Document n9Kgzk8DN1LMzaeQebpN0D52a

AR 226 -- 0873 ACUTE TOXICITY TO FISH (MUMMICHOG) IEsTsuBsTANCE 000000 Identity: ArefmeirxrteudrteocaosntPaiFnOiSng, pFeCr-f9l5u,oroorocatsanaescuolmfponoanteen,twohficFhC-m7a8y0Ba.lso(1b-e Octanesulfonic acid) (CAS # 2795-39-3). Remarks: is FC-7808, The 3M identified pbryodtuhcetliaobnorloattonruymabser"7w9a01s".notCunrorteendt. The test sample information indicates itis a mixture of 0.58% PFOS, 14% diethylene glycol monobutyl seutlhfeart,e,760..78%% wpaotleyro,xy5e.t5h%yluerneea,mo1n.o1o7c%tyHlypdhreonxyyl feotahemre,r,0.10.51%5%sosdoiduimumlaoucrtyyll sulfate, and 0.05% benzotriazole. - cThhaerafcotlelroiwziendg scounmcmeantrryataipopnlsioefsitmopuarmiitxiteusr.eDwaittha imnacyomnpoltetaeclcyurately reflect toxicity of the fluorochemical component of the test sample. M0 emo0 n.00000000000 Method: DTNSRDC Standard Static Marine Bioassay Procedure for Shipboard Chemicals. Type: Acute static GYeLaPr:coNmopleted: 1979 Species: Fundulus heteroclitus Supplier: Commerical supplier in MA. Analytical Exposure pmeorniiotdo:ri9n6g-:hoDuirsssolved oxygen, pH, salinity, and temperature. Statistical methods: LCs values and 95% confidence intervals calculated uTseisntgfaischoamgpeu:teJruvpernoiglreasm by Stephan, et al. 1978. Length and weight: Average length = 48 mm - Average weight = 1.16 g (wet) Loading: Not given Pretreatment: None. Test conditions: DDiilluuttiioonn wwaatteerr:chIenmstiasnttrOyc:ean dissolved in deionized water. SpaHl:inity: 8155ppt --- LSitgohctkinagn:dNtoetstgisvoelnu.tion preparation: The test material was added directly tgolatshse rdoidl.ution water on a weight/volume basis and mixed by stirring with a 002554 Concentrations dosing rate: Once Stabilityofthe test chemical solutions: Not noted ENxupmobseurreofverespsleilcsa:tes1:9.62 L glass jars containing 15 Loftest Numberoffish per replicate: 5 Number of concentrations: six plus a blank control Water chemistry during the study: pH range (0-96 hours): 7.1-8.4 (control exposure) 6.7 -- 8.4 (10000 mg/L exposure) Temperature range (0-96 hours): 18.5-20.0C Salinity range (0-96 hours): 1433--1177 pppptt ((c1o0n0t0ro0lmegx/pLoseuxrpeo)sure) Dissolved oxygen range (0-96 hours): - 6.87.8 mg/L (control exposure) 0.37.8 mg/L (10000 mg/L exposure) solution. REswts 00000000 Nominal concentrations: Blank control, 1900, 2800, 4100, 6000, 7700, and 10000 mg/L. Element values: 96-hour LCso = 3900 (3400 -- 4600) mg/L 96-hour NOEC = <1900 mg/L Element values based on nominal concentrations SRuebmsatraknsc:eTReesmtianrgkwsafsielcdo. nTdhucetveadlounesthreepmoirxtteudraepapslydetosctrhiabtemdixintutrhee aTnedstnot the fluorochemical proportion alone. c0 onclu0 sions0000000 `The test sample 96-hour LCso for mummichog was determined to be 3000 ~ mg/L, with a 95% confidence interval of 3400to 4600 mg/L. The 96-hour No Observed Effect Concentration (NOEC) was <1900 mg/L, the lowest concentration tested. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 p0 atA0 QuAu0 TY 0000000000 tReesltiianbgi.litHyo:weKvieirm,istchhersaanmkipnlge=pu2r.itTy hweasstnuodty pmreoepterslythcehacrriatcetreiarifzoerdquaanlditythe sintutdhye lsaolcuktsioann.alytical confirmation of the amount of fluorochemical proportion 002555 R0 EFER0 ENCE0 S 00000000 Thisstudy was conducted by EG&G, Bionomics, Wareham, MA at the request of the 3M Company, St. Paul, MN, 1979. o0 mwmeR 0000 Last changed: 6/28/00 002556 ' J ; 7%0 6 ACUTE TOXICITY OF 7901 TO THE COMMON MUMMICHOG (Fundulus heteroclitus). TOXICITY TEST REPORT SUBMITTED TO mE 30 company ) ST. PAUL, MINNESOTA REPORT #BW-79-4-416 750 Main Shree E G & G, Bionomics Aquatic_Toxicology Laboratory -- Wareham, Massachusetts ) April, 1979 002557 ) InrRoDUCTION The purpose of this study was to estimate the acute toxicity of 7901 to the common mummichog (Fundulus heteroclitus) under static conditions. Preliminary range-finding tests and a subsequent 96-hour definitive test were conducted at the Aquatic | Toxicology Laboratory of E G & G, Bionomics, Wareham, Massachu- setts. The definitive test was conducted from 20-24 March 1979. | All raw data generated are stored at the above location under | the direction of Joyce Brundage. MATERIALS AND METHODS 1) procedures used in this acute toxicity test followed those | described in "DINSRDC Standard Static Marine Bioassay Procedure for Shipboard Chemicals" (Liberatore). All values are reported | to various levels of significance depending on the measuring ] aevices employed during any one process. ] The 7901, a yellow liquid, was tested as received from the - 31 Company, St. Paul, Minnesota. Nominal test concentrations | are reported as milligrams of 7901 per liter of test solution | (mg/1) | The common mummichog (lot #7859) used in this study were ) obtained from a commercial fish supplier in Massachusetts. Upon | receipt at Bionomics, the fish were held in a 600-1 fiberglass | 002558 3 2 | tank containing a solution of Instant Ocean. This water was characterized weekly, and had a pH range of 6.5-6.7, a salinity | range of 14-18 parts per thousand (0/00) and a temperature of 20 + 1. The water was recirculated through dolomite in a biologically active filter bed to preserve water quality during ] holding and acclimation. Dissolved oxygen (DO) was maintained between 91 and 99% of saturation by this system. The pif was | measured with a Model 175 Instrumentation Laboratory pH meter and combination electrode, the temperature with a dial thermometer, the DO with a todel #57 YSI combination oxygen-temperature meter | and probe, and the salinity with an American Optical refracto- meter. Experimental animals were maintained under these conditions D tor 2 minimum of 14 days. All fish were fed a dry pelleted food, | 2d Libitun, daily except during the 48-hours prior to testing. There was 0.33% mortality during this 2 day period. ! The definitive test was conducted in 19.6-% glass jars which 1 contained 15 2 of test solution. The dilution water used in | this test vas prepared by adding Instant Ocean to deionized water and mixing until dissolved. This water had a pH of 8.5 | ana a salinity of 15 o/oo. me 7901 was added directly to the | dilution water and mixed by stirring with a glass rod. | Contzol Jars (a and 3 replicates) containing the same dilution J) water and maintained under the same conditions as test concen | trations, but containing no 7901, vere established. All test | i _ 002559 3 3 solution temperatures vere controlled by a system designed to maintain temperatures at 19 + 1C. All test solutions were gently aerated during the exposure period. Ten common mummichog (five per replicate) with a mean (range . | =30) wet weight and total length of 1.16 (0.64-2.17) grams and : 48 (40-61) millimeters, respectively, were randomly distributed | to each test concentration within 15 minutes after the solutions were prepared. . Mortalities vere recorded and removed from each test jar every 24 hours during testing. Biological observations of the fish and observations of the physical characteristics of the test solutions were made and recorded at 0, 24, 48, 72 and 96 hours. The pH, DO concentration and salinity of test solutions were . | measured at 0, 24, 48, 72 and 96 hours in the A and B replicates of the control, high, middle and low test concentrations. | Temperature was also measured in each control jar replicate at } i the above mentioned time intervals. The concentrations tested and the corresponding mortality data . derived from the toxicity test vere used to estimate 24-, 48-, 72-"and 96-hour median lethal concentrations (LCS50) and 95% mr confidence intervals. The LC50 is defined as the concentration ) (nominal or measured) of the test compound in dilution water 002560 3 4 which caused 50% mortality in the test animal population at the stated exposure interval. The computer program utilized (Stephan, 1978, personal communi cation) estimates LC50 values using one of three statistical | methods in the following order of preference: moving average angle analysis, probit analysis, binomial probability. The method selected is determined by the characteristics of the | data base (i.e. presence or absence of 100% mortality, number of partial mortalities, etc.). The computer program scans the ; data base, identifies the most preferred statistical method and D performs the analysis. | RESULTS | The 96-hour LC50 for the common mummichog exposed to 7901, esti| mated by the moving average angle method, was 3,900 mg/% | Table 1 summarizes the 24-, 48-, 72- and 96-hour IC50's and 95% confidence intervals and states the no discernible effect | concentration through 96 hours. The no effect concentration is the highest concentration tested at which there were no mortalities I or observed behavioral and physical abnormalities, e.g. loss of | equilibrium, at surface, darkened pigmentation. 2 | The pH, DO and salinity measured during the test are presented ' 002561 N 5 in Table 2. The temperature of the control solutions ranged rom 18.5-20.0C during exposure. Table 3 summarizes the nominal test concentrations and corresponding cumulative mortalities observed at 24, 48, 72 and 96 hours of exposure to each test solution. Deviations from the protocol - The common mummichog were 14.8 centimeters long - Nineteen point six liter widemouth glass bottles were used as ) test containers - Instant Ocean" solution was used for acclimation and testing - The salinity for acclimation and testing was 15 0/00 ~ Fish were acclimated in water with a pH range of 6.5 to 6.7 , and tested in water having a pH of 8.5 - Ten fish were exposed to each concentration - LC50's were calculated by Stephan, 1978, personal communication i It is our judgement that nome of the above deviations have affected the integrity of this toxicity test. i . _. J i 002562 3 LITERATURE CITED APHA, MHA, WECE. 1975. Standard methods for the examination ; Of water and wastevater. 14th Bdition, Washington, D.C. I 1193 pp. . Liberatore, Giacomo. David W. Taylor Research andDevelopment | Center, Standard Static Marine Bioassay Procedure for i Shipboard Chemicals. Report TM-28-76-29. i Stephan, Charles. 1978. U.S. EPA Environmental Research ) Laboratory, Duluth, Minnesota. Personal communication, |i 002563 SUBMITTED BY: I) : PREPARED BY: EG&G, Bionomics Aquatic_7T90oxMiacionlogSytreLeatboratory WarehaAmp,rilM,ass1a9c79husetts Robert J. Buccafusco i PRINCIPAL INVESTIGATOR: Study Director - : Charlotte Stiefel : PROGRAM COORDINATOR: George A. Cary | i DATA AUDITED BY: :! J | Director, Aquatic =x - Ronni Krasny . ALdambionriasttorrayfZoPrr,acticZecodas 002564 } |3 TEST CONDUCTED BY: ! `| D | : Charlotte Stiefel Assistant Biologist Diane M. Sullivan Quam. Stlivan Biological Assistant | P ! 002565 ---- = = --- = -- = ----- = Table 1 = The 24-, 48-, 72- and 96-hour LCSD values and 95% conidence intervals for the common mumichog (Fundulus heteroslitus) exposed to 7901. -- 0 ou"ws 9 hourom 72 hous e36m how | WA5.I60000 (2,45,0160-05,700 (23,,4096040,500) (33,,49600004,600) tehrroeYucgothdci5o6snccenreonnwirtssrlaes(i9o/n0) <,900 SEoattiinnaatteedd by by the the binonial probability moving average angle method. method. a3L5owesctoncfoindceenncteraitnitoenrvatless.ted. : . . 8 N&a3 . Tie 3 - The i 50 a8 anbiney ssssoreents ned dieing 2 cb conic D determination with 7901 and the common mummichog (Fundulus heteroclitus). --_-- i co`mNTmoWmnAiinTnalton oh mew ene mer seo re pu 10,000 8.4 8.0 7.7 6.7 Toe. | [wt (mg/2) i control mem 6,000 om 1,900 ot ree? 7.8(98) aoa 7.8(98) 6 esse 0.6(7.5) eae 3.6(45) 2a esas 0.5(6.3) coum 3.1039) 6 exes 0.3(3.8) case 6.3(79) 7 osso 0.6(7.5) va0m 6.2(78) J ) salinity? 10,000 15 17 15 14 13 {oo} 6,000 15 17 15 14 13 | contre EE - .n .ieescements made tn the A n sepbicte sctotins. n ] + of saturation at 19C. - J 002567 y Table 3 -- Concentrations tested and corresponding cumulative mortalities of the common mummichog (Fundulus heteroclitus) exposed to 7901 at 24, 48, 72 and 96 hours. Ten fish were exposed to each concentration, five fish per replicate. Ve oe !| conNcoemnitnraaltion Je-Cumulativemortalities(4and 3replicates) i (mg/2) our our = our 10,000 7,700 6,000 ) 4.100 2,800 1,900 | control 10 10 acide odie o%re o 0 10 10 10 icf Pre o 0 ET 10 10 pice bres obrcre 0 10 10 10 bicig pt] o 0 J Solutions had foam on the surface throughout exposure. | Pselutions were cloudy. "some fish were at the surface. %Sone fish showed a loss of equilibrium. | rien were respiring rapidly. Some fish were gulping. ! "rish vere lethargic. J i i . 002568