Document n9D3rovZOb6dgowDnZ906G5NG

AR226-0522 MICROBICS MICROTOX TOXICITY TEST TEST SUBSTANCE Identity: Perfluorooctanoic acid, ammonium salt; may also be referred to `as PFOA ammonium salt, Ammoniumperfluorooctanoate,PFO, FC-116, FC-126, FC-169, FC-143 or as the major component of FC-118. (Octanoic acid, pentadecafiuoro-, ammoniumsalt, CAS # 3825-26-1) Remarks: The test sample is FC-118. It's purity was notsufficiently characterized, though current information indicates is 20% FC-143 in 80% water. The 3M product lot number was not noted. The following summary applies to the test sample as a mixture of the tceosntcesnutbrsattainocnes ionfwiamtpeurristoileus.tioDnatwaitmhaiyncnoomtplacectuerlaytcehlayrraecltaetreitzoexdicity of the test sample with that of the test `substance. METHOD: Method: Microbics Microtox "BASIC" Procedure Test type: Static acute GLP: No Year Completed: 1996 Species: Photobacterium phosphoreum Analytical monitoring: pH and osmotic condition Exposure period: 30-minutes Statistical methods: EC50 values calculated by statistical linear Treegsrtesosrigoannpirsomgrsaomurpcreo:viMdiecdrofboircsM'icRreoatgoexnt. Test conditions: Stock Diluent: and test MsiollluiptoiroenMpirlelpia-rQaTMtwiaotn:erA primary 10 g/L stock solution was was prepared in Millipore Milli-QTM water. 6.0 (no adjustment) and an osmotic The pH of the adjustment was stock solution made using 200 mg NaCl as "clear in 10 mL stock and solorless". solution. Appearance All test solutions were of test made solutions was noted by proportional dilutions. Exposure vessels: 3 mL Cuvettes. Number of replicates: two. Number of concentrations: Four plus a blank control Element basis: Percent light loss. RESULTS 04028 Nominal concentrations: Blank control, 625, 1250, 2500, and 5000 mg/L Element value: 5-minute ECso = 4460 (4020 -- 4950) mg/L 15-minute ECso = 3360 (3090 -- 3641) mg/L. 30-minute ECso = 3150 (2910 -- 3420) mg/L. Element values based on nominal concentrations. Remarks: Testing was conducted on the mixture of the test substance as described in the test substance remarks field. The values reported apply 1o that mixture and not the test substance. CONCLUSIONS The FC-118 30-minute ECs, for Photobacterium. phosphoreum was determined to be 3150 mg/L with a 95% Confidence Interval of 2910 to 3420 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, MN, 55133 DATA QUALITY. Rteesltiianbgi,libtuyt: lKaicikmsiasncahlyrtainckalincgon2f.irTmeasttiionngomfeteetstssaullbcsrtiatenrciaefocorncqueanltirtaytions. There is a lack of characterization of the test sample. REFERENCES This study was conducted by the 3M Environmental Laboratory, St. Paul, Minnesota, Lab Request number P1626. OTHER Last changed: 5/25/00 04029 : ENVIRONMENTAL LABORATORY: MICROTOX PREPSIEET De PtlaTca Pamie ee TeesTsUBe STANGE:eFr C AAeItTe-------- " r 1 Preparationoftock Slaton FMatssDeilltToensSvuoblsuimaensee T1. 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