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ACUTE TOXICITY TO FISH TEST SUBSTANCE Identity: rNe-fmeertrheydltpoerafsluNo-rMooecFtOanSeEsAullcfoohnoalm,idFoCe-t7h9a0n,olo;r may also be FM-3925. In one tOocxticainteyssutlufdoynaomnidbleu,egNil-lmseutnhfiyslh-,1r,e1f.er2r,e2d,t3o3a,s4,F4C-.35952.56.,68(,.187-8.-7 heptadecafluoro-N-(2-hydroxyethyl)-, CAS # 24448-09-7) Remarks: Material is an off-white, waxy solid. Sample purity and lot number were not recorded. METHOD `Summary of 2 Studies TMyepteh:odS:tatUi.cS.acEuPteA 660/3-75-009, April, 1975. GLP: Year cNoompleted: 1978 Species: Lepomis macrochirus ASnuaplpyltiiecra:lDmaolneitFaotrtiingg:FishNoHmaitncahlercyo,ncBernatdrya,tNioenbsraonslkya. Temperature, pH and dissolved oxygen recorded CEaxrproiesru:reAcpeetrioonde:--960-.h5omuLrsper liter test solution Statistical methods: LCs; values were not calculated because the highest concentration tested induced less than 50% mortality. Concentrations tested exceed water solubility of the test substance. Test fish age: Not noted. Length and weight: Average length = 4.0 cm Average weight =0.5g PLroeatdrienagt:me0n.6t3: gNfoinshe/L Test coDnidliuttiioonnsw:ater: Carbon-filtered well water Dilution water chemistry: Hardness": 236 mg/L as CaCOs cDoOp:*: 8<1.9mmgg//LL Alkalinity*: 221 mg/L as CaCO3 CpHo:nductivity: Total Solids*: 47290 pmhos/om 286 mg/L 006594 + Values taken as averages from general laboratory water quality testing in 1978. Sditsoscoklvainngdktneostwnsowleuitgihotnsporfeptaheratteisotns:uTbessttanscoeluitnio8nsmpLreapcaerteodneby cboenfcoernetmriaxtiinognowfit0h.5dilmuitipoenrwalitteerr.of Tdhiliustiroen swtateedr.inTahne atecsettone tshuebstetsat.nceItwwaass ngoetneerdatlolyprneoctiepidtattheatoumtorgerapdrueaclilpyitoavteerftohremecdouirnstehoef Chiognhceernctornacteinotnrastdioonsitnesgt rsaotleu:tiOonnsc.e SEtxapbiolsiutyroefvetshseetless:t 3c5hxe2mi0cxa2l0csmol(u5tigoanlsl:on)Prgelcaispistaaqteuanroitaed cNounmtabienirngof1r6eLploifctaetsets:soolunteion. Number Number of of fish per replicate: twenty concentrations: Five plus a solvent control and a negative control Water chemistry Dissolved during the study: oxygen range (24 -- 96 hours): 35..74--65..18mmggllLL((ccoonnttrroolleexxppoossuurreeTTeesstt12)) 5.0-6.0 mg/L 3.4-52mglL (100 (320 mg/L mglL exposure exposure Test Test 1) 2) pH range (24 -- 8.2-8.2 96 hours) (control exposure Test 1) 7.87.9 7.8-7.9 (controlexposureTest 2) (100 mg/L exposure Test 1) 7.8-7.9 (320 mg/L exposure Test 2) Test temperature range (0 -- 96 hours): Remarks: _ 17-20C Test fish were randomly allocated to each test tcehmapmebraetruwriet,hidnis3s0olmvienduotexsygfeonllolweivneglstaoxnidcapnHt aodfdialtliotne.st Msoorltuatliiotnys,. were measured at 24-hour intervals `sAufffirisctiesnttumdoyrwtaalsitcyotnodaulcltoewdthate ccoalnccuelnattriaotnioofnsantoLoCl5o0w.toThinedsutcuedy wseacsornedpestautdeyd, aftishhimgohretraltietsyt wsuabsstoabnsceervceodncteontbreatlioownes.r thIannthien the ifinrsstolsutbuldye.naTthuirsenoofn-trheeptreosdtuscuibbslteamnocret.ality was probably due to the 006595 RESULTS Nominal concentrations: Test1: One blank and one 0.5 milL solvent control, 13, 22, Test2: 36, 60, 100 mg/L One blank and one 0.5 milL solvent control, 4 test concentrations that are and 180 mg/L and 320 not legible on the test mg/L test solutions. sheet, Elementvalue: 96-hour LCs: Not calculated for either test. Exceeds solubility. Test 1 Percent Mortality CoTnocmaanatTono. atous| 724s [Feg 0 | ca 0[ w0o1r0| ] [Eoveeama | 0 @ 7TE [wm [oTorsrE [= [vo [vee [ [Cw e [ oo [v o er e| CmT7117 =T1T3] Test 2 Percent Mortality ConNcoarnraaiToenst, Abtous | T2tous [Sov= emCarea| r| = 5 [ TW 5T [+R 17] R%S || [ [ooo] [eo [oo ows [oe 5[wo ww) C=1s1&751%] CONCLUSIONS TdehteerNm-imneetdhytlo FbOe SgrEeaatlecrohtohlan96t-hheotuerstLCssuobfsotranblcueegsiolllubsiulniftiyslhimwita. s 006596 Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Ruesliinagbitlhietym:etKhioidmioslcohgyraanckcienpgt3a.bleThatestheeisrtutdiimees,,lwahciklaenawleyltliccaolndduatcatefdor ddeetteerrmmiinnaattiioonnoofftthhee tseasmtpsluebspturaintcy.e cToenscteinntgrwataisonalisnotdheonteestweslolluatbioovnse atnhde solubility limit of the test substance with the aid of acetone to enhance saollluobwilaibtlye. Tmhaexisomluvmentofc1on0t%r.ol Tien stthiengseschoonudldtehsatvweabsegernearteeplritchataend.the REFERENCES These studies Laboratory, 3 were Lab cRoenqduuecsttendubmybtehre431M97C,o1m9p7a6n.y, Environmental OTHER Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 Last changed: 5/17/00 006597