Document mpB07j3armzm9oR8vbDyLkErZ

AR 226 _ 0784 ACUTE TOXICITY TO INVERTEBRATES (GRASS SHRIMP) IEST~ S~ U ~ BSTAN 0000C 0000E 0000 Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-206 or L- 3243. (1-Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was not noted. The test sample is FC-206, identified by the laboratory as "Sample C". Current information indicates it is a mixtureof 0.67% PFOS, 17.5% diethylene glycol butyl ether, 78.91% water, 1.33% Sultone foamer, 1% sodium octyl sulfate, 0.04% sodium lauryl sulfate, 0.5% polyoxyethylene monooctylphenyl ether, and 0.05% benzotriazole. - The following summary applies to a mixture with incompletely characterized concentrations of impurities. Data may not accurately reflect toxicity of the fluorochemical component of the test sample. M0 eTHOD: 0000 Method: Standard Methods (APHA) 1971 Edition Type: Acute static GLP: No Year completed: 1974 Species: Palasmonetes vulgaris Supplier: Field collected locally in MA. Analytical monitoring: Temperature, and dissolved oxygen. Exposure period: 96-hours Statistical methods: TLso (median tolerance limit) values calculated using a linear regression equation. Test organism age: Juveniles. Length and weight: Average length = 30 mm Loading: Not given. - Pretreatment: None Test conditions: Dilution water: Synthetic sea water Dilution water chemistry: pH Salinity 8.0 25 ppt Lighting: Not given. Stock and test solution preparation: Not given. Concentrations dosing rate: Once Stabilityofthe test chemical solutions: Not noted Exposure vessels: 5 gallon glass vessels containing 15 L of test solution. Num ofrb eplie catr es: 1 00147 Number of organisms per replicate: 10 Numberof concentrations: six plus a blank control Water chemistry during the study: Temperature range (0-96 hours): 20 + 1C Dissolved oxygen range (0-96 hours): 4.2 - 8.6 mg/L R0 ESOTS000000 Nominal concentrations: mg/L Blank control, 100, 180, 240, 320, 420, and 1000 Element values: 96-hour TLsy = 280 (230 -- 342) mg/L 96-hour NOEL = 180 mg/L Element values based on nominal concentrations. RSuebmsatraknsc:e TReesmtianrgkwsafsielcd.onTduhcetveadlouenstrheepmoirxtteudraepapslydetosctrhiabtemdixitn utrhee aTnedstnot the fluorochemical proportion alone. Conclusions 000000 The test sample 96-hour LCs for grass shrimp was determined to be 280 mglL with a 95% confidence interval of 230 to 342 mg/L. The 96-hour no observed effect level (NOEL) was 180 mgiL. Submitter: 3M Paul, Minnesota, Company, 55133 Environmental Laboratory, P.O. Box 33331, St. bataQuayry 000 Reliability: Kiimisch ranking 2. Testing meets the criteria for quality testing. However, the sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. - REFERENCES 000 Test was conducted by Bionomics, EG&G, requestofthe 3M Company, St. Paul, MN, Inc., of 1974. Wareham, MAat the OTHER _-- Last changed: 6/28/00 0014850 goessa ty Col Dogon bus D So"mhle codeadahol Stasi bow 95% BIOASSAY REPORT SUBMITTED TO 3 M company onbduio lems SAINT PAUL, MINNESOTA Cpr) obtand? | Med bv crobaw 7) be ] Pt donk en -- Cig ow? | i I ] ACUTE TOXICITY OF SAMPLE C TO MUMMICHOG (Fundulus heteroelitus), | GRASS SHRIMP (Palaemonetes vulgaris), AND FIDDLER CRAB (Uca pugilator). i J BEiGon&oGmi,cs Inc. !| W7a9r0ehMaami,n MSatsrseaetchusetts May, 1974 1 001481 METHODS AND MATERIALS ) These investigations were performed at the aquatic toxicology laboratory of Bionomics, E G & G, Inc., Wareham, Massachusetts. The susceptibility of mummichog (Fundulus heteroclitus), grass shrimp (Palaemonetes vulgaris), and fiddler crab (Uca pugilator) to Sample ! C. a brown liquid, tested as 100% active, was evaluated | under static bioassay conditions for a 96 hourperiod. The results were reported as themediantolerance | limit (TLs)), the concentration of the sample in water causing 50 percent mortality after 24 and 96 hours. The predictedTLsq value and its 95% confidence intervals were arrived at by converting the concentrations D tested and the corresponding observed percent mortalities. to logs and probits, respectively. These values were then used to calculate a linear regression equation. | Test procedures for the static bioassays are those described for Fish Bioassay Procedures in the 1971 | edition of Standard Methods (APHA). The mummichog, > grass shrimp, and fiddler crabs used in these tests . were collected locally by laboratory personnel. The mummichog had a mean weight and length of 1.1 g and 35 mm,respectively. The grass shrimp had a mean length of 30 mm. The fiddler crabs had a me Apace ==? 5 width of 20 mm. 001482 i . Lo So 3 . } | 1 ) | | I J | | 1 Page two The test species were observed in the laboratory hatchery facility for at least 30 days prior to testing. These species were acclimated over a 10 day period,prior to testing,to laboratory test diluent water quality and temperature. During that period mortality in the test population was<3% and these animals were judged to be in excellent physical condition. The bioassays were conducted in 5 gallon glass vessels containing 15 1 of water at 20C (* 1.0), and there was a single introduction of the sample into each test vessel. The test containers were not aerated. The test diluent consisted of synthetic sea water! with a salinity of 25 /0o0, a pH of 8,0 and a temperature of 20 (* 1.0). Dissolved oxygen levels for the tests ranged from 8.6 at the beginning of the tests to 4.2 at the end of testing. Ten specimens were introduced into each test vessel 24 hours prior to the start of I RESULTS The predicted TLg, values and 95% confidence intervals | are presented in Table 1. Table 2 presents a summary ~~ J Laroche, G. E. Eisler, and C. R. Tarzwell, 1970. Bioassay Procedures for Oil and Oil Dispersant Toxicity Evaluations. | J. W. Poll. Cont. Fed. 42 (11): 1982-1989. ] 001483 SE Page three 3 of observed mortality for each individual concentration after 24 and 96 hours of exposure. The mortality syndrome in all tests was similar. The mummichog and grass shrimp generally became dark and lethargic, lost equilibrium, and expired. The fiddler crabs became lethargic before expiring. ' 001484 PE | 3 SUBMITTED BY: PREPARED BY: APPROVED BY: i |i | | EBiGon&oGmi,cs Inc. W7a9r0ehMaami,n MSatsrseaecthusetts May, 1974 Robert E. Bentley Aquatic Biologist 7 Bevier Hasbrouck Sleight, III 2 Director, Freshwatr Programs -- i) 001485 Table 1 -- Acute toxicity of Sample C to mummichog (Fundulus I heteroclitus), grass shrinp (Palaemonetes vulgaris), a fiddler crab (Uca pugilator). These data are based on results of bioassays conducted at the fish toxicology of Bionomics, E G & G, Inc., Wareham, Massachusetts. | | | C. ond | imu. Species `mumnichog, grass shrimp fiddler crab : TLsg - milligram/liter Zh Reur 96- hour >1,800 <2,400 1,820(1,360-2,420 1,000.0 >10,000 (2o8e0(230-342) 3,260(2,760-3,860) No Effect (mLge/v1e)l "1,400 180 Le 2,400 ) i! ii I | Assays conducted at 20C ( 1.0), mean weight of the mummichog 1. Phssays conducted at 20C ( 1.0), mean length of the grass shrimp 30 mm. CAssays conducted at 20C (} 1.0), mean carapace width'of the idd crabs 20 m. 95% confidence intervals. - ) a todd wb spiked bh Bd olla? an J 001486 Table 2 -- Concentrations tested and corresponding observed ) percent mortalities of mummichog (Fundulus heteroclitus), grass shrimp (Palaemonetes vulgaris), and fiddler crab (Uca pugilator) after 24 and 96 hours of exposure to Sample C. ` Species | -_-- `mummichog ! ) Concentration (mg/1) 3,200 x 2,400 1,800 : 1,400 1,000 control % mortality observed BE 3 Tra 100 100 100 100 0 30 0 0 0 0 0 0 grass shrimp | | | ) 1,000 420 320 240 180 140 control 0 100 0 100 0 100 0 40 0 20 -- 0 0 0 0 J 001487 3 Table 2 -- Continued . -_-- E Species Co(nmcge/n1t)ratiN on Zh%omourtality oeobesperyveeld fiddler crab 10,000 100 100 6,500 0 100 : - 4,900 0 100 - 3,700 = 0 70 2,800 0 30 2,400 0 0 2,100 0 0 control 0 0 --- J 001488