Document mbv9zBNr0XvqVRdzm3OBw1KyZ

STUDY SUMMARY OF ENVIRONMENTAL AFFF AND COMPONENTS - 1991 EFFECTS OF LIGHT WATER BRAND TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may also be referredtoas PFOS, FC-95, or as a component of FC-203CE. (1Octanesulfoniaccid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was notnoted. The testsample isFC-203CE (2/21/89).Currentinformationindicatesitisa mixtureof 1.84% PFOS, 30% diethyleneglycolbutylether,60.23% water,2.56% Sultone foamer, 3.1% sodium octylsulfate2,.1% polyoxyethylenemonooctylphenyl ether,0.12% sodium laurylsulfatea,nd 0.05% tolyltdazoleT.he 3M product lotnumber was 519. The followingsummary applies to a mixture with incompletely characterized concentrations of impurities.Data may not accurately reflecttoxicityof the fluorochemical component of the testsample. RESULTS Degradability -Study BOD5 BOD10 BOD20 COD BOD20/COD ratio TotalOrganicCarbon (TOC) OECD Method 301 E Value (source*) 191 g/L (3M) 400 g/L(3M) 680 g/L(3M) 762 g/L(3m) 10.89(3M) 1220 g/L(3M) 88-90% biodegradabiliitny14 days (3M) Aquatic toxicity Study Algae (Selenastrumcapricomutum) 95-hrIC5o OECD ActivatedSludge Respiration InhibitioTnest 209 3-hrEC5o -Waterflea(Daphniamagna) 48-hrStatiLcC50 Microtox(Photobacteriumphosphoreum) 5-min.EC50 Value (source*) > 1000 mg/L (3M) None at 1000 mg/L >1000 mg/L (3M) 370 mg/L 15-min.EC5o 1260 mg/L 30-min.ECw 1230 mg/L Killifis(hFundulus heteroctitus) I 96-hrContinuous Flow LC5o 11400 mg/L (3M) Fathead Minnow (Pimephates promelas) 96-hrContinuous Flow LC5o >2000 mg/L (3M) 96-hrStaticLC50 >1000mg/L(3M) Any study marked with "3M" as the source includesstudiesdone at the request of 3M by labsother than the 3M Environmental Lab. DATA QUALITY Reliability:These studieshave been assigned Klimishrankings = 4. Values are from a summary listonly. OTHER Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul, Minnesota, 55133 Last changed: 6/28/00 I,r,,.&,R.e^p,ohrnStiucmamlary FaMS7,V-11-H To*.Patent& TechnicaClommunicationSservice-s 201-2C-12 Repo?tSummarYmustbe typewrittGeuni.delinaerseonreversseidei.freporItsprinteodn bothsidesofpapers,endtwocopiestoP & TCS. Document .DepartnmtNUff*W NumberL@Li_i022Z__ To Pike Iff Aud"s) Ho@el -Enxixmmental-Ea&infxx.ing PIOW Dwription 'Pr*o Numbw Rollution Control PAW Nuabw Loc oi .089 Dot*TyW 4-1-91 Emplw" Numbw(s) 295864 Omani-tw code EE&PC (Mwe additionaDl.Ns InAbstraW&rW F-PP Rpnparnh PAWTft --4E.nvironmental Keywords and Developmp-nt Effects of LIGHT WATER Brand AFFF and Components 'Poead C4vwW LIGI HT WATER, aqueous foam, FC-203,FC-206, ethyleneglycolb,utyldarbkol.sodium laurylsuffatDeu,ponol, sodium do docylsuffates,odium octylsuffatLe-,4640,tdethariolamineu,rea,TritonX-305',foamer,nimer, surfactardt,olyftdazole, erwironmentaleffectst,oxicitDya,phnia toxicitMyi,crotox,algaltoxicitfyi,shtoxicitdye,gradabilftbyi,ochemicaloxygen demand, BOD, chemicaloxygen demand, COD. Project Objective & Report Abstract 1.To add totheknowledge base ofertvironmentaelffectsofLIGHT WATER brand aqueous filmformirifgoams and the components used intheproducts.2.To identiftyoxiccomponents and to correlatecomponent and producttoxicfties. Thistechnicalreportincludesa summary ofenvironmentaleffectsdataon 3M's productsand thecomponents used in them. The datawas takenfrom thelfteratuarned fmm previousstudiesby the3M EnvironmentalLaboratory.The data foreach component and each producthave been evaluatedforcompleteness and accuracy and additionatlesting requi*rementshave be' en noted.Where sufficiednattaon thetoxiedyand degradabitdyof Individuaclomponents were availablet,hedata were evaluatedand recommendations regardingInclusionofthe coffiponerIdnfutureproductshave been made. An attemptwas made tocorrelattehetoxicitoyf FC-203 tothetoxicitoyf itsconyponentsby takinglrddponsideratiotnhe component concentratioinntheproduct.Whileproducttoxicitcyouldnot be corx*jsivelcyorrelatedtoth6 toxicitaynd concentratioonf individuaclomponents,majorcontftdorsto producttoxicitayre believedto be the filmert,hefoamer, butylCarb'dolTma,nd Duporiol7mME (sodiumlauryisuffatCeo)m.ponents likeltyoooritrbftleessto producttoxicitayre ethyleneglycolt,olyftdazolseo,dium octylsulatea,nd Di-water.Furthertoxicittyestingofcomponents and product formulationsshouldenablebettercorrelatiobnetween component and producttokuries. ReparlType Security C R & D Research and Development 0 PILot Plant 0 MANufacturing 0 Management summary :m Open ReDort and Summary Nolit,*Ftsterence lnfmmtlon Usimn irat1ws 0 TRP Trip or Field Report 0 FACtory Experiment 0 ENGineering 0 ROI Record of Invention 0 TECH. Service 0 Govt. Project 0 OTHER 13 Closed Report -open summary 3M Chemical 0 New Chemicals Reported Registry Lftfeormsm toantwintoChemicaRlegistry For P & TCS use Only FUm Go& Blo. N r ol Pages P & TCS EditorInitial& Contents 1.Purpose ........................*............................................................2................. 11.Background ..........................................................2................ IllG.eneralComments ...................................................4................ IV.Conclusionsand Recommendations ......................................6................ V. EnvironmentalEffectsUteratureSearch ...................................9................. 1.EthyleneGlycol(RM 3017) ...........................................1.0............... 2.ButylCarbfto(lRM 8887) ............................. 12 3.Duponol (RM 3021);DuponolME DrySurfactan(tSodiurTL!aurylSulfate)......1.4. 4.Tolyltdazol(eRM 27513;CorrosionInhibitToorlyltdazole.TechniGcraalde) .....1.6 5.Foamer (FM-4115) ..................................................1.8............... 6.Filmer(FM-3820or FC-95) ............................................1.8............... 7.Na-octylsulfa(tReM 32067 or RM 55040) ................................1.9.,.............. 8.DI-water(RD 199) ...................................................2.0................ 9.FC-203 .......................................t...............................2.1............................... 10.VW1 60390 (FC-203withoutTolyltdazolReM 27513) ..:.t.t..................2.3............. 11.FC-203CE ........................................................2.4............... 12.FC-206CE ........................................................2.5............... 13.FC-203CF ........................................................2.6............... 14.FC-206CF ...........................................I................2.6................ 15.L-4640(New Foamer) ..............................................2.6............... 16.Witcolla7t0e93 (RM 45666) ...........................................2.7............... 17.Tdethanolamine ...................................................2.7................ 18.Urea ............................................................2.9............... 19.Tdton X-305 ......................................................3.0............... EnvironmentaElffectosfUGHT WATERTM Aqueous FilmFormingFoam and-Components 1.Purpose The purposeofthistudyistoincreas3eM'sknowledgoeftheenvironmenteaflfecotfs LIGHT WATERTM aqueous filmformingfoam and the individuaclomponents used to make the product.And, by understandingthese effectst,o minimizethem throughformulationchanges. 11.Background This studywas undertakeninresponsetotestingdone by the German Army which was reportedin"InvestigatioonfsToxicEffectsand BiologicaDlegradabilftoyfFoam Extinguishing Agents inWastewaterm by Ekkehard lsingT.he report,hereafterreferredto as the "German Army Report",includeddata for16 firefightinmgaterialso,fwhich two were 3M's FC-203 and FC-206.The 16 firextinguishinaggentswere categorizedas: 5 multi-rangfeoam agents -6film-forminfgoam agents 3 proteinfoam agents 1 film-forminfgluo(ine-protfeoianm agent 1 specialfoam agent fordeep-frypan fires EnvironmentaleffectsconsideredintheGerman Army Report-were: 1.Acute toxicittyothe marine photoluminescentbacterium Photobacterium phosphoreum usingtheMicrotoxlsystem.The toxicitwyas expressed as F-C.la.nd ECw, however,theexposuretime isnotclearfrom the German Army Repd and may have been 10,20,or30 minutes.Two multi-rangefoam agents were considered "highlytoxic"T.hree multi-rangfeoam agentsand 2 filmformingagentswere found to be "toxic"F.our film-formiraflgl'3,proteint,he film-forminfgluorine-proteaignent,and the deep-fryagentwere consideredtohave "lowto)(idivF.P-203 and FC-206 were inthe "low toxicitcya"tegory. 2.Algae cellmultiplicatiionnhibititoenstusing Scenedesmus subspicatusas thetest organism withan exposure time of72 hours.Toxicitywas expressed as EC 10and ECN. Allfoam agentswiththeexceptionoftwo ofthe proteinfoam agents were "toxict"oalgae.The two proteinfoam agents had weak toxicittyoalgae.Both 3M productswere considered"toxietoalgae. 3.Acute toxicittyoDaphnia magna afterexposure for24 hours.The toxicitwyas expressed as LCIO and LC50,buttheeffectcriteriownas theorganisms'lossof ability to swim and willbe referretdo as EC 10and EC50 inthisreport.All5 multi-rangefoam agents,allfilm-forminaggentsexceptFC-206, and the deep-fryagent were "toxieto -2- DaphniaO.ne film-formaignegnt(FC-206)a,ltlhreeproteiangentsa,ndthe film-formfilnugodne-proatgeeinnthad"lowtoxicitoyD"aphniaFC-203:"toxic"; FC-206:"lowtoxidity'. 4.Acute toxicittyoZebrafish(Brachydanioredo)afterexposure for48 hours.The - -criterwiaas death and thetoxicitwyas expressed as LC 10and LC5,.Only two mult-rangefoam agentswere "toxietofish.Three multi-rangfeoam agentsand two film-forminaggentswere "borderlinteoxictwealdyto)Cic"rW.eakly toxidnwere 4 film-forminaggents,2 proteinfoam agents,and the deep-fryagent.One film-forming agentand thefilm-forminfgluodne-proteiangent were "non-toxie.FC-203 and FC-206 were inthe"weaklytoxic"category. 5..Chemical Oxygen Demand (COD) and 5-day BiochemicalOxygen Demand (BOD 5) ofa 3% solutionofthetestmaterialinwater.The ratioofBOD 5to COD was used as a ratingguidelinew,itha BOD &/COD ratiof 0.5 or lew consideredWinsufficient biochemicaldegradation"T.wo mulfi-rangeagents and -onefilm-forminaggent (FC-206)were consideredinsufficiendtelgyraded biochemicallyT.he ratioforFC-206 was 0.35.One multi-rangaegent and one filmformingagent (FC-203)were considered"probablyinsufficienbte"cause theirBOD WOOD ratioswere 0.55.The 9 remainingagentswere allsufficienWtiththeirBOD /COD ratiosrangingfmm 0.60 to 0.89. The data from theGerman Army Reportforthetwo 3M.products are summadzed inthetable below.The-data forthe Microtox,Zebrafish,COD, and BOD 5testsseem to be "reasonable* and "self-consistenTth"e.data are consideredreasonablebecause they agree fairlwyellwith data from previous3M studiesand areconsideredself-consistenbtecause the FC-206 data are roughlyhalfthatofFC-203. PmdLid FC-203 Licrowx EC,, ECSO $32 2,500 -Low Toxk*r Summary ofGerman Army Report Data Alps ECIO EC,. 16.9 160 To)de- DM*tva LC,, LCSO 31.6-* 430 *To)dcr Zebrafim LC" Len 1,053 1,634 -Wealdy Tox-e coo 41.2W BON BODWCOO Rado 21,500 0.52 - FO-2D6 2,04015,000 16T.o3)dd.160 'Lx)wToxwq Note:allconoontradonsinmgfl. 305 2,ODO 'Low Toxicitr 2,036 4,086 'Makly Toxie 24,600 - 8,700 - 0.35 'Insuffidenr -3- For thealgaltoxicitdyata,therearetwo importantpointstoconsider.Firstt,he German Army's algaedata show essentialleyquivalentoxicitfyorourtwo products.One would expect more toxicitfyorFC-203 than forFC-206, as shown inthe Microtox,Zebrafisha,nd Daphnia tests.However, highertoxicitbyy FC-203 than by FC-206 may notalways be true.For example,theremay be a component which isparticulartloyxictoalgae,perhaps disrupting thephotosynthetipcrocess.Second, our PED forFC-203 indicatenso effectotwo algal spediesatconcentrationusp to 1,000 mg/L.Whilethe two algalspecieson the PED are not thesame as those inthe German Army Report,they are allfreshwaterspeciesand such a largedifferencientoxicitwyouldn'tbe expected.One importantlimitatiwoinththe PED data is thatitdatesfrom 1974 and ithas been impossibletovedfythe exactformulationofthe sample used inthosetests. For the Daphnia data inthe German Army Report,the differencbeetween the*FC-206and FC-203 values.seemslargewhen consideringthewater contentofthetwo formulationsA.lso, thereisa considerabledifferencbeetween the PED data.andthe German Army data.Our PED has a 48-hrstaticDaphnia magna LC5. of1,600 mg/L,whilethe German. Army Report has a 24-hrstaticDaphnia magna EC50 is430 mg/L.The PED data isquiteold(1973)and the formulatioonfthetestsample cannot be verifiedT.here isa majordifferencbeetween the PED data and the German Army data inthatthe PED data istheconcentratiownhich causes death to50% ofthe Daphnia testpopulation(LC 50)and the German Army value isthe concentratiownhich causes 50% ofthetestpopulationtostopswimming (Effective Concentration= EC50).The German Army valueisinfairagreement withrecentresults which show a 48-hrstaticDaphnia magna EC50 of229 mg/L. One thingthatshouldbe keptinmind when consideringthetoxicitdyata isthatreproducibility and precisionoftoxicittyestscan vary considerablyfrom laboratortyolaboratoraynd over time.However,the differencebsetween the olddata(circa1974) and the more recentdata is more likeldyue tochanges informulationratherthan changes intestprotocolortestspecies. At any rate,our primaryinteressthouldbe thetoxicitoyf our currentproductsand Wia*.should be concerned withhavingcurrent,reliabldeata on our PEDS. IllG.eneral Comments The followingeneralstatementssummarize the factorsguidingthisstudy. 1.The primarypurpose of.theenvironmentaleffectsdata isforinternaulse inevaluating formulationasnd ingredientasnd not intendedforsubmissiontogovemmental regulatorayuthoritieTsh.erefore,any testingshould be done usinglow-cost, non-GLP methods.An exceptionmay be dataforthe new foamer (L-4640)which may need to be submittedforthe European PMN currentluynderway. 1 DraftReportfrom EnviroSystemsLaboratorydated March 10,1991.We have asked EnviroSystemsto repeatthe Daphnia testsforFC-203 and VW1 60390. The preliminary resultfsrom EnviroSystemson the restofthe samples were acceptable. -4- 2.The German Army performsitsown testingand no.authodtyotherthan the German Army ispresentluysingtheevaluationscheme used inthe reportcitedabove. Therefore,thereisno need tochoose a laboratoryt.estprotocolo,rtestspecies applicabloenlytotheGerman Army testscheme. Instead,testmethods and species shouldbe chosen so thattestresultcsan be used inas many countriesas possible. 3. Where possibled,ata on the environmentaleffectsofcomponents should be obta7ined from the literatuorrefrom raw materialsuppliersinorderto reduce testingcostsand time. 4.The Nagonal InstituotfeOccupationalSafetyand Health(NIOSH) terminologyforthe relativteoxicitoyfthecomponents and the formulationswillbe used inthe discussionoftox7iecffectsW.hiletheseterms were not used inthe German Army Report,theyare generallyaccepted inthe US. The toxicitaynd correspondingterms are: ConcentrationAcutely Toxicto Test Population NIOSH Terminology :51mg/L 1 -10 mg/L 10 - 100 mg/L 100 - 1,000 mg/L >1,000,mg/L 'Highlytoxicr "ModeratelyToxicr "SlightlTyoxidn "PracficallNyon-Tox7idu "InsignificTaonxtidtr 5.The LIGHT WATER AFFF formulationasnd components consideredare: 1.EthyleneGlycol(RM 3017) 2.ButylParbitol(ORM 8887) 3.DuponolO ME (RM 3021) 4.Tol*Yltdazol(eRM 27513) 5.Foamer (FM-4115). 6.Filmer(FM-3820 FC-95) 7.Na-octylsulfa(tReM 32067 or RM 55040) 8 Di-water(RD 199) 9.FC-203 10.VW1 60390 (FC-203 withoutTolyltdazolReM 11.FC-203CE (same as FC-203C) 12. FC-206CE 13. FC-203CF 14. FC-206CF 27513) -5- 15. L-4640 16.Witcolate7093 (RM 45666) 17.Tdothanolamine 18. Urea 19.TritonX-305 Items1 to10 are from a lisgtivento EricReiner by Wim Vanneste 3/12/90.Items II to13 were giventothe authorby Wim Vanneste 2/5/91.Items14 to16 were given tothe authorby Roger Altn(2/4/91). IV.Conclusions and Recommendations Inordertocorrelattehetoxic7iotfythe producttotheto)Cic7oiftiyndividuaclomponents,the component concentrationistakenintoconsiderationI.nthe tablebelow,thetoxicittyo Daphnia ofthe pure components are giveninthethirdccilumn.The fiftcholumn contains estimatedtoxic7ifibeased on productformulationT.his isthetoxicitwyhich would be expected when the component isdilutedinwatertothe same concentratiqnas inthe product.For example, one would expectthatsincethetoxicitoyfpure butylCarbitolOisabout 700 mg/L, thetoxic7iotfya solutionmade of 35% butylCarbitolOand 65% waterwould have a toxic7iotfy about 2,000. Some interestinogbservationscan be made from these numbers. Firste,ven though tolyftdazoliesquitetoxic,itispresentatlow concentrationinthe productand when dilutedin theproductitprobablydoes notcontributseubstantialltyoproducttoxicityH.owever, this analysisdoes notexoneratetolyhriazolaes a majorcontributotroproducttoxicityS.econd, the lowestnumbers inthefiftcholumn indicatceomponents whic@ willbe the most likely contributortsoproducttoxic7ibtuyt:ylCarbitolOD,uponol'PME (dodecylsulfatet)h,e foamer,and thefilme@rU.nfortunatelyt,hereisnota singlecomponent which standsoutwitha n'umberin thefiftcholumn which issubstantialllyowerthan allthe rest.This may mean thatth@'eri'sea synergistiecffectbetween components. .6- hmn Name 1. E( 9%39119@@ 2. ButylCarbbor (RM 8887) 3. Duponol*ME (RM 3021) 4. ToMMazob (RM 27513) S. Foamer (FM-4116) 6. Fdmor (FM,-AM F"5) 7. Na-octyleLdfate (RM 32DS7 orRM 55040) S. Di-water (RD 199) 9. FC-203 10. VW16039D (FC-203 w&.o4ATolyW=ole) SummaryofRecentDaphniaToxiciDtayM >1,000 FC-2D3 Formtda$on 20.00% mTwaidmdotny BCa::TcnnF"ot >5,000 706 (523-1,000) 35.00% 2,017 2.9(2.3-3.5) 0.10% 2PO 19(12-25) 0.05% 3SAW 68(50-81) 2.66% 206 49(43-56) 1.34% 3,07 517 (400-1,000) 3.00% 17,= imA@"@Utt"f? Probablynot Probably Probably Pmb ablyrat Probably Probably Probablynot >1,000 37.85% :..2.642 No 229 (150-444) x 165 tl28-248) x . The imp"ortanctonclusionfrom these Daphnia resultsisthata more complete set *oftoxicity data isneeded, especiallfyoralgae.The tablebelow summarizes theadditionadlata needed tocompletethe environmentaleffectsdatabase on our productsand theiringredients. Additionatlestsare indicatewdithan X. 2 Preliminarryesultfsrom EnviroSystems,March 10, 1991. -7- Summary ofAdditionaTlesting ft9m Nam 1. E(dRrn3eAlycol ( 301 2. ButylCarbiblO (RM 8887) 3. Duporol* ME (RM 3021) 4. ToWWazole (RM 27513) 5. Foamer (FM-4115) 6. Rknor (FL4,SM - FC-95) 7. Na-"Istdtate (RM 32D67 orRM 55040) 8. Di-water (RD 199) 9. FC-203 10. -VW160390 (FC-203 wiftutTolyftmle) li. FC-2D3CE (saineas FC-203C) .12. FC-2D6CE 13. FC-2D3CF 14. FC-2DSCF 15. L-4640 1& WiWWate 7093 (RM 45666) 17. Triedmwlartdne is. Urea 19. TdtDn X-305 TOTAL W=MX x x x x x x x x x x x x 12 Alpe DaOnW x x x x x x x x x x x x x x x x x x x x x 15 6 Reh x x x x x x x 7 Note:SOD testriwgliindude 5-,10-,and 20-daywsts. COD x x x x x x x x 8 BOD x x x x x x x x x x 10 -8- V.EnvironmentaElffectUsteraturSeearch Datafrompreviou3sM EnvironmentLaalboratoSrtyudieasndfromtheliterataurree summarized inthissectionT.oxicitdyata noted by "AQUIRE" were obtainedfrom a search of the USEPA AQUIRE database on Feb. 12,1991. Includedas partofthe referenceforthe datafrom AQUIRE arethe date ofpublicatioonfthe originajlournalarticlaend the review code establishedby a group ofEPA reviewersT.he reviewershave rankedto)(icidtaytafrom theliteratubrye thefollowinsgcale: 1.Meets allreviewcriteria. 2. Proceduresgenerallysatisfactory. 3. Insufficieinntsome area ofthetestmethodology ortestreport. 4.Abstractorforeignpaper iwitdhata inlimitedformat. The completecontentsofthe AQUIRE database foreach chemicalisnotgiveninthis'report because thedatabase includesmany obscure speciesof-littlienteres(topossum shrimp, Indiancatfishs,nakehead catfisho,ysters,clams,sponges and many others)and data which isconsideredby the EPA reviewerstobe ofquestionablevalue,. Additionadlegradabilitaynd toxicitdyata have been taken from the Handbook of EnvironmentalData on OrganicChemicals by KarelVerschueren (NostrandReinhold,New York,1983).These are referencedby page number inthe Handbook followedby the date of the originarleferenceT.here are many cases inwhich the same data appeared on AQUIRE and inV-erschuerenbutonlythe AQUIRE referenceisgiven because itincludesthe EPA qualityratingofthe originarleference. Raw materialsupplierwsere identififerdom the 3M PRISM database and supplierswere contactedand asked forany environmentaldata they have. Unfortunatelye,nvironmentaldata was notgenerallyavailablferom raw materialsuppliersA.nd, the supplierswere slow in sending thelimitedatado have. The source and reliabiloifttyheliteratudraeta was taken intoconsiderationinthe environmentalassessment ofthe components and the recommendations forfurthertesting. Repgrt Format: Informatiofnoreach component orformulationisorganizedas follows: Formulationorcomponent name and RM number ChemicalAbstractsService(CAS) Number Vendors Degmdabilitydatafrom theliteratuorrefrom previous3M assessments AquaticToxicitdyatafrom the literatuorrefrom previous3M assessments EnvironmentalAssessment Recommendations forAdditionaTlesting -9- Detailedinformatiofnorcomponents and formulationasre giveninthe same orderas inthe lisatbove. 1.EtoAqne Glycol (RM 3017) CAS # 107-21-1 Vendors:Chemtech, Ashland Chemical,Dow Chemical,Texaco Chemical,Olin,ShellOil, Union Carbide,TextileChemical,Ashland Chemical, Unocal Degmdabildy; TheoreticaOlxygen Demand (THOD) BODs BOD,O BOD15 BOD20 COD BOD./COD Ratio BOD2dCOD Ratio 1.29g/g (Verschuerenp 646) 0.44D.22g/g (avgof12 citations from Verschuerenp 646) 0.52g/g(Env.Lab.3Req. #1940) 0.67 g/g(Verschuerenp 646; 1953) 1.19g/g(Env.Lab.Req. #1940) 0.92 g/g (Verschuerenp 646; 1953) 1.14g/g (Env.Lab.Req. #1940) 1.00-+0.08g/g(avgof3 citations from Ve.rschuerenp 646) 1.02g/g(Env.Lab.Req. #1940) 1.240.04.g/g(avgof3 citations fr6m Vei@chueren p 646) 1.28g/g (Env.Lab Req. #1940j 0.4(Env.Lab Req. #1940) 0.8(Env.Lab Req.#1940) Toxicily Green Algae (Scenedesmus quaddcauda) 7-day StaticPopul.Growth Inhib5. 8-day staticPopul.Growth Inhib. 10,000 mg/L (AQU IRE 2;1980) 10,000mg/L (AQUIRE 2;1978) 3 3M EnvironmentalLab work done inDec. 1973. 4 The toxicitdyata are a partiallistinogf38 testson 16 spediesfrom the EPA AQUIRE database. 5 PopulationGrowth InhibitioCnh:ange incellnumber ofalgalspeciesincluding pre-exponentiallag rateeffects. -10- Protozo(aEntosiphsounicatUM) 72-hrStatiPcopulG.rowthInhib. 10,000mgAL(AQUIRE2;1980) Blue-greAelngae(Anacystaiesruginosa) 8-dayStatiPcopulG.rowthlnhib. 2,000mg/L (AQUIRE 2;1978) Bacteria(Pseudomonas putida) >10,000 mg/L (Verschuerenp 647) Algae (Microcystafesruginosa) 2,000 mg/L (Verschuerenp 647) Protozoa(Umnema parduczi) >10,000 mgtl (Verschuerenp 647) Water flea(Daphniamagna) 48-hr StaticECBO 24-hr StaticLC50 24-hr StaticLC50 48-hrStaticLC50 >1.000 mg/L (EnvimSystems 1991)6 10,000 mg/L (AQU IRE 2;1977) 10,000 mgAL (AQU IRE 3;'1983) 10,000mg/L (AQUIRE 3; 1983) Brineshrimp(Attemiasalina) 24-hrStatiLcCso 24-hrStaticLCw 20,000 mg/L (AQU IRE 2;.1974) 20,000 mg/L (AQUIRE 3; 1983) FatheadMinnow (Pimephalespromelas) 24-hrStatiLcCSO 48-hrStaticLC50 96-hrStaticLC50 96-hr StaticLCso 10,060 mg/L (AQU IRE 3;1983) 10,000 mg/L (AQU IRE 3; 1983) 10,000 mg/L (AQU IRE 3; 1983) 49,000 mg/L, 53,000 mg/L, & 57,000 mg/L (AQUIRE 2; 1983) BluegilSlunfish(Lepomis macrochirus) 96-hrStatiLcCO 10,000r@g/L(AQU IRE 2;1968) FWinbow Trout(Salmogairdnen) 96-hrStaticLCso 41,000 mg/L (AQU IRE 2; 1960) Common Shrimp (Crangon crangon) 48-hrRenewal LC50 96-hrRenewal LC5. 100,000 mg/L (AQUIRE 2;1974) 50,000 mg/L (AQUIRE 2;1974) Goldfish(Carassiusauratus) 96-hrStaticLC50 5,000mg/L (AQUIRE 2; 1979) Guppy (Poodliareticulata) 7-dayRenewal LCso 49,300 mg/L (AQUIRE 3;1981) 6 Acute Daphnid toxidittyestingdone by EnviroSystems for3M. Test resultsdated March 10, 1991. Environmental-Assessment: Ethyleneglycolhas insignificatnotxicitAynd a high BOD/COD ratioE.ven though itis presentinhighconcentrafi.ionthe product,itprobablydoes notcontributseubstantialltyo producttoxicity. Recommendations forAdditionaTlesting: None. 2. Butyl CarbitolO(RM 8887) CAS # 112-34-5 (DiethyleneGlycolMonobutylEther) gi6onyms and vendors:ButylDio)Cdo(lCainChemical),Poly-SolvDB (OlinCorporation), GlycolEtherDB (Oxychem), ButylCarbftolO(UnionCarbideCorporation- LindeDiv.), ButylDioxito(lShellCriClompany), Dowanol DB (Dow Chemicaj),Ektasolve-DB(Eastman Chemical Products,Inc.) Degradabift THOD BOD5 BOD,O BOD,5 BOD20 COD BOD./COD Ratio BOD2dCOD Ratio Toxicily: Bacteria(Pseudomonas putida) 2.17 g/g(calculated) 0.25(Verschuerenp 524) 0.69g/g(Env.Lab.7 Req.#1940) 1.45g/g (Env.Lab.Req. #1940) 1.61g/g (Env.Lab.Req. #1940) 1.56g/g (Env.Lab.Req. #1940) 2.08 (Verschuerenp 524) 1.83g/g(Env.Lab.Req. #1946)"@ 0.12(Verschuerenp 524) 0.37 (Env.Lab.Req. #1940) 0.85 (Env.Lab.Req. #1940) 255 mg/L (Verschuerenp 524) 7 3M EnvironmentalLab work done inDec. 1973. 8 The toxicitdyata are a partiallistinogf15 testson 10 speciesfrom the EPA AQUIRE database. -12- GreenAlgae(Scenedesmus quadricauda) 7-dayStatiPcopulG.rowthlnhib. 8-dayStaticPopul.Growth lnhib. 1,000 mg/L (AQUIRE 2;1980) 1,000mg/L (AQUIRE 2; 1978) Blue-greenAlgae (Anacystiaseniginosa) 8-dayStaticPopul.Growth lnhib. 53 mg/L (AQUIRE 2;1978) Protozoa(Entosiphonsulcatum) 72-hrStaticPopul.Growth lnhib. 73 mg/L (AQUIRE 2;1980) Protozoa(Uronema parduczi) 420 mg/L (Verschuerenp 524) Protozoa(Chilomonasparamedum) 48-hrPopul.Growth Inhib. 2,7.74mg/L (AQUIRE 4; 1980) Water Flea(Daphniamagna) 48-hrStaticEC50 24-hrStaticLCso 706 mg/L (EnviroSystems1991) 2,850 mgtl (AQUIRE 2; 1977) BluegilSlunfish(Lepomismacrochirus) 96-hrStaticLC50 1,300mg/L-(AQUIRE 2; 1977) Goldfish(Carassiusauratus) . 24-hrStaticLC50 2,700mg/L (AQUIRE 2;1979) Guppy (Poeciliraeticulata) - 7-day Renewal LCso 1,150mg/L (AQUIRE 3; 1981) Silveirde(Leuciscusidus) StaticLC50 1,805mg/L and 2,304mg/L (AQUIRE 3; 1978) InlandSilversid(eMenidtaberyllina) 96-hrStaticLC50 2,000 mg/L'(AQUIRE 2; 1977).. EnvironmentalAssessment: BOD data shows f'aidhyighdeg!a@dabiliTthye. literaturdeata show thismaterialto have insignificatnotxicittyo fishand Daphnia The two freshwatergreen algae values from AQUIRE areconsistenta,nd indicattehat..blfCtaorbitolOispmcticallynon-to)(itcogreen algae.There isconsiderablymore toxicitsyhown to blue-greenalgae,however. We should have a sample ofbutylCarbito0ltestedforalgaltoxicittyobe sure ofitstoxiceffects. Recommendations forAdditionaTlesting: Algae and Microtox. 13- 3.DuponolOME DrySurfactan(tRM 3021;Sodium LaurylSulfate) CAS # 151-21-(3SodiumDodecylsulfCaHt3e(CH2)11OS03Na) Vendor: DupontO Degradabift THOD BOD Toxidity: Green Algae (Chlorelisap) . StaticPopul.Growth Inhib. Green Algae (Chlorelivaulgails) 72-hrStaticGrowth Inhibiti12on Green Algae (Scenedesmus quadficauda) 7-dayStatiPcopul.Growth lnhib. 8-day Renewal Growth Inhibition FlagellatEeuglenoid(Entosiphonsuicatum) 72-hrStaticPopul.Growth lnhib. Blue-greenAlgae(Anacystiaseruginosa) 8-dayRenewal Popul.Growthlnhib. Bacteda (fteudomonas putida) CellMultiplicatIinohnibition Protozoa(Uronema parduczi)CellMultiplicatiIonnhibition 2.03 g/g (calculated) SvvisheOO reportsthat"linearprimary alkylsulfateshave long been .recognizedas extremelyrapidin primarybiodegradafiofoif,ten disappearinginlessthan a day" and dttesapproximately100 references which show degradationratesof80 to 100% Withina few hours or days. 50 mg/L (AQU IRE 4;1980) 0.05mg/L (AQUIRE 4) 0.02m@it (AQUIRE 2;1980) 0.02 mg/L (AQUIRE 2; 1978) 40 mg/L (AQUIRE 2;) 7 mg/L (AQUIRE 2;1978) 290 mg/L (Verschuerenp 793;1980) 0.75 mg/L (Verschuerenp 793; 1980) 9 Phone: 302-774-1000.2/15/91:willsend information. 10 Swisher,R. D. SurfactantBiodegradation2.nd ed, Marcel Dekker:New York, 1987,p 466, 807-811. 11 The toxicitdyata are a partiallistinogfthe 409 testson 49 speciesfrom inthe EPA AQUIRE database. 12 Growth InhibitioMne:asurable change inlengthand/orweight. -14- Waterflea(Daphnimaagna) 48-hrStatiEcC50 24-hr StaticLC50 24-hrStaticLC50 48-hr StaticLC50 48-hr StaticLC50 .48-hrFlow-througLh050 2.9 mg/L (EnviroSystems1991) 33.0mg/L (AOUIRE 2; 1977). 7.2,10.0.& 12.0(AOU IRE 3; 1983) 7.02.1mg/L (avg of8;AQUIRE 2; 1982) 5.00.5 mg/L (avg of 3;AQUIRE 3; 1983) 1.8mg/L (AQUIRE 2; 1981) Fathead Minnow (Pimephalespromelas) 24-hrStatiLcC50 48-hrStaticLCw 96-hrStaticLC50 .7.4& 8.5 mg/L (AQUIRE 3;1983) 6.6& 7.3mgtl (AQUIRE 3; 1983) 6.6& 6.9 mg/L (AQUIRE 3; 1983) Bluegil(lLepomismacrochinis) 96-hrFlow-throughLC5. 4.5 mg/L (AQUIRE 2;1981) Mummichog (Fundulusheteroclitus) 96-hrStatiLcC50 2.1mg/L (AQUIRE 2) Sheepshead Minnow (Cyptinodonvadegi3tus) 24-hrStaticLC50 10.0mg/L (AQUIRE 2) 48-hr StaticLC50 9.0mg/L (AQUIRE 2) 96-hr StaticLC50 9.0 mg/L (AQUIRE 2) High-eyosMedaka (Oryziaslatfpes) 6-hrRenewal LC50 24-hrRenewal LC50 48-hrRenewal LC50 67 mg/L (AQU IRE 2) 46 mg/L (AQUIRE 2) 46 mg/L (AQU IRE 2) Bdne Shrimp(Artemiasalina) 24-hrStatiLcC5. 3.6mg/L (AQUIRE 2;1974) AtlanfiScilversid(eMenidiamenidia) 96-hrStatiLcC50 2.8mg/L (AQUIRE 2;1982) t(illif(iFsuhndulussimilis) 24-hrStatiLcC50 48-hr StaticLC50 96-hrStaticLC, 4.7mg/L (AQUIRE 2) 4.7mg/L (AQUIRE 2) 4.5 mg/L (AQUIRE 2) Ra7in0owTrout(Salmogairdnen) 48-hrFlow-througLhC50 96-hrFlow-throughLC50 10-day Flow-throughLC50 5.95 mg/L (Verschuerenp 793; 1977) 4.62 mg/L (Verschuerenp 793; 1977) 2.85 mg/L (Verschuerenp 793; 1977) -15- Zebrals(hBrachydanrieorio) 48-hrFlow-throuLgCh50 96-hr Flow-throughLC50 lo-day Flow-throughLC50 8.81 mg/L (Verschuerenp 793; 1977) 7.97 mg/L (Verschuerenp 793; 1977) 7.97 mg/L (Verschuerenp 793; 1977) Flagfis(hGordanellaflotidae) 48-hrFlow-througLhC50 96-hr Flow-throughLC50 lo-dayFlow-throughLC. 10.0 mg/L (Verschuerenp 793;1977) 8.10 mg/L (Verschuerenp 793;1977) 6.90 mg/L (Verschuerenp 793; 1977) EnvironmentalAssessment: Literaturdeata indicatefsairllyow toxicittyobacteriaand althoughno actualBOD testdata are availablet,hismaterialshouldbe readilydegraded by microorganisms.A BOD value shouldbe obtainedby ourown BOD tests. This materialismoderatelytoxicto fish,DaphniA and algae.Our most recentDaphnia test data agreeswellwithliteratudraeta which gives us confidenceinthecontractlaboratory's work on thismaterialB.ecause thereissufficiefnitshtoxicitdyata from the literaturneo,fish testingiswarranted. Because ofthe algaltoxicitsyhown inthe literatudraeta,algaetestingshould be done. Even thoughthismaterialistoxictoa varietyofaquaticorganisms,itreadildyegrades and adverseeffectswillbe minimalifitispassed througha wastewatertreatmentsystem orifa significantumber ofmicroorganismsare presentina naturalwatertodegrade itrapidly. We shoulddo some studiesinwhich our productispasses througha model wastewater treatmentsystem and the measure thetoxiceffectsofthe model'seffluentA.nother study would be a simulationofa naturalbody ofwater using''anaquarium stockedwithfish.This would giveus an understandingofthedegradationina riverp,ond,or lake. Recommendations forAdditionaTlesting: BOD, COD, and Microtox. 4.Tolyltriazol(eRM 27513; Corrosion InhibitorTolyltriazoleTechnical Grade) CAS # 29385-43-1 C7H7N3 N N H Synonyms and vendors: Methyl-1H-Benzotdazole -16- Methylbenzotdazole Cobmtec TT-100 (PMC, Inc.13) PreventolC17-100 (Mobay Corp.14) Dggmdabolfty; BOD Toxidjy: Water Flea(Daphniamagna) 48-hrStatiEcC50 BluegilSlunfish 96-hr LCso Rainbow Trout 96-hrLCso Fathead Minnow 96-hrLC50 Additionadlatafora similacrompound:. IH-Benzotdazole(C6H6N3) CAS Number: 95-14-7 Synonyms: 1,2,3-Benzotdazole Aziminobenzene Benzene Az7imide Benzotdazole Bluegilslunfish(Lepomis macrochinis) Static24-hrStress Rainbow Trout(Salmogairdnen) Stati2c4-hrStress Sea Lamprey (Petromyzonmadnus)'Static24-hrStress Chinook Salmon (Oncorhynchustshawytscha) Static24-hrMortality <60% THOD (Mobay Corp.) 19 mg/L (EnviroSystems1991) 31.0mg/L (Mobay Corp.and PMC) 2@l.4 mg/L (M-obayCorp.and PMG) 25.5 mg/L (Mobay Corp.and PMC) H 5 mg/L (AQUIRE 3;1957) 5 MGALJAQUIRE 3; 1957) 5 mgAL (AQUIRE 3;,1957) 10 mg/L (AQU IRE 3-1969) 13 PMC SpecialitiGersoup, Inc.TechnicalBulleti3n200. Contact:BillMautieWicz (513-242-3300). 14 Mobay Corp.datagivenby Matthew Bartman 412-777-2000. -17- Coho Salmon(Oncorhynchkuissutch) Stati2c4-hrMortality 10 mgA-(AQUIRE3;1969) NortherSnquawfis(hPtychochoiolruesgonansis) Stati2c4-hMrortality 10 mg/L (AQTJIRE 3; 1969) EnvironmentalAssessment: While the limitedatashows thatthismaterialismoderatelyto)Cici,tispresentatsuch low concentration(0.050/oi)ntheproduct,itmay have littelfefectinthe overalltoxicitoyfthe product.Because ofthetoxicitoyfthismaterialw,e need a complete setofdata.There is no reason torepeatthefishdata,however. Thismaterialislisteodn theTSCA 8(d)lisatnd any testingdone must be reportedtothe US EPA. Recommendations forAdditionaTlesting* BOD, COD, algae,and Microtox. 5.Foamer (FM-4115) Toxicity: Water Flea (Daphniamagna) 48-hrStaticEC50 68 mg/L (EnviroSystems1991) EnvironmentalAssessment: The foamer shows slightoxicittyo Daphnia,butthereisno data forthe remaining environmentaleffectsW.e should have a completesetofeffectsdataforthismaterial. Care skouldbe takenthatthesample used inthe environmentaleffectstestingis.*-agood representativseample which has been produced by the manufacturingmethod v@@!6hwill be used inthefuture. Recommendations forAddftionalTesting: BOD, COD, fish,algae,and Microtox. 6.Filmor (FM-3820 or FC-95) Degradabift BOD5, BOD,O BOD20 .COD BOD/COD Toxidly: nil(PED) nil(PED) nil(PED) nil(PED) 0 WaterFlea(Daphnimaagna) 48-hrStatiEcC50 48-hrStaticECso 4@ mg/L (EnviroSystems1991) 50 mg/L (PED) ----ActivateSdludge InhibitorCyonc. >4,000mg/L (PED) FatheadMinnow (Pimephalespmmelas) 96-hrLC50 38 mg/L (PED) BluegilSlunfish(Lepomismacrochirus) 96-hrLC50 68 mg/L (PED) Rainbow Trout(Salmogairdnen) 96-hrLC50 11 mg/L (PED) EnvironmentalAssessment* The filmerwillnotcontributteothe BOD/COD ratios,o toxicitiysthe Issuewfththis component. Thismaterialdoes show slightoxicittyofishand Daphnia.There Isgood agreement between previousand presentDaphnia toxicitdyata which givesus confidence inthe most recentdata from EnviroSystems. We need to have a completesetofeffectsdata fora sample representativoefa recent manufacturinglotwhich has been produced by the manufacturingmethod which willbe used inthefuture. Recommendations forAdditionaTlesting: Algae and Microtox. 7.Na-octylsulfate(RM 32067 or RM 55040 15) 142-31-4 Vendors*Alcolaclncorpomted'6(Sodium OctylSulfateSolution)S;tepan Company 17 (POLYSTEP B-29). Degradabift THOD 1.88g/g(calculate-d) 15 Wim Vanneste'sPROFS notedated 03/12/1990 givesthisRM#. 16 Alcolac:301-355-2600.No data available. 17 Called708-446-7500 ext.2283:they have no data. BOD Sm(ishe0areportsthat"Iniearprimaryalkylsulfateshave longbeen recognizedas extremel'yrapidinprimaryViodegradafiono,ftendisappearing inlessthana day"and citesapproximately100 referenceswhich show degradationratesof80 to 100% withina few hoursordays. Toxielly9e AustralianBarnacle (Eiminiusmodestus) 30-min StaticImmobilizationEC50 3,970mg/L (AQUIRE 2;1976) Water Flea(Daphniamagna) 48-hrStaticECio 517 mg/L (EnViroSystems1991) EnvironmentalAssessment* Althoughno BOD orCOD data areava@ilablteh,ismaterialwillmost likelhyave a high BOD/COD ratioB.OD and COD datashouldbe obtainedso thatwe have numericalvalues which have been obtainedintestscomparabletothoseused fortheremaining components. There issurprisingliyttlieteratudraetaavailabloen theaquatictoxicitoyfthissurfactant, however fortheDaphnia dataitshows considerabllyesstoxicittyhanthe C12surfactant. Thissurfactanitspresentat3% so itmay be d majorfactortotoxicitoyfthe product.Fish, algae,and Microtoxtoxicitdyataareessentiatlocompletetheassessment ofthis ingredient. Recommendations forAdditionaTlesting: BOD, COD, fisha,lgae,and Microtox. 8.Di-water(RD 199) Toxicilys Water Flea(Daphniamagna) 48-hrStaticEC50 >1,000 mg/L (EnviroSystems1991) EnvironmentalAssessment: Whileitmay seem foolisthotestthe.deionizedwaterforto)Cidttyo,xiceffectshave been tracedtocontaminateddeionizedwatersuppliesinthepast.The Daphnia resultincricates thatthesupplywas notcontaminatedand no furthetrestingofthedeionizedwatersample iswarranted. Recommendations forAdditionaTlesting: None. 18 Swisher,R. D. SurfactanBtiodegradation2,nd ed,Marcel DekkereNew York,1987,p 466, 807-811. 19 There was 1 testgivenintheEPA AQUIRE database. -20- 9.FC-203 Dogradabolfty; BOD5 BOD20 COD BODWCOD Toxifte Microtox EC,o EC50 ActivatedSludge Inhibition Algae (Chlorelipayrenoidosa . andPhormicrinuumndatum) Algae(Scenedesmsuusbspicatus) 72-hSrtatiPcopulG.rowthInhiEbC.lo 72-hrStaticPopul.Growth Inhib.EC50 Water Flea(Daphniamagna) 24-hrStaticECIO 24-hr StaticEC50 48-hr StaticEC50 48-hrStaticE050 560 g/L(PED) 717 g/L (German Army Report2O) 1,060g/L(PED) 1,070g/L(PED) 1,373g/L(German Army Report) 0.52 (PED and German Army Report) 632 mg/L (German Army Report) 2,500.mg/L (German Army Report) none at 1,000mg/L (PED) no effectat:91,000mg/L (PED 21) 16.9 mg/L (German Army Report) 160.'Mg/L(German Army Report) 31.6'mg/L(German ArffW.Report) 430 mg/L (German Army'Rbport) 229 mg/L (EnviroSystems1991)12 1,600 mg/L (PED23) 20-n3% applicatiosnolutionsw"ere used toobtain*theBOD5an d COD data inthe German Army Report.The German Army valueswere 41,200 mg/L and 21,500 mg/L forthe COD and BODR,,respectivelTyh.e German Army valueswere multipliebdy 33.3 to getthevalues inthisreport. 21 Testingdone by industriaBlio-TestLaboratory,Jan. 1974. 22 EnviroSystems isrepeatingthistestbecause therewas notenough variatioinnthe FC-203 concentrabonsthey used todevelop a good standardcurve. 23 Testingdone by Bionomics Laboratory,Dec. 1973. -21- EasternoysterEmbryo-larvae (Cmsostrea virginica) 48-hrStatiEcC50 47 mg/L (PED24) ----GrassShrimp (Palaamonatospugio) 96-hrStatiLcC50 510 mg/L (PED25) Fatheadminnow (Pimephalespromelas) 96-hrStaticLC50 750 mg/L (PED) Zebr.afi(sBhrachydaniorerio) 48-hrStatiLcCO 48-hr StaticLC50 1,053mg/L (German Army Report) 1,634mg/L (German Army Report) Said (Gammarus fasciatus) 48-hrStaticLC50 1,100mg/L (PED26) Rainbow trout(Oncorhyncusmykiss27) 96-hrStaticLCm 1,300mg/L (PED) Rainbow trout(Oncorhyncusmykiss) 96-hrContinuousFlow LCso 400 rhg/L(PED28) Common mum michog (Fundulus heteroditus) . 96-hrStat* icLC50 2,50 mg/L (PED29) Grass Shrimp (Palaemonetespuglo) 96-hr LC50 510 mg/L (PED) EnvironmentalAssessment: 1.The fishtoxic7idtaytafrom the German Army Report agree'swellwithdata from previous 3M studiess,o no fishtestingisneeded. 2.The Microtoxtestsfrom theGerman Army Report seem reasonable,so thist@4-tho'uld notbe repeated. 3.The 24-hrEC50 Daphnia toxic7iftryom the most recentEnviroSystems work and the 48-hr EC50 from theGerman Army Reportare infairlgyood agreement. However, we weren't satisfiewdiththe concentrationussed inthe EnviroSystems workand they are repeafing 24 Testingdone by Bionomics Laboratory,July1980. 25 Testingdone by Bionomics Laboratory,Aug. 1979. 26 Testingdone by Bionomics Laboratory,Dec. 1973. 27 FormerlySalmo gairdned. 28 No mortalitoyrabnormal effectsatthisconcentration. 29 Testingdone by BionomicsLaboratory,Aug. 1979. -22- thiswork.The largedifferencebetween.German Army valueand the 1973 PED value may be due toformulationchanges orchanges inthe toxicittyestprotocolbutthiscannot be verified. 4.As vvdhthe foamer and flimerw,e need tobe carefulthatthe sample submittedfor testingisofknown compositionand from a known manufacturinglot. 5.Sincetoxicittyoalgaewas ofconcern inthe German Army Report,the algae data is probablythe most important. Recommendations forAdditionaTlesting: BOD, COD, and algae. 10.VW1 60390 (FC-203 without TolyltriazolReM 27513) Toxidity: Water Flea (Daphnia magna) 48-hrStaticEC50 165 mg/L (EnviroSystems1991)30 EnvironmentalAssessment: 1.Sincetheonlydifferencebetween thismaterialand FC-203 istolykdazolet,estingthis materialajongwithFC-203 seems likea good way to_determinetheeffectoftolyltdazole. Forthe recentDaphnia data,thereislittdliefferencbeetween FC,203 and VWI 60390 and the effectoftolyltdazolceannot be seen. Infact,the toxicitpyfFC,203 was slightllyess than the toxicitoyfVW1 60390 inthe recertEtnviroSystems work. Since EnviroSystems is .repeatintghe Daphnia forthese two samples, we willhave towaituntilEnviroSystems has completed thesetestsbeforemc,Ong an evaluation. 2.A Micmtox and fishtoxicittyestshouldbe done on thismaterialand the results compared tothe FC-203 resultstosee ifthereisa measurable effectcaused by tolyltdazolienthe product. Recommendations forAdditionaTlesd= BOD, algae,fish,and MicrotoX.- 30 Envir6Systemsisrepeatingthistestbecause therewas notenough variatioinnthe concentrationtshey used todevelop a good standardcurve. -23- 1.FC-203CE Degmdabift BOD,s 91 g/L(PED) BOD,O 400 g/L (PED) BOD2o 680 g/L(PED) COD 762 g/L(PED) BOD /COD ratio 0.89(PED) TotalOrganicCarbon (TOC) 220 g/L(PED) OECD Method 301 E 88-90% biodegradability in14 days (PED) Toxidilye Algae (Selenastrum capticomutum) 95-hr IC50 >1,000 mg/L (PED) OECD ActivatedSludge Respiration InhibitioTnest 209 none at1,000 mg/L Water Flea (Daphnia magna) 48-hr StaticLCso >1,000 mg/L (PED) Microtox (Photobactelium phosphoreum) Expos'ure: EC50 5 min. 370 mg/L 15 min. 30 min -260rnb.& N, 230 mg/L Villifi(sFhundulushateroclitus) 96-hrContinuousFlow LC50 1,400mg/L (PED) FatheadMinnow (Pimephateipromelas) 96-hrContinuousFlow LC50 96-hr StaticLC50 >2,000mg/L (PED) >1,000 mg/L (PED) EnvironmentalAssessment: The environmentaleffectsdata forFC-203CE isfairlcyomplete. The PED data shows lowertoxicittyoDaphnia than isshown by FC-203 intheGerman Army Reportand from the recenttestsby EnviroSystems.Daphnia testingshould be done again forassurance inour PED. Recommendations forAdditionaTlesting: Algae and Daphnia. -24- 12.FC-206CE Degmdabilhz BODs BODIO' BOD20 46 9/L (PE -D) 260 g/L(PED) 470 g/L(PED) COD. 402 g/L (PED) BODMICOD ratio 1.2(PED) TotalOrganicCarbon (TOC) 130 g/L (PED) OECD Method 301E T6xicilx: 89-92% b7iodegradabilfty in14 days (PED) AJgae (Selenastrumcapticomutum) 95-hr IC50 >1,000 mo/1-(PED) OECD ActivatedSludge Respiration InhibitiToenst 209 none at1,000 mg/L Water Flea (Paphnia magna) 48-hr StaticLC50 >1,000 mg/L (PED) Microtox(Photobactetiumphosphoreum) Exposure: 5 min. 15 min. 30 min EC50 650 mg/L -4500j@@ 4200 mgAL Killifi(sFhundulus hateroclitus) 96-hrContinuous Flow LC. >2,000 mgAL (PED) Fathead Minnow (Pimaphalotpromelas) 96-hrContinuousFlow LCso 96-hrStaticLC., >2,000mg/L (?ED) >1,000 mgAL (PED) EnvironmentalAssessment: The environmentaleffectsdata forFC-203CE isfaidycomplete. Again,the PED datashows low toxicittyo Daphnia which shouldbe vedfied. Recommendations forAdditionaTlesting: Algae and Daphnia. -25- 13.FC-203CF EnvironmentAaslsessment: No environmentaleffectsdata forthisproductareavailableand a complete setof envimnmental effectsdata shouldbe obtained. Recommendations forAdditionaTlesting: BOD, COD, algae,Daphn!A fish,and Microtox. 14.FC-206CF E ironr@entaAlssessmento No environmentaleffectsdataforthisproductare availableand a complete setof environmentaleffectsdata should be obta7ined. Recommendations forAdditionaTlestinge BOD, COD, algae,DaphniA fisha,nd Microtox. 15.L-4640 (New Foamer) Testingiscurrentlyunderway fora European PMN by NOTOX Laboratories: 1.Daphnia toxicitsytudy(C2 EEC testmethod). 2.Fishtoxicit(yCl method) usingZebra fish(Brachydanioredo). 3.-ModifiedSturm test(C5)forreadybiodegradability. Allteitingforthe European PMN isbeingdone by GL@P. The followinegnvironmentaleffectdsata arefrom several3M technicalreportswhich are compiledinthe report"EnvironmentalData and InformationinSupportofthe PMN for Certa7in-ChemicaSlubstances Designatedas L-4640",,June':2108,80. Degradabift BODs BOD,O BOD20 COD TheoreticalOxygen Demand Toxidily: Acut6 Effecton MicrobialRespiration Water Flea(Daphniamagna) 48-hrStaticLC50 Fathead Minnow (Pimephalespromelas) 96-hrStaticLC50 <0.038 g/g <0.038 g/g <0.038 g/g 0.41 g/g 0.75g/g 8% reductioninrateofoxygen depletionat1,000 mg/L 100 mg/L and 111 mg/L 158 mg/L and 166 mg/L -26- EnvironmentalAssessment: Because ofthe importanceofthismaterialtofutureproducts,a fulsletofenvironmental effectsdata isneeded.The environmentaleffectsdata not includedinthe PMN work orin the-previoustudiesshouldbe obtained:algae and Microtox. Recommendations for-AddftionTaelsting: Algae and Micmtox. IS.Wkcolate 7093 (RM 45666) CAS # 73665-23-2 Wdco Corporation(713-433-7281)has no ecotoxinformationavailableW.deblate 7093 is 550/owater,450/oC6-Cl 0 ethoxylatedsulfateT.hey recommended SvAsher for biodegradationinformation. No informationon EPA AQUIRE database nor inVerschuere'n, EnvironmentalAssessmento No effectsdata isavailableT.estingdepends on the likelihootdhatthismaterialwillbe used infutureproducts.@ Rgcommendations forAdditionaTlesting: CODI- BOD, algae,f.ish,Daphnia,and Microtox. 17.Triethanolamine CAS 102-71-6 Dogradabilfty" THOD BODs BODIO, BOD15 BOD20 2.04 g/g nil 0.02 g/g withstandardseed 0.17 g/g adapted sewage 0.03 g/g 0.02 g/g nilwithstd.dilutedsewage 0.05 g/g 0.13 g/g 31 Alldegradabilitdyata are from Verschueren p 1150-1. -27- COD BOD51COD BODOICOD Raw Bactod(aPseudomopnuadsda) PopuGlm.wthlnhib. Algae (Microcysdsaeruginosa) Popul.Gmwth Inhib. Green Algae (Scenedesmus quaddcalda) 7-dayStatiPcopul.Growth lnhib. 8-day StaticPopul.Growth Inhib. 8-day StaticPopul.Growth lnhib. Blue-greenAlgae (Anacystiaseniginosa) 8-dayStaticPopul.Growth lnhib. Protozoa(Entosiphosnuicatum) 72-hrStatiPcopulatioGnrowth Protozoa(Chilomonasparamecium) . 48-hrRenewal Popul.Gmwth lnhib. Protozoa (Uronema parduczi) Bacteria (Pseudomonas) LCO Algae (Scenedesmus) LCO Algae (Colpoda) LCO Water Flea(Daphnia) LCO 24-hrStaticLC50 BrineShrimp(Artemiasalitia) 24-hrStatiLcC5. 1.509/9 nilto 0.11 0.09 >10,000 mg/L (Verschuerenp 1151) 47 mg/L (Verschuerenp 1151) 1.8mg/1-(AOUIRE 2;1980) 1,.8mg/L (AQUIRE 2;1078) 715 mg/L (AQUIRE 2;1978)33 19 mg/[-a.nd 47 mg/L (AQUIRE 2; 1978) 56 mg/L (AQUIRE 2;1980) 1,768mg/L (AQUIRE 4; 1980) >10,000 mg/L (Verschueren p 1151) 10,000 -mg/L (Verschuerenp 1151) 160mg/-C'(Verschuerenp 1,1@51) 160 mg/L (Verschuerenp 1151) 2,500 mg/L (Verschuerenp 1151) 1,390 mgtl (AQUIRE 2;1977) 5,600 mg/L (AQUIRE 2;1974) 32 The toxicitdyata are a partiallistinogfthe 16 testson 10 speciesgiveninthe AQUIRE database. 33 Thisvalueisfrom a differenrteferencethan the previousone. -28- Goldfi(sCharassiauusratus) 24-hrStatiLcCm 24-hrLCso(85% TEA) 5,000 mg/L (AQUIRE 2;1979) 75,000 mg/L atpH 7 3,500 mg/L atpH 10.3 (Verschuerenp 1151) EnvironmentalAssessment* This materialshows insignificatnotxicittyo Daphnia and fish. However, ithas a low BOD/COD ratioand iwilclontributteo a low ratiointhe product.In additioni,tshows considerabletoxicittyo algae.Forthese reasons,Itwould be bestnotto use thismatedalinproductsunlessno alternativcean be found oritsconcentrationinthe productwillbe extremelylow. The literatudraeta isessentiallcyompleteforthismaterialA.ny testingon thismaterialmust be reportedtoUS EPA under sectionTSCA B(d). Recommendations for-AddftionTaelsting: None. 18. Urea CAS 57-13-6 DegradabiEtt@4 Degrades abiotically: H2NCONH2+ H20 C02+ NH3 De@radation rateat 20*C Degradationrateat 2*C Concentrationindomesticsewage 16.9 mg'/i-jhr 3.3 mg/L/hr 2 to6 mg/L Bacteria(Pseudomonas putida) Popul.Gmwth lnhib. Green Algae (Scenedesmus quadticauda) 7-dayStaticPopul.Growth lnhib. 8-day StaticPopul.Growth lnhib. >10,000 mg/L (Verschuerenp 1178) 10,000mg/L (AQUIRE 2; 1980) 10,000 mg/L (AQUIRE 2; 1'978) 34 Alldegradabilftdyata are from Verschueren p 1178-9. 35 The toxicitdyata are a partiallistinogf32 testson 17 speciesfrom the EPA AQUIRE database. -29- Blue-greAelngae(Anacystaiesruginosa) 8-dayStatiPcopulG.rowthln'hib. 47 mg/L (AQUIRE 2;1978) Protozoa(Entosiphosnuicatum) 72-hrStaticPopul.Growth Inhib. 29 mg/L (AQUIRE 2;1980) Protozoa(Chilomonasparamecium) 48-hrPopul.Growth lnhib. Water Flea(Daphnia) 2,683 mg/L (AQUIRE 4; 1980) .24-hSrtaticLC50 10,000 mg/L (AQUIRE 2; 1977) Creek Chub (Somotflusatromaculatus) 24-hr LCO 24-hr LCIOO EnvironmentalAssessment* - 16,000 mg/L (AQUIRE 2; 1952) 30,000 mg/L (AQUIRE 2; 1952) Utemturedata indicatetshatthismaterialshows insigrdficant'toxtioc'iDtayphn!Afish,and green algae.Furthermore,itdegrades readilyand ft.mostlikelwyould notbe a problem in the product. However, itdoes show toxicittyoblue-greenalgae,and the low toxicittyogreen algae should be verifiebdy. ou rown testsifitistobe used inthe productathighconcentrations. Recorhmendations forAdditionaTlesting: None. 19.TritonX-305 GAS # 9002-93-1 (PRISM shows TritonX-305 to be 70% thismaterialwiththe .-Compositioonfthe remaining30% not given). Synonyms: Poly(oxy-1,2-ethanediaytlp)h,a-[4-(1,1,3,3-tetraMethylbutyl)phenyq- omega-hydmxy-polyethyleneglycolp-1,1 3,3-tetramethylbutylpheneytlher Octoxinol . P-tert-octylphenoxypotlhyoexyethanol Vendor: Roh.M & Hm Company36 36 Independence MailWest, PhiladelphiaP,A 19105. Phone: 215-592-3000. -30- Toxicily37s Bluegil(lLopomis macrochirus) 24-hr StaticLC,, 24-hrStaticLC,, 24-hrStaficLC,, 24-hrFlow-throughLC, 96-hrStaticLCao 96-hrStaticLC5. 96-hrStaticLC5. 96-hrFlow-throughLC5. 6-day Flow-throughLC50 3.5 m.g/L(AQUIRE 1;1975) 16.2mg/L (AQUIRE 1; 1975) 1,080mg/L (AQUIRE 1;1975) 10.0mg/L (AQUIRE 2;1975) 2.8mg/L (AQUIRE 1;1975) 12.Omg/L (AQUIRE 1;1975) 531 mg/L (AQUIRE 1;1975) 10.Omg/L (AQUIRE 2; 1975) 9.6mg/L (AQUIRE 2;1975) The reasonforthe highvaluesforthe 24-hrStaticLC5. (1,080mg/L)and forthe96-hr StaticLC, (531mg/L )arenotknown. The vendorforTritonX-305 (Rohm and Haas) has no data on the-aquatitcoxicitoyr biodegradabiliotfyTritonX-305. However, they are.sendinga datasheeton the aquatic toxicitoyfTritonX-100.TritonX-100 has a lower molecularW**Gightthan X-305 and they believethatthetoxicitoyfX-305 islessthan X.@l00 because X-305 islesssurfaceactive. TritonX-305 isnot.believedtodegrade biologicallRyo.hm and Haas has recentlysoldtheir Tritonproductlineto Union Carbide (1-800-752-1827)and willbe transferritnhge business in-thenear futureT.he CAS number givenby Rohm and Haas forthe seriesis9036-19-5. The followindgata isfrom AQUIRE on thisCAS number. CAS # 9036-19-5 Rainbow Trout(Salmo gairdnen) 48-hr StaticLC5. 96-hr StaticLCso 7.2mg/L (AQUIRE 2;1978) 7.2mg/L (AQUIRE 2;1978) Marine PolychasteWorm'(S6olelepisfuliginosa) 48-hrStaticLC50 96-hrStaticLCso 17.0mg/L (avg.of7.points) 13.7mg/L (avg.of8'points) (AQUIRE 2;1978). 37dTahteabtaosxei.citdyata are a partiallistinogf 13 testson 5 speciesfrom the EPA AQUIRE 38 The toxicitdyata are a partiallistinogf36 testson 5 speciesfrom the EPA AQUIRE database. -31- MarinPeolychaeWtoerm (Csotelciaapitata) 48-hrStatiLcCao 8.2mg/L (avg.of6 points) 96-hrStaticLCao 6-.0mgAL (avg.of8 points) (AQUIRE 2; 1978) AquaticSowbug (idoteabalthica) 48-hrRenewW LCso 96-hrRenewal LC, Eri ironmentalAssessments 10.0mgA. (AQUIRE 3; 1972) 5.0 mg/L (AQUIRE 3;1972) Both CAS numbers have a limiteadmount oftoxicitdyata availablewhich shows moderate toxicityS.inceTritonX-305 isa majorcomponent ofthe CE products,we willneed a complete setofdata on thismaterialinorderinordertocorrelattehe effects.otfhe CE productstotheirformulation. Recommendation a forAdditionaTlestingBOD, COD, algae,DaphniA fish,and Microtox. -32-