Document nNQkGgBv2w7R1eMq4L8RR0N4m

Wia.6-ozod ACUTE TO XIC ITY TO AQUATIC INVERTEBRATES (DAPHNIA MAGNA) TEST SUBSTANCE_________________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-206CE. (1Octanesulfonic acid) (CAS # 2795-39-3). Rem arks: The 3M production lot number was 502. The test sample is FC206CE (2/16/89). Current information indicates it is a mixture of 0.92% PFOS, 15% diethylene glycol butyl ether, 76.09% water, 4% urea, 1.28% Sultone foamer, 1.55% sodium octyl sulfate, 1.05% polyoxyethylene monooctylphenyl ether, 0.06% sodium lauryl sulfate, and 0.05% tolytriazole. The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect the toxicity o f the fluorochemical component o f the test sample. M E T H O D :________________________________________________________ M ethod: OECD Method 202 and U.S. EPA Method EG-1 Type: Acute static GLP: No Y ear com pleted: 1989 Species: Daphniamagna Supplier: In-house cultures. Initial brood stock from Aquatic Bio Systems, Inc, Fort Collins, CO. A nalytical m onitoring: Temperature, pH, and DO Exposure period: 48-hours T est organism age: <24 hours old S tatistical m ethod: Not given. Test conditions: Dilution w ater: Carbon-filtered well water Dilution w ater chem istry: Tem perature: 20 C Dissolved oxygen: 9.6 mg/L pH: 8.0 Lighting: Not given. Stock and test solution preparation: The test material was added directly to the dilution water on a weight/volume basis. Exposure vessels: 250 mL Pyrex glass beakers containing 200 mL of test----solution and a water depth of 6 cm. Num ber o f replicates: 2 Num ber o f daphnids per replicate: 10 Num ber o f concentrations: one plus a blank control W ater chem istry during the study: oor *v pH range (0-48 hours): 8.0 - 8.2 (control exposure) 7.9 - 8.1 (1000 mg/L exposure) Tem perature range (0 4 8 hours): 20 - 21 C Dissolved oxygen range (0 4 8 hours): 8.5 - 9.6 mg/L (control exposure) 4.5 - 9.6 mg/L (1000 mg/L exposure) R E S U L T S ____________________________________________________ _ Nom inal concentrations: Blank control and 1000 mg/L Elem ent values: 48-hour EC50 = >1.000 mg/L Element values based on nominal concentrations Rem arks: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. C O N C L U S IO N S __________________________________________________ The FC-206CE 48-hour EC50 was determined to be >1000 mg/L. Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY___________________________________________________ R eliability: Klimisch ranking 2. Testing meets the criteria for quality testing. However, sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. R E F E R E N C E S ___________________________________________________ This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number G 1 3 5 8 -2 ,1989. O T H E R __________________________________________________________ Last changed: 6/26/00 001734 ENVIRONMENTAL LABORATORY DAPHNIA TOXICITY DATA SHEET LR TEST TYPE, CHEMICAL TESTED FC Ctifi C<. TUT TEST ORGANIC!rjklph/tirt. SCIJRCET^y^^^^ysA^ir^i^/^i^k^/kDATE RETJD AGE OF TEST QRGANI34S:<2i hrs. old instars (neonates). NO. ORGANISMS PER TREATMENT.//? TEST VESSEL: 250 ml PvrexR glass beaker TEST SOLUTION VOLUME: 2QQ.JlLW.ATER DEPTH: Juxi_ TIME STARTED.__ \\nnpn DATE STARTED, S /u /M __ DATE COMPLETED DILUTION WATER Carbon-filtered well .water fTemp. 2D_2L D.o.__iz_mgZIu pH.^7. .) ANALYST^ fp A f/fr EC5Qf95* Confidence Limits)-- OOP nHj/U-------- -- . Test Initial Total 24 Hours 48 Hours Vessel Cone. Amount Total No. pH D.O. Testai No, pH D.O. No. mg/L Added wiq 3 X Control Dead r> mg/L Dead mg/L O ftf, f t t f iVitt4rol *r 1 innn znn O O n o li ItYY) ZiY).. O o Test Tems. C Initials Date Z\ "K5 ^ //z /0 9 . " ^ 3 S //3 /8 9 Cannents: ly M ) Xq. T .< im^tW UP rle flry lh u ^ usH-U aiafipr. IvttHafl ipH- t ------------ . Test methodology conforms with the following two test protocols: (1) OECD 202 "Daphnia sp., Acute Immobilization Test and Reproduction Test". (2) US-EPA #BG-1 "Daphnid Acute Toxicity Test". 001735