Document nZLGNYwj5XNZ294KOm4mJbba

`Toxicological Summary PFOS Dietary Chronic Definitive Reproductive Study: Northern Bobwhite Test Substance: Perfluorooctanesulf(oPnFaOtSe) Structure: d1e-cOacftlaunoersou,lfpoontiacssaicuidm,sa1l,t1,2C,2A,3S,3N,o4..4,257,955,-63.69,-73,.7.8.8,8-hepta- Test Remarks: TFCh-e95t.est Tsuhbestsaanmcpeleiswaaswhsioterepdouwnddeerr(a3mMbiLeontt #c2o1n7d)i,tiaolnssoaknndopwunriatsy awnaaslysdiestetremcihnnieqduetso. be 869% by LC/MS, 'H-NMR, and elemental METHODS Method: ASTM Standard E1062-86, OECD 206, and FIFRA Subdivision E., Section 714 `Type: Dietary Reproduction ear: 2001in-life phase, 2002-2003 analytical phase, Final report in 2003 Species: Northen bobwhite quail (Colinus virginianus) Experimental Design: Adult northern bobwhite quail were exposed to PFOS at nominal `wceoenkcse.ntrEaatciohnstroefat0m,en1t0,gr5o0u,pocron1t5a0inpepdm16inpatihresodfiqetuaolv,eronaeppeariirodpeorfpaenp.proIxniamdadtietlyio2n41, dotuhreirngpathies toefst.quaTihlewepreensmathianttawienreed upseerdtrfoeratbmleonotdacnodllwecetrieonusdeudrifnogrtbhleotoedstcowlelreectinoont ubsyeWdeteokas3s,estsheretprreoadtumcetnitonl.evDeluewatosorveedrucseidgntsoo2f0tpoxpimc.ityTnhoeted15i0n72t0hep1p5m0 dpopsme wtraesattmheennt stiegrnmsinoaftetdoxiactitWy.eekAll5. emTahieni5n0gpapdumltdoqusaeilwawserteerombisneartveeddatdaiWleyefkor7,siaglnssoodfuteoxtiociotvyerotr abnormal behavior. Body weights were measured at Weeks 2, 4, 6, 8 and at study teexrtmrianaptaiiorn.of Faedueldt cqounasiumapttifoinvew-awesekmeiansteurrvealds.weeAklly.timBeloofodadwualts tceorlmliencatteidonf,robmlotohde sfarmopmletshewe1r5e07c2o0llpepctmedtrferaotmmenatl. suArlvlivbinogbwqhuiatiel fqourailc,heemxicceaplt afnoarlystihso,seexicnluthdein5g0quaanidl 150120 ppm treatments, were cuthanized and subjected to gross necropsy. At the time of necropsy, tissues were. collected for histopathological examination and chemical analyses. OAdnuclet eqguagilprwoedruectbiroonugwhatsinitnoititahtiedr, reegpgrsodwucetrievesepthawseeekblyypfhoortoisntciumbualtaitoino.n aEtgWgesewker7.e 1 CRATE ceagngdiseetd.sRoetphraotdtuhcetidveeveelnodppmoeinnttaslesvtaalgueaatendd adunryinagbnothrimsalsittuideys cionuclluddbede:reecgogrdperdodfuocrteioanc,h echmibcrkysowveiraebilfietdy,ahnatucnhtarbeialtteyd, adinedt hfoartc1h4indgayhse.althAtantdhesuernvdivaolf. thAeftsetrudhya,tcchhiincgk, bquoadiyl sweaimgphltesswweerreedectoelrlmeicnteedd.fPrroimor1o0 ecuhtihcaknsasfiraoomfbtohtehlatshtegrcoonutproolf oafnfdspr1i0ngp(pLmottJr)e,abtlmoeondt groups for chemical analysis. In addition, tissues were collected from these chicks for nhiesctrooppastiheodloqguiacial, aenxdamtihneatliivoenr waansd saanmapllyseids.forLicvheermicwaeligahntaslysiwse.reSarmepcloerdsedwerfeoralaslol toafkFeanbroifcithues lfvoerr,hibsrtaoipna,thkoildongeiyc,algoenxaadm,inpartoivoennirfircoumlusb,otghalladbullatddaenrd, oafdfispporsien,gabnodbwBhuirtsea quails. Test Bird Age: Adult quail were 24weeksold at test initiation. NanudmboneerfoefmaRleeplaidcualttesb:irTdhepreer wpeern.e 1I6npaadidristioofn,ad4ultpaqiurasilofprquatirleawtemreentmwaiitnhtaoinneedmapleer {reatmeanstpartoftheblood monitoring study. sFteueddy taondbotWhataedurl:tsFaonoddoaffnsdprwiantg.erFweeerdecpornosvuimdpetdioandwlaisbimteumasduurreidngonalla ppehraspeesnofbtashies, once a week. Analytical Monitoring: PFOS concentrations in feed, red blood cels, lver issue, sera aEngdgccgogmpcoonmepnotnsewnesrewesreepadreatteedrmiusniendgbay mreetvehrosde-cphhaarsaectHePriLzeCd abnyd BmeamsasrdsptecatnrdomCetoroyk. (1960)and Sifani et al.(1990). Statistical Methods: Upon completion of the study, an analysis of variance (ANOVA) wmualstippelrefcoormmpeadrtiosoenvalpuraotceedsuirgenifwiacsantusdeidffteorecncoemspabreetwPeeOnStretartemaetnmtengtrogurpso.upsDuwninhettth'es controls (Dunnett, 1955; 1964). The sample units were the individual pens within cach tbirreda.tmeStnattigsrtoicuapl,aenxacleypstesfoofrbaodudlyt bwoeidgyhtwseiwgehrtes wcohnedruecttheedssaepmaprlaeteulnyitfowramsaltheeainnddifveimduaalle `bmoebtwhhoidtefoqlulaoiwling Paerrcsciennetasgqeuadraetaroo(ftetprraondsufcotrimoantiodna.ta)StwaetirseticeaxlaamnianlyesdisuosifntgheDudnanteatwt'ass pSoefrtfwoarrmeedpacuksaigneg dAevviealonpeRdebpryoWdiulcdtliiofne IDnattemaatiSoynsatle,mLid(.ARDS) Software, a. validated AbavseirsaagnedDadiildynIonttatkaeke(AinDtIo)aocfcoPuFntOSpotfeonrticaalchditfrfeeartemnecnets gbreotuwpeewnastheestmiamlaeteadnodnfaempaelne wthietheixnpoasuperne. duFraotoidoncoofntshuempsttiuodny aanndd tahdeulAtDqIuawialsbocadlycuwleaitegdhtasdaftoallowwesr:e averaged over 2 [elsirdrard (rghody weigh doy. AC AevBeErFaegxeo dd Consun mpinaeedds ilinty)) ptc ADI mghghody weigh day) AverageBodyweight (g/bird) 9m) "wTaesstuDeiedtiPnraepwaereaktliyonp:reTphaerattieotndoifetthseweirneaprdeicpta.reHdomboygmeinxeiintgy PofFOPSFOiSntoinatphreedmiicxt wthaast evaluated throughout he sty. RESULTS: fMeecadssuarmepdleDsiewterCeonccoelnltercatetdioannsd: aTnoalyvzeerdi.fy tMheeacnosncaenndtrasttainodnaridn tdheeviPatFiOonSsotfrteahtementtess,t doifctcsonatraingiinvgenPF(TOaSb.le A1)n,alFyseedsosfatmphleesdifetrocmontfhiercmoendtrtohlattrPeaFtOmeSntwsshoswteadbenoanidnddiicdatniootn degradeduringthe sudy "Table 1. Meandastandnard deviationsofPFOS concentrations inthediet of nCoorntcheeNnrtnorabmtoibaownhsitoefquailMe4asureodfCPoFnOcSentrations ov Poem PFOS (ppm) pom 0) Nomimi Con2t1r00ol (0) 120<02L82O20Q2'51543 sa10d3 110024 15500 51069122210564 940616 110082 LQcmiosneseitom f quane to (L0e G20 por) Minortthaelictoinetsroalnodr C1l0inpipcamltOrbesaetmrevnattsi.onsH:oNweovterrc,atsmtearntti-nrglsatteWdemerkta5,licliisnicwaelresiognbssewrevreed roebascetrivoendtoaemxotnergnaqlusaiimuiln,trhueff1e0d appppmeartarneactem,enatn.d leStihganrgsy.of toxicity included reduced Wpiptmhitnrethatmefnirts,t a5nwdeetkhseoefmtohrtealsittuideys,ofcicvuer-rteedatinmetnhter1e5la0t7e2d0mporptmalittrieeastmoenctc.urTehde ienxptihrein5g0 QSuuarivliveixnhgibqiuatield oivnetrhtes1ig5n0s/2o0fptpoxmicittryeaptrmieonrtowedreeatch.thaDniuzeedtoaPtFWOeS.erkel5a,taenddefsfuercvtis,vianlgl qiuncaliuidnedthree5du0cpepdmretarcetaitomnensttiwmeurlie,euwtihnagnizderdoospt,Wleosss o7.f cSoiogrndsinoafttoixoin,citlyosast othfesreigdhotsiensg arpefpleeaxr,ancleo,welrowleimlb.imbriwgeiadiktnye,ssc,olnevtuhlasrigoyn,s,gapisnhga,llporwostraantde porsatpuirde arenspisrpaatsimosn., ruffled (BaLsOeAdEoCn) cwlainsicdaeltseirgmnisneodf ttooxbiecit1y0, ptphme.Lowest Observable Adverse Effect Concentration 3 . 0778 eAfdfuelctts BoondyboWdeyigwheti:ghtCoammpoanregdqutaoilconitnrotlhse, t1h0erpepwmerterenatomeanptpa(rTeanbtletr2e)a.tmenHto-wreevleatre,d swteairgthitngs aatmoWnegekqu2a,ilthinertehewa5s0 aanmdar1k5e0d20anpdpmstattrisetaitcmaelnltys.signBiafsiecadntonretdhuecsteiornesuilntsb,otdhye b0odbyew5e0igphptmL,owwehsitleObtsheerNvaoblOebsAedrvvearbsleeEAffdevcetrCsoencEefnfterctatCioonnc(enLtOrAatEiCo)n w(aNsOAdeEtCe)rmiwnaesd determined to be 10ppm. Feed Consumption: There were no treatment-relaed effects on feed consumption for qstuaarltinign attheWe1e0kpp1,ma ttrreeaattmmeenntt.grreoluaptewdhreednucctoimopnairnedfeteod ccoonntsruolmspt(iToanblweas2).obHseorwveevderi,n quai from the 50 and 150/20 ppm treatments. While some recovery in feed consumption dwuaes 1o0bsaerrveedducitnitohnei1n50t/he20PpFpOmStdreoasteme(nftrogmrou1p50at10We2e0kp4p,mthiisn itmhperofeveedm).entDwuaestloiktehley rfeedeudcctioonnssumipntfieoendwcaosnsduemtpetrimoinneodbtsoerbvee5d0tphrpomu,ghwhWileeekthe7,NtOheAELCOAwEasCdfeotrerremdiuncetdiotno bien: 10 ppm. Liver Weight: When compared to controls, there were no treatment-related effects on tlhiveerrewweaisghatsatamtoisntgicamlalylesibgonibfwihciatnte (qpua<i0l.n01)thtere1a0tmpenptm.retlraetaetdmeinntcr(eTaasbelein2)l.iveIrnwceoingthrtasti,n females from the 10 ppm treatment when compared to controls. Based on these result, bthaeseLdOoAnElCivefrowreaidguhltt wfaemsaldeetqeuramiilnsewda(s0bdeete1r0mpipnme.d o be 10 ppm. In males, the NOAEC Texapbolsee2d.o FeveadricouosnscuomncpetnitornatainondsobofdPyFaOnSd *liver weights of northern bobwhite quail "Treatment ADIT AvemgeFeed (pm) (my/kgday) Consumption wBeoigdhyt Liver weight (8 feedbird/day) Sex _(@ @ Control NA 1952 MFo 2240754:2106 48463654101510 10 07124006 19:3 MF20289:1:1 140498%4015650 50 2642024 9vs2 MFoo11g7s90t5s220 NAC 150 7324126 sre MO I87:14 NAC Valuewrsgiven sv eam nd sandard devine BoFdy1and 1ver9weiwge hermts 2mpe0paosurrrscetomnmetnertnoslt,s30wwidet1he0pcsopanmtmrpollgerdnoudapsW1ne0edpkWm7eaesnkdt6e5,3nrntesds3pefcostaimve5pl0lye.adndstF1e5Ws0ed/e2ck0onp2s1pa,mm.pwrrhieioscpnecddtaietvae$lwy0.assnfdor1W5e0e2k0 2 ANDAI Naovtersapgpelidcbilye innoaskaem(pmlegrPeFpoOrSt.gbodyweighty). + Atri indisicctasl significantdifference from he con vesment tp<0.01 4 cn07?9 GPrFoOsSs Praetlahtoeldogcyl:feNctescraompsoyngresquulatisloninaltlhesur1v0ivpinpgmfetmraelaetsmeinntdiwcahteend tchoampthaerreedwetroethneo rcoendturcoelsd.tesHtioswesivzeer,(6moaulteofqua1i6lmainletsh)eth1a0n pthpemcotnrteraotlmsen(t1 ohuatd oaf g1r6eamtaelres)i.nciAdlelncoethoerf findings were considered incidental and no relatedto treatment. Histopathology: There were no lesions in the liver, kidney, brain, proventriculus, apdpimpotsreeattimsesnute., orInbaudruslatfmaablrisc,usanininccirtehaesreadiunlttheorijnucviedneinlcee boofbswmhailtletqesutaeislssifzeroimn tthhee 1100 "pTphims sturgegatemsetnetd was that not accompanied PFOS might have by any morphological change acceleratedpostreproductive in spermatogenesis. regression, a normal physiological process. Based on these result, the LOAEC was determined to be 10 ppm, based on increased incidenceof small tests size. eRfefperctosduocntievgeg Rpersoudlutcst:ioWnhiennthceom1p0arpepdmtotrceoantmternotl.s, there were no However, while treatment-related not statistically significant, there were sight reductions in the number of viable embryos as a percentage oafseaggpesrecte,nhtaatgcehloinfgsha2t5c.h2lipnegsr.centSalgigehotfrleidvuec3t-iwonesckinemfebrrtiyloitsy,anhadticnha1b4i-ldiayyaonldd soufrfvsiprvionrgs assurvpievraclenatlasgoerseosfulbtoedthintrheeduncutmibonesroinftchgegnsumsebteranodf hthaetchmlaixngismaunmd n1u4m-bdaeyroolfd esgugrsvivseotr.s While slight, there was a statistically significant reduction in the number of 14-day old dseutrveirvmoirnsedasopebrece1n0tapgpems.ofthe numberof eggs set Based on this ffect, the LOAEC was AlidvuerltsaBmlpoloeds ashnodwLeidvetrhatPaFdOulSt Aqunaaillysiins:thRee1s0ulptpmofttrheeatcmheenmticgarlouapnaalcycsuimsuolfatbeldooPdFaOnSd ucornicnengtrtahteiosntsudmyea(sTuarbelde i3n).malAes tfhreomtetrhmein1a0tiponpmoftrtehaetmsetnutdyg,roluivperwearnedaspeprrouxmimPaFteOlSy s1p6e-c1i8fictidmiefsfegrreenacteerinthtainsstuheeccoonncceennttrraattiioonnss, mteheassuerreudm (0felmiavleers.ratWiohifloer btohterhesweaxsesawsaesx similar with a male ratio of 1.6 and a female ratio of 1.8. Since concentrations of PFOS bweerteweemneaPsFurOeSdexopnolsyurien aqnudaillivferroormsethreum10copnpcmenttrreaattmiewnotan,sanoqtuadneivietlaotipveed.relationship. 5 na780 s`Ttaubdlyet3e.rmMienaatnionc.oncAentrationsof PFOS in liver and serumofadult quail at 7 Treamem LverConc SemmConc. TT (Cpopmmo)l SeMxM (n<LgO)Q" <LO(g/QmL) F <10Q <L0Q 10 MF 848954221805 81741:52360 GmleayncsoannodlstaannddaTrOdpdomesvaimaostniwoewerntswmeliygzhetdbafsiosm dy. ATTvaTues ars repos "L00QgmLlimit ofquantitation; for ive twas 0.0502 e/g whit or serum i was sMteuadsyuirnedmiecnattedofthaPtFPOFSOSconaccecnutmrualtaitoensd iinn qbulaoilodincbooltlehcatetdimtehraonudghsoeuxtdtehpeenrdeepnrtodmuacntinoenr (Table 4). Tqaubaliel4. Concentrations of PFOS fig/mL) in serum collected from adult bobwhite Treament om) Sex 5 Samp1l0e time (week) 1s TCowol FoM 0000122:200000084 <0L00Q <<0 L0Q Q 10 Mo oes 158222 16451 F 8W4e2e2k8s SW6e3ek87 89:17 50 M 557 NA F 525 495 F*RLR0OQS=coLnimcietnoafqtuoanistawtieorn:eTpheoLwrsOtmQewemds 0s.n01d spngd. dovntioms HBoyweWveeerk, a5t, Wseereukm 1P0F,OsSexcosnpceecniftircatdiiofnfsedreindcensotindisffeerrubmetPwFeOeSn mcoanlceenatnrdatfieomnaslbeeqcuaaimle atphpaanretnhto.seThoebsceornvceedntirnatfieomnasleosf. PFMOoSreoinvemra,lethbisobtwrheintdecqounatiilnuweedretosiWgneiefikcan15t.lyFgermeaatleer b`moablwehsistaemqpulaeidl astetrhuemsaPmFeOStimceosn.cenTtrhaetidoencsrewaesreein18f-efmoallde lseessrutmhaPnFtOhSosceonocbesnetrrvaetdionins ciondiinccaitdeedthawtitfhemtahleeonqsueatiloftratnhsefeergrge-dlaPyFinOgSphiantsoe tihneitrheegsgtsudyd.uriTnhgisrepwrooudlucdtisone.em tIon bcoyntWreasetk, m1a0l,eansderthuemyPdiFdOnSotcosntacteinsttricaatliloyndsifrfeearfcohretdhaenraepmpaairnednetrsoafttuhraetisotnudcyo.ncentration Concentrations of PFOS were collected at each sampling time also measured (Table 5) in the red blood cels of blood samples 6 COn78L `Table 5. Concentrations quail exposed to various of PFOS in red blood cells concentrationsof PFOS A (g/mL) from adult bobwhite: Treatment Sex `Sample Period (week) (ppm) 5 10 is "Control TM <Q" <Q <q 10 F <10Q Mo 2672232 <1L0Q 1844325 <L0Q 35526 -- F 280+ 226 Weeks 74:82 Week? 20210 ~ 50 M 165 359 EF 106 110 "RPeL=bOlLoiodmQicetllofcqounacnetnittartaitoinoia:nnesrerd belopodoc3remtleetahnedsLaOnQd rwaansd0a.n0d1dyevgia,tions a`Tnhder1e5wbaust annotaaptpawreeenkt sS,exaldtihfofeurgehncteheirne risedsubblsotoandticaelllvmareiaabnilciotnyciennttrhaetidoantas.atRweedekblsoo10d wceelelkPsR1O0Stcoon15c.entTrhaetiorantsiodeocfresaesreudmitnobroetdhbmlaoloed acneldl fceomncaelnetroavteirontsimwe,erpearatliscoulsairmlyilfarroimn rmeadlebsloaonddceflelmsadleecslianteadllmotirmeetphoainnstserbuumt ltehveelrsbatyowsehiefkted15.over time as concentrations in vWehrislues stheerubmioilsougnicclaelars,igthneifbilcoanocdereosfultPsFsOhSowcotnhcaetnPtrFaOtiSonconccheanntgreastioinnsrdeedclbilnoeoddducreillnsg the reproductive phase. `CaonndcleinvterrastaimopnlessoffrPoFmOjuSveinnilBeloqouadilanidndiLciavteerd othfaJtuPveFnOiSlewQausaistli:llTphreesaennatlyisnitsohefsseeqruauiml 12 weeks increased after in the hatching. 10 ppm The PFOS concentrations in both treatment group over that measured in liver and serum were the control group, but (wTearbelele6s)s. tUhnalnikteheadcuolntcqeuntaria,tinoonssemxesapseucrifeidc diinffaedruelrtveqsuawielrefroobmsertvheedsianmjeuvterneialtemelinvtesr jourvesneirluemquPaFilOSavecronacgeendtra1t.i9o2ns+. The serum 0.46 and 1.97 to + liver 0.80, rraetsipoecftoirvePlyF.OSThien jmuavelneilaendsefreummalteo liver ratio was similar to the ratio observed in adult quail (males = 1.91; females = 1.42), 7 - CNa782 "jTuavbelneile6.quaCioln.centrations of PFOS in the liver and serumof 2 week old Treatment CoTnvceenrtPraFtOiSon CSoenrceunmtrPaRtiOoSn, CGonptr)ol sex M gl) <L0Q (ug/ml) <L0Q 10 MF 57<6L200Q67 12<6L503Q37 TTConceanhraons we reE poreands5a4nd9v%an1d1r4d Svions Do g1i2ve4n%3w4e6r L0G Limit ofquantitation; LOG for ve -20 ng/g: LOG for ser=u40m0 ng mEegagsuCroemdpionneegngts PcoFlOleSctCedonfcreonmtrtahteicoonnst:roPl FaOndS 1c0onpcepnmtrtarteiaotnmsenitnse,ggEgcgosmpwoenreentseslewcetreed PfrFoOmS.WeYeoklsk (1w9,ho2l0e,)2c1omapnodsiytoelskswewreerethceonmapnoasliytzeedd afnordPaFnaOlSyz(eTdabfloer7c)o.ncIenntardadtiitoinosnotfo pbeptmterturenadtemresnttanwdetrhee adilsstorisbeuptairoantoedf PiFnOoSthirneethefaecgtigonyso,lk,veyroylklsowcomdpenossiittyesliipnoptrhoete1i0n d(eVtLeDrLm)i,nedphinosevaictihn,yolaknfdracltiipoonv(iTtallbilne 7)f.ractions. PFOS. concentrations were then c`Taablyeol7k. cCoomnpcoennternattsio*ns of PFOS in bobwhite qual egg yolk and Treatment Fraction PROS CWoncentration "Co1n0tprpoml Yoa lk-whole 62:15 YoYloil-Pkh-oVsLviDtLin 038938 Ress gisvta enYoanldi-sLaindpeodvietveiltiinonsons WWI b1a32s The "duHpalincdaatesdefvrittoensxapmrensieqsuepsramepsles,oiafocthnopmrpeoofpssaeatvrenrsdaaalneta4li.yonof #1L0Q Limiofqantiaions LO=0Q.01 gs. eRegsgulytoslkisnodficnaotrethtehartnabtob1w0hpiptem,qutahielrseawsacsomapasirgendiftiocacnotntarcoclsu.muNlaotiPoFnOoSfwPaFsOdSetiencttoedthien agrqeuaatensttificaobnlceenctornacteinotnratiinonthien tyhoelkconwtarsol agsgsocyioaltke.d FwuirtthheVrmLoDreL, rfersauclttisons,hofwoeldlotwheadt tbhye lipovitelln. CONCLUSIONS: aNnodrt1h5e0m/2b0opbwphmitfeorquuapiltos 2w1erweeeekxsp.osDeduetotoPsFiOgnSs aotfdoiveetratrytocxiocnicteyn,taadauilotnqsuaoifl0i,n 1t0h,e 5500 and 150720 ppm treatments were terminated at the end of 7 and 5 weeks, respectively. 5 00783 f`eWehdencocnosmupmaprteidontoatcoantdroosles,ofthe1r0epwpemreinntohePFfOeSe-dr(eTlaabtleed8e)f.feHctosweovneard,ulmtibnoodryovweeritgshitgnosr soifgtnoixfiicciatnyt winecrreeaosbeseinrvfeedmainleadluilvtesr dwoesigehdt.at I1n0 papdmul.t mAatle1s0, ptphemr,e twhaersewanasinacrsetaasteistiincatlhley i1n0cipdepnmceotfrseamtamlelntesgtreosups.izewi(tThablreed8u).cedT.heteisntcerseassiezein wthaesnunmotbearcocfomadpualntimeadlebsyinatnhye amcocmehloerlaotgeidcalcarclhyang_peosti-nresppreordmucattiovgeenespihsa.se Trhegirsesssiuogng,estsa tnhaotrmaPlFOSphymsiaoyloghiacvale rpehleanteodmerneodnu.ctioAndsditiinonfaelrtliyl,ityt,hehraetwchearbeilstlyi,ghta,nbduat nosttatsitsattiicsatlilcyallsyigsniigfniicfainctanrtedturcetaitomnenti-n EoffffescptrinCgonscuernvtirvaalti(opn <(L0O.0A5E).C)BafosredPFonOSthreseepreofdfueccttsi,vetheeffLecotwsesitn bObosbewrhviatbelequaAidlvewrasse adsestoecrimaitneeddw1i0thbethe10LpowpemstinOfbeseedr,vabbalseedAodnve2r1seweEfefkescot fLeevxeplos(urLeO.AEPLF)OiSn bcoontcheandturlattiaonnds juvenile tissuesare summarized in Table 9. i`nTaablcehr8o.nMiecasstuudryemweintth ennodrptohienrtnsbaonbdwhaistseociqautaeildsdaincdtatrhyeNrOofAfspErinCg. and**LOAEC values for PFOS Endpoint Dietary NOAEC Dietary LOAEC MAoDrUtaLlTity 10 50 Body weight 10 50 LFieveedrcwoenisguhmtption Maks10= 10 Femal5e0s = 10 Gross pathology Females <10 Female=s 10 Mal=e5s0 Male=s 10 Histopathology Male<s 10 10 Females = 50 Reproductive 10 OF14F-SdaPy RsIuNrvGivabili 10 10ATpocmoncgernoturpatpioonsserdefopro21ormwteeewkedst3wpegmBgrAoFup expose (7owereks 9 650784 "cTharbolne.dLeOaArELstuvdayluweishinPvFarOiSous matics in alt andopfring northern bobwihite gus ina AMDeUasLT MofALPREOSS xpos LOL DASeoDrsIuem((mp(gpumgP)FPOFSO/Sk/gILb)odast swtuedyitegmihnavtteordn2(a12-ywwee)cekkpse)riod oa1rt0 ADLUiLveTr F(uEgMPAFLOSE/Sg) at study termination (21-weeks) 885 ADDosIe((mpgpmP) RkOgboSdy weighty)ovr21-week period o1m0 Serum (ug PFOS/mL), pre-reproductive phase (S-weeks) 84 Serum (ug PFOS/mL), reproductive phase, at study termination (21-weeks) 87 OFVLaFilSvkePrRu(IgiNgPGPFFOOSSi/mgL))at study termination (21-weeks) 49 @ Liver (ug PFOS/g) 55 "TTOceSRvieEornLnuomwSnaa(susukgbmPeoFaOncS/0me3Lb)rSn.astes WTyeaSroy r mtSeTmE eR a oT 1 r25 e -- op v afctp vios or ad ADI repr dar concetions. Scrum adverfc lcs OLafOfysApmEriLngvaailvueeirsafnordtomsraelpreusamirLenObAa,EseLd on histopathology, values ae versges forfemales on offemale snd increased liver|`weight, male concentrations. and on reduced 14- DReAlTaAbiQyU:ALKIliTmYi:sh rankin=g 1 REFERENCES: DqBueeacmikalerredg,tEayRnold,k.CFolEoaxh.yergt1ey9n,60RL.eMpB.oBrtA20N04o34.,:08A26n5.a9.l60y,7si0s of pecuoroocine sulfonate in mallard and Dwuintnhectto,ntCoWl.. J1A95m5.esA Smtlatis.pAescsoonp5a0i:so1n09p.r1o1c2ed1ure for comparing several rcatments D0un0ne,1C:W. 1968. New tables for mulipl comparisons with a control Biometrics 10 (8S Gsatluldaygwhiert,h tSh.Pe.,NoVratnheHronvBeonb,whRi.Lt.e. BFeianvaelrsr,ep1o.rBt.., JWaibledri,ifMe.I2n0t0e3m.atiPonFaOlS,: A Ltd., rePprroojdeuctctNioo.n 454-108, GCeallllsaghaenrd, SSe.r2u0m03.forEAxtnraalcytsiiosn uosfinPgotHaPsLsCi-umElPeecrtfrlousoprrooacya/tMnaesssulSfpoencattreofmertormy.RedExBylgoeond Research, Exygen Study Number 023-066. Reagen, WK. Yolk. Exygen R2e0s0e2a.rchA.naElxyysgiesnoRfepPoerrtflNuoo.ro0o2c3t-a0n7e0s.ulfonate in Mallard and Quail Egg SNtoervtehnesorn,BoLb.wAh.it2e0.03.3MAnEanlvyitriocnamlenpthaasleLraebpoorrattoforry PEOS: Study A No. reproduction EOI-1245. study with the S~ticfoagnin,at.e, lNigiamndpsfJa,.n,dSocohcnyetiederre,ceWp.tJo.rs1-9t9h0e.biVnidteilnlgosgietneesfiosr viintXeellnoogpenuisn liaselvoicsaatnedd ochnicken lipovitelin-1. BIOL CHEM 265: 882-885. `Wildlife Intemational, Ltd. 1994. Avian Reproductive Data System (ARDS), Version 2. OTHER Last changed: 5/05/04 n C0786