Document mqVz07oQxqMOE0L0dpMVw7Jz4

07070 ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE_______________________________________________ Identity: Mixture containing perfluorooctanesulfonate which may also be referred to as PFOS, FC-95, or as a component of FC-201F. (1-Octanesulfonic acid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8heptadecafluoro-, potassium salt, CAS # 2795-39-3) Remarks: The 3M production lot number was 1. The test sample is FC-201 F (F-11129). Current information indicates that FC-201 F is a mixture of 3.0% PFOS, 36.0% diethylene glycol butyl ether, 36.6% water, 8.25% acrylic foamer, 7.0% sodium octyl sulfate, 6.0% sulfonated alkyl ether sodium salts, 3.0% triethanolamine, and 0.15% tolyl triazole. The following summary applies to a mixture with incompletely characterized concentration of impurities. Data may not accurately reflect toxicity of the fluorochemical component of the test sample. METHOD:________________________________________________________ Method: OECD Guideline 203. Test type: Acute static GLP: No Year Completed: 1992 Species: Pimephales promelas Supplier: ECT, Superior, Wl, maintained at AScl-DETD, Duluth, MN. Analytical monitoring: Temperature, pH, DO, and conductivity Exposure period: 96-hours Statistical methods: LC50 values calculated using the Trimmed Spearman-Karber method. Test fish age: Juveniles (~30 days) Length and weight: Length = 0.8-1.6 cm with a mean of 1.1 cm Weight = 0.001-0.044 g with a mean of 0.013 g Loading: 0.13 g/L Pretreatment: Not noted. Test conditions: Dilution water: Shallow well water from the Two Harbors, MN area. Dilution w ater chemistry: Hardness: 155 mg/L (as CaCOs) pH: 8.25 Lighting: Cool-white fluorescent lights providing a daily photoperiod of 16 hours light and 8 hours dark. 000331 Stock and test solution preparation: All test solutions were prepared by direct additions. Concentrations dosing rate: Once Stability of the test chemical solution: Described as stable. Exposure vessels: 4.5 L rectangular glass tanks (20 cm L x 15 cm W x 15 cm H) with 1 liter of test solution. Number of replicates: two Number offish per replicate: 10 Number of concentrations: 5 plus a blank control Water chemistry during the study: Conductivity range (0-96 hours): 346 - 371 pmhos/cm (control exposure) 396 - 437 pmhos/cm (1000 mg/L exposure) pH range (0-96 hours): 8.24 - 8.41 (control exposure) 7.86 - 8.36 (1000 mg/L exposure) Temperature range (0-96 hours): 20.7 -2 1 .5 C Dissolved oxygen range (0-96 hours): 7.4 - 8.6 mg/L (control exposure) 4.6 - 7.9 mg/L (1000 mg/L exposure) RESULTS__________________________________________________ _ Nominal concentrations: Bk control, 120, 204, 346, 588 and 1,000 mg/L Element value: 96-hour LC50 = >1,000 mg/L 96-hour NOEC = 588 mg/L Element values based on nominal concentrations. Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The value reported applies to that mixture and not the fluorochemical proportion alone. C O N C L U S IO N S __________________________________________________ The FC-201F 96-hour LC50 for Fathead minnow (Pimephales promelas) was determined to be >1,000 mg/L. The 96-hour No Observed Effects Concentration (NOEC) was 588 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY__________________________________________________ 000332 Reliability: Klimisch ranking 3. Dissolved oxygen fell below an acceptable level at 24 and 48 hours for the highest test concentration in one replicate. The range finding test results did not agree with the definitive test. The range finding test resulted in 100% mortality by 24 hours at 1,000 mg/L, whereas the definitive test had only 30% mortality by 96 hours at 1,000 mg/L. Testing was redone to determine if there is a relationship between the surface area of the exposure chamber to the volume of the dilution water / test solution. The sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in solution. R E F E R E N C E S _______________________________ _____________________ This study was conducted by AScI Corporation, Duluth Environmental Testing Division, Duluth, MN at the request of th&3M Company, Lab Request number K1792, 1992. OTHER___________________________________________________ Last changed: 6/26/00 000333 AScI Cocponlioo/AScI-Duiulh AESncAvIiSRrocenIpmSoterutndItyDalI#TD0e#1st55in-0Fg3H0D-A0iv.1Ri5s3i-o0Mn3 STUDY TITLE ACUTE TOXICITY OF FC-201F TO FATHEAD MINNOW (PIMEPHALES PROMELAS) DATA STANDARD OECD GUIDELINE 203 STUDY COMPLETED August 24, 1992 TESTING FACILITY AScI Corporation AScI-Duluth Environmental Testing Division 112 East Second Street Duluth, MN 55805 Tel. No. (218) 722-4040 STUDY IDENTIFICATION NUMBERS AScI Study ID# 5030-015-03 3M Company Lab Request# K1792 SSppoonosioorr.S3tuMdyCIoDm#pKanIy792 1 000334 EAnSvicrIonCmorepnotarallTioeas/tAinSecID-Divuishioitnh AScAI SRceipSotrutdIyD#ID0#155-0F3H0-A01.R53-0M3 1.0 GENERAL INFORMATION Study Title Data Standard Sponsor Sponsor's Representative Sponsor's Lab Request# Testing Facility Principal Investigator Project Director Testing Facility Director Definitive Test Dates Data Validation and Report Review Retention of Raw Data and Final Report Location of Raw Data and Final Report Acute Toxicity of FC-201F to Fathead Minnow (Pimephales promelas) OECD Guideline 203. 3M Environmental Laboratory, Building 2-3E-09, 935 Bush Avenue, St. Paul, MN 55106. Susan A. Beach, 3M Environmental Laboratory, Building 2-3E-09, 935 Bush Avenue, St. Paul, MN 55106; Tel No. (612) 778-7452. K1792. AScI Corporation/AScI-Duluth Environmental Testing Division, 112 East Second Street, Duluth, MN 55805; Tel. No. (218) 722-4040. Jo A. Thompson Dinesh Vaishnav Donald Mount July 9-13, 1992. Dinesh Vaishnav Zjwfu Report Signature: Two years from the date study is completed. AScI Corporation/AScI-Duluth Environmental Testing Division, 112 East Second Street, Duluth, MN 55805; Tel. No. (218) 722-4040. Sponsor 3M Company Sponsor Study ID# K1792 2 000335 2.0 OBJECTIVE(S) AESAncASvIicSrRIocenCipmSootieruptndoItysDn!t#IiToD0eo#1s/At55in-0SFe3cHI0D-A0Di1v.Ru5iih3-io0iMt3ah To determine the 96-h LC50 and 96-h NOEC (no observed effect concentration) of the test substance, FC-201F, for fathead minnow (Pimephales promelas) under static test conditions. This study was conducted according to the AScI study plan retained by the Sponsor and OECD Guideline 203 (OECD 1984). 3.0 TEST METHODS Test Substance Test Organisms Properties: FC-201F, orange/red liquid, relative density 1.1 (water = 1), stable, and miscible with water. Test concentrations: 0.0 (dilution water control), 120, 204, 346, 588, and 1000 mg/L, prepared by direct additions. By 24 h, all test* solutions, except the control, were foamy. Source: Healthy, juvenile fathead minnows (Pimephales promelas) obtained from a commercial supplier. Holding/acclimation: Held and acclimated for 15 days, during which exposed to the dilution water for 14 days. 0% mortality occurred during holding/acclimation period. At test termina tion, they were 0.8-1.6 cm in length and 0.001-0.044 g in weight with a mean of 0.013 g. Use in test: Did not feed 24 h before the test initiation. Used 10 organisms/replicate and 2 replicates/test concentration. SSppoonnssoorrS3tMudyCIoDm#pKan1y792 3 000336 EAnSvcirIonCmorepnotanltToeoi/tAinSgcID-Divuihikitnh AScI Report ID# 015-FHA.R3M AScI Study ID# 5030-01J-03 Dilution Water Exposure Chambers Incubation Observations Endpoint Calculations Source: Shallow well water from the Two Harbors, Minnesota area. The most recent chemical analysis is in Raw Data Package. Characteristics: At test time, the water had a hardness of 155 mg/L (as CaC03) and a pH of 8.25. Aerated for 24 h prior to using in the test. Type: 4.5-L rectangular glass tanks, each measuring about 20 cm length X 15 width X 15 cm height. Use in test: 1 L of dilution water or test solution/chamber. During the test, kept covered, except when experimental observations were made. Gentle aeration was provided throughout the test. Duration: 96 h. Daily photoperiod: 16 h light and 8 h dark, maintained using cool white fluorescent lamps. Temperature: 20.7-21.5C. Biological: 3 h, 6 h, and daily- Test organisms with abnormal behavior and signs of stress, and dead test organisms. Removed dead test organisms when observations were made. Water chemistry: (1) At test initiation- total hardness and alkalinity, (2) dailytemperature, DO, and pH, and (3) at test initiation and termination- specific conductivity. All determinations were made according to APHA methods (1989). LC50: Trimmed Spearman-Karber method (Hamilton et. al. 1977). NOEC: Dunnett's procedure (TOXSTAT statistical software, Version 3.1, University of Wyoming 1989). Spooior 3M Company Sponaor Study DD#K1792 4 00033 3.0 RESULTS EAnSvicrIonCmorepnotanlliToena/rAinSgcID-Divuiikioitah AScAl SRceIpSotrutdEyDI#D0#1J5-0F3H0A-0.1R53-0M3 Test Substance Toxicity 24-h LC50: >1000 mg/L. (Pertinent data are in Table 1) 48-h LC50: >1000 mg/L. 72-h LC50: >1000 mg/L. 96-h LC50: >1000 mg/L. Ranges of Water Chemistry Parameters 96-h NOEC: 588 mg/L. Total hardness (as CaC03 mg/L): 155-160. Alkalinity (as CaC03 mg/L) : 125-140. Temperature (C): 20.7-21.5. DO (mg/L): 4.6-8.0. pH: 7.86-8.54. Specific conductivity (ftmhos/cm) : 346-437. 4.0 TEST QA/QC QA/QC Criteria Mortality in control exposure must not be more than 10%. The lowest DO concentration at test termination must be at least 60% of the air saturation value at test temperature. Test duration must be 96 h. Reference Substance Toxicity Test (this test is optional). Data 0% mortality. The lowest DO concentration measured was 53% of the air saturation value at 21.0C. The test duration was 96 h. Final report for the most recent test with sodium chloride is in Raw Data Package. Sponsor 3M Company Sponsor Study ID# K1792 5 000338 5.0 DEVIATIONS EAnSvicrIoCnmorepnotanltiToeas/AtinSgcID-Divuiliuiatha AScAI SRceIpSotrutdIyD#ID0#155-0F3H0-A01.R53-0M3 1. The DO fell below 60% of the air saturation at 24 h and 48 h in one replicate of the highest test concentration. _____ The dead fish were removed and aeration was adjusted. 6.0 ADDITIONAL STUDY In the range-finding test, which was conducted using 1 L dilution water in 1-L borosilicate glass beaker, 100% mortality occurred by 24 h at 1,000 mg/L test substance. However, in the definitive test, which was conducted as described before, only 30% mortality occurred by 96 at 1,000 mg/L test substance. As a result, the range-finding test was repeated and the data showed that by 3 h 100% mortality had occurred at 1,000 mg/L test substance. The information for the repeat test is in raw data package.____________ 7.0 REFERENCES American Public Health Association (APHA). 1989. Standard Methods for the Examination of Water and Wastewater. APHA, Washington, D.C. Hamilton M.A., R.C. Russo and R.V. Thurston. 1977. Trimmed Spearman-Karber method for estimating median lethal concentration in toxicity bioassays. Environ. Sci. Technol. 11:714-719. Organization for Economic Cooperation and Development (OECD). 1981/1984. OECD Guidelines for Testing of Chemicals. OECD Publication Information Center, Washington, D.C. University of Wyoming. 1989. Toxstat Version 3.1. Fish Physiology and Toxicology Laboratory, Department of Zoology and Physiology. University of Wyoming, Laramie, WY. SSppoonnssoorrS3tuMdyCIoDm#pKan1y792 6 000339 EAnSvicrIoCnmorepnotaral tTkens/tAinSgcID-Divuishioitnh AScI Report ID# 01J-FHA.R3M AScI Study ID# 5030-013-03 Table 1. Acute toxicity of FC-201F to fathead minnows (P. promel&s) Test substance nominal concn (mg/L) 0.0 (control) 120 204 346 588 1,000 Cumulative number of dead organisms and % mortality* 3h 0 (0) 0 (0) 0 (0) 0 (0) 1 (5) 1 (5) 6h 0 (0) 0 (0) 0 (0) 0 (0) 1 (5) 1 (5) 24 h 0 (0) 0 (0) 0 (0) 0 (0) 1 (5) 5 (25) 48 h 0 (0) 1 (5) 0 (0) 0 (0) 1 (5) 5 (25) 72 h 0 (0) 1 (5) 0 (0) 0 (0) 1 (5) 6 (30) 96 h 0 (0) 2 (10) 1 (5) 1 (5) 1 (5) 6 (30) `Percentages are in parenthesis. LC50 and NOEC 24-h LC50 48-h LC50 72-h LC50 96-h LC50 96-h NOEC Effect and no-effect concentrations with corresponding 95% confidence intervals Data (mg/L)1' >1000 >1000 >1000 >1000 588 95% Confidence interval not calculable not calculable not calculable not calculable Not applicable bLC50 values were calculated based on the test substance nominal concentration. 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