Document mBvnM5ZojwjoNodw6V22yVvYg
MICROBICS MICROTOXO TEST SUBSTANCE
TOXICITY TEST
ldentfty:Perfluorooctanoiaccid,ammonium salt;may alsobe referredto as PFOA ammonium salt,Ammonium perfluorooctanoateP,FO, FC-1 16,FC-126, FC-169, or FC-143. (Octanoicacid, pentadecafluoro-a,mmonium salt,CAS # 3825-26-1)
Remarks: The testsample isFC-143. It'psuritywas notsufficiently characterizedt,hough currentinformationindicatesitisa mixtureof96.5 100% testsubstance and 0 - 3.5% C6, C7, and Cg perfluoroanalogue compounds. The 3M productlotnumber was 427.
METHOD:
Method: MicrobicsMicrotoxig"BASIC" Procedure Test type: Staticacute
GLP: No
Year Completed: 1996
Species: Photobacteriumphosphoreum
Analyticalmonitoring:pH and osmoticcondition.
Exposure period:30-minutes
Statisticamlethods: EC50 valuescalculatebdy statisticlailnear regressionprogram providedforMicrotox@
Test organism source: Microbics'Reagent.
Test conditions:
Milli_QT.
Diluent:Millipore
water
Test temperature: Not noted.
Stock and testsolution preparation:A .primary2000 mg/L stock solutionwas preparedinMilliporMeilli-Qwater.The pH ofthe stock
solutionwas then adjustedfrom 5.8to 7.6 using 1.0N NAOH and an
osmotic sadjustmentwas made using200 mg NaC[ dissolvedin10 mL
stocksolution.Appearance oftestsolutionswas noted as "clearand
colorless"A.lltestsolutionswere made by proportionadlilutions.
Exposure vessels:3 mL Cuvettes. Number of replicates:two
Number of concentrations:Four plusa blankcontrol
Element basis: Percentlighltoss
RESULTS
Nominal concentrations: Blank control,125,250, 500, and 1000 mg/L Element value: 5-m inute EC.50= > 1000 mg/L
15-minuteEC50 = 800 (790 - 820) mg/L
30-minute EC50 = 730 (630 - 850) mg/L
Element values based on nominal concentrations.
CONCLUSIONS
The FC-143 30-minute EC50 forPhotobacteriumphosphoreum was determined to be 730 mg/L wotja 95% confidenceintervaolf 630 to 850 mg/L.
Submitter: 3M Company, Environmental Laboratory,P.O. Box 33331, St. Paul,MN, 55133
DATA QUALITY
ReliabilityK:limischranking2. Testingmeets allcriterifaorquality testingb,utlacksanalyticaclonfirmationoftestsubstance concentrations. There isa lackofcharacterizatioonfthetestsample.
REFERENCES
Thisstudywas conducted by the 3M EnvironmentalLaboratory,St.Paul, Minnesota,Lab Request number P1626.
OTHER
Last changed: 5/25/00
ENVIRONMLWAL
LABORATORY: MCROTOX
TESTSUBSTANCE:
-PC--lq5 LCT Li
PREP SHEET
LR. Date: q -;tq -9G AjWyst: -i
1.PreparatiofnStockSolution .too
MasilVoLTatSubstance., *FinalDiludonVolume.- 2
Diluent fy\,. c:@ k.0 c
FinalConcentrationof StockSolution:
9# zd# -c?16 t S
2.pH Adjustment of StockSolution
InitialPHOfStockSolution: S.S'
pH A djustedto.* *pHAdjusredwilh:
7 4. 1 eirne I.Ow
3.Osmotic Adjustment of StockSolution
Osmodc Adjuster. Comments.*C-L .1ct
K),,-c-I n,4,oc-4 7,5
4.Appearance of pH an-d@'Osmotic@alAldyjusted Stock Solution or OP.4QET.',.(.c'i!rcloene),
r_olor.
6m
'Xo
FIL.E Lot 427
MICROI'OX DATA REP@'CIR't'
Basic Test
Test Ti.me: 5 atirlutes
NUMBER.. Control
I])/!T ------------
4,i
CLINC. CP. t'i
. AitiL!StfYiei)t-.CNla
CR/64MMA
Lous
I 1 4*
@4/ EIS. 79. ('5
i
44. -!4
;AC, r 4' 14
Li
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;kc,.-4
xI Ti-@;T 1),-4T[--.
3 7=.
At
Cot,=
T -ON
.74
Bast(:
9 1(,
ii:)/ri EiC)
&.it;,/7 @2
curql- - - -- - - - - -
CR/GAMMA
of, Lct,
91 I'@, 0
-
1) 94 1 4
-
1 E;
C)
0.190 o.431 1).41 C- 4
1 f, St-2@'t. 1
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1
F)ATE TIME
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'MAT. I '-'EQUA i iL,14"L1rA F-t
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in;
FILE FC-143.K30 F'l6:76 Lot- 427,
MICROTOX DATA REPORT Basic Test
NIJMFJER -------
Control
ILI/IT -------
96.67/ 93.76 25/ 91.06
34/ 6@.10 82.Si
Ell E)7,/
6*,. 17
6@
98.51)/ 66.c,4
7
91;@.64 / *@-,. .-@,
5
91.9'.'/
F, TIC,!, for
------
T1
A 3
'r 4r
Cq lz L@L *A(.I
Ski
'3%9
a
'3 5 c,
rwq
4q
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