Document mp8LJGpg47p8rMLeaK8G2ommk

AR R26 -- 0752 ACTIVATED SLUDGE RESPIRATION INHIBITION TEST TEST SUBSTANCE Identity: ArefmeirxrteudretocaonstaPiFnOiSng, pFeCr-f9i5u,oroorocatsanaescuolfmopnaotne,ewnohtficFhC-m2a0y3CaEl.so b(1eOctanesulfonic acid) (CAS # 2795-39-3). TRheematrekstss:ampTlheeis3FC-p2ro0d3uCcEt.ionCulrotrennutmibnefrorwmaatsio5n19i.ndicates it is a mixture of S1u.l8t4o%nePfFoOaSm,er3,03%.1d%iestohydlieunme ogcltyyclolsubluftaytle,et2h.e1r,%6p0o.l2yo3x%yweattheyrl,en2e.56% monooctylphenyl ether, 0.12% sodium lauryl sufate, and 0.05% tolyitriazole. cThhaerafcotlelorwiiznegdscounmcmeantrryataipopnlsioefsitmopuarmiitxiteusr.e Dwaittha imnacyomnpoltetaeclcyurately reflect the toxicityofthe fluorochemical componentofthe test sample. METHOD Method: OECD 209 YGeLaPr:CNomopleted: 1989 ASntaatliysttiiccaall mmoentihtoodrsi:ng:ResDuilstssolwveerdeodxeytgeernmicnoencdebntyrcaatlicounlsa,tipoHno,fatnhdete%mp. Tinehsitbitoirognainn irsesmpisroautirocne:rateA.ctivated sludge mixed liquor collected from the TMeetsrtocWonadsitteioTnrse:atment Plant, St. Paul, MN SDiylnutthieotniwcasteerw:agMei:llippeorreOMEilClDi-mQeTMtwhaotder Reference andtest solution preparation: A stock solutionofthe 5re0f0ermegnciens1u0bsmtLan1ceN,N3a,5O-Hd,icdhillourtoepdhteono3l0, wmaLswiptrhepMairleidpobrye dMiislslol-vQiTMng awantderd,iltuhteend tboro1uLghwittthotMhielip-oQinTMtowafteirnc.ipTihenetpprHeocfiptihtaetiroenfweirtehnc1eNsoHlzutSiOoxn was measured to be 7.8. Test substance was added directlyto test vessels. TNeusmtbveerssoeflsc:oncNeonttgriavteino.ns: 5 plus 1 reference substance and 2 Tbleamnpkecroantturroles: 19-21 C Total suspended solids and pH on dayoftesting: 4.1g/L (TSS), = initial pH Element 7.2 and Basis: fRiensalpiprHati7o.n5 inhibition as determined by oxygen `consumption. 001107 Remarks: Values corrected to 20 C for calculations. RESULTS Nominal concentrations: 2 Blank controls, reference substance, 100, 180, 320, 560, and 1000 mg/L. Element values: 33-0h-omuirnuEtCeeEgC=so>1=0>0100m0g0/Lm.g/L Element values based on nominal concentrations. Remarks: Substance RTeesmtairnkgswfaiesldc. onTdhuectveadluoens arempioxrttuerdeaapspldyestcortihbaetdmiinxttuhreeTaensdt not the fluorachemical proportion alone. conclusions The test substance 3-hour ECso for activated sludge respiration inhibition was determined to be >1000 mg/L. PSauublm,itMtienrn:esot3aM, C5o5m1p3a3ny, Environmental Laboratory, P.O. Box 33331, St. DATA QUALITY. RHeolwieavbielri,tys:amKpiilmeispcurhitryanwkaisngn2o.t Tpreospteirnlgymceheartasctalelrcirzietderiaanfdorthqeuasltituydytelsatcinkgs. analytical confirmationofthe amountoffluorochemical proportion in the solution. REFERENCES TPhauils,sMtuNd,y LwaabsRceoqnudeuscttendumbbyetrheG133M58C-o1m,pa1n98y5,.Environmental Laboratory, St ome... Last changed: 6/26/00 001108 . ACTEINVVAITREODNMESNLUTDAGLELMAIBXOERDATOLR1YQUOR wGR 1358-) bate o=9-29 Analyst SUR pe reosedAovivatel Sloe Recpivation Tobi lion #209 souzeeMoscaLacie TeeatrantPlant GePol,pwr Dace Obtatned_p-9-29 Date Uses__6-Q~9 Mixed Liquor Suspended Solids (MLSS) 4.1 8/L Adjustment None g/L PH noted = 72.2 Comments, Etnal > 7.5 v0 _ Test Substance sample Descripeion FC - 20305 [ot 579 - Sample Prighess Core,lowopalyz220asucnantNg eee Comments_ Bference Substance ssaammpplleedpeLscSritgpketoSnDo3ln,i=5c7.h 2 loAdsrustouenpt_Ah oneonaf Comant- ExposureConditions Test Duratton___ 3adHours - Test Temperature 19-2 c comence_ Ran pith LRC1357-2 ------ -_-- -_-- 001109 rams 0.00 LabRequest No.G[358-| `SampleDescription FC-203t Lorsiq Environmental Lab -- WorkSheet RESPOIREACTDIAOCNTIINVHAITBEIDTISOLNUTDEGSET #200 200 --Lof 3 oat: o-9-8F Analyst _Sedfa, _ tessa Prepaarion FEST _------ -- \ Test Conc. mgadded ml DI water ml syn. sewage mlmicrobial Total volume Iie loo = gq + lo So + 284 + Ib + Roo = Sco + Roo = Soo [80 90 + 2g4d + le +r Reoo = Soo 320 160 + gq 4 b+ Roo = Soo SLo 280 + 284 + lo + Roo = Seco Jooo S00 + RY + lb + Foo = Son --_---- eee------------------------------------ -_-- _-- -_-- _-- -_ _-- -_-- -_-- --_---------- 001110 WwGI358-1 . ENVIRONMENTAL LABORATORY - OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209 2 RAW DATA ra 43 Test substance: __EC = A03C Lot 5/9 Source of Activated Sludge Mixed Liquor: MetroLassT e ieat Plai nt Sntteadnst d Date eg ed - = Date Used: L-9 8 Mayet; BEST COPY AVAILABLE 30 - MINUTE EXPOSURE DISSOLVED OXYGEN CONCENTRATIONS 20 Conce[ensttratSiubosnta(ncseg) HME-NINU eTEM conteoL 1 [Z.gle[z0leslleu.s]ovsalsslslss0olae Ep20 20 CONTROL 11 ee [72703575044137[3.1]2.51191/5 lao [easels [20[3702 2a slogldad lac 2 Hi I 3 - HOUR EXPOSURE DISSOLVED OXYGE! CONCENTRATIONS po Conc[eenTtratSuibosnta(ncneg/L) WE-RINUTES TdH como 1 7.8 re[zo slolsaldal4133]3132q0 ka oo |zilegdrlrh3allzzlssadlalzlasa le 20 le.q]szlpa(8.1]j72d7.[73]eq 1571509 a0 [onlss82]78kb 7]le7.3l45q]lSsnls1 Ig vo Jed1gzlzaglezdleOl[sz05aallns2 lo Plt of Activated Sludge Mixed Liquor: TPeHstofThemipgehreastturceo:nce|nqt-ra&ti|onC tested: Initial 7-3 Initial 7,5 Final 25 Final 7.0 001111 - ENVIROMENTALLABORATORY ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209 : (CALCULATION OF RESULTS rest substance JC=A0B0% Lod 4/9 rE po ys Pa 343 30 - MINUTE EXPOSURE oncentration (ng/L DNTRQ 0 20 o 560 v0 ONTRO R fo BC Respiration Rate?) |_o.5p55 |osose | = | 0.5322 |0.5329 | = |0.4|3 oe3 zaz31 - = | 0.9242 |o.924g | = |08137 logs ~[ - rr 0.5345 [osi0s | - vercent Difference in Controls() Cg (95% Confidence Linits) mg/L 0% ma lL QR BOSE ConTceesnttrSautbisotnan(cneg/L) QT 0 120 20 LO Oo; CONTR) Oxygen UpRtake Rate RtoCo2r0rCected + 0.5076 |05/27 [0.527 | 0.2904 |o4ds0 1 | 0.3200| 0.3479 |_.2958 |o.3952 | | 0.5337 |o.5032 | rr 1 [o | os|5 o05 %3091 IRnehsipbiirtaitoinoniRnate (oy = 20 2 i A 2) ~-- percent Difference in Controls) 4.5 % . gg (854 Confidence Linits) pe mg IL (1) Teemmpp., Corrections. o (ngm8 0,Oy/L-min.) (3) Difference= Ee +--= dx 100 : fRC,e+ R)C. x (2) % InhibitionT fic) X 100 7 RTea=boso+OxSygaennpietatkeemrpa,trateeC(omg C O,/-Lm-imni.n.) 2 L 3s 1.047, x = T,obs - 20C = Control The tuo controlrespiration rates should be within 15% of each other See pad for test Soin. . prep. . 001112 eiriame ample Description ,5- Dichlorophenol eLerence. Substance Add Zeal Label ot D7- 00 -o Environmental Lab -- Work Sheet OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209 Stock and Test Solutions Preparation gregr Lab Request No. 1 358) "0 --L-- of 2__ ate: =l-o 9-29 Analyst _Sabfd STOCK SOLUTION: i oma . "Diluted to 3,5-DeP [lo 5 Bol with Milli-Q wale =0.S Mme "Diluted do 10 Lider wih Mill-Q water Stock Solution ph= 7.9 -- e-- ee ---------------------- ostConc. mlstocksoln mlDI water mlsyn.sewage Wlmicrobial Totalvolume ng/L REL, S500 mL Ber 500 ml feedRor, 500 ml inoculumRSL, S00 m1 nl pnifols = A8Y + 6 + Zoo = soo /o lo + 274 + lb + zoo - Sco ee r e-------- i ------ ------ ------ ---- -- Control rek Ww LR 6 13259-) mee ----------eee rerereremeeeeseero e--e ------------------------------ Sm S-- r --e ----e-- er--------------r~r-------- -- e--e ---------------------------- ------rm -- -------- -- ------e -------- -- r-- e -- ----r-- ------s-- eS --------------ee-- e ---- reesei----------------reremo Somme eterna a eee GOR448 " G61r 357i2 ce - ENVIRONMENTAL LABORATORY : OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209 rR $3 RAW DATA Test sustance:_3,5~ DNIChlorophengl Referpne Sobsiame Source of Activated Sludge Mixed Date Received: (o-9 2 Uquor: MetroWaste,Teeetnnt ClantSP Date Used: _6-7-89 aw mise Loe 68 Fo, BEST COPY AVAILABLE 30 - MINUTE EXPOSURE ConcaESnTtratSiubosnta(necge/1) DISSOLVED OXYGERNE-HICNOGNCTEYNSTRATIONS go o&R contro 1 22 7e70[e.0en rsl[1s 03c53r.0[oa l20l7.a 2623070[7].9eol57154]5) 2)| CLTTTTTTTTT1 L CT r TT r TT r 1T 7 CL TT rT1 pr CLT TT rT 727.0 [us5al5 [4a[4338 53 lo 7lea lm] 3 HOUR EXPOSURE ConceESnTtratSiubosnta(ncaey) DISSOLVED OXYGEFNE-CNOTNRCUETNETSRATIONS ma D 4.3 19519. [90(s.9[96]a5]83]g 8 CTTT TTTTTT1] NE EN IA CLT CETETA TTTTT I I CONTROL 11 [r3l7leeleols4 914332Balan ol] pBHootTAacttdinveasttecsonScleundtgreatMiioxnedteLsitqeudo:r: IInniittiiaall 70 . 2 FFiinnaall 77.29.5 001114 . ENVIRONMENTAL LABORATORY IR lsalr . ACTIVATED SLUDGECRAELSCPUILRAATTIIOONNOFINRHEISBUILTTISON TEST #209 ADnaatleyst9S.r0y9 Test Sibstance T 35o=- I ichloKmodepronhee Soubsnbtsatansncee| 3 3.43 30 - MINUTE EXPOSURE ConTceestntSruba:tion. (ng/L CONTROL 0 Ooveen Uptake Rate Loge (1) * fesasbhrieeidton $ Rare( |o Soss 1 on Soos | = [| 0.3145 |o.3004 | 4] 1 rr CONTRO) 0.5105 = Percent Difference in Contro(ls) % ECgy (95% Confidence Limits) mg/L LO male. SiR EXpoar e { Zon ConTceesnttrSautbisotnan(cneg/L) CONTRO 10 CONTRO OxygenESUptake Rate RtoCo2r0reCcted + RIenshpiibriattiioonniRnate(?) . 53/5 -- | 0.7655 loigd 1 rr ; 1 rr 1 rr 1 1] : = Percent Difference in Controts()__ 4.5 % Coy (95% Confidence Limits) ng/LLO male induc :. in ) Tew, Corrections oLBS Re, + RC. (@ Inhibitions: 72). (ag 0,/L-nin.) Xx 100 (5) +Differences( L--+--=Z)x 100 Tshheoultdwobecownittrohlinr1e5s%piorfateiaocnhroattehser here: z See pa Adertest S T,o=bsOl xy&geneuptatkemra:te(nvgeb0,/L-nin.B) EST COPY Soln. AVAILABLE . Or P' . C = Control 001115