Document mp2Zq3GadLG4gdBYkjkwv2oOB
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ACCUMULATION OF PERFLUOROOCTANESULFONATE AND RELATED FLUOROCHEMICALS IN FISH TISSUES
John P. Giesy* Kurunthachalam Kannan Department o f Zoology, National Food Safety and Toxicology Center, Institute for Environmental Toxicology, Michigan State University, East Lansing, M I 48824, USA
*To whom correspondence should be addressed: Prof. J.P. Giesy
218C National Food Safety and Toxicology Center Michigan State University East Lansing, MI 48824 USA
Tel: (517)-353-2000; Fax: 517-432-2310; E-mail: igiesv@aol.com
Prepared for: 3M St. Paul, MN June 20, 2001
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SUMMARY
This report is a compilation of results of a monitoring survey of perfluorooctanesulfonate (PFOS), perfluorooctanesulfonamide (FOSA), perfluorooctanoic acid (PFOA) and perfluorohexanesulfonate (PFHS) in fish tissues from the US and certain European countries. The greatest concentration of PFOS found in fish was 923 ng/g, wet wt, in the muscle of fish from Scheldtz River Estuary in Belgium. The highest concentration of PFOS in Great Lakes fish was 297 ng/g, wet wt, in the muscle of carp from Saginaw bay, Michigan. Muscle tissues of fish from several inland lakes of Michigan did not contain detectable concentrations of PFOS. Similarly, fish from the North Pacific Ocean and Antarctic fish did not contain any of the fluorochemicals analyzed. FOSA, PFOA and PFHS were rarely found in fish.
INTRODUCTION
This report is a compilation of results of a monitoring survey of perfluorooctanesulfonate (PFOS), perfluorooctanesulfonamide (FOSA), perfluorooctanoic acid (PFOA) and perfluorohexanesulfonate (PFHS) in fish tissues from the US and certain European countries.
MATERIALS AND METHODS
Fish tissues were collected from various locations (Table 1 for details) and stored at -20C until analysis.
Analysis Details of the analytical procedures have been outlined in standard operating procedures for the analysis of fluorochemicals in tissues which are stated below. Preparatory Methods. ETS-8-6.0, "Extraction of Potassium Perfluorooctanesulfonate or other Fluorochemical Compounds from Liver for Analysis using HPLCElectrospray/Mass Spectrometry" . 3M Environmental Laboratory, St. Paul, MN.The analytical wok was generated by the 3M Environmental Laboratory. (Refer to Reference (1) for the specific reports.)
An ion-pairing reagent was added to the sample and the analyte ion pair was partitioned into methyl- tert-butyl ether (MtBE). The extract was transferred to a centrifuge tube and put onto a nitrogen evaporator until dry. Each extract was reconstituted in 1.0 mL of methanol, and then filtered through a 3 cc plastic syringe attached to a 0.2 pm nylon filter into glass autovials.
Analytical Methods. ETS-8-7.0, "Analysis of Potassium Perfluorooctanesulfonate or other Fluorochemicals in Liver Extracts Using HPLC-Electrospray/Mass Spectrometry". 3M Environmental Laboratory, St. Paul, MN.
The analyses were performed using HPLC-ES/MS/MS by monitoring a single product
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ion selected from a primary ion characteristic of a particular fluorochemical. For example, molecular ion 499, selected as the primary ion for PFOS (C8F17SO3-) analysis, was fragmented further to produce ion 99 (FSO3-). The characteristic product ion 99 was monitored for quantitative analysis.
Analytical Equipment The following are representative of the actual settings used during the analytical phase of this study.
Liquid Chromatograph: Hewlett-Packard Series 1100 Liquid Chromatograph system Analytical column: Keystone BetasilTM C 18 2x50 mm (5 pm) Column temperature: Ambient; Mobile phase components:
Component A: 2mM ammonium acetate Component B: methanol Flow rate: 300 pL/min Injection volume: 10 pL Solvent Gradient: 16.0 minutes
Time (minutes) %B
0.0 10% 1.0 10% 6.0 90% 11.0 100% 13.0 10%
Mass Spectrometer: Micromass API/Mass Spectrometer Quattro IITM Triple Quadrupole system Software: Mass Lynx 3.4 Cone Voltage: 30-60 V Collision Gas Energy: 25-45 eV Mode: Electrospray Negative Source Block Temperature: 150C 10C Electrode: Z-spray Analysis Type: Multiple Reaction Monitoring (MRM)
Target Ions Monitored in 3M Laboratory Analyses
Target Analyte
Primary Ion (AMU) Product Ion (AMU)
PFOS FOSA PFOA PFHS THPFOS1
499 498 413 399 427
80, 99 78 169 99 80
1 THPOS, Tetra-hydro perfluorooctanesulfonate, is used as a surrogate to monitor for gross instrument failure.
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Data Quality Objectives and Data Integrity The following data quality objectives were indicated in the method performance section of ETS-8-7.0, Analysis of Potassium Perfluorooctanesulfonate or Other Fluorochemicals in Liver Extracts Using HPLC-Electrospray/Mass Spectrometry:
Linearity: The coefficient of determination (r2) equal to or greater than 0.980. Limits of Quantitation (LOQ): The LOQ is equal to the lowest acceptable standard in the calibration curve. Acceptable Spike Recoveries: Matrix spikes prepared in rabbit liver must be recovered at 70-130% for the analytical run to be valid. Blanks: Matrix or surrogate matrix blanks must have a peak area < U the peak area of the lowest acceptable standard (LOQ).
Summary of Quality Control Analyses Results Linearity: The coefficient of determination (U) of the standard curve was >0.980. Calibration Standards: Quantitation of the target analytes was based on a quadratic curve weighted 1/x of a single, opening or two bracketing unextracted curves for each group of samples. High or low points on the curve may have been deactivated to provide a better fit over the curve range most appropriate to the data. Low curve points with peak areas less than two times that of the extraction blanks were deactivated to disqualify a data range that may have been significantly affected by background levels of the analyte. Occasionally, a single mid-range curve point that was an obvious outlier may have been deactivated. Quantitation of each analyte was based on the response of one or more specific product ion(s) using the multiple reaction-monitoring mode of the instrument. Calibration standards were prepared to run, undiluted, approximately within the linear range of the instrument (approximately 0.5-1000 ng/g).
Limits of Quantitation (LOQ): The LOQ is equal to the lowest acceptable standard in the calibration curve (defined as a standard within 30% of the theoretical value), and is at least two times the analyte peak area detected in the surrogate matrix blanks. Because low levels of the target analytes are ubiquitous in the laboratory, it is imperative that these criteria for LOQ determination be observed. The approximate LOQ for each analyte in each matrix is listed on the results summary table.
Blanks: All blanks were below the limit of quantitation for the compounds of interest, except for one water blank for PFHS and all caviar blanks for all compounds. These outliers will not adversely affect the outcome of this study. Given the nature of the samples encompassed by this study, the well-characterized matrix of rabbit liver was used as surrogate tissue for matrix blanks. These surrogate matrix blanks were < U the level of the LOQ.
Precision, Instrumental: Instrumental precision was not specifically determined within the course of this study. In other, similar liver/tissue studies, instrumental precision has been determined to be better than 7% for all target analytes.
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Matrix Spikes: At least one set of matrix spikes was prepared for each type of tissue from each type of animal; spikes were prepared at approximately 600 ng/g.
Recoveries of PFOS varied widely depending on the species and matrix. Therefore, adequate caution should be exercised in interpreting the results. Matrix spike recoveries are summarized in Appendix VII.
To verify the status of the extraction and analytical systems, matrix spikes were also prepared in a well-characterized surrogate matrix, rabbit liver. The matrix spike studies conducted in rabbit were all acceptable (+/-30%) for those analytical runs used when reporting data for samples and matrix spikes.
Demonstration of Specificity: Specificity for analyte identification was demonstrated by chromatographic retention time and mass spectral daughter ion characterization. Additional confirmatory tests were performed for PFOS. In the electrospray tandem mass spectrometry (ESMSMS) system, the 499 Da -> 80 Da transition can provide a stronger signal than the 499 Da -> 99 Da transition of the PFOS analysis. However, in the analysis of tissue samples collected from some species of animals, an unidentified interferent was present in the 499 Da 80 Da transition. Although this interferent is rarely observed, to ensure complete selectivity, quantitation was based on the 499 Da -> 99 Da transition.
To verify the identity of the detected analyte, in samples determined to contain PFOS at > 70 ng/g (liver or other tissue), at least 2 transitions were monitored and showed quantitative agreement to within +/-30%. Typically, 499>99 and 499>80 transitions were monitored. On the occasions that these two transitions differed by more than 30%, the sample was re-analyzed monitoring the 499>130 transition. This third transition showed quantitative agreement with the reported data from the 499>99 determination.
Statement of Data Quality It is not possible to verify true recovery of endogenous analyte from tissues without radiolabelled reference material. The only measurement of accuracy available at this time, matrix spike studies, indicate that the data can be reported with a stated accuracy of either +/-30%, +/-50%. The recoveries fall below 50%, reported data should be considered semi-quantitative. Details of matrix spike recoveries are summarized in Appendix VII. The concentrations of PFOS, FOSA, PFOA and PFHS were not corrected for the recoveries of the surrogate standard or matrix spike recoveries. The concentrations reported were not corrected for purity of the PFOS standard. Also, no attempt was made to correct the fluorochemical concentrations for moisture content in relevant tissues such as liver.
RESULTS AND DISCUSSION
Concentrations of PFOS, FOSA, PFOA and PFHS in fish tissues are given in Appendices I to VI and the results are summarized in Table 1. PFOS was detected in liver and eggs
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of fish from the Great Lakes. Concentrations were less than 400 ng/g, wet wt in the eggs of whitefish. Muscle tissues contained relatively less concentrations compared to those of liver or eggs. Among muscle tissues, carp from Saginaw Bay contained up to 297 ng/g, wet wt. Muscle tissues of fish from various inland lakes in Michigan did not contain quantifiable levels of PFOS. FOSA, PFOA and PFHS were rarely found in fish tissues. Among fishes analyzed from European countries, muscle of a fish from Scheldtz estuary in Belgium contained up to 923 ng/g, wet wt. This is the highest concentration of PFOS found in fishes monitored. Salmon and other fishes from the Baltic Sea did not contain quantifiable concentrations of PFOS. Among the samples from remote marine locations such as the North Pacific Ocean and the Antarctic Ocean, PFOS and other fluorochemicals were not detected.
REFERENCES (1) 3M Environmental Laboratory, St. Paul, MN LIMs Report numbers; E00-1634,
E00-1386, W3197, E00-2456, E00-2706, E01-0118.
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Table 1. Summary of concentrations (ng/g, wet wt or ng/mL) of PFOS, FOSA, PFOA and PFHS in fish tissues (Mean and Ranges)
Species
Tissue
Location
PFOS
FOSA
PFOA
PFHS
Yellowfin tuna (n=12)
Liver
North Pacific Ocean
<70
<19 <72 <17
Chinook salmon (n=6)
Liver
Great Lakes/inland lakes,
100 (32-173)
<19
<72 <17
Michigan
Lake whitefish (n=5)
Liver
Great Lakes/ inland lakes,
67 (33-81)
<19
<72 <17
Michigan
Brown trout (n=10)
Liver
Great Lakes/ inland lakes,
<17-26*
<19
<72 <17
Michigan
Carp diet (n=4)
Wholefish
Saginaw Bay, Michigan
<17-28*
<19
<72 <17
Lake whitefish (n=2)
Eggs
Great Lakes/ inland lakes,
263 (145-381)
<19
<36 <34
Michigan
Brown trout (n=3)
Eggs
Great Lakes/ inland lakes,
64 (49-75)
<19
<18 <34
Michigan
Carp (n=10)
Muscle
Saginaw Bay, Michigan
124 (59-297)
<19
<36 <34
Chinook salmon (n=6)
Muscle
Great Lakes/ inland lakes, Michigan
107 (<7-189)
<19
<36 <34
Lake whitefish (n=5)
Muscle
Great Lakes/ inland lakes,
132 (97-168)
<19
<36 <34
Michigan
Brown trout (n=10)
Muscle
Great Lakes/ inland lakes,
<7-46*
<19
<36 <34
Michigan
Swordfish (n=5)
Liver
Mediterranean Sea
<7-13*
<38 <36 <7-10*
Bluefin tuna (n=8)
Liver
Mediterranean Sea
48 (<3.5-87)
<38
<36 <7
Bluefin tuna (n=6)
Blood
Mediterranean Sea
40 (27-52)
15 (13-19)
<2.5
<1
Swordfish (n=7)
Blood
Mediterranean Sea
10 (4-21)
16 (4-26)
<2.5
<1
Silverfish, krill and
Wholefish
Antarctica
<19 <75 <37 <19
Trematomus (n=5)
Salmon (n=22)
Liver
Baltic Sea
<7.5 <19 <19 <7.5
Various species (n=26)
Muscle
Scheldtz estuary, Belgium
279 (<19-923) 40 (<3.7-122) 17 (<7.5-46) 10 (<3.7-20)
Various species (n=14)
Muscle, 1 liver
Poland
<19 <3.7 <7.5 <3.7
Various species (n=19) Muscle, 4
Czech republic
62 (<19-123)
<19
<19 <19
liver
Various species (n=35)
Liver
Inland Lakes, Michigan
43 (<7.7-120)
<7.7
<8 <3.8
Various species (n=19)
Eggs
Inland Lakes, Michigan
69 (<7.7-222)
<7.7
<8 <3.8
Various species (n=5)
Testes
Inland Lakes, Michigan
<7.7
<7.7 <8 <3.8
Various species (n=25)
Muscle
Poland
<3.9 and X
<3.8
<8 <3.8
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Various species (n=103)*
Muscle
Inland lakes, Michigan
<7.7 and X
<7.7 <8 and X
*Only one or two samples above the limit of quantification - mean not calculated; Values below LOQ are denoted by `< ' Values below the detection limit were not included in the estimation of mean; X - detected, not quantified
<3.8
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APPENDIX I
Concentrations of (ug/g, wet wt) fluorochemicals in fish
Species Yellowfin tuna Yellowfin tuna Yellowfin tuna Yellowfin tuna Yellowfin tuna Yellowfin tuna Yellowfin tuna Yellowfin tuna Yellowfin tuna Yellowfin tuna Yellowfin tuna Yellowfin tuna Chinook Salmon
Chinook Salmon
Chinook Salmon
Chinook Salmon
Chinook Salmon
Chinook Salmon
Lake whitefish
Lake whitefish
Lake whitefish
Lab ID TU25* TU34* TU41* TU48* TU49 TU54* TU58 TU63* TU66 TU84 TU88 TU90 1999030-01
1999030-02
1999030-02-01
1999030-02-04
1999030-03-01
1999030-03-04
1999029-11
1999029-12
1999029-13
Tissue Liver Liver Liver Liver Liver Liver Liver Liver Liver Liver Liver Liver Liver
Liver
Liver
Liver
Liver
Liver
Liver
Liver
Liver
Location
North Pacific Ocean North Pacific Ocean
North Pacific Ocean North Pacific Ocean
North Pacific Ocean North Pacific Ocean
North Pacific Ocean North Pacific Ocean
North Pacific Ocean North Pacific Ocean
North Pacific Ocean North Pacific Ocean
Great Lakes, Michigan
Great Lakes, Michigan
Great Lakes, Michigan
Great Lakes, Michigan
Great Lakes, Michigan
Great Lakes, Michigan
Great Lakes, Michigan
Great Lakes, Michigan
Great Lakes, Michigan
PFOS <LOQ ( 0.0696) <LOQ ( 0.0696) <LOQ ( 0.0696) <LOQ ( 0.0696) <LOQ ( 0.0696) <LOQ ( 0.0696) <LOQ ( 0.0696) <LOQ ( 0.0696) <LOQ ( 0.0696) <LOQ ( 0.0696) <LOQ ( 0.00696) <LOQ ( 0.0696)
0.109
0.169
0.032
0.126
0.173
0.041
0.068
0.081
0.074
PFHS <LOQ ( 0.0171) <LOQ ( 0.0171) <LOQ ( 0.0171) <LOQ ( 0.0171) <LOQ ( 0.0171) <LOQ ( 0.0171) <LOQ ( 0.0171) <LOQ ( 0.0171) <LOQ ( 0.0171) <LOQ ( 0.0171) <LOQ ( 0.0171) <LOQ ( 0.0171) <LOQ ( 0.0171)
<LOQ ( 0.0171)
<LOQ ( 0.0171)
<LOQ ( 0.0171)
<LOQ ( 0.0171)
<LOQ ( 0.0171)
<LOQ ( 0.0171)
<LOQ ( 0.0171)
<LOQ ( 0.0171)
PFOA <LOQ (0.0719) <LOQ (0.0719) <LOQ (0.0719) <LOQ (0.0719) <LOQ (0.0719) <LOQ (0.0719) <LOQ (0.0719) <LOQ (0.0719) <LOQ (0.0719) <LOQ (0.0719) <LOQ (0.0719) <LOQ (0.0719) <LOQ (0.0719)
<LOQ (0.0719)
<LOQ (0.0719)
<LOQ (0.0719)
<LOQ (0.0719)
<LOQ (0.0719)
<LOQ (0.0719)
<LOQ (0.0719)
<LOQ (0.0719)
FOSA <LOQ ( 0.0188) <LOQ ( 0.0188) <LOQ ( 0.0188) <LOQ ( 0.0188) <LOQ ( 0.0188) <LOQ ( 0.0188) <LOQ ( 0.0188) <LOQ ( 0.0188) <LOQ ( 0.0188) <LOQ ( 0.0188) <LOQ ( 0.0188) <LOQ ( 0.0188) <LOQ ( 0.0188)
<LOQ ( 0.0188)
<LOQ ( 0.0188)
<LOQ ( 0.0188)
<LOQ ( 0.0188)
<LOQ ( 0.0188)
<LOQ ( 0.0188)
<LOQ ( 0.0188)
<LOQ ( 0.0188)
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Lake whitefish Lake whitefish Brown Trout Brown Trout Brown Trout Brown Trout Brown Trout Brown Trout Brown Trout Brown Trout Brown Trout Brown Trout
1999029-14 1999029-16 1999040-01 1999040-02 1999040-03 1999040-04 1999040-05 1999040-06 1999040-07 1999040-08 1999040-09 1999040-10
Liver Liver Liver Liver Liver Liver Liver Liver Liver Liver Liver Liver
Carp Diet 1 (Carp5) ** Whole Carp Diet 2 (Carp) Whole Carp Diet 3 (Carp4) Whole Carp Diet 4 (Carp3) Whole
Great Lakes, Michigan
Great Lakes, Michigan
Great Lakes, Michigan
Great Lakes, Michigan
Great Lakes, Michigan
Great Lakes, Michigan
Great Lakes, Michigan
Great Lakes, Michigan
Great Lakes, Michigan
Great Lakes, Michigan
Great Lakes, Michigan
Great Lakes, Michigan
Saginaw Bay, MI Saginaw Bay, MI Saginaw Bay, MI Saginaw Bay, MI
Lake Whitefish Lake Whitefish
Brown Trout Brown Trout Brown Trout
1999029-13 1999029-14 1999040-01 1999040-04 1999040-06
Eggs Eggs Eggs Eggs Eggs
Great Lakes, MI Great Lakes, MI Great Lakes, MI Great Lakes, MI Great Lakes, MI
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0.033
0.078
<LOQ ( 0.0174)
<LOQ ( 0.0174)
<LOQ ( 0.0174)
<LOQ ( 0.0174)
0.026
<LOQ ( 0.0174)
<LOQ ( 0.0174)
<LOQ ( 0.0174)
<LOQ ( 0.0174)
<LOQ ( 0.0174)
<LOQ (0.0174) <LOQ (0.0174)
0.027 0.028
0.145 0.381 0.075 0.068 0.049
<LOQ ( 0.0171) <LOQ ( 0.0171) <LOQ ( 0.0171) <LOQ ( 0.0171) <LOQ ( 0.0171) <LOQ ( 0.0171) <LOQ ( 0.0171) <LOQ ( 0.0171) <LOQ ( 0.0171) <LOQ ( 0.0171) <LOQ ( 0.0171) <LOQ ( 0.0171)
<LOQ (0.0719) <LOQ (0.0719) <LOQ (0.0719) <LOQ (0.0719) <LOQ (0.0719) <LOQ (0.0719) <LOQ (0.0719) <LOQ (0.0719) <LOQ (0.0719) <LOQ (0.0719) <LOQ (0.0719) <LOQ (0.0719)
<LOQ ( 0.0188) <LOQ ( 0.0188) <LOQ ( 0.0188) <LOQ ( 0.0188) <LOQ ( 0.0188) <LOQ ( 0.0188) <LOQ ( 0.0188) <LOQ ( 0.0188) <LOQ ( 0.0188) <LOQ ( 0.0188) <LOQ ( 0.0188) <LOQ ( 0.0188)
<LOQ (0.0342) <LOQ (0.0342) <LOQ (0.0342) <LOQ (0.0342)
<LOQ (0.0342) <LOQ (0.0342) <LOQ (0.0342) <LOQ (0.0342) <LOQ (0.0342)
<LOQ ( 0.0359) <LOQ ( 0.0359) <LOQ ( 0.0359) <LOQ ( 0.0359)
<LOQ ( 0.0180) <LOQ ( 0.0180) <LOQ ( 0.0180) <LOQ ( 0.0180) <LOQ ( 0.0180)
<LOQ ( 0.0188) <LOQ ( 0.0188) <LOQ ( 0.0188) <LOQ ( 0.0188)
<LOQ ( 0.0188) <LOQ ( 0.0188) <LOQ ( 0.0188) <LOQ ( 0.0188) <LOQ ( 0.0188)
il
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Carp
B1N1
Muscle Saginaw Bay, MI
0.101
Carp
B1N2
Muscle Saginaw Bay, MI
0.078
Carp
B1N7
Muscle Saginaw Bay, MI
0.091
Carp
B1N10
Muscle Saginaw Bay, MI
0.088
Carp
B2N2
Muscle Saginaw Bay, MI
0.105
Carp
B2N6
Muscle Saginaw Bay, MI
0.089
Carp
B2N8
Muscle Saginaw Bay, MI
0.059
Carp
B2N10
Muscle Saginaw Bay, MI
0.084
Carp
Carp1*
Muscle Saginaw Bay, MI
0.297
Carp
Carp2*
Muscle Saginaw Bay, MI
0.243
Chinook Salmon
1999030-01
Chinook Salmon
1999030-02
Chinook Salmon 199903 0-02-01
Chinook Salmon Chinook Salmon Chinook Salmon
1999030-02-04 1999030-03-01 1999030-03-04
Lake Whitefish Lake Whitefish
1999029-11* 1999029-12*
Lake Whitefish Lake Whitefish
1999029-13 1999029-14
Lake Whitefish Brown trout
1999029-16 1999040-01
Brown trout Brown trout
1999040-02 ** 1999040-03
Brown trout Brown trout
1999040-04 1999040-05
Brown trout Brown trout
1999040-06 1999040-07
Brown trout Brown trout Brown trout
1999040-08 1999040-09 1999040-10
PFOS = Perfluorooctanesulfonate PFHS = Perfluorohexanesulfonate
Muscle Muscle Muscle Muscle Muscle Muscle Muscle Muscle Muscle Muscle Muscle Muscle Muscle Muscle Muscle Muscle Muscle Muscle Muscle Muscle Muscle
Great Lakes, MI
0.189
Great Lakes, MI Great Lakes, MI
0.126 <LOQ ( 0.00696)
Great Lakes, MI Great Lakes, MI Great Lakes, MI
0.113 0.051 0.057
Great Lakes, MI Great Lakes, MI
0.168 0.130
Great Lakes, MI Great Lakes, MI
0.097 0.098
Great Lakes, MI Great Lakes, MI
0.166 <LOQ ( 0.00696)
Great Lakes, MI Great Lakes, MI
<LOQ ( 0.00696) <LOQ ( 0.00696)
Great Lakes, MI Great Lakes, MI
<LOQ ( 0.00696) <LOQ ( 0.00696)
Great Lakes, MI Great Lakes, MI
<LOQ ( 0.00696) <LOQ ( 0.00696)
Great Lakes, MI Great Lakes, MI Great Lakes, MI
0.046 <LOQ ( 0.00696) <LOQ ( 0.00696)
FOSA = Perfluorooctanesulfonamide PFOA = Perfluorooctanoate
<LOQ ( 0.0342) <LOQ ( 0.0342) <LOQ ( 0.0342) <LOQ ( 0.0342) <LOQ ( 0.0342) <LOQ ( 0.0342) <LOQ ( 0.0342) <LOQ ( 0.0342) <LOQ ( 0.0342) <LOQ ( 0.0342)
<LOQ ( 0.0359) <LOQ ( 0.0359) <LOQ ( 0.0359) <LOQ ( 0.0359) <LOQ ( 0.0359) <LOQ ( 0.0359) <LOQ ( 0.0359) <LOQ ( 0.0359) <LOQ ( 0.0359) <LOQ ( 0.0359)
<LOQ ( 0.0188) <LOQ ( 0.0188) <LOQ ( 0.0188) <LOQ ( 0.0188) <LOQ ( 0.0188) <LOQ ( 0.0188) <LOQ ( 0.0188) <LOQ ( 0.0188) <LOQ ( 0.0188) <LOQ ( 0.0188)
<LOQ ( 0.0342) <LOQ ( 0.0359) <LOQ ( 0.0188)
<LOQ ( 0.0342) <LOQ ( 0.0342)
<LOQ ( 0.0359) <LOQ ( 0.0359)
<LOQ ( 0.0188) <LOQ ( 0.0188)
<LOQ ( 0.0342) <LOQ ( 0.0342) <LOQ ( 0.0342)
<LOQ ( 0.0359) <LOQ ( 0.0359) <LOQ ( 0.0359)
<LOQ ( 0.0188) <LOQ ( 0.0188) <LOQ ( 0.0188)
<LOQ ( 0.0342) <LOQ ( 0.0342)
<LOQ ( 0.0359) <LOQ ( 0.0359)
<LOQ ( 0.0188) <LOQ ( 0.0188)
<LOQ ( 0.0342) <LOQ ( 0.0342)
<LOQ ( 0.0359) <LOQ ( 0.0359)
<LOQ ( 0.0188) <LOQ ( 0.0188)
<LOQ ( 0.0342) <LOQ ( 0.0342)
<LOQ ( 0.0359) <LOQ ( 0.0359)
<LOQ ( 0.0188) <LOQ ( 0.0188)
<LOQ ( 0.0342) <LOQ ( 0.0342)
<LOQ ( 0.0359) <LOQ ( 0.0359)
<LOQ ( 0.0188) <LOQ ( 0.0188)
<LOQ ( 0.0342) <LOQ ( 0.0342)
<LOQ ( 0.0359) <LOQ ( 0.0359)
<LOQ ( 0.0188) <LOQ ( 0.0188)
<LOQ ( 0.0342) <LOQ ( 0.0342)
<LOQ ( 0.0359) <LOQ ( 0.0359)
<LOQ ( 0.0188) <LOQ ( 0.0188)
<LOQ ( 0.0342) <LOQ ( 0.0342) <LOQ ( 0.0342)
<LOQ ( 0.0359) <LOQ ( 0.0359) <LOQ ( 0.0359)
<LOQ ( 0.0188) <LOQ ( 0.0188) <LOQ ( 0.0188)
LOQ = Limit of quantitation - values in parenthesis
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APPENDIX II
Concentrations of (ug/g, wet wt) fluorochemicals in fish
Species
Swordfish Swordfish Swordfish Swordfish Swordfish
Lab ID
S23 (23) S24 (24) S25 (25) S32 (27) S48 (26)
Tissue
Liver Liver Liver Liver Liver
Location
Mediterranean Sea Mediterranean Sea Mediterranean Sea Mediterranean Sea Mediterranean Sea
PFOS
PFOA
< LOQ (0.00696 ug/g) <LOQ (0.0359 ug/g) < LOQ (0.00696 ug/g) <LOQ (0.0359 ug/g)
0.008
<LOQ (0.0359 ug/g)
< LOQ (0.00696 ug/g) <LOQ (0.0359 ug/g)
0.013
<LOQ (0.0359 ug/g)
FOSA
<LOQ (0.0376 ug/g) <LOQ (0.0376 ug/g) <LOQ (0.0376 ug/g) <LOQ (0.0376 ug/g) <LOQ (0.0376 ug/g)
PFHS
0.010 < LOQ (0.00683 ug/g) < LOQ (0.00683 ug/g) < LOQ (0.00683 ug/g) < LOQ (0.00683 ug/g)
Tunafish Tunafish
Tunafish Tunafish Tunafish Tunafish Tunafish Tunafish
T1 (33) T1LF156
(35) T2 (29) T15 (28) T17 (31) T20 (32) T23 (30) T25 (34)
Liver Mediterranean Sea < LOQ (0.0347 ug/g) <LOQ (0.0359 ug/g) <LOQ (0.0376 ug/g) < LOQ (0.00683 ug/g)
Liver Mediterranean Sea
0.043
<LOQ (0.0718 ug/g) <LOQ (0.0376 ug/g) < LOQ (0.00683 ug/g)
Liver Liver Liver Liver Liver Liver
Mediterranean Sea Mediterranean Sea Mediterranean Sea Mediterranean Sea Mediterranean Sea Mediterranean Sea
0.087 0.057 0.049 0.021 0.056 0.025
<LOQ (0.0359 ug/g) <LOQ (0.0718 ug/g) <LOQ (0.0359 ug/g) <LOQ (0.0359 ug/g) <LOQ (0.0359 ug/g) <LOQ (0.0718 ug/g)
<LOQ (0.0376 ug/g) <LOQ (0.0376 ug/g) <LOQ (0.0376 ug/g) <LOQ (0.0376 ug/g) <LOQ (0.0376 ug/g) <LOQ (0.0376 ug/g)
< LOQ (0.00683 ug/g) < LOQ (0.00683 ug/g) < LOQ (0.00683 ug/g) < LOQ (0.00683 ug/g) < LOQ (0.00683 ug/g) < LOQ (0.00683 ug/g)
PFOS = Perfluorooctanesulfonate PFHS = Perfluorohexanesulfonate PFOA = Perfluorooctanoate
FOSA = Perfluorooctanesulfonamide
LOQ = Limit of quantitation - values in parenthesis
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Concentrations of (ng/mL) fluorochemicals in fish
Species
I.d.
Tissue
Location
APPENDIX III
PFOS
PFHS
PFOA
FOSA
Bluefin tuna Bluefin tuna Bluefin tuna Bluefin tuna Bluefin tuna Bluefin tuna
Swordfish Swordfish Swordfish Swordfish Swordfish Swordfish Swordfish
T15 (7) T02 (8) T17 (9) T23 (10) T25 (11) T20 (12) S06 (13) S08 (14) S13 (15) S18 (16) S21 (17) S36 (18) S38 (19)
Blood Blood Blood Blood Blood Blood Blood Blood Blood Blood Blood Blood Blood
Italian coast Italian coast Italian coast Italian coast Italian coast Italian coast Italian coast Italian coast Italian coast Italian coast Italian coast Italian coast Italian coast
0.037 0.052 0.035 0.051 0.027 0.036 0.021 0.006 0.014 0.008 0.006 0.013 0.004
<LOQ (1.00 ng/mL) <LOQ (1.00 ng/mL) <LOQ (1.00 ng/mL) <LOQ (1.00 ng/mL) <LOQ (1.00 ng/mL) <LOQ (1.00 ng/mL) <LOQ (1.00 ng/mL) <LOQ (1.00 ng/mL) <LOQ (1.00 ng/mL) <LOQ (1.00 ng/mL) <LOQ (1.00 ng/mL) <LOQ (1.00 ng/mL) <LOQ (1.00 ng/mL)
<LOQ (2.50 ng/mL) <LOQ (2.50 ng/mL) <LOQ (2.50 ng/mL) <LOQ (2.50 ng/mL) <LOQ (2.50 ng/mL) <LOQ (2.50 ng/mL) <LOQ (2.50 ng/mL) <LOQ (2.50 ng/mL) <LOQ (2.50 ng/mL) <LOQ (2.50 ng/mL) <LOQ (2.50 ng/mL) <LOQ (2.50 ng/mL) <LOQ (2.50 ng/mL)
0.013 0.013 0.013 0.019 0.014 0.017 0.006 0.011 0.014 0.025 0.028 0.004 0.026
PFOS = Perfluorooctanesulfonate PFHS = Perfluorohexanesulfonate PFOA = Perfluorooctanoate
FOSA = Perfluorooctanesulfonamide
LOQ = Limit of quantitation - values in parenthesis
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APPENDIX IV
Concentrations of (ug/g, wet wt) fluorochemicals in fish
Species
Lab ID
Trematomus pennelli (40) BTN-1
Trematomus pennelli (41) BTN-1
Silver fish
(42) Pa4
Silver fish
(43) Pa1
Krill (44) KRL001
Salmon Baltic/Finnish
Salmon Baltic/Finnish
Salmon Baltic/Finnish
Salmon Baltic/Finnish
Salmon Baltic/Finnish
Salmon Baltic/Finnish
Salmon Baltic/Finnish
Salmon Baltic/Finnish
Salmon Baltic/Finnish
Salmon Baltic/Finnish
Salmon -
(239) N14 (240) N32 (241) N16 (242) N13 (243) N11 (244) N29 (245) N9 (246) N2 (247) N20 (248) N3 (249) N18
Tissue Wholefish Wholefish Wholefish Wholefish Wholebody
Liver Liver Liver Liver Liver Liver Liver Liver Liver Liver Liver
Location Antarctica
Antarctica
Antarctica
Antarctica
Antarctica
Baltic Sea, Finnish coast
Baltic Sea, Finnish coast
Baltic Sea, Finnish coast
Baltic Sea, Finnish coast
Baltic Sea, Finnish coast
Baltic Sea, Finnish coast
Baltic Sea, Finnish coast
Baltic Sea, Finnish coast
Baltic Sea, Finnis h coast
Baltic Sea, Finnish coast
Baltic Sea, Finnish
PFOS
< LOQ (0.0188
ug/g) < LOQ (0.0188
ug/g) < LOQ (0.0188
ug/g) < LOQ (0.0188
ug/g) < LOQ (0.0188
ug/g) <LOQ (0.00750
ug/g) <LOQ (0.00750
ug/g) <LOQ (0.00750
ug/g) <LOQ (0.00750
ug/g) <LOQ (0.00750
ug/g) <LOQ (0.00750
ug/g) <LOQ (0.00750
ug/g) <LOQ (0.00750
ug/g) <LOQ (0.00750
ug/g) <LOQ (0.00750
ug/g) <LOQ (0.00750
FOSA < LOQ (0.0750 ug/g) < LOQ (0.0750 ug/g) < LOQ (0.0750 ug/g) < LOQ (0.0750 ug/g) < LOQ (0.0750 ug/g) <LOQ (0.0188 ug/g) <LOQ (0.0188 ug/g) <LOQ (0.0188 ug/g) <LOQ (0.0188 ug/g) <LOQ (0.0188 ug/g) <LOQ (0.0188 ug/g) <LOQ (0.0188 ug/g) <LOQ (0.0188 ug/g) <LOQ (0.0188 ug/g) <LOQ (0.0188 ug/g) <LOQ (0.0188 ug/g)
PFOA
< LOQ (0.0374 ug/g)
< LOQ (0.0374 ug/g)
< LOQ (0.0374 ug/g)
< LOQ (0.0374 ug/g)
< LOQ (0.0374 ug/g)
<LOQ (0.0188 ug/g)
<LOQ (0.0188 ug/g)
<LOQ (0.0188 ug/g)
<LOQ (0.0188 ug/g)
<LOQ (0.0188 ug/g)
<LOQ (0.0188 ug/g)
<LOQ (0.0188 ug/g)
<LOQ (0.0188 ug/g)
<LOQ (0.0188 ug/g)
<LOQ (0.0188 ug/g)
<LOQ (0.0188
PFHS
< LOQ (0.0188 ug/g)
< LOQ (0.0188 ug/g)
< LOQ (0.0188 ug/g)
< LOQ (0.0188 ug/g)
< LOQ (0.0188 ug/g)
<LOQ (0.00750 ug/g)
<LOQ (0.00750 ug/g)
<LOQ (0.00750 ug/g)
<LOQ (0.00750 ug/g)
<LOQ (0.00750 ug/g)
<LOQ (0.00750 ug/g)
<LOQ (0.00750 ug/g)
<LOQ (0.00750 ug/g)
<LOQ (0.00750 ug/g)
<LOQ (0.00750 ug/g)
<LOQ (0.00750
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Baltic/Finnish Salmon -
Baltic/Finnish Salmon -
Baltic/Finnish Salmon -
Baltic/Finnish Salmon -
Baltic/Finnish Salmon -
Baltic/Finnish Salmon -
Baltic/Finnish Salmon -
Baltic/Finnish Salmon -
Baltic/Finnish Salmon -
Baltic/Finnish Salmon -
Baltic/Finnish Salmon -
Baltic/Finnish
(250) N12 (251) N5 (252) MB985 (253) KR2169 (254) OV9582 (255) OV9581 (256) OV9596 (257) KR2168 (258) OV9698 (259) MB9897 (260) KR2179
PFOS = Perfluorooctanesulfonate PFHS = Perfluorohexanesulfonate
Liver Liver Liver Liver Liver Liver Liver Liver Liver Liver Liver
coast Baltic Sea, Finnish
coast Baltic Sea, Finnish
coast Baltic Sea, Finnish
coast Baltic Sea, Finnish
coast Baltic Sea, Finnish
coast Baltic Sea, Finnish
coast Baltic Sea, Finnish
coast Baltic Sea, Finnish
coast Baltic Sea, Finnish
coast Baltic Sea, Finnish
coast Baltic Sea, Finnish
coast
ug/g) <LOQ (0.00750
ug/g) <LOQ (0.00750
ug/g) <LOQ (0.00750
ug/g) <LOQ (0.00750
ug/g) <LOQ (0.00750
ug/g) <LOQ (0.00750
ug/g) <LOQ (0.00750
ug/g) <LOQ (0.00750
ug/g) <LOQ (0.00750
ug/g) <LOQ (0.00750
ug/g) <LOQ (0.00750
ug/g)
FOSA = Perfluorooctanesulfonamide PFOA = Perfluorooctanoate
<LOQ (0.0188 ug/g) <LOQ (0.0188 ug/g) <LOQ (0.0188 ug/g) <LOQ (0.0188 ug/g) <LOQ (0.0188 ug/g) <LOQ (0.0188 ug/g) <LOQ (0.0188 ug/g) <LOQ (0.0188 ug/g) <LOQ (0.0188 ug/g) <LOQ (0.0188 ug/g) <LOQ (0.0188 ug/g)
ug/g) <LOQ (0.0188
ug/g) <LOQ (0.0188
ug/g) <LOQ (0.0188
ug/g) <LOQ (0.0188
ug/g) <LOQ (0.0188
ug/g) <LOQ (0.0188
ug/g) <LOQ (0.0188
ug/g) <LOQ (0.0188
ug/g) <LOQ (0.0188
ug/g) <LOQ (0.0188
ug/g) <LOQ (0.0188
ug/g)
ug/g) <LOQ (0.00750
ug/g) <LOQ (0.00750
ug/g) <LOQ (0.00750
ug/g) <LOQ (0.00750
ug/g) <LOQ (0.00750
ug/g) <LOQ (0.00750
ug/g) <LOQ (0.00750
ug/g) <LOQ (0.00750
ug/g) <LOQ (0.00750
ug/g) <LOQ (0.00750
ug/g) <LOQ (0.00750
ug/g)
LOQ = Limit of quantitation - values in parenthesis
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APPENDIX V
Concentrations of (ug/g, wet wt) fluorochemicals in fish
Group
Fish Fish Fish Fish Fish Fish Fish Fish Fish Fish Fish Fish Fish Fish Fish Fish Fish Fish Fish Fish Fish Fish Fish Fish Fish Fish Fish
Sample #
(1) BW7-2 (2) BW8-2 (3) BW9-2 (4) BW6-N2 (5) BW17-2 (6) BW11 (7) BW12 (8) BW13 (9) BW16-2 (10) BW15-2 (11) BW18-2 (12) BW22-2 (13) BW19-2 (14) BW24 (15) BW23-3 (16) BW19-1 (17) BW20-2 (18) BW21-2 (19) CV22-2 (20) CV25-2 (21) CV13-2 (22) CV3-2 (23) CV26-B2 (24) CV12-2 (25) CV2-2 (26) CV26A2 (27) poland4
Tissue
Muscle Muscle Muscle Muscle Muscle Muscle Muscle Muscle Muscle Muscle Muscle Muscle Muscle Muscle Muscle Muscle Muscle Muscle Muscle Muscle Muscle Muscle Muscle Muscle Muscle Muscle Muscle
Location
Belgium Belgium Belgium Belgium Belgium Belgium Belgium Belgium Belgium Belgium Belgium Belgium Belgium Belgium Belgium Belgium Belgium Belgium Belgium Belgium Belgium Belgium Belgium Belgium Belgium Belgium Poland
PFOS
FOSA
PFOA
PFHS
ug/ g 0.0505 0.606 0.0879 0.206 0.199 0.0399 0.0612 0.0429 0.923 0.347 0.420 0.268 0.467 0.0987 0.529 0.327 0.285 0.134 <LOQ (0.0188 ug/g) <LOQ (0.0188 ug/g) <LOQ (0.0188 ug/g) <LOQ (0.0188 ug/g) 0.637 <LOQ (0.0188 ug/g) 0.0845 0.0409 <LOQ (0.0188 ug/g)
u g/ g 0.0162 0.0435 0.0112 0.00724 0.0463 0.0461 0.0192 0.0494 0.0636 0.0819 0.0228 0.0562 0.0762 0.122 0.0922 0.0512 0.0265 0.0393 0.00944 0.00827 <LOQ (0.00375 ug/g) 0.00528 0.0220 <LOQ (0.00375 ug/g) <LOQ (0.00375 ug/g) 0.00683 <LOQ (0.00375 ug/g)
u g/ g <LOQ (0.00750 ug/g) <LOQ (0.00750 ug/g) <LOQ (0.00750 ug/g) <LOQ (0.00750 ug/g) <LOQ (0.00750 ug/g) <LOQ (0.00750 ug/g) <LOQ (0.00750 ug/g) <LOQ (0.00750 ug/g) <LOQ (0.00750 ug/g) <LOQ (0.00750 ug/g)
0.0145 0.0142 0.0127 <LOQ (0.00750 ug/g) <LOQ (0.00750 ug/g) <LOQ (0.00750 ug/g) 0.0461 0.0186 <LOQ (0.00750 ug/g) <LOQ (0.00750 ug/g) <LOQ (0.00750 ug/g) 0.00902 <LOQ (0.00750 ug/g) 0.00782 <LOQ (0.00750 ug/g) 0.0116 <LOQ (0.00750 ug/g)
u g/ g <LOQ (0.00375 ug/g)
0.0205 <LOQ (0.00375 ug/g) <LOQ (0.00375 ug/g)
0.00831 <LOQ (0.00375 ug/g)
0.00452 <LOQ (0.00375 ug/g)
0.00943 0.00640 <LOQ (0.00375 ug/g) 0.0139 0.0170 0.00754 0.00543 0.0155 0.00509 0.0116 <LOQ (0.00375 ug/g) <LOQ (0.00375 ug/g) <LOQ (0.00375 ug/g) <LOQ (0.00375 ug/g) 0.00681 <LOQ (0.00375 ug/g) <LOQ (0.00375 ug/g) <LOQ (0.00375 ug/g) <LOQ (0.00375 ug/g)
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Fish (28) poland18 Muscle
Fish
(29) poland5
Muscle
Fish
(30) poland2
Muscle
Fish
(31) poland3
Muscle
Fish (32) polandburbot Liver
Fish (33) polandfluonder Muscle
Fish (34) polandpelceus Muscle
Fish (35) polandturbot Muscle Fish (36) polandflounder Muscle
Fish (37) polandsmelt Muscle
Fish (38) polandpike Muscle
Fish (39) polandroach Muscle
Fish (40) polandperch Muscle
Fish
(41) BA-1
Muscle
Fish
(42) BA-2
Muscle
Fish
(43) P-1
Muscle
Fish
(44) C-3
Muscle
Fish
(45) C-4
Muscle
Fish
(46) BR-1
Muscle
Fish
(47) BR-2
Muscle
Fish (52) polandburbot-13 Muscle
Fish
(53) catfish
Muscle
Fish
(54)BR-3
Liver
Fish
(55) CA-1M
Muscle
Fish
(56) CA-1L
Liver
Fish
(57) C-1M
Muscle
Fish
(58) C-1L
Liver
Fish
(59) C-2M
Muscle
Fish
(60) C-2L
Liver
Fish
(61) CA-2M
Muscle
Fish
(63) CA-3M
Muscle
Fish
(64) CA-3L
Liver
PFOS = Perfluorooctanesulfonate
Poland
<LOQ (0.0188 ug/g) <LOQ (0.00375 ug/g) <LOQ (0.00750 ug/g) <LOQ (0.00375 ug/g)
Poland
<LOQ (0.0188 ug/g) <LOQ (0.00375 ug/g) <LOQ (0.00750 ug/g) <LOQ (0.00375 ug/g)
Poland
<LOQ (0.0188 ug/g) <LOQ (0.00375 ug/g) <LOQ (0.00750 ug/g) <LOQ (0.00375 ug/g)
Poland
<LOQ (0.0188 ug/g) <LOQ (0.00375 ug/g) <LOQ (0.00750 ug/g) <LOQ (0.00375 ug/g)
Poland
<LOQ (0.0188 ug/g) <LOQ (0.00375 ug/g) <LOQ (0.00750 ug/g) <LOQ (0.00375 ug/g)
Poland
<LOQ (0.0188 ug/g) <LOQ (0.00375 ug/g) <LOQ (0.00750 ug/g) <LOQ (0.00375 ug/g)
Poland
<LOQ (0.0188 ug/g) <LOQ (0.00375 ug/g) <LOQ (0.00750 ug/g) <LOQ (0.00375 ug/g)
Poland Poland
<LOQ (0.0188 ug/g) <LOQ (0.00375 ug/g) <LOQ (0.00750 ug/g) <LOQ (0.00375 ug/g) <LOQ (0.0188 ug/g) <LOQ (0.00375 ug/g) <LOQ (0.00750 ug/g) <LOQ (0.00375 ug/g)
Poland
<LOQ (0.0188 ug/g) <LOQ (0.00375 ug/g) <LOQ (0.00750 ug/g) <LOQ (0.00375 ug/g)
Poland
<LOQ (0.0188 ug/g) <LOQ (0.00375 ug/g) <LOQ (0.00750 ug/g) <LOQ (0.00375 ug/g)
Poland
<LOQ (0.0188 ug/g) <LOQ (0.00375 ug/g) <LOQ (0.00750 ug/g) <LOQ (0.00375 ug/g)
Poland
<LOQ (0.0188 ug/g) <LOQ (0.00375 ug/g) <LOQ (0.00750 ug/g) <LOQ (0.00375 ug/g)
Czech Republic <LOQ (0.0188 ug/g) <LOQ (0.00375 ug/g) <LOQ (0.00750 ug/g) <LOQ (0.00375 ug/g)
Czech Republic <LOQ (0.0188 ug/g) <LOQ (0.00375 ug/g) <LOQ (0.00750 ug/g) <LOQ (0.00375 ug/g)
Czech Republic <LOQ (0.0188 ug/g) <LOQ (0.00375 ug/g) <LOQ (0.00750 ug/g) <LOQ (0.00375 ug/g)
Czech Republic <LOQ (0.0188 ug/g) <LOQ (0.00375 ug/g) <LOQ (0.00750 ug/g) <LOQ (0.00375 ug/g)
Czech Republic <LOQ (0.0188 ug/g) <LOQ (0.00375 ug/g) <LOQ (0.00750 ug/g) <LOQ (0.00375 ug/g)
Czech Republic <LOQ (0.0188 ug/g) < LOQ (0.0188 ug/g) <LOQ (0.0188 ug/g) <LOQ (0.0188 ug/g)
Czech Republic
0.0223
< LOQ (0.0188 ug/g) <LOQ (0.0188 ug/g) <LOQ (0.0188 ug/g)
Czech Republic < LOQ (0.0188 ug/g) < LOQ (0.0188 ug/g) < LOQ (0.0188 ug/g) < LOQ (0.0188 ug/g)
Czech Republic < LOQ (0.0188 ug/g) < LOQ (0.0188 ug/g) < LOQ (0.0188 ug/g) < LOQ (0.0188 ug/g)
Czech Republic
0.0255
< LOQ (0.0188 ug/g) < LOQ (0.0188 ug/g) < LOQ (0.0188 ug/g)
Czech Republic < LOQ (0.0188 ug/g) < LOQ (0.0188 ug/g) < LOQ (0.0188 ug/g) < LOQ (0.0188 ug/g)
Czech Republic < LOQ (0.0188 ug/g) < LOQ (0.0188 ug/g) < LOQ (0.0188 ug/g) < LOQ (0.0188 ug/g)
Czech Republic
0.123
< LOQ (0.0188 ug/g) < LOQ (0.0188 ug/g) < LOQ (0.0188 ug/g)
Czech Republic
0.0856
< LOQ (0.0188 ug/g) < LOQ (0.0188 ug/g) < LOQ (0.0188 ug/g)
Czech Republic < LOQ (0.0188 ug/g) < LOQ (0.0188 ug/g) < LOQ (0.0188 ug/g) < LOQ (0.0188 ug/g)
Czech Republic
0.0538
< LOQ (0.0188 ug/g) < LOQ (0.0188 ug/g) < LOQ (0.0188 ug/g)
Czech Republic < LOQ (0.0188 ug/g) < LOQ (0.0188 ug/g) < LOQ (0.0375 ug/g) < LOQ (0.0188 ug/g)
Czech Republic < LOQ (0.0188 ug/g) < LOQ (0.0188 ug/g) < LOQ (0.0375 ug/g) < LOQ (0.0188 ug/g)
Czech Republic < LOQ (0.0188 ug/g) < LOQ (0.0188 ug/g) < LOQ (0.0375 ug/g) < LOQ (0.0188 ug/g)
FOSA = Perfluorooctanesulfonamide
LOQ = Limit of quantitation - values in parenthesis
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PFHS = Perfluorohexanesulfonate
PFOA = Perfluorooctanoate
APPENDIX VI
Concentrations of (ug/g, wet wt) fluorochemicals in fish
Species Fish Liver Fish Liver Fish Liver Fish Liver Fish Liver Fish Liver Fish Liver Fish Liver Fish Liver Fish Liver Fish Liver Fish Liver Fish Liver Fish Liver Fish Liver
Sample id 6B (2000021-17) 7B (2000021-14) 10B (2000021-18) 12B (2000021-19) 19B (2000021-16) 23B (2000021-20) 26B (2000047-15) 28B (2000047-16) 30B (2000047-17) 35B (2000104-20) 37B (2000104-17) 45B (2000020-09) 58B (2000020-04) 59B (2000020-03) 60B (2000020-01)
Tissue Liver Liver Liver Liver Liver Liver Liver Liver Liver Liver Liver Liver Liver Liver Liver
Location Michigan inland Lakes Michigan inland Lakes Michigan inland Lakes Michigan inland Lakes Michigan inland Lakes Michigan inland Lakes Michigan inland Lakes Michigan inland Lakes Michigan inland Lakes Michigan inland Lakes Michigan inland Lakes Michigan inland Lakes Michigan inland Lakes Michigan inland Lakes Michigan inland Lakes
PFOS 0.0351
FOSA
PFOA
<LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g)
0.0419
<LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g)
0.0093
<LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g)
0.0266
<LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g)
0.0293
<LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g)
0.0494
<LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g)
0.0546
<LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g)
<LOQ (0.00773 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g)
<LOQ (0.00773 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g)
0.0151
<LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g)
<LOQ (0.00773 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g)
<LOQ (0.00773 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g)
0.0333
<LOQ (0.0038 ug/g) <LOQ (0.0201 ug/g)
0.0115
<LOQ (0.0038 ug/g) <LOQ (0.0201 ug/g)
0.0068
<LOQ (0.0038 ug/g) <LOQ (0.0201 ug/g)
PFHS
<LOQ (0.0038 ug/g)
<LOQ (0.0038 ug/g)
<LOQ (0.0038 ug/g)
<LOQ (0.0038 ug/g)
<LOQ (0.0038 ug/g)
<LOQ (0.0038 ug/g)
<LOQ (0.0038 ug/g)
<LOQ (0.0038 ug/g)
<LOQ (0.0038 ug/g) X
<LOQ (0.0038 ug/g)
<LOQ (0.0038 ug/g)
<LOQ (0.0038 ug/g)
<LOQ (0.0038 ug/g)
<LOQ (0.0038
19
Fish Liver 61B (2000020-02) Liver Michigan inland Lakes Fish Liver 67B (20000303-01) Liver Michigan inland Lakes Fish Liver 75B (20000303-04) Liver Michigan inland Lakes Fish Liver 76B (20000302-02) Liver Michigan inland Lakes Fish Liver 79B (20000303-03) Liver Michigan inland Lakes Fish Liver 83B (20000303-02) Liver Michigan inland Lakes Fish Liver 88B (20000302-04) Liver Michigan inland Lakes Fish Liver 90B (20000303-05) Liver Michigan inland Lakes Fish Liver 95B (20000302-05) Liver Michigan inland Lakes Fish Liver 105 (200003301-02) Liver Michigan inland Lakes Fish Liver 108 (20000301-04) Liver Michigan inland Lakes Fish Liver 112 (20000302-03) Liver Michigan inland Lakes Fish Liver 114 (20000301-05) Liver Michigan inland Lakes Fish Liver 116 (200003301-03) Liver Michigan inland Lakes Fish Liver 117 (200003301-01) Liver Michigan inland Lakes Fish Liver 119 (20000301-01) Liver Michigan inland Lakes Fish Liver 128 (2000071-03) Liver Michigan inland Lakes Fish Liver 131 (2000071-04) Liver Michigan inland Lakes
BACK TO MAIN
ug/g)
<LOQ (0.0039 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038
0.0518
ug/g) <LOQ (0.0038 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038
0.0859
ug/g) <LOQ (0.0038 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038
ug/g) <LOQ (0.0039 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038
0.0174
ug/g) <LOQ (0.0038 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038
0.0951
ug/g) <LOQ (0.0038 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038
0.0918
ug/g) <LOQ (0.0038 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038
0.120
ug/g) <LOQ (0.0038 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038
0.0129
ug/g) <LOQ (0.0038 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038
0.0200
ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00750
0.0145
ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00750
0.0144
ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00750
0.0281
ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00750
ug/g) <LOQ (0.00773 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00750
0.0216
ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00750
0.00837
ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00750
0.114
ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00750
0.0382
ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00750
ug/g)
20
Fish Liver 132 (2000071-05) Liver Michigan inland Lakes
Fish Liver 135 (2000071-07) Liver Michigan inland Lakes
Fish Eggs 4B (2000021-17) Eggs Michigan inland Lakes
Fish Eggs
9B (2000021-18)
Eggs Michigan inland Lakes
Fish Eggs 18B (2000021-16) Eggs Michigan inland Lakes
Fish Eggs 21B (2000021-20) Eggs Michigan inland Lakes
Fish Eggs 24B (2000047-14) Eggs Michigan inland Lakes
Fish Eggs 42B (2000020-05) Eggs Michigan inland Lakes
Fish Eggs 5OB (2000020-10) Eggs Michigan inland Lakes
Fish Eggs 52B (2000020-07) Eggs Michigan inland Lakes
Fish Eggs 55B (2000020-03) Eggs Michigan inland Lakes
Fish Eggs 57B (2000020-06) Eggs Michigan inland Lakes
Fish Eggs 70B (20000302-03) Eggs Michigan inland Lakes
Fish Eggs 78B (20000303-03) Eggs Michigan inland Lakes
Fish Eggs 81B (20000303-01) Eggs Michigan inland Lakes
Fish Eggs 92B (20000302-01) Eggs Michigan inland Lakes
Fish Eggs 106 (20000302-05) Eggs Michigan inland Lakes
Fish Eggs 107 (20000302-02) Eggs Michigan inland Lakes
Fish Eggs 124 (2000071-02) Eggs Michigan inland Lakes
BACK TO MAIN
0.0555
<LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00750
0.113
ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00750
0.0199
ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.0038
<LOQ (0.00773 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00773 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00773 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g)
ug/g) <LOQ (0.0038
ug/g) <LOQ (0.0038
ug/g) <LOQ (0.0038
0.0284
ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.0038
0.00789
ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.0038
ug/g) <LOQ (0.00773 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00750
ug/g) <LOQ (0.0039 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038
0.0136
ug/g) <LOQ (0.0038 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038
0.00898
ug/g) <LOQ (0.0038 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038
0.222
ug/g) <LOQ (0.0038 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038
0.126
ug/g) <LOQ (0.0038 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038
0.127
ug/g) <LOQ (0.0038 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038
0.142
ug/g) <LOQ (0.0038 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038
0.0447
ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00750
0.0747
ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00750
0.0229
<LOQ (0.00765 ug/g)
0.0137
ug/g) <LOQ (0.00750
21
Fish Eggs 138 (2000071-09) Eggs Michigan inland Lakes
Fish Eggs 141 (2000071-10) Eggs Michigan inland Lakes
Fish Testes 74B (20000303-04) Testes Michigan inland Lakes
Fish Testes 82B (20000303-02) Testes Michigan inland Lakes
Fish Testes 85B (20000302-04) Testes Michigan inland Lakes
Fish Testes 101 (200003301-02) Testes Michigan inland Lakes
Fish Testes 109 (20000301-04) Testes Michigan inland Lakes
Fish Muscle
1 (348)
Muscle
Poland
Fish Muscle
2(374)
Muscle
Poland
Fish Muscle
3 (375)
Muscle
Poland
Fish Muscle
4 (373)
Muscle
Poland
Fish Muscle
5 (365)
Muscle
Poland
Fish Muscle
6(349)
Muscle
Poland
Fish Muscle
7 (354)
Muscle
Poland
Fish Muscle
8 (355)
Muscle
Poland
Fish Muscle
9(327)
Muscle
Poland
Fish Muscle
10(329)
Muscle
Poland
Fish Muscle
11 (341)
Muscle
Poland
BACK TO MAIN
ug/g)
0.0271
<LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00750
0.0949
ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00750
ug/g) <LOQ (0.0039 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038
ug/g) <LOQ (0.0039 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038
ug/g) <LOQ (0.0039 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038
ug/g) <LOQ (0.00773 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00750
ug/g) <LOQ (0.00773 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00750
ug/g) X <LOQ (0.0038 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.0038
ug/g) X <LOQ (0.0038 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.0038
ug/g) < LOQ (0.0039 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.0038
ug/g) < LOQ (0.0039 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.0038
ug/g) < LOQ (0.0039 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.0038
ug/g) < LOQ (0.0039 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.0038
ug/g) < LOQ (0.0039 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.0038
ug/g) < LOQ (0.0039 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.0038
ug/g) < LOQ (0.0039 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.0038
ug/g) X <LOQ (0.0038 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.0038
ug/g) X <LOQ (0.0038 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.0038
ug/g)
22
Fish Muscle
12(2)
Muscle
Poland
Fish Muscle
13 (360/376)
Muscle
Poland
Fish Muscle
14 (377)
Muscle
Poland
Fish Muscle
15 (326)
Muscle
Poland
Fish Muscle
16(1)
Muscle
Poland
Fish Muscle
17 (339)
Muscle
Poland
Fish Muscle
18(340)
Muscle
Poland
Fish Muscle
19 (333)
Muscle
Poland
Fish Muscle
20(336)
Muscle
Poland
Fish Muscle
21 (345)
Muscle
Poland
Fish Muscle
22(330)
Muscle
Poland
Fish Muscle
23 (331)
Muscle
Poland
Fish Muscle
24(332)
Muscle
Poland
Fish Muscle
25 (370)
Muscle
Poland
Fish Muscle IB (2000021-01to03) Muscle Michigan inland Lakes
Fish Muscle 2B (2000021-07tol0) Muscle Michigan inland Lakes
Fish Muscle 3B (200002l-04to06) Muscle Michigan inland Lakes
Fish Muscle 5B (2000021-17) Muscle Michigan inland Lakes
Fish Muscle 8B (2000021-14) Muscle Michigan inland Lakes
BACK TO MAIN
< LOQ (0.0039 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.00803 ug/g) < LOQ (0.0039 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.00803 ug/g) < LOQ (0.0039 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.00803 ug/g) < LOQ (0.0039 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.00803 ug/g) < LOQ (0.0039 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.00803 ug/g) < LOQ (0.0039 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.00803 ug/g) < LOQ (0.0039 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.00803 ug/g) < LOQ (0.0039 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.00803 ug/g) < LOQ (0.0039 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.00803 ug/g) < LOQ (0.0039 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.00803 ug/g) < LOQ (0.0039 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.00803 ug/g) < LOQ (0.0039 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.00803 ug/g) < LOQ (0.0039 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.00803 ug/g) < LOQ (0.0039 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.00803 ug/g) < LOQ (0.0039 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00773 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (U00773 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (U00773 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (U00773 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g)
<LOQ (0.0038
ug/g) <LOQ (0.0038
ug/g) <LOQ (0.0038
ug/g) <LOQ (0.0038
ug/g) <LOQ (0.0038
ug/g) <LOQ (0.0038
ug/g) <LOQ (0.0038
ug/g) <LOQ (0.0038
ug/g) <LOQ (0.0038
ug/g) <LOQ (0.0038
ug/g) <LOQ (0.0038
ug/g) <LOQ (0.0038
ug/g) <LOQ (0.0038
ug/g) <LOQ (0.0038
ug/g) <LOQ (0.0038
ug/g) <LOQ (0.0038
ug/g) <LOQ (0.0038
ug/g) <LOQ (0.0038
ug/g) <LOQ (0.0038
23
BACK TO MAIN
Fish Muscle Fish Muscle Fish Muscle Fish Muscle Fish Muscle Fish Muscle Fish Muscle Fish Muscle Fish Muscle Fish Muscle Fish Muscle Fish Muscle Fish Muscle Fish Muscle Fish Muscle Fish Muscle Fish Muscle Fish Muscle
11B (2000021-18) 13B (2000021-19) 14B (2000021-13) 15B (2000021-11) 16B (2000021-15) 17B (2000021-12) 20B (2000021-16) 22B (2000021-20) 25B (2000047-14) 27B (2000047-15) 29B (2000047-16) 31B (2000047-17) 32B (2000104-19) 33B (2000104-18) 34B (2000104-20) 36B (2000104-17) 38B (2000020-10) 39B (2000020-07)
Muscle Muscle Muscle Muscle Muscle Muscle Muscle Muscle Muscle Muscle Muscle Muscle Muscle Muscle Muscle Muscle Muscle Muscle
Michigan inland Lakes Michigan inland Lakes Michigan inland Lakes Michigan inland Lakes Michigan inland Lakes Michigan inland Lakes Michigan inland Lakes Michigan inland Lakes Michigan inland Lakes Michigan inland Lakes Michigan inland Lakes Michigan inland Lakes Michigan inland Lakes Michigan inland Lakes Michigan inland Lakes Michigan inland Lakes Michigan inland Lakes Michigan inland Lakes
<LOQ (0.00773 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00773 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00773 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00773 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00773 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00773 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00773 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00773 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00773 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00773 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00773 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00773 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00773 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00773 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00773 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00773 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00773 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00773 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g)
ug/g)
<LOQ (0.0038 ug/g)
<LOQ (0.0038 ug/g)
<LOQ (0.0038 ug/g)
<LOQ (0.0038 ug/g)
<LOQ (0.0038 ug/g)
<LOQ (0.0038 ug/g)
<LOQ (0.0038 ug/g)
<LOQ (0.0038 ug/g)
<LOQ (0.0038 ug/g)
<LOQ (0.0038 ug/g)
<LOQ (0.0038 ug/g)
<LOQ (0.0038 ug/g)
<LOQ (0.0038 ug/g)
<LOQ (0.0038 ug/g)
<LOQ (0.0038 ug/g)
<LOQ (0.0038 ug/g)
<LOQ (0.0038 ug/g)
<LOQ (0.0038
ug/g)
24
Fish Muscle 48B (2000020-06) Muscle Michigan inland Lakes Fish Muscle 49B (2000020-08) Muscle Michigan inland Lakes Fish Muscle 5 IB (2000020-09) Muscle Michigan inland Lakes Fish Muscle 53B (2000020-05) Muscle Michigan inland Lakes Fish Muscle 54B (2000020-04) Muscle Michigan inland Lakes Fish Muscle 56B (2000020-03) Muscle Michigan inland Lakes Fish Muscle 62B (2000020-02) Muscle Michigan inland Lakes Fish Muscle 63B (2000020-01) Muscle Michigan inland Lakes Fish Muscle 64B (20000303-01) Muscle Michigan inland Lakes Fish Muscle 73B (20000303-04) Muscle Michigan inland Lakes Fish Muscle 77B (20000302-02) Muscle Michigan inland Lakes Fish Muscle 80B (20000303-03) Muscle Michigan inland Lakes Fish Muscle 84B (20000303-02) Muscle Michigan inland Lakes Fish Muscle 89B (20000302-04) Muscle Michigan inland Lakes Fish Muscle 91B (20000303-05) Muscle Michigan inland Lakes Fish Muscle 98B (20000302-05) Muscle Michigan inland Lakes Fish Muscle 104 (200003301-02) Muscle Michigan inland Lakes Fish Muscle 110 (20000301-04) Muscle Michigan inland Lakes Fish Muscle 111 (20000302-03) Muscle Michigan inland Lakes
BACK TO MAIN
<LOQ (0.00773 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.0038 ug/g)
<LOQ (0.00773 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.0038 ug/g)
<LOQ (0.0039 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038 ug/g)
<LOQ (0.0039 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038 ug/g)
<LOQ (0.0039 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038 ug/g)
<LOQ (0.0039 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038 ug/g)
<LOQ (0.0039 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038 ug/g)
<LOQ (0.0039 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038 ug/g)
<LOQ (0.0039 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038 ug/g)
<LOQ (0.0039 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038 ug/g)
<LOQ (0.0039 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038 ug/g)
<LOQ (0.0039 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038 ug/g)
<LOQ (0.0039 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038 ug/g)
<LOQ (0.0039 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038 ug/g)
<LOQ (0.0039 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038 ug/g)
<LOQ (0.0039 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038 ug/g)
<LOQ (0.00773 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00750 ug/g)
<LOQ (0.00773 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00750 ug/g)
<LOQ (0.00773 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00750
25
BACK TO MAIN
Fish Muscle Fish Muscle Fish Muscle Fish Muscle Fish Muscle Fish Muscle Fish Muscle Fish Muscle Fish Muscle Fish Muscle Fish Muscle Fish Muscle Fish Muscle Fish Muscle Fish Muscle Fish Muscle Fish Muscle Fish Muscle
113 (20000301-05)
115 (200003301-03)
118 (200003301-01)
120 (20000301-01)
121 (2000071-01)
127 (2000071-02)
129 (2000071-03)
130 (2000071-04)
133 (2000071-05)
134 (2000071-06)
136 (2000071-07)
137 (2000071-08)
139 (2000071-09)
140 (2000071-10)
144 (1999029/F000190)
147 (1999029/F000192)
148 (199902902/F000189)
149 (1999029/F000188)
Muscle Michigan inland Lakes Muscle Michigan inland Lakes Muscle Michigan inland Lakes Muscle Michigan inland Lakes Muscle Michigan inland Lakes Muscle Michigan inland Lakes Muscle Michigan inland Lakes Muscle Michigan inland Lakes Muscle Michigan inland Lakes Muscle Michigan inland Lakes Muscle Michigan inland Lakes Muscle Michigan inland Lakes Muscle Michigan inland Lakes Muscle Michigan inland Lakes Muscle Michigan inland Lakes Muscle Michigan inland Lakes Muscle Michigan inland Lakes Muscle Michigan inland Lakes
ug/g)
<LOQ (0.00773 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00750 ug/g)
<LOQ (0.00773 ug/g) <LOQ (0.00765 ug/g) X <LOQ (0.00750 ug/g)
<LOQ (0.00773 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00750 ug/g)
<LOQ (0.00773 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00750 ug/g)
<LOQ (0.00773 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00750 ug/g)
<LOQ (0.00773 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00750 ug/g)
<LOQ (0.00773 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00750 ug/g)
<LOQ (0.00773 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00750 ug/g)
<LOQ (0.00773 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00750 ug/g)
<LOQ (0.00773 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00750 ug/g)
<LOQ (0.00773 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00750 ug/g)
<LOQ (0.00773 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00750 ug/g)
<LOQ (0.00773 ug/g) <LOQ (0.00765 ug/g) X <LOQ (0.00750 ug/g)
<LOQ (0.00773 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00750 ug/g)
<LOQ (0.00773 ug/g) <LOQ (0.00765 ug/g) X <LOQ (0.00750 ug/g)
<LOQ (0.00773 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00750 ug/g)
<LOQ (0.00773 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00750 ug/g)
<LOQ (0.00773 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00750
ug/g)
26
Fish Muscle Fish Muscle Fish Muscle Fish Muscle Fish Muscle Fish Muscle Fish Muscle Fish Muscle Fish Muscle Fish Muscle Fish Muscle Fish Muscle Fish Muscle Fish Muscle Fish Muscle Fish Muscle Fish Muscle Fish Muscle Fish Muscle
150 (199902904/F000191) 151 (1999006/2000-
5052) 152 (1999006/2000-
5054) 153 (1999006/2000-
5048) 154 (1999006/2000-
5059) 155 (1999006/2000-
5046) 156 (1999-040-
4/F00366) 157 (1999-040-
9/F00371) 158 (1999-040-
6/F00368) 159 (1999-040-
5/F00367) 160 (1999-040-
7/F00369) 163 (199900107/F000173) 164 (199900110/F000176) 165 (199900105/F000171) 166 (199900106/F000172) 167 (199900102/F000168) 168 (1999028-0610/F000333) 169(1999028-0610/F000335) 170 (1999028-
Muscle Michigan inland Lakes Muscle Michigan inland Lakes Muscle Michigan inland Lakes Muscle Michigan inland Lakes Muscle Michigan inland Lakes Muscle Michigan inland Lakes Muscle Michigan inland Lakes Muscle Michigan inland Lakes Muscle Michigan inland Lakes Muscle Michigan inland Lakes Muscle Michigan inland Lakes Muscle Michigan inland Lakes Muscle Michigan inland Lakes Muscle Michigan inland Lakes Muscle Michigan inland Lakes Muscle Michigan inland Lakes Muscle Michigan inland Lakes Muscle Michigan inland Lakes Muscle Michigan inland Lakes
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<LOQ (0.00773 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.00803 ug/g) <LOQ (0.00750 ug/g)
<LOQ (0.0039 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038 ug/g)
<LOQ (0.0039 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038 ug/g)
<LOQ (0.0039 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038 ug/g)
<LOQ (0.0039 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038 ug/g)
<LOQ (0.0039 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038 ug/g)
<LOQ (0.0039 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038 ug/g)
<LOQ (0.0039 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038 ug/g)
<LOQ (0.0039 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038 ug/g)
<LOQ (0.0039 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038 ug/g)
<LOQ (0.0039 ug/g) <LOQ (0.00765 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038 ug/g)
<LOQ (0.0039 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038 ug/g)
<LOQ (0.0039 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038 ug/g)
<LOQ (0.0039 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038 ug/g)
<LOQ (0.0039 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038 ug/g)
<LOQ (0.0039 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038 ug/g)
<LOQ (0.0039 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038 ug/g)
<LOQ (0.0039 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038 ug/g)
X <LOQ (0.0038 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038
27
Fish Muscle Fish Muscle Fish Muscle Fish Muscle Fish Muscle Fish Muscle Fish Muscle
07/F000332)
171 (1999028-0610/F000331)
172 (1999006/20005031)
173 (1999006/20005043)
174 (1999006/20005042)
175 (1999006/20005024)
176 (1999006/20005040)
179 (F960510)
Muscle Michigan inland Lakes Muscle Michigan inland Lakes Muscle Michigan inland Lakes Muscle Michigan inland Lakes Muscle Michigan inland Lakes Muscle Michigan inland Lakes Muscle Michigan inland Lakes
Fish Muscle
182 (F960513) Muscle Michigan inland Lakes
Fish Muscle
183 (F960517) Muscle Michigan inland Lakes
Fish Muscle
184 (F97012)
Muscle Michigan inland Lakes
Fish Muscle
185 (F97022)
Muscle Michigan inland Lakes
Fish Muscle
186 (F970298) Muscle Michigan inland Lakes
Fish Muscle
187 (F970301) Muscle Michigan inland Lakes
Fish Muscle
188 (F970303) Muscle Michigan inland Lakes
Fish Muscle
189 (F970304) Muscle Michigan inland Lakes
Fish Muscle
190 (F970306) Muscle Michigan inland Lakes
Fish Muscle
191 (F970308) Muscle Michigan inland Lakes
Fish Muscle
192 (F970317) Muscle Michigan inland Lakes
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x
<LOQ (0.0039 ug/g) <LOQ (0.0039 ug/g) <LOQ (0.0039 ug/g) <LOQ (0.0039 ug/g) <LOQ (0.0039 ug/g) <LOQ (0.0039 ug/g)
X <LOQ (0.0039 ug/g)
X X <LOQ (0.0039 ug/g) <LOQ (0.0039 ug/g) <LOQ (0.0039 ug/g) <LOQ (0.0039 ug/g) <LOQ (0.0039 ug/g) <LOQ (0.0039 ug/g) <LOQ (0.0039 ug/g)
<LOQ (0.0038 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.0038 ug/g) <LOQ (0.0038 ug/g)
<LOQ (0.0201 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0201 ug/g)
ug/g) <LOQ (0.0038
ug/g) <LOQ (0.0038
ug/g) <LOQ (0.0038
ug/g) <LOQ (0.0038
ug/g) <LOQ (0.0038
ug/g) <LOQ (0.0038
ug/g) <LOQ (0.0038
ug/g) <LOQ (0.0038
ug/g) <LOQ (0.0038
ug/g) <LOQ (0.0038
ug/g) <LOQ (0.0038
ug/g) <LOQ (0.0038
ug/g) <LOQ (0.0038
ug/g) <LOQ (0.0038
ug/g) <LOQ (0.0038
ug/g) <LOQ (0.0038
ug/g) <LOQ (0.0038
ug/g) <LOQ (0.0038
ug/g)
28
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Fish Muscle
195 (F990284)
Fish Muscle Fish Muscle
196 (F990286) 199 (F990287)
Fish Muscle
200 (F990294)
Fish Muscle Fish Muscle
201 (F990295) 202 (F990296)
PFOS = Perfluorooctanesulfonate PFHS = Perfluorohexanesulfonate X = Detected, not quantified
Muscle Michigan inland Lakes <LOQ (0.0039 ug/g) Muscle Michigan inland Lakes <LOQ (0.0039 ug/g) Muscle Michigan inland Lakes <LOQ (0.0039 ug/g) Muscle Michigan inland Lakes <LOQ (0.0039 ug/g) Muscle Michigan inland Lakes <LOQ (0.0039 ug/g) Muscle Michigan inland Lakes <LOQ (0.0039 ug/g)
FOSA = Perfluorooctanesulfonamide PFOA = Perfluorooctanoate
<LOQ (0.0038 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038 ug/g)
<LOQ (0.0038 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038 ug/g)
<LOQ (0.0038 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038 ug/g)
<LOQ (0.0038 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038 ug/g)
<LOQ (0.0038 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038 ug/g)
<LOQ (0.0038 ug/g) <LOQ (0.0201 ug/g) <LOQ (0.0038
ug/g) LOQ = Limit of quantitation - values in parenthesis
APPENDIX VII
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Matrix spike and matrix spike duplicate recoveries (%) of fluorochemicals spiked to fish matrices
Matrix
Chinook salmon liver Lake whitefish liver Brown trout liver Tuna liver Bluefin tuna blood Swordfish blood Antarctic fish muscle Salmon liver 1 Salmon liver 2 Fish muscle Fish muscle Fish liver Fish liver Fish muscle Fish eggs Fish testes
PFOS (Mean +/- RSD or
PRD)
230+/ -24 334+/-10 135+/-5
68 42+/-23 67+/-12 68+/-6 63+/-46 70+/-129 115+/-6 99+/-6 117+/-8 80+/-17 50+/-16 79+/-16
6+/-3
FOSA (Mean +/RSD or RPD)
47+/-26 70+/-8 82+/-3
59 131+/-29 112+/-21 79+/-6 19+/-27 71+/-34 82+/-14 66+/-0 54+/-10 81+/-24 59+/-9 92+/-12
9+/-3
PFOA (Mean +/- RSD or RPD)
121+/-17 139+/-9 124+/-6
84 48+/-8 66+/-8 101+/-8 41+/-67 76+/-27
116+/-11 103+/-4 41+/-26 60+/-22 59+/-17 64+/-18 10+/-4
PFHS (Mean +/- RSD or RPD)
114+/-4 63+/-18 83+/-4
55 52+/-3 67+/ -10 54+/-13 16+/-71 50+/-8 83+/-9 57+/-2 15+/-11 50+/-40 45+/-23 46+/-36 5+/-3
RPD= Relative percent difference; RSD= Relative standard deviation PFOS = Perfluorooctanesulfonate FOSA = Perfluorooctanesulfonamide PFOA = Perfluorooctanoate
PFHS = Perfluorohexanesulfonate
30
