Document mkQEkNNmDQ8XxE1N8DoNDM8O

MICROBICS MICROTOX@) TOXICRTY TEST TEST SUBSTANCE Identity:Perfluorooctanoiaccid,ammonium salt;may alsobe referredto as PFOA ammonium salt,Ammonium perfluorooctanoateP,FO, FC-1 16, FC-1 26, FC-1 69, FC-1 43 or as the major component of FC-1015 or FC-1 015-X. (Octanoicacid,pentadecafluoro-, ammonium salt,CAS # 3825-26-1) Remarks: The testsample isFC-1015-X. It'psuritywas not sufficiently characterizedt,hough currentinformationindicatesisa 30% straight carbon chainversionofFC-143 in80% water. The 3M productlotnumber was not noted. The followingsummary applies to the testsample as a mixture of the testsubstance in water solutfonwith incompletely characterized concentrations of impurities.Data may not accuratelyrelatetoxicity of the testsample with thatof the testsubstance. Dati3was used to compare toxicityof the branchedlstraightchain ammonium pertluorooctanoatehomologue mixture in FC-143 vs.FC-1015-X. METHOD: Method: MicrobicsMicrotox@)"BASIC" Procedure Test type: Staticacute GLP: No Year Completed: 1996 Species: Photobacteriumphosphoreum Analyticalmonitoring:pH and osmoticcondition. Exposure period:30-minutes Statisticamlethods: EC50 valuescalculatebdy statisticlailnear regressionprogram providedforMicrotox@) Test organism source: Microbics'Reagent. Test conditions: Diluent:MilliporMeilli-Q"wmater Test temperature: Not noted. Stock and testsolutionpreparation: A primary6.660 g/Lstock solution was prepared inMilliporMeilli-QTwmater. The pH ofthe stocksolution was 6.9and an osmotic adjustmentwas made using200 mg NaCl dissolvedin10 mL ofstocksolution.Appearance oftestsolutionswas noted as "clearand colorless"A.lltestsolutionswere made by proportionadlilution. Exposure vessels:3 mL Cuveftes. Number of replicates:two Number of concentrations: Four plusa blankcontrol Element basis:Percentlightloss. RESULTS Nominal concentrations:Blankcontrol4,16, 832,1665, and 3330 mg/L Element value: 5-minuteEC50 = 2300 (2070 -2560) mg/L 15-minuteEC50 = 1960 (1730 -2210) mg/L 30-minuteEC50 = 1950 (1760 -2160) mg/L Element valuesbased on nominal concentrations. Remarks: Testingwas conducted on the mixtureofthe testsubstance as describedinthe testsubstance remarksfield.The values reportedapply tothatmixtureand -notthe testsubstance. CONCLUSIONS The FC-1015-X 30-minute EC50 forPhotobacteriumphosphoreum was determined to be 1950 mg/L witha 95% Confidence Intervaolf 1760 to 2160 mg/L. Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY Reliability:Klimischranking2. Testingmeets allcriterifaorquality testingb,ut lacksanalyticaclonfirmationoftestsubstance concentrations. There isa lackofcharacterizatioonfthetestsample. REFERENCES This studywas conducted by the 3M Company, Environmental Laboratory,St.Paul,MN, Lab Request number Pl 626. OTHER Last changed: 5/25/00 ENVIRONLMENTAL L TESTSUBSTAN LABORATORY:'BUCROTOX PREP SHEEr... Iz DaWAnalyst m ictz 1. PreparationofStock Solution MwvVolT.eit tance:.,,. too -p '*FiDnialutVioohn@n4@.;,@: Dituent FinCaolncenwaotfSitoonScokiuti6i@@, j 2.pH Adjustment of StockSolution, s,rnitialpHofStockSoluti@n:.'.@ *pHAdjustedto: pH Adjustedulth: 3.Osmotic Adjustment ofSt OSMORC AAJUS CO'" @v'=. c@ ofpH and OsmmuotuliaUvtd-jctiiisitdocksaindI Pjnr 1,lk 'IlCkOTOX DATA REPORI RIsic I*est P, I01!5-,Y-(RM-55192) :-Teot Ti(66 5 '56/'.7'9-. 19 6(3.:60 -A--6-4. ll,.64.19 52. -)4 974 i't;6,2 CONR. ---------- i:p0,. 416.2500 8-37 500c, t1 N ac @e'a CR/GAM mA @.0.8462, -0. 1*9' 2 74 1 -+3 4-: or tatlr@Li t Llc)r),5 T C, -.4@'@:"(15 -,@@T MF, IT 4. Fli-E -6 Fc, T 7 192) BasJ c, Test' -- ----r-i-itnro 1 C:c7ntral 14 7 ----------- -95-66/ 74.@St 93. r4i3/ '74.06 94.75/' 5,t'@ 7/ -7. 9 51 / F39. 21 / Ba. 4 86.97/ E",5.99/ t; 69 - 42.0 0 B.s COCORRECTION 0 U--Cd -iwr-c FACTIJF( C@'Ad !Lt%tMen tinacl c@o Las-i 7914 0 ocio 280, 34 650 0 S97 R t.k7 CONFIDENCERAF 17:52. 4 070 TO .27.1.1 @,1,77 ' 'DD&nV,-IDDDDDL)DDoi6,VLi&LIZ pDo nn 3 SLOPE."= D @A FILE 'Fc "F"16 L%L DATA REPORT.: Basic 1'est minutes NUMBErl .-ntrul ----------- 9!3.86/ 77.90 9.3.5-9 7 sz. @@4 < '.4 16 4 4 15 7 7.5 CR CoGrjRRE,@'T 1n-N F,^,r O@ ElI L!U 441'6 NC ti AdJu-.tmeiit :.Nact r /IS 77e2 N 3 4C, I- 4z 4, 3I.S "is DDDDDDDOtir,)r)p,-@,)DbODDDDDDD DDJ ...........