Document mqoKv4mqp0zDLM5nRJQKzBBbd

LYMPH NODE BIOPSIES BONE MARROW CORE BIOPSIES 0.2-1 cm3 tissue from the middle of the lymph node and out it into transport culture medium or 4-6 touch-preps. (2nd priority) ! FISH Assays Tissue Banking and Molecular Studies (3rd priority) Cut into several 3 mm-thick sections (1st priority) j If bone marrow tissue core is > 2.5cm long, cut in half. Place half in culture medium for FISH and fix half. Submit 2 or more sections for routine fixation (10% neutral buffered formalin, 27 C, 18 hours) If < 2.5cm long, fix the whole specimen in 10% neutral buffered formalin at 27C, 18 hours Wash in 85% Alcohol, 20 seconds Dehydration and wax-bath in a Shandon PathcentreTM Embedding, 65C HFISH or DNA- Paraffin-embedded blocks based Assay Sectioning (3-4mm thick sections) Special stains De-waxing and re-hydration t I IH.E. Staining Immunohistochemical stainings Microscopic examination 73 SHELL-MCCLURG-059791