Document mqBXDkx238DaxMOYZ7e0L1MVZ

o o o o ED 002061 00090190-1 Tier 10 ENST PhyloChip Technology Development Advanced DNA profiling ORD, UC-Berkely, OECA, USGS, Multi Regional, State / Tribal Collaboration Year 2 of 5-year project timeline Simultaneous analysis of 1.1M DNA sequences for 58k different bacteria IDs At present: Qualitative Presence / Absence determination Ultimate Goal: R7 will be the only other lab with this capability beside UC-Berkely nationwide Bacteria Source Tracking Harmful Algal Blooms PhyloChip can differentiate between HABs are actually bacterial blooms Human sources Dog / Cat Bird Horse Pig Ruminant (Cow, deer, elk, etc.) Toxins affect drinking & recreational waters Genetic information collected to help understand conditions favorable to HAB formation, toxin release, and control strategy development 800 Cyanobacteria IDs Next Steps: Continue method validation against reference methods Continue development of R7 capacity / capability Continue working with UC-Berkely to develop chip specific to environmental analysis Refine method to improve quantitative ability Move from contract to in-house capability for all EPA (Currently Regions 1,4,6,7,10) Sierra Club v. EPA 18cv3472 NDCA m VcuS &as Sierra Club v. EPA 18cv3472 NDCA Tier 10 ED 002061 00090190-00002