Document nd0V0NLrrkyo3OMr0w5JKgnR

CorporateHealth Physics CorporateOccupadonal Medicine CorporateProduct Responsibility Corporate Toxicology 3M Medical Department 3M Center,220-2E-02 PO Box 33220 St Pa4 MN 55133-3220 651733 1110 AnalysisofSelectedDecatur Employee Serum forSulfonicand Carboxylic ]Fluorochemicals This3M technicarleportanalyzedthe5 highestorganicfluorinceontaininDgecatur employee samplesand analyzedtheserum forperfluorooctanesulfonate, perfluorohexanesulfonaatned perfluorooctanoateI.n decreasingpercentfound, perfluorooctanesulfonactoentributebdetween 55 - 80%, perfluorooctanoatceontributed between 15 - 300/oand perfluorohexanesulfonactoentributed5 - 15% oftheorganic fluorineA.nalyticalmethods used were electroncaptureand microwave plasma detector. 3M T EOCHNICAL REPORT Report Number-. 7230 Date: October 4, 1979 TITLE: Analysis of Selected Decatur Employee serum for Sulfonic and carboxylic pluorocbealeals AE)THORs ABSTRACT: The precence of C and C. sulfonic acids was detected in certain blood samples using bo;g electron capture and microwave plasma detector methods. CENTRAL ANALYTICAL LABORATORY Report No -------7-23-0----------- October 4. 1979 Date ------------ Lalubject: Characterization of Decatur RF Values Pequestor: Dept. Name Request No. ------- Dated _ kcS2ber 12ZI Report: The serum of 10 selected 3M Decatur employees was submitted (A72754) in June, 1979 for analysis of organic fluoride levels and the total organic fluoride values were reported in Analytical -Report7194. Subsequent to that work, It became of interest to further characterize the fluoride serum value and attempt to Identify the presence of 3M fluorochmicals. Initial analysis (7/18/79) by the microwave plasm detector (MPD) established the presence of the expected per fluoronctanesulfonate and perfluorooetanoate anions in the serum extract of - -25 and . -22. However, the observation of perfluorohexanesulfonate anion in the above extracts was unexpected and it therefore became necessary to spend additional analytical development time in order to measure these 3 fluorochemicals in the same serum extract sample. During this time, the method was evaluated on the electron capture (EC) gas chromatograph. The EC detector would be expected to have greater sensitivity than the KPD for these fluorochemicals but with less specificity since the MPD can be operated on the fluorine channel eliminating any confusion by other EC active compounds. We selected the 5 highest fluorine containinr Decatur samples and analyzed the serum extract for perfluorobexanesulfonate, perfluorooctanesulfonate, and perfluorooctanoate anion. We used both the HPD and EC detection systems and report the following values: % IFOUND(D Person -22 -23 -24 -25(D -26 RF 10.1 5.7 9.4 11.8 4.1 Perfluorohexanesulfonate 5% 15% 5% 5% 10% Perfluorooctanesulfonate 60% 70% 80% 55% 65% Perfluorooctanoate 30% 20% 15% 20% 25% Total 95% 105% 100% 80% 100% (Drhe analysis was conducted on a single determination (1 al of serum) due.to limited availability of sample and time and represents our interpretationof the averare of the EC and MPD values. No internal standard was used; expect tlO-20% accuracy. (I)A,6R962, 7028, 7194 epreviously identified as -21(Z) due to mia-labaling by medical personnel. :4o. 7230 .t*lct.obe4r, 1979 L"age 2 The above results are necessarily somewhat approximate due to the lack Of an internal standard and contim"-l refinements in the derivatization and C;Cof the corresponding mechyl aster, Sufficient sample remains for additional analysis. Ytwther improvements in the analysis are and will continue to be made as time permits. We thought it beat to report these values now, especially &I=* we Identified the presence of perfluoroboxanesulfonate In human serum extract and no toxicology studies to date, have been completed an this fluoroche"cal. Throe years ago (10-13-76) we reported that Decatur =*lay&* serum extract contained perfluorooetamoulfonate (based on T/M) and a minor fluorim* containing component now identified as perfluorooctano&te. Therefore, the presence of per- fluorooetanesulfon&te in this Individual Me been known for som time Nowg with improved analytical methods, we *have greater sem:it:iv:LtyaW em determine the presence of perfluorohazanesulfonate In addition to perfluorooetammulfonate anion.