Document mm54p9d6XZonwZDm2ap0XK2xB
HAZLOTON
LABORATORIES
AN4ERICA. INC.
3301 KINSMAN BLVD. P.O.BOX 7545 MADISON, WISCONSIN 63707
Chemical& B!oMedicalSciencesDivision
PHONE (6082)41-4471 TLX 703966HAZRAL MDS UD
FINAL REPORT
JAN 2 8 1988
RNGFP G. PFPKINS
MINNESOTA
MINING &
TOXICOLOGY SERUICES
ST. PAI-,IL.7MN c55101
MANUFACTURING
COMPANY
SAMPLE:
T-4102
Pt.]RCHAqF ORDER NIJMBFR: T837389-410 754
SAMPLE NUMBER: SAMPLE ENTERED:
REPORT PRINTED:
70905761
09/25/87
01/215/8R
FNCLOSFD:
ACUTE ORAL TOXICITY
STUDY IN RATS (OECD GUIDELIt,4ES)
0 Key Personnel
0 Method Summary Individual
Patholoov
References
Pathology
Report
RaiA Data Appendix
Comments
SIGNED:
STEUFN STUDY ACUTE
M. GL,.AZA DIRECTOR TOXICOLOGY
........ DATE
.%.4z.@k...........
HAZLOZON
LAEK3RATCRlr=S AFVERICA. INC.
3301 KINSMAN BLVD. 0 P.O.BOX 7545 MADISON, WISCONSIN 53707
Chemical& B!oMedicalSciencesDivision
PHONE (6082)41-4471- TLX 703956HAZRAL MDS UD
SAMPLE NLIMBER: 70905761
C;AMPLE: T-4102
PAT@IE 2
KEY PERSONNEI-
Acute Toxicoloo!2
Steven M. Glaza Stud@) Director
Calvin L. Horton Group Leader Skipport Services
Sharen Report
L. Howery Coardinaior
n@i.jalit@2 Assurance
Debra Cijrle5i Arndt Manager
Anatomical
Patholoqk.)
Thoma5 E. Palmer, PhD
Anatomical
Pathologist
Robert Senior
Sala,,)a Section
Supervisor
Dennis Hof@man Group Leader NecrOP55)
Anne Mosher Group Leader Patholog@) Data
t-aboratorki Animal Ueterinarian
Cindk) J. Cary, D@JM
Diplomate,
ACL.AM
HAZLEZON
LABORATC)RIES ANAERICA, INC.
Chemical& B!oMedicalSciencesDivision
3301 KINSMAN BLVD. 9 P.O.BOX 7545 a MADISON, WISCONSIN 53707 * PHONE (608)241-44719 TLX 703956HAZRAL MDS UD
I:n7AMPI-F @41.IMRFP: 71l9O'576l
C;AMPLF: T-4102
PAGE
3
OECD ORAT- SCREEN
Ob iP-r,,t i-,,,e To determine
the acute oral toxicit%) produced when a test
material is administered
by the oral route (gavage) to rat5 accordina to
the Orcani5ation
for Economic Cooperation
and Dek)elopment'5
for Testino of Chemicals.
Test Material:
T-4102
Ph,,)5ical Description:
Pi.iritt,a)nd Stabilit@):
Storage and Retention:
'-;;gff--tPkr.ejcautions:
Dark amber liquid Sponsor a55ume5 re5nonsibilit@,)
for
purity and stability determination5. The test material was stared at ronm
Any unu5ed material will be di5carded
to HL.A Standard Normal handling
Operating procedures
Procedure. were used
to HLA Standard Operating
Procedure.
temperature. accordiriq
according
Test Animal:
YOLing adult albino rat5 of the '-;prague-Dawle5i 5trai.n @-jere.
procured,
separated
b%) 5ex, maintained
in group canes in temperature-
and humidity-controlled
quarters, provided continuous access to Purina
Rodent Choij and water" and held for an acclimation
period of at lea5t
7 dakis. Animal husbandry and housing at HLA complkj with standards
olitlined in the-."rijido,-for the Care and Use of Lab@ratork.) Animals".
li: variations
from the prescribed
environmental
conditions existed.,
thp.-,jwere documented
and con5idered
to have no affect on the stud,,j
oijtccirti(-.N.o contaminant5
were expected to have been Dresent in tkc-.
feed or water i.ihich would hak,,e interfered with or aff@-cted the results
of the study.
Acclimated
animals were chosen at random for the stud,,). Test animals
k@tere housed by sex in orOUP5
Of five and identified by animal number
and corresponding
ear tag. Food and Ljater were available ad libiti-im
the study, exceot for an overnight period just before
test material ailmini5trati@nwhen food, but not tiaterl, ijas withheld.
F'easnn for Species Selection:
The rat is the animal cla5SiC811.5)
used due to
its small siz#.-; readk) a%.,ailability, and large amount of background
data.
Method:
Fik,,e male and five
used for each dose level.
(0.150 and 5.0 9/kg).
female rat5 i.ieiighing from 200 to 284 g were The study consisted of ti.indose lek,'F--IS
Preraration
and Administration
of Test
i.ia-@calrulgtc-.d for each animal based
and administered
i.indili-itedbk)
tlaterial,: An individual dr.),5e upon its fasted bcidv wein@-it
The
riri.-@,e.-olijrnoef the test material
,)aried per
bi.ilk rlF--nsit@,o)f i.n6 q/mi.
do--,e lekdel-based
upon
WHAZLEZON
LAE3C3=.ATX3RIES ANAERICA, lP4C.
Chemical& BioMedicaSlciencesDivision
3301 KINSMAN BLVD. o P.O.BOX 7545 - MADISON, WISCONSIN 53707 o PHONE (608)241-4471- TLX 703956HAZRAL MOS UD
c@AMPt-F NI..I@IRFR: 70905761 '.;AMP[.-ET:-4102
P A -rE, 4
OECD ORAL SCREEN
(CONTINIJED)
Reason for Route of Administrati.on:
a knoi-inqijantit@)of test substance historically.
This is the method for administerino and has bt-en the roijtf-o-f choice
Oh5ervation5: The animals were observed for clinical 5inns and mortality at ly 2.5 and 4 hours after test material administrati;n. The animals i@it-rF--observed daily thereafto--rfor 14 dak)s for clinical sirn- and ti.iice a da5) for mortal ity.
All animals were iif--igheidu-stbefore test material ariministration,, at 7 Ha5is and at study termination (or at death).
Patholoo5): At 5ttidI-,tjermination surviving animals were euthanatized. Animals which died an study or euthanatized at study termi.nation were .Sljbjected to a gross necropsy examination and abnormalities recorded. Folln4jing necrop@;-I ,),animals were discarded and no tissues ilere 5awed.
'-;tati-stiralMr-.thnds: Other than average body weights, no other statistical method i.tasperformed.
L.ncation of Rat@iData and Final Report; The raw data and a copkj of the final re.rort will be retained in the archives of HLA.
HAZLETON
LQBC)F;A70RIES ANLERICA, INC-
Chemical& BioMedicalSciencesDivision
3301 KINSMAN BLVD. o P.O.BOX 7545 MADISON, WISCONSIN 53707 # PHONE (6082)41-4471o TLX 703966HAZRAL MDS UD
SA@IPI.-E NIIMRFR: 7n9O'.:7t;l
SAtlPL@E: T-41 02
PAF-,E
OFCD ORAI- SCREFN
(CONTINUED)
SUMMARY
Test Animal: Albino Rats
SpragLIe-Dat@lle@) strain
Source:
Charle5 River
Laboratories,
Inc.,
Portaoe
Mi
Date Animals Recei- ved:
09/08.P 10/12@ and 10/26/87
Method of Administrati.on:
Oral Gavage
Date Test Started:
10/26/87
Date Test Completed:
ll./2n/e7
E-7,tin-iatF-cOlral
I-D'50:
MAle - Between 0.130 anr.1 5.0 9/kq of boriI,,jLjF--ight. Female - Between 0.50 and 5.0 9/kg of body ujeight
Dose Lek@el (9/k'3)
m,gle
n.50 5.00
Fp.ma I F-
n .rgn
5.no
Akierage Initial
272 2'5r,
210 216
Bodk)I Ldeight5 (9)
Day 7
Terminal
273 ---
339 ---
198 ---
238 ---
Mortal,it,,I) (Ni-irrobF--r
Dead/Number
Dosed)
2/c3 13/5
0/5 5/c3
HAZLETON
LABCX4ATORIES ANAERICA, INC.
Chemical& BioMedicaSlciencesDivision
3301 KINSMAN BLVD. a P.O.BOX 7545 o MADISON, WISCONSIN 53707 o PHONE (608)241-4471a TLX 703956HAZRAL MDS UD
@;AMPI..F NIJMBFP: /-090'F,761
SArIPI--E: T-41-02
PAGE
6
OFCD ORAL SCPFFN
(CONTINI,JED)
(No.
CLINICAL
SIGNS
of Animals Affected)
Hours 1.0 2.5
4.0
Da@25 1 2 3 4 5 6 7 8 9 30 11 12 13 14
nnSE I-Fk)EL: 0.50 9/kg of body weight
Males
Anpeared normal 1.5 5
5
555400333 3 3 3 3 3
Anor-es5ive
'@ehak.,ior
0
0
0
0 0 0 3A 1 1 0 0 0 0 0 n 0 0
Hkjnersen5itivity
to touch
0
0
0
0 0 0 0 12 000
0
0
0
0
0
H1k1,iriic.t iv it,1,)
0
ntermittf.-nt r-I,on ic
0
000021000 0 0 0 0 0
I-ori-I1F),[iion5
0
0
0
0 0 0 0 0 1A 0 0 0
0
0
0
ri
0
DF-.ath
0
0
0
0 0 0 2B 0 0* 0 0 0 0 0 0 0 0
Females
Appe.arF,-d normal 13 5
5
5 5 13 0 0 0 3 3 3
4
4
4
4
4
Aoore.ssive @ehavior
H-,Jnciacti-,,tjki)
0
0
0
00 054 0000 0 0 0 0 0
0
a
0
0000 11000 0 0 0 n 0
Hkljpf-rsf-.ints ik,,tiy
to tn-ijr-h Ataxia
0
0
0
00 0024 1 1 1 0 0 0 0 0
0
0
0
00 0011000 0 0 0 0 0
IntF--rmittent clonic
r,nnwij1.5ions
0
0
0
0 0002 000 0 0 0 0 0 0
All-)Deria b@r--k region
0
0
0
0 0 0 0 1 1 1 1 1 1 1. 1 1 1
A - I.;irin n n t F--d af ter a .m. observation. 8 - I-Ineanimal found dead after a.m. observation.
HAZLMZCN
LAECF;ATC)RIES ANERICA, INC.
Chemical& B!oMedicalSciencesDivision
3301 KINSMAN BLVD. * P.O.BOX 7545 0 MADISON, WISCONSIN 53707 - PHONE (608)241-44719 TLX 703956HAZRAL MDS UD
NI-JMRFR: 7090E,;761 ,@;AMPLF: T-4102
PAGE
7
OFCD ORAL SCREEN
(COt4TINtJED)
CLINICAL SIGNS (Continued)
(No. of Animals Affected)
Hnur--,
1.0 2.5 4.0
Daus
1 2 3 4 5 6 7 8 9 10 3.1 12 13 14
F)NSF L-Fk)F[-: 5.0 f3/kn of body i.;eig Fit
Males
rlinsis
4
0
0
Ata-)Cia
2
1
0
Ppd-stained face
L-arrimation
0
0
0
0
0
n
Yellai4-stained
genital region 0
0
0
Pain reflex
ah5ent
0
0
0
Diarrhea
0
0
0
Tonir--
cr.)n-,)@il5ions 0
0
0
Fxcessi,,ie
5ali,,,,ation
Cie-.ath
0
0
0
n 00
A opeared normal 0
0
0
H@@pnatli,7i,t,
5
5
5
1
2
1
II
ta inc-d
genital region 0
0
0
f-,aspi nn
0
0
n
Red-5t.ained
fac,c-.
0
0
0
Pp--,piratorki
conot-.--ti@n
n iarr@pa
0
0
000
Tnn it-
r-,rinI,-,,)iio-ni-,
0
0
0
F)Fa- t h
0
0
0
5 2 0 0 ----0 0 0 a ----0 0 1 0 -----
3100 2 0 0 0 -----
0 1 0 0 ----- - - - -
0 1 0 0 ----- - - - 0 0 1 0 ----- - - - -
0 0 1 0 -----
0 0 1 0 ---- -
1A 2 1 1 - - - - -
Females
2 0 0 0 0 0 --1 0 1 0 0 0 --0 0 0 0 0 0 --1 0 1 0 0 0 ---
2 1 2 2 2 0 ---
1 0 0 a 0 a ---
--11 100a
--100000
0 1, 0 0 0 0 - - -
0 0 1 0 2 0 --1A 1 0 1 IA I - - -
A - Found dead at p.m. mortalitki check.
HAZLEZON
LABORArDRiEs
AIV*RICA, INC.
Chemical& BioMedicaSlcienceDsivision
3301 KINSMAN BLVD. & P.O.BOX 7645 a MADISON, WISCONSIN 53707 9 PHONE (608)241-4471* TLX 703956HAZRAL MDS UD
SAMPI.-.NFL-IMBER: 709013761 SAMPL.F: T-4102
OFCD ORAL SCREEN
(CONTINUED)
PATHOLOGY
DOSF LEk)Et-: 0.50 9/kg of body weioht
An i rriaI Nijmhf-r
Test Day
spx
Died Sacrificed
Cn308l
m
4
Cf.13080
m
4
-
cn3082
m
-
14
Necrop5y
Comment5
No visible lesions.
Ventral cervical region and
both mandibles
cannibalized.
No visible lesions.
C03079
m
Cn3088
m
-
14
-
14
No visible lesions. No visible le5ions.
C03?49
F
-
14
No visible lesions.
r-n3247
F
-
14
No visible lesion5.
r.n32150
F
-
14
Nn visible lesions.
cn3?48
F
-
14
No visible le5ions.
C03326
F
-
14
No visible le5ions.
PAI-,E a
RMIHAZLOZON
LAEK3RArORIES AWERICA, INC.
Chemical& BioMedicalSciencesDivision
3301 KINSMAN BLVD. o P.O.BOX 7545 9 MADISON, WISCONSIN 53707 9 PHONE (6082)41-4471a TLX 703956HAZRAL MDS UD
@;AMPI..F NI.-IMBER: 709015761 @@;A@lPt.F: T-4ln2
pAfl-E
9
OFCD ORAL SCREEN
(CONTINUED)
PATH13LOGY
(continued)
DOSF [-EUEI,-: 15.0 a/ki-3 of body i@ieight
Animal Ni.imber
Sc-.y
Te@t Da@) Di.ed Sacri.Ficed
NecropsQ
Comments
C110499
m
4
Perineum
stains
-
olandular
oortion
@ocal area'5 (1 mm
tan
stomach
-
has dark brown
in diameter).
COOIF,04
m
3
Perineum/perianal
nasal
discharge
crusted.
stain.-, - dark. red @nd
C00503
m
2
r-nnF,oi- m
I
-
cnn,562
Pi
2
-
(70303r7
F
2
-
Cn3ll34
F
4
-
f7ll3o32
F
6
-
Nasal
and mouth
broi4n;
stomach
has dark brown
1 mm).
areas
- stai.ned
- alandular
oortion
ar;a5
(IJP tD'5 X
Stomach - glandular
pnrtion has
multiple,
dark brown, pinpoint
foci.
Stomach - glandular
portion has
dark. brown areas (up to 2 x 1 mm).
No vi-sibl.e le-sion,.5.
Stomach - glandular
portion has
dark brown areas (up to 3 x 1 mm).
Stomach - glandular
mijcosa has
dark brown areas (up to 4 x 1 rfim).
C03029
F
C07,1138
F
I
-
5
-
No Vi5ible lesion5.
Perineum paranasal
stains - brolin:
discharge
- red.
aI nhIHAZLEMON
47101/
3301 KINSMAN
LAEK3Pk=URIES
AA/ERICA,
INC.
BLVD. a P.O. BOX 7545 o MADISON,
WISCONSIN
Chemical & BioMedical Sciences
53707 o PHONE
(608) 241-4471
* TLX 703956 HAZRAL
Division
MDS UD
SAMPI.-F-
Nl,.IMR-ER:
c;A@1PLF: T-4102
711905761
PAGE
3,n
OFCD ORAL SCREEN
(CONTINIJED)
ReferenCe5:
I.. Or-gani-5ation for Economic
for Testina of Chemicals, adopted Ma5) 12, 1981.
Coop#--ration and Dr-velopment's Giiideline5
Section 401, Acute Oral Toxicit5i,
2. OECD's Principles of Good I-aborator,-jPractice, Annex 2; C(81)30
F in a I
3. NTH Pijblication No. 86-23 (re-,.)ise1d985).
HLA LAB NO. 70905761 PATHOLOGY REPORT Acute Oral Toxicity
PAGE 11
Ten rats (five males, five females) from each of two dose levels (0.5 and 5.0 g/kg) were necropsied. The animals that died on test were refrigerated and necropsied within 24 hours, usually the same day. All surviving animals were euthanatized and necropsied at the termination of the study. The dose level, day of death, and gross observations recorded for each animal are on pages 8 and 9 of this report.
The most frequently recorded observations were in those animals that died on test. The mucosa of the stomacn (glandular portion) of several animals had dark brown areas of variable size which were possibly treatment related. All other observations were considered related to postmortem change or incidental findings which were not related to treatment.
Thom'argE. Palmer, Pathologist
(1734mcs)
'Date
HLA No. -1(9) (oI
Personnel Signature Sheet Acute Toxicology
Name
Job Title
Signature
Becky Beckwith
Sr. Lab. Animal Asst.
Steve Beloungy
Lab.'Animal Asst.
Ken Bridges Pat Crary
Sr. Lab. Animal Asst. Sr. Lab. Animal Asst.
Shane Eith
Lab Animal Asst.
Shawn Frazier
Lab Animal Asst.
Steven M. Glaza
Group Leader
Molly Hahn
Admin. Clerk
Kevin Hamilton
Lab. Animal Asst.
Jeff Hicks
Sr. Lab. Animal Asst.
Calvin Horton
Group Leader
Sharen L. Howery AdministrativeAsst.
Gregory Johnson
Lab. Animal Tech.
Paul Krebs
Lab. Animal Asst.
Wayne ftdison
Section Supervisor
Scott McConnell Lab. Animal Tech.
Shelley 14cConnell Lab. Animl Tech.
Dawn Conant
Sr. Lab. Animal'Asst.
Don *avis
Lab. Animal Asst.
Robin Olson
Lab. Animal Asst.
Patricia Padgham Joseph J. Daun
Tom Leader Lab. Animal Asst.
Michael Patzka
Lab. Animal Asst.
John Paulson
Sr. Lab..AnimalAsst.
Jane Polnow
Lab. Animal Tech.
Dennis B. Steiner Lab. Animal Tech.
Michael Thesing
Lab. Animal Asst.
Paula 6._Vangen Albert Olson
AdministrativeAsst. Manpower
Jim Jirschele Ben Haley
LTE Sr. Lab Animal Asst.
c@)O@
--.Wj
a ilill -/2a J.
Initials -i2A-sa
igg@PXk@fill.
74A
HLA:
76
DOSE AI*IIHISTRATIOM/000Y WEIGIIT/"ORIAIITY RECORD
Study litlel Acute Oral Toxicity
lostMaterial:-F- L+ IC)z
Velilclp: NA
Bulk Density: 1.0(@
DoseLevel: O.S7C)
IgImL) SpecJos/Stralei: Albino Rat/Si)raguo-DAW!U Soisrce: CloariesRivor Date ioceivelli.@@-7
(9/kg) fasted Date/Time/Technician 1 lis7i@7/3.-So P.,.
Room flo.
ItOutoof AdministratIon. Oral Gavage
p@!e Voluime 0.14 -7fid/kgl
Sex: Male .0mal
An liimI No.
Co -1-),Rl
-IL-IBC@>o7q
Prefaited Body Weight-UL-
I)osoTImwo
3cf@?
It 154--
fasted Oody Weiglitlg) Actual Dose fit) !LAY7 Body weIoitl (g) Pfy-L4@go-dyWeiglit(g) Dead. Body__Relgiot(g)
2-4'K
IIZ. 0. 1
nA
z
?-g3
0. 1?2 0,13 0.13
(0
3,40
7
------
leclinlc14"
Data
Sca 1@ Usedi-.7-/U)
MA
6(4-
Y lfiedby:
/7 itl,3,
1
Lois--Loluoe 10,9] (W.IkQL&qlpa! no.
eU Body weiglit
Sext 11 1 a/ F-8*-ar-e'.
@zs-o
Dose T too:.
Fisted Bodyteiglittg)
Ar.tU!D,oLseItj-
is) LJL#mIoL-LdWeiglit Pty -L4Agh-lo-ijh-tJg)
!Lea-4
2AOC) 101
10 0.1 -.0.lo O.oq@q.-Oq
q e- 12Z0,331
level !gLkgt.-se-x
o-l-
mr--mi W-"i %L
NF-1-n Am
unorai,YW
6 riiMmi I
NO. oir /NO. DO ED) t ii-i-iIiF&
-7 Ni -At mr-PH
IY6
D/j
tech.
Date
1V@ 1 lf7
IIV ifid!7/0%, ITZ
NA - N(iAtpplicable - Dosage calculated, but not administered unused animal returned to stock
IT3 IT@
Reviewed
/EE(S
Tochniciall-
1z
AH
ScaleUsedrilto NA
4"0Ay&,,
--14-- -ToTaT _AA MA MAI
Dose level:
Animal Number
0-o
(@i;@Female
Observations ADDeared Normal
HLA:
OBSERVATIONS (Individual)
Test material:
L410 2-
Pre-
Hours I
Stud Day
dose 1P2 2.55 4 1 2113 4 5 6 1 7 8 9 10 111 12 13 14
Appeared Normal
CIO ct5aty-essive6eAavior-
io -6j(
DDeare Normal
Appeared Normal QA!&r4e<sive 6-einavloy, CIO
Appeared Normal C
@eaths ollectedby
vo,I'V
L/
L7 vi,
Surviv
submit necrop Techni
Date
L/ V je.@-
I.Io/ L@@
v - - - I- - - -
-IN/ -
-- -
/o)r-)t 0 U 10 n. o 0 0 0 14) 0
14
fe r q tn " e
Surviv
design and di Tpchni
Date
v Ind exi
si Sli Con Fou
A411A)] Review Date:
Bose level:
Animal Number
MaleX-e;i@le@
Observations Appeared Normal
OBSERVATIONS (Individual)
Test material: -T - L4Ic>7-
HLA:-IO
Pre-
Hours
dose 1 2.5 4
Stud Day 1 2,1 3 1 4 r55 61 71 8 9 lioI 11 12 13 114
(@@ekk-"Ae"
Appeared Normal fssive
=-,7
tkovt%L C.0 UL4,01's
117"'-77
Appeared Norml-
Af--,Vouch
_j4
cv
L LIT -
Deared Normal !Slrcssliove-@aviovc-b4@L
Appeared Normal
c Aoy-
7-7
kv
-
eaths ollected by iate llig-7
ol -T,,e--rrec--t
h::::;fp
L/
V*T-
I
-"I;Z
Surviv
submit necrop Techni
Date
Surviv
design anddi Tpchni
Date
Ind exi si Sli Con Fou AJ4 -A&) Review Date:
DOSE ADHIMISTRATIOM/OODY WEIGIIT/MORTALITY RECORD
H LA:
Study Titles Acute Oral ToxlcitX
Test Material: -T-4tGa-
Vallicipt
Bulk Density. 1-1)1. (SIML) Species/Stralit:Albino Rat/Si)rague-Dowley6ource. CitariesRiver Date ioceived:.i/?,
Do$*Level: S,O.
9/kg) fasted
Route of AdministratIon. Oral Galage
6/.-tf/r7r
P.m.A/Z RooImla.
oqqq o.q.lx
!Lole--Lolum gx#4-lmi/kg)-
Sax:
Animal No.
a] owls
liasslliw lilts
05,03C05,1Co)-54,
Teclinician
IV67
Data
Scale Ds@dKTAco
MA
Pre(asted Body Weight-ULfasted Hody Molglit Actual I)ose(mL) !LaxI Body Weight (g)
z.-742L?-Z(oOLZa.-2-'5-1
Oeaj
>
Yerifle4 by:
7;tl
4
P-axji.OodyWeig
Dead Body Weigit g)
q.7.).
Loll--Lo -0' Animal No.
. -2-3qZLIO1Z3?=] Z.1 123'Zi
Sex$ lialao4ma@
pose T Igo 11:10tlm leclinlcI&n
/*7
Date
3035' 303 o3 7-307,43303
1361
Scale Used@l?bA MA
PrefastedBodv Welgilt(g)
fasted Body Weigil 12)
ActualDose (ioL) )Liy..-L P!dL We Igilt (g) Yiy-Lt Body Weight 12) Dead Vodv Weight 21
2-t@, 2-2-0 Zz-1
1.0
1,0
1.0
0 tVrTA@i@F W
)15
zi 1
pwri
-110-2
1314
1o/.? I.
15c1>47
Teri
OC
llose
liours
level
CF---4
2
!2LkiL !Lax-
IS-o
0
AM Mon
NORTALITV NO. PIE /NO. DO ED)
ud ay
4
0.
NL;;;Rj
01 Ys tiA
z
ML
1
14 lot&l
mi PH
NNAAIR -30- MLA
lech
u to
lov -L
MAI -7-
MA
NA - Not Applicable - Dosage calculated. but not administered unu$04 Animal returned to stock
Reviewed I
Patel -"I
Dose level: S.C)q
a]lNo
A nimal
Numb r
erxaligni
peo@L eAa4red
019
&E;L= C-0&%v loasie,et
kppeared Normal
C).50
b,5(j3
4ed 4z@ee-
Appeared Norm]
4P
kopeared Norm] h
Predose
OBSERVATIONS (Individual) Test material:
lfours 2.5 1 4 -17T
Studv Dal,
34 56 7 6
10 ]FI-I1-2
7=
7;;r
All,
7- %7
--IF-
eaths
loll
b@
ate
1 2&,
lb
HLA:
Surviv
submit necrop Techni Date
surviv design and dii Technic Date
v Indi exis
Si Slig Cond foun
/vn-Aeo Reviewe Date:
Dose level:
Animal Number
Observations Appeared Normal
OBSERVATIONS (Individual) Test material: -T-41)OJL
P@Pror@see--
Hours
Stud Dav
dose 1 12.5 4 1 1 2 1 3 1 4 15 1 6 7 1 8 19 10 11 12 13 14
L/
HLA: -1@
Appeared Norm]
v
3634
APP64r6d Norm]
Co.-
4%Q
0 Y\ \J %At 0 rl
Appeared Normal
A
lLou2 ;
12ed Appeared Normal
30 31@'
,ioAiL-
)eaths ollected by
ate
iA
- = @- -
199ii V.44f'/*
1.- L 2A4-
-7
All,
I ItyllI
kfi
Surviv submit necrop Techni
Date
Survivi
designa
and dis
I
Technic
Z:7 Date
Indi exis Sl Slig Cond foun
Alty -AID
Reviewe
Date: