsuwreerdeniottehedcwointthoblumbrloe ooofifnoatalyeosfiotnhs)eanedsethte glroosuspshiregltohef preantwmeeanragnrdouoprwpaessnomtaeedwaigtgrhesseioanddliesoiont.hAenicnoiudsentofathleitsesat. iAg,siInmgleenebsi,diwnsthaesso6ci2aptepdmwi2th the bumble ot.
nth 17.6por copedfr Bcaing
ait.tersetstmeernmtinagtrioounp., tShehenlfrooemxphiebi2t1e8dabrocdhyetdebemronrescaknnddbaepcdamelirnikgiindwg hanedn
"Tehsipsihneiown dsaiobnserhveebdl10ebdeinnoprrmoacleisn.sCplpineiacnalcssiagands
pee behavior
1 be prio
riggedby theses ofbanding, totest ermination. All otherbids
nlltest roupswerenali spesraceandbehaviorforthedionofthefest.
`Adult Body Weight andFeed Consumption
bodywWehigehntcatomipar18eo1r06t2hpcpomnatio.ltgersotucpotnceernterawteiornesnanodaapnpayriefnftetesatcemsebaettweeaeentdhoefcfocnttsroolngardouultp
pan<d0.6025p)premduac.titoentincfoenmcaeltebtioodnywweerigehntoattathteis1i8apllpymsi.gnitfiectacnt, oThpereewsefrnosmitrWiesaeikalt2yfoisWieogcnkni4f.
|
THheorweweerrimeraendubcotdiyonwweaisghnotocsoensiasmteonntnomraldeossa.raesfpemoanlsesiaivnnetdweas17n.o6 pcponmsiid.ererdetsreeantmtegnrtocuopthet.
may avebea elatedt0restment.Whencomparedtothe contol grop,meanmsebodyweightforthe
_--_--
L Wildlife InternatT ional,LLtdE. T TPoroejcNteNuem, bsers45i4-e105s
a.
17.6 ppm interval.
Tah.eercwaatsmenltsgrosuipgwniafsicaingtiIfsiscoanftvy(epa<lm0.0n5)bloodwyewreitghhetWfoereksl1e0s,
1130d20bodyweight t he 17.6pm a,txt
dicfofnrceenncaetiforno.mWthhielceotnthreorlewwaassnaotsovearacllllossosfgmaiefaicnabnotd(ypw>el0.g0h5)f. oMfeaenblodiynwtehiegh17t.m6epaspuream,enhtes
ar prescotedinTable2,adindividualbodyweightmeasurementsaepresentedinAppendixVI
when Dcoumeptaoreexcde1s0sitvheewacsotnatgoelbgyrsouopm,etbhierre,sfopoedacroendsumopbtieon nwoatsevsatiesbsle beeltawteeednpfsf.sHoownevfeorn, co9n<s0u.m0p5t)iodnifafrteence15b,e6t.w2eeornt1h7e.6cpopntro.l.grtoeutpcaondcenhterat1i7o6nsp, mWhi.l.erhereeweragersotutpifsoimrcWlleeyeskisgn1ni4f,it1ca6n,t 17o,u1p.8 aFneed1d9c,otnhseusmepitfiroennmceessswreemreendtseat0 ahpepa1r7en6tppcreis.tsisnfceodcnocnseumnptiwoen rfcoorntshies1t7e.n6tlpy pigah,r rtehsaunltthoefcfoonotrdowlagsrtoaugpew.ithMeacnomfeosopdocnodnisnudmpetcieosnsmeiansbuoredmyewnetisghatr,esusghgoeswtniogin hTeaibnlcer3e,asaenwdsfteehde consumptionmeasurementsbypen cepresentedinAppendixVIL
Sy frves iol wf. eof st.sie | GrossNecropsy
AChendof Wek cubnizedand subjected1
6(Day42)al adultbids ith grosnecropy.Adultbidsi th
18aod 6.2pp ..etm groups conrland 17.6ppmi.retment
were rope
wseruemabitja0ingeerdocsonntetchreeopppsdryRo.piAsElt fdiendsiwngsiobtshe-- erveegdiwaienrgeocofnsWiedeekred20taobnewienrceidtenhteanlefutohaniaztemdsenntd
ca1c0jhuwveeanmleebnitrdlsevoerl cwiatchhtbetugrmoufbopowtle(rfeeotseounsb).jtAogderdocsistntencareolps,dy.eNleceroopsfypevreiisnlndhgseev1e7r.a6lpbpimdai.n
briredswmaenstgnrootuedpwwiatsh naortteidwnietdhypoilckksedsa,ndbrroeknentfesotfhtehrespaantaaynolkksecn.tOspnpeemaarlacee,offIspnrrinngainltlhhyee
|
17.6ppm a. eatengroupwasnotedwiththeposter observedwereconsidered obeincidental 0treatment.
orion
of
ef
Kidoey
sheet,
Alfindings
|
.
000378
---
WildlifeT InterT natio, nal, Ltd.
PrPorjeebcteeNuNmebmebre4d5s4-e1n0o5s
ae
Histopathology
Lightmicroscopic examinationwasperformedonselected tissuesby aboardcertifiedpathologistat
`Experimental Pathology Laboratories, Inc. Herndon, VA. Sectionsofliver, brain, kidney, gonad,
de`xplraomvietnnatstriibcouinld.uss,agNdaoll rbloleamsdidoe1nr0s,oafcdpoinrpsoiisndegetr(iessdspupeprsoo,ssiaimnbsdleb ylyreu l1ao2twer fdefeatbs korsicsoa iedusaatm(ewethnebnaawnveaariselasbmflreoo)tmweciarncehtcloivlselre,crtoeKdefyrfoo,rm
opfrfoivreinnvgiulausa,nygaofltblhaedcdeor,ncovearny,rbartieostnaedsd.buArdsditofofnaablr,itcesroefwedruel2m0lesi3onndscfoenmsaildeesr,edortto bee
treatment elated mal ffpring.
notedin
adipose
issueofadult
mals ndoffspringofbothsexes,o
fnthe estesof
eiWtheinatchoiegmhpdearrienc1i0detnchecofofntaorlegrsoucpo,nstiesstteenstowfiahdpulotsmtarlepersodtutcthieve1p7h6apseprmegire.sstieosnt.cAodunlttmailens
iinnctrheeas17e.i6npcimden.c.eogfrotuepstlicsulaerxhriebgirteesdsiaonn inncdreaadsiepdoisnectiidsesncueemoifcradoivpeoisceulsauieonmficocaodeuslitumlatleosn.
The the
17.6 ppmai. rcamentgroupmaybeincidentalto discoid.Th fllpathologyreportisprovidedin
restment but Appendix IX.
a
eamentrelate
effectcould
otbe
Thrue Analysis
Exyge"nTRheesaenaarlcyhsi(sfoofrtmehrelygkgn,owlnoeo aCenndtresAunealsyatimcpalleLcoallebcieoddurarinnadgatrtheereospotrryteiwd eseeeparcsaotne)ldyu.ctBeldoboyd
,
saenrduvm randanqaulayitliclailvreresftosaanraelyrseipsorwtiendginH"PLECt.rEalcieocotfroPostpaMsrasaisuysm SPpeefcutroormoectrtya;nCseanltfroenStteafdyomNuQmubaelr
02341". The egg componens Perluoroocunesorate fom Egg
analytical ress ar Membrane, Albumen,
repored in and Yolk
"Extraction for amalysis
of Potassium using HPLC.
Electrospray/Mass Spectrometry;CentreStudyNumber 023.065"
'
000379
---
Wildlife InternationaalL,LLdtd,.
PProojelcet NNumebmere45s4-s10e5 s
ae
ReproductiveRests
Whit og ffoctsuponcg
production production
watasahnyigohfltyhvearcioanbcleenatrmaotniognhsenesst,edh.erAenwyeei50fappfarenbetetwreeeesnttnmhcenscteolmasted
10 ber
22d was
atrheeducctaiomneingergogupprsowdeurceonnosttstha6s2spymsi..graena,mnWthelvnec. Hoomtwopttehiaescrveodnruetcteirognrwdo,us,
oo4.ttdoestecornecsepnotnrsaitvieonnsdwwaasscnoomtpcaornasibdleerteodoelraetxedceteodreedtsehntc,onFripglgrrouop, dMeuantcceg1t.8poiroddo1u7c.ntpibseoynn
concenraestenitedoinTnaiblse.Individualeg production dataar peesentedinAppend X.
When compare viaiy,btchbiy,
tthe contol group, btchling bests and
therwer 10spare surivabily tthe 1,
eamen ltdft onery 62 or 17 ppm a. evel, Any
iffeacsbetweenthe contol groupadthe Parmetermessed,While herewer ie
atmnt rovpswere 1-dayoldsurvivors pe
notstitial significantforany henproduced tthe 18and 6.2
TPePvesa. .Retecopncenrtrdaatotiaoandse,tsueumsmcarreidtzcetdioinnTsvawbelree 6d.uRto feowepreggdrsabtefoionisdnedtivfuiodruinccpuenbsaitiisopnvroetseehne
inAppendXiIx.
OffpBordyiWeinghgts heeeauTnheenrtewgreorupesnaonsdppnaycdeitffterreeantcmeesnftroelmattheedcefofenctes ugproonuptwheebroednyowtesiasgihcsaolflbyastihglniifnigcsainnt,anTyhoenf 1w7e.r6epaplsm 5a.0atppraeresntmrgeerosnuetpn.tOrffesparidngfbsocdtyswuepiognhtthdebtodisypwreesiegnhtteodfifnvTeasllee7bsinddsAippehen1d.8,X6I2on
Liver Weights
HverwWeihghetnactaonmypaorfetdhetc0tohnecceonnrtorlagtrioeusotpne,sdt.hTehreerweewraes20saapipsacraenltlyrseisgatimfeiacatn
lated (9 <
ffcts on adult 0.05) incrosein
mwecrehfiegmhaelraemiovenrgwmeailgehst an hefm1s8laensda6t2hep1m.88.. 6e2.s2tpcpodmnace.retsotss.coWinlcemeeannatwdhruelant cvtormipwaoeriengdhtsso
Thheeco1n8tr0ol00gr6o2up,pthmesai.itffeitrebaicredswweereedeuutboantizeedamtitnhefnadolfeWreemkiioofntfhottehse,seuerimngtpgiroduposf.
igh eggproduction. Thecontol the begianingofWeek20ofthe
ersotwbsyawhdic1h76tpipmemcag.gtprreoadtumcetnitognrhoaupddetecsltibinreddswdreameatciucatllayi. zdOnaet
600380
---
-- WildliSfLe TInTtTeArTnaItOiNoAnaLl,,TLtHd.
PPreoejeNctmNubmebear4s5t4-i10o5s
5.
Would reserve
expe inthe
ibviedrsanindcflol mreperosducptiaveignchonddiitvieonnr,wgepialrgthiytcsul.aWrhlyefnemcaolmesp,arteodthoatvehhcioghnereegvraeusp,otfherfet
Wheirlee0h0eapepwaaresnt sriesgtmaenntienlcariedaseefifnemctssonjfvreisnlge vveesrwweeiigghhttfaorntyeo1f.8pbepcmonac.enetraatimonesgrenosutpte,d.
tthiesgcrroeupa.seMweasaontdduolsl aredspoofnsfivienagndliavpeprewaeriegdh1tsbaerereplraetseeniesldiignhtTyabhliegh5e.emenadnbiyvwiaeildgthutsnvfelorr
weightsweprescned in Appendix XI AppendixXv.
Individual offspring iverweighs are prescted1m
Mallrdswere exposed to
concLusion PFOSatdietaryconcentrationsof 0,
15,
62 and
17.6ppm
a,
for
163wweeskeak.s.TNhoectornetartomlegnrtoeulpaatneddmor1t7a.l6iptpiems w.e.reteosbtsgerrovuepdwaearnmyionfstihneecodnocnenttsrtatiioenss tfeosrtead. aNdoioovneratl
csiognncsenorf atooxnic,itayswienrgeenohteendwaatstnhoete1d8wiotrhs6i2gpnpmofait.oxitceisttyctohnactemnatryaboanvse.bAeLetnerela1ted p7tpo mr6aca,metnxtt,
6"T2heprpe mwrae.0teptpcoanrceenttrraetisomnes.nTt eerlaetweedfreecmtesaonnbbooddyywweeilgghhttaIsossamloensg1madlefesmaaldesfenmtahlees 1.i8stnhed
1e7l.a6tepdpemffeac.tsotenstegerdoucpotnhsautmmpaioyn oharvebgecprnordeulcattieodnitonttreeatm1.e3,nt6.2Tohe1r7e.w6eprpemno..aprpaeraenmterteagmroeunpts..
THihsetroepawteorleo5gc0aplaerxeamnitnattieosnmoefnsetlreecltaetdedi.seffuecrtesvoeanleadnya oigfhethrirnecpirdoendcuecotfivteepsatircaumleartreergsrescsiaosnaendd.
I
madaiypboseeitnicsisduemntalitotcreatrmento, frveoamaeedoittsmeaailteicnftfhlcet17ca.o6updopnmotnba.e pgrreoculpu.dedW.hBiastedeuspobnteheerrveasdotnss
ofthissty, the w6a2 ppsm a.
no
bservedefTct
concentration
formllardsexposed
o
PEOS
i
the
cit
for
sx
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|
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600382
-- r Wildlife International, Ltd. E PPreojkecetNNuembbeers45t4-e1s05 "26.
REFERENCES
1 UESuaSbv.divrEionsnvimioenrnotnlEm,P_ernoHtteaoclatriPodrnoAtBgeecnutciyoa,nO:Afgfeinccyo.WfiPdesi1tf9ie8c2i.deanPdrPoegarsatmcae.cWAsaOsrsegshmaiennnstt,DGou0snidilionens,sFImFrRA 2 ASRtmeapenrrdoiadrcidacsnt.ivVSeooclSi1ei1ty.s0f4.oitPhhTieAasvtdiieanpnghiSeap,necdpiAe.sM.at1eA5rpSipaT.lMs St1a9n8d6a.rdSEt10nGd2a.8r6d.PrAoncsaefoorlCooranndsi 3 Me&rCoc.okc. 191. TheMerckVeteinryMasa. Mer&cCok. Rava NJ. 1832p. 4 NaWtaisohnianlgtRone,seDaCr.cNhatCooumnacilA.cad19e9m6y.PrGousi.de13f5porpthe Care and UseofLaboratory Animals S DwuimtmheatCtoaCtWol.. J1o9u5:5.AmAerMuSltilp.lesCoomepa0ri:so1n0s96P1r1o3c1edure for Couparing Several Tresmeas 6 Dounn.nett, G:W. 1964. New TabforlMuletipsle CompawritihsaCoontnrosl Bomeres 20: 482
:
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Pagel
CoorfAnasys
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Contr synLabora GOARare: GUA
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000393
-
38. Wildlife International, Ltd.
Project Number 454-105
Dict and SuAppppleenmdeinxt Flo.rmulations Table 1 `Wildlife Intemational, Ltd. Game Bird Ration'
oREDENTS SoFeiynvoBiCeobmbee,t4%ria PARreRoyVSnpeecZ,kri oprnot EDaGrnromeiCsaoenns MVieamnionrtdiiens+rLigm (below) SeElSo `GLFerm(Fermatco)
PERCENTOB wtrones]s i0io aa2% kx aax3 _Tao0i2oo5
SViniinahnedMlileoPsemwio,ihoentdged 32%of ere, AmSupploPesTo:
pVViieaannx,
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PrBFiotoocnsaicohe
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crude fatand
amaximum of
5%
600399
--------------------------------------------------------------
"39. Wildlife International, Ltd.
Project Number454-105
DiActpPpreepnadris1ix1on
June 1P9r,e2m0i0x0.e NfoomriPnRaOlpSwrereeppraepwraarsaedaostnfiolFoleborwnusa:ry28,2000; Apel7, 2000;May12,2000;and
Cont: Nopremixrequired.
.
Leppmaic 03084 gP+6F099.O7graStion
62ppmai: L0794g60P98F9gOraSti+on
I76ppmais 30839 PROS +60969 gration
ra BasatlnoratiWont arwiansgweeibglhedenddiert.oThsteareedstHsoubbasrttanmicxeiwngabsowweli.ghAepdprionfxoim8attaerleyd1weig0ohfbroa0atitoanwngadsa `sWmaarlolgm?orbtlaewadsertaankdetnhteowcerisghhabnoytawraessorifncsoendstohlrdeaetitoinm.esTwhiethesrattsionufombtwhseamsitxtrianangsbfnoerweldc,foweihteh {btorrinase bnetisontgfhaedomdiexrdinomgtbheoewldle.ndTehre. bTihnedbelrewnadesrrcinsoedtmhweeeerteimnmiesxtwedihspupnrcoonxtiammtienyatoendmriannut raonmd tchonbteonwts,wweiettmheixrendsapbperionxgirmeateulmyed1S0mhiembeowsl.TThheebproemwixwawsapslwaeciegdhoend anHtooba1r0t0m0i.x0egr,sainqdotth,e Placed inappropriately abled plastic bags, reweiangdhsoere,d frozen.
Asnested,the spproprisepremixwsincorporated to the ial dietasflows: Oppmais 2375kgr+1a250ikgliomestnone 1$pmaii 1000gPremix+ 275kgration + 1.250kg limestone 62ppmaic 1000 Premix-+ 275 gration + 1.250 kglimestone 176ppmaic 100gPremix+ 2275kg tion +1250kglimestone "Thedietsweremixed for approxin20amtineulteysin aPateson-TKwienlShlelylBlder
600398
0.
Wildlife International, Ltd.
Project Number 454-105
Appendix IV
"TheAcai ofPFOS in Avisn Dit
.
60039
te Wildlife International, Ltd.
Pre Number 54.105
ApexV
ANALYTICAL METHODS AND RESULTS
pre
TypicalLC/MSOperational Parameters
oT,
eetePrFt ioAy1P00OoiEhFeaooSorcaLoosi tCotrntoemwh hE
ANALYTICAL COLUMN: KetoneBey lun (520mem, i aici) oven TEERATRE. 30
store
I
row:
otto
MOBILE PHASE:
72.0% Methanol :28.0% NANOpureWatercontaining0.1% FormicAcid
INECTIONVOLUME: sou.
rRoEsTENTONTIME Aim6 iyn
,
pRrETENTIONTME Aim26 iyne
rNooswromuAss: mum
|
P INTERT NAL STANDARD pore
600397
----------------------------------------------------
a Wildlife International, Ltd.
Project Number 454-105
Append IV
TaBLE2 CALCULATIONS
ThecomcptrionofPROS ord ttheinstantws determinedusing thefollowingequation: FFOS (8 04..1)st nstrament =ponenntl inert) otro standscon.(mg8.1.1)
Deteminsionf Semple Rs "Theconcentration, xpresedssppma. forcachsample wasdeterminedwin he following
equation:
PFOS(pma..)insump =PFOS(mg 0/1)tnt.vexe te(5vg n)olume (1) itionfictor
DstemLiiminofaQuaintiotatniono0.0fQ) ThemethodLOG,expressed sppm ..ws determinedusinthefollowingequation:
LOQ(5pm ..) = owes sandardconcenation(mg:/1) xoveralldilution factorofmati blank
over inioneeoftikmpl 2Elle(1)lt tr
pont
}
Thepm ..measured nachspeisdividedby th nominalconeeation ofeachsumple (oni ve,pma...Thisratotimes 100s he percentrecovery fthemethodat hteve of foniation.
Recovery PBemRaA.mmeesntweedindssapmplle
|
|
600338
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wl Fl Wl 51 %1 1
1
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21 1
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211 1 228
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tao
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*5=7 :
BRRI
pEEE
3337
BEE 3383
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5
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RES :
BRR
FEIE
935
33EE 333
Ages ress ZEEE mee rao 223%
oo Hoe g 15 os 154
oxo og .853 13 3 owl 156 el noes nose 1= 5 41 vIES Ix oe 8) . zee 5 osm Hoa 1% 2g
82RT 12 4 il
sos 5x3 nor w eee 187 3 GEi la 3 al
BEER E54)
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535 12 ge 81 sed 12a 0 sees 17 ga 3) X65 15905 30 een ysgaaal
12352881 vee [333REGI 299% 1g-ries)
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AppendiVx
PPrriojeecNtNaubmebresr4s5e4-i1o0s5
intensity: 40000 cps 10
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