Document mb9O80qx1emjGK4Bx30a5MOzJ
Wildlife International, Ltd. "
Project Number 4544-102
PFOS:
A
`WITH
T96H-EHFOAUTRHSETAATDIMCIANCNUOTWE
TOXICITY
(Pimephales
TEST
promelas)
ADDENDUM
`WILDLIFE INTERNATIONAL, LTD. PROJECT NUMBER: 3M LAB REQUEST NO. U2723
US. EOnvPirPoSnmeNnutmanblaePrro8c5e0ct.i1o0n7A5gency Series 850~ EcologicalEffectsTest Guidelines
(OECD Guideline 203
454A-102
`Thisstudywasreportedusing atestsubstancepurity of 90.49%. Thetestsubstance
rep`cqrheuaverisaoctutesedlryitzhpaartteitsonehwnateesdrsweelvreibessireomednpvliys9 ecdbo/ arsaetnd7 edatnhbte/ yhphuer0 miorteyatw0 craoescfotndheptuterrwtmyipannuteaordlyebsee8s6..3T9ha%e.trouTthofesSp0o.9n6s0o3r fNroomatotermpitgwarisaunmdaaadtleatAo rsecualcchu,lamtienaonralytiocrasl dsutandoarrdoucnodnicnegntmraatyiobnes oprremseeanst.uArtetdaccohnecenotrnaetions
revisedsumpm ageafrr omty he final report andthemostrecentcertifofiacnaalytsies.
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Wildlife International, Ltd. 2.
Project Number 544-102
REVISED STUDY SUMMARY BASED ON A PURITY OF 86.9%
So
Comer
SPONSOR'SREPRESENTATIVE: Me, Susan A Besch
LocATioNF sTuDY, Raw
F[DOAATALARNEDFOARCT,OPYOFTHE
fon, Wildlife InternaMtaryliandon1al6L0t1d.
LLTD.DFLROIRECTTEMORMABTEORUT
asia
`TEST SUBSTANCE:
PFOS (Perfluorooctane Sulfonic Acid Potassium Salt)
STUDY:
CMOENARNIMTERAASTUIROENDT,EST Testpares:
LovomorTest: TORR SOURCE OF TST ORGASMS:
AGEOFTESTORGANS: NMEEGAASTRIVEEVECoNNTTSROOFL 1F0s:
wTEOoTHATL(L9NGT (nm). SERIES ---- CNONoNO-CMoEOnNRsTTERARALVTIEITDOYENFC.FOENCCTE.NTRATION:
PFOS: A 96-Hour Static Acute Toxicity Test with the Fathead Minnow (Pimephales promelas)
Negative Control, 32, 54,91, 16 0d 27mga.1.
BEixopleorgiimceanltTalemSinati-oFnear-uFaterbyps1a5y, 1139,991999
ExperimentalTermination ~February 19, 1999
|
96 Hows
Taead Vimo repiales roman)
Wilde Intemational Lid. cultures
`Easton, Maryland 21601
I
Joveites
MMeemn==03536 RaRnanggee=s0i201g100049 Trai 77 1mg4 a
32mgai/ll
32mgain
Wildlife International, Ltd. 3
CERTIFICATE OF ANALYSIS
Project Number 4544-102
}
INTERIM CERTIFICATE OFANALYSIS
4
Revision 1(3/7/00)
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E Doon 3. Theoretical Value =60%
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5. 54.1 wt/wt%
conmorsa
Page 1003
CeIntNreTAEnaRlyItiMcalCLEabRorTaItoFriIesCCAOTAEROefeFreAncNeA#:LY02S3-I0S184 DateofLastAnalysis: 08/31/00 Expiration Date: 08/31/01
Storage Conditions: Frozen 0-10C Re-assessment Date: 08/31/01
F"lPuuorriidt=ey, 01.050%9%-+(NsuMmRofimmpuertiatlieism,pu1r.i9ti3e%s,or1g.a4n5i%c+aLciCd/iMmpSuriimtpiuersi,ti0e.s,383.%A+1P%+OIAnAo,rganic
0.33%)
ToPtuarliitympu=ri1t0y0f%ro-m1all3tes.t=s0=8691.39.%%09%
"Potassium is expected in this salt form and is therefore not considered animpurity.
"oPbusreirtvyebdyfDorStChisissgaemnpelrea.lly not applicable to materialsoflow purity. No endotherm was
"iSnuolrfguarniicn atnhieosnammpeltehoadppceoanrdsittioonbse. c`oTnhveeratneidontoreSsuOl4taagnrdeehsewnecleldweittehcttehdeussuilnfgutrhe doenttehremirneastulitosn, itnhethSeOe4leimsennottalcanoalnysiss,ialednnidemipnrugrceiotndyf.idence to this interpretation. Based
"THFFABA NFPA PFPA
`THreipftlaufolruooarcoebiuctyarciicdacid Nonofiuoropentanoic acid Pentafluoropropanoic acid
"Theoretical value calculations based on the empirical formula,CsF1/SOyK* (MW=538)
`Thisworkwas conducted under EPA Good Laboratory Practice Standards (40 CFR 160),
conu30184
Page 203
CeIntNreTAEnaRlyItiMcalCLEabRoTraItoFriIesCCAOTAEROefFereAncNeA#:LY02S3-I0S184 LC/MS Purity Profle:
Ncuortvee:s,`rTehsepeCc4tiavneldy.C6TvhaeluCeSsvawleureewcaalscculaaltceudluastiendgutshiengC4theaanvdeCra6gsetraensdpaorndscealfiabcrtaotrison favreormtahgeeCre4spaonnsdeCf6acsttorasndfarrodmcutrhveesC.6 LainkdeCwi8ses,tatnhdearCd7cvuarvleuse.was calculated using the
Prepared By: Reviewed By: DScaivenitdis.t,BCeelnltre Analytical Laboratories Date
LJaobhonrFaltaohreyrtMyanager, Centre Analytical LaboratoriesDate
COA3-018A
-
c----------------
Page 3 of3
ilCOdTlOiNfICeOLIOGnYt&eArNAnLYaTtICiALoTnESaTlIN,G SLERtVIdCE.S
September 27, 2000
3MMs.SCuosrapnorAa.tioBneach 9En3v5irBounsmhenAtvaelnuLaeboratory P3.M.BBuoilxdi3n3g321-35.09
St. Paul, Minnesota 55133
Dear Susan:
454A-10E2n,cl4oSs4eAd-p1l0e3aAse, fSi4nAd-A1d0d4,en4d5u4mA-s1f0o5r,W4iS4dA-e10n6t,e4r5n4stAi.o1n0l7,Li4d5.4AP.ro1j0e8ctanNdum4b5e4rAs140S54A-101, 16yo0u have any questions, please etmknow.
Sincerely,
EKRrDpmvann
KurtR Drotar
Senior Biologist
E55r%anCoOmfmercGerDrrievem-sEansteoIngAM~a1r4y2aAnAdN21a60e1 TTNeol 4e0-8e22.r860T0o- FOoxS4E10.82282.0F6a32cO-ExTml cHctonEwmirdseinpernoaotnscsom