Document nk50rpgxn7pBe06K4q42MBGg1
M ICROBICS M ICROTOXO TOXICITY TEST
TEST SUBSTANCE
Identity:Perfluorooctanoiaccid,ammonium saft;may alsobe referredto as PFOA ammonium saltA,mmonium perfluorooctanoateP,FO, FC-1 16, FC-1 26, FC-1 69, FC-1 43 oras the major component of FC-1 18. (Octanoicacid,pentadecafluoro-a,mmonium salt, CAS # 3825-26-1)
Remarks: The testsample isFC-1 18. It'psuritywas notsufficiently characterizedt,hough currentinformationindicatesis20% FC-143 in80% water. The 3M productlotnumber was not noted.
The followingsummary appliesto the testsample as a mixture of the testsubstance In water solutionwith Incompletelycharacterized concentrations of Impurities.Data may not accuratelyrelatetoxicity of the testsample with thatof the testsubstance.
METHOD:
Method: MicrobicsMicrotox@"BASIC" Procedure Test type:Staticacute GLP: No Year Completed: 1996 Species: Photobacteriumphosphoreum Analyticalmonitoring:pH and osmoticcondition Exposure period:30-minutes Statisticamlethods: EC50 valuescalculatebdy statisticlailnear regressionprogram providedforMicrotox@ Test organism source: Microbics'Reagent. Test conditions:
Diluent:MilliporMeilli-QTM water Stock and testsolutionpreparation:A primary10 g/Lstocksolution was prepared inMilliporMeilli-QTM water. The pH ofthe stocksolution was 6.0 (no adjustment)and an osmoticadjustmentwas made using200 mg NaCl in10 mL stocksolution.Appearance oftestsolutionswas noted as "clearand soloriess"A.lltestsolutionswere made by proportional dilutions. Exposure vessels: 3 mL Cuveftes. Number of replicates:two Number of concentrations: Four plusa blankcontrol Element basis: Percent lightloss.
RESULTS
NominalconcentratioBnlsa:nkcontro6l2,5,12502,500,and5000mg/L Element value: 5-minuteEC50 = 4460 (4020- 4950) mg/L
15-minuteEC,50= 3360 (3090 - 3641) mg/L 30-minuteEC50= 3150 (2910- 3420) mg/L
Element valuesbased on nominal concentrations.
Remarks: Testingwas conducted on the mixtureofthetestsubstanceas describedinthetestsubstanceremarks fieldT.he valuesreportedapply tothatmixtureand notthetestsubstance.
CONCLUSIONS
The FO-118 30-minuteEC50for Photobacteriumphosphoreum was determinedto be 3150-mg/L witha 95% Confidence Intervaolf2910 to 3420 mg/L.
Submifter:3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,MN, 55133
DATA QUALITY
ReliabilityK:limischranking2. Testingmeets allcriterifaorquality testingb,utlacksanalyticaclonfirmatioonftestsubstanceconcentrations. There isa lackofcharacterizatiofnthetestsample.
REFERENCES
Thisstudywas conductedby the3M EnvironmentalLaboratoryS,t.Paul, Minnesota,Lab Request number Pl626.
OTHER
Last changed: 5/25/00
ENVMONMZ&NTAL TESTSUBSTANCE:
LABORATOR*: MCROTOX
PREP SHEET
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