Document mKx0ODe91JJL9go8YNgnMYQQ

ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may alsobe referredto as PFOS, FC-95, oras a component of FX-3106. (1Octanesulfoniaccid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was 015G. The testsample is FX-3106, PO# S5233-004, EAI 85548, Sumitomo 3M Japan. Current informationindicatesitisa mixtureof2.31% PFOS, 2.6% diethyleneglycol butylether,83.38% water,10% ethyleneglycol,1.36% Sultonefoamer,0.2% xanthan gum, 0.1% 2-biphenylols,odium salt,tetrahydratea,nd 0.05% benzotriazole. The followingsummary applies to a mixture with incompletely charactedzed concentrations of impurities.Data may not accurately reflectthe toxicityof the fluorochomical component of the testsample. METHOD: Method: Not given. Type: Acute static-renewal GLP: No Year completed: 1985 Species: Pimephalespromelas Supplier:FattigFishHatchery,Brady,NE. Analyticalmonitoring: Temperature,pH, and dissolvedoxygen. Exposure period: 96-hours Statisticamlethods: Not applicableN.o mortalitoybserved. Test fishage: Juveniles Length and weight: Average length 33 mm Average weight 0.27g (wet) Loading: 0.45g/L Pretreatment:None Test conditions: Dilutionwater: Carbon-filterweedllwater Dilutionwater chemistry: Hardness: 260 rng/Las CaC03 Alkalinity: 220 rng/Las CaC03 Conductivity: 450 pmhos/cm Lighting:Not given. Stock and testsolutionpreparation:The testmateriawlas added directly tothe dilutiownater on a weightfvolurnbeasis. Concentrations dosing rate:Solutionrenewal duringthe study. Stabilitoyf the testchemical solutions: Not noted. Exposure vessels: 4 L glassbeakers containing3 L testsolution. Number of replicates:2 Number offishperreplicate5: Number ofconcentrationsf:iveplusa blankcontrol Waterchemistryduringthestudy: pH range(0-96hours): 7.8- 8.2(contreoxlposure) 7.9- 8.2 (1000 nig/Lexposure) Temperature range (0-96hours): 20 OC Dissolved oxygen range (0-96houm): 6.9- 9.6 mg/L (controelxposure) 6.6- 9.6 rng/L(1000 mg/L exposure) RESULTS Nominal concentrations: Blank control,10,50, 100,500, and 1000 rng/L Element values: 96-hour LC5o = >1000 nig/L Element values based on nominal concentrations Remarks: Testingwas conducted on a mixtureas describedintheTest Substance rrmarks field.The valuesreportedapplytothatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS The testsample 96-hour LC50 forMthead minnow was determined to be >1000 rng/L,the highestconcentrationtested.No mortalitoyr sublethal effectswere observed inthisconcentration. Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking= 2.This studymeets the criterifaorquality testing.However, thestudy lacksinformationon purityofthe testsubstance and analyticaclonfirmationofthe amount offluorochemicalproportionin solution. REFERENCES This studywas conducted by the3M Company, EnvironmentalLaboratory, St.Paul,MN, Lab Request number C2282, 1985. OTHER Last changed: 6/26/00 ENVIRONMENTAL LABORATORY FISH TOXICITY DATA SHEET DEFINITIVE (FULL-SCALE TEST) LRS_FZ4Ea. TYPE TEST AVG. FISH q(o TEST A-cxt-@e- MATERIAL F-A-310b Fbr.S5a-s3-cot TESTEDL-AT- '95.5t9 Svm;+G*w, 3/4 Jafkxn - LOT-0 IS (-r Fait Fk..I,t4.ktc6 ORGAN ISM -Fath etA @inn-bu-, SOURCE ,Aiihc4svc,.t *7DATE RE C -D-1--- -,I@ - S,57 WT.C.,;t7 g rams AVG. SIZE 3. 3 m NO. ORGANISMS PER DOSE-15- OADING BKTCH NO--ai6ls- FACTORO-q-5-g/l DISEASE TREATMENT- V on-0- TEST TIME SOLUTION VOLUME-3 LITERS/ALL-GLASS TANK STARTED9-'1-5 A,41-DATE STARTED-10-,R9-eS- DILUTION WATER INVESTIGATNR QARBON-FILTERED WELL WATER (TEMP Lc 50( 95 % TANK DIMENSIONS: 4 LITER PYREX@GLASS DATE COMPLETED- OC., D.O.-3.6 p IPM. PH-,q. ;a CONFIDENCE LIMITS)---,@//)/@/) L BEAKER Tank No. T- Initial Conc. mg/l CONTROL Total Amouunnt Addedd t 24 Hours Total No. pH D.O. cDead PPM SZ 7.1 48 Hours Total No. pH D.O. Dead PI)n 7 1 7.3 72 ours 96 Hours Total No. pH D.O. Total No. pH D.O. Lead PPM Dead IPPM o g-c 7.4' :30 0 i-,@ So /,so 0 1-3 /Cc 3 C'v Soo 1 @?ooo C, 9.t 17.a g.,1k.2 0 I k,-7 C' 2.1 7.4 0 1 7.7 0 1'7.27. P, 0 7.1 L.q 0 7-1-t-q 0 7.lixg7- 0 7.1 7, *1 0 9-a "71. 8.1 J. @ r) 7q 0/ 9.1t.q 9.1 L-q 9,0 k-7 C2 12 Test nTTMe-m-pC-.C' OC c@o [@nitials & Date L@o4g ic -30 -S,5- SA-8 10-31-95- -CAR )I- 1- -q@S' COMMENTS:,@--TnCA,V& kauo.k - Lteto@.k+s. "rni'+io-k sa)4 a-f hta @,e,@+ +,e,-4-j Te6te-heini,ki i-%,% Ytlk,.Y, -Vee-my S.4%xroie SECONDARY EFFECTS (OTHER THAN MORTALIT loco Vim Tank No. '7CONTROL Symptoms or Reactions 24-Hr. ma It u;,m4wc@.A;L", =- 479 W-tempLlr4i.%,Ce EC 50 Total Number Affected 48-Hr. 72-Hr. 9-Hr. L /-,5 1 \N.- 1)P-Lk!0, ENVIRONMENTAL LABORATORY FISH TOXICITY DATA SHEET DEFINITIVE (FULL-SCALE TEST) L R. 14 4 c.uAf- F$--3106 POOS-5;t33-CO4 TYPE TEST P%e]XU-Ykl SiO,-t @C TEST ORGAN ISM-Fai-keaA MATERIAL TESTEDtA]:85'SVS Sulnl-'Icr3"1D4 34MPan F-,t-4f%ra-i,.i6k,"c, SOURCE --Mr*Av. Ajehre.-.kc6c,LATE REC-D-1-d.4-Rs- LOT 0 15 C7 BATCH NO.,3as- AVG. WT,@nl-.2,7 grams AVG. SIZE-3-1 M NO. ORGANISMS PER DOSE- '57 OADING FACTORO- FISH DISEASE TREATMENT- k@,OV11P- TEST SOLUTION VOLUME 3 LITERS/ALL-GLASS TANK TANK DIMENSIONS: 4 LITER PYREXOGLASS BEAKER TIME S TARTE 4hi - DATE S TARTE D- 10 -,P DATE CONPLETEI)- IN DILUTION WATE QARBON-FILTERED WELL WATER (TEMP AV OC., D.O. ppm, PH-,9 , q INVE LLGATO a.3.= -@sieLe-4 LC 50(95% CONFIDENCE LIMITS)- %@/s Tank InitialTotal 24-Hours 48 Hours 72 ours 96 H ouils No. Conc. AinountTotal No. pH D.O. Total No pH D.O. Total No. pH D.O. Total No. pH D.O. mgll Added Dead PPM Dead PPM Dead PPM Dead pprq CONTROL 1 7-1 (11 S,c 7.o 0 lql 3o .7 5,0 /50 -3 loo 3oo 0 9-1 7.1 -7 7 0 9-A 7,' D 71.7-t 0 77 7.,q C C) 7,,l 7,6 C C) 7.1 7, 9.Pa, 7.1 0 g.i 0 0 -Iqly 7,q 0 3-1 7,6 C) 7,1 7. 0 Test Temp. OC C) C) [@nitials& Date COMMENTS: VlaUdi Luelghl-c,. T,e-.4c-@,ernic.&iA,-a @o ite-w-fc4x'@m1j I'ne'it'&l pt4 o ec-4Ce,Qc- le feA- 14DCCA6 '6' @y% Loixt-er, i-n SECONDARY EFFECTS (OTHER THAN MORTALITY) Tank No. Symptoms or Reactions 24-Hr. EC50 Total Number Affected 48-Hr. 72-Hr. 96-Hr. CONTROL o 2-10-85 E4VIRONMENTAL LABORATORY FINAL REPORT LAB REQUEST NO. C2466 .QUESTER NAt4E: RRR 'PT: 0222 @OJECT NO: 06 kTE RECEIVED: 12-02-85 ;'SC: NOVE.4BER WELL WATER CHARACTERISTICS CCNTRACT LAB: CONTRACT LAB COST: 0 3M E-L HOURS: 2 EST CMPLT DATE: 12-09-85 DATE COMPLETED: 12-11-85 KMPLE DATE 1 11-25-85 2 11-28m85 CODE DESCRIPTION TEST RESULT ---m ---------------------m-@----------- WELL HATER COD STANDARD PLATE COUNT TOTAL COLIFORM TOT FLUORIDE (DISTILLED) TOTAL ALKALINITY (PH 4.5) TOTAL HARDNESS TOTAL RESIDUAL CHLORINE AMMONIA NITROGEN - AS N PH SPECIFIC CONDUCTANCE TOTAL SOLIDS <094 MG/L 154 COL/NL NEGATIVE <0.4 MG/L 220 MG/L 260 MG/L <0*2 MG/L <Oo4 MG/L 8.0 450 UMHCS/CM 290 NG/L DI BATER STANDARD PLATE COUNT 1 COL/@14L TOTAL RESIDUAL CHLORINE <0,2 MCIL PH 6.7 SPECIFIC CONDUCTANCE 1.3 UMBCS/CM CONTRACT LAB SUBMITTED BY DATED