Document mpQ969vOdn1yoxy8v0r3nwdkZ

K-/7//-C/CWJ ^ M6Cp* ' 80-5-21 ' W y[frC Gigiena Truda I Professional 'nye Zabolevaniia V. (.3) 46-7 (1980) Salnikova, L. S,, Kitsovskaya, I. A. EFFECT OF VINYL CHLORIDE ON EM3RYOGENESIS IN THE RAT Institute of Hygiene and Occupational Diseases, Academy of Medical Sciences, U.S.S.R. R&S 133819 The fact that there are widely scattered throughout the U.S.S.R. many plants involved in the production of vinyl chloride (VC), poly vinyl chloride (PVC) and in the manufacturing of them into finished articles and that there is a significant quantity of workers who are women makes it necessary to study the embryotropic effect of the said compound. Vinyl chloride in high concentrations together with ethanol produces an embryotoxic and teratogenic effect (Zohn et al.). The purpose of our investigation was to study the embryotropic effect of VC in an experiment in concentrations on the level of those existing in the present MAC and those on the order below the MAC. METHOD. The embryotropic effect of VC was studied in rats of the Wistar population. The animals were subjected to the inhalation effect of the toxin for the full course of pregnancy for four hours per day in conditions of dynamic inoculation. The gas content in the inoculation chambers was 35.5 +3.5 mg/m 3 3 +0.5 mg/m (on the level below the MAC). (MAC level) and 4,8 Beginning with the sixteenth day of pregnancy the rats were monitored for a series of integral and special indicators. In order to clarify the harmful effect of VC on the fetus, a / 7 7> R&S 133820 2 portion of the rats was sacrificed on the twentieth day of pregnancy. The pre and post implantation death rate and also the general embryo death rate were calculated. The pathology of the internal organs of the fetuses was studied in a series of micro scopic sections (Wilson method modified by A. P. Dyban et al.). In connection with the fact that the development of defects frequently appears in the postnatal period, a portion of the rats was allowed to go on and give natural birth. As this occurred, a calculation was made of the survival and lactation coefficient. Beginning with the age of 3 days a test was made of the induced motor activity in the offspring, and when they were 2 months old a test was made of the food conduct reaction. As indicators which characterize the induced motor activity of the offspring of the rats of differing age, we used the time the animals spent on a horizontal cord, the time they spent on a vertical cord of 3 diameters (11, 17 and 25 mm), various elements of motor reaction on the vertical cord (Altman and Subarashan). In all, 230 analyses were conducted. Specialized forms of appetite, general appetite and thirst were used as "food behavior" indicators. At the age of 6 months the offspring were investigated (with respect to gender of the animals) for a series of indicators which reflected the function of the nervous system, liver, kidneys, a study was made of the morphological composition of the peripheral blood, a calculation was made of the weight of the internal organs. In the experiment a total of 130 pregnant rats were used, an analysis was conducted on 230 of the offspring, produced and 3 found during observation of 700 specimens of offspring. INVESTIGATION RESULTS. The results of studying the general toxicity of VC are presented in the table. It was established that VC in concentrations on the level of those existing in the MAC in the pregnant animals causes a decrease in erythrocyte content in the blood and a lowering of the hippuric acid in the urine. In reactions of the toxin at concentrations one order of magnitude less than the MAC, with respect to all of the indicators selected, no deviation could be shown in comparison to the controls. At the above-indicated concentrations VC had no negative effect on the pre and post implantation and general embryonic death rate. Tests on the fetuses conducted by the microanatomical method showed an increase in the relative number of them with hemorrhages (51.2 +6.3%; P< 0.001 under the effect of the greatest concentration; 49.3 +8.7%; P< 0.001 under the effect of the average concentration; 12.3 +3.2% in the control). Under the effect of VC at a concentra tion of 35.5 mg/m^ there was also noted an increase in fetuses with intumescence (62.0 +7.9% with 4.2 +3.2% in the control; P< 0.001). The effect of VC on pregnant rats at concentrations of 35.3 and 4.8 mg/m^ did not lead to a change in such indicators as lactation coefficient and offspring survival coefficient. An analysis of the reactions conducted in the offspring which during the antenatal period were subjected to the effect of VC in the larger concentration showed an increase in the retention time of the 9-11 day old rats on the vertical cord (98.3 +8.49 s with 23.8 +3.88 s in the control; P <0.001). In this very same group a R&S 733821 4 larger number of those which remained on the vertical cord for 1 minute was noted (.62.5% with 10.4% in the control) and there were practically no cases of them jumping off of it (54.2% in the control). The 9-11 day old offspring subjected to the effect of VC in the antenatal period at the lesser concentration were able to stay on the horizontal cord for a more prolonged period of time (24.2 +2.7 s with 16.9 +1.47 s in the control; P<0.05) there were no cases of retention on the vertical cord for 2 minutes. Investi gations of the specialized form of appetite, general appetite and thirst in the 3 month old offspring in the test groups did not show any reliable changes. Investigations in the 6 month old offspring showed changes in the function of the nervous system, liver and of the composition of the blood. Thus, for example in the offspring of the male gender of the first test group a lowering of the orientation reaction was noted (2.1 +0.41 arbitrary units with 4.1 +0.5 arbitrary units in the control; P <" 0.01) , total subthreshold impulse (4.7 +0.3 arbitrary units with 5.6 +0.31 arbitrary units in the control; P< 0.05), a lowering of the hemoglobin in the blood (13.1 +8 g/1 at 169 +7.6 g/1 in the control; P< 0.01), a lowering of the weight coefficients of the heart, liver, kidneys and spleen. In the female offspring of this very same group a lowering was noted of the leucocyte content in the blood, an increase in quantity of hippuric acid in the urine, an increase in hexenal sleep time, a lowering of the weight coefficient of the heart. R&S 133822 5 THE RESULTS OF THE INVESTIGATION OF PREGNANT RATS SUBJECTED TO THE EFFECT OF VC AT CONCENTRATIONS OF 35.5 AND 4.8 mg/m3 an oKJwrc.ifc ecnn. yc.i. m. fKopKoawfi petf-ieKC. yc-1. ej. gOpHeHTMpCBOSiiaa peaKuKH, yc.i. e* fiPeKTa.Ti.KaH Te.\inepaTypa, C iCJA. yc.i. ej. iCcuep/KaHHe Hb. r/.i fcSpHTpOUHTbl. 1011 .1 IjlCHKOUHTU 10* .1 mTHnnypoeas Knc.iora, .vr/.M.i njHype3, m.i C6e.ioK b woNe, wr/.i pX.iopiubi a Mone, mmo.ik O^tacca Tejia. r U<oa4><iJH1iHeHT .\iac.cw BHyTpeh hhx op raHoa: SMoar tcepAUe U.ierKKc VneieHi. WnOMKH Xce.ieaeHKa C KOH TpO.*kM j n (n-II) 4,9*0.14 5.8*0.57 7,9+0,86 36,4*0.12 66.6*9.9 125*5.4 4.9*0.15 12.8*1.4 145.2*15.0 5.7*0.76 5750*760 0,05*0.004 342*6.7 0.50*0.02 o^g-^o.oos 0,55*0.01 4.70*0,2 0.54*0,01 0,40*0.02 ^ fpyn ni 4.1*0.12 7.5*1.54 11.2*1.03 36.8*0.09 53.5*12.9 132*3.2 4.8*0.22 11.0*0.1 139.6*10.8 4.5-f-0.53 5590*1040 0,05*0.006 347*10.3 0.49*0.02 O,23*0,00G 0.56*0.01 4.97*0.25 0,57~t-0,0l 0.41*0.03 S tM> 1 ------ a-1 35.5 ki-/m* In -- 10) 4.7*0,1 7.60.92 10.2*1,04 36.7*0.1 84.6*12.4 128*5.2 3.9*0.33 P< 0.02 11.4*0.84 96.2*12,9 P<0.05 6.7*0.55 4450*430 0.04*0.003 313*13,4 0.57*0.03 0.33*0.01 0.62*0.03 5,06*0,12 0,60*0.02 0.51*0.05 a - Indicator b - Group c - Control d - mg/m3 e - Total subthreshold impulse, arbitrary units f - Cortical reflex, arbitrary units g - Orientation reaction, arbitrary units h - Rectal temperature, C i - "SDA", arbitrary units j - Content of Hb, g/1 k ~ Erythrocytes, 10-*-2 1 1 - Leucocytes, 10^ 1 m - Hippuric acid, mg/ml n - Diuresis, ml o - Albumin in the urine, mg/1 p - Chlorides in the urine, mmole q - Body weight, g r - Weight coefficient of the internal organs; s - Brain t - Heart u - Lungs v - Liver w - Kidneys x - Spleen 33 9? (/> CO 03 CD 40 03 6 In the investigation of the offspring of the second test group (4.8 mg/m^) a whole series of changes also showed up. In the males a lowering of the hemoglobin was noted (146 +8 g/1 with 169 +7.6 g/1 in the control; P = 0.05) and a lowering was noted of the content of leucocytes in the blood, an increase in the content of hippuric acid in the urine (141 +14.8 mg/ml with 88.3 +9.4 mg/ml in the control; P< 0.01), a change in the weight coefficients of the liver, kidneys, and spleen. In the males of this group a drop was noted in the erythrocyte and leucocyte content in the blood, an increase in the hippuric acid content of the urine and a drop of weight coefficients of the lungs and liver. CONCLUSION. 3 MAC (35.3 mg/m ) VC at concentrations at the level of the existing in a reaction during the course of the total pregnancy of Wistar rats caused in them the signs of a general toxic effect which were manifested in the form of disorders of the liver function and of the morphological composition of the peripheral blood. With the indicated changes in the organism of the pregnant animals as a background, the harmful effect of VC was established on the development of the fetus and offspring. An embryotropic effect was detected just as in the microanatomical investigations of the offspring at various periods of postnatal development. Under the effect of vinyl chloride in concentrations on the 3 order of magnitude lower than the MAC (4.8 mg/m ) in the pregnant animals, according to all of the tests selected by us, no changes were found (compare table). Still, VC produced an embryotropic effect which took the form of permeability disorders of the vessels R&S 133824 7 of the fetus and of the functional deviations from the normal with respect to the nervous system, liver, morphology of the blood, changes in the weight coefficient of a few internal organs in the offspring. The experimental materials which were presented attest to the fact that concentrations of vinyl chloride at the level of those existing in the present MAC indicate a general toxic effect on the organism of pregnant animals and produce a pronouced embryotropic effect. Concentrations on the order of magnitude less than that of the MAC do not indicate a general harmful effect on the organism of pregnant rats, nevertheless they do cause a significant disruption in the embryogenesis which confirms the presence of a select effect of the toxin on the development of the fetus. The resulting materials indicate that we-should reexamine the MAC for vinyl chloride in the area of the work place with respect to the identified embryotropic effect. R&S 133825 LITERATURE Jlbi6an A. 11. H jp. -- Ap.x. 3HaT,, N* 10, c. 89. Allman Subarashan K. --"Develop. Psycho- biol., 1971, v, 4. p. 97--114. John 1. et al. -- Toxicol, appl. Pharmacol., 1977. v. 39. p. 497--513. --r vn --* y^ 30 fio CO CO _ ^ 0W0 n-x.--jfi ^ O) > :`l * y. ' . i TM:-t**.yryyr-:*'^ ;_yy tyyv`.y._w c t yV i *7 1.' .__' * ' . \ .*.'. Li'.' ^* \~V' :: , . . .- ---- --- --; -*, ^j ^-`- ^^~-=7 r - (c_ iC~^ /c./y'/Q /l)2),q J- f/l& 51/gs/tS c Wy^ Z Ccy^lw, V. C3J C*'lOfQ yZK 5U.iJ':) 17.< 13.133.: I-07:i I5^.014.45 .1. C. CA/lbHHKOBA, H. A. 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CozepHcaHae ra3a a 3axp330HKbix Kawepax co-CTaBZHZo 35,53,5 mt/m1 (ypOBCHb DAK) n 4,80,5 mt/m-1 (Ha nopzox minte FIAK) l. HatHHaa c 16-ro zhh 6epe:.ieHHocxa Kpwc -oSczezoaaza no pazy HHxerpazbHbtx h cneuit* (JiHMecKHX noKa3aTezefi. Azs BbiHazeHKH noapentzarauiero bzhhhhh BX Ha nzoz aacxb Kpbtc 3a63aZH Ha 20-h zehb flepexeHKOCTH. Bhibhczhzh npez- h nocxaunzaHTamioHHVjo, a TaKTKe oOmyra BMfipiioHazbHyzo cMepTHOCtb. flaxozorHio BHyxpeHHtix opraHOB nzozo3 H3yaazH na cepiiH epeaoa (.vexoz Wilson b MOZHtJxiHamiH A. n. Aw<5nHa it coaax.). B CBH3;t c xe.vt hxo nopoKH pa3Biix;ia tacxo nposi3ZfnoxcH a nocxHaxazbHOM nepnoze, nacxb Kpbic ocxaazHZH zo ecxecx3eHHUx pozoa. ripn 3X0 M )"HilXb;3aZlt K03li4>l<^HeHTb< BbI7tt3aeMOCXlt h zaK'auiiH. HaHHHa.a c 3-ZHeaHoro B03pacxa y noxoMCxaa ncc.xezo3azii BW3B3HHyso Z3i(raxe.-.bHym 3Kx;i3Hocxb, a c 2-necnHHoro B03pacxa -- peaKHHit nitmeBoro nosezeniiH. notca33TezHMH. xapaxxep;i3>'!otniiMH BU33aKHyro ZBitraxezbHVK) aK71I3HOC7b nOTOMCTSB KpblC paBHOTO 303p3CT3. c-xyiHiiZit apeMH HaxojHzemiH HtiiBOTHbtx Ha ropiUOKxazbfioit Bepeaxe. Bpe.'-ia Maxo^zenns itx Ha BepxnxazbHyx aepeBKax Tpex znaMexpoa (II, 17 h 25 mm), p33ZH<tHb;e 3ze.MeH7bt zsiiraTeZbKon peaxmiH hb BepiiiKazbaon Bepesne (Altman h Subarashan). Bcero npoBezeHO onozo 230 aHaziuOB, noKa3aTe-TH.Mi1 *niimesoxo noBezeHHH* czyiHiizif cnemtazii3npOBaHHb;e 4>opMbi annexuxa, ofimero annexitxa h jKaTKZbi. B 6-MeCfltHOM B03paCTe nOTOMCTBO OficzeZOBaZH (c ynexoM noza tkiibothwx) no pstzy noKaaaTezei'i, OTpaiKaramiix cbvHKUHH HepsHoii cncxeMhi, nenehii, noneK. inyHazH MOptpozorimeCKiiri cocxa3 nepiiipepitHecKon Kposn, yHitTbisazit KOSiptpnmieHTbl M3CCbl BHVTpeHHHX OprOHOB. Bcero b SKcnepHMeuxe 6btzo ncnozbaoBano 130 Oepe.MeHKbix xpbtc. nposezeK aHazita 230 hzozob. nozyneno 11 Haxozuzccb noz Ha6.:razeHttCM 700 ocofieu noToMCxaa. P13 y .u t 3 7 h it c c z e z 0 a a h it h. Pe3yzb-zTbi iiayHeHitH oCtnero TOKennecKoro zeu- 1 Oxpa0oTK3 pe:-K:iMa b BaTpaBotHbix xastepax npoBoznzacb B. H. rzyweHKO. 3a hto npimociiM rzySonyio fizarozapHocTb. cxBiiH BX npezcxaazeHbt 3 Taozinze. yexanoszeho. hto BX s KOHixeHxpamf.t Ha ypoBHe cymecxayrazaeft njlK Bbiabtaa.i y CepeneitHbix i+tnaorHbix yMenbateHite cozepaiaHita 3pnxpourf703 a Kpcan h CHitJKeni'.e cozep7K3H;ia rMnnypoaoi't KttC-70Xbt 3 MOHS, nplt B03ZeiicXBii;t HZT 3 KOKue.HTpamnt Ha nopazox Hitace OAK oxKzor:eH;tft no Bcest BbtSpaHHbt.M noKa3axe.THit no cpaaKennio c xoHTpozeM He bwhbzsho. BX b yKaaaHHbtx Bbime KOHtieHTpatutax He bzhhz OTpimaxe-7bHO Ha noKa3axe.7H npez-, noCTHMnZaHXaUHOHHO.'t H ofitiieil BltfipHOHBZbHOtt CMepxKoeTit. UcczezOBamte n-aozoa stitKpoa^-taToititHecxitM itexozoM noKaaazo yae-iHHeHne oxHocitrezbHoro HHC.ia hx c Kpa30ii3Z;:sH;tHMH (S1.2r;6.3 %; P<0.001 npH zei'icxaita Cozbtzeft KOHtteHxpamtii: 49,33.7 %; P<0.001 non zeii- cxBiiit Meuhtneit KOHueHTpaaaa: 12,3^3.2 ^ a KoHtpoze). npw B03zeitc7a:in BX b KOHUe.-txpa- UHit 35.5 jtr/m5 OT.MeneKO xaKTne yseznneHite HHCia nzozoB c oxenHOcTbio (62,0^7,9 npH 4,2i;3,2 b KOHTpoze; P<0,001). Bo3zeHCT3:te BX Ha fiepe:.ieHHbtx Kpbic b KOHueHTpaanax 35,3 h 4,3 mt/m' He npH30znzo k HBMeHeHHio xaKH.x noKazaTezei't, KaK Koyib^atineHT zaKxamni k KosiJicJmaiieHT 3bt:K;t3aeM0cxit noxoMcxaa. AH3ZH3 noBezeHHecKHx peaiantS y noxoMcxBa, nozaepraanterocH b aHxeHaxazbHbtH nepnoz B03ZeitCX31IK) BX B fiOZbUleiA KOHUeHTpaUHH, Bbthbhz yse-iitHeHite BpeMeHH yzepmitaaHitH Kpwchx 9--ll-ZHe3Horo B03pacTa Ha BepxHKazbHoit eepeane (93,38,49 c npH 23,83.83 c 3 kohTpoze; P<0.001). 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Tan, HanpitMep, y no* TO.MCTBa MyntcKoro noza ncpBoft onuTiton xpyn* nu OTMexeno CHttHceHHe opneHTitpoBoHi-oi'i peaKulnt (2.10,41 yc.T, ez. npit 4.1 0.5 yc-i. ez. a KoitTpoze: P<0,0l), COn (4.70.3 ycz, ez. npit 5.60.3I yc.i. ez. a KOHipoze: P<0.05), CHit:4cen;ie reMorzofimta b Kposn (13.15 r/z npn 1697,6 r/z a KOHxpoze; /><0,0I), CHnnce- Hiie KoacpipHuiteiiTOB Maccw cepzua, neHenn, noneK h cezeaeHKit. Y noxoMCTBa atcucKoro noza btoiI ate rpynnw Hafiziozazocb CHiiTKeHite cozepHtaHitH zenKotiitToa b kpobh, yBezHteHite kozh- necTaa rnnnypoaon khczotu a stone, yae-THne* Hite BpeMeHit renceHazoBoro cita, CHHiKenne Koadnpiu>HeH7a vtaccu cepzua. 46 . `-------- --- _ * ` ^ ' 7-*C - --C Pejy.ikTiTU ofic.iejoaaHHH Kpwc, noAsepraatiJHXCH bo Bpe.sia 6pe'ieHMOCTM sojAtfHCTBHt-') BX b kohueHTpauHSt 3.5,5 h 4,3 r/x5 rioKJ laTe.lb KOrtT70.:tK3^ (rt 11) r?yn.-;j 4.3 Mr. y* (n- t 01 4 35.3 vr ,'4I |nl9| Cnn, yc.i, eA. KopH03b;a pe^Aexc. yc.i, eAOpaeHTapoaoHKaa peaKaan, vca. eAPeKTa.ibHaa Te,MnepaTypa, 'C CHA, yc.T. ej. CoAepHtaHHe Hb. r/A BpHTpOUHTbl, 101: A TTeftKouiiTbr 10* a THnnypoBaa KacAOTa, Mr/.siA jlHVpe3, MA BeAOK a Mone, mt/a XAOpHAbl B Move, MMOAb .Macca reAa. r Ko3$4iHiiaeHT Mac.cw BHyrpeHHH.x op- rawoB; M03T cepaue nerxae ne^eHi* nOMKH ce-ie3eHK3 4,90,14 5,8^0.57 7.90.S6 36,4^:0.12 66.69,9 I255.4 4,90,15 12,8I .4 !45.2I5.0 5,70.76 5750760 0,050,004 3426.7 0,500,02 0,29^0.008 0,550.0I 4,70*7-0,2 0,54;0.01 0,400.02 4,1 0.12 7,51.54 11.2=1.03 36.80.09 53.5 12.9 1328,2 4.80.22 11,00.1 139,6=10.8 4.50.53 5590=1040 0.05=0.006 347=10.3 0.49=0.02 0,280.00G 0,56=0,01 4.97=0,25 0.57=0.01 0,41=0,03 4.7=0.1 7.6=0.92 10.2=1,04 36,7=0.1 84.6=12.4 128=5.2 3.9=0.33 P< 0,02 11,4=0.84 96.2-12,9 P< 0.05 6.7=0.55 4450=430 0.04=0.003 313=13,4 0,57=0.03 0.33=0.01 0.62=0.03 5.06=0.12 0.60=0.02 0.51=0.05 npw ofic-iejosaHHii noTOMCTaa BTopoii onwir- hok rpynnu (4,8 Mr/M1) TaKXte awflaneH ue.ibia P*U H3MeeHtifi. y ca.MU03 otmchcho CHHiKeHae reMor.iofiHHa (I468 r/A npa 169=7.6 r/.i a KOHTpo.ie; 0,05) h cojep^aHHS achkouhtob a KpoBH. yae-isiHemte coAepxaHaa ninnypoBofi Kiic.ioTU a Moae (141 14,3 Mr/MA npn 88,3= 9,4 vt/sm a Kompone; P<0,01), HJMeKeHite X0jCp(pntL!(eHToa Maccw rteaeKa, noseK, ceAeaea- kk. y caMOK 3Tofi rpynnu HaSAioAaAocb CHH>Ke- Hite cojep^aHiia apHTpomuoa h AefiKOUHTOB b Kposa, yae.ianeHne coAeparaHaa rannypoaoK khc- aotbi a wone it cwiHJeHae KoxfxJjauaeHToa .vac- cm ,ierKHX a neaeHa. 3 a k .1 ra a e h a e. BX a KoaueHTpauaa Ha ypoaHe cymeCTsyiomea rij3K (35,3 mr/.\<5) npH BoaAeacTana b Teaenae Bcefi 6epei<eHH0C7H Kpwc Baciap awauaaa y mix npa3aaKa odtuero tokcaaecKoro AefiCTBaa, KOTopwe BbipajKaAHCb b HapyuieHHHx (pyHKuaa neseHH a MOp^ononmecKoro cocraBa nepKcpepitieOioii KpoBii. Ha tpone ynaaaHHbix H3MeHeHaft b opraHH3:.ie fiepeMfiHHbix hthbothux 6wao bwhb-icho nospeHCUforuee bahhHae BX Ha p33BaTae n.ioAa h noTOMCiaa. 3mfipaoTponabia aiJxpeKT CbiA ofiHapyHien Has npa MHKpoaHatOMHHecKOM HccaeaoBaHmt noiovcTBa b pa3nwe nepaoju nocTHaTaabaoro paaaaTaa. npa 303AefiCTaaa BiiHHAXAopHAa b kohuchtpauaa Ha nopHAOK mince flAK (4,8 r/M3) y (Sepe.MeHHbix HrasoTHbix no Bce\i BbiCpahhhim Ha- xa TecTa.M lOMeaeimft He HaHAeao (cm. xaS.nH- uy). B to nee Bpexfl BX jasa.i SMfipaoTponHbifi aipdieKT, KOTOpbia npoHBAsuca a Hapyuienaa npo-, HauaeMocTH cocyAOa y n.ioao a cpyHKUHOKaAbHbix OTKAOHemiii ot HOpMW Hep3H0a caCTe.Mu, tieHeHH, MOp$OAorHH Kposa, H3.yeHeHaa kob^xJmuaeHTOB jiaccbi HeKoxopbix BHyTpeaaiix opra- hob y notOMCTBa. ripejcTas.TeHmre 3Kcnepii.He:iTa.ibHbie .vaie- paa.ibi cBaaeTe.ibCTByioT, hto HOHAempaunH BX Ha ypoBHe cywecTsyiomea b Hactomnee ape\tfl njIK 0Ka3WBaioT oduree TocxanecKoe jeacTsae Ha opraHa3M fiepeneaHyx hcabothwx a aaioT BbipaHieHHbia 3MfipaoTponHbiS arpcfieKT. KohuchTpauaa Ha nopHAOK mince nUK He oKaawaatOT odwero noBpencAaioiaero Aeftc73HH Ha opraHiia.M fiepe.MewHwx Kpbic, ie,M He yeiiee awabiaaioT 3HaHHTe.ibHbie Hapyuie.Han a aittfpaoreaeae, mo- ' noaTBep.HjaeT Ha-Timae aafiKpaTe.ibHoro b-thh* hhh HAa Ha pa3BHTae naoaa. no.TvaeHHbie MaTepaa.Tbi yKa3biaafor Ha aeofixoJHMocTb nepec- MOTpa njK BX a B03ayxe paConea 30hw c yqeTOM BhlHB.'.eHHOrO 3M6pllOTpOr.HOrO AeliCTBHH. TIHTEPATyPA Ubi6an A. n. a Ap. -- Apx. aH3T., .V 10. c. 89. Altman Subarashan K. --"Develop. Psvcho* bio!.. 1971. v. 4, p. 97--114.' John /. et al. --Toxicol, appt. Pharmacol., 1977. v. 39, p. 497-513. nocrynH.ia a pcjjkuhkj 19/U 10T9 r. * I i. ,1 I'' R&S 133827 XOPOLUrl.lOBA H. B.. CA/IbHUXOBA Jl. C,, AOMLU/IAK M. V,, HHPKOBA E. M. Maynemie wy-areHHOro x rcHa,ioTpor.Koro .cei'cvana niypaMa 30 H-lbHUUKAJI A. B,, KA/IHHA O. B. UHPKOBA H. Jl.. wabo.hiha t. a., op103A T. B. riinieHimecxan oueuxa hobscx ycxaMCaox a-tH nnaaueHHOiV peaxn THMOQHEBCKAR Jl. A, HBAHOBA H. IL. BAJffclh'HHA E. C. ToKCH.xo.rorna admpoa o-4>T3.re3oii icxe.ioTbi h hx rHr;teHHwecxaa pernaMeHTauna 20 22 25 KHOP.OSUILOVA. N. V.. SALNl-'OVA. L. S.. D0M3HLAK. M. G.. -CHIRXO'^A. E. M. Alularjenlw and gonadotropic a:::vitiei of tfii'j.-am EF ILNtTSKAYA. A. V.. KALIN'A, ' 0 TSIRKOVA. N. L.. SHABOLIN'A. T. A.. ORLOVA, T, V. Hygienic evaluation of ne`.v devices for plasma cutting TIMOFIEVSKAYA, L. A.. IVANOVA, N. 1. BALYNINA. E. S. Toxicology of esters oi O-phthalic acid and hygienic standards for; them Kpaixne cooCmeHHfl 3AFIUEBA H. H. Onut 03iopoBneHiiH yc.io- bhh Tpy^a pafiaKHX. conpHKacasounixca c pTyTbio, na 3o-iOTOodoraTHTe.tbHt:x <}>a6piixax BACH/IbEBA E. B., 0P/10BA A. A.. 03EPOBA B. B., CMHPHOBA M. H. K-v.ihhkodiyHKUHOHa.ibHoe H3yMeHi(e asTine-i k Hao.- noHeHHHKSM h uiHTOBiiaHQH sceneae npi< neKoropwx npotjjeccHOHa.ibHWX aaOo.aeaaHHHx -lerKHX BPOilCKHFl O. B. 3aeKTpopeHTreHorpat|mH npx nHCBMOKOHHoae AF13HHA H.'/I. O rywopansHoff ayroiiM.MVH- ho it peaKttHH npn npotjjeccHOHa.ibHOM nuneBOM ipOHXHTe BOBbiKHH C. C., AHAHbHHA T. B.. A5PAMQB LU. H, CocTOHHite cfjyHKUHH annapaTa BHatuHero auxanufl y pafrmx CH.iHKaxo- h raaociUHKaTHoro npoHaBojcxBa AHHCWMOBA H. H. B/IArOHAPHAS 0. A., KPHMKOBCKHFI E. A., CA- . XAPOBA /I. H,, HHKHTEHKO T. K. TokcitxoaorHHecxaH xapaKxepHCTHKa (pxopoxaHa . CAPy.XAHSH XC r,, BABAflH 3. A,, HA- 3APETSH P. A., OPAHECSH P. HAP- B1IHSH T. B., KAOSH B. B. O bosmowho- Ct;i .IBHeHitH y HHTHOaOM H WexHOKHHOM xpo- HHHficxoii MO.iii6aeHoaOH HHXOKcuKauwi y fie.xyx xpuc 30YI0THHK0BA T. n. 0 Hapyutennn it.MwyHCUorittecKoit peatcxi'sHocxH opraHiia.va noa BoaaeftCTBHe.M necTHUitaoa b yc-iOBitnx xen- THU HTHATfOK A. H,, KAPflOB B. BE- PHH B. K. H3yiteHf:e aeMCTBits aapoaoneii c.MaaoHHo-oxaa^aaioutHx tKiuKOcxeft Ha op- raHH3.\( a aKcnepHMeHxe HBAHOB H. I\, MEJIbHHKOBA Jl. B., K.TSHKHHA A. .M.. TEPMAHOBA A. Jl. HccaeaosaHHe xokchhhocxh, onacHoent it xapaxxepa Bpeanoro jeficxaiia na opraHi(3M r.xiiminona rHE3nH/10BA A. H., BACH/IEHKO H. M.. MAH/KEJIEFI E. C. SiccnepHMeHxa.xbKae o<5ocHoaaniie IIUK nbi.m anexH.ica.XKmtnoaofi km emu a ao3ayxe pafioneit 3ohw 28 29 31 33 34 "36 38 38 40 42 43 OASEE3 A. H. AlaxepHa.iu no odacHOoa* Hino DAK apceHiiaa ran/wa a aoaayxe pa- fio'tet't 30Hbi 4 Brief Communications ZAITSEVA, N. N. Experience with impro vement of working conditions for workers exposed to mercury in a gold-dressing fac tory VASILIEVA. E. V., ORLOVA, A. A.. OZE ROVA. V. V, SMIRNOVA. M. I. Clinical 'functional study of antibodies to adrenal and thyroid glands in some occupational diseases of the lungs BRODSKY, 0. B. Electroroentgenography in pneumoconiosis AIZINA. S. L. On the humoral autoimmune response in occupalior.al dust bronchitis BOBYKIN, S. S,, ANANYINA, G. V,, and ABRAMOV, Sh. I. Respiratory function in workers of the silicate and gas-silicate industry" ANISIMOVA. I. G.. BLAGCDARNAYA. O. A., KRECHKOVSKY. E. A.. SAKHARO VA. L. N., NIKITENKO, T. K. Toxicologic characterization of fluothar.e SARUKHANYAN. Zh. G,, BABAYAN. E. A..' NAZARETYAN, R. A.. OGANESYAN. R. D,, DARBINYAN. G. V.. KAFYAN. V. B. On the possibility of treatment by unithiol and methionine of . chronic molybdenum poisoning in white rats ZOLOTNIKOVA. G. P. On the impairment of immunologic .reactivity caused by pesti cides used in hothouses IGNAT1UK, A. N., KARPOV. B. D,, VERIN, V. K. Experimental study into effects of aerosols of lubricating and cooling liquids IVANOV. N. G.. MELNIKOVA. L. V., KLIACHKINA. A. M,, GERMANOVA, A. L. Toxicity, hazards, and harmful effects of gtycidol - - GNEZDILOVA. A. t,, VASILENKO. N. M.. MANZHELE1, E. S. Experimental determina tion of MAC for the dust of acetylsaHcylic acid in the air of work areas FADEEV, A. t. Materials for use in setting a MAC for gallium arsenide in,the air of work areas CA-lbHHKOBA .T. C., KHU.OBCKAS II. A. B.tHSHHS BHHH.lX.lOpHaa H3 3w6pHOrHe3 Kpuc 46 SALNIKOVA. L S., KITSOVSKAYA. I. A. Effect of vinyl chloride on embryogenesis in the rat - - * ' ` - SO r &S 133829