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,Y * p. . PROPOSAL TO THE NEW YORK STATE HEALTH RESEARCH COUNCIL TITLE: The Environmental Health Implications of Aerobic and Anaerobic Decomposition of Polychlorinated Biphenyls (PCBs) in the Hudson Estuary PRINCIPAL INVESTIGATOR: Dr. Chun C. Lee (SSN 521-52-6704) INSTITUTION: New York University Medical Center Institute of Environmental Medicine Laboratory for Environmental Studies 550 First Avenue New York, New York 10016 DURATION OF PROPOSED WORK: January 1, 1979 through December 31, 1979 TOTAL AMOUNT REQUESTED: $31,080 a. ...... ............. . Chun Chi Lee Research Scientist Institute of Environmental Medicine New York University Medical Center Tuxedo, New York 10987 Rerril Eis'enbud Associate Director Institute "of Environmental Medicine New York University Medical Center Tuxedo, New York 10987 Norton Nelson, Director Institute of Environmental Medicine - New York University Medical Center 550 First Avenue New York, New York 10016 'ThomasJa. - Fitzgerald, Director - Office of Grants Administration and Institutional Studies 550 First Avenue New York, New York 10016 *0N$ 01*660 NEW YORK STATE . HEALTH RESEARCH COUNCIL GRANT APPLICATION PRIORITY AREA 7 LAST NAME _ L?e........ _ 'c .. SECTION I SOCIAL SECURITY __ 521-52-670<_ .. .............. 'WBEfC0MPrETE*lT'B7 PRfNCT>AL'lNV"STTTfAYOR........ .*. T. TltLE'OF PROPOSAr Mie"fenvirningniry.*c"' DEPARTMENT,'service, Laboratory Health'Implications of Aerobic and AnF- OR EQUIVALENT Lab. for Environment* aprobic Decomposition.of^Polychlorinated Studies, Inst, of Environmental f. PRINCIPAL 'IWeSTTgATOR Biphenyls (PCBs) Medicine, NYU Medical Center i'n t"he Hud~ son Estuarrv2. H. MAJOR 'SUBDIVISION......... "~ "A. NAMf (Last, First, Initial) _ Medical Center Lee, Chun C. - I T.~DATES OF'PROPOSYd" PROJECT PERIOD (THIS APPLICATION) . . "mi* ~6if position"............. [__ ^FronJan^l .[VRllirou^hpec^ 31, 1979 . Research Scientist and Adjunct 4. TOTAL DIRECT 5. DIRECT COST'S' Assistant Professor _ _ __ COSTS FOR PERIOD REQUESTED FOR FIRST Y. 'ty MAILING ADDRESS (Street, City, IS ITEM 3 12-MONTH PERIOD P.O. Box 817 State, Zip Code) _5nt080_______ 31/080 ' Tuxedo MY 10987 6.'PERFORMANCE SITE('sT A. Primary Site A.J. Lanza Labs tnt" Ph.D. DECRJEE ~ry~5*r21-S5O2C-I6AL70S4ECURITY No. B. HEW YORK STATS SENATORIAL DISTRICT HUM3ER 26 S.F.` TELEPHONE NUMBER C. NEW YORX STATE 'AC.(91J )3 51j-_4 2 2 9jxr.______________ _ _____ ' ^ASSEMBLY DISTRICT NUMBER 65 7. RESEAROl' EVOLVING HUMAN SUBJECTS " A. X Ho B.___ Yea-Approved:________ C. _ Tea-Pending JKevlev _ Date r. TO BE COMPLETED BY RESPONSIBLE ADMiNISraATlVE AirrHORITY" 'applicant' ORGANIZATIONS (S) P-0. NAME. TITUS, ADDRESS, AND TEUPHOSS HO. institute of Environmental Medicine OF OFFICIAL IN BUSINESS OFFICE WHO ' MTU Medical Center SHOULD ALSO BE NOTIFIED X? AN AWARD IS XADE. 550 1st Ave., New York NY 10016 John N. Ballow, Vice President for Finance IRS f 135562309N _____ TELEPHONENUMBER 212 679-3200 ext, 3611 fyNAME,' TITLE, AND' TELEPHONE NO. OF P-1. Dffiw organizational awpbaujf to OFFICIAL (S) SIGNING FOR APPLICANT RECEIVE CREDIT FOR INSTITUTIONAL GRANT ORGANI2ATIOM Y-A. Fitzgerald, Director (SEE INSTRUCTIONS) 01 Hew Yorlt University Medical Center iy.~CERX'i?ICAfi(W AND ACCEPTANCE, -kb, THE UNDERSIGNED, CERTIFY that THE STATEMENTS HKRELN ARE TRUE AMD COMPLETE TO THE BEST OF OUR KNOWLEDGE AND ACCEPT, AS TO ANT ' GRANT AWARDED, THE OBLIGATION TO COMPLY WITH NTS HEALTH RESEARCH COUNCIL TERMS AND CONDITIONS IN EFFECT AT THE TIME OF TEE AWARD. Tignatukes" (SIGNATURES REQUIRED ON EACH SIGNATURE OF PERSON NAMED IN . ITEM 2 .A. _____ DATE COPY. USE DARK INK. "PER" SIGNATURES HOT ACCEPTABLE.) 's'iGNAEW OrJPEisCN NAMED IN ITEM'S. dais' .. c _ (KOTE: Principal investigator oust also profess and sign professional assurance at the end of reseesch plan) - '. HONS 012661 m4 : Priority Area f ^ 7 . Last Name Lee <* Section I RESEARCH OBJECTIVES Social Security 1 * lliJ JjMl-52-6704 * NAME AND ADDRESS OF APPLICANT ORGANIZATION New York University Medical Center, Inst. of Environmental Medicine 550 First Avenue, Mew York/ New York 10016 name, social security number, official title, and department of all PROFESSIONAL PERSONNEL ENGAGED ON PROJECT, BEGINNING WITH PRINCIPAL INVESTIGATOR ' Chun C. Lee, Ph.D., 521-52-6704, Research Scientist and Adjunct Assistant Professor, Laboratory for Environmental Studies, Institute of Environmental Medicine, NYU Medical Center (914)351-5419 ' Theo. J. Kneip, Ph.D., 031-36-5155, Deputy Director, Laboratory for Environmental Studies, Institute of Environmental Medicine, NYU Medical Center .. '. Joseph O'Connor, Ph.D., 011-34-2794, Assistant Director, Labors- tory for Environmental Studies, Institute of Environmental Medicine, NYU Medical Center . TITLE OF PROJECT, THE ENVIRONMENTAL HEALTH IMPLICATIONS OF AEROBIC . AND ANAEROBIC DECOMPOSITION OF POLYCHLORINATED BIPHENYLS (PCBS) IN THE HUDSON ESTUARY '. I It is known that PCBs recovered from environmental samples (e.g., water, sediments and organisms) are different from commercially produced products by having an increased abundance of isomers of high*chlorine content; isomers of low chlorine content are . readily metabolized by organisms at lower trophic levels, or are otherwise degraded in the environment. It is also known that PCB Isomers containing 50% or more chlorine are less active, biologi cally, than the lower chlorinated isomers, hence those isomers having high chlorine content may be less toxic. Consequently, the health risk from environmental accumulations of PCBs may ' change with time because of differences in the degradation ' rate. This study is proposed to investigate the mode and rates Of PCB degradation (Aroclor 1221, 1016, 1242 and 1254) by ' aquatic and estuarine fungi and anaerobic bacteria, and to assay the relative toxicity of the PCB mixture remaining after micro bial action against some key Hudson River organisms. i HONS 012662 fR(WECT~CpNTR6y _____________________________________ ___ Priority Are* I__?________ _______ ____ __ Last Name_i__ ___________ _____________ Social Security #_^21-52-670_4__ ______ HRC # (Office use only)___________________ Type of Application: Total Direct Costs Requested: ___*._Kev First Tear QS.Q________ _ .___ Renewal of HRC I______ ___ Total ___?;?;______ * Date Suboltted_JJoye.mbgr_ij__l.828 Principal Investigator chun c Lec Ph-D` ______ ____ ____________ ____________ Applicant Institution ew York University Medical_ Center .................... Research Institution Institute of Environmental Medicine, NYU Med^ Center Ira S. Goodman - Assistant Director, Office of Grants Grant Manager:,_____ _Admin-lsiTAtlon. A. Instituti-ona! -Studios--2-12~679-'3200 X2233 name phone Title of Proposal: The -Environmental.Health Implications, of Aerobic and . Anaerobic Decomposition of.Polychlorinated Biphenyls . (PCBs) in the Hudson Estuary . DO HOT WRITE BELOW THIS LIME jgompletcness Validation (1) Proper Signatures W Patent/Copyright Agreement (BKC-23) , . 0> Protactlon of Buman Subject* (HRC-1) _______ _. () Protection of Labortory Animals (Letter) ______ (5) Principal Investigator Assurance (Statement and Signatures) < Progress Report (Renewal) O) Invention Report (HRC-Zl) <8) Annual Invention Statement (HKC-22) Data Received. ________ ______ ____. Date Reviewed_______ ____ ____ HONS 012663 PRIORI!"! AREA #_ 7 ___________ ,v " SICTION. II '' last Same lee __________ ;___ PRIVILEGED COMMUNICATION Social Security #_5^U-52-_6704_ DETAILED BUDGET FOB FIRST 12-MONTfl PERIOD JUfflOJIT REOUSSTED to PERSONNEL TITLE OF POSITION Z OF TIME OR. EFFORT ~ FRINGE SALARY RATS X TOTAL 20.5r"to 8/31/79 ~cu. 3~led.. _Pr inci pa 1. .1 nves tiqator .20%. _3.Jt,_0*C5.auor jTechnigaX Advisor^ 5% ..______ 41486 N.C. fiV afters 9/1/79 _~74 15^4 6 ________ _-0- _ _._Knelp.-- ____TJ3&.________ ____ .TBA. -...... ... . Te.Qh.ai cal Advjspx. _Secreta rj e.lE_ j/flbaraip.ry^Aasi^ ____ 5%_ _Nj.SU-- ___ 2.Q%_ 21407 _jLftsa. J.2...Q0Q, 2,606 _ _-0-" _2j 930 .14160 6 --------------------- ........... fo Subtocal Xiulai (2) CONSULTANT C0STS_ ------- ---------------------------------- ------ _____ ____ __ -r,----- ' (2) Subtotal !__ _n____ _ (3) EQUI?KPIT__VAri ahle--shalcer.___---------------------------------- i 500______ 1.2 2,33 6 *__-.ft-.____ ~l3) Subtotal SL33Q-~~~SS- C*) WPPirathfimi.tia,l5.-reagents, media supplies,.... L. ,,lbell4-Rn4.i)nLbgl.isA^.Qfis- 1.000 _Koutlne_glRSS.vt4.ee _a.nQi_s.olvent__ _________ ___ ________i__, _d_o_o _riiromatogr aj>hic..^uppUe.Sj_sA&5Sj__cfi.l,uiEn_. --------_J 5_o Miscellaneous supplies . (*) . - -- <' i__. ,,25CL . Subtotal 3,000 (5) TRAVEL _______________ ________ 1______________________ 3____ ______ ________ Travel--hn .one_rloinp.stlc_iaaetiiig..anGi____ ______ 4 0J9_ _____ for_travel_io .consul -colleagues. ______ _____ ,,...__ /ininn_.tla ------------ _--------------------- (3) Subtotal XT. .'liinfl... (6) PATIENT COSTS__ * !_ -5CKL l_3t90Q_. S___ .4(10. " . ............... .. " . (6) Subtotal T~| ~_q_ ~ <7) OTHER EXPENSES (DETAIL)JDyrj1M1L.a51.0l_tOtRlJL 1--CJL8J-. i~-.Q7.__ (I) US. DIRECT COSTS. (7) Subtotal T ................ TOTAL Page 3 ' . 4T184 " 8..4.t1.84. 31.080. "ONS 012664 SECTION II BUDGET estimates tor future tears DIRECT COSTS OSLT (Onlt Cents)* Priority Area #___ 7 last Ran*_Lee Social Security # 1.21-5^670.4 . Description First Tear (Same As. Detailed Budget on Page 3 Second Tear (1) personnel Costs, indexing'fringe benefits (2) Consultant Costs (Include fees, travel, ate.) $22,996 -0- (3) Equipment (4) Supplies . (5) Travel (() Patient Costs ' ' (7) Other Expenses . (overhead 15t of total) _____5.0J7....L. __3,.Q.0fi.__ . ____ 40.0___ -0 .4,194____ TOTAT. DIRECT COSTS *31jfi.80-____ $_N.A____ . BTMARRSi Justify all costs for the first year for which the need nay not be obvious. Por future years, justify equipoent costs, as well as any significant change In any Other category. (Use continuation page If needed.) Five hundred dollars is requested for the purchase of a small variable shaker assembly for the gtowth of fungal and bacterial cultures. This piece of equipment Is not available at the Institute. VOTE! Completion of these columns does not obllgste the NTS Health Xeeearch Council to future funding. An application for reneval must be filed each year. Page 4 MOHS 012665 SECT10NII Priority Area l_J Uit San_i,ee __ Social Security I _ 521-52-6704_ OTHER RESEARCH SUPPORT ' list all research support for each Individual Including requests now feeing considered, as well as any proposals being planned, regardless of revclance to this application. Include also current or pending contracts, fellowship awards, research career prograa awards, training grants, regardless of the source of support. Include title of project, funding source, amount of support, and Investigator's time commitment. List grant Bcmber(s) for all current support. - Support for Lee . .. .{ . Current: Ncw york 6tate DEC - Monitoring of PCBs in the Hudson River . June.l, 1978 - May 31, 1979 $44,000. 20 % of time. ` New York University Medical .Center Institutional - The Anaerobic Degradation of PCBs by Aquatic and Estuarine Bacteria. July 1978 - March 1979. $5,000, no salaries. lading: New York State ERDA - Power Plant Simulator Studies December 1, 1978 - November 30, 1979 $90,000. 10% of time u. * -. planned:u. s. NOAA-Sea Grant - Comparative Toxicity of of Environmental PCBs vs. Commercial Preparations of PCBs in Hudson River Pood Pish. Approximately $75,000. . U.S. EPA - A Proposal to Devise Clean Up procedures for Labor atory generated wastes. Approximately $10,000. Page 5 MOHS 012666 ' ( . Priority Area f_^ . BIOGRAPHICAL SKETCH Last Name__ tec . ,. Social Security f _521-52-6704 (Give the following Information for all professional personnel beginning with the Principal Investigator. Use continuation pages and follow the same general format for each person.) ' ' ....... " name ........: 'title BiRTHDATE Chun C. Lee, Ph.D. Research Scientist and (Mo..Day, Tr.) OB/31/39 _ Adjunct_Assist. Prof. __ PLACE OF BIRTH (City, State, Country) Present Nationality (If non- Sex U.S. citizen, indicate kind Male Hong Kong of visa and expiration date) __ Female """ EDUCATION (Begin with baccalaureate training and Include postdoctoral) " . ` .............. -..........""YEAR ' SCIENTIFIC __ ...... INSTITUTION ______DECREE_____ CONPERRED_FI#_........ Colo. State U. Ft. Collins, Colo. B.S. 1962 Eoology l). of Miami Coral Gables, Fla. PH.D. 1969 Marine Science SONORS....... * ........................ ........... ........... fS Achievement Award in Biological Science, Colo. State U. 1962. NDEA Fellow 1962-1965, U.of Miami. _ RUM RESEARCH'INTEREST ....... BOLE IN PROPOSED PROJECT .......... ....... Environmental Pollution Project Director RESEARCHAND/OR PROFESSIONAL EXPERIENCE (Starting with present position, list training and experience relevant to area of project. List all relevant publlcationa.) See Attached Curriculum Vitae r> Page 6A HONS 012667 * * BIOGRAPHICAL SKETCH ' Priority Are* ' Last Name____Lec ...... . , Social Security I _ 521-52-6704_ (Give the following Information for all professional personnel Beginning with the Principal Investigator. Use continuation pages and follow the same general format for each person.) ' '` HAKE : TITLE BIRTHRATE (Mo..Day, Tr.) Theo. J. Kneip, Ph.D. Dep. Dir. Laboratory for 12/20/26 _________ Environmenta1 S tud i e s PLACE OF BIRTH (City, State, Country) Present nationality (If non- Sex " U.S. citizen. Indicate kind _x Male St. Paul, Minnesota, USA of vlsaend expiration date) ___Female EDUCATION (itegln with baccalaureate training and Include postdoctoral) """ ...... .......... ............ ......................... ....................... YEAR "SCIENTIFiC _INST1TUT10W AND LOCATION^_____ JNSBREK,____CWmiffi______ PIBU>____ University of Minnesota University of Minnesota University of Illinois B.S. M.S. Ph.D 1950 1952 1954 Chemistry Chemistry Chemistry foWDRS "* " ............ " ' '* *................. ~ .......... ... Phi Lambda Upsilon, 1950 - Sigma Xi, 1954 KaJOR RESEARCH INTEREST ........... Environmental Pollution ROLE IN PROTOSED PROJECT .. ............. i Technical Advisor ' ' RESEARCH ANl>/OR PROFESSIONAL EXPERIENCE (Starting with present position, list training and experience relevant to area of project. List all relevant publications.) See Attached Curriculum Vitae Page 6B HONS 012668 Priority Area ')____ j. BIOGRAPHICAL SKETCH Last Name Lee , Social Security t 521-52-6704 (Give the following information for all professional personnel beginning with the Principal Investigator. Use continuation pages and follow the ease general format for each person.) NAME Joseph M. O'Connor, Ph.D TITLE Assist. Dir., Laboratory for Environmental Studies BiRTHDATS (Mo.,Day, Yr.) 11/19/44 PLACE OF BIRTH (City, State, Country) Boston, Mass., OSA Present Nationality (If non Sex -s- citizen, indicate kind _X_ Male of visa and expiration dace) ___ Fenale EDUCATION (3eein with baccalaureate training and include oostdcctoral) YEAR SCIENTIFIC INSTITUTION AND LOCATION DEGREE CONFERRED FIELD College of Holy Cross Worcester, Mass. B.S. 1966 Biology State U. of New York at Albany Ph.D. 1971 Ecology/Physiology HpNORS h/ Sigma Xi, Beta Beta Beta Biological Society MAJOR RESEARCH INTEREST Environmental Pollution ROLE III PROPOSED PROJECT Technical Advisor RESEARCH AND/OR PROFESSIONAL EXPERIENCE (Starting with present position, ]isc training and experience relev anc to area of project. List all relevant publications.) See Attached Curriculum Vitae Page 6 C HONS 012669 ' Priority Area 7 , last Name .__Lee RESEARCH PLAN Social Security I ' 621-52-6704 BIBLIOGRAPHY, Provide a detailed bibliography that is relevant to the research project. List any research performed by the principal investi gator (s) named in the application. Use continuation sheets ' lf'jiecessarjr. _ __________________ _______________ ____ __ _ Ahmed, M. end D.D. Focht. (1973). "Degradation of polychlorinated biphenylB by two species of Achromobactej:," Can. 0. Microbiol. 191 47. ......... ... ~ ' Alexander, M. (1965). "Biodegradation: Problems of molecular recalcitrance and microbial fallibility," Adv. Appl. Microbiol. 7* 35. " ' ~ ............ Alexander, M. (1975). "Environmental and microbiological problems arising from recalcitrant molecules," Microbiol. Ecol. 2: 17. Aulerich, R.J., R.K. Ringer, H.L. Seagran, .and H.G. Youatt. (1971). "Effects of feeding coho salmon and other Great Lakes fish, on mink reproduction," Can. J. tool. 49: 611. Aulerich, R. J., R.H. Ringer, and S. Iwamoto. (1973). Reproduc tive failure and mortality in mink fed on Great Lakes Fish," J. Iteprod. Fert. Suppl. 19: 365. Bruckner, J.V., K.L. Khanna, and H.H. Cornish. (1973). "Biological responses of the rat to polychlorinated biphenyls," Toxicol. Appl. Pharmol. 24: 434. " .... Bryant, M.P. (1972). "Commentary on the Hungate technique for culture of anaerobic bacteria," Amer. J. Clin. Nutrition 25: 1324< " - - Cahn, P., J. Foehrenbach, and W. Guggino. (1977). "PCB levels in certain organs of some feral fish from New York State," In: Physiological, responses of marine biota to pollutants. F.J.^Vernberg, A. Calabrese, F. Thurberg, and W.B........ ' Vernberg (eds.). Academic Press, Inc., New Fork, N.Y., p. 51. Choi, P.S.K., H. Nack, and J.E. Flinn. (1974). "Distribution of polychlorinated biphenyls in an aerated biological oxidation water treatment system," Bull. Environ. Contamin. Toxicol. Ill 12. "~ ------- " Corcoran, E.F., and C.C. Lee. (1970). "The distribution of chlorinated hydrocarbon pesticides in the Mississippi River delta and the adjacent Gulf of Mexico," Joint Southeast and Southwest ACS Meeting, New Orleans, La.,~p. 88. *........ ' Duke, T.W., J.I. Lowe, and A.I. Wilson. (1970). "PCB (Aroclor 1254) in the water, sediment, and biota in Escambia Bay, Florida," Bull..Environ. Contamin. Toxicol. 5: 171. HONS 012670 Continuation Sl eet tost NesH!_ Lee Social Security 4 _ 521-52r6704. Ecobichon, D.J. (1976). "Enzymatic and other biochemical res ponses to selected PCBs," Nat'l Conf. on Polychlorinated Biphenyls, Chicago, Illinois', V.""57. .... Folk, R. , D. Gruber, and R. Wullschleger. (1976). "Laboratory study of the release of pesticide and PCB materials to the water column during dredging and disposal operations," Final Rept. of U.S. Army Engineer Contract No. DACW39-74- eoi4?.~' .................. ~..................... . Furukawa, K. and F. Matsumura. (1976). "Microbial metabolism of polychlorinated biphenyl components by AJkaligenes sp.,* J. Agri. Food Chem. 24* 251. ' ......... Gibson, D.T. (1968). "Microbial degradation of aromatic com pounds," Science 161i 1093. Guenzl, W.D. and W.E. Beard. (1967). "Anaerobic biodegradation of DDT to DDD in soil," Science 156: 1116. Halvorson, H., M. Ishague, J. Solomon, and O.W. Grussendorf. (1971). "A biodegradability test for insecticides," Can. . Microbiol. 17: 585. ~ Hague, R. and D. Schmcdding. (1975). "A method of measuring the water solubility of hydrophobic chemicals," Bull. Environ. Contam. and Toxicol.. 14: 13. "" Bile, J.P. (1977). "Polychlorinated biphenyls (PCBs): Unavoid able contaminants in food and food packaging materials: reduction of temporary tolerances," Federal Register 42: 17487. T ............... Hullar, T., R.M. Pleasant, S. Pagano, J. Spagnoli, and W. Stasiuk. (1976). "PCB data in Hudson River fish, sediments, water and wastewater," Report prepared for PCB Task Force, New York State Health Advisory Council. Humphrey, H.E.B., H.A. Price, and M.L. Budd. (1976). "Evaluation of changes of the level of polychlorinated biphenyls (PCBs) in human tissue," Final Rept. of FDA Contract No. 223-73-2209 Bungate, R.E. (1969). "A roll tube method for cultivation of strict anaerobes," In: Methods in Microbiology 3B: 117. O.R. Norris and D.W. RiEbons''Ceils.), Academic Press, Inc., New York, N.Y. Hutsingcr, O., 6. Safe and V. Zitko. (1974). The Chemistry of PCBs, CRC Press, Cleveland, Ohio, p. 197.` '' Page ~8 HONS 012671 Continuation SV-ot InBt Kcno_ i,Gfi Social Security f __ 521-5276704 Hydroscience Inc. (1978). "Estimation of PCB reduction by , remedial action on the Hudson River ecosystem,* Report to the New York Department of Environmental Conservation, pp. 107. Jensen, S. (1966). "Report of a new chemical hazard," New Scientist 32: 612. - -- ' Kaiser, K. and P. Hong. (1974). "Bacterial degradation of polychlorinated biphenyls. Identification of some motabolic products from Aroclor 1242," -Bull. -Environ. Contamin. -Toxicol 111 291. ....... " "............... " *......... Kearney, P.C.' and D.D. Kaufman. (1969). The Degradation .-of Herbicides, Marcel Dekker, Inc., New YorJt, N. Y.* " Kimbrough, R.D. (1976). "Pathological findings associated with chronic experimental exposure to PCBs," Nat'l Conf. on polychlorinated Biphenyls, Chicago, I11inoTs,p.~14. Krull, I.S. (1977). "Recent advances in PCB analysis," In: Residue Reviews 66: 185. F.A. Gunther and J.D. Gunther ' Teds.) ,* Sprihger-Verlag, New York, Inc. Xuratsune, M., T. Yoshimura, J. Hatsuzaka, and A. Yamaguchl. (1972). "Epidemiologic study on Yusho, a poisoning caused by Ingestion of rice oil contaminated with a commercial brand of polychlorinated biphenyls," Environ. Health Perspectives 1: 119. ' ' Kuratsune, M., Y. Masuda, and J. Nagayama. (1976). "Some of the recent findings concerning Yusho," Hat'l Conf. on Polychlorinated Biphenyls, Chicago, IllT, p. 14." ' Lee, C.C. and R. Sansur. (Unpublished data on the growth of an aquatic fungus on Aroclor 1221, 1016, 1242 and 1254.) New York University Medical Center. Liu, D. (1976). "PCB degradation," Mar. pollut. Bull. 7: 83. MacRae, I.C., X. Raghu and E.M. Bautista. (1969). "Anaerobic -degradation of the insecticide lindane by Clostridium sp.," Nature 221: 859. Matthews, H.B. and M. Anderson. (1975). "Effect of chlorination on the distribution and excretion of polychlorinated bi phenyls," Drug. Metab. Dlspos. 3: 371. Matthews, H.B. and M. Anderson. (1976). "PCB chlorination versus PCB distribution and excretion," Nat VI Conf. on Polychlorinated Biphenyls, Chicago, 111.7 p. 50. " HONS 012672 Continuation SI *'ct Lost Wcse_ Leo. Social Security t _521-52-6704 Munson, T.O., H.D. Palmer, and S.M. Forns. (1976). "Transport of chlorinated hydrocarbons in the Upper Chesapeake Bay," Mat'.l Conf. on Polychlorinated Biphenyls, Chicago, 111., p; 2187" ~ ......: ...........* ......... * Nadeau, R.J. and R.A. Davis. (1976). "Polychlorinated Biphenyls in the Hudson River (Hudson Falls-Fort Edward), New York State," Bull. Environ. Contamin. Toxicol. 16: 436. Ncbeker, A.V. and F.A. Fugles. (1974). "Effect of polyohlorinated biphenyls on survival and reproduction of Daphnia, Gammarus, Tanytarsus," Trans. Amer. Fish. Soc. 722.......... New York State Dept, of Environmental Conservation. (1976.). "In the matter of alleged violations of Sections 17-0501, 17-0511 and 11-0503 of the Environmental Conservation Law of the State of New York by General Electric Company," Interim Opinion and Order File No. 2833. 'Nimroo, D.R., D.J. Hansen, J.A. Couch, N.R. Cooley, P.R. Parrish, and J.I. Lowe. (1975). "Toxicology of Aroclor 1254 and its physiological activity in several estuarine organisms," Arch. Environ. Contamin. Toxicol. 3i 22. Nisbet, I.C.T. and A.F. Sarofim. (1972). "Rates and routes of transport of PCBs in the environment," Environ. Health Perspectives 1: 21. ................. "" . Norback, D.H. and J.R. Allen. (1972). "Chlorinated aromatic hydro carbon induced modifications of the hepatic endoplasmic reticulum: concentration membrane arrays," Environ. Health Perspectives 1: 137. ............ " - - O'Connor, J.M., L. Peters, and R. Califano. (Unpublished data on the uptake on Aroclor 1254 from Hudson River water and Hudson River forage by Morons saxatllis.) New York University Medical Center.'"' * ' Raghu, K. and I.C. MacRae. (1966). "Biodegradation of the gamma isomer of benzene hexachloride in submerged soils," Science 154: 263. ~ Rlsebrough, R.W. (1969). "Chlorinated hydrocarbons in marine cosystems," In: Chemical. Fallout. M.H. Miller and G.G. Berg (eds.), C.C."Thomas, Springfield, 111., p. 5. Risebrough, R.N. and B.H. de Lappe. (1972). "Accumulation of polychlorinated biphenyls in ecosystems," Environ. Health Perspectives 1: 39. '~ ~" Roach, J.A.G. and I.H. Pomerantz. (1974). "The finding of chlorinated dibenzofurans in Aroclor PCBs of nt . HONS 012673 Continuation $1 vet L*ct Social Security # . _ 521-52-6704 manufacture,'" presented at 85th Annual Meeting Assoc. Official Analytical Chemists, 'paper~ No. 53", Washington, D.C. October Sayler, G.S., M. Shon and K.R. Colwell. (1977). "Growth of an estuarine Pseudomonas sp. on polychlorinated biphenyl," J. Mi crobi al col~.' 3: * 2"4l. Sayler, G.S., R. Thomas and R.R. Colwell. (1978). "Polychlori nated biphenyl (PCB) degrading bacteria and PCB in estuarine and marine environments," Estuarine and Coastal Marine Science 6: 553. - - - - - - - Stalling, D. 'and 3. Buck ins. (1973). "Reverse phase thin layer chromatography of some Aroclors, Halowaxes, and pesticides," J.A.O.A.C.. 56s 367. ' Stalling, D. and F.L. Mayer. (1972). "Toxicities of PCBs to fish and environmental residues," Environ. Health Per spectives jl s 159. . ~ ............ ' United States Department of Health, Education and Welfare. (1977). "NIOSH criteria for a recommended standard___occupational exposure to polychlorinated biphenyls (PCBs)," DHEW (NIOSH) Publ. No. 77-225. -- Vos, J.G. (1972). "Toxicology of PCBs for mammals and birds," Environ. Health Perspectives It 105. Vos, 3.G., 3.H. Xoeman, H.L. Van der Mass, M.L. Ten Noever de Brauw, and R.H. de Vos. (1970). "Identification and toxi cological evaluation of chlorinated dibenzofuran and chlori- . nated naphthalene in two commercial polychlorinated bi phenyls," Food Cosmet. Toxicol. 8: 625. . Walker, C.R. (1976). "The occurrence of PCBs in the national fish and wildlife monitoring program," Nat'l Conf. on Polychlorinated Biphenyls, Chicago, 111., p. 161. " ..................... " Wieso, C.S.and D.A. Griffin. (1978). "The solubility of Aroclor 1254 in seawater," Bull. Environ. Contamin. and Toxicol. 19: 403. " ~ ....... " Wong, P. and K. Kaiser. (1975). "Bacterial degradation of polychlorinated biphenyls II. Rate studies," Bull. Environ. Contamin. and Toxicol. 13: 249. ~ HONS 012674 Page ii Continuation Sl eet Loot Ncnc Lee Social Security # _ 521-52-670^ A. INTRODUCTION I. Objective: This research will investigate the rates at which aerobic or anaerobic microorganisms degrade PCBs in rivers, estuaries, or landfills. The Investigation is designed also to test the hypothesis that the toxicity of PCB residues is gradually lessened in situ by the fact that the lower chlorinated isomers are more rapidly degraded than the higher chlorinated isomers which are known tojbe less. 2. Background: Polychlorinated biphenyls (PCBs) are i the most persistent contaminants man has introduced to the environment. These compounds are a versatile group of industrial chemicals that have been widely used for more than 40 years. Two-hundred-nine possible isomers of chlori nated biphenyls exist., and each industrial PCB preparation may contain ten or more isomers, as well as impurities, such as polychlorinated bibenzofurans (PCDFs), polychlorinated dibenzodioxans (PCDDs) and other unidentified products (Vos et al., 1970; Roach and Pomerantz, 1974; Hutzinger et al., 1974). These products have been released into the nation's fresh and coastal waters by deliberately discarding PCBs or PCB-laden products into landfills and dumps, or as effluents into rivers (Nisbet and Sarofim, 1972). A small amount 16 lost into the air from evaporation from plasticized products or from incomplete burning of substances containing PCBs. Page 12 HONS 01267S Continuation Sl-t'CC bOGt Social Security i _521-52T6704 As with the chlorinated pesticides the PCBs are relatively insoluble in water and are highly persistent in the environ ment (Risebrough and de Lappe, 1972). These compounds are hot easily metabolized by enzyme systems presently found in nature (Choi et al., 1974; Alexander, 1975) and tend to accumulate in fatty tissues and oils .of animals (Risebrough amd de Lappe, 1972). Because of their low water BOlubility, high specific gravity and high adsorbability, PCBs accumulate in anaerobic or reducing sediments of rivers, estuaries and the coastal cone. (Duke et al., 1970; Fulk et al., 1976; Munson et al., 1976). . ' Although PCBs were first detected as environmental con taminants in 1966 (Jensen, 1966), their potential impact as ' a health hazard was not realized until 1969, after the discovery and description in Japan of Yusho, a human disease caused by consumption of PCB-contaminated rice oil (Kuratsune et al., 1972). Since then, PCBs have been implicated in environmental and occupational diseases ranging from chloracne And liver damage to reproductive failure. They may also act as carcinogens or co-carcinogens (Norback and Allen, 1972; Kuratsune et al., 1972, 1976; Kimbrough, 1976; U.S. Department Of Health, Education and Welfare, 1978). . Laboratory studies with industrial preparations of PCBs (e.g., Aroclor 1242, 1254 and others), and with pure Isomers of chlorinated biphenyls have shown PCBs to be highly toxic ~...... ......... ' Page 13 HONS 012676 0 i Continuation SI -et Inst Ner.e_ i,Gr Social Security I . _ 521-52- 6701 to many aquatic and terrestrial organisms (Bruckner et al., 1973; Crant and Phillips, 1974; Stalling and Mayer, 1972; Ncbeker and Pugles, 1974; Nimmo et al., 1975; Vos, 1972; Walker, 1976). Those organisms which are not immediately affected by PCBs may accumulate them to high concentrations, thereby Increasing the exposure of other organisms through food chain transport (Risebrough, 1969; Cahn et al., 1977; O'Connor et al., unpublished data). " It has been shown that the toxicity of a given PCB - depends upon the degree of chlorination and its isomeric form. Chlorinated biphenyls that have a low percentage of chlorination tend to have a high acute toxicity compared to chlorinated biphenyls with a greater percentage of chlorina tion (Matthews and Anderson, 1975). The results from compara tive studies of chronic exposures of animal6 to specific isomers are confusing and inconclusive, but 'the exposure of test animals to subacute levels of individual PCB preparations (e.g., Aroclor 1242, 1248, 1254, and 1260) resulted in varying degrees of liver dysfunction, which was inversely related to the chlorine content of the Aroclor (U.S. HEW, 1978). Lower homologs are also usually more soluble in water than the higher ones (Haque and Schmedding, 1975), and - are therefore'more available to, and more rapidly absorbed and metabolised by, aquatic organisms. PCBs which can be metabolized and excreted may introduce toxic metabolites to the environment, while the forms of PCB that are not readily Page 14 MOMS 012677 Social Security '? 521-52-C7.TC metabolized have an extremely long biological half-life (Matthews and Anderson, 1976). It has been shown also that isomers having two adjacent unsubstituted carbons in the biphenyl ring are more readily metabolized than ones without the two adjacent unsubstituted carbons (Ecobichcn, 1976). The net effect of the above is that different PCBs have different toxicities and different rates of bioaccumulation and persistence. The evidence in general establishes that PCBs with five or more chlorine atoms are less biodegradable and are accumulated to higher levels in organisms and persist longer in the environment. PCBs from aquatic environ mental samples, such as fish, show on analysis a pattern of peaks approaching that of PCB mixtures with more than 50% chlorination, while the major input of PCBs into the environ ment was PCB mixtures containing 42% or less chlorine (Matthews and Anderson, 1976). Highly chlorinated Aroclors, those containing 50% or more chlorine, comprised approximately 30% of sales prior to 1971; this figure was expected to decrease to 22% after 1971 (Nisbet and Sarofim, 1972). This lends support to the laboratory results described above, that the rate of metabolic attack on PCBs decreases with increasing chlorination. It is therefore possible that PCB residues found in natural waters and sediments and in aquatic organisms which concentrate PCBs via long food chains may not have originated entirely from one specific industrial preparation such as Aroclor 1242, 1016 or 1254. Instead, they may Page 15 MOMS 01267B < * '/ if > Aro.i -i- Continuation Si .:t Lour f,00 *' * Social Security tf 52-670 J represent only the higher homologs of several PCB preparations, the lower homologs of which were metabolized or otherwise degraded along the food chain. The consumption of fish contaminated with PCBs from polluted lakes and streams appears to be the major source of PCB exposure for man and animals. However, there is no record of clinical damage to humans from such consumption ' . % (Humphrey et al., 1976; Hile, 1977), even though the amount Of PCBs ingested was high compared to people who ate no - fish. Thi6 being the case, it becomes unclear whether this lack of adverse health effects from the consumption of fish contaminated with PCBs from the environment is due to the low level Of exposure or because the PCBs in the fish are primarily isomers that have a low-grade toxicity; if so, the more active, more toxic isomers may have been degraded in the environment to less toxic forms. . ' The persistence of some chlorinated pesticides and hydrocarbons and the apparent stability of some of these compounds in the environment fostered the idea of molecular recalcitrance (Alexander, 196S, 1975). As a result, the biodegradation of PCBs has received little attention and few data -exist on the rate at which PCBs are being degraded In nature. However, It has been concluded by many investigators from laboratory studies that PCB isomers containing four or fewer chlorine atoms are degraded rapidly in the environment by microbial activity. Sayler et al. (1978) showed that PCB HONS 012679 Ccntinii.uJ.on l! ut i J-.i u> Aro.i . / hint Social Security # _ 521 -52-C7(W _ resistant, or degrading, bacteria were ubiquitously distributed nd indicated the potential for PCB degradation by natural microbial flora in estuarine and marine environments. Available reports on PCB metabolism by microorganisms have dealt exclusively with aerobic bacterial systems (Ahmed and Focht, 1973, `Kaiser and Wong, 1974;'Wong and Kaiser, 1975; Furukawa and Matsumura, 1976; Liu, 1976, 6ayler et^ al., 1977, 1978). Published works which Indicate that chlorinated pesticides and other aromatic compounds are degraded under anaerobic conditions (Raghu and MacRae, 1966; Guenrl and Beard, 1967; MacRae et al., 1969; Focht and Ahmed, 1970) suggest that the lower PCB isomers in sediments may be degraded primarily by anaerobic bacteria.' ' Work begun in our laboratory with an aquatic fungus (Lee and Sansur, unpublished data), isolated from Hudson River water and grown in Aroclor 1254, together with the works of Sayler et al. (1978) and Kearney and Kaufman (1969) indicate that fungi may also be actively involved in the PCB degradation process. . 3. Rationales PCB contamination in the Hudson River is probably the most extensive in the nation. There are an estimated 450,000 to 600,000 pounds of PCBs in the bottom sediments of the upper Hudson River between Fort Edward and Troy, New York. Although no reliable estimates of PCB content in the bottom deposits of the lower Hudson from Troy to Hew York City are available, PCB content is expected to hONS 012680 -i 11 */i 11.) Arc.j * / CoiU.inn.Ttion &l ut I-u;t InT/.n h'\r- Social Security # 521-32-071) -1 be substantial. The major portion is adsorbed to particles deposited in anaerobic or reducing sediments near the point of PCB discharge. Even without further discharge, these PCBs will remain for some years and will continue to contami nate river biota unless the PCBs are degraded or are removed by water currents, dredging or by bioaccumulation in aquatic organisms. Thus far, degradation studies have centered on aerobic bacterial activity, whose results have little appli cation to the problem at hand, because most of the PCBs are sequestered in anaerobic or reducing sediments. Until now, the role Of fungi in PCB degradation has not been considered at all. This 6tudy is proposed to complement the existing work on PCB degradation to achieve a more complete understand ing about the fate of these PCB deposits. As man is the ultimate.consumer after eating contaminated fish, and in light of the uncertainties described above regarding the differential toxicities of different PCB preparations, it is our intent, also, to examine the toxicity of the PCB mixture remaining after microbial action on key Budson River organisms relative to the documented toxicities of non-degraded PCB mixtures. This information will make it possible to predict the future public health and ecological significance of the PCB deposits. MOWS 012681 CnnUnu.Uion : t tout Ncsu! Soci.il Security f _.521-52Tf,7nj B. SPECIFIC ATMS - 1. To isolate and cultivate from Hudson River sediments anaerobic bacteria and fungi which are able to grow in the presence of PCBs (Aroclor 1242, 1016 and 1254) and to investigate their ability to degrade them. Initially, this will be done with mixed-cultures; .studies will be done with pure cultures as they become available. * v Aerobic bacteria will not be included in this work, as these organisms are being studied by other investigators. 2. To determine the rate of PCB degradation by anaerobic bacteria and fungi thus isolated and to characterize the resulting metabolites and residues. 3. To examine the relative toxicity of the PCB mixture remaining after microbial action against non-degraded PCB mixtures on some key Hudson River organisms such as Gammarus, Neomysls and striped bass larvae. C. METHODS 1. procedures for Specific Aim 1: Sediments for the isolation of anaerobic bacteria and fungi will be collected from at least two sites in the Hudson River known to be contaminated with PCBs, one in fresh water and the other in the saline portion of.the river, with a sediment grab lowered from a boat. Immediately upon collection, the sediment will be transferred to duplicate sets of glass bottles which will be almost filled with filtered river water and sealed with serum caps. One set will be gassed with compressed nitrogen HONS 012682 CoiUlnu.itl.oti S* et '. Jjict i,,,r Soclol Security I _ 521-52-0701 to maintain reducing conditions necessary for anaerobes; the other will be maintained aerobic for fungal isolates. Both sets of samples will be returned to the laboratory for subsequent isolation of PCB degraders by enrichment culture. Enrichment cultures for anaerobic bacteria will be set up by adding 1-2 g of sediments to a .250 ml flask containing 100 ml of an organic medium: glucose* 0.5 g; peptone, 0.5 g; XHjPOj, 0.5 g; distilled water or water of appropriate salinity, 1 1 (pH 7.0). Techniques for anaerobic cultures as described by Bryant (1972) will be strictly followed. Cultures will be incubated for 3 days at 26C; intermittently, culture flasks will be shaken by hand. Isolation will be carried out in a second flask which contains 100 ml of a mineral salt solution: (NH^jSO^, 1 g; KHjPO^, 0.2 g; JCjHOP^, 1.6 g; MgS04*7H20, 0.2 g; NaCl, 0.1 g; FeSC>4*7H20, 0.01 g; CaCl2*2H20, 0.02 g (pH 7.5) containing 0.05V of the appropriate PCB. Following standard anaerobic techniques l.ml of the enrichment culture will be transferred to thi6 second medium and incubated as before until substantial growth is observed. Such cultures will be maintained in duplicate or triplicate. One series will be incubated in the light and one in the dark to enable the growth of phototrophic bacteria as well as strict chetnotrophs and heterotrophs. " Enrichment cultures for fungi will be started in Sabouraud dextrose agar (Difco) and incubated under aerobic conditions. After initial growth, 1 ml of the enrichment culture will be HONS 012683 SocI.il S*;cu;[tv i transferred to the PCB-mineral salts medium (described above) but amended with 1 ml of a trace elements solution per liter. The trace elements solution contains 0.1 9 HjBOj, 0.05 9 each of CuSO^-5 HjO, MnS04*H20, ZnS047 H^O, Na2Mo04, and CoC12*6 HjO per liter. After incubation for predetermined time intervals (4 days to 1 month), aliquots of the Incubated samples will be transferred to separatory funnels-and extracted with hexane. Gas chromatograms will be run with 1-5 pi aliquots of the hexane extracts. Initially,- only the disappearance of certain PCB isomers from the mixed culture systems will be measured. If the PCBs disappear or decrease in quantity, degradation products will be looked for, perhaps using thinlayer chromatographic techniques and with 1<C-labelied PCBs. Particular emphasis will be placed upon the water-soluble products. These will be isolated, after hexane extraction, by column chromatography on ion-cxchange columns and resolved by thin-layer chromatography. Mixed cultures will be purified by plating onto mineral salts agar plates containing 0.05t PCBs. : 2. Proceduresfor SpecificAim J2: Once the respective microbial systems capable of growth on PCBs are obtained, the kinetics Of PCB degradation will be followed by analyzing the PCBs in the growth medium at different time intervals. The cells wi)] be harvested by centrifugation and washed twice with 0.5 M phosphate buffer (pH 7.0). The washed cells will be 21 m:iiwihj<iw.v;i it * *:l l-Hit I.V:.;.: Social Security t ...521-52-670J. injected into culture tubes containing the mineral salts medium and PCB. The procedures employed will be similar to those described by Halvorson et al. (1971) who used resting suspensions of bacteria harvested from a sewage lagoon to examine the degradation of mixtures of pesticides. Comparisons will be made of gas chromatograms of PCBs before and after incubation with test organisms over the predetermined time interval. Metabolites and residual PCBs will be characterized by the use of column and thin-layer chromatographic techniques (Stalling and Huckins, 1973; Furukawa and Ma.tsumura, 1976). 3, Procedures _for Spec!fic Aim 3: PCB residues and residual PCB isomers from microbial activity on the original Aroclor will be recovered from thin-layer chromatograms by scraping the respective spots off the plates and dissolving in acetone. Their toxic effects, if any, will be assessed on the physiology and population dynamics of some key Hudson River organisms (Gammarus, Ncomysis and striped bass and other fish larvae) relative to the non-altered Aroclor. These organisms represent important fish food items and at times comprise the majority of zooplanktonic or benthic species in various portions of the Hudson River, and are routinely cultured in our laboratory. Cultures of each species will be established and exposed to varying levels of PCBs, PCB residues and metabolites in. static systems. Biological effects of PCB exposure will be estimated from several critical parameters: HONS 012685 22 Continuation IV ut i 11 '.i 11 > At n.i . L-'lUt 1.0! Iji'o Social Security f _ 52l-S2-G?(M a. rate of individual growth , b. rate of respiration c. rate of production of young d. limits of tolerance to selected stresses: e.g., chlorine exposure, osmotic stress, thermal stress All of the above parameters will be estimated for cultures receiving PCB stress, as well as those cultured in the absence of PCBs. The data gathered here will be analyzed following basic toxicological approaches to threshold sensi tivity of organisms to toxicants and will be subjected to standard parametric and nonparametric statistical tests. 4. The research tasks will be performed in the following sequence, assuming a beginning data of January 1, 1979: January to March: Sample collection and culture isolation March to July: Degradation experimentation and analysis July to October: Toxicity experimentation November and December: Report preparation D. SIGNIFICANCE - ' In light of our present knowledge about the toxicological properties of certain PCB isomers on living organisms and ' the knowledge that there might exist different rates of degradation for each of the 209 PCB isomers, it is expected that the health risks from PCB accumulations may change with time. This study should provide data for the assessment of long term health risks from PCBs and their residues in the aquatic environment.' . HONS 012686 * i I'.r Ily AriM ; 1> CciilJnu.uIon SI- ut Lnct Social Security 9 - 521-52-f,7(M E. FACII.1TIES_AVAII.ABLE . . *. The proposed research will be carried out using the facilities of the analytical chemistry and aquatic ecology sections of the Laboratory for Environmental Studies. The proposed research will be carried out in the culture and analytical facilities at the A.J. Lanza Laboratory, Tuxedo, Mew York, and at the Hudson River field station maintained t .I by the Medical Center at Buchanan, H.Y. NYU boats equipped for collection of samples and certain field analytical procedures are berthed at Stony Point, N.Y. and operate throughout the open-water months. Dock facilities are available at the Buchanan wet-lab, but only on a temporary basis. Equipment available for this project includes apparatus fort Extraction/concentration of organics . Frecze-dry techniques ' Scintillation spectrometry (low level - beta) Gel electrophoresis ' Thin-layer chromatography Gas-liquid chromatography High-pressure liquid chromatography Respiration studies - Hater quality analysis '' Autoradiography . . HONS 012687 Continuation SI "Ct Last Social Security I 521-52-G7(M Culture facilities and equipment for aerobic and anaerobic microorganisms ' '' Culture of aquatic organisms Maintenance of aquatic organisms '. ' Computer data analysis F. PRJNCJPAT. IHYESTIGATOR ASSURANCE "The undersigned agrees to 'accept responsibility for' the scientific and technical conduct of the research project and for provision of required progress reports if a grant is awarded as a result of this application." Date Ftihc ipa 1'inves tigator HONS 012688 NEW TORE STATE HEALTH RESEARCH COUNCIL FROTECTION OF HUMAN SUBJECTS Assurance/Certlficatlon/Declaratlon (Fur(vanc to Federal and State Lav) ' V . Lee 521-52-6704 Q Crant Q Fdlow STATEMENT OFFOLICT: Safeguarding the rights and welfare of subjects at risk in activities supported under grants and contract from NYSHKC is primarily the responsibility of the instlt tipn Which receives or is accountable to KTSHRC for the funds avarded for the support of the activity. In order to provide for the adequate discharge of this institutional respooslblllt it is the policy of JfrSERC that no activity Involving human subjects to be supported by HTSH> grants or contracts shall be undertaken unless the Institutional Review Board has reviewed an approved such activity, and the institution has submitted to NTSHRC a certification of such review and approval, in accordance with the requirements of Chapter *50, NTS Laws of 1975. 1. TITLE OF BR0P0SAL OR ACTIVITYThe Environmental Health ^Implications of Aerobic . and Anaerobic Decomposition of Polychlorinated Biphenyls (PCBs) ---------------------in-th Hudson-Est-oar-y.--------------------------------i...,,----------------------------------.... 2. Principal Investig'ator/Activity Director/Fellow 1 Chun C. L6e, Ph.D. 2. Declaration that Human Subjects Either Would or Would Not Be Involved tC|A. Mo individuals who Bight be considered human subjects, including those froa whom B** organs, tissues, fluids, or other aaterlals would be derived, or who could be . identified by personal data, would be Involved in the proposed activity. (If no human subjects, would .be__inyolyed, check_tMs bpx_snd_p_rpcee_dj:q_Itea_5. Proposals c determined by the Agnecy to involve bunaa subjects will be returned.) Human subjects would be Involved in the proposed activity as either: Q None of the following, or including: 3 Minors, j Fetuses, Q Abortuses, Ll Pregnant Women, Qj Prisoners, 3 Mentally Retarded, 3 Mentally Disabled. Under Section 7t Cooperating Institutions, on reverse of this form, give naoe of Institution sod name end addresa of offlcial(s) authorizing access to any subjects In facilities not under direct control of the applicant or offering institution. *. Declaration of Assurance Status/Certification of Review DA. Shis institution has not previously filed an assurance and assurance Implementing procedures for the protection of human subjects with the Council that applies to this application or activity. Assurance is hereby given that this institution will comply vith requirements of Chapter 450, HIS Laws of 1975, that It has established an institutional review board for the protection of human subjects and, when requested, will submit documentation and certification of such reviews and procedurts as Bay be required for implementation of this assurance for the proposed project or activity. . 1 |g. This institution has an approved general assurance or an active special assurance for this ongoing activity, on file with the Council. The signers certify that all activities in this application proposing to Involve human subjects have been reviewed `and approved by this Institution's institutional review board in a convened meeting ' on the date of ______________ _______ THE INSTITUTIONAL REVIEW BOARD HAS DETERMINED,AND THE INSTITUTIONAL OFFICIAL SIGNING BEIOW 3CONCUSS THAT: Either Q] human subjects will not be at risk,or Human Subjects will be at ri '5. Name and Address of Institution *. TitLfVf insVituii'on Official ~'. ______ ______________ ____________ _________ ________ ______ ______ ____ .____ phone: Signature of Institutional Official Dace MONS 012689 Not Applicable^ 521-52-6704 LRC - 21 ` Title of invention Grant or Contract Under Which Developed Identifier ' Date of Report Inventor1a Name(s) Official Titie(s) Business Address ~~ Facility Where Invention Made Name of Unit or Building Address DESCRIBE INVENTION IN DETAIL FOLLOWING OUTLINE BELOW. ATTACH MAPS, SKETCHES, PRINTS, PHOTOS TO DESCRIPTION. A. Central Purpose - State In general terms the purpose and object of the Invention B. Background - Describe prior art (Identify by patent number or journal citation) C. Description and operation * 1. Describe completely the construction of invention using reference characters to identify components in attached illustrations. 2. Describe one (1) complete operational cycle. ' 3. If invention relates to a synthesis or identification of a new composition of siatter, describe the product in structural form, if possible, and the process of staking it. Include all available information regarding physical character istics and all test data evidencing utility. __ DESCRIBE COMMERCIAL' POSSIBILITIES (INCLUDE' QUANTfTT AND PRICE RANGE IK AVAILABLE).............. STATE REASONS FOR APPLYING FOR PATENT RATE PLANS TO PUBLISH "NAME'OF PUBLICATION, ATTACH'OUTLINE OF ARTiCLEV 'lF ALREADY PUBLISHED, INCLUDE JOURNAL CITATION OR ATTACH REPRINT. DESCRIBE' AND-CIVE"DATE'(sf OF ANY' SALE OR PUBLIC USE'OF INVENTIoiT (SPECIFY IF'dSE WAS OPERATIONAL OR FOR TESTING PURPOSES) C0N1:R1 BUTTONS TO THE INVENTION BY: ~!J<AOTEE ". JOTSHRCOUNCILOTHER (Identify) PERSONNEL (No. of Personnel) ____ _______ ____ ..._________ _ ...... . . ___ MATERIALS (List Type and Value) ___ ________________ ____ ___________ _______________ FINANCIAL CONTRIBUTION ......... ...................................................... ............. ................ EXECUTION OF DISCLOSURE...... .. ' ~ " " - .......... ........... I,_____ __ _ _____, state the above information with regard to the invention of name of lnventer title of invention the invention. is a true and correct disclosure of data pertinent CD........................ Technical Witness )_ .. Signed Date HONS 012690 7. Cooperating Institutions - Additional Reporting Requirement Use Following Report Format for Each Institution Other Than Crantee or Contracting Institution with Responsibility for Human Subjects Participating in this Activity: (Attach additional report sheets as necessary) INSTITUTIONAL AUTHORIZATION FOR ACCESS TO SUBJECTS . Subjects: Status (Wards, Residents, Employees, Patients, Etc.)__ Number ___________ Age Range__________________________________ Title _________________________ ____________________ _______ Phone Name and Address of Cooperating Institution___ ___________ _____ Official Signature _ HONS 012691 NEW TORX STATS HEALTH RESEARCH COUNCIL PATENT AND COPYRIGHT AGREEMENT The undersigned Applicant, In consideration of a grant/contract awarded by the Hev Tork State Health Research Council, does hereby agree as follows: ' (a) To keep the Council Informed of any potentially patentable device or techinqu conceived or developed by the undersigned. In whole or In part, Individually or with others. -. (b) that a patentable device or techlnque conceived or developed In the course or working on progress or projects funded by the . Council shall be the property of the Council. . (c) That royalties, or any other remuneration received by the ' :applicant will be promptly remitted to the Council and redistributed to the applicant and the Inventor (a) persuant to Council policy. (d) Upon Instruction of the Council, to apply for a patent on any such patentable device or technique and assign to the Council or . Its nominee all my right, title and Interest in such patentable device and execute, acknowledge end deliver to the Council any instruments necessary or required by the Council to effectuate . such application, assignment of this agreement. . . (e) To conform with and adhere to the patent and copyright policy of the Council, a copy of which has been received by the undersigned, as the same may from time to time be amended. (f) To give appropriate credit to the Council In any publication or other form of disclosure or at the request of the Council to ' provide an appropriate disclaimer to the effect that the publication or disclosure does not represent the official position of the Council. . (g) A description of any invention, whether made or conceived by employees or agents of the undersigned prior to funding by tbe Council whether patented or not, la appended hereto and such Inventions shall be excluded from thle agreement. I (we) represent that the absence of any Invention In the attached schedule shell' Indicate that 1 (we) own no such invention at the time of signing this agreement. HONS 012*92 (Signature)' '$*> w*r-7** APPENDIX: NYU Organism Culture ' The New York University Medical Center presently maintains in culture several aquatic species, including protozoa, algae, zooplankton, benthic biota, fi6h, and fish larvae. The species of algae in culture include: Chlprclla, Nannochloris, Chiamydomonas, Melosira, Synedra, Anacystis, and others. Protozoan species include Strombidium, Euplotes, Uronychia, and Paramecium. Many different types of microzooplankton have been sialntained at the Medical Center. Included among them are: Mcsocyclops edax, Eurytemora affinis, Acartia clausi, Daphnia magna, and Cyclops 'Jsicusgidatus. Ostracods are presently being cultured in the laboratory for a study of breeding behavior. Benthic biota maintained at the Laboratory include Carcinus maenas, Bithrooanopeus harrisi, and various species of gastropods. The crabs have been used in a series of PCB uptake studies, as well as in studies of respiration' as affected by hydrogen sulfide and low oxygen tension. ' . Several macrozooplankters are maintained at the Laboratory at all times. These include Gammarus daiberi, B. tlgrinus, G. fasciatus, Neomysis americana, and the larvae of Chaoborus punctipennis. The three species of Gammarus have been maintained for more than four years in aquarium culture, and in incubators at a variety of different temperatures. (torone saxat1,1 is, Cyprinus cerpio, rundulus heteroclitus, and Micrqgadus tomcod are examples of fishes and fish larvae which have been raised and maintained in our laboratory facility. Striped bass and tomcod have been spawne.d and raised in NYU facilities each year since 1975. These laboratory-raised organisms have been used successfully in studies of temperature tolerance, chlorine toxicity, behaviour, and growth. HONS 012693 The following references provide examples of studies conducted by the 'NYU staff using organisms produced at, or maintained in, NYU culture facilities: ' ' . 1. THESES . 'Eichorn, K.L., 1977. the response of striped bass (Morone saxatilis) to heated and chlorinated power plant effluent. r.s. Thesis, Biolgoy Dept. New York University. Cinn, T.C., 1977. An ecological investigation of Hudson River macrorooplankton in the vicinity of a nuclear power plant. . Ph. D. Diss., Biology Dept., New York University. Jinks, S.M., 1975. An investigation of the factors influencing radiocesium concentrations of fish inhabiting,natural aquatic systems. Ph. D. Diss. Biolggy Dept., New York University. . - i Paschoa, A.S.,'1975. The natural radiation dose from radium to Gammarus in the Hudson River. Ph.D. Diss. Sch. Eng. and Science, New York University. Pojc, G.V., 1977. Growth and reproduction of Gammarus tigrinus Sexton, 1939. M.S,'Thesis., Biology Dept. New YorJt " " University. ' . - . .- 2. PUBLICATIONS Beck, A.P.-, G. Poje; and W. Waller.,1975. A laboratory study on the effects Of exposure of some entrainable Hudson River biota to hydrostatic pressure regimes calculated for the proposed Cornwall Pumped Storage Plant, pp. 167-204 In: S. Saila, ed.. Fisheries and Energy Production. D.C. Heath, Lexington, Mass. . Becker, A.J., 1977. The respiratory responses of the green crab Carcinus maenas to reduced partial pressures of oxygen. _ EimnoT. Oceanogr. in press. Cinn, T.C. and J.M. O'Connor, 1978 (in press). The responses of Gammarus tigrinus to chlorinated power plant effluents. Estuarin. e Coastal Mar. Sci. I , Ginn, T.C., W. Waller, and G.J. Lauei., 1974. The effects of power plant condenser cooling water entrainment on the emphipod Gamnarus spp. Water Res. 8:937-945. Cinn, T.C. W. Waller, and G.J. Lauer., 1976. Survival and repro duction of Gammarus spp. (Amphipoda) following short-term exposure to elevated temperatures. Ches. Sci. 17:8-16 HONS 012694 O'Connor, J.M. and S. Schaffer. 1977. Survival of striped bass iclithyoplankton in nets. Ches. Sci., 18:312-315. Poje, G.V., T.C. Ginn and J. M. O'Connor. 1978. Responses of ichthyoplankton to stresses simulating passage through a power plant condenser tube. In: Thorp, J. and J.W. Gibbons (eds.). Energy and environmental stress in aquatic systems. Symposium of the Savannah River Ecology Laboratory, 1977. Rachlln, J., A.P. Beck and J.M. O'Connor. 1978. Karyotypic analysis of Hudson River striped bass (fjorone jaxatilis). Copeia, 1978: in press. .......... "................. lubarlk, L.S. and J.M. O'Connor. 1978. A radioisotopic study of mercury uptake by Hudson River biota. I: Thorp, J/ and J.W. Gibbons (eds.). Energy and environmental stress in aquatic systems. Symposium of the Savannah River Ecology Laboratory, 1977. 3. PUBLICATIONS IN REVIEW Ginn, T.C. and J.M. O'Connor. The distribution of Gammarus daiherl in the Hudson River estuary with a note on a range"extension Yor the species. Crustaceans... O'Connor, J.M., K.L. Eichorn and T.C. Ginn, behavioral response of larval and juvenile striped bass to heated and chlorinated power plant effluents. Estuaries... O'Connor, J.M., G. Poje and S. Rigdon. Respiration of larval forms of the striped bass (Morone saxatilis) following short-teria ex- ' posure to elevated temperature's. 'Exp. Mar. Biol. Ecol___ HONS 012695 4. PRESENTATIONS Ginn, T.C., K.L. Eichorn and J.M. O'Connor. 1976. Response of - the striped bass Morone saxati1 is and the amphlpod Gammarus to chlorinated power-plant effluent. Northeast FisR and Wild life Conference. April 26-29, Hersey, Pennsylvania. O'Connor, J.M., C.C. Coutant and J.P. Lawler. 1977. Environmental Impact assessment as ecological, research. Symposium on Energy and Environmental Stress in Aquatic Systems. Augusta, Georgia, November, 1977. . Poje, G.V., T.C. Ginn and J.M. O'Connor. 1977. Use of a condenser tube simulator for evaluating power plant entrainment effects. Northeast Pish and Wildlife Conference, Boston, Massachusetts. April 3-6, 1977. . . ........... ... _ 1977. An operational system for'Simula ting the^pSysical' and chemical environment'of the con denser tubes of a power plant. Symposium on Energy and Wnviron- mental Stress in Aquatic Systems, Augusta, Georgia, November, 1977. ;. ' Poje, G.V. and J.M. O'Connor. 1976. Gammarus tigririus: an estuarine predatory carnivore. Presented to the"Atlantic'Estuarine Research Society, May, 1976. lubarlk, L.S. and J.M. O'Connor. 1977. A radioisotopic study of . mercury uptake by Hudson River biota. Symposium on Energy and Environmental Stress in Aquatic Systems, Augusta, Georgia, November 1977. HONS 0U696 CURRICULUM VITAE . January 1978 Chun Chi tee (Formerly Known as King Mon Tsue) Date and Place of Birth: Marital Status: Residence: Professional Address: Title: Education: Positions Heidi Course Offering: (Department of Environmental Medicine and Department of Biology) ' August 31, 1939 - Kong Kong Married, Two children R.D. 12, Felter Bill Rd. Monroe, New York 10950 New York University Medical Center Institute of Environmental Medicine 550 First Avenue New York, New York 10016 Telephone: (212) 679-3200, Ext. 5276 Research Scientist and Adjunct Assistant Professor Laboratory for Environmental Studies 1962 - B.S. In Zoology Colorado State University 1969 - Doctor of Philosophy in Marine Science University of Miami (Florida) New Yor.k University Medical Center Department of Environmental Medicine 1975 - Present Aquatic and Estuarine Microbiology University of Miami (Florida) Rosenstiel School of Marine and Atmospheric Science, 1969-1975 Research Scientist, Functional Biology 1969 - 1973 Research Assistant Professor Biology and Living Resources 1973 - 1975 MONS 012691 Positions Hold: Professional Societies: Research Interests: Awards and Grants: University of Miami (Florida) Institute of Marine science 1962 - 1969 - NDEA. Fellow, Marine Biology 1962 - 1965 Graduate Assistant 1965 - 1967 Senior Field Biologist McMurdo Sound, Antarctica 1967 - 1968 Senior Field Biologist First International Weddell Sea Oceanographic Expedition . 1968 - 1969 Colorado State University Department of Botany and Plant Pathology 1958 - 1962 Research Assistant, 1958 - 1962 American Association for the Advancement of Science American Society of Limnology and Oceanography Microbiology Algal and Protozoan Physiology and Ecology Biochemistry of Organic Matter in Aquatic sediments ' Achievement Award in Biological Science Colorado State University, 1962 National Defense and Educational Act Predoctoral Fellowship, 1962 - 1965 National Science Foundation Grant University of Miami, $44,000 1973 - 1975 National Science Foundation Grant New York University Medical Center, $3,900 1976 HOMS 012698 publications C. C. LEE 1. Meyers, 6. P., W. A. Feder.and Kincf.Tsue. Nutritional relation ships among certain filamentous f`ungi"and a marine nematode. Science, 141, 520-522 (1963). 2. Meyers, S. P., W. A. Feder and Ki_ng_ Tsue. Studies of relation ships among nematodes and filamentous' fungi in the marine ' environment. Dev. Indus. Microbiol., 5, 354-364 (1964). 3. Altman, J. and KingTsue. Changes in plant growth with chemicals ' used as soil fumigants. Plant Disease Reporter, 49, 600-602 (1965). .4 Bunt, J. S. and C. C. Lee. Observations within and beneath antarctic sea ice in McMurdo Sound and the Weddell Sda. Methods . . and Data. 1MAS Special Publ.,-tlniv. of Miami, 69-1 (1969). 5. Bunt, J. S. and C. _c_.__Lee. Seasonal primary production within - and beneath antarctic sea ice. J. Mar. Res., 28(3), 304-320 (1970). 6. Bunt, J. S., K. E. Cooksey, M. Heeb, C. C.Lee and B. F. Taylor. Algal nitrogen fixation in the marine subtropics: Assay by acetylene reduction. Nature, 227, 1163 (1970). 7. Corcoran, E. F. and C._ C. Lee. The distribution of chlorinated hydrocarbon pesticides" in the' Mississippi River Delta and the adjacent Gulf of Mexico. Joint Southeast and Southwest ACS . Meeting, New Orleans (Abst.). Dec. 2-4, 1970. p. 88. ` 8. Taylor, D. L. and C.,C. Lee. A new Cryptomonad from Antarctica: Cryptomonas cryophlia sp. Nov. Archiv fur Mikrobiol., 75, 269-280 Tl97T)".'....... .~ 9. Bunt, J. S., C.C.Lee and E. L. Lee. Primary productivity and related data Irom "tropical and subtropical marine sediments. Mar. Biol., 16, 28-36 (1972). 10. Bunt, J. S. and C. C. Lee. Data on the composition and dark ' survival of four marinelnicroalgae. Limnology and Oceanogr., . 17, 458-461 (1972). 11. Fcnchel, T. and C. C.Lee. Studies on ciliates associated with sea ice from Antarctica". J. The nature of the fauna. Archiv * fur Protistenk., 114, 231-236 (1972). - 12. C. C.Lee and T. Fenchel. Studies on ciliates associated with sea ice "from Antarctica. II. Temperature responses and tolerances In comparison with ciliates from temperate and tropical habitats. Archiv fur Protistenk., 114., 237-244 (1972). HONS 012699 PUBLICATIONS CONT'D. 13. Bunt, J. S., C;_C._Lee and M. A. Heeb. The site off Freeport intended for Hydro-LaB. Hydro Lab J., Is3-6 (1972). 14. ' Bunt, J. S., C. C. Lee. An exploratory study of benthic primary production. Hydro La~b J., 1^7-10 (1972). 15. Taylor, B. F., C. C. Lee and J. S. Bunt. Nitrogen-fixation associated with the'blue-green alga, Trichodesmlum, as measured by the acetylene-reduction technique. Archiv fur Mikrobiologie, 88:205-212 (1973). 16. C.jC.Lee, E. L. Lee and J. S. Bunt. Distribution of biomass Tn a coral reef transect. In: Results of the Tektite Program, coral reef invertebrates and plants (Sylvia Earle and Robert Lavenberg, editors), Natural History Museum, Los Angeles County, Science Bull., 20:69-75 (1975). HONS 012700 CURRICULUM VITAR July 1977 Theodore J. Kneip Date and Place of Birth December 20, 1926 - St, Paul, Minnesota Marital Status: Married, Six children Residence: 162 E. Fairmount Avenue Maywood, New Jersey 07607 . Professional Address: Hew Fork University Medical Center Institute of -Environmental-Medicine 550 First Avenue New York, New York 10016 .. Telephone: (212) 679-3200, Ext. 5232 Title: . Research Professor, Deputy Director ' Laboratory for Environmental Studies Education: 1950 - B. of Chemistry (with distinction) University of Minnesota 1952 - Master of Science University of Illinois 1954 - Doctor of Philosophy University of Illinois Positions Held: New York University Medical Center Department of Environmental Medicine 1967 -- present Senior Research Scientist 1967 - 1975 Adjunct Associate Professor 1969-1971 Adjunct Professor 1971-1975 ' Research Professor 1975 - . Acting Director, Laboratory for Environmental Studies 1968-1970 Deputy Director, Laboratory for Environmental Studies 1970-present 0Ul RON* Curriculum Vitae Page 2 Course Offerings: (Department of Environmental Medicine) . Analytical Chemistry of Environmental Contaminants. ' Radiochemistry. Positions Held: (continued) Mallinckrodt Chemical Horks 6t. Louis, Missouri 1954-1966 Research Chemist, Uranium Division 1954-1957 Croup leader. Uranium Division 1957-1959 : Head, Analytical Research laboratory 1959-1961 Asst. Manager, Analytical Laboratory, 1961-1963 Uranium Division Manager, Laboratory Products Research 1963-1966 Honor Societies: Phi Lambda Upsilon, 1950 Sigma Xi, 1954 - ProfessionalSocieties: ' . ' ' . American Chemical Society ' American'Association for the ' Advancement .of Science American Public Health Association American Industrial Hygiene Association Air Pollution Control Association American Conference of Governmental ' Industrial Hygienists .. The Hew York Academy of Sciences Vice Ch.air - Environmental Sciences Section - 1976 . . Chair - Environmental Sciences ' Section - 1977 - 78 .. " Elected Fellow - 1977 1 HONS 012702 ^Curriculum Vitae Page 3 Committees: . Consulting: ' . . Chairman of Subcommittee I 6 Metals 1, Intersociety Committee on Manual of Methods for Ambient Air Sampling and Analysis 1971-1977 Member 1971-present Member of International Meeting on Effects and Dose-Response Relationships of Toxic Metals, Tokyo, November 18-23, 1974. Organized by: The Subcommittee on Toxicology of Metals of the Permanent Commission and International Association on Occupational Health. _ Member of American Society for Testing and Materials Committee D-22: Methods of Sampling and Analysis of Atmospheres, Subcommittees D-22.04, 1973-Present. D-22.09, 1975-Present. New.York State - Governor16 ad hoc committee on PCB's, 1975. Secretariat, International Standards Organization/Technical Committee 146 (Air Quality)/Working Group 3 - nitrogen Compounds, 1975-1976. Secretariat, International Standards Organization/Technical Committee 146 (Air Quality)/Subcommittee 2 Workplace Atmospheres, 1976-present. Various corporate organizations including Consolidated Edison, RSR Corporation, Equitable Environmental Health. United States Department of Commerce, National Oceanic and Atmospheric Administration, , Marine Ecosystems Analysis Project, ` ' New York Bight Contaminants Panel. Cornell University, New York State . College of Agriculture and Life Sciences and New York State College of Human Ecology, Cooperative Extension, New York City Urban Gardening Program. HONS 012703 Curriculum Vitae Page 4 List of Published Works: 1. Laltinen, H. A. and_T.. J_s._Sne.ipj_ "Polarographlc and Coulometric Study of p-Dimethylaninoben2ene," Journal of the American Chemical Society, 7_8:730-740 (1956). ~ ......... .......... ....... ' ------------ 3. Xing, P., E. Bretz and T. J. Knelp. "Determination of Iodine in . Mineral Premixes, Analytical Chemistry, 34-.565-567 (1962). 3. jCncip,...T._*r., -T. H.Beasly, P. King and W. K. Dean. "Programmed Temperature Gas Chromatography with Dual Electron Capture Detector for Pesticide Residue Analysis," Analytical Chemistrv, 39:1510 . 1512 (1967). ............ . ' -4i -Cohen, N. and Bne.ip, "A Method for the Analysis of 27Pb ' in Urine of Uranium Miners," Journal_Health Physics, 17:125-130 (1969). . . j. Strehlow, C. D. and J-t- Kneio. "The Distribution of Lead * and line in the Human Skeleton," Journal American Industrial MySijBne_Asspciay_on, 30:372-378. (1969). "' 6. 'Rnein, T. J., G.`P. Howells and M. E. Wrenn. "Trace Metals, *' Radionuclides and Pesticides in the Hudson River," Symposium on Hudson River Ecology, October 1969, Proceedings of-the Symposium, Hew York State Department of Environmental CbnYeVvatYon,'Albany',' New York. . . 7. Purtado, V, C., J?. _JKneip, and M. Eisenbud. "Measurement of Low -Levels of "lj in Reactor Atmospheres," Nuclear Aoplications and Technology, j): 268-273 (1970). .................. .......... ' . .# ***,"" ' , 8 Knelo, T. J., M. Eisenbud, C. D. Strehlow and P. C. Freudenthal. . `"Airborne Particulates in Ne r York City," Journal Air Pollution ?iitrq^l_.As.sj3ci^ation, 20:144-149 (1970). ............. R. Howells, G. P., Koeip. and M. Eisenbud. "Water Quality in ' Industrial Areas: Profile of a River," Environmental Science and Technology, 4:26-35 (1970). ....... ... ....... ' 10. -Xneip, T^_J. "Atomic Abosrptlon Spectrophotometry," AIHA Confer- ence on Advances in Instrumental Method of Analyses, Environmental irece Substances Center,University of"MissouriColumbia, Missouri (May, 1970). - HONS 012704 Curriculum Vitae Page 5 11. ` Laurer, G. R. , .!. .1. -Kneip.. R. E. Albert and F. S. Kent, "in Situ Determination of Lead on Painted Surfaces for the Prevention of Childhood Lead Poisoning," Proceedings of the Third Symposium on Low Enerbv X- and Gamma Rav _Sbuyces''^Rd''A'o?Ticatiqns'f"'Bost'on, Gordon ai^" Breach Science" Publishers',' New'Vbrk'," pp7'289-301' <1971)7 12. . Lentsch, J. W., M. E. Wrenn. T. J_. Xnelp and M. Eisenbud. "Manmade Radionuclides in the Hudson'River Estuary," Proc. 5th Annual Midyear .Topical Symposium, Health Physics Society, Idaho' Falls', Idaho, p."499 (1970). 13. Howells, G. P., M. Eisenbud and <?_ XneiB- "Ecology of the Estuary of the Lower Hudson River,-" Proc. FAO Tech. Conf. on Marine Pollution and_its Effects on^ivin^>e'sources 'and''Fishing, J>uLi7 FIR':M?/70/l> 20, Food" and Agriculture "6rgahiVatio'n of" the United Nations, New York (1970). 14. JCneip.,_T_._0L, "Concentration Factors, Environmental Complexity and the Analytical Chemist," American Chemical Society,'Analytical Division--National Bureau of Standards Summer Symposium: Key to . Progress in National Problem Areas, Washington, D. C. June 1971, Proceedings of the Symposium . 15. Kneip,. 3L.. J..., G. P. Howells and M. E. Wrenn. "Trace Elements, Radionuclides and Pesticide Residues in the Hudson River," Proc.FAOTech. Conf. on Marine Pollution and_ its Effects on Llviifg'Resources' and^Fishing',' PuBl". FIR: MP/7'0/E-20, Fobd'an'd Agriculture'Organization of" the United Nations, New York (1970). 16. Laurer, G. R. _T._ J. Kneijg, R. E. Albert and F. S. Kent. "X-ray *. Plourescence: "Detection of Lead in Wall Paint," Science, 172: 466-468 (1971). "' 17. Xncip.T.J., N. Cohen, D. H. Goldstein and E. A. S. Muchmore, ""Experimental Studies of Lead Toxicity in Trace Substances in Environmental Health - V," Proceedi ngs of Universitv_of Missouri's 5th Annual Conference'on .Trace' Substances'In Environmental Health <ed. by D. D. Hemphill), pp. 349-361, ' ' Tpuhe I97i)7 ' 18. ' Eisenbud, M. and ^._J. ,Kneip_.__ "Trace Metals in the Atmosphere," Tech. Paper .116, New York State Department of Environmental Con servation, Environmental Quality Research and Development Unit (July, 1971). 19. Lcntsch, J. W.,_T. J.. Kneip, M. E. Wrenn, G. P. Howells and M. . Eisenbud. "Stable MangVhese and Menganese-54 Distributions in . * The Physical and Biological Components of the Hudson River Estuary," proc. Third National Symposium on Radioeeology, Oak Ridge (1971). HONS 012 705 Curriculum Vitae Page e 20. Kneip, T._J. "Sampling and Analytical Problems in , H'CbmplVx"Natural System: The Hudson River Estuary," . American Laboratory 3:47-52 (1971). 21. Cohen, N., T.J. Kneip, D. Goldstein and E.A.S. Muchmore, "The JuveniTe Baboon- as a Model for Studies of Lead Poisoning in Children," Journal of Medical Primatology 1: 142-155 (1972). ...................................................... ....... " 22. Kneip, T. J. and G. R. Laurer. "Isotope Excited X-ray ' fluorescence," Analytical Chemistry, :57A-66A (1972). 23. Laurer, G., M. Eisenbud, R. E. Albert, T. J. Kneip and N. Nelson. "New York City Health Research Council X-ray Fluorescence Analyzer for Lead," Isotopes and ` Radiation Technology 9:275-277 (1972). ~ ' *24. JCneipj. T. J., et al., "Tentative Method of Analysis for . Inorganic Lead Content of the Atmosphere," Health laboratory Science 9(l):79-83 (1972). 25. Laurer, G. R., R. E. Albert, T. J. Kneip, B. Pasternack, C. Strehlow, N. Nelson and F. 6-.'Kent,'"'The Distribution of Lead Paint in New York City Tenement Buildings,' AJPH 63: 163-168 (1973)... ------- 26. Xneip, T._ J. and G. J. Lauer. "Trace Metal Concentration factors in' Aquatic Ecosystems," in Vol. 5, Progress in Analytical Chemistry, Plenum Press, New York,'New York'(1973). 27. Ahuja, S., E. M. Cohen, T. J. Kneip, J. L. Lambert, G. Ziwcig, eds. Chemical_Analysis of the Environment and Other Modern Techniques/ 'plenum 'Press,~New' York",~ New" York (1973) / 28. Kleinman, M. T., T. J._Kneig and M. Eisenbud, "Meteorological Influences on AirEorhc Trace Metals and Suspended Particulates," Proceedings, 7th Annual Conference on Trace Substances in Environmental' Health, University of Missouri;''Columbia,' MissourI'," June''12-14 (1973) . .- ^29. JKoeip. , et al., "Tentative Method of Analysis for *Cadmium Content of Atmospheric Particulate Matter," Health Laboratory Science 10(3):214-217 (1973). 90. Kneip, T.J., et al., "Tentative Method of Analysis for Molybdenum "Content of Atmospheric Particulate Matter by . Atomic Absorption Spectrophotometry," Health Laboratory Science 10(3) :218-22Q (1973). . ~ .........- - Reprinted in Methods Of_Air Sampling and Analysis, Second edition, Kats, M., ed. Americah~Public'jfea 1 th Association, Washington, D.C. (1977). HONS 012706 Curriculum Vitae Page 7 *31. Kneip, T. J., et al.f "Tentative Method of Analysis for Silckei'Content of Atmospheric Particulate Matter by Atomic Absorption Spectroscopy," Health Laboratory Science 10 (3) :221-225 (1973). '" '--------- * 32. Kneip, T.J., ct al., "Tentative Method of Analysis for Copper" Content of Atmospheric Particulate Matter by Atomic Absorption Spectroscopy," Health Laboratory Science 10(3)226-230 (1973). ................. ......... ... "............ *33. Kneip,'_T. J., et al.,'"Tentative Method of Analysis for Chromium Content of Atmospheric Particulate Matter by Atomic Absorption Spectroscopy," Health Laboratory Science 10(4)s358-361 (1973). ............ . ............. ........ 34. Kneip, T.' J., G. P. Howells, M. E. Wrenn, "Trace Elements, Radionuclides and Pesticide Residues in the Hudson River," Ini MarincPollutionand Sea Life. Fishing News (Books) Ltd., Surrey", England',' p. X6'9~ (1973). (Reprinted from Ref. 15) 35. Kneip, T. Jw M. T. Klelnman and M. Eisenbud, "Relative Contributions of Emission Sources to the Total Airborne Particulates in New York City," Proceedings, Third International Clean Air Congress^'October 8-T2, 1973 (1974). 36. ' . Cohen, N.,T. J. Kneip, V. Rulon and D. Goldstein, "Biological and Toxicological Response of Infant Baboons to Lead Driers in Paint," Bnv. Hlth. Perspectives, Experimental Issue 17, DHEW Pub. jr~NTH'74-?i8',' 0.s;'Gov't Printing Office, Washington, D.C., p* 161 (1974). 37. Kneip, T. J., N. Cohen and V. Rulon, "potential Sources . of'Analytical Error in the Erythrocytic 6-Aminolevulinic Acid Dehydratase Test for Lead Exposure," Analytical Chemistry, 46:1863-1866, (1974). ------- -------- 33. Bernstein, D. M., T. J. Kneip, M. T. Klelnman, R. Riddick and M. Eisenbud, "Uptake and Distribution of Airborne Trace Metals in Man," Proceedings^of the 8th Annual Conference of TraceSuBstances in Environmental Health, Chlversity" "of Missouri', Columbia,' Missouri,' June' 11974). 39*. *neip, 6. R6 and T. Hernandez, "Cadmium in'en Aquatic Ecosystems Distribution and Effects,* Trace Substances in Environmental Health - VIII, 19747" K~ 8ymppsi"um7" D." D7 Hemphill" 7"ed~.7 ' University of Missouri, Columbia (1974) . HONS 012707 Curriculum Vitae Page 6 40. Bondietti, E. A., F. H. Sweeton, T. Tamura, R. M. Perhac, L. D. Nulett, and T. J. Kneip, 'Characterization of Cadmium and Hickel Contaminated Sediments from Foundry Cove, Hew York,* In: Proceedings of the First Annual NSF-RANN Trace Contaminants ""Conference," Oak' Ridge / Tennessee, August, 1973*. pp."" 2il"-22"4"l CONF-7'30802', ORNL,' Oak Ridge, Tennessee "(1974). *41. Xneip, T.J., et al., 'Tentative Method of Analysis for Cadmium "Content of Atmospheric Particulate Matter by Atomic Absorption Spectroscopy," Health Laboratory Science 11(2):112-116 (1974). ~~......... ................" " *42. Kneip, T. J., et al., 'Tentative Method of Analysis for Iron Content of Atmospheric Particulate Matter by Atomic Absorption Spectroscopy,* Health Laboratory Science 11(2): 118-121 (1974). -- *43. ' Kneip, T.' J., et al., "Tentative Method of Analysis for Eead "Content of Atmospheric particulate Matter by Atomic Absorption Spectroscopy," Health Laboratory Science 11(2): 122-127 (1974). ...... * .44 ' Kneip, T. J., et al., "Tentative Method 6f Analysis for Hanganese'Content of Atmospheric Particulate Matter by Atomic Absorption Spectroscopy," Health Laboratory Science 1 11(2) :128-133 (1974). ........... *45. Xneipy T.' J., et al., "Tentative Method of Analysis for Zinc Content of'Atmospheric Particulate Matter by Atomic Absorption Spectroscopy," Health Laboratory Science 11(2): .134-139 (1974). `` ............. *.......... * .46 Kneip, T. J., et al., "Tentative Method of Analysis for Vanadium Content of Atmospheric Particulate Matter by Atomic Absorption Spectroscopy," Health Laboratory Science ll(3):240-245 (1974). .............. ..... ......... *47. ' Kneip, T.' J., et al. Tentative Method of Analysis for Elemental Mercury in Ambient Air by Collection on Silver Wool and Atomic Absorption Spectroscopy,' Health Laboratory * Science 11(4) :342-346 (1974). HONS 012708 `Curriculum Vit&e Page 9 "*- Xneip, T. J., M. Eisenbud, M. T. Kleinroan and D. M. Bernstein, 'Studies of Trace Substances in an Urban Atmosphere," Proceedings of_ the International Symposium of Recent Advances' in[ the" Assessinent'of` the* HeaYth'Effect's'of" Kivifonniental^ PollutlonTParis7"June' 24-28,"I974""(r975). *49. Goldstein, D. H., T. J. Xneip, V. Rulon and N. Cohen, "Erythrocytic 6-AminolevuIihic Acid Dehydratase (6-ALAD) Activity as a Biological Parameter for Determining Exposures to Lead," Journal of Occupational Medicine, 17:157-162 (1975). 50# Xneip, T-.'j., "Potential Applications of X-ray Analytical * Ketho'd in Paint'Analysis," Journal of Paint Technology, 47:71-76(1975). 51* Xneip, ,T. J., T. Hernandez and Gary Rfe, "Cadmium in an Aquatic Ecosystem: Transport and Distribution," In: Trace Contaminants in the Environment," Proceedings of the Second Annual NSF-RANN Trace Contaminants'Conference, XsTlomarV Pacliic' Groye^' CalifbrjiiaV 29-31' August', 19`7"4' (1975) 52. xneip, T._ J., et al., "Tentative Method of Analysis for Elemental" Mercury in the Working Environment by Collection on Silver Wool and Atomic Absorption Spectroscopy," Health Laboratory Science 12(2):158-162 (1975). 53. Xneip, T. J., et al., "Lead in Blood and Urine," Health Eaboratbry' Science 12 (4) :375-378 (1975). 54. Xneip, T. J., et al., "Mercury in Urine," Health Eaboratory' Science 12 (4) :379-382 (1975). - 55; Xneip, T. J., et al., "General Atomic Absorption Procedure Jor'Trace" Metals in Airborne Material Collected on Membrane Filters," Health Laboratory Science 12(4):383-393 (1975). 56. Xneip, T.J., W. Cohen, V. Rulon, David Goids'tein end'N.~Nelson, "Factors Influencing Dose-Response Relationships for Toxic Metals," In: Effects and Dose-Response Relationships of Tox_ic_Metals', G.TT Nordberg, id. ; Associated ScientificPublishers,'Amsterdam (1976). 57 .Eisenbud,-M., T. J. Xneip, M. T. Xleinman, D. M. Bernstein, "Trace Metals in Urban~Aerosols," Final Report to Electric Power Research Institute, October, 1975. JWIS I Pb-248-324 (1976). . HONS 012709 Curriculum Vitae Page 10 58. Bernstein, D. M., M. T. Kleinman, T. J. Xneip, T. 1. Chan and M. Lippmann, "A High-Volume Sampler Tor'the Determination of Particle Site Distributions in Ambient Air," Journal Of the American Air Pollution Control Association '2'6 (1)), leer-low imsj:------------------- -------------------------------- ----------- ----- 59. Kleinman, M. T., T. 0. Xneip and M. Eisenbud, "Seasonal Patterns of Airborne Particulate Concentrations in New York City," Atmospheric Environment 10:9-11 (1976). 50. Xleinman, M. T., D. M. Bernstein and T. J. Xneip, "An Apparent Effect of the Oil Embargo on Total'Suspended Particulate Matter and Vanadium of New York City Air," J. Air Poll. Con. Assoc. 27:65-67 (1977). ` '61. Xneip,T.J., R. Peter Mallon and Virginia P. Rulon, 'The Effects of Anemia on Heme Synthesis Parameters During Dead Exposure," American Industrial Hygiene Association Journal 17,- 578" -58(1977) ; ' " " 62. Xneip, T. J., et al., "Analytical Method for Antimony In Air"aha''Urine," In;' Methods of Air Sampling and Analysis, Second edition, Katz, M. / ed".'American Public Health Association, Washington, D. C., pp. 765-769 (1977). 63. Xneip, T. J., et al., "Analytical Method for Arsenic In'Urine `and Air," In: Methods Of_Air Sampling and Analysis, Second edition, Katz, M7~, ed7`American "Public Health'....... Association, Washington, D. C., pp. 769-773 (1977). 64. Kneip,_ T/ J., et al. , "Analytical Method for Arsenic, Helen!uni and Antimony in Urine and Air by Hydride Generation and Atomic Absorption Spectroscopy," In: ' Methods of Air Samplijig jind^ Analysis, Second edition, . Ratz, M., ed.AmericaTi Pub)ic'Health Association, Washington, D. C., pp. 774-780 (1977). ` 65. ' Xneip, T. J., et al., "Analytical Method for Chromic Acid Hist in'Air," In: Methods of AirSampllng and Analysis, Second edition, Xatz, M.',"ed".'American PuBirc'HealtK" Association, Washington, D. C., pp. 785-788 (1977). 66. Xneip, T. J., et al., "Analytical Method for Dead, in Air," XnT'McthocTs of Air Sampling and Analysis, 6econd edition, Xatz7 M., ed7American public Health Association, Washington, D. C., pp. 808-818 (1977). 67. Hazen, R. E. and T. J. Xneip, "The Distribution of Cadmium in the Sediments of~'Fouhdry Cove," In: Hudson River Ecology, Proceeding of a Symposium, Hudson River EnvironmentalSociety,'etiV 'March 28-30, 1976 (1977). HONS 012710 Curriculum Vitae Page 11 68. Xneip, T. J., M. T. Kleinman, R. Riddick and D. M. Bernstein, "Trace Elements in Human Tissues," Proceedings of the International Workshop on_Biologica3_Specimen Collections, April'18-22'," 1977"."Xuatembdurg'," (In'press', 1977'). ...... 69. Kneip, T. J., "Effects of Cadmium in an Aquatic Environment," In: Cadmluni 77, Edited Proceedings First International Cadmium Conference, San_Francrsco", "31 Jan.-J Feb., 1977. MetaT Bulletin Limited', London, pp. 120-124, (1978). 70. Daisey, 0. M., I. Hawryluk, T. J. Kneip and F. Mukai, "Mutagenic Activity in Organic Fractions of Airborne Particulate Matter," Presented at the Carbonaceous Particles in the Atmosphere Conference, March 20-24, 1978, Berkeley, California. 71. Kneip, T.'J., M. A. toyko, M. T. Kleinman, M. Lippmann and J. M. Daisey, "Organic Matter in New York City TSP: Comparisons Of Seasonal Variations and Relationships to Source Tracers," ' Presented at the Carbonaceous Particles in the Atmosphere Conference, March 20-24, 1978, Berkeley, California. 72. Lioy, P. J,, G. T. Wolff and T. 3. Kneip, "Toxic Airborne Elements in the New York Metropolitan Area," 3. Air Poll, ton. Assn., 28(5) >.510-512 (1978). ~ ~.... 73. tenderer, 8. P., D. M. Bernstein, 3. M. Daisey, M. T, Kleinman, T. J. Kneip, E. O. Knutson, M. Lippmann, P. j. Lioy, K. A. Rahn, D. Sinclair, R. L. Tanner, and G. T. Wolff, "Summary of the New York Summer Aerosol Study (NYSAS)," 0. Air Poll. Con. Assn., 28(4):321-327 (1978). 74. Fennikoh, K. B., H. I. Hirshfield and T. J. Kneip, "Cadmium Toxicity in Planktonic Organisms of a Freshwater Web," Environ. Res. (In press, June, 1978)/ 75. Kneip, T. J. and M. T. Kleinman, "Sampling and Multielement Analysis of AJnbient Aerosols," In: Special Environmental Report No. 10, Air Pollution Measurement Techniques', Proceedings of the" WMO "Air Pollutiori Measurement" Technique's Conference (APOMET), Gothenburg, 11-15 October, 1976. World Meteorological Organization, Geneva, Switzerland (1977). 76. tourer, G. R. and T. J. Kneip, "Portable X-ray Units for Analyzing toad in Paint," In: A Report of the Committee on ' Environmental Improvement:JjKssessing th'e Proble'm_of Lead-Based taint Hazards, pp. 13-17, American Chemical Society, ' ........ - Washington, D.C. (August, 1978). HONS 012711 , CURRICULUM VITAE JOSEPH MARTIN O'CONNOR PERSONAL EDUCATION HONORS AWARDS SOCIETIES AFFLILIATIONS Born: November 19, 1944, Boston, Massachusetts Married: 2 children .. . Bachelor of Science, Biology (Philosophy minor) June, 1966 . College of the Holy Cross Worcester, Massachusetts Doctor of Philosophy, Ecology/Physiology November, 1971 . State University of New York at Albany Albany, New York Dissertation Title: ' Photoperiodic control of pituitary gonadotropin release in trout (Salmo qairdneri Richardson and Salvelinus fontinalis (Mitchell) and in the leopard-'frog '(Rana'plpiens Shreber) Sigma Xi Beta Beta Beta Biological Society Student representative to Faculty Senate State University of New York at Albany R.E.S.A. (Sigma Xi) Research Grant-in-Aid N.S.F. Pre-doctoral fellowship (Honorable) Research Grant-in-Aid,' Edmund Niles Byuck Preserve Fellowship, Research Foundation of New York State New York Academy of Sciences American Society of Limnology and Oceanography Estuarine Research Federation Atlantic Estuarine Research Society AAAS American Society of Natural History Ecological Society of America American Fisheries Society Sigma Xi Great Lakes Research Bociety Florida Defenders of the Environment Board of Consultants, Lawler, Matusky and Skelly Engineers, Tappan, New York HONS 012712 EXPERIENCE present 1974-75 1973-74 1970-73 1967-70 1965-67 (summers) PROFESSIONAL ACTIVITIES Research Assistant Professor Department of Environmental Medicine, and Assistant Director ' Laboratory for Environmental Studies New York University Medical Center Sterling Forest, Tuxedo, New York Senior Biologist Office of Professional and Technological Development Lawler, Matusky and Skelly Engineers 415 Rt. 303, Tappan, New York Director Biological Studies Section and \ Laboratory Office of Research and Technology Quirk, Lawler and Matusky Engineers and QLM Laboratories, Inc. Tappan, New York Research Associate University of Maryland Natural Resources Institute Hallowing Point Field Station Prince Frederick, Maryland Graduate Research Fellow Dept, of Biological Sciences State University of New York at Albany Faculty Research Assistant University of Maryland Natural Resources Institute Chesapeake Biological Laboratory Solomons, Maryland Member, Advisory Committee Chesapeake Bay R.A.N.N. Program of the National Science Foundation 1971-1972 U.S. Dept, of Agriculture Graduate School Programs in Aquatic Ecology and Pollution Biology Counselor Rockland County Guidance Center for Women Guidance in Career Programs Adjunct Assistant Professor Inst, of Mar. and Atmos. Sciences of the City University of New York HONS 012 713 3 Advisor, Fisheries Biology 0.8. Dept, of Commerce National Oceanic and Atmospheric Administration New England Offshore Mining Environmental Study 1972-1974 Visiting Investigator University of Delaware . College of Marine Studies Bayslde Laboratory . Lewes, Delaware 1972 RESEARCH INTERESTS ~ Population dynamics Community dynamics Respiratory physiology of fishes Reproductive physiology of fishes Chemical-biological interrelationships in ecology Ethology of fishes TEACHING INTERESTS ........... ' . Physiological ecology General physiology General ecology Pollution biology , Ichthyology PUBLICATIONS O'Connor, J.M. 1969. The effects of melatonin on in vitro ovulation of frog ovarian oocytes. Amer. Zool. 9:5497....... O'Connor, J.M. and R.G. Stross. 1970. Photoperiodic control Of the pituitary gonadotropin release patter of the frog, Rana pipiens Shreber. Abstr. Proc. N.E. Division Comp. Physiology section, Amer. Soc. Zool. O'Connor, J.M. 1970. The effects of thermal shock on the tidal activity patterns of the hogchoker, Trinectes maculatus. Abstr. Amer. Society of Ichthyologists and Berpetofogi 6ts, Ann. Mtg., New Orleans, La. O'Connor, J.M. 1971. Photoperiodic control of the pituitary gonadotropin release patterns in the rainbow trout (Salmo galrdnerl (Rich.,)), the brook trout (Salvelinus fontinalis JutchelTjf and in the leopard frog (Rana pipiens Shreber). Ph.D. Thesis. Library, State University of New York at Albany, and University Microfilms, Ann Arbgr, Mich. . HONS 012714 4 O'Connor, J.M. 1972. Pituitary gonadotropin release patterns in rainbow trout (Salmo gairdneri) brook trout (Salvelinus fontinalis) and in"the'leopard frog (Rana pipiens')'.'' Co'nip. MocKemV Physiol. 43(A):739-746. ~ O'Connor, J.M. 1972. Tidal rhythm in the activity patterns of the hogchoker, Trinectes maculatus (Bloch and Schneider). Exp. Mar. Biol. EcoiV 9:173-178. Sherk, J.A., J.M. O'Connor and D.A. Neumann. 1972. The effects of suspended and deposited sediments on estuarine organisms. Univ. Md. N.R.I. ref. 72-9. Sherk, J.A., J.M. O'Connor and D.A. Neumann. 1973. The effects of suspended and deposited sediments.On estuarine organisms. Univ. Md. N.R.I. ref. no. 73-4. O'Connor, J.M., R.W. Williams and J. Converse. 1974. Studies of fish impingement at the Nine Mile Point Nuclear Station, Lake Ontario. Abstr. 17th Conference on Great Lakes Re search. Iht'l Assn, for Great Lakes Research. O'Connor, J.M. and J.A. Sherk. 1974. The responses of some estuarine organisms to concentrations of suspended solids. Proc. of the Seventh Dredging Seminar, Ctr. for Dredging Studies, Texas AIM University. Sherk, J.A., J.M. O'Connor and D. Neumann. 1975. The effects of suspended sediments on estuarine environments. IN: L.E. Cronin (Ed.). Estuarine Research, Vol. II. Geology and Engineering, Academic Press, N.Y. pp. 541-557. , Neumann, D.A., J.M. O'Connor and J.A. Sherk. 1975. Respiratory and hematological responses of the oyster -toadfish (Opsanus tau) to suspended sediments. Trans. Amer. Pish. SocT~ ' ' 104:775-781. * Sherk, J.A. and J.M. O'Connor. 1976. Effects of sedimentation on coastal rone organisms. Proc. Ill Federal Inter-Agency Sedimentation Conference. O'Connor, J.M. and S. Schaffer. 1977. Effects of sampling gear on striped bass ichthyoplankton. Chesapeake Science 18:312-315. 6herk, J.A., J.M. O'Connor and D.A. Neumann. 1976. Effects of suspended solids on selected estuarine plankton. U.S. Army Corps of Engineers Coastal Engineering Research' Center. Misc. Rept. 76-1. 50 pp. Jan. 1976. . HONS 012715 5 O'Connor, J.M., D.A. Neumann, and J.A. Sherk, Jr. 1976. Lethal effects of suspended sediments on estuarine fish. Technical Paper No. 76-20, U.S. Army, Coastal Engineering Research Center, 38 pp.' O'Connor, J.M., D.A. Neumann, and J.A. Sherk. 1977. Sublethal effects of suspended sediments on estuarine fish. Technical Paper No. 77-3, U.S. Army, Coastal Engineering Research Center, 90 pp. ' Rachlin, J., A.P. Beck, and J.M. O'Connor. 1978. Karyotypic analysis of Hudson River striped bass, Morone saxatllis (Walbaum). Copeia 1978(2):334-336. ................ ... Cinn, T.C. and J.M. O'Connor. 1978. Responses of the estu- ' erlne amphipod Gammarus daiberi to chlorinated power plant effluent. Estuarine Coastal Mar. Sci. 6:459-469. . Poje, G.V., T.C. Ginn, and J.M. O'Connor. 1978. An opera tional system for simulating the physical and chemical en vironment of the condenser tubes of a power plant.' IN: Thorp, J. and J.W. Gibbons, eds. Energy and Environmental Stress in Aquatic Systems (in press). tubarik, L.S., and J.M. O'Connor. 1978. A radioisotopic study of mercury uptake by Hudson River biota. IN: Thorp, J. and J.W. Gibbons, eds. Energy and Environmental Stress in Aquatic Systems (in press). . O'Connor, J.M., C.C. Lee, and M. Eisenbud. Environmental Health. M.E.S.A. New York Bight Atlas Monograph 32 (in press). .' Ginn, T.C., G.V. Poje, and J.M. O'Connor.. 1978. Survival of planktonic organisms following.passage through a simu lated power plant condenser tube. Proc. Fourth Nat'l Workshop on Entrainment and Impingement, pp. 91-101. PUBLICATIONS IN REVIEW O'Connor, J.M., D.A. Neumann and J.A. Sherk. The responses of estuarine fishes to highly turbid environments. Water Res. O'Connor, J.M. and J.A. Sherk.. The effects of suspended mineral solids on the white perch, Morone emericana. Trans. Amer. Fish. Soc. ~ ~ O'Connor, J.M. and J.A. Mihursky. The effects of thermal shock on the activity patterns of: the hogchoker, Trinectes maculates. Ches. Sci. " * MONS 012716 6 MANUSCRI FTS_IN_ PREPARATION Eichorn, K.L., T.C. Ginn and J.M. O'Connor. Response of striped bass (Morone saxatilis) to chlorinated cooling water. O'Connor, J.M., G.V. Poje, and 6. Rigdon. Respiration of striped bass (Morone saxatilis.) eggs and larvae. Becker, A.O. and J.M. O'Connor. Aerobic shutdown in the green crab (Carcinus maenas) in response to temperature, salinity and'suTfide. - - Steinhausler, F., S. Schaffer, N. Cohen, C. Lee, J. O'Connor and M. Hrenn. Effect of high LBT radiation on intracellular ATP content of prokaryotic and eukaryotic algae. Hendrick, E., J.M. O'Connor, end G.R. Laurer. A method for analysis of trace mineral content of fish otoliths using X-ray fluorescence spectroscopy. - P RE SENTATIONS O'Connor, J.M. 1969. The effects of melatonin on in vitro ovulation of ovarian oocytes in the frog, Rana pipTens.` Amer. Soc. Zool. O'Connor,. J.M. and R.G. Stross. 1969. Photoperiodic control of the pattern of pituitary gonadotropin release in the frog Rana pipiens Shreber. Amer. Soc. Zool. O'Connor, J.M. 1966. Alterations in the tidal activity rhythm in the hogchoker after exposure to thermal shock. Atlantic Estuarine Research Society, Cambridge, Md. October, 1966. O'Connor, J.M. and J.A. Sherk. 1971. The lethal effects of suspended solids on estuarine fishes. Joint Mtg. Atlantic Estuarine Research Society and the Estuarine Research Federation. Pleasantville, K.Y. October, 1971. Neumann, D.A., J.M. O'Connor and J. Sherk. 1972. Studies of the respiration of the striped bass and the white perch . exposed to suspended sediments. Atlantic Estuarine Research Soc. Ocean City, Md. April, 1972. O'Connor, J.M., J.A. Sherk and D. Neumann. 1973. inert" mineral compounds as potential factors in mortality and toxin transport in estuarine organisms. E.P.A. Workshop on Chemical Pathogens in the Environment. Pensacola, Fla., May, 1973. HONS 012717 * 7 Sherk, J.A., J.M. O'Connor and D. Neumann. 1973. The effects of suspended solids on estuarine environments. Symp. on Recent Advances in Estuarine Research. II Int'l. Conference of the Estuarine Research Federation. Myrtle Beach, S.C. October, 1973. O'Connor, J.M., and J.A. Sherk. 1974. The responses of some estuarine organisms to concentrations of suspended solids. Seventh Dredging Seminar. Sp. by the Center for Dredging Studies, Texas ASM University, New Orleans, La. November, 1974. O'Connor, J.M., R. Williams and J. Converse. 1974. Impinge ment studies at the nine mile point nuclear power station, ..lake Ontario. 17th Conference on Great Lakes Research, August, 1974. Hamilton, Ontario,.Canada. O'Connor, J.M., and S.G. O'Connor. 1975. Seasonal Compartmcntali- sation of primary productivity in the New York Bight, 1973-74. Am. Boc. of Limnology and Oceanography, Halifax, N.S., Canada, June, 1975. ' O'Connor, J.M. 1976; Assessment of entrainment impact at the Indian Point Nuclear Station. Third National Work shop on Entrainment and Impingement, New York City. Poje, G.V. and J.M. O'Connor. 1976. Gammarus tigrinus: an estuarine predatory carnivore. Presented to the Atlantic Estuarine Research Society. May 1976. Ginn, T.C., K.L. Eichorn and J.M. O'Connor. 1976. Response of the striped bass Morone saxatills and the amphipod Gammarus to chlorinated power-plant effluent'.' Northeast Fish and wild life Conference. April 26-29, Hershey, Pennsylvania. Pojc, G.V., T.C. Ginn and J.M. O'Connor. 1977. Use of a condenser tube simulator for evaluating power plant entrain ment effects. Northeast Fish and Wildlife Conference, Boston, Massachusetts. April 3-6, 197?. Poje, G.V., T.C. Ginn, and J.M. O'Connor. 1977. An operational system for simulating the physical and chemical environment of the condenser tubes of a power plant.-' Symposium on Energy and Environmental Stress in Aquatic Systems, Augusta, Georgia, November, 1977. ' Eubarlk, L.S., and J.M. O'Connor. 1977. A radioisotopic 6tudy of mercury uptake by Hudson River biota. Symposium.on Energy and Environmental Stress in Aquatic Systems, Augusta, Georgia, Novcnfoer, 1977. . HONS 012718 s O'Connor, J.M.,C.C. Coutant, and J.p. Lawler. 1977. Environ mental impact assessment as ecological research. Symposium on Energy and Environmental Stress in Aquatic Systems, Augusta, Georgia, November, 1977. O'Connor, J.M., T.C. Ginn, and G.V. poje. 1978. Survival of planktonic organisms following passage through a simulated power plant condenser tube. Fourth Nat'l Workshop on En trainment and Impingement. Chicago, Illinois. Steinhausler, F., S. Schaffer, N. Cohen, C. Lee, J. O'Connor, and M. Wrenn. 1978. Effect of high LET radiation on intra cellular ATP content of prokaryotic and eukaryotic algae. Radiation Research Society. Toronto, Ontario, Canada. HONS 012719