Document mqXqnmvKJnQG3nJm3pn56dVLQ

ARY16-0525 ACUTE TOXICITY TO FISH TEST SUBSTANCE Identity: Perfluorooctanoic acid, ammonium salt; may also be referred to as PFOA ammonium salt, Ammonium perfluorooctanoate, PFO, FC116, FC-126, FC-169, FC-143 or as the major component of FC1015. (Octanoic acid, pentadecafluoro-, ammonium salt, CAS # 3825-26-1) Remarks: The test sample is FC-1015. It's purity was not sufficiently characterized, though current information indicates is a 30% straight carbon chain version of FC-143 in 80% water. The 3M product lot number was "HOGE 205". The following summary applies to the test sample as a mixture of the tceosntcesnutbrsattainocnes in of wIamtpuerristoielsu.tioDnatwaitmhaiyncnoomtplacectuerlaytcehlayrraecltaetreitzoexdicity of tchoempteasrtesatomxpilcietywiotfhtthheatbroafntchheedt/essttrsauibgshttacnhcaei.n Data was used ammonium to pertluorooctanoate homologue mixture in FC-143 vs. what is supposed to be the 100% straight carbon chain ammonium perfluorooctanoate in FC-1015. METHOD: Method: USEPA -- TSCA 1993. 797.1400 Type: Static acute GLP: No YSpeeacriceosm:pPliemteepdh:al1e9s96promelas SAnuaplpyltiiecra:lAmqounaitticorBiinogs:ysTteemmpse,raFtourtreC,ollpiHn,s,coCnodluoctriavdioty, and DO Exposure period: 96-hours Statistical methods: LCs, values calculated using the Stephan computer program, 1983 Test fish age: Juvenile Length and weight: Average length = 35 mm Average weight = 0.36 g (wet) Loading: 0.24 g fish/L Pretreatment: None. Test conditions: Dmigl/uLti(oans wCaateCrO:)Deainodnipzaesdsweadttehrraodujguhstapeadrttoicaulhaatrednfieltsesr, ouflt4r0av--iol4e8t wv 9 28 020 s sterilizer, and activated carbon. DilutioHnawradtneerssc:hemistr4y4:mg/L as CaCO Alkalinity: 29-30 mg/L as CaC . 02049 ToC: <1 mglL Lighting: Cool-white fluorescent lights at 30 foot-candles. A daily photoperiod of 16 hours light and 8 hours dark with a 15 minute transition period was maintained throughout the testing period. Stock and test solution preparation: Test solutions were created by direct individual weight additions. Concentrations dosing rate: Once Stability of the test chemical solutions: Not noted Exposure vessels: 20 L glass aquaria containing 15 liters test solution at an approximate depth of 18 cm. Number of replicates: two Number of fish per replicate: 20 Number of concentrations: five plus a blank control Water chemistry during the study: Conductivity range: (0-96 hours) 180 -- 190 ymhos/cm (control exposure) 300 -- 320 pimhos/cm (2100 mg/L exposure)* pH range (0-96 hours): 7.37.9 (control exposure) 7.27.7 (2100 mg/L exposure)" Temperature range (0-96 hours): 2212..80 --2222..45 CC ((c2o1n0t0romlge/xLpoesxuproes)ure)* Dissolved oxygen range (0-96 hours): 5.89.2 mg/L (control exposure) 57-92 mg/L (2100 mg/L exposure)" + 2100 mg/L (second highest concentration) exposure values used because the highest concentration used resulted in total mortality at 72. hours. B0 ESOL0 TS 000000000O0 OO0 00 Nominal concentrations: Bk control, 530, 830, 1330, 2100, and 3330 mg/L Element values: 96-hour LC50 = 2470 (2100 -- 3330) mg/L. 96-hour NOEC = 830 mg/L. Element values based on nominal concentrations Remarks: Testing was conducted on the mixture of the test substance as described in the test substance remarks field. The values reported apply to that mixture and not the test substance. CONCLUSIONS The test sample 96-hour LCs, for fathead minnow was determined to be 2470 mg/L with a 95% confidence interval of 2100 to 3330 mg/L. 04050 Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Klimisch ranking 2. Testing meets the criteria for quality testing. However, sample purity was not properly characterized and it lacks analytical `confirmation of test substance concentrations. REFERENCES Test was conducted by T.R. 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ASTM. 1986. standard I Practice for SI Conducting Acute I Toxicity Tests with ren me wee re oe se St emt Ba bs En : aw0 sa ak CoLa ga E vos oi RE La E re a oo BLA x SONHEAg 8SE HL p4066 BESTCOPY AVAAIBLIE pte Td. ER EEHLF cosa i as Winns cn ! f LsrR i TO Qr poisr sive Teiy ioga oRsda Qe ithe R EEE gulhe ap Ee i Sitges BBbeainnUGE E %BvaH. g a ie HE ns EN Toute guises aE Rama atin ah ERER a RR RibaBa ; PER sv BESTCOPY AVAII ABLE * Appendix A. WATER QUALITYDATAFROMTOXICITYTEST fai WE UIE be aE Eo ER iy sty wm sim RE He gent (#8 gi ihe Ce i Ri ls i i HE aE at ud ubae lfa asrsietcoviyecoet mtat,esi2one ,O_Yconarr iviey, on,Wea]nd TP ronmwm. EvTL H iH mP c mwE yeioopv mEZweznZr wE Clem P1 a23aTme a5am6a8 n60 mle aWe mle mwe awe " sa es 63 ea sa m0 se aw a0 me a pMammn mEmnEn | - Pomme mE mN me Sliear. Sa L Clon E B pol E ERRae ai fLdagSa oHEt Ls EARTR 4Lo CERRER RI RR RA 4S aCwlimiry study member 10317u fC 8 Bi. 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