Document nBv0RRaLJNw5k0BqwmJGNw66
AR R26 -- 0737
BIODEGRADATION (301E)
TEST SUBSTANCE
Identity: ArefmeirxrteudretocaonstPaiFnOiSng, pFeCr-f9l5u,oroorocatsanaescuolmfponoanteen,twhoficFhC-m2a0y3aAlsoro obfe FC-203 NFP. (1-Octanesulfonic acid) (CAS # 2795-39-3).
Remarks: is FC-203
The 3M production lot NFP Current information
numberwas not noted. indicates it is a mixture
The test of 1.34%
sample PFOS,
25% diethylene glycol butyl ether, 67.85% water, sodium octyl sulfate, 0.1% sodium lauryl sulfate,
2.66 % Sulton foamer, and 0.05% tolyltriazole.
3%
cThhaerafcotlelrowiiznegd scounmcmeantrryataipoplniseosftiompaurmiitxiteusr.e Dwaittha imnacyomnpoltetaeclcyurately reflect degradation potential of the fluorochemical component of the
test sample.
METHOD
Method: Modified OECD Screening Test, OECD 301E, with DOC Analysis,
1981 version. Test type: Ready
Biodegradabilty
GYeLaPr:CoNmopleted: 1982
AStnaatliysttiiccaall
mmoentihtoodrsi:ng:ResDuilstssolwveerdeodregatneircmicnaerdbobny
(DOC) calculation
of
the
%
DOC
Treemsotvoarlgaanndisgmraspohiucrcien:terApre5t0a:t5i0onmix of soil extract and secondary effluent.
TaehreatsieocnobnadsairnyaetftflhueenMtetwraosWtahsetseuwpaetrenratTarnetaftrmoemntanPlaacntt,ivSatt.edPasullu,dMgNe, while
the soil was from the CityofWhite Bear Lake, Ramsey County, MN.
Test condition:
Dilution Mineral
water: Deionized Nutrient Medium:
water Nutrient
medium
per
OECD
301E
method
--
(1981). Initial pH 7.0. Reference andtestsolution
preparation:
The test material was
Tphriespasroeldutbioyn
dgiisvseoslvainfgin1al53tesmtgcoinnctewnotrlaitteirosnooffm2in0ermagl
nutrient DOCIL.
medium. The
r3e0f.e3rmengceinsoubnsetalintcere,ofsmoidnieuramlbneuntzroiaentte,mewdaisump.repTahriesdsoblyutdiiosnsoglivviensg a
final test concentration of 20 mg DOCIL. Test vessels: Not given
-
Incubation conditions: Constant dark conditions
Temperature: Agitation:
23.5-24C Continuously
Number of concentrations: 1 plus sodium benzoate (reference
substance) and blank, all in duplicate.
000910
EInloecmuelnutmBacsoinsd:itiDoencroenasteesitnidniistisaotlivoend: oNrogtagniivcecnar. bon compared to Ttheestblsaunbkss.tance flask conditions: Not given.
RESULTS
eee
aNnodmianta~l2c0omncgenDtOrCaItLiopnlsu:s HBglaCnLk_c(oinnthriobli,tesdo),diteusmt bmeatnezroiaatleatat~~2200mmggDDOCOICLI.L
and at ~20 mg DOCI/L plus HgClz, all in duplicate.
Element values: 27-day Degradation = 76.7% duplicate 1and 81.3%
duplicate 2. Mean value = 79%
SRuebmsatraknsc:eTReesmtianrgkwsafsielcd.onTduhcetveadluoenstrheepmoirxtteudraepapslydetosctrhiabtemdixitnutrheeaTnedstnot
the fluorochemical proportion alone.
CONCLUSIONS
The FC-203 NFP % degradation based on the mean DOC removal value was
79 after 27 days.
Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St.
Paul, Minnesota, 55133
DATA QUALITY Reliability: Kimisch ranking = 2. This study meets the criteria for quality tsetsutidnyg.lacHkoswaenvaelryt,ictahlecsoanfmiprlmeatpiuornitoyfwtahse anmotouprnotpeorflfylucohraorcahcetremriiczaeldparonpdortthieon
in the solution.
REFERENCES
TSth.ePasutlu,diMeNs,weLraebcRoenqduuecsttednubmybtehre 384M83C,om19p8a2n.y, Environmental Laboratory,
OTHER
OO
Last changed: 6/28/00
000911
3m |Fomenrara
TECHNICAL REPORT SUMMARY
TO: TECHNICAL COMMUNICATIONS CENTER -- 201-20
important Ifreportsprintedon both sides ofpaper,sandtwocopiets0 TCC.)
6/30/80
wrEnvironmental Laboratory (EE & PC) Commercial Chemicals Division
re 0535 9970012600
Biodegradation of "LIGHT WATER" Products in OECD Test-6/80 | 040
John A. Pignato - Commercial Chemicals Division - 236-24 (01)
Eric A. Reiner - Environmental Lab (E&PC) - 21-BW (63) 47816
None -- See 3M Environmental Lab Request No. 5541S
[Pr
T cores
ena,
31 Tre
eO ronosR am a
Is Soeother
ELnavbiorroantmoreyntal (EE & PC)
CoRRENT GRECTIVE: TToestusewitthheDOiCntearnnaaltyisoinsaltloy shroewcogthnaitzed"LOIEGCHDT WSAcTrEeRe"ning products are highly biodegradable.
AFFF
S EPA O ABTSTRAACT. (2R00R750wad ois vat formations Gnade TocCammanetnsCorer 0
Toc
This study used the "Modified OECD Screening Test with DOC
cAnoanlcylsuissi"velaynddesmuopnpsltermaetnetalthepaerxatlleenlsivseterbiiloedegcroandtarbolislittyo of
"FCL-I3G0H1T7.WATEIRn" 14Bradanyds,AFFtFhepdroidsuscotlsv:ed oFrCg-a20n3i,c cFCa-r2b0o6n, (aDnOCd)
ldeegvrealdsedo.f FCF-C2-02063desghrowaedded93b%y D9O0%C daengdraFdCa-t3i01o7n wians2194%days.
nTohte dpuaeraltloelchesmtiecrailleorcopnhtyrsoilcsalprporvoecdestsheast stuhcish aDsOC loss was
maadtseorripatli.on, volatilization, or precipitation of the pare2nt
format gon
" mSRan
000912
EN
2-
BIODEGRADATIONIONFOE"CLDIGTHETSTWATER" PRODUCTS
INTRODUCTION
Tbhieode3g4raEdnvaibriolnimteyntoafl "LLaIbGoHrTatWoArTyER"chopsreodutcotsattuesmipntg ttohedeOmEoCnDstsrcarteeenitnhge mEentvhiordonmbeenctaaulseLathbe hatsesthadhasexwpiedreienicneterunsaitnigontahle OaEcCcDepttaenscte,4sanad the pCaormtpilceimpeannttthien aenxisitnitnegrnaBtOiDondaaltaritnhgattesshto.w tThheesseeprreosduulcttss wtiollhave a high degree of biodegradability.
TchaerboOnE.CD Ftoerstthiseserunwatater-2m0i-s2c5i9Cbleand"LIitGHmTeaWsAuTErRe"s opnrloyducdtiss,solwvheidchoragraenic ntoetsteinxgp,ecttehde OtEoCDvoltaetsitliizse,anpraepcpirpoiptraitaet,e morethbeodadfsoorrbmeedasudurriinngg total pdeogrrtaidoantioofn.theTsehepTr0oCducatnsa.lysis method even measures the perfluorinated
METHODS AND MATERIALS
FMetoholds lantodheCwoSneidpittneimogbnesr- 1W9,ade197A9., ScvheerislionpeorffortmheedMotdhiisfietdestOiEnCgD aSctrteaecnhiendg (TAepsptenwdiitxh 1D).OC Analysis{1). A copy of this method is
Raevdauicltaibolnesshinakithneg secqauliepmeonft.theFtievsethwuenrderedn-emclessEarrlyenmtoeyeardapftlasitksto rreeppllaacceedd 920-0limtle,r afnldasktsh,e s25a0mplmle osfizeinwoacsula2t5,ednontutr30ienmlt.solAustiionnthe pErielsecrraitbeedforproDcOeCduarnea,lystihse. analyst used only the last 10 ml of sample
wAictahliinbrtahteed20c-h2a5r0tC rreacngoerdedrurimnognittohreed28t-hdeaytetmespte,rateuxrceeptwhifcohr sata3y-e4dhr. pheerairodthwehehnighiterndeacohfedthe25.r5a0nCg.e (T2h3e.5-t2e4m5pCe)r.ature was most frequently
Chemicals - All chemicals were reagent grade unless otherwise noted.
oTfheAn3tMwe"rLpIGmHaTnuWfAaTcERt"urep.rodTuchtesirulseadbelweriedenstaimfpileeds tofhemcoamsmerFcCi-a2l03mNaEtPe,rial FC-3017 Lot 2303, and FC-206 (FH 3824) Lot 2330 10/79.
gThreadehydhryodqruoiqnuoinneoneuse9d8.5a%s, thLeotca1l0i01b4r7a.tionFoucrompcohuenmdicawlas sAulpdprliychhourseeasgent wperreescrciobnetdactbeydthien ManodiaftiteedmptOECtoD SocbrteaeinnintgheTe9s9t.,5%bupturethhisydrpouqruiitnyonies rapepdaurceendtypunroittyrweialdliyhaavvaeilnaoblseigninfictahentUSeAf.fectWeonexpreecstulttsh.at Tthheis tihnepiurritpireessenhcaeve conouldappmaakreentonliynhaibittroirvyialeffcehcatngeon imnicTrOoCormgeaansiusrmemsenatnsd.
000913
.
-3-
WwaasterSt.usePdaulincitthye pwarteeprarapatsisoendoftharlolughsoalutciaornbso,n ebexdc,eptmactrhoepoirnooucsulum, atnhiroonuigch ra es0i.n2-ubemd,filatnder.2 miTxheids wbaedterdeiwoasnizaelrsso, usaendd ttohenmakfeilutpereldosses etxopeevraipmoernattieonns.uredStcoocknssiosftenwtatewratecrolqlueacltietdy.at the start of the
Itnhepprrepeasrcirnigbednut3r3.i4entg osfolNutHipoHnPsO,422H6,.04, g3O6f.3agnhyOfdroCuasCNlya-H2PHO0, rerpelpalcaecedd
2i7d.e5ntigcaClaCltyo. thTohseesereqrueiprleadcembeynttsheyOiEeCldDedMetshooldu.tionTsheaondpticoonnaclentyreaatsitons
e(x1t.r6a.c1t.2(so)l)u.tionThewas100usemgd
in of
pFlea-ccehelOfatetheusevditaimninthesoltruatcieonelement
solution consisted of equimolar amounts of FeCl and EDTA (30 mg FeCly
and 70 mg EDTA).
TSeesrtvedConascentthreatbiaosniss -forDOCadmjeuasstuinrgemeinntistiaolf staemsptlecosntcoecnktrdaitliuotnisontso acpopnrcoexnitmraatteiloyn 4i0n mtgheofracngaerbornecpoemrmenlidteedr.by TthhiesOEisCDthteesth.ighe-Usste of this hmiegahsurienmietnitasl.conTcheentlroawtitoonxiciintcyreaosfed"LtIhGeHTsWeAnTsEiRt"iviptryodoufcts% DtOoC removal muinclirkoeolryg.anisms made significant inhibition at this concentration
Acadrjbuosntmepnetr olfitehrydrwoagsuidnoonnee,bythweeicghatlibbraastediononcoimtpsouknndo,wntocar2b0onmg of concentration of 65.4%.
Tcwoorrebcltainoknsc.ontrols, instead of 1, were utilized in making blank DOC
IT.n6o.c2u.l1uman-dTh1e.6.i2n.o2cuolfumtwhaesOE5C0D-50temstixtuusriengprferpeasrhedsoaiclcofrrdoimngE.toA. steps Rseliundegre'saegraartdieonn abnadsintheatsutpheernSat.tanPtaulofMaetrsoampPlleantf.romThaen afcitlitveartepdaper cusheldorwianse-Wfrheaetmwaenll54w,atearn.d the water for soil extraction was
S3stersitleerilCeontcroonltsrol-sRefpolriceaatceh vteessstelmsatecroinatlainainndg th4e00cmagl/ilbraHtCiloyn serv--ed choamnpdoluinndg. ofHatnhdelivnigabolfe cthuelsteuresst.erilTeheyconhtardoltshewassameideinntnioccaullumtoatnhde s0-ampalnidng28f-rdeaqyuesnacmyp,lesb.ut the analyst only made DOC measurements on the
Sample Preservation - The sample preservation method used was that pdrreospcrofibecdoncbeynttrhaeteTdOC HCmlanutfoactthuere1r0(4m)l. fiTlhteermeedthsoadmpliensv,olbversinagdidnigngthe1 wsiatmhplealutmoin<upmH 2,0ialn-dlinsetdoricnagps.theThseampmleetshodundiesranreeffrfiegcetriavteion in vials pstraebsielrivzaetiocnalimebtrhaotdi.on Tshoelutmiaonnusfacmtaduererfroimndpicoattaesssiutmhathydtrheogemnethod can phthalate, a readily biodegradable material, for several months.
000914
"t=
Sniencceessatrhye astceipdifiincattheionTOCinantahliyssipsrepsreortvoactoilo,n mietsthoudsewwaassatlhseo maost pdresaccrtiibceadl ainndthaelloOwEeCdD emeltihmoidn.atioAlnloTfOCtheanaHlgCylspespwreerseervmaatdeionwittheicnhniaque month of the preservation of the samples.
DIonhsrtmraunmenDtCs-52aAn.d
eqIutispmseenntsi-tiTvhietyorlgiamniitc icsarbbeotnweeannal1yzaendr
used 2 mg
was a of carbon
per liter.
Tphleugss.toppGeerlsmanfor0.2theumrmeeamcbtiroanneflfaislktsersw,ere(PcalretanN,o. po6r4o8u1s4,) bpolialsetdic3foam tines and stored in deionized water, served to filter samples for DOC analysis.
G(lCahsrsowmaerregeRc)leafnoilnlgoweidnvobylve6ddesiooankiinzgedinwatcehrromriicnseasc.id cleaning solution
RESULTS AND DISCUSSION
Tcaobnlteain1 sthuemmaarcitzueasl tdhaetaresshuelettss oafndthpilsotsstuodfy.theApdpeegnrdaidcaetsion1 aansda2 cfoumnpclteitonelyo.f time. ALL 3 "LIGHT WATER" AFFF products degraded nearly
Percent
Degradation
of
TABLE Hydroguinone
1 and
"LIGHT
WATER"
AFFF
Products
FC-203,ModFiC-f2i0e6d, OEanCdD FSCc-r3e0e1n7ingWitThestTime in
+ Degradation at Day:
Product 1 14
21
21
28
FFCC--220036 2133 9805
9934
9931
9923
FHCy-d3r0o1-7 8397 9924
9976
9964
9953
-
quinone
vIenrytheunclaiskeeloyf tthhaet
hpihgyhsliycalwatmeeranssolsuubclhe as"LIaGdHsTorWpAtTiEoRn",
products, it is volatilization,
osrubpsrteacnitpiiattaetdiobny ctaheuserdelathteivellosyshoifghsoolxuybgleen TdOeCman(dDOCo)b.servTheids inisBOD
tests of the 3M Environmental Laboratory (FC-203 BODs/COD = 0.5, =
FC-206 BOD5/COD = 0.5).
TehveidesntceeriltehatcontthreolsodlautbaleinTOCTablloess2fprroomvitdhee ftuerstthesrampslubesstacnotnitaalining p"rLeIcGiHpTitWaAtTiEoRn". proTdhuecstes saismpnloets wdueeretohaanddsloerdptiidoenn,tivcoallaltyiltiozatthieont,esotr Samples except that they contained HCl to prevent microbial growth.
000915
ire
Atthe thieniteinadlofDOCt.he T2h8u-sdaylostsesotfpseroiloudb,letThOeCy bsytiltlhesceonpthayisniecdalnemaordleysalwlas ncootmpoanemnatjso.r fTahcitosr,conattrolleasdtoesfno'rtnpornomveetabtohlaitzepdhys"iLcIaGlHTpWrAoTcEeRs"ses did mneottabroelmiovsem o"cLcIuGrHrTedWATiEnR"thmeesteabsotleirtielse cionnttrhoelst,estbutruntsh,e csaitnacbeolnioc* cfaotrambaotliiocn pofrodluecstsswaarteerussoulaulbllyemomraeterpioallasr ainsdusnmlailkleelry. because
TABLE 2
DOC Loss from Sterile Controls
Product
% aDtOCDaRyem2a8ining
FFCC--220063 FHCy-d3r0o1g7uinone
9914..32
8787..15
-
Adnayof0f-pwrhiiotre tporefciilptietraitneg ffoorrmeDdOCinantahleysihsy.droqTuhienopnreecsitpeirtialtee cwaosntrol on
scolnitgrhotllyabftreorwni2s1h,dayass.wasThitshessuoglguetsitosn thinatthheydhryodqruoignuoinneonies sntoetrislteable,
aOtECDleeasxtperiinmetnhte.preTsheinsceapopfareHngCtlpt,endtehnrcoyughoofuthydthreoqucionuornsee Otfo the 28-day
sipnoanptparnoeporuisaltye cfoonrtmroilnsocloumbploeundhumfuosr-ltihkeeOmEaCtDertieaslt.makIetss pitaratnial
parpepcrioppirtiaattieonnesswitohf
HgClp using
atlheso HcgaCslt,s psroemseerdvoautbitosn
apbroouctedutrhee
in
the
OECD
method.
CONCLUSION Tphreodupcrtess,entFC-s2t0u3d,y cFoCn-c20l6u,sivaenldyFdCe-3m0o1n7s,traatrees ntehaartly"cLoIGmHpTletWAeTlEyR"degraded in 21 days under the conditions of the modified OECD Screening Test.
REFERENCES (1) oTersgtainnigzatPiroongrafmorEExcpoerntomiGcroCuop-oonperDaetgiroandatanidon/DAecvceulmouplmaetnitonC,hemDiecc.a,ls
A1n97a9l,ysTiesst(LGeuveildelIi)ne(H.forG. thNeoslMeord)ifiReedviOsEiCoDn oSfcreSeenpit.ng 1T9,est19w79i.th DOC (2) Dohrman DC-52A Operating Manual, 4th Ed., 1978, p. 3-1. ~
Appendices: 21 -- DOaEtCaD STehsetetsGuidelines (Sept. 19, 1979) 3 - Graphs
* Catabolism is biologically facilitated breakdown to less complex molecules.
ocos1s
)
APPENDIX 1
or
Tokyo, Decemoer 1st, 1979
118/79/Int. OECD - Chemicals Testing Programme Expert Group C, Degradation/Accumulation
Mbdified OECD Screening Test with DOC Analysis
000917
@
Test Guideline C 118/79/Int. Test Guideline for the Modifid OECD Screening Test with DOC Analysis Date of last revision: Sept. 19,1979 Levl I test for ready biodegradability
1. Prerequisites
It has
Soluble
toin
betheknocwonn
whether
centrati
o
nther
a
ntgeset
emmaptleoryieadl
inis
the
test (corresponding to 5 - 40 mg DOC/1).
2. Guidance Information Kpnroowploerdtgiecsofofthtehebatcetsetrimaaltertioaxliciitsymoort uinnehqibuiitvoorcyally Tceoqnudiurcctdiobnutofcothnestitteustt.es useful information for the
3. Qualifying Statements Tuhletimmeattheodbioidsesguriatdeadbilfiotrythoef wmeaatseurresmoelnubtleo,f nthoen-aveorloabtiicle oervgaalnuiactiocnompoofunmdisx.turIets.is unsuited for the biodegradability
000918
Ph Is. 2 Stoa/s
Sept. 19. 1979
Modified OECD Screening Test with DOC Analysis Preamble
It has to be realized that the following procedure for the modified OECD Screening Test has to be regarded in some points as provisional since several important features such as the calibration compound and the new test duration of 28 days are completely untried yet. However, since the test constitutes a modification the official, widely practised and well accepted OECD Screening Test for the biodegradability evaluation of surfactants there should exist a good prospect for success.
000919
@
Septemoer 19., 1979
OECD Chemicals Testing Programme
Test Guideline for the Modified
OECD Screening Test with DOC Analysis
0. The test procedure constitutes a modification of the OECD Screening Test (OECD Environment Directorate, Proposed Method for the Determination of the Biodegradability of Surfactants Used in Synthetic Deter=
gents, Paris 1976, and council directive of Nov. 22,
1973, on the approximation of the laws of the member
states relating to methods of testing the biodegrada-
bility of anionic surfactagts (73/40S/SEC), Offigial
Journal of the European Communities No. 4 347/53 of Dec. 17, 1973) for the application of the dissolved
organic carbon (DOC Janalysis.
1. Methed
1.1 Introduction: Purpose, scope, relevance, and apolica-
tion of test and explanation of limits.
The purpose of the method is the measurement of the ultimate biodegradability of water soluble, non = volatile organic compounds in an aerobic, aqueous medium at a starting test concentration corresponding
to 5 = 40mg DOC/1 ( In order to avoid inhibitory
effects it is in the investigator's own interest
-
-2-
000920 .
-2-
to choose as low a starting concentration as his
analytical capability permits )
1.2 Definitions and units 1.2.1 Definition of biodegradability
oz= [|Do7 1. ae zoo] = Cc,t =- Ci 1 100
where
Dy = degradation in.percent DOC-removal at time t
Co = starting DOC concentration of the
culture medium (mg DOC/1)
Cy = DOC. concentration of the culture medium at time t (mg DOC/1)
Cyy= starting DOC concentration of the
blank (mg DOC/1)
_
Cpa DOC concentration of the blank at
time t (mg DOC/1)
1.2.2 Units
The degradation is stated as the percentage
h
DOC-removal within 28 days with respect to
the test material ( DOC-removal )
3
000921
@
rr
1.3 Reference compounds
1.3.1
Calibration compound
The calibration compound used in this test is hydroquinone at a concentration corresponding
to 20 mg DOC/1. Hydroquinone has to exhibit a DOC-removal of 260 % within 28 days, otherwise the test is regarded as invalid. =
1.4 The principle of the method
A predetermined amount of the compound is dissol-
ved in an inorganic medium (mineral nutrient solu-
tion, fortified with a trace element and essential
vitamin solution), providing a concentration corres
ponding to 5 - 40 mg DOC/1. The solution is inocu-
lated with a small number of microorganisms from a
mixed population and aerated at 293 - 298 (20 - 25C)
in the dark or at least in diffuse light only. The degradation is followed by DOC analysis over a 28 day period. The procedure is checked by means of a
standard (hydroquinone).
A control with inoculation but without either test
-
material or standard is run parallel for the deter-
mination of DOC blanks.
1.5 Quality criteria
1.5.1
Reproducibility
The reproducibility
of
the
method
is
appropriate
-
for a screening test which has solely an accep-
tance but no rejective function.
"he
000922
@7
I.
1.5.2
Sensitivity
The sensitivity of the method is largely deter=
mined by the sensitivity limit of the organic carbon analysis which is 0.5 mg C/1 at the present
state of the art.
-
1.5.3 Specificity, applicability
Applicable for the biodegradability evaluation
of water soluble, non - volatile organic compounds.
1.5.4
Possibility of standardization The test version with specific analyses for
anionic and nonionic surfactants is standardized
as "OECD Screening Test".
1.5.5
Possibility of automation
Parts of the test, e.g., the analysis,can be
automated, although hardly the total procedure.
The procedure is, though, well suited for being
operated with whole series of testmaterials.
1.5.6 Costs ( in 1978 Swiss Francs )
1.5.6.1 Equipment
Glassware
2000,-
shaking machine
6000 = 20000,- _
Carbon analyzer
Miscellaneous (pH meter, balance, provision for air donditioning)
42000~,
5000,=
ts
000923
@r
5-
1.5.6.2 Person - hours
10
1.5.6.3 A(pipnrvoexsitmmaetnets taostaslumceodsttpoebretat 750,= = 2000,amortized)
1.6 Description of the method
-
1.6.1 Reagents and materials
1.6.1.1 Deionized water
Deionized or distilled water free of
substances (copper in particular), for
use as a solvent. Water which has been
zed by distillation or ion exchange is
tcxic
general
ceioni-
suitable.
A high purity of this test water is necessary
in view of the DOC analyses in the concentra-
tion range of O - 40 mg/l. The contaminations result from inherent impurities but also from
the ion exchange resins and microbial devel-
opments (bacteria, algae under the influence
of light etc). Only one water charge must be
used for one test series which is to be controlled
beforehand by DOC analysis. If necessary,
suitable water may be gained by UV irradiation
or other means.
1.6.1.2 Nutrient solution Mix 1 ml each of the following solutions (a)
to (f) and make up to a volume of 1 1 with
water 1.6.1.1
-6-
000924
"gn
(a) &H PO, ALR.
8.58
K HPO,
AR.
21.758
Na HPO, "2 HO AR. 33.4 g
NH C1
AR.
2040 B
~
in 1000 ml of water 1.6.1.1 the pH value should be 7.2
(bv) 22.5 g of MgSO, . 7 H0 A.R. dissolved in 1000 ml
of water (3.3.1)
(e) 27.5 g of cacl, A.R. dissolved in 1000 ml of water (3.3.1)
(4) 0.25 g of FeCl, . 6H0 A.R. dissolved in 1000 ml
of water (3.3.1)
`This solution is prepared freshly immediately
before use. (e) Trace element solution
MnSO, . 4 H0 39.9 mg (30.23 mg MSO, . H,0)
H3BO05
57,2 mg
SO, . 7 H0 42.8 mg
(NH)gMo,0py 38.7 mg (36.85 mg (NH,)gMo,40H,0y)
Fe = chelate (FeCl5,EDTA)
100 mg
water 1.6.1.1
1000 m1
-
Sterilisation of the trace element stock solution
at 393 (X) (120C), 2 atm., 20 min.
ST
000925
n
7-
(f) Vitamin solution
BNiicoottiinnic acid
0.2 mg 2.0 mg
Thiamine
1.0 mg
p-Aninobenzoic acid 1.0 mg
Pantothenic acid
1.0m
PCyyraindoocxoabmailnaenine
5.0 mg 2.0 mg
Folic acid
5.0 ng
water 1.6.1.1
100 ml
The solution is filtered sterile (0.2 um). Instead
of solution 1.6.1.2 (f) 15 mg of yeast extraxt
nay be used per 200 ml of water 1.6.1.1.
1.6.1.3 Biodegradability standard Hydroquinone 99,5 % DAB Erg. B. 6
1.6.1.4 Hercuric chloride solution 1 per cent of HgCl, in water
1.6.1.5 Shaking machine accomodating 2 ltr. Erlen-
-
meyer flasks either with automatic tempera-
ture control or used in a courant temperature
room at 293 - 298(K) (20 - 25C)
1.6.1.6 Narrow neck - 2 ltr. Erlenmeyer flasks. (Creased fluted. . fflualslkys calreeanerdecowmitmhe,ndeed.)g.,Thealcfolhaoslkisc muhsytdrobcehlcoarriec-
Jr
000928
"gm
e use, rinsed and dried in order to avoid nation with residues from previous tests. .lasks also have to be cleaned before their cst use since they may be contaminated.
1.6.1.7 Membrane filtration apparatus
1.6.1.8 Menbrane filters 0,2 um
-
1.6.1.9 Carbon analyzer
1.6.2 iInctcuhlaoetfi:onthe following three alternatives may be used 1s inoeulum or a composite sample thereof.
1.6.2.1
Ino:ulup from secondary effluent The inoculum is gained preferentially from a secondary effluent of good quality collcted from a treatment plant dealing with a predominantly dorestic sewage. The effluent must be Kept under aerobic conditions in the period between sampling afd use. To prepare the inoculum the sample is filtered through a coarse filter, the first 200 ml
eing discarded. The filtrate is kept aerobic until
sed. The inoculum must be used on the day of collec
ion.
1.6.2.2 [inoculum from soil
100 g of soil (fertile, not sterile) are suspended
lin 1000 ml of chlorine-free drinking water (soils
oo
With an extremely large content of clay, sand or
rganic carbon are unsuited). After stirring the
uspensicn is allowed to settle for 30 minutes.
-9-
000927
@
-9-
The supernatant is filtered through a coarse
filter paper, the first 200 ml being discarded.
The filtrate is aerated immediately and until use.
The inoculum must be used on the day of collection.
1.6.2.3
1.6.2.4
Inoculum from a surface water
An inoculum is drawn from a suitable surface water.
The sample is filtered through a coarse paper,
the first 200 ml being discarded. The filtrate is
kept aerobic until used. The inoculum must be used
on the day of collection.
Composite inoculum
Equal volumes of the 3 inoculum samples are united,
mixed well, and the final inoculum drawn from this
nixture.
Tr
The suitability of the inoculum is checked by means
of the standard hydroquinone.
1.6.3 Conduction of the test
1.6.3.1 Procedure
The test materials are evaluated simultaneously in duplicates together with the standard (1.6.1.3) and a control test with inoculation but without either
test or standard material for the determination of
DOC blanks.
-
The standard material has to attain 60 % DOC re-
moval within 28 days at a starting concentration
corresponding to 20 mg DOC/1. If (60 % DOC removal
are achieved the whole series has to be discarded +)
+)Tthhies1l9imdiayt1vserbsaisoendoofn tphreesteenstt.exAperreiveinsicoenwiotfh - ttohisbe lcinointsidorereedvenaftoefr thtehe satcacnudamrudlatmiiognhtofhaveexperien-
ce with the new 28 day version of the test.
-10-
000928
@n
-10 -
A stock solution of the test material in water
(1.6.1.1) is prepared. So much of this stock
solution is added to the nutrient solution
(1.6.1.2)
mg DOC/1
isthaatttaainceadr.boTnheconscteanrttriantgiocnonocfent5ra-t4i0on
of the standard hydroquinone is, though, 20 mg
Doc/1.
Two reaction vessels (1.6.1.5) are each filled
with 900 ml of the nutrient solution and inocula-
ted with 0,5 ml/1 of the inoculum (1.6.2). The
opening of the vessel is covered with, e.g., alumi-
num foil in such a way that the exchange of dir
between the flask and the surrounding atmosphere
is not unduly impeded (Cotton wool is unsuited
because of the DOC analysis). The vessels are
then inserted in the shaking machine. The tempe-
rature of 293 -298K ( 20 -25%C) must be maintained
unchanged during the test, and the vessels should
be shielded from light. The air should be free of
pollutants and toxic materials (chlorinated sol-
-
vents etc).
In the course of the biodegradation test the DOC
concentrations are determined in duplicate (1.6.4.2)
at the beginning (day 0), and on the 27. and 28.
day. Three additional analyses have ta be performed
-
a1 0C09r9
Pn
-11in rather regular time intervals (~7., ~14., and ~21. day)
The analyses are registered in the attached form sheet and evaluated.
Only the necessary volumes of
drawn for each determination;
culture medium may
however, they have
be
to
be large enough for the membrane filtration or centri-
fugation preceeding the carbon determination. The latter
requires differing volumes for the different instruments.
Evaporation losses of the culture medium are to bemade
up by adding water (1.6.1.1) in the required amounts.
The culture medium is to be mixed well beforewithdrawing
a sample. Material adhering to the wall of the vessel has
to be dissolved or suspended before sampling. The membrane
filtration or centrifugation has tobe done immediately. The filtered or centrifuged samples. have to be analyzed
on the same day, otherwise they must be preserved with
0,05 ml of theHelly solution (1.6.1.4) for each 10ml
of nutrient medium or by storing them at 2 - 4C, The
biodegradability test is valid provided the standard
-
exhibits a degradation rate within the specified range.
The test can be finished before the 28. day if complete
mineralization is accomplished.
All steps require great care and cleanliness of the vessels,
pipettes etc. but not sterility.
-12-
000920
Dn
-121.6.3.2 Calculation of results
Tfhreomdetgheraddaettieornminaattitohnes tiomfethteisDOCcalccounlcaetnetdrations at the beginning (C,) and the time t (c,) according to
Dy T|| 1- -- [ " | C1t [x 100
|
Co = C1 |
where
D, Cc,
= =
dmeeagsruardeadtiosntaritninpgerDOcCentcoantcetnitmreati on
of
C,
=
the DOC
inoculated culture medium (mg DOC/1) concentration of the culture medium
at time t (mg DOC/1)
Cp = starting DOC blank of the mineral nutrient
solution with inoculation but without test
_
material (mg DOC/1)
Cy) = DOC blank of the mineral nutrient solution * with inoculation but without test material at the time t (mg DOC/1)
The
0,1
d%.egrTahdeatmieoannsraotfestheareD, cavlacluuelsateadretocaltcheulanteeadresatrd
-
reported to the nearest full per cent. Results
-13 -
000931
Dt
-13 =
ending in 0,5 are rounded up to the nearest whole number. The course of the degradation test is followed graphically in a diagram as shown in the attached example. The results are reported on the attached data sheet.
The results of the degradation test are valid if the condition is met that in the same test series the standard yields = 60 % DOC-removal.'
1.6.4 Analytical means
1.6.4.1 Membrane filter0:2 um, 25 mm (1.6.1.6). Pre-
paration of the filters: membrane filters are
impregaated with surfactants for hydrophilizaticn.
Thus each filter contains up to several mg of
soluble carbon which would interfere in the biode-
gradability determinations. Therefore the filters
are purified from surfactants and other soluble
organic interferences by boiling them 3 times 1 hr
each in deionized water. These filters may be stored
in water (1.6.1.1) for at least one week.
Other membrane filters are suitable if it is assured
that they neither release carbon nor adsorb the
_
compourd in the filtration step.
If the samples are centrifuged, this has to be done at 40 000 m sec"? ( 4000 g) for 15 minutes, preferably
in a refrigerated centrifuge, in any case (40C.
(Remark: the differentiation TOC:DOC by centrifuga- _ tion at very low concentrations does not seem to work well since either not all bacteria are removed or carbon as part of the bacterial plasma is redissolved. At higher test concentrations ( 10 mg C/1) and the same
small inoculation the centrifugation error seems to be
comparatively small ).
000932
Fora Sheet for the Nodified OECD Screaming Test
on
eootretisceaalneatreestsacvoenrei.a:ts
tSnaorcbuetnuna:nalyser:
wen BEST COPY AVAILABLE
A:
am1r 1 Controls: So0to8e8asta hSieeion en senate
Toe 1nentpc"
5: Carbon doterainations:
TT TB SaaS TE
ineral mueeient
fest ater
| |!
[~T 1TT 1T T 17
or
CLT
rfoilotneoereismitnmnemsietarisaircenuentiseun
tae
~ T 1TT 11 w [1TTT TT]
= ee | /
_
Co
Boslustion of raw sata:
Tio
LL
boc - concentrations
a
ber v e |]
2
wn
tte.
o-dp2-
Clem
i Td 093%
@r
.
-au-
1.6.4.2 The DOC measurement
The sample withdrawn frcm the culture medium (about 30 ml) is centrifuged or membrane filtered immediately in the filtration apparatus (1.6.1.6) using the membrane filters prepared acc. to 1.6.4.1. The first 20 ml of the filtrate are discarded: The DOC concentration is determined twice in the remaining filtrate (about 10 ml) by means of the
TOC/DOC instrument (1.6.1.8). If the filtrate
cannot be analyzed on the same day it has to be conserved acc. to 1.6.3.1. The DOC measurements (mgC/1) obtained are registered on the `attached data sheet and the DOC concentrations of the culture medium and of the blanks calculated for each sampling time.
000934
#
-
*
APPENDIX 2
TT
-
.
resi3n1s EonrviraonmeRnt1al68LaboSrEEaDtorSyirDeastnaingSheTeetste
'
Les neguest wo. 5501S
ATDnahatateoyrasonttf:icySatsldarst 5o.4 fTesT- ter :tSonal1 } TE ae r1To" r G0 a SEStandard Material :_hydrosuinone, 98.5%, Lot100147, Aldrich
Te Teneasguutroand:T ,TTaoolfaTnaedstaamtoarrir SUT Seone S127 5at Capefrma male]sunlcos] wewsetstaemneatetrerccoSrsesttmosonntt lanplant
A. Determination of Toc! of Test Material:
Ze BEST COPY AVAILABLE AIstoacktonstozleudtiaonteofrthe test material was prepared by diluting 117s0g to a final volume of 500 al
TSktoahh rmotuTtio]n [stock5TsEtation| test=mahteerial | a ihlms oroewe sfulSntevkwe easmuastt eadrlsyo cialcpulratoe stphceeorraircuaresssary
8. Carbon Determinations;
-
MWiinneerraallnmuuttreient] npereatyatn
TE dvelT er oo[i fee hSoatteirest n1a/ta_| |tn Fertesemaarmintar Cle) TW A
282
40.0 E PTOFaod 2r 02e er e0i T 2 id
P =e r
[22 |wo HE S EESE aEe rESR eEe g EE IeS TT"
Hi`Mnianerraall nnuutrrrileonnt]
sesae tmeaterTel|
hae
C. Evaluation of Row outa;
lett eR tnLaefJIT] 0.
Crore) |-02|04 |12 [0.5] < 00
TBA Tei ts
[veoTr TAbloT! o [or[r2 efoc [oor Come |14 |-00 | 04 [12 [06] 6
CTes nalma|fBaes| mummers Frese
redatio=n %30 gemovil sf5erdogs Tncbetion Tn EAARL.
Na are vied 1 ha coralevtons:
[==[rzfoc[es Tar[as] reve* mt
1. 2
The 0c
test material 1s completely analyses parforned more than
stowliucbeleonIntwheatesre.m
Tsahmeprleefovreer,e
trhuenTfoor =ue0lGi.ty
assurance
PUrPEseR) 00935
erento orTE SA eraantngTore BEST COPY AVAILABLE
LGaabca'Roefquessttarhto,;_S541 TTToheenasrraeatoiiT eTnock eo RTor T itAam rteisas In rAiBcorvaa3etle sn/n TOTSEtSandard Material: hydroquinone, 98.5%, Lot 1001, Atdrich Trocuton: 201) and ponchToed SecaTry S10loans fron
A. Determination of Toc' of Test Material:
wA hstodcaktonstozleudtiwonatoefrthe test material was prepared by diluting L=OG=O mg to a final volume of = 500 ml
Te mn'
stockpssotution
vo walks
TaThotiosounmtresoouffltttoewsetsosrmtautsaeorduig:l0 c5o0lcpurleaptaeret8hecunreecuersesary
2,120
Tye
5. Carbon Determinations:
-
Yori ne thems
ERElEEAEEA E net amtsna
STahartetraian mfaat|iSnF Pehnatsemacamrgtin Oleg) TW AE
Co7 [ICezlTyCo[C03 3Pl[379J C[S30
q2e
Jo.0
r Tr t
E Sd Tee er eTTe e iaTie
426
Yoo B -- --r 1 o r e r
TEle ee fee) SMionteurtatlonnuuttrdlent|
EPH Camm Thiele =
or | Foe Torti oe or Cc. Sentai of toc ce;
rT
SdeeTr en[o7syTeod| E0n
eTvrr To i oat tr ea ob T=
Come"
=o
77 oe| @
Tosfoeafowlo9n7il| mamas ton, [or
eagrerdeeriionn=X% p00% ggraomvae affiter xodogm eneebattion| gespd sim -
aT vied 1n th cateutations:
[T Jo[wa os|s 72 | awe t
1. 2.
The 00%
test material Is completely analyses performedmmoorreththann
stoeilcautbwicleoeonIntwheatesre.msTseahreprleefowraer,e
trhuenTfOoCr
=aqDluaOliiCt.yty
assurance
rrpurposes.OTE
)
er4 aoErnvnironmenSttalHAASLEaboraTtorey rasnOsitna gSheTeotre- BEST COPY AVAILABLE
LRSooaobtloyRaootf:g.uSetvsaarKdsoa--.Af.;S_TSe5Tsr4ch1IolT 5TES. Tost Katara AERP, FETT Tor 130:
Standard torte): hydroquinone, 98.5%, Lot 1001, Migrich
THTharasaoeureraentdi:cTaoc]sJolo,tofofTsnedsTtesnthaottaentreircetla:elSsEedHCO[A1T2.CU0eadsryA00AL TESaHD)Tuant ESVeena mun anteater sraatent olan
aToma Gri apanicles]wasiesater trastslant ------
A. Determination of Toc' of Test Material:
Awitshtocdkatonsiozleudtiownatoefrthe test material as prapared by diluting L2YS pg to a final volumm of 2S00._a1 TTr oShooTcnk smatuatioTn ||stocklTstCotucion| tes`twamlaktesrial | Taahmtiosuonmtreoosfufltbeoawtesasnmtavtieeorduigtto ciaolcpurleapteereth8ecnueltcuersesary
8. Carbon Deterninations:
WSionuetriae)nniutornient tnesatamanterial
Inoculum
Cm lero oe ToToarntaeee,riaolf tmIa/entnt||C[ioonftcsfueatsstaet;meadtexrmaital OSOC T -- cone. steerWAx days AIncbation, wal]
To [e grylit aTlaayer 1T3a7e Teer[7713
20%
4o.0 ro e PF s te pea
ee fre------------------
BHoE sTye Sgr tgrr a
208
4.0 Srr -- -- -- ---- --r -- ---- --
MSionteurtailonnuwtirtlhoonutt| st material
Le 75 e CEreCTshCs TS TeYnB bey
He
b
=
. Evaluation of Res Date;
orm Fle Levanfoc] 1 FT re a Toe ret Ts reTe e oto
4
B10'
Cllaye = | 14 |=0u| of [12 [ob|
[Fr] epGeorredoarimona= % DUC ggarvetl oagfer x days TheWetIon||seeu aseched d sm ection S, or
[0 [oon [seofoes ona] wea | RVmeasmluuslestesvsoefrmebilvaioendkmnIon.1thaesndcianloc.oul2antwieor,ne,
[= To {a9 Jae[ou [or |] -
ave t 7
1. 2.
The 00c
test material Is cooplatly analy"ses performed more than
stowliucbeleonIntwhaetesra.m
sTahreprleefowreer,e
trhuenTfOoCr
=qu0aOlC.ity
assurance
purpos0as.00927
|
reso mnteveretvetst. BEST COPY AVAILABLE
Ler LOaabteReoqfueSsttartNo.o:_Z fTe5s- 5t4:1T S I=B0
TA e eT----EAETAStandAardbaE terial: y uinons, 98.5%,
Lot 100147, Alrdriech
A. acs Determination of
!
vrof
meee us Tes
ortal;
pero
1
3 man. 1 v0 rot
=X p5o Tore | TEn tHe dRp ST er
LTT] evauamion or cAristanoy
on sums
CMAOTMEPROIUALND--ABSOEEVE.STANDARD
|a
en 0] e Ts a rg
Hainnedrwaitenynrlont
wet wee]
30.6 | 200
EERE [eo T7saT1zi3r[T27e0 TT3a1 T7 re317ib
ee----r----
|| 3e 06 |201 FHE IPnAsEE fraeo[r 2]7E 0r0e 6 [14
|
----
mo]ix[[ob/[18d Joe
Wk [Led eRE e e
Tnoculum.
1
Terrier Llp pnb
aeba 5.
IF To
fetefer fo foe] 0.
C. EvaluoafftoeiDoatna:
Cotnve= tc [1g |on | 04 [12 [0b] 0
[Flom ooprpions =gieoth fir Sn | gnssid ssi LEO0
[0[ees Jos[906[337] 972 | p tmsoeimon vom ton [+ [907Jaro le2c|laoegs| 0a" T2 oa Glin [=[7 92[97[94 [93 | 1+ omer 1m ce opay sto a wr e aretorv es 1r0 w ric rg ee
ee
Eee
puter iby
EEE
Ee----= lhumdm = St
Sem
= = =a i
=
Date of Start of Test: h=11-1980
ed
ewer Aver pecan wee eo]
[ELAR
eee i ee ee
--=
= = = = >-- + -- |} i = e
e= t e i
e Po" f rf
ne ee
re
eee
pe--r= e eeee n ep ee mn
2
"8
USER GF DAYS. INCUBATED ----
4 05h 4
TT
P=
es
Cee
_--e == -- ee
--
me vnc ss mts So T NAE RGp R Ee ERCaDinMosdiafideed Shceresenoilng] Test with DOC " Analysis
Sm Er re ens H E E Fee E mm e mE ee
AELoy E -23 10, ee S eE eE ]
--------=- =
Ee
= f=--
=
= = =F = == 50
=
Ee
Ee
ie
eet
= E = t = Ee
SEE Ea EELe E
E 2310x EES e e e e e e e:nea ahes eeeeee ee eTee a eE] ) Eoo
w3r
. immer
vrge
ee
E ----e --------
Tom
--------ee
m an ova tcoei nal Lnoboratery
-llfr
ps
Lab Request hou: 5541'S
a
festMeterlal ANTWERP Pooler botoales Coo oo |
w = = ---------- see ZW80x) = =" rr t " --t Ee=
Ei g E= e a
-- == ee 30%
~
wf
ey
lm aE
;
--
So
q Sf eeeu eee e e ee e pr
ee
2
NUKSER
OF
DAYS
.
INCUBATED
I
od
zd
-
SE EE ee
H nanaE n LavonIs
SE SEoTE mI a
ee TE
Dwire re ert Cthhoanorunsienesomfouls
E= Ee Er E
=
w ok = -- ee te --
0 er a
=r wg 701L 8
--_--
a
=
SEE
= whi
rr
ST, FREER
|
2
wie ee ee =
w-- e Ef e EEe e e r e e e e e e e f F ee efee m es - e
g c
pr --
: oipre.
SM
LeA SSHLS
septs 19: 1979
:
260 Crentente Teasing Fromme
1\
*;
TeTest ne Ouideline tfoerstthweicMhod0i0f0ierdales
he
tote Bierman Directetsie,
iv suns. atrstive of for. 22
|
states
relatiayngaftoor mnientehoadSpsopanoimfcaatnteiisoitneinogYfot.Shhee4
biodegrada-
3di/s5so)lvoefd
\\
Saoe ssreon 0S eis:
|
1. Method
ee
:
\etatile
organic
compounds
in
an
aerobic, aqueces
corresponding
;
\i
edium
een
aito magstDaorGt/i1ng(
tIenstorcdoenrcetnotraavtoiiodn inhibitory
!
bi| i
iH
.
1h
a | :
Coe
.
Wi
000943
renditiono t n ei. BEST COPY AVAILABLE
LDabta'RoafgueSsttartHauo;f TSe56r1 SAIAG
:
AT oalyate : Ved r A, Sr ohe es nee
Stondard Matarlat;_byiroguinens, 30.35, Lot 1001, Aldrich
CTTroaoienremam irgsooaietSFsStnToohviinguaensnoettbCgeiAno41ntTeodr(a5TThG1croCgRnUTadULEenT leCarboEnRnt&),romS a my glcios gase so
u
A.
Determination of voc! of
A stock solution of the
Test
test
Material;
mterlal was
prepared
by
dlluting
1170
mg to a final velume of SOO
a
ith datonized water
EerTtaEsotot]ion' |atach=TsEoluTtion| castTamEiatseCria)l | s Ts ru oofheSan eaatatamat aettadrlig, ctocopeprreceepeareere044 crulcutlieuree n
5. carbonoaterainasions:
:
MSTioensteturcatmloanntlueterrhient aTnoucirtim
Coma m LPao h[dE a[e0 . MSionteurnatlonnvutirsleonnt
= TTheer ost materiel
STaortetryial mgt/al 282
282
[ionf Falsatsk,matem/lrial Pa
CT TW AE [ezgBl al [aerZ[oorl
12
40.0
eNP n oe e Tgar ess TSri eeae r]1
I
-- 7--
CTI PA PT Pe
40.0
T r Fa e r ] e
Tbe pH reha treeeri tTooyrtr taerr t.o
5) 3,
-
-
TT 2
Tor L orofor [ac] C. Evalustioofn Rae Data;
Cope= 14 [-00 [0d 112 0.6]
et RR D0[es[srefms[1a3a0]| mTTaasra voied nIn thecmaliculations.
[=[= [es[esfar[0s | ave" 2
1. 2.
The 00
stnessltysmeasteprairaflor1ssadcommoprleetetlheyn
stoullucbeleonInthweatseru.m
eThleresfowrasr,e trhuenTfooCr=qu0a6l.ity assurance purposes.
000944
E FEE o l T h EeciEe] nRt wEenT n em [ ComE e ERAe T
fEToiE)E T Ermo a d
CoS receeoMmee seas ee DS
_---- eee
er|
of
= = EE e e e e ee e e e ete e e e
ee
|
se Ea em e
rTes = eriTf
. = eR
rr =
ie
P W er E E ee =S eeee E= ste
mp Ee ee ee ee f e
we fem
fr
fa 2
E EEEEEERTE LE Eee S eeBER
leEig
Sg
T | h en ws reo ey ash8
renneoutr vt BEST COPY AVAILABLE
SLoaba'RoofguaSsttarsHo,o;f VeSrHeI S --EIED
ATTnhoaenloyrnseaeti:icaladie EA A,oF sTon eFssarr se f: (ACTALCULATED)Standard Fatorial:_bpdroguinces, 38.55, (ot 007, Aldrich
oTTarvoaraeuurnriendT:.Teoc]esolrofaSnTedsTtpoNmcahltsaelrYielH: VredoraSesSa=rngdeanrtySCIaCrbonxuans featsfats sun
"
A. Daterminstion of 30" of Test Neterial
iAisntocaktomsiozleudtiwonatoefrthe test materiel was prepared by diluting fL24y5 mg to a final velume of = $20) oonmon'setution |stock=lution|tepstTmraEt)eTri]al| Ta monunrotofnF teset nmeaterieglettdetcoaplrelpeasreusncualctuernery
b. coarabtoen rninations:
.
Yi"rarat mutrlone TpnodnremearerTat
Cf omm lee le oTheakhy aIa/at
|oiFn fSalnatnmatereinal||anyaeise [0 Tf WB TI 72E378ES2 5
TB 0 PC
208
4o.0
pCo048pTeIS PS 5r 300 e2 7 Err
-- Tr
C TTE TTaal
208
4o.0 mtger-- E Be r -- r a -- r s -- (r -- --
H--EMSionreurtailonniutsclneontt|
C. Evaluation of Ree Duta;
-
Fe77r. re3 r hCaTSsten Of
TE TEons [oe] TT At re e Tie eToe rre an
Te1r [lem fou [e on foele Curae [19 or od [12 [os] e
erp [mr |eapS
EO] gt sR
Tr hea 1 te cateutavions. baw t
=LorTosToe[oe [oe 1.
2.
The 00
atneasltysneastaprtearlforImsedcasmoprleottahleyn
stowliucbeleoninthweatsera.m
sTuhraprlsefovresr,e
trhuen
TfooCr
=quOaCl.ity
assurance
purposes.
000946
[ oE T I p y OECDHr odif fedScreeningTestwith 00C Analysis
Ce Raname
dha 5
E EEE=stEr
3
g
|
E Tr eee esGvew ri Aevr pecs worazes Ee
T==8 Ere
--e eee
Ee
Ft meome =
w - ee Smee ee = pe
--
NEE Ee Le
ron pee
ES = 1-51" Peolg Ee Bo ol /
| mii fl
ee ee
fp
r ET eTT ET
Sf
i
Somme =F BAL ==
Sr
frny
Ee
pi
So
Ba
EEE ee ee
o
EE
nt sm ois
sree
|
C4
.
Lab Request No.:_
S41
rm ees Te resningTest-
EEE
A. Determination of )
83 rial;
BEST COPY AVAILABLE
8. Carbon Determinasjong:
eT mer [e me |ame l[m] e | TT anit Mineral nutrient ocr
42e | 00
ee [o[ e 92[ 3 <31 123 6 [0 39 13.0 ft --t--t+--T1T--T
r r elt [ae e[or]1 EE TT 426
40,0
a=
HE pellealeoc]e [Ere
1Te
Ereressiere 1
[or[oz1751 7Lor
1Heer rebra
ny
of |
FE | ma ET
ft SAG
[0 [ees[se[d 930l ] 9a0dm| mses wis,
[= [os[io [#372]|
000948
=e 7 + +1 i SESE Pome ITE
TT ee
a Enirencental Laboratory
ea
o
&
|
Foss
h OaEtCDeHrosdtiFalrae tdoSfcTreeesntisnbg eTelsltweisth006 Analysts
-- ES ei-- t j-- a=S -- D
mmm
LabeRee wnHou wsSnSseMSmeese ots
Hmmm
Coo
Pa
ler wpa m md Sooo
eee ee
of S ==EE
------
!
Em
|
ee
=r
E eey ee eeeeeeee e ee
PEE
rr
ee ee
Er
Mis
Am
MoErr f E emeree
FE
==
Er
fe
le
Re o
men Camm
Co eee
ee Eee Ee
|.
os WISER GF OAYS J CURATED
5WP
re3n1 netrB onmentalE LaboratorTy eoutagseTeene BEST COPY AVAILABLE
TLSabeoheaaguaTsat rno.TeSnAIS ER ET --
Standard rariar:bydronu
Joop, avgcten
TTSThereveovneroeerrtaeidS ncnT)oTS36roSroFTteteo secaFreo trorneEtE T REERAARLSA hT=e Aens, caormE sa pa senclst eis R m0haaasAtt --
A. DAetaetroimcniknt_asotoinloeuntdioofvTnaotcofe' tohfe Tteesstt Mmaatteerriieall;was praparad by diluting _ _mg to a final voliomf a1
[TT evacuamon or cAustinay er F otetin stccLkstetactan|toreicacerial | t mmo dsaermbeo arI)coprp5ceurriaeeCo
u. carbon peterminations;?
COMPOUN--D SEE STANDARD MATERIAL ABOVE .
Cone ot||caStemuTanied oeo |e oSoce~ coTe. afWiar x doy TIEnntize, 271Te
rerea)tFneurtien oodniamn ie
[ow P alesS [20 [e=oe17r3i17e7]1} 30.6 | 20.0 PyHfeeeb--------r----Sta----rH----
ro7sltoef]v {o8 Cr1e 2 e 2r 7 Aee 5
30.6 20.0 ATn t UTeera ger a t T i-- 1
wn
EAE
1
orm
erie
| Thealou
Loa
Jas
Lo
[ 1o 1TroeJe n or [rafoclieeate
. Emi ot ta
Comma =i0o =[19Jo|09|12[0b] o
PE rmETTTo) fet nimdasa.
[0 [sesloos soefoar]gra | I wier d 1a owscrieatoaeonns
[57 [referlou[43 | le "TT
1. 2.
0h6e atneasttyaseastarpiearlforIesescosmoprleetehleyn stowliucbeleonInthweatesrm.e
aThreprleefovreer,e trhunTfor=Oq0uCa.lity
assurance
PUTPESES:
000950
| E C mE mE m e e nCo ut e A Emm
a
re eet
| reer esse ss
Analyst: Wade A, Schell
DateofStoafr Testt: 4-11-1980 Lab Request No.: 5541 5.
Companion compounn)
EBESE pEnin h3ey
Ea
a Mme
2
a HEran
=a
(eee = = | = =r
= w fi= fe e ce= e = =
dae
==
We
ree EEE
r NE = nae : Es |hs of
df Tf
E EE e
e= e EE] GEE
4 : F Ee se msEs EEE eyoJ
NUFSER OF OATS INCUBATED
Da
$
--
' sean
.
Zed
roman
:
SAMPLE DESCRIPTION
BEST COPY AVAILABLE
A
tue vue,Lf5
ENVIRONMENTAL ENGR. LAB -- WORK SHEET
oun:
[rr
4-08-82nT --
tts Setting Alf
Lo ipsa X. anda ps fena Tern)
J a) Lordiedorie Nay HPD
2H, 5 = 360
pe atoll,
= 0.798
FW=14190
seveitup) pilot
(by (a Cla@Ha0 waa va Ew= 147.03
roveluhud = 1:32 ) Yad itract sume wind iota Jf pili 1:6 1.2 ()
ee
-- = 35 tyes pao -- - sillele site used fo
oly
.
-- oe ------i--
ot patios; and hl
snomoiddatrad
0.14 pf aprstetons nd
v
4 Drorluors Ege "4 yeiyed) Sige --deand), fl%me
--reus W id SY yrgedenClin, prod bupt gus
A_$0-50
>. 2
MoT Sd
2.4.
po jets pd He Aur (2) atsve Aouad
.
= EAR
ur
gayi
2-50
6.2.1
-_
kbe22
-Z
; pe
-
SY
~
Le - a
Jd ee a
v
lm
000952
LR*F SSH ~ 5 BEST COPY AVAILABLE, o,\, 2 ofS
sawrLe oEscaTION -- ou: 19-8l 0 ENVIRONMENTAL ENGR. LAB --WORK SHEET
Nota -- Mod). OECD <5 onemmiing aut,
"6. On dau-
14-80) orvnandineds Sodomy of Tiel
2on age1(4-10 8) frmoming Bid emsssshadbadiidef0--
.
ud 7
} 1-2
3- 2
D. ON pau 1o [(-21-80) Jamming Bak 2lwsaod online ly,
2ha dD v0 GU Foot ptodiss Bat B se val
~
ADE WAY..: O
Jo. 0
ain.
PG
dos -2 S-02.-80) frmening ee)Lak aslulims naticl
25.08 alr~ae pirsioted
4
vy Lz
TE Te
sahil ane poaetirls adel 4-3 pe
light
i ANrrrseach A yal
\ ares alipoand
Ales
unit
J ITE, Ax i
bagnrniah
Hol
[9 me 1ml o2, 523532) and 3-2
Gani
rm Prestewideii of rt
errrarSera %
PY----------------
T ----__ -- _Rmm --_--_--S-- $-- _----;-- -- sS ------ ,--
_--
FE--_--,--,----_,--$--ee--_--_--_--_r------------
ee
SI mT
]
000953
en
BEST COPY AVAILABLE
2 File: Papo, 2541 S
ENVIRONMENTAL ENGR. LAB --WORK SHEET
Jege 34h5
SAMPLE DESCRIPTION
one: 5-23-82 Anyi LSGhel)
Hes
eedoC Gey Te?
-
Teborate{catesspre) said ona Th Bathe litping contd etbs btoiad fr? sommee
Be Filtre words 020sm Mitead omitsfll,potas cE
Mo ohttis=Joss oddsai(BisGl bisnacytm) coma ssl--
to _svtract asd fo yo ison plac Ch+ Tha axTrae
umfe iend thoes hte PCy poagase ltr papa
1S
De
_Laklansa otra sven 6b i th
The dian mapinalivs en
danke.~~
Hhobth day stcsdedHe
Tor 80sscseans rte sls) ith hobing oloaniizasid slo
1s9eheerne)lina=ndarussaeadsocf eLrnaodd aadne t(6) Admanpe loder sila FDtaHiall
Td alld He stulee to atthe ged Rhos dicenlivis
he Clann rin Arant phage
el rt
7
rire ssid b $line
?_
20 ole prinh Dannl = od He srerinas dunt dae toy tian
tod. pn f
TE ML 000954
/
ooEsePY AVAILABLE
ENVIRONMENTAL ENGR. L--AWOBRK SHEET
ne E43
pra das
J sAwPLeOESCRIFTION
owe: C2372
Amst: C a24S. O
[rh----
pontonoed {pompx
S-----re--
i ered = Lroasa & Si
Ha protons madivars vide A lone
20* anl rlwil l ({4lahality: Dil t4h2d0 "1pl. t t0.0l --
and era's A dark os Fin ity DDNea lesA
veraek
25 nk of poste sans pthrors fn tech tmp g
iygruel oycrAavd,garuriing [Sod anda
Lin. baleen J
Arnd Pla Cont dons
Ahan. Aina
and
Air
Jor any
0 wb its Ho LL halen andl esCleiniy mde filfnaZ
- sn 2 7gona ria fl PE
.
3 Np
CL pdtenle, [don oY come. HC i added +40 do 0
A 7 Ltrs) oveni he
boii ide oft
a
of 1h oa
5
NY
ght <
He rinks were toped are frill loved re
ped raf Nigiralid] suihil Spoe-- analand on DOC:
2%) nd i Liniirts sanitd HEL 4p a orsnLovons ali
A
DO;C ton:lm se 7 prnlecal if wma = AavddV by7 Sor
pond
(
vould
amgols
tr publ pcos
fran' lili
deal,
Thia
is BT
boot on
ce%
Lava ls)
ol bate
Lapdog Lthalots
HL 700. pal oh) The
patutesn
plarde
waged 5
habit
CVU py fond vnirde Uf TonidD He Cl, wnant wed.
bts
(A) Lybr mgm 0e-S20
ateng ' Wand 4TME4, 1178p, 3-1
Rowe
.
000955
| TempenesTMe BEST COPY AVAILABLE
om S54 S
Fie: pete, 20S
`SAMPLEDESCRIPTION
]
ENVIRONMENTAL ENGR. LAB --WORK SHEET
oAHnuea:A 5-23-80S TT Veto - Yok." dE Sons "ast Fiz
pindnedy Te arouse TIE orbital,atich of a
Atay
orn dD
trdein
Fiiaaitlh).to
The
He
tirnihl
colinl =
p
vitailer, Simplo sdine wrciholnawe) fon
pe ofHe inhsnideile wrne vrtacwslfor
He fag =0 wed day: 2% foc ller.
eS
TT
} RPaenedllea S}iSSTtewlroirolte fp[4eTAI rdslee o:
SS
| DOC = mg [A
0, DOC. Ts pramengofbnatiag _
DAY-0 | DAY-2%
_
(50k 53
EE
2 (zen Pian | pn ase = 3th
2-3 (Fe-30n,
3-3 (rea
Yodtu,| Gms gear: 387
spay| Ghd vp (Cleiliies quad.
3 Cidosmnne ) E3015 FE Geld ne,
) "00C Romansgs DLot yp ef leodd Jago? 09
DOC hey 0 --DOC 2 A day 0)
J--
mu seerme BEST COPY AVAILABLE
re SB SUIS
SAMPLE DESCRIPTION
ENVIRONMENTAL ENGR. L-- A WOB RK SHEET
Pa al
o one: e 4-- 0B-8O
HooT
Baa poaTamn Tord Srctisrg
Tecone: S
Ad{ ak dotwt oDh oo dAt ltl
1.008 pr Hat Ha a Toe toncorntration
a 40 ng [R
Sls bo
or Abate Ha
hicedung i Wright
Ay dnorguiio trai she dilute Fo L008
BF pplionTiatinls io 20m TOC
om of ela) prosmund oH place
ino 1.080 Yyabin Phin net: erro, ok wv powh
)
=f naTrasnte poled (1.6.1.2) Dibt Nto food
ian A
A 2Ler 22 bo?! / pel tisrd(16.1.2)
Mix
soon 1
1h Wb pnd place 25000 vo sands oF Three
rpeihagn Af lade). Discancl Nesoinaing, solaliin
: Lang LF] Artur Fc-203 FP
OC: 147,000 eg [kg Sdn + Le.
HEPES [PN 40reg TOC 1,00 Rgcample 000g = 22it FC-203
3]
Dg Toc TK
Sone ale Fo Arurtrn FC:301], 107-2303 Cs
Tas adi = 40mg.
7
0
9%080 gre pT = 0.2084 Fcozo
.
9,900 mg 2 di mpl i
Lod degapnye
410008 = 2.4164 CC Lot
LY
43,405 ngTiC Chery
Hod, juno 16S YC --3 nears ZT0C 79 begunn = 2074 TC xLoog ha:xEE
Reviewedby:__0vOSS7 0.03069 = 30.6yg
J
em me er ----_--
CT .
Tent Deve.n FuCeRASHS
pits BEY (oNTROL (BLANK) . Ct cma
serieT)es A
TAR CAL BRATION
COMPOUND
- -
4A-3
ax ShmPLE
Ea
= 1-2
A
1-3
AA SAMPLE -y i2 A a3
Can StwfLe oh . 3-1 3A 3-3
boc amalopts muns ba Aurse dupthcate
Ttbuget 0,7, 14,721,418 was di dichin
#o{Doc nalyons: GtutpericheXiSoanpl=- 10 somplio
.
0 complis oralipel onduplioste 1%00sle
Seedkt
nahin Tern (o-asc (es -77F)
--- Kup navies Wighar ood A ramp.
[rs pcs(Mos .
-
TOC damdard poet Ertract arti
15s [200d
BEST COPY AVAILABLE
oe
000953
. rom socearme BEST COPY AVAILABLE
ri pote, S545
SAMPLEDESCRIPTION
ENVIRONMENTAL ENGR. LAB -- WORK SHEET
Coletti gradTod
(Doc Romer)
pOwie:rB_SE:21S:30LL--
' ----
L203
(27)
pes (1- 319 =o
41- IsY
0s = 6.5%
Deyo 1- 320- Cod) xi00 = 20.1%
He 1-9
.
TEAR
139,
Fe.23
(dog 14)
i
Fe 101
[dan2t
Dy
- (4-0. wie = 33.8%
go! ]
, 859,
De, =
Ho.2
= F - 54-1
!
dou
or 2617 J
= %9.5
L
I
--_--------
Fo o1e3
L
17)
[i 410k]
Hol
[1 daze
Yo.2
IEE py ----------
xeoz 9137
919.
Fey
(deg2t)
mp LJ) = 2.9 -0
"o-0
[i= 280
2
40.2
Jy: 9300
(yw. 930%
937.
Reviewsdby:
000959
romm toaarm
Co
BEST COPY AVALABLE
File: Pagaho, SSL S
sawpLEDESCRITION
ENVIRONMENTAL ENGR. LAB -- WORK SHEET
. K Cert make _
.
#07)
--
Dio.
pp = [1 -
:
(fs voc Lemme
308-(onD) |wieo: 220%)
G14 J pn
8.2 C0) |give: 52.29]
oH
-
r--
MEAY
4
39.7
Ti mh "-3017
>
i=30-027) 20 3
5 4,
dau 21
297
7
>
Fe -3017
40.4
U
7.7
Dey=J
I- 2.00.67] svox
oy
9.57
fete
(dag 28) D, = 1 - /5-0 ]ym0= 46
_
29.2
CT 23-0
umes 4G
nLws
-
a, 7
sh eee
000960
, Semen
BEST COPY AVAILABLE TS
neon fotos A Dopmiint ENVIRONMENTAL ENGR. LAB -- WORK SHEET
1: -200
Pa)
(0 Doc Pornrrat)
-- ra
MEAN
= {I~ 310-t-0.
--
'
er
= 033%2 237
z 1-328.8 -(-0.l,
poz 23.59
>
8-14 ]
-20
= - 5.7-04
=
A
1) !
hd
:
Jl 34 -pu Jyse0 = 92.6%
TE
90 70
Fe-206
Fg)
Ce 3-r2 Jyoos 940%
owedC ay
D =f -
ll we 44.1%
2
Ho.y
Fee Di =| lmSpm(x0 = 93.0%
day 27)
'
93%
Dey= [1-36-06 500 = 92:67:
"oy
_
FCg0b_ Dp = [1-por3 94d0 . )
Dip |1-300 0= 2 90.3] 0.)
_
0d
ee
SE
--
[--
000961
.
BEST COPY AVAILABLE
Ce
Fi panne, SS4LS
(70 briny WE ENVIRONMENTAL ENGR. LAB --WORK SHEET
PY
{Pron
ow 5:22-00
Lydesgurbns
Je)
g
be fi- 20-Con vim: esl
1.31.4)
os
Dr-[1-
y00= 90.7%
Mew
29 7%
|
,
or
rs
BL [i=7ao7 a9m7s %) 7?
Dy = [i- j-0Y soz_92.5
v
75.9
927
.
Lv e5Ges
bo dpsIpuioNns TT biz U{1-27m-a123 00 = 90h .6 97%
g
Dy )- 0.81.2
703.6%) assnsinHdawyeaso9l5,
156
Mud rguiivave
La
= [I= Jib-os
b, = 1- 19-04
>
5.6
a
(:
_--
D ==
`
%
-
2
/:0
59
08-0
5.6
pr 937 00: 19.9%
90.2%
=9
o 93
ee
ee Reedy
000962
ee OE FE EEE FE EEE EE FFE 2][TILL
rh
Hep
le Pte SE
LHe
TToSrHE z ItTg FFH r as
JF weR Hit Hr I
Lee HH
hsb
HEbhoH eee ee
hae
so
g aEe TT
Saegfen
es
A5HfcarasANPNRNNARRAEaS?
(SEEaRAEEEATRONRE]
pee sil
fi
ext t ehi sme FETE ETCITRETICNRvaEgNy his beng ETE
LE
a SE EEEL ET ALEE EeL a s LLTe L He e e Hfe ele e d e [THLE hTT
TT eT HTT
HHH
br Her ih
Sr
isin pre
ITE
ey
feE ss T mmNTAL REERERE STcEierereeaieeenIniTnTnSnypelnTEe EJTe
caatoiW b eH pR re EeeCTT e ETTe e EE e
BEST COPY AVAILABLE 000963
CT
SAMPLE DESCRIPTION
Fin: peo, 25% S
ENVIRONMENTAL ENGR. L--A WOB RK SHEET .
ow: 522-80 AWeyS r LeLfailD . ) itn
a
tewplewmb
[= (Cos duplocat
Aibutzrne)
ix
-
J -E0--strike
untrek
(Heer,
etsd
FE JS
J
EE SE JE
- I --------
JE E E" ee -
------- -
ee
ee
ee ee
:3
eee 000964
541
NviRON
4ERATORY DATA S: Caries 8Volpe ro
$
ANALTSSFORM SPAeMiPeLErin on e oe gZ A
festST pouesaevioln (Hfahfaih Cefc ff
1 feidritess
t(oasn fafrue
--Ljiaojdu aji]e} H7en7ee)
1
Ee
E
E B e Sm p r E T Ue S ot a eb tr ta e L Et a eem1e1rep tp r otioe m t1e 1 t E otoH set
B F a rapttE t e1 1t.e2r t mC 1 eTEe 7 EAet T2 eon on toe n
e I t mnE tt atltE e rea e Ee
E EI Er r Er E te -- 4 m i e e m1enet tEe t RttR o t e n tt ee ntetmn
Brr r 3 r ee 2 re aeTE anem r fee ett i E ee tE C ae eme oe 1
E M t r e re e eerie te nm Cn
2
re 1est e
B B pe ---- rt ae -- ra er-- --Rt eacr td r ve ep ssr et lsYERor EtilEotnwit r iPcacrHF t TERE r T fee nr nee l n e Ae RN e
B2E B e --m m vrReo id neer e TVe REEiceRw ACAlT BStEJEaWAEERsEpx R[eoSlREHReEdAeRASeRWN BreTIBCACCH e H 7Tm e H e m Co A anE totE A onH E
BtB were ea rom nva eks [ax3sl wie sE n E EEBrErSt iTcCoOd P)pVvAAVABILLEAB|L|E
| BE iis F e E e rr E e r | e H H er r rrr e Eereeee
|
So