Document nNRy0x2p3z7yqBz42qenpenM1

AR226-3175 DuPont-6010 TRADESECRET Study TUUs H-24516: Static, Acute, 48-Hour Screening Test to Daphnia magna LaboratoryProject ID: DuPont-6010 A u th o r : Robert A. Hoke, Ph.D. Stu d y C o m p l e t e d o n : March 12,2001 P er fo r m in g L a b o r a to r y : EH.aI.skdeullPLoanbtodreaNtoermy foourrTsoaxnidcoCloomgypaanndyIndustrial Medicine 1090 Elkton Road, P.O.Box 50 Newark, Delaware 19714-0050 -1- Cmpany Sanitized, Does no? contain TSCA CBI H-24516: Static, Acute, 48-Hour Screening Test to Daphnia magna Haskell Number 24516 DuPont-6010 Physical Characteristics: Pale to medium tan waxy solid Stability: Tcohnedtietisotnssubosfttahnecestaupdpye;anroedevtoidbeencsetaoblfeinusntadberilditiyewas observed. Solubility: Negligible in water H-24516: Screening Static, Test to ADcaupthen, 4ia8m-Haogunra Sponsor: E. I. du Pont de Nemours and Company Wilmington, Delaware 19898 U.S.A. Study Initiated/Completed: February20,2001/March 12,2001 In-Life Initiated/Completed: February20,2001/Fbruaiy 22,2001 DuPont-6010 -3 - Corrpany Sanitized. Does not contain TSCA CB1 H-24516: Static, Acute, 48-Hour Screening Test to Daphnia magna DuPont-6010 SUMMARY The acute toxicity o fH-24516 to the water flea, Daphnia magna (less than 24 hours old), was determined in an unaerated, 48-hour, static test The studywas conducted with 4 concentrations ofH-24516 and a dilution water control at a mccooennactnreontletrmaanptdieoranalltwuteirtsehtoc1fo02nt0cee.s0nttCorragt(iraoannnissgmwe oserifne1ce9la.e9ca-hr2c0ahn.1admCcob).leorOr. lenBseastsweesditthochnuavnmidsbiuseasrolwlovbaessdeurtesvesadttimopnearste,trtehisatel vwiasitberle in all test chambers except toe water control at test start. All water quality parameters were within acceptable limits during toe exposure. Exposure ofdaphnids to toe water control and nominal H-24516 concentrations of 1,10,100, and 1000 mg/L resulted in 10,10,0,30, and 50% immobility, respectively, at toe end o f48 hours. Nominal H-24516 concentrations were used for calculation ofEC50 values. The results are summarized as follows: Nominal concentrations ofH-24516, mg/L 48-hour ECso (95% confidence interval) dilution water control, 1,10,100, and 1000 965 (576 to 3139) for H-24516, based on nominal concentrations, mg/L8 H-24516 exhibited low concern for aquatic hazardbin an unaerated, 48-hour, static acute test using the water flea, Daphnia magna (less than 24 hours old). a SAS User's Guide, The PROBITProcedure, SAS Institute Inc., Cary, North Carolina, pp. 639 - 646. b m(SWmet.rhGcoh.deTsk--,aJnd.d,fcCevlJea.mSlu.eaHntituosg,nRhoe.fs,,dMaantoadr.cMoEcn.kAv,.iRrLo-e,nwamniesdn,REtaadlbsTe.)or,txp,iWpco2.lo2(g1-3y999a.3n)Ad. RmAiessrksiecAsassnsineSgsosecmcieeotnlyot,fgoAicrSaTTlehMsatizSnaTgrPda1nu1dn7dM9e.raTteSrCiaAls,: Philadelphia. -4 - .Company Sanitized. Does not contain TSCA CB1 H-24516: Screening Static, Test to ADcaupthen, 4ia8-mHaogunra DuPont-6010 CERTIFICATION We, tiie undersigned, declare thatthis reportprovides an accurate evaluation of data obtained from this study. Work Completed by: ^ * ** ** * 7 Daniel J. Peterson, B.S. Associate Scientist / i - /W /9 S -c/t - 0 0 / D ate Issued by Study Director: ^6>u/- _______ Robert A. H ols, PhJD. SeniorResearch Scientist /c2 Afandt dto&/ D ate -5 - Company Sanitized. Does not contain t ^ a oqj H-24516: Screening Static, Test to ADcaupthen, 4ia8-mHaogunra DuPont-6010 TABLE 1 DISSOLVED OXYGEN AND pH OF TEST AND CONTROL SOLUTION SAMPLES Nominal H-24516 Concentration (mg/L) Test Initiation (0 hours) Dissolved Oxygen (mg/L) pH Test Completion (48 hours) Dissolved Oxygen (mg/L) PH_____ Water Control 1 10 100 1000 88..88 88..87 8.7 7.4 7.4 77..54 7.3 8.7 88..88 88..88 7.5 77..56 7.6 7.5 TABLE 2 IMMOBILITY OF Daphnia magna, IN AN UNAERATED, STATIC, ACUTE, 48-HOUR TEST WITH H-24516 Nominal CoHnc-e2n4t5r1a6tion (mg/L) Water Control 1 10 1100000 Number Immobile / 24 Number Hours at Studv 4S8taHrtours 00//1100 001///111000 1/10 3051////11110000 r'Company Sanitized. Does not contain TSC/*