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AR226-2931 Haskell Laboratory fteport No. 802-85 Haskell E, I. du Pont de Nemours and Company Laboratory for Toxicology and Industrial P. 0. Box 50. Elkton Road- Newark, Delaware 19714 Medicine D&te Issued: January 6, 1986: Company Sanitized. Ooe not contain TSCA COS g"? s^ oupsot-6 male Crl;](^Sp^(|yp^^ to aerosol atmospheres forASfriale,-- fcL- UoNec^e conditions of this forr s greater tJh.1a.-n... l1a8H0U0V n-^ig./_/in) , &thLe_ LhJig-Lh-e.sJt. _ concentration tha^TBUld be^geperated. ThIs-iiM^ertal Is considered no worse than slightly toxic on an acute inhalation basis* york by: Ha^f TM^^ T Robert T. Turner Technician ^ /^ /P6 Reviewed by: lOfflas A* Kegftunan Technician Study Director j-ows^ fi ' ^^~> Laura A, Kinney- Chemist * / 3 / 8^. Approved by: ncy v Chromey, Pipo. // &/f& Seetlon Supervisor, Acute Investigations Section LAK;smi':HLR17.17 - 2- Company Sanfteed. Doe, not contain TSCA CBS j Textile fibers Defrtment ! E. { dd^ Pont^itpHonours and Coapany Wnfflfngton, OeTaitffe Material Submitted By: L^xtTirT5b5?rS(e^rtnent i * I du (^^^lefliours and Company ; Seaford. Del^lf^ Test Facility: E. I. du Pont de ftefflours and Company Hastell Laboratory for Toxicology and Industrial Medicine P. 0. Box 50, EUtwi Road Newark, Delaware 19714 Study Initiated - Completed: 12/7/84 - 12/26/84 There are 7 pages in this report. Distribution; - 3 - Company Sanitized. Does not contain T5CA CBI  ;.,'- ";.^ ;;-,"^^fe;~-'""""'.'^ -- '-'y^-'-^ i:^.^.'"' S&3V..:,'vf^ of.tW^til^j^^^a^^ The purpose Inhalattoe AtC for ^tN^W*^ l^ulatim^^e ---J^M^-iMl^-rais^'^r'.^WirNp^ lowest atoosphe^c . concentraffort fcesitfld^rt saos it '-wlwrc rats either oo Use day of exposure or wi$h^a;l4 (ti^^po^ exi^iure.^^^^!^ as documented in the study records, this sfuiftf was ;<?n<(ucted uccordffl^ to the applicable Gootf Laboratory Practice validity of this study. ofMthe deviations noted affected the 1 ^ ' s ^.^ MATERIALS AW) METHODS A. Anima] Husbandry young adult male Crl;C^(SO)BRrats were received froa Charles River Breeding Laboratories. Kingston. NCT York. Each rat was assigned a unique 6-dlgft Identification number which corresponded to a numbered card affixed to She cage. 'Rats were quaranttned for one week prior tc testing, and were Wslgned iand observed twice during the quarantine period. During the test, rats were housed la pairs In 8'' x 14" K 8" suspended, stainless steel;, wire-mesh cages. The rat assigned the lower number In each cage was Idjentifled by a slash in the right ear* Prior to exposure, rats' tails and (cage cards were color-coded with waterInsoluble markers so that individual rats could be identified after exposure* Except during exposure, Purlna Certified Rodent ChoW rSOO? and water were avail able ssl Ubituni. B. Exposure Protocol ; Groups of 6 rats, 8 wejeks old and weighing betwe ;n ?27 and 293 yvi-^. were restrained in perforated, stainless steel cyHr;ers with conical nose pieces. Each group ws eitposad nose-on*yfor < single, A-hn-- period to an aerosol atroosphere olHrt^--ftIp air . ":!.- -;:.-,- ceigded prior to exposure, and were obserwiFTorcnnTcal s'^ns of toxictty during exposure. For 2 exposures, surviving rat.'- -are weighed and observed dally for 14 days;'post exposure* weekends and holidays excluded except when warranted by the rats1 condition. For 2 exposures, rats were weighed and observed on tha !5th er 15th day Of recovery because the 14th day fell on a holiday, C. Test Material Physical Form; cow^^^^ T^i^ - 4- Purity; Composition: Contaminants; Synonyias: Stability: 0. Atmosphere Generation Aerosol atmospheres o liquid &sfc safcenal to a re generated by pimping the liayiftg_ _ Systems nebulizer. Air Introduced at the nebulizer aerosolized e test material and swept the aerosol stream l^lRi^s In the 38-Uter glass exposure chaiBber. the. atmospheric concentration of controlled by varying the liquid and air now rates. Th^chamber exhaust twis drai^-fhroagh s cold trap and a MSA cartridge filter prior to being dischac'ged Into the hooa . Analytical ou--Wfjwas The atmospheric concceennttrr^attli-on ffHI determined at approxfimately 30-mlnute inte^rvamls durTfnfg each exposure by gravimetric analysis. Calibrated volmaei of chaB&er atBO'spnere were drawn from the rats' breathing zone. through'prewlghedfietmaH* glass fiber filters. Filters were dried overnlglittlh s desiccator containing Drierfte* to remove residual water from the sample. Filters were weighed on a Cahn ?! 26 automatic tlectroba|anee. The atao?fher1c concentration of Ul^yblymer was calculated fron the difference between the pre post-sampling weights ofithedried filters. ! Particle size (mass median aerodynamic diameter and percent . resplrable) was deterelned wtth a Sierra Series 210 cascade Impactor during each exposure. During each exposure, chamber temperature was measured with a mercury therihometer and relative humidity was measured with a Bendix Model 566 psychrometer. Ouring Bost exposures, chamber oxygen content "as measured ffth a BioHarine Model 2?5 oxygen analyzer. Con, PaflyS,^"^e^ooe, SROS^^ A. Exposure Conditions and associated Mortal fty During exposure^ chaiBl^ tefflperatupe ranged^between 21-22C, relative humidity ranged from 28-S%,| (Hid chamber o^ygeflElcontent was 211, Atmospheric characterization and associated rat mortality data are sumnarlzed below, I Character!zatftm oftlHWlliyAtmosplteres 'and".teAeiated Rat 14&f^ailj|y a Part iculate Coneentratf on fnig/ro3) Mean ^.D. Range ! 35It0 710 1800 17 0 - 43 100 250 - 550 64 620 - 790 150 1600 - 2000 i Resplrable j . ?55 96 95 83 Mortality MMOfunt)0 if deaths/f exposed) ^ u/6 2.4 0/6 2;2^^ 0/6 4.4 0/6 . Represents the concent rat ijpns of active ingredient onTy (water excluded). . Percent by weight of parti;cles with aerodynamic diameter less than 10 MB. l" Mass median aerodynamic dijameter. r No additional exposures jto higher concentrations were attempted because the 1800 fflg/m exposure concentration approached the 'limits of the generation system. B. Clinical Observations iditlons of fchis study, no rats died following exp&syrs Ouring or 1ffi(iiediate1y fQllowfng exposure^ rats in all 6 Co; ^ys,,^ ^ff^. not ^^Tsc^ a^"??,- fioiaagw grouns had a lag/a*-.rats* rats exposed exposure, red nasal faces Mn" to 350 is greater than 17 lal. Jn addition, response during During the posti had slight weight les^ day after exposure. 1^1; 17. 350 or 710 ag/<r tial body weight) 1 Rats exposed to 1809 ng/n lost an a^erage^ 1 day after exposure, and 2 rats C6h^tiroed^<t No adxerse clinical {.were observed in 10 mg/nr. At 1800 mg/ro . some rats 'had .faces' stiiNii^^^J^^st^iBKffienal, hair loss froa the body. dry red nasal disehirge, broiaci-staift^ perineiCTi and ruffled fur at various times during toe nir^ 9^<aay^after^^^ -COSiClOSISN Under the conditions of this study, the ALC fof^itfHMll|**as greater than 1800 mg/m', thehlgnestcoffc^fratiwtttatcot^ generated. This material Is considered no worse than-slighTy toxic on an acute inhalation basis (ALC betwen 800 and 2000 ffigi/TO-). 1 Calculation described in Sierra iInstruBients. Inc., Bulletin 7-79-219IM. Instruction Manual: Series 210jAnbient Cascade tmpaetors and Cyclone PreseparaioF?!''"""'-.--"" aom^^^ - 7 - ^^can^'"^CAOBf