Document mBNjK59QL9jR6ZeOY0M5MXmX0

Wgs^%i:B%es ai;j%{;aa_^.ii6i.i.-i.,;;it AR226-2832 ^^j^^ai^s^88i%sai%^^ gi^wii^pg^afe^g^aKSSB^^.l^lSf;?* te'l^^i^^'^wtA^^-E'-iiaifi^iaeso^c..u^ai - -- ?A^tn^y.-b?^TM^-W^J",;"--.^'^r'&^^ ^ S ^ i ^ ^ " ^ ^ ' ^ ^ SMSsNg,^sSsEg^t s@Saai^!sSigc^^^^^ai ai ^iRSi a^@ 8^i p^^ ^ ^ -..^-^.^^?.^5,^^'r^^_'^^^^^^^^;^^^^^i^i^^^^w.)^.^^:^:^.^'.^^^^^^^^ aSBBE6?^^'S!?'sS;S;ii?s^S^^^^^^g?s^^???^;^ sff^s^i^^s^^^^^aKffi^^aa^;^;^;^^^;^^ .,,.- Sgiate^Mgfe^as^^ l^^^^^lglglglQIII^^^II^^ l i i ^ i i a ^ s i ^ i^ i ^ ^ ( W^.Ts^^^^^y^a.s^.^Way-a^^i^B?.?"^^;^^"^'?^^ gss?< ^'a,yS:'.i.s'^g^'lS\'^/;g'?^-S^S^^B-N*^^^'^iS^S^^ig^if'^i^t-ife'^aSifc^foset^aasa Si!?;^';;^^*8^ aSssn!:! !,.^s;5;^-;:!a!p!%(><i.^>lSil^i '>V-''^-^i;:'l-^--^.^^^ ..,.... ..,,..,.,:.,^v^^FStS<%'w^%^:S:';: ,^,,-,.-.-^''^C^i'^^ ^?;':"?^^^^A^.^^^"K-?'&vr'i^-'s-^?^2^7^S:^::^^f?^S^-'s^SWS^s^'vfS^'iS^&vf'S^^ NINETY-DAY FEEDING.STUDY IN RATS AND DOGS WITHi Haskell Laboratory Report No. C 68-73 3 company Sanitized. Does not contain TSCA CsSI; i> NINETY-DAY FEEDING STUDY IN. EATS AND DOGS WITH^ Haskell Laboratory Report No. 68-73 Report by: Bfenry Sherman' Chief, Oral Toxicology Section Approved for Biochemistry: ^j^l^ (j'C^WU^ // ^ohn R. Bames Cpief, Biochemistry Section Approved for Pathology: ^unuj/^- ,/r//^^-^ s- ^. -'1-7 / / / /' James G.- Affo^mis / Chief, Pathology Section Approved by: ^3^^^^z^ /=" /fe^t/ra^^/Charles F. Reinhardt Assistant Director HS:dhg Date: February 23, 1973 ^pany Sanitized. Does not contain TSC. NINETY-DAY FEEDING STUDY IN. RATS AND DOGS WITH Haskell Laboratory Report No. 68-73 SUMMARY I^UI^HUhas been fed to rats and dogs for ninety days at dietary levels of 0, 500, 750, and 2,500 ppm. No nutritional or clinical signs of toxicTty were observed in rats or dogs on any of the test levels. Js receiving the intermediate and highest dietary levels of showed lower erythrocyte counts, hemoglobin concentrations _ knd hei crits than those of the controls; those receiving the highest dietary level proteinuria. also Male showed a slightly higher incidence of rats receiving the two higher dietary hleevmealsturiafalBnd^_I_^_I showed slightly larger liver and kidney weights and higher organ/bodyweigh ratios than did the controls or other test group; all female test rats had higher kidney weights and kidney/body weight ratios whereas the female rats on the highest dietary level also had greater liver weights and liver/body weight ratios. Of all the organs examined histologically.on^^he livers, and only changes, those from the and these were male animals receiving 2,500 considered to be reversible. PP^'^^I^IB^ HH* showed Dogs receiving the'highest dietary level offlUB^had lower erythrocyte counts, hemoglobin concentrations and hematocrit values than did the controls. Alkaline phosphatase activities were elevated in the dogs in all test groups, whereas cholesterol values were higher in dogs receiving 750 and 2,500 ppmfi----^HH The livers of the dogs from all three test groups were neav^r than those from the control dogs. The liver was the only organ of those examined that exhibited histologic changes and this was confined to those coming from the highest level group. Thus, the results of this ninety-day feeding study and those of the previous one support only 100 ppm as a "no-effect" level forj^^^^^^^^ company Sanitized. Does not contain TSCA Ci C NINETY-DAY FEEDING.STUDY IN EATS AND DOGS WITH Haskell Laboratory Report No. 68-73 This feeding study was performed by Mrs. Kathleen t: Carroll under the direction of Dr. Henry Sherman. The clinical laboratory and biochemistry tests were performed by Mrs. Louise L. Adams, Mr. Norman W, Henry, III Mr. John R. Pennington, Jr., and Miss Adele M. Pochomis under the direction of Dr. John R. Barnes. Gross pathology and preparation of slides were carried out by Dr. Rudolf .. Culik, Dr. K. P. Lee, Mr. August H. Stenholm, Mr. William I. Swan, Mr. Francis Ulmer, Mr. Anthony T. DiLorenzo, Mrs. Jean A. Houck and Mrs. Joan A. Dimeler under the direction of Dr. James G. Aftosmis. Histopathologic evaluation of the tissues was conducted by Dr. Edwin F. Stula under Dr. Aftosmis1 direction. company Sanitized. Does not contain TSCrt Cf NINETY-DAY FEEDING STUDY IN. EATS AND DOGS WITH^ Haskell Laboratory Report No. 68-73 INTRODUCTION jhas low acute oral toxicity for the male rat, its approximate Lethal Dose (ALD) being greater than 11,000 mg/kg of body weight. The compound produced an increase in the liver weight of the animal do-sed a"t 11,000 mg/kg and possibly in the animal dosed at 7,500 mg/kg. The compound shows high cumulative oral toxicity. When administered to each of six rats at a dose of 5,000 ing/kg/day, all died after having received three to five doses. When administered to rats at a dose of 2,250 mg/kg/day, it produced heavy livers in animals sacrificed after the tenth dose with evidence of some recovery when the animals were sacrificed after a 14-day recovery period. The histologic changes observed in these large l:^er^werenot^_hose, ^^mUHI^^m^ usua^^assoc^atfid with degenerative changes jUHIUBU^L Repeated doses of 220 or 22 mg/kg/day did npc produce^nv evidence of liver enlargement or pathology H^^----K& A ninety-day feeding study in rats and dogs has already been conducted J^II^^H^ Both species were fed, for 35 days, at dietary levels ofU^n)0^5QO, and 2,500 ppm; after this period of time, th-e 500 ppm and 2,500 ppm dietary levels were doubled to 1,000 ppm and 5,000 ppm for the duration of the study. Compound-related'changes were observed primarily in the liver of both species of animals at the intermediate and highest level of feeding, i.e., 500-1,000 ppm and 2,500-5,000 ppm; these included gross changes and increased liver weights. Histologic evidence of hepatic changes was observed only at the highest dietary level, i.e., 2,500-5,000 ppm. considered the "no-effect" level. Thus, 100 ppm was In -order to help obtain broader FDA sanction to 'use^BjUJBjon paper products in contact with food, the present ninety-day feeding study in rats and dogs was undertaken in an attempt to determine whether a higher "no-effect" level than that obtained earlier existed. TEST MATERIAL The test material was supplied by the Organic Chemicals irtment as{~ company Sanitized. Does net contain TSCA CB! A. RATS f PROCEDURE \ Fifty male and 50 female weanling albino rats (ChR-CD) were housed in pairs, sexes separate, in suspended stainless'1'steel wire cages and fed ground Purina Laboratory Chow (GPLC) with 1% corn oil (CO) added. During a pre-test period of five days, the animals were observed with respect to food consumption, eating habits, and weight gain. They were divided into four equal average-weight groups of 10 male and 10 female rats each at the end of this period on the basis of weight gain and freedom from gross respiratory disorders or other clinical signs of disease. The groups were then assigned at random to receive the following diets: Group I (Control) II III IV Computer Groups (1 and 2) (3 and 4) (5 and 6) (7 and 8) - ____________Diet____------- GPLC + 1% CO GPLC + GPLC + GPLC + 1% CO + 17. CO + 1% CO + 500 ppm 750 ppm 2,500 ppm ^ The highest.dietary level (2,500 ppm) had been suggested by the FDA to act as a bridge between the present study and the one con ducted earlier under^^BBBp The test material was added as active. ingredient, |------Bl|^^^^^^1|HHHIHHII^HHI^^^^BH^^^R An amount tribute o d f waj:er by^^^ w a ^^^l^ ^^^c^HoHIn^^tHrtootlh e diet die t equ in ivalen Group t I to V. tha't conDiets were prepared rresh eac1a weeksm^stored at refrigerator temperature until used. The animals were weighed once a week during the entire study. Food consumption data were obtained on a group and sex basis at the times the animals were weighed. During the test, the animals were examined routinely for any abnormal behavior and any clinical manifestations of toxicity. Hematological, urine and biochemical analyses were conducted on ten male and ten female rats from each group after they had been on their respective diets for one, two, and three months.. Hematological evaluations included an erythrocyte count, a measure (ff hemogldbin con centration, a measure of the hematocrit, and a total and differential white blood cell count. Urine analysis consisted of a measure of the 24-hour urine volume, concentration in milliosmoles and creatinine, a test for sugar, blood, protein, and urobilinogen, and an observation of the color, appearance, and pH. Specimens with a negative test for blood were combined to form two pools of urine for each group and the sediment Srom these pools examined microscopically. All specimens with a posigive test for blood were examined separately. In the biochemical tests, to measure liver function, alkaline phosphatase and glufcamicpyruvic transaminase activities and biliruuin concentration were^1 measured in blood taken from the tails of 10 males'and 10 females in each group. %5W)ar;v Sanitized. Does not contain TSCA CKl A. RATS (Continued) PROCEDURE (Continued) After 91-98 days of continuous feeding, all the animals in each group were sacrificed by CHCIn administration. The following organs were weighed: brain, heart, lungs, liver, spleen, kidney, testis, stomach, adrenal, 'and pituitary. Organs or tissues, preserved in formalin and stained with hematoxylin-eosin, included, in addition to those listed above, the following: eye, exorbital lacrimal gland, sciatic nerve, skin, mammary gland, bone marrow, lymph node, skeletal muscle, trachea, aorta, salivary gland, esophagus, colon, cecum, duo denum, urinary bladder, prostate and seminal vesicles, uterus, Fallopian tubes, ovary, thyroid, parathyroid, and thymus. The above tissues from the control and highest level group (2,500 ppm) were evaluated histopathologically; only the livers from the animals in the other two test groups were examined histopathologically. RESULTS 1. Weight Gain " {;. , jr Average body weight curves for control and test groups of animals are plotted in Figure 1; average body weights and average weight, gains are summarized in I) Tables I,;. II, V and VI. The presence of 2,500 ppmg^^mB|in the diet of ^ male and female rats did not adversely affect their rate of weight gain. 2. Food Consumption A summary of the average daily food consumption data, computed as grams ingested per rat for each group, is presented in Tables III, IV and V. There were no meaningful differences among the control and test groups with respect to the amount of food they consumed over the entire test. 3. Food Efficiently Food efficiency data, calculated as gram weight gain per gram of food consumed, are presented in Tables III-V; There were no meaningful differences among control and test groups with respect to food efficiency. DoesnotcortalnTSCACBl eo,,p=n Sanded. RESULTS (Continued) A. RATS (Continued) 4. Dose The average daily ingestion of tes^mater^l during each week was calculated in milligrams o|IBUBH>er kilogram of body weight; these data are presented in Table VI. The decline in the average dose of test material received by animals observed in this study is normal and typical of that observed in most feeding studies where rapidly growing animals are used initially and the concen tration of test material is kept constant throughout the test. 5. Clinical Observations None of the test animals in any of the three test groups exhibited any clinical signs of toxicity during the entire test that could be attributed to the test material. 6. Mortality None of the animals in the control and died during the entire feeding study, fc ,- , ^ 7. Hematology test groups The results of the periodic hematologica lions conducted on rats fed the various levels of and their controls are summarized in Table VII, The erythrocyte count ^lemo^^bin concentration and hematocrit of the rats fedjBU^Bwere lower than those of the controls at 750 ppm and above. A one-way ^na^SL^^f variance showed that the treatment with ||^^----------haadsignificant effect on these measurements. The values for these measurements^^bgerved in the males but not the females fed 750 PP'^lHlUBIl were signifi cantly (p < 0.05) lower than those for the controls. Both sexes were affected by a level of 2,500 ppm in the diet. The calculated hematologic indices, mean corpuscular volume (MCV) and mean corpuscular hemoglobin (MCHLu.-d^atgd that the erythrocytes of the rats fed 2,500 ppmgJ^IHBwere aacrocytic and hypochromic. There were no effects on the number or distribution of leucocytes. company Sanitized. Does not contain TSCA CBi RESULTS (Continued) A. RATS (Continued) 8. Urinalysis The results of the periodic urjjxe analyses conducted on rats fed various levels of^HIH^^B^i summarized in Table VIII. A slightly higher incidence of hematurj.a and proteinuria occurred in the rats fed 2,500 ppmjI^^H than in the other groups. All other measurements and observations made on the urine of the treated rats did not differ from those of the controls. 9.- Bi-ochemi-stry The results of the periodic biochemical measure ments conducted on the blood of rats fed various levels of [are summarized in Table IX. No effect on any of the biochemical measurements wasfound that could be related to the presence of the 10. Pathology A summary of the average weights of select organs taken from the control and test groups is presented in Table X; organ/body weight ratios are summarized in Table XI. Among the test groups, Jiale animals receiving the| intermediate and high levels of|fll^^BBjShowed higher average liver and kidney weights^Doth^Kver/body weight and kidney/body weight ratios were also slightly greater in these groups. Among the female test,groups, jail showed higher average kidney weights and kidney/body weight ratios than did the controls; average liver weights and liver/body kweight ratios were slightly greater in the animals receiving 2,500 ppm]IHH^jgthan in the other test groups or the control. ^^^^^^^- ^ , , A summary of the histopathologic findings is presented in Table XII, where 0 = no abnormalities detected, + = slight degree of lesion present, and X = organ not on slide. ^ . Test chemical-related histopathologic effects were found only in the livers of malerat^^ed the highest (2; 500 ppm) dietary level otJ^f^H^U Examination of the livers from rats that received the two lower levels of the test chemical did not reveal any compound-related effects. company Sanitized. Does not contain TSCA CBt RESULTS (Continued) A. RATS (Continued) 10. Pathology (Continued) The liver changes observed in the males in the highest dietary level group consisted of fatty change, cytomegaly, and cytoplasmic hyaline droplets. This type of liver change is considered to be reversible. PROCEDURE B. DOGS During a pre-test period of approximately one month, 16 male and 16 female beagle dogs, 9 to 14 months old, were given Wayne Dog Krums and water ad libitum between 3:00 p.m. and 7:00 a.m. Animals were examined daily for any abnormal behavior and any clinical manifesta tions.^ of toxicity. During this period, specimens of blood and urine were collected from each dog twice for clinical laboratory examina tions. The tests included in these examinations are listed below: Hematology: Erythrocyte count, hemoglobin concentration, hematocrit, total and differential leucocyte count. i'- Urinalysjs: An observation of color, appearance and pH; a measure of the 24-hour urine volume, creatinine, and osmolality; a test for sugar, blood, protein, acetone, urobilinogen, and bilirubin; a microscopic examination of the sediment. Biochemistry: Glucose,urea-nitrogen, creatinine, cholesterol, alkaline phosphatase, glutamic-pyruvic transaminase, bilirubin, total protein, albumin, and albumin/globulin ratio Four males and four females were allocated to each of the four groups on the basis of normal clinical and nutritional evaluations and assigned at random to receive the following diets: Group I (Control) II III IV _________Diet____ Wayne Dog Food (Krums' Krums + 500 ppm------ Krums + 750 Ppm^H Krums + 2,500 ppmjj Diets were prepared fresh each week. Diet was offered ad libitum to the dogs between 3:00 p.m. and 7:00 a.m. ; .^water was available at all times. company Sanitized. Does not contain TSCA CE31 B. DOGS (Continued) PROCEDURE (Continued) Diet consumption and body weight data were obtained each week and calculations were_made to determine the approximate daily dose each week of^^^^l^lWper kilogram of body weight. Animals were examined daily foranycraoical signs of toxicity. The same clinical laboratory examinations made during the pre test period were conducted on each dog after one, two, and three months of feeding. After 98-105 days of continuous feeding, all dogs were sacri ficed by electrocution and submitted to gross and his topathologic evaluation. Organ weights were obtained for the following: brain, heart, lungs, liver, spleen, pancreas, kidney, testis, prostate, stomach, thymus, adrenals, pituitary, and thyroid. Organs and tissues were preserved in formalin and stained with hematoxylineosin; these included, in addition to those mentioned above, the following: epididymis. Fallopian tubes, aorta, esophagus, uterus, ovary, duodenum, cecum, mammary gland, urinary bladder, spinal cord, trachea, salivary gland, bone marrow, lymph node, colon, sciatic nerve, skeletal muscle, eye, and skin. All tissues taken from the dogs on the control diet and highest test level diet were evaluated histologically; only the livers from the animals receiving the low and intermediate levels of ^----M---->rereexamined histologically. The livers from the contro^^B^n^^ast level dogs were stained with Oil Red 0. RESULTS 1. Body Weight The weekly body weight data of the individual dogs are presented in Tables XIII and XIV. All dogs, control and test, showed normal body weight gains during the study. 2. Diet Consumption Values calculated for average daily diet consump tion for each dog are presented in Tables XV and XVI. The amount of diet consumed by each dog varied from week to week. However, there was no adverse effect by the test material upon diet intake. 3. Dose The average daily intake of in milli grams per kilogram of body weight wa 'd for each dog; these results are presented in Tables XVII and XVIII. ompany Sanitized, Does not contain TSCA CBS RESULTS (Continued) 3. Dose (Continued) ^ll^ll Siyce the body weights of most of the dogs remames relatively constant throughout the test, the dose _I------receivedby each treated dog each week fluctuated w^h^ diet intake. 4. Clinical Signs Regular examination disclosed no clinical changes in test dogs that could be attributed to the feeding All dogs survived the 90-day feeding period without incident. 5. Hematology The results of the hematological measurements con ducted throughout the feeding study are summarized in Table XIX; the numbers represent average values obtained during pretreatment and treatment periods. Individual values- are presented in Appendix I. The erythrocyte count, hemogj-obin concentration, and hematocrit of the dogs fed 2,500 ppnfH^Bfl^ere generally lower than those of the other dogs, cohrro^ana test, during treatment. A one-way analysis of variance indicated that the treatment with^^^^^^^^Bignificantly affected the hemoglobin concentration and hematocrit, whether expressed as grams of hemoglobin per 100 ml and percent of packed cells, or as the change from the pre-test observation for these measurements. 6. Urinalysis The results of the urine analysis measurements con ducted throughout the feeding study are summarized in Table XX; the nrmibcrs.represent average values obtained during the pre treatment and treatment periods. Individual values are presented in Appendix I. ^llHH No effect attributable to the addition to the dogs' diet was found in any of the measurements or observations made on the urine. 7. Biochemistry The results of the biochemical measurements conducted throughout the feeding study are summarized in Table XXI; the figures recorded represent average values obtained during the Company Sanitized. Does sioi contain TSCA CBS RESULTS (Continued) 7. Biochemistry (Continued) pretreatment and treatment periods. presented in Appendix I. Individual values are There was no effect on the glucose, urea-nitrogen, ' , creatinine, transaminase, bilirubin, or plasma protein values, A one-way analysis of variance, however, showed that the total cholesterol .and alkaline phosphatase activity of the dogs fedlBIHI^R^ere significantly affected by the treat ment. TheaUca^Se phosphatase activity of the dogs fed the lowest dose, 500 pprnHlHHUU was elevated significantly (p < 0.05). Both alKa^Kephosphatase and cholesterol values.. were elevated in the dogs fed 750 ppm and 2,500 Ppm^lBHHBl This ef-feet occurreda.ffeer one mont-h, was maximum at- two months, and then stabilized, or decreased slightly, at three months. The effect was significant for the relative change from the pre-exposure, expressed as a percent, as well as for the increase in Bessey units (alkaline phosphatase) or mg % (cholesterol) for theBU|^^^|treated dogs. 8. Pathology off^U^^re The individual organ weights of the dogs sacrificed after three months' feeding Tables XXII and XXIII. ^^^----^ presented in The small group sizes and ^.arge variation do not per mit a complete statistical analysis of the liver weights, However, a variance analysis of liver/body weight ratios indicates fha.t, there was? a difference between control and test groups with respect to this ratio, a heavier liver being the effect of treatment. This is summarized in Table XXIV, where o^UUlH '^^H mean values are recorded. It would appear that the livers of all three ^roups^^^ e., low, intermediate, and high dietai levels were affected by the presence |HHin the aiet. A summary of the histopathologic findings is presented in Tables XXV-XXVIII. The livers from the highest level of feeding were pale and had rounded edges with a tight capsule. Histologically, the liver from the dogs in the highest;level of^ feeding (2,500 ppmgljBBjj^B was the only organ affected. The liver changes were difficult to detect. They consisted of a slight enlargement of hepatocytes (hypertrophy) together with an uneven distribution of cytoplasmic particles (degenera tion) . Examination of the livers from control and high level dogs stained with Oil Red 0 did not reveal an increase of lipi.d. i5arnpanyal*J26d. Does not contain TSRA C' - 10 - RESULTS (Continued) 8. Pathology (Continued) No compound-related abnormalities were the livers of the dogs receiving 500 and 750 pp DISCUSSION In the previously-reported ninety-day feeding study in rats and dogsj------HJB the lowest level fed, 100 ppm, was considered the "no- effec?^Lever The other two levels, 500-1,000 ppm and 2,500-5,000 ppm, did produce changes in both rats and dogs. In rats, this consisted of hematological changes at 2,500-5,000 ppm, increased liver and kidney weights at 500-1,000 ppm and 2,500-5,000 ppm levels, and pale yellowish livers in some male rats at the mid and highest levels; histologic changes were observed in only the livers from the highest level, 2,5005,OOO ppm. In dogs, plasma cholesterol and alkaline phosphatase were elevated in those fed 2,500-5,000 ppm^HH^^Bl suggesting liver damage. Liver weights were increased eS^hemradle and highest dietary levels; histologic changes were observed in only the livers of dogs receiving the highest dietary level.- The present study confirms the results obtained earlier. There does not appear to be a "no-effect" level in this present study, since increased liver and kidney weights were observed at the lowest dietary level in rats and since increased alkaline phosphatase activity and increased liver weights were observed in the lowest level in the dogs. Thus, the results of this ninety-day feeding study and those, of the previous one support only 100 ppm as a "no-effect" level for^^^^^HB' ^^^^^^^^^ SUMMARY IJI^HHHihas been fed to rats and dogs for ninety days at dietary leveTsofO,500, 750, and 2,500 ppm. Mo nutritional or clinical signs of toxicity were observed in rats or dogs on any of the test levels. " " ? /' ^^_Rat^receiving the intermediate and highest dietary levels of _^^^^^^fs bowed lower erythrocyte counts, hemoglobin concentrations and hematocrits than those of the controls; those receiving the highest dietary level also showed a slightly higher incidence of hcmaturia and . proteinuna. Male rats receiving the two higher dietary levels of AHlHHphowed slightly larger liver and kidney weights and higher -organ/body weight ratios than did the controls or other test group; all female test rats had higher kidney weights and kidney/body weight ratios whereas the female rats on the'highest dietary level also had greater liver weights and liver/body weight ratios. Of all the organs examined histologically, onLy the livers, and only those from the male animals receiving 2,500 ppm^^^^^^^H showed changes, and these were considered to be reversible^^^^^^^^ Dogs receiving the highest dietary level ofHBIIH1^'1 lc>wer erythrocyte counts, lower hemoglobin concentrations, and hematocrit values Company Sanitised. Does not contain TSCAGG - 11 - SUMMARY (Continued) than did the controls. Alkaline phosphatase activities were elevated in the dogs in all test groups, wherea_cholesterol values were higher in dogs receiving 750 and 2,500 PP^fllHIHBB rltne livers of the dogs from all three test groups were heavierthanthose from the control dogs. The liver was the only organ of those examined that exhibited histol^gic changes and this was confined to those coming from the highest level group. Thus, the results of this ninety-day feeding study and those of _the previous one support only 100 ppm as a "no-effect" level for f t/"~~\ .-N' company Sanitized. Doss not contain TSCA CBI 4 5 6 7 8 9 10 il 12 13 ^-.impanySaniSizsd. Does not contain TSCA CBt TABLE I AVERAGE BODY WEIGHTS AND AVERAGE MALE EATS FED VARIOUS LEVELS WEIGHT OVI C GAINS.OF Days on Test 0 7 14 21 28 35 42 49 56 63 70 77 84 91 0- 7 7-14 14-21 21-28 28-35 35-42 42-49 49-56 56-63 63-70 70-77 77-84 84-91 Group I Control I I Group 5 00 ppm I I I lSroup 750 ppm Average Body Weight (gm) 70 123 179 . 233 266 321 358 388 411 432 452 472 487 500 69 120 174 227 280 323 358 390 413 431 451 470 482 " 497 69 119 172 229 267 320 358 396 423 441 461 478 492 510 Average Weight Gain (gm) 53 51 50 56 54 53 54 53 57 33 53 38 55 43 53 37 35 38 30 32 38 23 23 27 21 18 18 20 20 20 20 19 17 15 12 14 13 15 18 Group IV 2,500 ppm 68 117 168 221 267 310 345 377 404 423 448 471 489 495 49 51 53 46 33 35 32 27 19 2.5 23 18 6 QQWyWS sanni^"'"0"1""'8"0" TABLE II AVERAGE BODY WEIGHTS AND AVERAGE WEI, FEMALE EATS FED VARIOUS LEVELS OP :T GAINS OF Days on Test 0 7 14 21 28 35 42 49 56 63 70 77 84 91 0- 7 7-14 14-21 21-28 28-35 35-42 42-49 49-56 56-63 63-70 70-77 77-84 84-91 Group I Control I I G roup 5 00 ppm I I I (Sroup 750 ppm Average Body Weight (gm) 69 70 70 113 114 110 147 148 149 170 173 175 189 194 196 205 208 214 220 225 228 238 236 244 250 247 257 250 253 262 259 260 273 270 268 286 279 276 290 282 I'll 295 Average Weight Gain (gm) 44 44 40 35 34 39 22 25 26 19 - 21 21 16 14 18 15 17 14 18 11 16 12 11 13 0 6 5 9 7 11 11 8 13 9 8 4 3 1 5 Group IV 2,500 ppm 70 112 150 176 195 208 217 236 246 247 261 267 275 276 42 38 26 19 13 9 19 10 1 14 6 8 1 con>.inTSCACB> Company Sanded. Do.- no, TABLE III OF|B^1w AVERAG]3 DAILY FOOD CC(NSUMPTIO'N AND FOOD.JFFICI]ENCY OF MALE EATS EED VARIOUS LEVELS Days on Test 0- 7 7-14 14-21 21-28 28-35 35-42 42-49 49-56 56-63 63-70 70-77 77-84 84-91 Group I Control I I G r o u p 500 ppm I I I Group 750 ppm Average Daily Food Consumption (gm) 14.9 20.1 19.4 23.3 25.0 25.4 24.4 25.6 25.6 24.4 24.5 25.0 23.8 14.8 18.4 20.7 24.3 22.1 25.8 25.2 25.7 25.5 24.9 24.5 24.7 23.1 14.0 18.5 21.1 23.3 25.5 25.8 26.1 25.8 25.8 25.9 25.9 26.3 25.4 Group IV 2,500 ppm 14.1 18.2 20.1 23.1 24.8 25.4 25.6 26.1 24.2 26.1 27.9 27.4 25.4 0- 7 7-14 14-21 21-28 28-35 35-42 42-49 49-56 56-63 63-70 70-77 77-84 84-91 Gram Weight Gain/Gram Food Consumed . 0.51 0.40 0.40 0.20 0.31 0.21 0.18 0.13 0.11 0.12 0.11' 0.09 0.08 0.50 0.42 0.37 0.31 0.28 0.19 0.18 0.13 0.10 0.11 0.11 0.07 0.09 0.51 0.41 0.38 0.23 0.30 0.21 0.20 0.15 0.10 0.11 0.09 0.08 0.10 0.49 0.40 0.38 0.28 0.25 0.19 0.18 0.15 0.11 0.14 0.12 0.09 0.03 Company Sanil^sd. Does not contain TSCA CF^ TABLE IV AVERAGE DAILY FOOD CONSUMPTION AND FOOIL EFFICIENCY OF FEMALE RATS FED VARIOUS LEVELS 07} --s^-- Days on Test 0- 7 7-14 14-21 21-28 28-35 35-42 42-49 49-56 56-63 63-70 70-77 77-84 '84-91 Group I Control "Grou^ir"- II Group 500 ppm 750 ppm Group IV 2,500 ppm Average Daily Food Consumption (gm) 13.5 16.1 1-2.9 17.0 17.6 17.8 18.2 18.8 21.2 16.2 18.2 18.5 17.0 14.0 16.9 1-6.8 18.2 16.9 19.1 17.7 20.4 20.6 17.6 19.2 19.0 16.9 13.0 16.0 15.6 17.3 17.2 18.1 18.8 19.1 18.3 17.3 20.2 18.6 17.9 13.1 15.9 14.7 17.4 16.9 17.3 18.1 18.9 18.2 17.3 18.9 18.3 16.5 0- 7 7-14 14-21 21-28 28-35 35-42 42-49 49-56 56-63 63-70 70-77 77-84 84-91 Gram Weight Gain/Gram Food Consumed 0.46 0.31 0.24 0.16 0.13 0.12 0.14 0.09 - 0.08 0.08 0.07 0.02 0.45 0.29 0.21 0.16 0.12 0.13 0.09 0.08 0.04 0.06 0.06 0.06 0.01 0.44 0.35 0.24 0.17 0.15 0.11 0.12 0.10 0.04 0.09 , 0.09 0.03 0.04 0.46 0.34 0.25 0.16 0.11 0.08 0.15 0.08 0.01 0.11 0.05 0.06 0.01 Company Sanitized. Does not contain TSCA CB1 TABLE V AVERAGE WEIGHT GAIN, FOOD CONSUMPTION, AND FOOD EFFICIENCY DATA, CALCULATED AT APPROXIMATELY MONTHLY INTERVALS, OF MALE AND FEMALE EATS FED VARIOUS LEVELS OFB^H^----^ 9 ',, 5 Group I (Control) Days on Test 0-28 28-56 56-91 Total I]------3 II (500 PPg^^-. 0-28 28-56 56-91 Total III (750pp^^- (^^^^^^^^rf 0-28 28-56 56-91 Total IV 0-28 28-56 56-91 Total Weight Gain 196 145 89 430 211 133 84 428 198 156 87 441 199 137 91 427 MALES Food Con sumption (gm) 544 703 863 2110 548 592 859 2099 539 723 905 2167 529 713 917 2159 Food Effi ciency 0.36 0.21 0.10 0.20 0.38 0.19 0.10 0.20 0.37 0.22 0.10 0.20 0.38 0.19 0.10 0.20 Weight Gain 120 61 32 213 124 53 30 207 126 61 38 225 125 51 30 206 FEMALES Food Con sumption (gm) 417 507 637 1561 461 519 654 1634 434 512 647 1593 428 499 625 1552 Food Effi ciency 0.29 0.12 0.05 0.14 0.27 0.10 0.05 0.13 0.29 0.12 0.06 0.14 0.29 0.10 0.05 0.13 Gompany Sanitized. Does not contain TSCA CBI TABLE VI AVERAGE DAILY INTAKE OF Days on Test 0- 7 7-14 14-21 21-28 28-35 35-42 42-49 49-56 56-63 63-70 70-77 77-84 84-91 0- 7 7-14 14-21 21-28 28-35 35-42 42-49 49-56 56-63 63-70 70-77 77-84 84-91 Average Dose in Milligrams/Kilogram/Day Group I Control I I Group 500 ppm MALES 78 63 52 48 37 38 34 32 30 28 27 ... 26 24 I I I G r o u p 750 ppm 112 95 79 70 65 57 52 47 . 45 43 41 41 38 FEMALES 76 108 65 92 52 72 50 70 42 63 44 61 38 60 42 57 41 53 34 48 36 53 35 49 31 46 Group IV 2,500 ppm 382 319 259 237 215 194 177 167 144 149 152 142 129 361 303 226 234 209 204 200 196 185 170 1-79 169 150 Company Sanitized. Does noi contain TSCACBr TABLE VII SUMMARY OF HEMATOLOGIC MEASUREMENTS ON RATS FED Erythrocytes x I06/inm3 Hemoglobin gm/100 ml Hematocrit % Leucocytes x 103/nnn3 Neutrophils % Lymphoeytes % ppm 3-n Diet 0 500 750 2,500 0 500 750 2,500 0 500 750 2.500 0 . 500 750 2,500 0 500 750 2,500 0 500 750 2,500 1 5.98 5.64 5.58 14.9 13.9 13.3 43 39 38 13.4 12.9 13.7 22 26 25 75 72 73 MALES Months on Test 2 6.14 5.65 5.39 4.92 15.6 15.6 14.5 13.7 45 43 42 39 13.2 12.4 14.1 15.9 19 22 21 19 77 75 75 79 3 6.38 5.70 6.12 5.27 16.7 15.2 15.6 15.5 49 43 45 44 12.6 10.7 12.3 14.5 18 23 22 21 79 75 75 76 PR THREE MONTHS 1 5.87 5.86 5.94 15.4 15.2 14.9 44 41 41 10.8 10.2 14.4 20' 21 19; 77- 76 78 FEMALES Months on T 2 4.65 5.38 4.33 4.18 13.5 15.1 12.1 11.8 37 43 33 32 10.5 9-.-3 10.6 14.2 22 17 21 ' 19 74 80 75 77 TABLE VII (Continued) SUMMARY OF HEMATOLOGIC MEASUREMENTS ON RATS FE Monocytes 7. Eosinophils % Basophils 7 /o Atypical Cells Nucleated RBC's per 100 WBC's ppm in Diet 0 500 750 2,500 ' 0 500 750 2,500 0 500 750 2,500 0 500 750 2,500 0 500 750 2,500 MALES Months on Test 1 2 3 >' 1.0 0.9 0.1 0.6 0.6 1.5 0.4 0.1 1.4 0.5 0.5 2.0 2.4 2.5 2.3 2.1 1.1 3.2 2.8 1.4 2.2 2.9 0.1 0 0 0.1 0.1 0.1 0.1 0 0 0 0.1 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0 0 0 0 0.1 0 0 0 0 F|OR THREE MONTHS FEMALES Months on Te 1 2 0.3 1.0 0.1 1.0 0.9 0.9 1.2 2.5 4.7 2.7 2.2 3.2 2.1 3.2 0 0 0 0.1 0.1 0 0.1 0 0 0 0 0 0' 0 0 0 0 0 0 0 0 TABLE VIII SUMMARY OF URINALYSIS DATA ON RATS FE Volume ml/24 hrs. Osmolality mO s /L Creatinine mg/24 hrs. Blood Number Bositive Sugar Number Abnormal Bilirubin Number Positive ppm in Diet 0 500 750 2,500 0 500 750 2,500 0 500 750 2,500 0 500 750 2,500 0 500 750 2,500 0 500 750 2,500 MALES Months on Test 1 2 i8 15 15 1349 n 19 22 18 20 1696 1578 1760 1818 1845 1757 9.2 17.4 16.7 9.5 17.8 9.3 16.7 0 1 0 0 0 1 2 0 0 0 . 0 0 0 0 0 0 ' 0 0 0 0 0 1) Number ++ or greater by Clinitest 3 17 16 18 21 1857 2012 1860 1711 15.2 15.9 17.0 16.7 0 0 0 1 0 0 0 0 0 0 0 0 FOR THREE MONTHS 1 13 12 13 1590 1849 1783 7.1 7.1 7.4 0 0 1 0 0 0 0 0 0 FEMALES Months on 2 19 15 15 18 1473 1763 1792 1644 11.8 10.0 10.8 10.3 1 1 0 3 0 0 0 0 0 0 0 0 TABLE VIII (Continued) SUMMARY OF URINALYSIS DATA ON RATS FE FOR THREE MONTHS Protein Number Abnormal Urobilinogen Erythrocytes per hpf Leucocytes per hpf Epithelial Cells per hpf Casts per Ipf ppm in Diet 0 500 750 2,500 0 500 750 2,500 0 500 750 2,500 0 500 750 2,500 0 500 750 2,500 0 500 750 2,500 MALES Months on Test 1 2 0 0 0 0 0 0 1 0.4 1.0 1.0 0.6 1.0 0.5 1.0 0 QS) 0 0 0 . 0^ Qa.),b,) 0-1 0-1 0-1 0-1 0-1 0-1 0-1 0 0 0 0 0 0 0-1 0 0 0 0 0 0 0 3 0 0 0 2 0.6 0.8 0.7 0.7 0 0 S'> 0-1 0-1 0-1 0-1 0-1 0-1 0-1 0 0 0 0 0 2) Number -H- or greater by Albustix a) 1/10 hernaturia b) 1/10 with positive test for blood examined separately c) 2/10 with positive test for blood examined separately FEMALES Months on T 1 2 0 0 0 0 0 0 0 0.7 1.0 1.0 0.7 1.0 ^ 0.6 1.0 0 ^> S.)c 0-1 0-1 0-1 0-1 0-1 0-3 0-1 0 0-1 0-1 0 0 0 0 0 0 0 0 0 0 0 TABLE IX SUMMARY OF BIOCHEMICAL MEASUREMENTS ON RATS"FED, Alkaline Phosphatase Bessey Units Transaminase Reitman-Frankel Units Bilirubin mg % ppm in Diet 0 500 750 2,500 0 500 750 2,500 0 500 750 2,500 MALES Months on Test 1 2 3 44 38 32 - - - 47 38 33 41 33 24 18 12 8 - - - 18 10 9 18 12 18 1.1 1.7 0.6 , - - 0.6 1.5 0.3 0.5 1.4 0.4 OR THREE MONTHS FEMALES Months on T 1 2 35 22 33 23 26 20 14 19 15 17 1.0 0.9 1.2 18 21 0.3 0.4 0.4 Group (Control) TABLE X AVERAGE ORGAN WEIGHTS IN GRAMS OF MALE AND FEMALE RATS FED VARIOUS LEVELS OF( Final Body Weight Brain Heart Lungs Liver Spleen ^ Kidney Test is Stomach Ad MALES 500 2.19 1.44 2.39 17.08 0.77 3.46 3.36 2.04 0 2.24 1.50 2.40 17.95 0.71 3.78 3.27 1.90 0 2.25 1.52 2.47 21.46 0.70 4.30 3.22 2.06 0 2.19 1.45 2.42 22.00 0.69 4.45 3.31 2.03 0 FEMALES 286 2.06 0.91 1.84 10.72 0.57 2.13 288 2.02 0.96 1.76 11.39 0.49 2.66 306 2.08 0.97 1.86 11.76 0.53 2.87 276 2.06 0.97 1.85 13.24 0.53 3.04 1.43 0 1.53 0 1.65 0 1.46 0 r\ ^ TABLE XI SUMMARY OF ORGAN/BODY WEIGHT RATIOS OF MALE AND FEMALE RATS FED VARIOUS LEVELS O^ Group I (Control) II Brain 0.44 0.44 Heart 0.29 0.30 Lungs 0.48 0.48 Liver 3.41 3.55 Spleen % of Body Weight Kidney Testis Stomach MALES 0.15 0.69 0.67 0.41 0.14 0.75 0.65 0.38 Adren 0.012 0.01 III . 0.43 0.29 * (750 pprti g--------ID IV 0.44 0.29 f----1 . (2,500 ppm a 0 v-' 3 . i . I 0.72 0.32 ^ (Control) |3 II 0.70 0.33 ' III 0.68 0.32 8 | Q(7f5--0 p--pm ------Q Vi^^Q S IV 0,75 0.35 11 0pp^ 0 > 0 OB 0.47 0.49 0.64 0.61 0.61 0.67 4.10 4.44 3.75 3.95 3.84 4.80 0.13 0.14 0.82 0.90 FEMALES ---------- 0.20 0.74 0.17 0.92 0.17 0.94 0.19 1.10 0.61 0.67 - - 0.39 0.41 0.50 0.53 0.54 0.53 0.01 0.01 0.02 0.02 0.02 0.03 t) H;\SKi?LL 6 CCWP'iUNO 1 Af.;'lAL ' G:<nup S TKLAI 11ENT OAYS por-TKnAT;it:'n' DAYS OIr'T/'DSI: VALUE I INITIAL HL-IGHT {.llrl r.l-IGUi' FINAL HtlGHI Sl-X Dl^.O/^AC^tFlCFU TABLE XII H [STOP,ATHOLUI;y ?o-i3AT6 SUMMAKY - BAT 7558 01 91 62 454 H S 7 5&1 01 91 '1560 01 yi &3 527 M S 66 490 M S T574 01. 91 67 472 H S 7565 01 91 67 ^92 H S 7539 01 91 69 501 H S 75'. 1 01 91' 7536 01 91 73 77 502 H S 562 M S 1rs>&3 01 91 70 497 M S 7 578 01 91 82 505 M S 7546 07 91 2bUO i>7 406 M S ? 755 V 250 5 tiY!^ HC!;^AL MOxPHriLCGiC STP.UCTUK.6S FXLiri.HTAL &ACi'.iMAI. GLAND NUP.MAL MOR .i:'.1L('Ul(- ^T:<UC"rj''l'^ :iiiA!;-l I <.'.!,; HAL f-;CP.l'H()l.;i'-;lC .TKUCTU-<r;S MKOUl.LA l':r.Li"<NGA7 A N I.) K M A L MO".?nrjLOGl 1; ST.vUCI Ur IS SCL.'.TIC I-iCKVC: NO.'MAL .KJKPHllL llGIC liT i'.UC runf;-; SK,[;j N11;'.;-1AL Mi.);<r'ill'L()GlC S TKUC TUKC-S SK If: 1;,<^.^H. Ml S:; i'K, M...',v; A a Y C.L&riU fKlKK.'.L l-'.Lll^rlUt. (1G i'C ST iU;C'!U'!';S ;';,.';;l.t;l.Y I.LAIj!; l;RGAM' MISS 1N1; "J.NL "A^.ICH M,'r,.'-lfll. M^.'PlinLUG 1C CTU'^^S STRU St'Li:*< ;jnr;-i.\L Mr;i(PH(lL!..l; 1C STKUCrur.E r LV'-i|>!< N.'OE iW'-'.'lL )-10.-'.P!l:;l ;)C 1C SI^UC LYIlril ;\'Ob i.),\;,A:'; USSINL; SKri.i-'-AL .^.USCl.fc H:);'.'-1AL M.'J'-'Ps-lUI.'.'lt; IC Sl^.llCiU-'.i'-S TKArni:'A (;:):<;;AL r'.i'l i-'iiULi;G] C ? n<.k;C TUK c r l.'-:.')C, r.UkMftL lUKPhl.-iL.iGIC STRUCTUHES LU';G '.NT ; :'.ST] T 1AL PNPU^UNIA Ht'.'.P.T t':;"':'.,^^'^ lO!' PI-H.;!.f>;IC STKUCTUKKS A'-.-'.TA MJi'.^AL ;-lUt<Pll'JL.'l;>IC STnUCTU.'^S SALIVARY ^.LANI) NO!U'',\L MORPHULl3GlC rKUCTUKliS LIVi;^ KL;.\;1AL t'aP.PHl;L()UIC STRUCTUSi.S L IV^K C;-<AN'JL''i'-lA LIVES Ll.fJUOY SnF.LUNC LIVER HYALINE UKOPL6T' URGcNEllA 1"I ON 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 U 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 U 0 0 0 0 0 0 0 U 0 0 0 0 0 0 0 0 0 0 0 X 0 X X X X X X X X X X 0 0 0 U 0 0 J 0 0 U 0 0 0 0 0 0 0 0 0 0 \) 0 0 0 0 0 0 0 U 0 U 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 U 0 0 0 0 0 0 0 0 0 0 0 + 0 0 0 0 0 0 0 0 U 0 0 0 0 0 0 0 0 0 0 0 0 . 0 0 0 0 0 0 0 0 0 0 0 0 0 0 U 0 0 0 Q 0 0 LIVER CYTOPLASMIC LIPID OHOPLi:T ALT TABLE XII (Continued) HASKELL f. js. COMPOUND ANIMAL ft! L1VEK HYP^RIKOPHY PAr.'CP.FAS NORMAL MORPH nuic.ic sTi;ur,Tunr-s ESOPHAGUS. MUKMAL MORPHl.lLDi; I C STRUCT O'P.KS STi^ACH NDHMAl. MU;<PHOLUttIC SrKUCTlW ES l-)Ulln^:,^,^s1 f,ill'.MAL MORPHOLOGIC STRUCTU 1;ES C.'l.DD ^.I'-IAI. KOHPIlUL()l,ir. STRUCTURES CrCUl-'. Nn";1Al. M",<PH()1.1)G1C STKUCTUHFS K1DI\"Y .'i'.i'UIAL MdKPIini IIGIC STUDCrUKE s HISTOPATHOLOGY TO-OATE.SUMMARY - RAT f\ 7558 75(>l 7560 7574 7565 7539 7541 7536 7568 7578 7546 75 + 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 6 0 0 '0 0 0 0 0 0 0 0 0 0 0 U 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 U;'r<Ai(Y l;l-'iU!)E'. .'i'lKHftL MllKPHDLDGIC SIUCTUHfS Pr.'l;>TA'Il- AM!' .St'MlMAt V^.'ilCL^. NOKMAl ^l)",PHi)l.l)', It: ?TFUCTU".FS Trsris M:!'f-'Ai. MUl'-pHOL^tiir. STHDC(UUE s fcPl"iI!)YMl ; MII'.MAl. Mfll',PH'lLn(,IC S1P.UC T U c. ^ S IJILI-'DA, MIIP.I-'.AL Munpnu.(i(;ic sypu CTll'T'', hAll'!t'lAN TUBE NUHMAL Ulil'PHIJL I.'G 1 C S IPUC7UI'.PS I'VA|;Y Niii- -IM. MnupmjLr'i^ ic SIKI)CTUKF:S "IIUITAHY G1.AN11 '<rjt'HAL MDl'.PHDl Ul; I C '<T[!1;C 1 U:<.1;L; AD'-'-NAL l;LA'J!) NGI'MAl. MUKPIinLm.lC ST i-;UCTl;:<r S TliYh;l]ll) O.ANI) NUKMAL r^l)KPH()LI)G I C ST ['.L'-.U-'MC-S Tl'Y^I'li; GLAND HKGAN MISSING P'.h:A'llYrtl'I I) GI.ANi') HI'HMAL ^ORPIKIL OGl STI'.U'.TUi.;! S PA'tATHYR.'I I:' GIANU (J^GAH H1SSI', TllY!-'U'j NrK.'-'.AL MllKPHUl I'GIC SrRUCTUKE S 000000 000000 00 00 00 00 00 0 000060006000000000000000 000 00000000.000000Q 000000o 00 XXXXXX XXXXXXX XX 00 XXX 0-OOOOOOOOOO HASKELL .1.' _ COMPOUND ANIMAL It G^r.'UP 9 TH.liATH.RST DAYS pnaT-TK^ArMi-'-ir DAYS 1)1!': r/DIJSE": VALUE INITIAL rieiGHT LI.IH wLiynr FI^AL WEIGHF sex oni.i/SAt.r' ir-'ioiii) TABLE XI I-A HISTOPATHOLOGV TO-DATE SUMMARY - RAT y.^-:-. . 7 -.7 3 75e<, 75'.3 i'(.ii^ 76>.;.a 7&18 ;r&2i. 762<> 7507 75^5 7 07 07 07 07 02 02 02 C2 02 OZ 02 91 91 91 91 92 92 92 92 92 92 92 2500 70 ^- 2i>00 7'-. 2500 79 49 62 63 70 72 72 72 633 M S 519 '1 S 523 '1 S 512 11 j 243 F S 268 F S 270 F S 333 F S 273 V- S 275 F S 291 F S PY!- NORMAL H3rtP,-lU1.0GlC STRUCTURES EXOP-iUTAL LAC^IKAL &LAM:) NUKMAL KUK PIIIJLCGIC ')II'UCIU;-:F.S Ili'.AIi', i';;J ;:'!.', L MU^PliriLll,',! C, STRUCTURES .'ll:Dl)LLA OilLilNGAT A N'JI'.MAL M UP. I'HI:) LUG I C 3T,<L1':TU;;C-S SCI.'.riC Ml:]'.VC IiU:<MAL MO;tPIIULi)GlC ST ?.>-.;'-1U.<^S S;<.1H N'J:'.M,IL HI.),<I-'IIL;LH^IC STRUCTUKES 3;<).M OR I; AC; "1 Sf, !.<& M..',,".;iA^Y GLAMU Mdl-MAL MUKPHOLCU 1 C Sr KIJC lUKfcS \W-':,\A'( !-,[.AMD 11;tl',AN MI5SI ir, liL;;':'-; MAiti<UU NlJRriAL MUKPM'.ILUG 1 C STRU C ru.-'KS S;-'LEI:N NO<MAL MflKPH'lLnGI C STRUCTURE S LY'-iPH Nl)D[- NU'^MAL 'lUKPHUL..).'. 1C STP-UC TL'.l^S L'I'.'I;"-"-' N03L 'IKGAM t-iIS'.lr')^ SK-'-LETAL i-lU.SCLc M].;''<MA1. I'-iaKI'HnLliy 1C gO. ST<UC7Lli'.F.S _ Tr'.ACHfcA NUKHAL MI'IKPH'.UOG I C STKUCTUR ES <0 HJ'io NU^MAL HO'^i-'l-lOLUGI C STKUCTU-IES 3 H.INI; If-iT'-'^STn 1AL PtieUKHMlA C^HLAI^T NPFMAL M..)KPHiJ'>.0..;IC STRUCTURES oAOIHA plui'.NAL MUid'ilULUo I C STRUCTURES 0 ^SftLIVARY GLAND NORMAL MnRPHLlLOGIC S STSUCTURES _^IVER (ttIVSR SIVSR SIVER NORMAL MOKPHOLOGIC STKJCTURES GRAriULOl-iA CLOUOY SDELLING HYALINE QKUPLET DEGENERATION U 0 0 0 0 0 U 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 U 0 U 0 0 0 0 0 U 0 0 0 0 0 0 0 0 0 0 U 0 U 0 0 0 0 0 0 0 0 0 J U 0 0 0 6 0 0 U 0 0 U 0 0 0 U 0 U U X X X X 0 0 0 0 0 0 0 0 0 U :> ' 0 0 0 0 0 U 0 0 0 0 Q 0 0 0 0 0 0 0 0 U 0 X 0 0 0 0 0 0 0 0 0 U f) 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 U Q 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 a 0 0 0 0 0 0 0 + + + LIVER CYTOPLASMIC LiPID DROPLET ALT ti a; a i-i 4J ri 0 u <;' 1-1 1-1 X S M < J" 1 N Ift i\t r. f 0> 1 ^, 0- --< 01 ' :- 0 r^ < -- a. > N 3-) 0' in -> r- < n o r- 1*1 fO o r-- 0 0 C c; o c3 0 -) 0 0 0 -3 0 J 0 " 0 0 0 S0 3 3 0 0 0 0 30 3 0 0 0 0 0 30 c3 o o 0 0 0 C 0 0 x 2 0 "^ 3 0 K x 0 0 0 0 0 x x 3 0 0 C 3 x x 2 0 0 => 0 x 3 in 0 0 0 30 0 0 3 (r m h. 0 0 0 0 0 x X f- 0 0 0 0 0 0 0 ao in r- 0 0 0 0 0 0 x 0 0 0 0 0 0 0 0 N <o E-i 0 0 0 0 0 x x -4 0 0 0 0 '0 0 C 3 0 (M O ? > 0: <l CO 0 0 0 00 0 3 0 a: -1 r o h- 1/1 0 0 0 0 0 0 x 0 0 0 0 0 x x UJ m 0 0 0 00 0 >- N <. o 0. ^- 0 f- 0 0 0 0 0 C3 -< > o 00 r- -J 0 . co + I.' r < a. a t in r- 'r 3 >n t^ m + r<* in f- m * r in F- c & c z j < s 3: 0 z 0 0 c 3 ^^ 1.) 3 t-- 1/1 U a 0 0 0 0 0 0. 0 C 0 C 3 tt 3 ZS u 0 3 3 a X. 1- t-- 10 1/1 u 1-1 1.1 u> G J 0 0 C3 0 0 =3 0 o c3 0 3 0 uf\ \r\ uu aj 3r a: 3 3 (-- t-- (J -> 3 r? ' -n ^~- t-- y i i/i t ' i-> : S C3 = <-> -j -% 0- ^5 a J C=3: 0a:. c c < Z ri s >- VI 3 s 2: UJ J t. <; l y < 0 5 S < v ^ c: I-- cr -J < X ^-- u 0 s^ --i --i x r? z c' r- < i^ c: .Z ^- I/? >- t/1 -^i 3 r -1 .J "JJ o G 3 ^ z .3'. 0: e-j LJ L'; <^ yi T; Q z : c 3 > < -1 !^ {-'. !-! --; ^1 J 0 v, u; f~ UQ ^ a-' "- 3 yi uu c; ^ u U C 3 0 0 0 0 0 0 0 0 C 3 0 0 0 0 0 0 x x 3 0 0 0 0 0 0 x x 0 0 3 3 0 3 0 x x 0 0 0 0 0 3 0 0 x 0 0 C = 0 0 c 3 3 x x UJ a: 3 t-- U 3 >- t/1 -3 > 0 5. ! 5 2 ^J ,"; .3 W ) 'u -J < ~ r 3 :;> 3' >-- -i (^ z 3 CJ SL. X '-^' t-- 'J 0. ! 1/1 -; U 0 -- S V -3 tl- i/11^ > -i C < -r -J = 3; < 1- c- LC "^ T: a: a ci: j- >- s 11,' '-^ yi 5 -J ou < ^ 1 IT, '<. -3 ^ 0 ^31^ =. i-i -J ; >- 3 1- Cj K - < r -' ^ '-:' t- 3 .; .r j ;; r 3 i/l t/rt t- UJ ^3c ^^-- 0 Li--- l-> ;/) 1/1 0 Q; =) t- (/n -- ^5 '-i -> J ) h- i/0 L. ' ^3 >- J ^J .3 ::3 C' ^1 0 U Z 3 -- c: ( ^ *- -- (- i ;-i: I -J ^ S. 1/11- ';:3 ': - Q. -3 =1 1.3 3 l/l l/l ") J - Q. L/"1 G: :: r E --J ^ =- a s -s LJ L-^ ?: s; / u' i r a. ^'- "^ yi < -2 3- --L ^J -1 < T -1 s: -- S < 2 T. 2: (S -- -j E < -1 ?: 3^ '3 q <-j 13 >-': Z Ci: 0 0 = 3L 3: < < 3Z E 5 o z z <; 0 3 5 < Qa ;2 C <t E 0 ? L- S z < O '-J 7. 3 -J J h- < J) < l/l ?! 1/1 -J - .^ -~ -' z ^ >- L'! ^ CL ~5 LQ ^ .: >- ^ -l 0 LU =.-'- C:: l 0 ^' 3 l - t-- ^-- ~ -J 3 ^ --- -- i J ^ 0 0 -) -) Z VI <^ < <t Cj u 0 S J --' -< -^ -- ^ -3 J) -3 3 ^ -) u-l Q; t-- a- 2 C 1-1 > ">->-) ^ I L': '-- -X < 3 X. i/l 1- 3 ^-- < 2: i- >-&-) '.; U- 3 -1 -; < >- 1:'. ^- -J >- ^-; >- C.' l/l ^; > CL >- < :.: ^ u-- L') (-; u- 1- C" a. 1^ < 3: 1- :- 0^ r- i, ompaity Saniiized. Does noi contain TSCA CB! K\ |y i- ) 3 sos' 1M M <0 in 0 0 0 3 .n N| t ^ o a a CTn h- rt PI .0 0 ^ ."> ^- 3 0 0 Fm 3 1 0 1-- /! N Z .n M .f Z i^ .0 n - 2: 1^ 0 in f>n0 a 0> O n ,- 0 0 o r- n r z 1/1 ^ in (y 11^ '0 l .0 o> in f- r- m .0 TOO' in f- 0 CO 0 >0 ir> O CO 0 .0 /> -< a: ifl (M m r s M ^ -* N M ,0 r- o nift .r 0 33 3 .0 .""1 m s >/ in .0 ms: f- f N m >o o m n >r o o> ^0 .> in m E-l i-r co (M 0 0 0 tl. 1^1 -< 0 0- 0 CO 1ft l in r^ <M >' v < o -a <M 0 f~ *-* U. t/l o o o> 0 t^ 0' N o in 3 h- ril l/l PCI Ul t~ 1-1 < i-i 0 X 0 la h- ^t-) > PQ l 0 l 0 fc4 C' 01 N a o (? in F- 3 COM 3' 0 0' l"> f- rr 03 (N '-'00' .1 r- o o 0 1-m M o in o rifi (^ 3 ^ 0 ^ l."> "^ U. 1^ r- <M CTl U- Ul ^ m CO U. V o N a. 0 >- :2 ^ 3 C- n (a N 3 0 0.a1^> . o n iOft f ru o a. /> 0 (M .-.'i en R; 3 0 C> -'i r~ 0 r^ 0 1>- IA M o u. 1/1 ^ IN .-i 0 r) I 0 t? -3 f- 0 r- 0 0 Tt <M w u. i 0 m ^^^j V) > < a u-' 3 '-^ I- 3 t- UJ 5< - Z -I I C K > -- ;- ? >- ^ L? -- -< h- L<.) i-/j S 3 ^j o =^ :u a: 0 -J -- 3 < 0 < U- t/1 < 0. ^~ --1 --> < c S 3 < -- ^ U '-Q U-- i-- ^ ^ x *. 3 -- 31 -. <t 0 1- a. a " J L- l/ C; 0 -1 Z -1 3 uj a f a. l/l a: < a r u 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 x 0 0 0 0 0 0 0 0 0 t0 00 0 0 0 u 0 00 00 +00 0 0 0 0 0 0 0 0 0 0 0 0 0 0 00 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 C 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 00 0 0 .. 0 0 0 ^ 0 0 0 00 0 0 3 0 0 0 0 -)' 0 3 0 ^ 0 0 0 0 0 0 oo 0 0 0 0 0 0 0 0 0 0 0 0 o 0 3 0) 1-) n: i/l m V) tU/-)' Z ^-1 0: V. t~ C UJ <-') ^ t-- 1/1 1/1 a: 3 3 0 3 (.1,'); Uy J UaJ: 1-> C; 0 < ^ 1/1 T " 0 01 h- a^ 0 U 33 !- f- 5 UJ 3 -J U 0 '/l ^~ U i/l 0 3 3 r- >- U 0 0 0 t- U < IU l- K -1 3 < >- 0 U U 0 l^; 3 --i 0 U a: ) 33 -1.3 h- S 3 a. (~ l-> 3 J S <J f^ -^ 3 C rj: 0 "X UT Q. Z 0 LJ 0: >- cr -J XI- -t 3 ^ k- 0 3- 3 C; 1/1 0 0- L; 0h;- 4'-<(1/-) >1/-1 0- 1/1 UJ 0 ? Z irt 3- I Z J ^ 1/1 Z a: i3 >-- a. V &- - -; S S l- U 0 U UJ Q -i V" C: z U -J x < ^1 a <^ 2 U E -. U n f/i C- -3 J J 0 --31;'-) U < --'^ ^ S -- t C; 3 ,3 J-. C! 3 -1 0 . J) 1- 01 0. 'Z a a: -i -j - C -) --j s" /^ t/l 5.: .3 Z -y) a < -1 S: 0 -J -J -i -10. 0 '-S -J ^ z < J < ^~ E a. s^ r i-n -j OS J uj a: C < -J CL Z: 3: -> ^1 U 0 3: -i 'l. 0^ aa-: uji o.1: 0 3 < ^ Cl- <l al <^ &. < U'. V. ^ 0^30 1 =) hS < r^ Z 0 ^ ?'. ^ < '^ -J t- ? E 0 Z S i/) l/l < d. 2: Irt C^ 0 < -J Z 3 '1. < >- .r J -j -i d LJ 3 ^ -i 3: < s. -J > < ' '<. 'I C i.^ ^y " - " C -J >- >- -j ;' -;<-) 0 1; l-l I<S: z 7J - -J .-. a: 2 '-3 l> I 1.) < 3 C-: -l 5 '- C < I3.-' -j '-. c -t - <x Sl t/1 z l-J ^J 1-- t-- S I.''! ~. r-. U ^c t- < > 1- ~: XUJ &LI') IIUT CJJ: -- 3' i- 3 >- < ^~ ;? LU a. L1-' S; -J 3 1.<-1 y 2 -- K -* '.J -.11 -J 3 > > > > > -'K --i '-* ;^J c r-, <- -'^ '/i -- i.) 3 -I -' ^' i ^' - .; - < -1 ? -J (\. >- 3 >- ^ 1 -- ^ i/^ 3 ^; '-; ^: :r\ h- u- -J -J ^ 't 1/11- -1 ] J >- L- i) ^ '' r\ '-"' " !/ -' i/' 3 LJ ^ l/l i/. -...-- company Sanitized. Does not contain TSCACBt -- - - - ^ TABLE XII-B (Continued) HASKELL fl COMPOUND ANIMAL LIVER HYPHRTKOPHY PANCHrAS NORMAL HUHPH r.lLUGIC .STRUCTUl'.FS ESOPIIAGU;; M1KMAL MORPHDL ;JGIC STKUCT UI;ES SICMALH M'JK.MAL MnRPHULOGIC STRUCTUK F.'; nULinF.NUM .'niliMAL MHKPHULUGIC S7RUCTU ;'&S C;;LUN r<lj!'.'.lAL HU!<PHllLrjGIC STP.UCTUHES CI:CUM (II.II-'I-'.AL WUHPHOLDOIC STKUCTU^ES KIOMl-'Y NO.'HAL MllHPHnLnGIC STKUC^Rt- sft I'-IAI;.YIH AOm;R Nlll<MAl. M']"P;<1)L(H;IC HUC7 Ul;l. ; B'flSTATP A^'.l SLMIHAL VI-S1CLF Mll'.MAL <MORPil()Ll-'i..lC STPUCTURES CR^TIS Nlll'.MAL MI)imiULf)(;IC STPUC7UKE aPiniDYMIS gui'ES QJTFCU'. 111);-.'',AL MUHPH.'Lf.-GIC S1MIC NDKilAL Ml.lKPHiILOG 11. S r W p6TlKI'S CfALLt'Pl.tN 11)111: M;k,".AL M(!KPHULUUIC S gn<uctui<r", 3l.1Vf,"Y r-,D-'.",A'i. HIKPH'll.nO 1C 'iTKUeTJ'tcS 2)'l TUlTAi-iY 1,L'\H') NLIK^AL MORPH'JHIL.IC OSTHUC1 U'<' ; 3 AI;:.'l'MftL 01 A'-li; NORMAL MOR.rH:)L|iC, I C ST (a'K>jr,Tu",r^ 5'T|]Y"!H t> OLANi) ^HRMAL MilK PHOL UG 1 C ST -41; II!. T UK I.'; t/n !(Y!(('I " CLV-'n ilRGAN MISSING 0 pAi-.ft IIIYI'.I'.I ;. GLAND l-;ll;(MAL MOKPHOLnGI '" C STHD'-.TU:'; ^ 0 en PA^MIIYK'lt') GLAUD UmAN MISSlriG 1HYMUS ;;li",HAL MORPHULUGIC STR.UCTURR HISTOPATHQLOGY TO-DATE SUMMARY - RAT 7&32 7602 7603 7613 7590 7589 7600 7614 7542 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 C 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 (.' 0 0 0 0 0 0 0 0 0 0 U 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 X 0 0 0 0 0 0 U 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 X X X X X, X X X X X X X X . . 0 0 0 0 0 0 0 0 7572 7547 755 1-1ASKELL IS ^ COKPCUNO AM I HAL If GKDUP II TKEATMGNT OAYS Pn^T-T-ilrATMENT DAYS DIRT/UflSF VALUt; INITIAL WEIGHT H)H wr-IC-HT FINAL HEIGHT S^X ul l:!;/SACl',IF ICEiU TABLE XII-C HISTOP&THOLpGY TQ-DATE SUMMARY - RAT 7570 03 96 SOO 96 432 M s 7^33 03 "6 500 96 542 M S 7631. 04 97 7586 04 97 500 500 56 61 245 F S 242 F S 7599 04 97 500 63 259 F S 7625 04 97 ,7627 04 97 500 500 68 70 268 F S 301 F S 7628 04 97 7604 04 97 500 500 72 75 293 F S 281 F S 7609 04 97 500 77 333 F S 7626 04 97 500 78 346 F S 7593 04 97 500 8.2 309 F S EYI -;')KMAL "nr'.fHDLD^lC S T 1;UCT UK FS i:>;'l-'.ol K.L l.ftC"lMM. l,l.Ai^i) H'JxMAL MOK i''|ii!LI!l.i',. '.r;'.U',.UJ-.l-'. Ul'.M;< Ml)("AL :Hllii>lli)l.l';;IC .STRUCTUF.tS HI-l.'iJLLA ("(L'^iGAT A NiW-IAL l-UJIU'HOt IIG I C ST!!liCT',);.'.i. s SCIMir. ]>/,ivr ^OKMAL MOP.PHi)l.')';I C ST PliCTi,)-' ( S .SKI''-! ?11.";".AL ".'l^CHDI.llljlC '.>TRI)CTU!<r3 ')",1 !1 ;ii'(;A'; M 1 ",'- 1 I', '1AM!'A'iY ;.l AND '; I ]." ,< A L ^0:< l'ill.( !!> I C St KUCIll.- i.:> MA^;;IA,-.Y ;;1 A'lf) (1U.GAM M 1 S S 1 N0 CIVT'ftii?. '> ;'>-',AI.'-'l',< NI.II'.MAI. .rtJUfHill <)G If. STRU Si-'l;!;) f; f;(i.<MAL Mlji.lPHi.;! ri,1(. STKUCTURE <, ^ LY.'-'H r;iTir rnHMAL ^iii'<riii!i.i;r,ic STI'UC T uw', I.Y.WH ^<)!1' il|'C;A^^ MI'.SINt. '.KS.'.IAl. 'ID'.r.LI, MUKMAL HI l.'.l'Hl'L H; I C STanr u)i:''s 1i-.,S.l.',<l NilKi-AL <1)R1'HHLI)G1L STP.UCTUM rsp- LU'C> ^()''M,lL M i;.l; pm:L''IU 1C STKUCTUi<r-S 11)1%; i'll i 'ST i 1 IAL PN!:l)H',.)NtA nr-.WT ^;r""-lAI. :11ii<PH(;Li)l^iC STRUCTUKl;S AOfl A i'; 0 ;.;. A L ,"ni;PH.lLHi;IC STRUCTUKi-S SAlgVAKY l,l;AM) t.OKHAl. MORr'HDLtJG I C S Tr.Ltf riJiU.s Ll5'K ('i'n'.^.AL 1-1CI!(PH;!LOGIC L '>\5'R U1',ANUL;:MA STRUCTUKF.S LIV-lR LI^R > (.LOUDY SWtLLI^G HYAL1.MI- DK'.'Pl. r; T UE&SNERAT I ON LI\QBK CYruPLASMIC LIP.'IO DRUPLET ALT a o u . x M 1-1 M ^ X >- 0 00 1/1 UJ 0. I.) 3 h- UJ 01 t 0:' 3 -- 0 3 55 l- C 3: l-> 3 ^ E 3 >- 1- i-l ) ;/! ? - U LJ 3 l/l 3 3 a >/1 a: v. h- ^ \- u - j^ 0 i/i;/) 0 0 - ^ ^j .Z -1 u '-> I-' 3 ^ 0 -3 0 ^ 3 3 C i c: CL l J ^-> l cc. a = r <r s z >- on 2C 0 : S a. -j -- r -J -1 =.; a: : 0- =: < < c 3 S J .J <; i: 2 3; ^ -) 5 <1 ^ i ; : 3 z K < < 3: LLJ C X S '' CL >- in >- LQ /I ^c <r u ^ 3 3 ^; 2 Z i- 5 S u $ 0; 11- i-> ;? i/> s C 3 > =: -'^ -J L-' --*<; J ^ CC ;: c. r L; 3 -r. i-; 3- U U > L.-0 ^ 1^ -1 i" 1,J < EC S 3 3 ^ - -i 2 >-) J 2 3 -l Q: ^ 1-- --J /i a U C -- I.) UJ l/* 'J3 J > ; 0 -133 < -/ -J 7 < -- -S ^ 3 2 a: E x. C ^J GQ I/' 3 ) ^ ^ -3 <; J i^ < 0 ^ J) s c C; 1*-' --1 ^ >- 3 f t-- < Lj S '^i 3 /I 0^ ^ ^, p- )- e" E U) 3 =- -- >/: u 3 -C. t-- i/) -) 0 -J a. at: C l < E Z I/' E >C 0 1^ C- ^ i.Li h-- =) i/i U 1 h- 3L JJ LJ ^ t-- i-) ^ ' ^ ^ > ^ ) >-) U ^ ) ^J '^ s ; J ) s /I '"' : S a:' S t. > -- i a: -j -J ' < ^ < -- I 7:: - i-' a *^ "j ^ < 32: -1 i-/) i^> 1/1 =- 3 ^ UJ - t-- >- - -- 3 -^ Li < ^ L; -,' 0 - ^ U ;'- t~ -: CL Q , ^ - UJ ly t.9 3: C 3 3 23 U J 1- /-3 0 >-) :s : 0 -J 0 ; c: J) C ) (fl i^ l/ L^ a C ^ J < ? a ^ < 3 -- Q- >- J. X-:-> S U V, - J .n i[ "-- ^ S 3; i z -a j < 3 '~3 -1 ^ 0 Z 3 < 0 C1 c r Q. Q Q: ' 5 < Z C ct Z Q ri1 -J u; i z 1/1 < < c JJ Q S .'- -- Q: 300 J) ,-; 0 ? <>--; >)--. Z 3 -C t/) l-i: t- 3 3: <t < E >- -/. n = -( *-- Q. 0 .--: >< 3^ ^i- - (. Company Sanitized. Does not contain TSCA CB!1 s>- ilASKELL # COMPOUND ANIMAL 1 GROUP TREATMENT DAYS posT-rri-ATHENT DAYS OlCT/mS^ VALUE INITIAI WEIGHT L'JM HEIGHT (: IHAL ni. 1GHT sr:x nit D/SACRir-ICI-D TABLE XI I-D HISTDPATHOLOGY TD-DATE SUMMARY - KAT 7552 7579 7567 7554 7562 7556 . 7583 7630 761.5 7620 7622 76 05 . 05 05 05 05 05 06 06 116 06 06 97 97 97 97 97 97 98 98 98 98 98 750 750 750 750 750 750 750 750 750 750 750 7 68 68 70 73 76 78 60 62 63 63 67 4B2 4as 640 492 530 575 345 304 303 323 291 2 M M M M M M F F F F F S S S S S S S S S S S frY'-: N()ri,"iM. MUi'.Ptini.DGIC ST I'.UCTIJl! FS I X'.'XIU TAL LM.KIMAL (ilANI) NllP.MAL MUK I'fiOuiGic ;,T:'ucrui'.i's li<Al:.; ',()1'.",\L Hl)l-.PH(ILI''i.;lC ST1WCTUHFS MEDULLA OtIl.i'MGArA NORMAL rtORPHtJLOGI C STI'.ULTUittLS SCIATIC NEHVI- f;nt'MAt. MDHPI-inLLK; 1 C ST K'JCTUHfiS SKIN NC'R'-'AL fl"l!<PHIJLU', I C SrRUCTUKtrS SI'vIN IJ'itiAN MISSING K/>M:'".Ar;V GLAf-IU NDP.MAL MDi'Pi-IULllG 1 C ST "UC'HJUb'S HA;1",AHY GLAND OIUiAN MISSING niI'Ml; i1Ai<K['i'rl fiClHKAL .'llJHP.'lflLClG 1 C STRU C I U" i: S SPLEEM NIJF.MAL HO,'<PI IOLUR I 0 SlkUCTUi',C S L'/M;'H Mjl;L NORMAL MURPHDL UG 1 C :;ri'.UC TUK&S LY'-'I'H ':'.;01; Hki.iAN M1.SSIHG SI'.El.trAL MU:;CLL- NUKMAL MUKP11ULDG I C Slh'.UCnil;! S Ti'.;>Cili''A !-il'l'..''lrtl. M()I.'.PII;)LI.'.;II.; S11(UCTUr< ES H;.\G NU:<MAL MORPHnLnGIC ST!<UCTLIi<ES L'J'IC, mi ^i-STIT I.IL P^UMllNIA lii-'AP.r i\>!itl-'AL l''i)KPH'.)LUGIC 5T SUCTURirS. AC.-t.TA ^lno.:..AL MORPH'-;LU^IC STr-.UC TUrltS SALIVARY GLAND NOKMAL MOKPHOLOGIC S TP.UCTURES L HVFR Ni.JP.HAL MORPHOLOGIC STRUCTURES L1VC-R G?,AMULUHA LI';?!? CLL'l.lOY S'riRLL ING L!VR HYALINE ORUPLET DRGENER.AT1 ON LIVSK CYTDPLASMIC LIPID DROPLET ALT 00 0 0 0 0 0 0 0 0 0 v.^. TABLE XII-D (Continued) 1ASKELL /( COi-'PULIfO ANIMAL a LIVE.-P. HYPtHTRllPHY "ANCKEA3 NORMAL MOHPH '-1LOGIC Sn'.UCW^IrS ^S'lPIIAGUS NORMAL MUKPHULUL.1C UKI'S STKUCT STl,'MACH NOK'.iAL HOKPMIJLOG I C STRUCTUR ES OlDDENUM NiKHAL MOKt^l-iULllGIC ^TRUCTU Kl;S Cni.UM ^fiRMAL MORPHUL'JGIC STRUCTUURS CECUH MURMAL MfJSPHIlLt'hIC ^TKUCTURFS 7552 KlO.'.irY NUI'.MAL MI)l;l'H''iL OG I L STKUCTUKfc S it UKINA;(Y aLADDEit NUKilAL MOPPHULOl, STUUC runr-s PP.O^TAIC Al'.'u SF.M1NAL VESlf.LI: ND.<MAL HOP.PUf'LOGI (. S TKULTUH^S TEST1S NORMAL MOKPHOLOl; 1C STRUCTUrtF S KPlDimtllS NU:;HAL MUKPHOLllGIC STi<UC TUR;;S UTr!<US. NOKrIAL MnKPHULllGIC STKL1 CTUIIES FALLIiPIAN rui'i HC1RMM. KIJ'-tPHJLLlu 11 S T;\UCTURRS ;jyA;',Y FORMAL UnRPHULl-UK: STRUCTUK6S l'IH);TAHY GLAN.) NOKI-'AL f-iDRPHCLHG I C S1 KL'Cr LIKES AL;'r-;;t;/,L GLANU Ni.ll'.HAL MOH.PHllLD'J 1 L ST UCTU,i(:S THYHOli; ULANO NORMAL MC.I'.I t'ifllLnG 1 C ST RUCTUKI S TI-Tl'ROID GLAND LIKGAN MISSING PARATHYRCIIO GLAND NORMAL MURPHOLOGI C STRUCrii.lES PARAIHYROID GLAND OFSGAN MISSING THVHUS NORMAL MOrPHOLnG'C SrRUCTURE S HISTOPATHOLOGY TO-DATE SUMMARY - IR RAT 7579 7567 755^ 7562 755& 7530 7630 7615 . 7620 76^2 761 ^ TABLE XIII BODY WEIGHTS OF INDIVIDUAL MALE DOGS FED Group I (Control) : Dog No. 1150 1154 1158 1162 0 8.1 11.2 11.4 9.5 1 8.2 10.9 11.4 9.2 2 8.6 11.0 11.4 9.2 .<3 8.1 11.1 11.6 9.4 4 7.9 11.0 11.1 9.3 Weeklyj Weiglit in I Cilogr.uns 5 6 7 8 9 8.0 11.0 11.5 9.5 8.1 10.8 11.5 9.6 8.2 11.2 11.8 9.7 8.2 11.1 11.8 9.5 8.3 11.3 12.0 9.6 10 8.2 11.4 11.8 9.7 11 8.4 11.2 11.7 9.6 1153 II 1155 B(^5^0^0D^^Q^n^^^^) 1159 1165 1152 1 O fin ,0, (750 ppm o 1 03 IV 1156 | 1160 1164 1151 1157 If---------- JJ 1. (2,500 Ppm . s ^^^^^^^^^^ 0, 1161 1163 0 0 (0 u> g ,Sr " 0 s. B>, 5 -0i1 0 ? 0 10.8 9.3 14.0 9.0 11.2 8.3 9.1 7.8 10.8 10.5 9.0 9.8 11.3 9.2 14.9 9.5 11.0 8.2 9.1 7.5 10.6 10.8 8.7 10.0 ' 11.1 9.4 14.0 9.5 11.1 8.3 9.0 7.6 10.6 10.7 8.8 10.1 11.4 9.5 14.3 9.8 11.3 8.4 9.1 8.0 10.7 11.0 8.8 10.2 11.1 9.4 14.2 9.5 11.4 8.1 9.1 7.8 10.4 10.8 8.8 10.0 11.2 9.4 14.2 9.6 11.2 8.1 9.3 7.9 10.8 10.6 8.8 10.2 11.3 9.5 14.3 9.4 11.3 8.2 9.3 7.8 10.8 10.8 8.5 10.3 11.5 9.6 14.6 9.8 11.7 8.3 9.5 8.2 10.8 10.9 8.9 10.5 11.4 9.8 14.4 9.6 11.3 8.4 9.4 8.1 10.8 10.8 8.8 10.5 11.6 9.8 14.8 9.8 11.4 8.2 9.4 8.0 11.0 11.2 8.9 10.4 11.2 9.8 14.8 9.9 11.7 8.6 9.4 8.0 11.0 11.1 8.8 10.6 11.5 10.0 14.9 9.8 11.5 8.3 9.4 8.0 10.9 11.0 8.8 10.8 \a.Sy' TABLE XIV BODY WEIGHTS OF INDIVIDUAL FEMALE DOGS FE: Group I (Control) Dog No. 1137 1141 1145 1147 0 10.5 11.8 8.0 8.9 1 10.6 12.0 7.8 8.7 2 10.7 12.0 8.0 8.6 3 10.9 12.0 8.3 8.8 Weeklyr Weigl-it in I Cilogr;ims 4 5 6 7 8 9 10.7 12.2 8.2 8.6 10.8 12.0 8.2 9.0 10.9 11.7 8.4 9.1 11.0 11.6 8.5 9.2 11.0 11.5 8.5 9.2 10.8 11.5 8.4 9.4 10 10.9 11.4 8.8 9.3 11 11.0 11.5 9.0 9.4 II 1135 8.2 8.1 8.1 8.3 8.4 8.5 8.7 8.5 8.5 8.5 8.2 8.3 1140 7.0 7.0 7.0 7.2 7.0 7.1 7.2 7.3 7.3 7.2 7.3 7.3 (500 ppm , ,,, 1143 8.1 8.8 8.0 8.2 8.0 8.4 8.8 8.6 8.6 8.4 8.5 8.6 r 1149 6.3 6.5 6.6 6.8 6.8 6.8 6.8 6.9 6.9 7.0 7.1 7.0 J i ^ ia.s s (0 Q. a 0 <o w 3 0 -r* CT 0 3 ffi. 3 - 1 0 > <"> Of 1136 13.0 13.3 0---------------- III 1139 8.0 7.8 (750 ppm . 1144 9.2 8.5 1148 9.6 9.8 1134 iv 1138 (2,500 ppm . 1142 ;r^--^^^--^^^--^^*1146 6.0 6.1 8.8 8.4 9.6 . 9.2 7.0 7.0 13.2 7.8 8.3 9.4 6.1 8.9 9.0 7.1 13.3 7.9 8.8 9.6 6.2 9.4 9.2 7.0 13.4 7.9 8.6 9.4 6.1 9.3 8.8 6.9 13.7 8.0 8.8 9.8 6.2 9.5 8.4 6.8 13.8 8.0 8.7 9.8 6.4 10.0 8.4 6.9 14.1 8.4 8.9 10.0 6.4 9.8 8.4 6.8 14.4 8.5 8.8 10.0 6.5 10.2 8.5 7.2 14.9 8.4 8.8 10.2 6.6 9.8 8.6 7.0 15.2 8.4 8.8 10.2 6.6 9.8 8.5 6.8 15.3 8.7 8.9 10.2 6.7 9.5 8.4 6.6 TABLE XV DIET CONSUMPTION OF INDIVIDUAL MALE DOGS FEP Group I (Control) Dog No. 1 2 1150 290 350 1154 328 365 1158 410 400 1162 318 316 II (500 ppm III (750 ppm 1153 345 389 1155 326 403 [ 1159 429 555 1165 330 . 352 1152 320 441 1156 241 279 1160 314 356 ^ 1 1164 308 333 ; 1151 320 IV 1157 379 i I C------ J:2,500 ppm 1 1161 1163 260 296 i.-5 N" <0 fla a 0 -.3 VI -S a. 0 1 3 -4 % 0 > 0 Si 416 417 306 333 , Av erage Daily Diet Consump tion i n Gram s Duri ng Week 3 4 5 6 7 8 9 10 11 12 289 295 313 336 300 361 317 291 360 346 340 363 310 326 358 350 325 374 324 391 390 370 432 381 415 393 384 417 375 394 361 336 345 335 336 351 258 318 321 339 380 427 439 384 415 381 408 373 419 383 339 392 417 387 414 404 348 421 394 416 500 489 541 517 567 502 525 544 569 461 339 346 338 320 386 327 381 361 375 352 435 429 329 382 414 429 390 381 333 412 269 254 267 263 270 301 256 322 343 356 375 381 349 326 327 359 293 322 389 352 306 350 286 259 315 328 262 293 281 221 396 421 438 370 409 429 430 380 448 371 418 404 432 387 426 426 361 382 420 382 370 347 305 314 371 384 314 304 355 355 352 318 370 341 330 356 293 314 323 300 ' TABLE XVI DIET CONSUMPTION OF INDIVIDUAL FEMALE DOGS FED Group I (Control) Dog No. 1137 1141 1145 1147 1135 1140 1143 1149 1136 B--B| III 1139 (750ppn- 1144 1148 Iv ^ j^------& 1134 1138 1142 1146 1 382 428 263 200 251 265 258 349 427 304 197 346 279 300 275 244 2 396 373 287 198 323 267 297 362 409 317 290 372 270 448 277 267 A\rerage Daily Diet Consuniption in Graims Duiing We'ek 3 4 5 6 7 8 9 10 11 12 406 364 338 378 386 391 312 316 338 358 372 453 287 286 280 395 356 401 470 452 303 284 281 331 290 259 226 311 312 285 280 321 334 262 327 321 359 295 318 306 329 332 320 329 319 35.6 355 305 244 202 283 277 254 273 329 301 232 231 200 261 375 380 392 386 343 338 362 326 247 349 369 362 412 359 369 366 373 337 367 381 368 426 388 429 450 492 538 449 455 403 317 357 309 283 '319 331 293 257 332 310 304 305 298 313 291 271 259 296 308 317 361 364 382 353 335 318 360 244 272 402 280 284 320 293 311 261 253 251 276 260 422 414 350 399 377 370 398 352 233 254 309 277 183 261 344 328 342 256 352 421 261 252 284 256 255 282 217 198 276 104 Group I Control Dog No. 1150 1154 1158 1162 II 500 ppm 1153 1155 | 1159 1165 III 750 ppm 1152 1156 1160 [ 1164 1151 IV 1157 2.500 ppm 1161 1 1163 TABLE XVII CT AVERAGE DOSES OF INDIVIDUAL MALE DOGS FED] J Avera;ge Dai ly Do;se in :Millig rams /K:Llogr<ams d uring Week 1 2 3 4 5 6 7 8 9 10 11 12 ,, ,, ^, - - - - ,, ,, ,, - - - - - - - - - - - - - - - - - - - - 45 50 17 51 62 18 42 55 18 51 53 18 62 86 29 63 73 24 74 85 31 87 94 29 192 252 94 230 253 97 190 223 105 192 214 86 19 . 20 21 22 17 19 18 18 28 22 23 25 31 28 33 28 99 103 93 101 99 87 79 92 17 18 17 21 22 21 18 20 17 17 20 17 26 27 28 24 25 27 26 26 29 25 30 30 86 95 99 90 98 99 91 106 109 84 79 85 18 16 18 16 18 22 20 21 18 18 19 16 20 18 19 18 26 25 22 27 23 29 31 32 23 26 31 28 25 28 26 21 99 86 102 86 82 85 96 87 89 86 101 100 70 75 75 71 Group I Control Dog No. 1137 1141 1145 1147 II ^5--00 --ppm D 1135 1140 1143 1149 1136 ^ ^in 750 ppm 1139 [ 1144 1148 1134 IV 1138 2,500 ppm 1142 | 1146 TABLE XVIII T T ' AVERAGE DOSES BY INDIVIDUAL FEMALE DOGS FED| S Ave rage D.aily D ose in Milli grams/I.Cilogi"am during Week 1 2 3 4 5 6 7 8 9 10 11 12 ,, .. . - - - - - - - - - - - - - - - - - - - - - - - - - - - - 44 57 20 20 19 19 19 21 54 54 20 20 18 19 23 21 44 50 23 24 24 22 20 20 78 78 28 27 30 26 27 26 69 66 21 24 21 23 24 26 82 87 30 34 29 26 29 30 50 74 26 26 26 27 25 23 76 83 28 29 30 27 25 24 300 284 115 114 129 116 121 102 224 334 115 110 93 108 95 92 188 195 85 77 53 78 102 98 224 245 93 90 104 94 94 101 21 18 15 12 16 16 14 18 21 19 14 20 27 24 26 27 28 22 22 20 26 23 29 27 22 25 26 26 27 18 20 29 96 95 105 97 99 90 61 67 99 104 105 125 76 72 103 40 TABLE XIX SUMMARY OF HEMATOLOGIC MEASUREMENTS ON DOGS FED ppm in Diet 0 Erythroeytes 500 x lO6/TM!3 750 2,500 0 Hemoglobin 500 gm/100 ml 750 2,500 0 Hema.tocrit 500 % 750 2,500 )h 0 Leucocytes 500 x 103/mm3 750 2,500 0 Segmented Neutrophils 500 750 2,500 0 Non-segmented Neutrophils 500 % 750 2,500 0 Metamyelocytes 500 750 2,500 Pre-test 6.62 6.05 6.10 6.24 16.7 15.6 15.9 16.0 44 41 42 42 11.8 12.7 11.1 11.4 66 64 65 68 0.8 0 0.4 0.5 0 0 0 0 MALES ' Months on Test 1 2 3 7.51 6.87 6.55 6.59 18.0 16.7 16.3 15.7 49 46 44 42 7.33 6.70 6.65 6.36 18.0 16.7 16.6 15.5 48 46 44 41 7.05 6.54 6.61 6.09 18.2 16.3 16.9 15.3 48 43 46 41 11.6 13.0 12.9 13.2 67 62 70 64 14.3 13.7 12.5 13.5 63 68 66 68 11.4 11.1 13.1 15.4 63 67 69 71 0.3 0.3 0.5 0.8 0.5 0.5 0.3 0.5 0 1.5 0 1.5 0 0 0 0 0 0 0 0 0 0 0 0 FOR THREE MONTHS Pre-test 6.31 6.31 6.47 6.65 16.8 15.9 16.7 17.2 43 43 44 45 10.5 12.0 12.5 11.1 65 70 67 62 0.8 0.9 1.4 0.4 0 0 0 0 FEM Mo 1 6.61 5.86 6.46 5.85 16.3 15.7 17.3 14.9 44 43 47 40 12.2 10.6 13.2 11.1 72 66 69 62 0 0.5 0.8 0.8 0 0 0 0 ''^s_>' TABLE XIX (Continued) SUMMARY OF HEMATOLOGIC MEASUREMENTS ON DOGS FED Lymphocytes 7o Eosinophils % Monocytes 7, Basophils 7 /o Atypical Cells 7o Nucleated RBC's per 100 WBC's ppm in Diet 0 500 750 2,500 0 500 750 2,500 0 500 750 2,500 0 500 750 2,500 0 500 750 2,500 0 500 750 2,500 X i-^- L(oL- 27 31 30 29 5 4 3 3 1.3 1.4 1.6 0.1 0 0 0 0 0 0 0 0 0 0 0 0 MA.LES Months on Test 1 2 25 28 32 26 27 26 26 26 7 9 4 5 2 7 8 5 1.5 1.0 1.8 0.8 1.3 0.5 0.3 1.5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 26 27 24 21 11 6 6 6 0.8 0.5 0.8 0.5 0 0 0 0 0 0 0 0 0 0 0 0 ,FOR THREE MONTHS i-A-t; L-CO L- 25 25 27 28 7 2 3 8 1.9 1.9 1.0 1.4 0 0 0 0 0 0 0 0 0 0 0 0 Mo 1 25 30 25 29 3 3 6 7 0.3 0.8 0.5 1.5 0 0 0 0 0 0 0 0 0 0 0 0 TABLE XX SUMMARY OF URINALYSIS DATA,ON DOGS FE FOR THREE MONTHS Volume ml/24 hrs. Osmolality mOs/L Creatinine mg/100 ml Blood Number Positive Sugar Number Abnormal Acetone Number Positive Bilirubin Number Positive ppm in Diet 0 500 750 2,500 0 500 750 2,500 0 500 750 2,500 0 500 750 2,500 0 500 750 2,500 0 500 750 2,500 0 ' 500 750 2,500 | Pre-Test* 178 228 178 266 1549 1091 1612 1513 142 96 145 116 0 0 0 1 0 0 0 0 0 0 0 0 8 5 6 4 MALES Months on 1 2 135 209 134 163 1944 1482 1875 1500 179 114 137 98 0 0 165 180 114 159 1775 1790 1951 1651 173 153 179 158 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 3 3 4 4 4 2 3 Test 3 150 176 11.6 199 2004 1638 1906 1516 207 158 192 145 0 0 0 0 0 0 0 0 0 0 0 0 4 4 4 4 Pre-Test 159 169 153 203 1648 1366 1635 1527 177 130 151 119 0 1 0 0 0 0 0 0 0 0 0 0 2 0 3 0 FEMALES Month 1 126 140 150 209 1900 1 1639 1 1627 1456 175 113 112 106 0 0 0 0 0 0 0 0 0 0 0 0 3 1 3 0 Average of the measurement per dog for volume, osmolality, creatinine; number positive eight specimens for blood, sugar, acetone, bilirubin, protein, TABLE XX (Continued) SUMMARY OF URINALYSIS DATA ON DOGS FE Protein Number Abnormal Urobilinogen Erythrocytes per hpf Leucocytes per hpf Epithelial Cells per hpf Casts per Ipf ppm in Diet 0 500 750 2,500 0 500 750 2,500 0 500 750 2,500 0 500 750 2, 500 0 500 750 2,500 0 500 750 2,500 Pre-Test* 0 0 0 0 0.9 0.4 0.8 0.7 0 0 0 0 0-4 0-2 0-6 0-6 0-1 0-1 0 0 0 0 0 0 MALES Months on Test 1 2 3 0 0 0 0 0 0 0 0 0 0 0 0 1.0 0.8 0.8 0.6 0.6 0.8 0.8 0.6 1.0 0.6 0.8 0.8 0 0 0 0 0 0 0 0 0 0 0 0 0-3 0-1 0-1 0-1 0-2 0-4 0-10 0-5 0-3 0 0-1 0 0-1 0 0-1 0 0 0 0 0 0 0 0 0 0-1 0-10 0-5 0-3 0-1 0-1 0-2 0-2 0 0 0 0 OR THREE MONTHS Pre-Test 0 0 0 0 0.9 0.3 0.8 0.4 0 0 0 0 0-2 0-2 0-2 0-2 0-2 0-2 0-1 0-3 0 0 0 0 FEMALES Mont 1 0 0 0 0 1.0 1.0 1.0 0.6 0 0 0 0 0-8 0-2 0-1 0-1 0-3 0-5 0-1 0-1 0 0 0 0 TABLE XXI SUMMARY OF BIOCHEMICAL MEASUREMENTS ON DOGS FE Glucose mg % Urea Nitrogen mg % Cholesterol mg % Alkaline Phosphatase Bessey Units Transaminase Reitman-Frankel Units Total Protein g/100 ml ppm in Diet 0 500 750 2,500 0 500 750 2,500 0 500 750 2,500 0 500 750 2,500 0 500 750 2,500 0 500 750 2,500 J.L C L-C&L. 123 128 111 114 14 16 14 17 137 146 161 148 4.2 6.4 4.4 4.4 8 7 8 7 6.3 6.2 6.1 6.0 MALES Months on Test 1 2 3 137 125 116 140 126 112 111 116 109 101 117 107 14 15 14 22 19 20 21 17 16 23 21 19 133 150 138 140 174 163 184 206 189 181 206 188 3.2 6.9 6.6 10.7 17 14 15 11 3.3 8.1 6.9 11.3 15 15 18 15 3.0 8.2 5.3 11.7 13 11 17 13 6.1 6.2 6.4 6.0 6.2 6.2 5.9 6.1 6.2 6.2 6.3 6.3 OR THREE MONTHS 112 119 107 107 14 17 18 16 158 156 157 159 4.3 6.4 4.4 5.2 6 6 5 5 6.2 6.3 6.1 6.3 FEM M 1 108 112 95 96 21 21 23 21 164 180 209 277 3.9 8.4 6.2 10.5 9 12 10 13 6.0 5.8 6.1 6.4 TABLE XXI (Continued) SUMMARY OF BIOCHEMICAL MEASUREMENTS ON DOGS FE: OR THREE MONTHS Albumin/Globulin Albumin g/100 ml Creatinine mg % Bilirubin mg % ppm in Diet 0 500 750 2,500 0 500 750 2,500 0 500 750 2,500 0 500 750 2,500 .Ll.fc-L.fc-Ot^ 1.12 1.15 1.11 1.07 3.3 3.3 3.2 3.1 0.6 0.7 0.6 0.7 0.2 0.1 0.2 0.2 MALES Months on Test 1 2 1.27 . 1.16 1.25 1.05 3.4 3.2 3.3 3.1 0.6 0.6 0.5 0.6 0.4 0.4 0.3 0.3 0.97 1.15 1.18 0.98 3.0 3.3 3.3 3.1 0.8 0.8 0.7 0.7 0.3 0.2 0.2 0.2 3 1.00 1.03 1.02 1.13 3.2 3.1 3.1 3.3 0.8 0.9 0.9 0.8 0.2 0.2 0.2 0.2 I. L c" L cta L 1.11 1.14 1.19 1.16 3.2 3.3 3.3 3.3 0.7 0.7 0.7 0.7 0.1 0.1 0.1 0.2 FEM Mo 1 1.29 1.05 1.16 0.98 3.3 3.0 3.2 3.2 0.7 0.6 0.7 0.6 0.2 0.3 0.4 0.3 my TABLE XXII ORGAN WEIGHTS IN GRAMS OF INDIVIDUAL MALE DOGS SACRIFICED AFTER THREE MONTHS' FEEDING O Group I Control Dog No. 1158 1154 1162 1150 0^---- II 500 ppm 1153 - f11l 1165 1159 1155 1164 III R 750 Ppm- ^Z a 1160 1156 0 Iv IC^-- o 2500pDm | * >< -------------------- 01 S3 3 ^ N 0 P- a 0 t'O y> =1 0 L-< 0 0 3 5? 5' S-< (P y " 0 1163 1161 1151 1157 Body Wt, (kg) 12.0 11.5 11.0 8.0 11.5 9.9 14.9 10.0 8.2 9.8 11.8 8.6 10.3 9.0 11.0 11.0 VI U n) 4-1 C!4-iM)-<<cl!)(^U(U!>.^i-uf4in-)l i-i -! w (U 0) U 4-1 M ? rt a T-1 a X) m 0 sS ft h 9 ' rl a !-< a) H M h-i h-l W p-i ^ E-< P-1 90 106 127 397 30 40 64 25 11.8 73 102 175 332 27 32 70 19 7.0 74 111 132 301 25 29 69 24 5.0 77 76 95 270 24 25 47 14 4.6 73 108 174 408 36 25 73 24 5.3 71 90 144 400 26 29 59 21 4.9 85 143 191 593 22 32 77 32 8.0 75 74 153 370 20 25 70 14 8.9 69 68 120 320 15 32 57 17 6.4 82 95 121 357 24 29 55 17 5.9 91 88 157 454 29 26 59 17 11.2 85 60 121 309 23 18 46 17 7.3 84 84 113 486 24 25 68 25 6.2 81 80 113 493 20 18 62 19 3.4 75 87 149 541 22 22 60 20 3.3 90 101 197 516 29 32 63 16 4.3 n ,n a) u ^On)g 1-1 4J PQ M 5.2 110 6.9 95 6.3 92 4.6 86 5.6 93 5.0 93 8.8 128 4.9 86 6.2 74 4.8 80 4.9 108 3.2 78 5.6 99 4.4 87 5.6 108 4.0 99 M 3 f, ^ 9.5 8.3 5.8 9.2 36.3 13.7 13.7 4.3 8.8 13.2 9.6 8.7 11.0 9.8 16.6 6.7 TABLE XXIII ORGAN WEIGHTS IN GRAMS OF INDIVIDUAL FEMALE DOGS SACRIFICED AFTER THREE MONTHS' FEEDING Group I Control Dog No. 1137 1141 1145 1147 II ,, 500 p.pm 1135 | 1140 1143 B 1141 L-------- III 750 ppn^-. 1 " 1148 1139 1144 1136 IV 1146 r ^ i 2,500 ppm 1 3 1138 1142 1134 Body Wt. . (k). 11.4 11.7 9.2 9.6 8.3 7.5 8.8 7.6 10.8 8.6 9.4 15.3 6.8 9.2 8.6 6.8 W H) r-i c (U >> U 10 rt c M !-< 0) 1-1 0) n) j j r-i >-< 00 0) (U U 1-< 0 n 1 ^ 0) i-l a rf 4-1 t) n W i-l C/3 c^ l^i CO E-I < 78 107 172 349 25 24 57 103 6.2 1.1 76 107 161 372 23 20 66 111 14.5 1.8 75 65 140 347 19 26 46 83 7.6 1.7 65 94 152 308 28 31 62 92 12.3 2.0 68 81 114 356 22 22 54 87 5.3 1.0 65 60 98 274 22 21 43 76 10.2 1.0 81 70 121 397 19 30 46 88 17.6 1.4 75 78 134 357 15 22 42 80 4.4 1.6 74 74 126 352 16 21 44 77 11.4 1.5 78 73 . 138 416 23 30 60 83 11.7 1.8 60 67 118 350 17 21 47 75 6.9 1.3 84 99 189 533 30 30 69 117 13.9 1.5 72 55 90 342 11 21 39 76 6.3 1.2 82 80 139 520 17 24 54 80 11.7 1.8 76 79 134 430 21 24 53 76 9.0 1.2 73 67 30 351 12 22 42 70 10.2 1.7 TABLE. XXIV AVERAGE LIVER WEIGHTS AMD LIVER/BODY WEIGHT RATIOS OF DOGS FED B^ft------ MALES Final Liver ppm Body Weight Weight Liver Weight -, - Group Zonyl^ RP (kg) (g) Body Weight I 0 10.4 325 3.15 II 500 III' 750 IV 2,500 11.6 442 9.6 360 10.3 509 3.82 3.75 4.95 .-1 Final Body Weight (kg) 10.5 8.0 11.0 7.8 FEMALES Liver Weight (g) Live Body 344 346 413 411 0 0 -a OS -^ trt B> ^s Si" <n a a 0 t0 us 3 0 istt. so 0 ia ^^:y^ TABLE XXV Histopathology - Group I (Control) - H-7^47---------------- s^^^^^-s RESPIRATORY AND . CARDIOVASCULAR DIGESTIVE G 'C! OJ Q 0; ^! H 0 b0 n3 ^ a 4-' ^3 3 >h , w nj b-o)o&) osj tflo) ft tj Sex No. Dog ^ -P o3 ni n +3 a d) 3fl C P1 o H ^ u E! cij !-) 0 0 U H H a 3 c.s-, P( (U 0 [i; < 4-' 3 ro p (U 0 u u tfl nj co p^ 1150 d' A-. 1154 rf 1158 ^ ----.--------- ll62 ^ 1157 $ C+ c-.+----------.------- -- ll4l $ --------------.-------------------------------- es 1145 ? 0 1147 ^ -- -o ni 5 ~^ ------------- 0 . , - >h , id IB > taO ^ ^caoMa'dc ?-l S i-I.H a) != 0 I-IHpi^'br ec-OiiTx r-1 to (U B+ 0 3, Code: N' <D 0. A = Focal pleural fibrosis. B = Focal germinal cell atrophy or hypoplasia. C = Focal suppurativepneumonitis. 0 0 - = No abnormalities detected.. fl> tn + = Slight degree of lesion. 3 a 0 0 fl? 5" C/) 0 > 0 CD ia3 VOSi u..ei"oo |ou saoa -pe^ues ^^3 o & P 0 -hl4<^--l-\'-'nH-pl- -I'lh--p---''--'lH^-3 'hl-or-'u-'^ll0^---''0nlP-4v'il~vl---0n'' t|ao0uq o u H h^ SB V^OOV^OO\0\O<Y>O^COOVOOOVOOO-OP- (Tl ^; ca m c+ 0 P ^ 1-' !-' P rt- &'i-1- P- (D M 0' +o+o-to-K>o,,a,a,a,n>IM Pitui'bary CO I-' Thyroid. H- g- 0 1 ooo 1 Parathyroid. Adrenal I I I Skeletal Mus cle Sciatic Nerve Brain i Spinal Cord a o o CO S o a ^sr ' ' TO H g te. M C+ 0 d EB c+ C3' 0 O1 TO I'l^ !?1 ^ 0 p < 0 n o c+ rf ^ !- 0 3 CO 0. 00000 1 1 0 Mammary Gland Skin > ^ TO ^ W M H s! > > <^ M a 8 m Bone Marrow Spleen Mo Thymus hd iymph Node h3 TABLE XXVI Histopathology - Group IV - H-724' RESPIRATORY AND CARDIOVASCULAR DIGESTIVE ft 6 CD 3 (U U C o>u^ ij h s -p id ni w ho +3 a a ^ 0 0 a a 3 o P<ni(8 a &E-IW U H P-i^d.l Dog Nc'. Sex ^ fi 0 4^ d tU 0 < TO u c-> 1157 1151 rf'C+------- ll6l 1165 r i - - - - - - - - - 1154 --------------- 10' 1142 1146 v M C++------- S3 1158 '4 ' W ^ ^ C+ 0 ? $ --------- s' ?-------- (0 Code: C = Focal suppurative pneumonitis. . 0. B = Focal germinal cell atrophy or hypoplasia. a 0 E = Hepatocyte hypertrophy and degeneration. f0 - = No abnormalities detected. 0 + = Slight degree of lesion. of a ++ = Moderate degree of lesion. 0 3 S" 5" -w l 0 > 0 ..-3 !>a M h cfl ni 0) > ^ h H Q U r-f t9 ri (0 H CD M 0 F^ - - - - ; hb cfl h fS P & !> o H M i-l H D+ - D+ D+ D+ D+ - D+ D+ D+ GEN - ^-^ ra ? i-l >l +3M ' MCd (U> E-lO ( B+ B+ - -- ..OVOS."!..""'0"6'00TM"'"'8*""" How -' Hl-'l-'l-i HI-'PH o0 t-i i-. L_| i_i 0 0 H H H H H H H H 0 P- (D \_O^| O+C- ^-p(-\3v^+i -VC-M^C|-^'PV-IV^IQO^ 0 + 0 IM I II II II I M SSM ^0 0 & S? (B t-t- sn n K' 'i 01 o M PL p y p (D CD g l--" Oq {n EB ^ 4 P I-' 4 (D g. g ff 0 I-1 (D CQ H) sn co H- o' l-i [_. p, 0 n)CD(D^rj m rt- H- o (D ? o p o i-' P cr cn (D p. H PJ P LJ, , i_^ s p- i-'- n> ^iB c+ o +0+0+0+0 1111 0,0,^0^0, i i i i (T> ^ Pituitary 1111 1111 iiii 1111 Thyroid TPa-ralt^hy^ro.i^d. i i i i 1111 Adrenal iiii 1111 i i Skeletal Muscle bqmcisa-uHipc Nerve Brain Spinal Cord M o <~i ( H 1' 3g CQ ra o ix) ^ ^ o a M CO &a g i i i i i Eye |> M 00 0000 1 Mannnary 3 :& Gland 9 s> s TO 1111 iiii Skin Bone Marrow Spleen S Tbymus 3 i^ Lymph Node o s ^ 1^ (D S? & o TO .' 1-3 ^ > , ^ M 0 ^ -^ TABLE XXVII Histopathology - Group II - H-724? Dog No. 1155 1159 ll65 1153 1149 1145 1140 1155 Sex d" c? S S ? ? ? $ Liver - - Code: - a No abnormalities detected. Sigasnpan'Syanitized. Does not contain '!";>; ' ;;;:'. TA]BLE XXVIII Histopathc)logy - Group III - H-724? f i; Dog No. 1156 1152 ll6o 1164 Sex J' rf" S d Liver - 1156 ll44 1159 ll48 S - ? - 0 - ? Code: - a No abnormalities detected.. gfflpany Sanitized. Does not contain TSCA CBB APPENDIX I ' ompan^ Sanitized. Does not contain TSCA Cffi Dog No.: Sex: Group: 1150 Male Control Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. My el. Lymphocytes Eosinophils Monocytes Basophils Atypicals Nucleated RBC Biochemistry Glucose Urea Nitrogen Cholesterol Alk. Phos. GPT Total Protein A/G . Creatinine Bilirubin Albumin Urinalysis Volume Appearance Osmolality . Occult Blood Sugar Acetone pH Urobilinogen Bilirubin Protein Creatinine MICROSCOPIC RBC's WBC's Epithelial Bacteria Cast Sperm ULiJLMlUAL JLAgUKATORYDATAON INDIVIDUAL DOGS FEDf--------OR THREE MONTHS Coiitrol 6.56 16.1 40 8.1 63 62 1 0 24 10 2 1 0 0 6.84 17.4 45 8.5 52 52 0 0 44 4 0 0 0 0 118 22 110 2.9 6 6.4 1.10 0.6 0.3 3.7 185 D,Y,C1,P 1950 N N N 6.8 1.0 P Tr 132 0 0-2 0 2+ 0 0 119 17 130 3.0 5 6.3 1.28 0.8 0.4 3.5 160 D,Y,C1,P 1373 N N N 7.0 1.0 P Tr 108 0 1-4 0 2+ 0 1-5 Months on Te st 1 2 7.84 18.6 50 9.3 74 73 1 0 21 4 1 0 0 0 7.24 18.3 49 11.2 50 50 0 0 28 22 0 0 0 0 3 7.28 19.2 49 11.5 57 57 0 0 28 13 2 0 0 0 120 17 120 2.6 16 6.2 1.44 . 0.5 0.4 3.7 132 19 130 2.5 16 6.2 1.02 0.8 0.5 3.1 155 A,C1 2146 N Tr N 6.8 1.0 P 14- 150 0 '0-3 0 2+ 0 0 125 D,Y,C1,P 2159 N N N 6.8 1.0 P 1+ 166 0 0-1 0 2+ 0 2-6 134 13 120 2.3 14 6.1 0.97 0.7 0.2 3.0 110 D,Y,C1 2081 N N N 7.0 1.0 P 1+ 212 0 0 0-1 2+ 0 5-10 Company Sanitized. Does not contain TSCACB1 CLINICAL LABORATORY, FEDF .TA ON INDIVIDUAL DOGS 'OR THREE MONTHS Dog No.: Sex: Group: 1154 Male Control Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. My el. Lymphocytes Eosinophils Monocytes Basophils Atypicals Nucleated RBC k Coni:rol 7.28 18.7 51 15.0 74 71 3 0 22 4 0 0 0 0 7.24 18.1 48 11.5 70 70 0 0 24 6 0 0 0 0 Months on Tes t 1 2 8.04 19.4 54 12.5 67 67 0 0 25 7 1 0 0 0 7.88 19.0 49 12.1 63 63 0 0 32 5 0 0 0 0 3 7.64 19.2 51 11.7 68 66 2 0 23 9 0 0 0 0 Biochemistry Glucose Urea Nitrogen Cholesterol Alk. Phos. GPT Total Protein A/G Greatinine Bilirubin Albumin Urinalysis Vo lume Appearance Osmolality Occult Blood Sugar Acetone pH Urobilinogen Bilirubin Protein Greatinine MICROSCOPIC RBC's WBC's Epithelial Bacteria Cast Sperm 150 13 145 4.7 12 6.4 1.04 0.5 0.1 3.3 245 Y,C1,P 1357 N N N 7.0 1.0 P 1+ 130 0 0 0-1 2+ 0 0-1 124 10 175 4.9 5 6.6 1.07 0.8 0.1 3.4 275 Y,C1,P 1194 N N N 7.2 0.1 P N 114 0 0 0 1+ 0 0 180 14 145 3.4 20 6.2 1.17 0.6 0.4 3.3 126 10 160 3.8 13 6.3 1.14 0.8 0.2 3.4 124 12 150 3.3 12 6.7 1.24 0.8 0.2 3.7 205 A,C1 1740 N Tr N 7.0 1.0 P Tr 138 0 0 0-1 2+ 0 1-4 270 Y,C1,P 1142 N N N 7.2 0.1 N Tr 114 0 0 0 1+ 0 2-4 185 D,Y,C1,P 1845 N N N 7.2 1.0 P Tr 180 0 0 0-1 1+ 0 0 Company Sanitized. Does not confam T<^ Dog No.: Sex: Group: 1158 Male Control Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. Myel. Lymphocytes Eosinophils Monocytes Basophils Atypicals Nucleated RBC Biochemistry Glucose Urea Nitrogen Cholesterol Alk. Phos. GPT Total Protein A/G Creatinine Bilirubin Albumin Urinalysis Volume Appearance Osmolality Occult Blood Sugar Acetone pH Urobilinogen Bilirubin Protein Creatinine MICROSCOPIC RBC's WBC's Epithelial Bacteria Cast Sperm CLINICAL LABORATORY .DATA ON INDIVIDUAL DOGS FEDB^----------^OTHRREE MONTHS Con trol 6.56 16.1 42 9.9 67 67 0 0 27 4 2 0 0 0 6.32 16.4 44 9.8 72 72 0 0 24 3 0 1 0 0 116 14 135 3.6 8 5.7 1.19 0.4 0.1 3.1 95 A,C1,P 1929 N 1+ N 7.0 1.0 F 1+ 132 0 0-1 0 1+ 0 0 122 15 145 5.3 - 6.2 1.09 1.0 0.1 3.2 75 L,A,C1 1520 N Tr N 6.4 1.0 P Tr 260 0 0 0 1+ 0 0 Mionths on Tes t 1 2 7.00 16.8 46 9.4 62 62 0 0 32 2 4 0 0 0 7.00 17.4 47 16.9 68 67 1 0 26 3 3 0 0 0 3 6.48 17.4 47 10.9 73 73 0 0 21 6 0 0 0 0 134 16 135 2.9 15 6.0 1.09 0.6 0.3 3.1 123 19 165 3.3 15 6.2 0.73 0.8 0.3 2.6 101 18 150 2.9 14 6.2 0.75 0.8 0.3 2.7 50 A,C1 1919 N 1+ N 6.2 1.0 P 1+ 242 0 0-2 0 1+ 0 1-5 90 D,Y,C1 1908 N Tr N 6.4 1.0 P 1+ 222 0 0 0 2+ 0 0 95 D,Y,C1 1955 N N N 6.8 1.0 P Tr 262 0 0-1 0-1 2+ 0 4-5 ^Opipany Sanitized. Does not contain TSCA CB1 Dog No.: Sex: Group: 1162 Male Control Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. Myel. Lymphocytes Eosinophils Monocytes Basophils Atypicals Nucleated RBC Biochemistry Glucose Urea Nitrogen Cholesterol Alk. Phos. GPT Total Protein A/G Creatinine Bilirubin Albumin Urinalysis Volume Appearance Osmolality . Occult Blood Sugar Acetone PH Urobilinogen Bilirubin Protein Creatinine MICROSCOPIC RBC's WBC's Epithelial Bacteria Cast Sperm CLINICAL LABORATORY FEDf lATA ON INDIVIDUAL DOGS 'OR THREE MONTHS Coiitrol 6.08 15.3 40 14.6 67 67 0 0 27 2 4 0 0 0 6.08 15.6 40 16.8 67 65 2 0 23 8 2 0 0 0 Mon ths on Tes t 1 2 7.16 17.1 47 15.0 65 65 0 0 22 13 0 0 0 0 7.20 17.2 46 16.9 70 70 0 0 25 4 1 0 0 0 3 6.80 17.1 46 11.3 54 54 0 0 30 15 1 0 0 0 118 9 115 5.0 9 6.2 1.04 0.5 0.1 3.2 160 D,Y,C1,P 1556 N N" N 8.0 1.0 P 1+ 128 0 0-1 0 1+ 0 0 114 8 140 4.5 8 6.9 1.06 0.7 0.1 3.6 112 10 130 3.9 15 6.1 1.38 0.5 0.3 3.5 118 11 145 3.6 14 6.0 1.00 0.60.2 3.0 106 14 130 3.6 11 6.4 1.03 0.7 0.2 3.2 225 L,A,C1,P 1514 N N N 7.2 1.0 P Tr 132 0 0 0-1 2+ 0 0-1 130 A,C1,P 1971 N N N 7.0 1.0 P 1+ 184 0 1-3 1-3 1+ 0 0-2 175 Y,C1,P 1892 N M N 7.2 1.0 P 1+ 190 0 0 0 2+ 0 1-3 210 L,A,C1,P 2133 N N N 7.0 0.1 P 1+ 172 0 0 0-1 1+ 0 3-6 Gom^ Sanity. Does n^ contain TSCACBI Dog No.: Sex: Group: 1153 Male 500 ppm Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. Myel. Lymphocytes Eosinophils Monocytes Basophils Atypicals Nucleated RBC Biochemistry Glucose Urea Nitrogen Cholesterol Alk. Phos. GPT Total Protein A/G Greatinine Bilirubin AIbumin Urinalysis Vo lume Appearance Osmolality Occult Blood Sugar Acetone pH Urobilinogen Bilirubin Protein Creatinine MICROSCOPIC RBC's WBC's Epithelial Bacteria Cast Sperm ^^3 CLINICAL LAgORATOR^ATA ON INDIVIDUAL DOG!S FED------------TFHOEERE MONTHS Contbrol 7.00 17.3 48 13.3 66 66 0 0 30 4 0 0 0 0 7.68 18.8 50 11.1 60 60 0 0 34 2 4 0 0 0 Months on Tes t 1 2 7.84 18.7 52 11.0 62 61 1 0 33 2 3 0 0 0 8.08 19.7 53 15.4 62 61 1 0 30 8 0 0 0 0 3 7.60 18.7 50 9.9 63 62 1 0 28 7 2 0 0 0 116 13 140 6.4 9 6.2 1.39 0.4 0.1 3.6 245 Y,C1,P 605 N N N 8.0 0.1 N N 66 0 0 0 3+ 0 0 158 14 160 6.1 7 6.5 0.89 0.8 0.1 3.1 140 21 100 7.8 20 6.3 1.21 0.4 3.4 142 18 170 8.2 18 6.3 1.18 0.9 0.3 3.4 104 23 160 8.6 10 6.6 0.98 0.9 0.4 3.3 185 Y,C1,P 1268 N N N 7.0 1.0 P Tr 118 0 0-1 0 2+ 0 0 215 A,C1,P 1147 N ' N N 7.0 0.1 P Tr 88 0 0-1 0 3+ 0 1-5 110 Y,C1,P 1567 N M N 7.6 0.1 P 1+ 130 0 0 0 2+ 0 2-5 210 D,Y,C1,P 1331 N N N 6.8 1.0 P Tr 138 0 0 0-1 2+ 0 3-10 Company Sanitized. Does not contain TSCA CBS Dog No. : Sex: Group: 1155 Male 500 ppm Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. Myel. Lymphocytes Eosinophils Monocytes Basophils Atypical s Nucleated RBC Biochemistry Glucose Urea Nitrogen Cholesterol Alk. Phos. GPT Total Protein A/G Creatinine Bilirubin Albumin Urinalysis Volume Appearance Osmolality Occult Blood Sugar Acetone pH Urobilinogen Bilirutin Protein Creatinine MICROSCOPIC RBC's WBC's Epithelial Bacteria Cast Sperm U H f O R FED| CI,INICAL LABOEATORYBATA 01t INDIVIDUAL DOiGS TI1REE MONTHS Co ntrol ' 5.92 14.9 39 13.0 65 65 0 0 28 6 1 0 0 0 5.68 14.4 37 13.9 62 62 0 0 26 9 3 0 0 0 Months on Tes t 1 2 6.56 15.6 42 13.2 66 66 0 0 28 6 0 0 0 0 6.76 16.1 41 12.5 68 67 1 0 28 4 0 0 0 0 3 6.64 15.9 41 9.2 69 69 0 0 28 3 0 0 0 0 112 20 115 7.6 6 5.8 1.00 0.6 0.1 2.9 195 Y,C1,P 1478 N N N 7.0 0.1 N Tr 104 0 0 0-1 2+ 0 0 114 17 135 7.1 4 6.1 1.08 0.8 0.1 3.2 120 26 135 8.1 15 6.0 1.00 0.6 0.5 3.0 126 . 21 160 10.6 13 6.5 1.06 0.8 0.2 3.3 120 11 145 12.0 11 6.1 1.30 0.7 0.1 3.4 245 Y,C1,P 1273 N N . N 7.0 1.0 P N 92 0 0-2 0 2+ 0 0 125 A,C1,P 1819 N Tr N 7.0 1.0 N 1+ 124 0 0 0 1+ 0 0-1 185 D,Y,C1,P 1966 N N N 7.0 1.0 P 1+ 158 0 0 0 2+ 0 0 155 L,A,C1,P 2181 N N N 6.8 1.0 P 1+ 208 0 0 0 3+ 0 0-1 Company san,^. ^s not contain TSCACE, Dog No.: Sex: Group: 1159 Male 500 pom Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. Myel. Lymphbcytes Eosinophils Monocytes Basophils Atypicals Nucleated RBC C L I N I C AL F WLAB EDg T AOR ON INDIVIDUAL THEJ5E MONTHS DOG!-1 Con trol 5.64 15.2 40 11.0 72 71 1 0 27 1 0 0 0 0 5.60 15.3 39 12.3 60 60 0 0 32 5 3 0 0 0 Moiiths on Tes:t 1 2 5.84 14.9 39 12.6 60 58 2 0 31 5 4 0 0 0 5.48 14.3 36 13.7 75 75 0 0 17 6 2 0 0 0 2 5.60 14.3 36 12.9 71 70 1 0 23 6 0 0 0 0 Biochemistry Glucose Urea Nitrogen Cholesterol Alk. Phos. GPT Total Protein A/G Creatinine Bilirubin Albumin 116 18 165 6.6 7 6.2 0.92 0.5 0.1 3.0 124 17 175 6.4 6 6.3 1.48 0.9 0.1 3.8 118 17 175 7.5 9 6.1 1.40 0.5 0.2 3.6 118 17 195 8.7 13 6.0 1.13 0.8 0.1 3.2 109 19 185 8.3 12 6.1 0.93 0.9 0.2 2.9 Urinalysis Volume Appearance Osmolality Occult Blood Sugar Acetone pH Urobilinogen Bilirubin Protein Creatinine MICROSCOPIC RBC's WBC's i) Epithelial Bacteria Cast Sperm 350 L,Y,ci,P 560 N N N 6.2 0.1 N N 52 0 0 0 2+ .0 0 155 Y,C1,P 1121 N N N 7.2 1.0 P Tr 130 0 0 0 3+ 0 0 185 D,A,C1,P 1945 N Tr N 7.4 1.0 P 1+ 168 0 0 0-1 2+ 0 0-1 235 A,C1,P 1929 N Tr N 7.0 0.1 P Tr 182 0 0 0 2+ 0 0-1 275 L,A,C1,P 1656 N N N 7.0 1.0 P Tr 170 0 5-10 0-1 2+ 0 0-2 Sompany Sanitized. Does not contain TSCA CBI ^n Dog No.: Sex: Group: 1165 Male 500 ppm Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. Myel. Lymphocytes Eosinophils Monocytes Basophils Atypicals Nucleated RBC Biochemistry Glucose Urea Nitrogen Cholesterol Alk. Phos. GPT Total Protein A/G Creatinine Bilirubin Albumin Urinalysis Volume Appearance Osmolality Occult Blood Sugar Acetone pH Urobilinogen Bilirubin Protein Creatinine MICROSCOPIC RBC's WBC's Epithelial Bacteria Cast Sperm HHlFOR CLINICAL LABQEA.mE^ATA ON INDIVIDUAL DOG S FEDJ THE1EE MONTHS Coiitrol 4.96 13.2 33 10.4 60 60 0 0 38 1 0 1 0 0 5.92 15.7 41 16.2 64 64 0 0 35 1 0 0 0 0 134 12 - 6.5 12 6.2 1..11 - 0.2 3.3 200 L,A,C1,P 1283 N N N 7.2 0.1 P Tr 114 0 0-1 0-1 3+ 0 0 150 16 135 4.8 7 6.2 1.35 0.9 0.1 3.6 240 D,Y,C1,P 1136 N N N 6.8 0.1 P Tr 92 0 0 0 1+ 0 0 Months on Test 1 2 7.24 17.6 49 15.0 62 62 0 0 35 3 0 0 0 0 6.48 16.6 44 13.2 69 69 0 0 30 0 1 0 0 0 3 6.32 16.3 44 12.2 66 66 0 0 27 7 0 0 0 0 182 23 150 4.2 13 5.5 1.03 0.6 0.3 2.8 116 18 170 4.8 14 '6.1 1.22 0.8 0.2 3.4 116 25 160 4.0 12 6.1 0.91 0.9 0.2 2.9 310 Y,C1 1016 N N N 6.8 0.1 P N 76 0 0 0 3+ 0 0 190 A,C1,P 1698 N N N 7.0 1.0 P 1+ 142 0 0-1 0 2+ 0 0-3 65 Y,C1 1383 N N N 7.0 0.1 P Tr 116 0 0-3 0 2+ 0 0-1 Company Sanitized. Does nof contain TSCA CS( Dog No.: Sex: Group: 1152 Male 750 ppm Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. Myel. Lymphocytes Eosinophils Monocytes Basophils Atypicals Nucleated RBC Biochemistry Glucose Urea Nitrogen Cholesterol Alk. Phos. GPT Total Protein A/G Creatinine Bilirubin Albumin Urinalysis Volume Appearance Osmolality Occult Blood Sugar Acetone pH Urobilinogen Bilirubin Protein Creatinine MICROSCOPIC RBC's WBC's Epithelial Bacteria Cast Sperm FEWDJ T A CLINICAL LA "1- JL. J-JJ.^.L/.L^L j. \ ^ / ON INDIVIDUAL OR THEIEE MONTHS DC>GS Coiatrol 6.56 16.9 45 8.9 69 68 1 0 26 2 3 0 0 0 6.80 17.0 45 8.2 66 65 1 0 31 0 3 0 0 0 112 16 150 4.2 7 6.4 1.23 0.5 0.1 3.5 105 A,C1,P 1850 N N N 7.2 0.1 P 1+ 236 0 0 0 2+ 0 0 120 24 165 4.4 6 6.4 1.05 1.1 0.1 3.3 150 D,Y,C1,P 1987 N N N 7.0 1.0 P Tr 180 0 0 0 2+ 0 0-1 Mlonths on Tes t 1 2 7.04 17.1 48 10.9 78 78 0 0 22 0 0 0 0 0 7.36 17.9 49 13.6 74 74 0 0 25 1 0 0 0 0 3 7.08 17.8 49 11.8 68 68 0 0 28 1 3 0 0 0 116 28 185 8.1 15 6.1 1.55 0.6 0.2 3.7 120 23 215 8.3 21 6.3 1.28 0.9" 0.3 3.5 111 19 195 6.5 19 6.2 0.80 1.0 0.2 2.8 110 A,C1 2120 N Tr N 6.4 1.0 P Tr 180 0 0 0-1 2+ 0 0 135 D,Y,C1,P 2420 N ' Tr N 6.8 1.0 P 1+ 182 0 0-4 0 2+ 0 0-1 180 D,Y,C1,P 1861 N Tr N 7.0 1.0 P 1+ 180 0 0-1 0-2 2+ 0 0 Company Sanitized. Does not contain TSCA CBI Dog No.: Sex: Group: 1156 Male 750 ppm Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. My el. Lymphocyfces Eosinophils Monocytes Basophils Atypicals Nucleated RBC Biochemistry Glucose Urea Nitrogen Cholesterol Alk. Phos. GPT Total Protein A/G Creatinine Bilirutin Albumin Urinalysis Volume Appearance Osmolality Occult,Blood Sugar Acetone pH Urobilinogen Bilirubin Protein Creatinine MICROSCOPIC RBC's WBC's Epithelial Bacteria Cast Sperm CLINICAL LABORATORY .DATA ON INDIVIDUAL DOGS FEDl^^HHiFOR THREE MONTHS Coiitrol 5.68 14.1 36 11.1 66 65 1 0 32 2 0 0 0 0 6.12 15.9 42 10.5 64 64 0 0 34 1 1 0 0 0 MOIaths on Tes t 1 2 6.56 16.1 43 12.9 61 61 0 0 32 3 4 0 0 0 6.52 15.7 42 11.4 57 57 0 0 29 12 2 0 0 0 3 6.40 16.0 43 11.7 65 65 0 0 26 9 0 0 0 0 106 17 160 3.7 7 5.9 0.93 0.6 0.1 2.8 140 Y,C1,P 1924 N Tr N 6.8 1.0 N Tr 138 0 2-6 0 2+ 0 1-2 104 15 180 3.5 4 6.0 0.79 0.9 0.1 2.6 110 24 180 3.7 16 5.6 1.09 0.5 0.5 2.9 104 19 190 4.2 17 5.7 1.09 0.7 0.1 3.0 109 15 180 3.3 18 5.9 1.27 0.9 0.1 3.3 255 L,A,C1,P 1777 N N N 7.6 0.1 P Tr 116 0 0 0 2+ 0 0-1 125 A,C1,P 2045 N Tr N 6.8 1.0 P 1+ 130 0 0-2 0-1 1+ 0 0 90 A,C1,P 1971 N Tr N 7.0 1.0 P 1+ 196 0 0-2 0 2+ 0 4-8 90 D,Y,C1 2207 N Tr N 6.8 1.0 P 1+ 224 0 2-5 0-1 2+ 0 1-6 Company Sanitized. Does not contain TSCA CBE Dog No. : Sex: Group: 1160 Male 750 ppm Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. Myel. Lymphocytes Eosinophils Monocytes Basophils Atypicals Nucleated RBC Biochemistry Glucose Urea Nitrogen Cholesterol Alk. Phos. GPT Total Protein A/G Creatinine Bilirubin Albumin Urinalysis Volume Appearance Osmolality Occult Blood Sugar Acetone pH Urobilinogen Bilirubin Protein Creatinine MICROSCOPIC RBC's WBC's Epithelial Bacteria Cast Sperm FEWEJ T A CLINICAL L 0-L X -t-lXIJL^ ^.^ J ^ J- J 01? INDIVIDUAL OR TI1REE MONTHS DCGS C(3ntrol 5.92 15.5 39 8.1 69 69 0 0 29 1 1 0 0 0 5.68 15.7 42 8.5 67 67 0 0 30 2 1 0 0 0 Months on Tes t 1 2 6.20 16.3 43 9.6 74 73 1 0 25 0 1 0 0 0 6.44 16.9 42 8.9 70 70 0 0 29 1 0 0 0 0 3 6.40 17.2 46 14.4 80 80 0 0 18 2 0 0 0 0 108 11 160 5.2 . . 8 5.8 1.23 0.4 0.1 3.2 205 Y, C1,P 1215 N N N 7.0 0.1 P Tr 112 0 0 0 1+ 0 0-1 112 6 180 5.3 6 6.4 1.62 0.7 0.1 4.0 104 19 200 7.2 13 5.8 1.06 0.4 0.2 3.0 112 15 230 8.0 16 6.2 1.22 0.7 0.2 3.4 104 20 200 6.1 16 6.3 0.88 0.8 0.2 2.9 170 Y,C1,P 1289 N N N 7.0 1.0 P Tr 130 0 0 0 2+ 0 0 115 A,C1 1992 N Tr N 6.8 0.1 P Tr 140 0 0-1 0 2+ 0 0-2 70 D,Y,C1,P 1877 N Tr N 7.2 0.1 P Tr 196 0 0 0 2+ 0 0 130 L,A,C1,P 2151 N N N 6.8 1.0 P 1+ 180 0 0 0-1 2+ 0 0-1 ^mpany'SsnWT'p^?o w^ cwtatn TSCA cs? AJ. 1. J-JJ.1U J^tl. "------I1 CLINICAL LABORAT: ORY DA.TA ON INDIVIDUAL FE: 'OR THREE MONTHS DOGS Dog No.: Sex: Group: 1164 Male 750 ppm Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. My el. Lymphocytes Eosinophils Monocytes Basophils Atypicals Nucleated RBC Con trol 6.24 16.8 44 17.1 59 59 0 0 32 5 4 0 0 0 5.76 15.1 40 16.3 62 62 0 0 29 9 0 0 0 0 Biochemistry Glucose Urea Nitrogen Cholesterol Alk. Phos. GPT Total Protein A/G Creatinine Bilirubin Albumin 114 11 140 4.2 8 5.8 1.04 0.4 0.1 3.0 114 9 155 4.4 15 6.3 1.00 0.8 0.1 3.2 Urinalysis Volume Appearance Osmolality Occult Blood Sugar Acetone PH Urobilinogen Bilirutin Protein Creatinine MICROSCOPIC RBC's WBC's Epithelial t) Bacteria Cast Sperm 225 D,Y,C1,P 1373 N N N 7.0 0.1 P Tr 132 0 0 0 140 0 170 Y,C1,P 1478 N N N 7.4 1.0 N N 118 0 0 0 240 0-1 Months on Tes t 1 2 6.40 15.7 42 18.3 67 67 0 0 27 6 0 0 0 0 6.28 16.0 42 16.2 66 64 2 0 20 14 0 0 0 0 3 6.56 16.7 44 14.6 64 64 0 0 25 11 0 0 0 0 114 12 170 7.4 14 6.1 1.29 0.5 0.2 3.4 127 12 190 7.2 17 6.1 1.12 0.6 0.1 3.2 110 11 180 5.3 15 6.2 1.13 0.7 0.1 3.3 185 Y,C1,P 1341 N Tr N 7.0 1.0 P 14- 96 160 D,Y,C1,P 1535 N N N 7.2 0.1 P Tr 142 65 Y,C1 1404 N N N 6.8 1.0 P 14- 182 0 0-1 <- u 340 0-1 --. "-- -a 0 0 0 0-1 0 0-1 24- 44- 0 0 mae tint COitiialnTSSACB? ru; s. i-in.ujL.fi. x ^ ^--f} CLINICAL LA,BOEATOKY^IAIA ON INDI;VIDUAL FED I H|U'OR THREE V[ONTHS EIOGS Dog No.: Sex: Group: 1151 Male 2500 ppm Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. Myel. Lymphocytes Eosinophils Monocytes Basophils Atypicals Nucleated RBC Biochemistry Glucose Urea Nitrogen Cholesterol Alk. Phos. GPT Total Protein A/G Creatinine Bilirubin Albumin Urinalysis Volume Appearance Osmolality Occult Blood Sugar Acetone pH Urobilinogen Bilirubin Protein Creafcinine MICROSCOPIC RBC's WBC's epithelial Bacteria Cast Sperm Coiitrol 6.64 17.2 46 13.0 72 71 1 0 23 5 0 0 0 0 6.48 17.3 45 12.1 62 62 0 0 37 1 0 0 0 0 92 18 150 5.5 5 6.6 1.07 0.4 0.2 3.4 260 L,A,C1,P 1651 N N N 7.0 0.1 P Tr 128 0 0 0 2+ 0 0 114 14 155 5.4 4 6.1 1.04 0.8 0.1 3.1 130 D,Y,C1,P 1745 N N N 7.6 1.0 P Tr 154 0 0-1 0 2+ 0 0 Months on Tes1t 1 2 7.00 16.5 45 16.7 67 64 3 0 26 7 0 0 0 0 6.68 16.4 43 16.5 67 67 0 0 27 5 1 0 0 0 3 6.20 16.3 43 21.9 80 77 3 0 14 5 1 0 0 0 94 21 185 10.8 10 5.9 1.05 0.6 0.2 3.0 108 24 230 13.6 12 6.3 0.78 0.7 0.1 2.8 102 10 200 12.2 9 6.2 1.21 0.7 0.1 3.4 105 Y,C1 1730 N N N 7.0 1.0 P Tr 108 0 0-1 0 3+ 0 0-1 125 & ("1 V ""'-- 1908 K N N 7.0 1.0 P Tr 168 0 0 0 2+ 0 0-1 100 D,Y,C1,P 1824 N N N 6.8 1.0 P Tr 220 0 0-3 O--ii 4+ 0 0 'Bpai^^aniE&aef. Hoss R5? eanfaTn TS'CACB? Dog No.: Sex: Group: 1157 Male 2500 ppm Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. My el. Lymphocytes Eosinophils Monocytes Basophils Atypicals Nucleated RBC Biochemistry Glucose Urea Nitrogen Cholesterol Alk. Phos. GPT Total Protein A/G Creatinine Bilirubin Albumin Urinalysis Volume Appearance Osmolality Occult Blood Sugar Acetone pH Urobilinogen Bilirubin Protein Creatinine MICROSCOPIC RBC's WBC's Epithelial Bacteria Cast Sperm CLINICAL LA?ORATQRYJ^TAON INDIVIDUAL DOiSS FED?IIHBfOR TffiIEE MONTHS Con fcrol 6.68 16.9 44 12.8 78 78 0 0 22 0 0 0 0 0 6.48 16.5 44 10.8 73 72 1 0 25 2 0 0 0 0 M(anths on Tes t 1 2 6.92 16.8 45 11.8 67 67 0 0 26 7 0 0 0 0 6.56 16.0 42 13.1 73 73 0 0 20 4 3 0 0 0 3 5.76 15.1 40 13.8 68 68 0 0 23 9 0 0 0 0 116 25 145 4.1 10 5.8 1.03 0.6 0.1 2.9 240 A,C1,P 2138 N N N 7.2 0.1 P Tr 136 0 0 0 2+ 0 0 114 18 145 4.2 4 5.9 1.02 0.9 0.1 3.0 94 27 170 12.6 11 6.3 1.03 0.6 0.3 3.2 122 17 185 10.1 14 6.0 1.11 0.7 0.1 3.2 103 22 170 11.7 12 6.2 1.08 0.7 0.1 3.2 150 Y,C1,P 1619 N N N 6.8 0.1 P N 122 0 0 0-1 1+ 0 0 175 A,C1,P 1961 N N N 7.0 0.1 N Tr 130 0 0-1 0 2+ 0 1-3 90 A,C1,P 1814 N N' N 7.2 1.0 P Tr 226 0 0-1 0 3+ 0 0-1 180 D,Y,C1,P 1567 N N N 7.2 1.0 P Tr 144 0 0-2 0-1 3+ 0 0-4 company Sariiiized. Does not contain TSCA CBi CLINICAL LABORATORYJjATAOK[ INDIVIDUAL DO'GS FEDHl HH|fOR THEEE MONTHS Dog No.: Sex: Group: 1161 Male 2500 ppm Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. My e l . Lymphocytes Eosinophils Monocytes Basophils Atypicals Nucleated RBC Biochemistry Glucose Urea Nitrogen Cholesterol Alk. Phos. GPT Total Protein A/G Creatinine Bilirubin Albumin Urinalysis Volume Appearance Osmolality Occult Blood Sugar Acetone pH Urobilinogen Bilirubin Protein Creatinine MICROSCOPIC RBC's WBC's Epithelial Bacteria Cast Sperm Cointrol 6.64 17.0 44 8.3 61 61 0 0 36 3 0 0 0 0 6.60 16.8 44 8.9 56 56 0 0 35 8 1 0 0 0 119 20 130 3.7 9 6.3 1.08 0.5 0.1 3.3 140 D,Y,C1,P 1961 P N N 6.8 1.0 N Tr 156 0 0-1 0 2+ 0 0-1 120 12 140 3.6 8 6.1 1.18 0.7 0.1 3.3 120 L,A,C1,P 2272 N N N 7.0 1.0 N Tr 174 0 2-6 0 1+ 0 0 M onths o-n Tes;t 1 2 6.68 15.8 42 12.3 66 64 2 0 22 11 1 0 0 0 6.12 15.1 39 10.8 65 65 0 0 32 3 0 0 0 0 3 6.52 15.7 42 11.6 67 67 0 0 27 5 1 0 0 0 105 30 160 8.6 12 6.2 1.08 0.6 0.3 3.2 112 22 190 8.7 17 6.4 1.09 0.7 0.2 3.3 100 27 180 10.1 14 6.4 1.28 0.8 0.2 3.6 180 A,C1 1955 N N N 6.8 1.0 P Tr 120 0 2-10 0-1 2+ 0 0-2 150 D,Y,C1,P 2164 N N N 7.0 1.0 P 14- 152 0 1-5 0 2+ 0 0 170 D,Y,C1 2103 N N N 6.8 1.0 P Tr 150 0 0-3 0-2 2+ 6 5-10 ompany Sanitized. Does not contain TSCA CBS CLINICAL IABORATOB FEDl IATA ON INDIVIDUAL DOGS |FOR THREE MONTHS Dog No.: Sex: Group: 1163 Male 2500 ppm Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. Myel. Lymphocytes Eosinophils Moriocytes Basophils Atypicals Nucleated RBC Biochemistry Glucose Urea Nitrogen Cholesterol Alk. Phos. GPT Total Protein A/G Greatinine Bilirubin Albumin Urinalysis Volume Appearance Osmolality Occult Blood Sugar Acetone pH Urobilinogen Bilirubin Protein Greatinine MICROSCOPIC RBC's WBC's Epithelial Bacteria Cast Sperm C ontrol 5.12 13.1 33 12.4 76 75 1 0 23 1 0 0 0 0 5.24 13.7 35 12.8 67 66 1 0 27 6 0 0 0 0 112 13 150 5.0 9 5.6 0.98 - 0.1 2.8 630 L,Y,C1,P 248 N N N 7.0 0.1 N N 18 0 0-5 0 4+ 0 0 124 12 165 3.8 9 5.9 1.18 0.7 0.1 3.2 460 L,Y,C1,P 471 N N N 7.0 0.1 N N 38 0 0-2 0 3+ 0 0 Months on Test 1 2 5.76 13.8 36 11.8 63 62 1 0 31 6 0 0 0 0 6.08 14.6 38 13.4 68 68 0 0 24 6 2 0 0 0 3 5.88 14.2 37 14.1 73 70 3 0 21 6 0 0 0 0 110 14 210 10.8 It- 6.2 1.02 0.6 0.2 3.1 127 ' 20 220 12.7 16 6.4 0.94 0.8 0.5 3.1 122 18 200 12.8 15 6.3 0.95 0.8 0.5 3.1 190 L,Y,C1 352 N N N 7.6 0.1 N N 34 0 0-1 0-1 4+ 0 0 270 Y,C1,P 718 N N N 7.2 0.1 N Tr 84 0 0-2 0 3+ 0 0 345 Y,C1,P 570 N ' N N 7.0 0.1 P N 66 0 0 0-1 4+ 0 0 Company Sanitized. Does not contain TSCA CB5 Dog No.: Sex: Group: 1137 Female Control Henatology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. My el. Lymphocytes Eosinophils Monocytes Basophils Atypicals Nucleated RBC ^ q L.liliMlL.AL 1Ai(BULiU, K^miy^)AlA FED' |FOR UM iNUiViUUAL THREE MONTHS UUUS C<3ntrol 6.72 17.8 47 12.4 60 58 2 0 28 12 0 0 0 0 6.68 18.3 48 11.4 62 62 0 0 25 10 3 0 0 0 M[onths on Test 1 2 7.40 18.5 51 13.1 76 76 0 0 20 4 0 0 0 0 7.28 18.9 51 10.6 71 71 0 0 24 5 0 0 0 0 3 7.76 19.7 54 9.1 66 66 0 0 25 9 0 0 0 0 Biochemistry Glucose Urea Nitrogen Cholesterol Alk. Phos. GPT Total Protein A/G Greatinine Bilirubin Albumin Urinalysis Volume Appearance Osmolality Occult Blood Sugar Acetone PH Urobilinogen Bilirubin Protein Creatinine MICROSCOPIC RBC's WBC's Epithelial Bacteria ^\ Cast 114 10 160 4.5 7 6.4 1.20 0.5 0.1 3.5 125 D,Y,C1,P 1359 N N N 8.5 1.0 P Tr 146 0 0 0-1 3+ 0 109 12 150 4.7 3 5.5 1.15 0.5 0.2 2.9 235 D,Y,C1,P 1651 N N N 6.8 1.0 N Tr 114 0 0-1 0 1+ 0 111 16 160 4.1 8 6.3 1.50 0.5 0.2 3.8 165 A,C1,P 2142 N Tr N 6.8 1.0 P Tr 164 0 2-8 0 240 110 19 185 3.9 9 -6.4 1.21 0.7 0.2 3.5 101 26 165 10.1 15 6.7 1.06 0.8 0.2 3.4 190 A,C1,P 2013 M M N 7.0 1.0 P Tr 168 0 0 0 2+ 0 140 A,C1,P 1646 N N N 6.8 1.0 P 1+ 156 0 0 0 2+ 0 Company Sanitized. Poes not contain TSCACBl Dog No.: Sex: Group: 1141 Female Control Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. My el. Lymphocytes Eosinophils Monocytes Basophils Atypicals Nucleated KBC Biochemistry Glucose Urea Nitrogen Cholesterol Alk. Phos. GPT Total Protein A/G Creatinine Bilirubin Albumin Urinalysis Volume Appearance Osmolality Occult Blood Sugar Acetone pH Urobilinogen Bilirubin Protein Creatinine MICROSCOPIC RBC's WBC's Epithelial Bacteria Cast HHHFOR CLINICAL L^BDgAjgflR^tATA 01(T INDIVIDUAL Dl3GS FEDl TIffiEE MONTHS C(mtrol 6.52 17.5 45 10.4 72 72 0 0 21 6 1 0 0 0 6.60 17.6 46 11.1 74 71 3 0 15 8 3 0 0 0 Ilonths on Test 1 2 6.00 14.9 39 9.0 73 73 0 0 26 1 0 0 0 0 6.64 17.2 45 10.8 77 75 2 0 20 2 1 0 0 0 3 6.28 16.2 43 7.8 65 65 0 0 27 6 2 0 0 0 114 10 145 5.0 10 6.3 0.92 0.5 0.1 3.0 250 D,Y,C1,P 1546 N N N 7.0 1.0 N Tr 144 0 0-1 0-2 2+ 0 112 15 140 5.3 3 6.5 0.95 0.7 0.3 3.2 190 L,A,C1,P 2018 N N' N 6.6 1.0 N Tr 152 0 0 0-1 1+ 0 112 17 190 4.1 12 6.3 1.10 0.6 0.2 3.3 147 11 160 3.6 19 6.3 1.23 0.9 0.1 3.5 112 16 145 4.3 13 6.2 1.50 0.8 0.1 3.7 150 D,Y,C1,P 1625 N Tr N 7.0 1.0 N Tr 142 0 0 0-3 3+ 0 85 D,Y,C1,P 1924 N N N 8.0 1.0 P Tr 230 0 0-1 0-1 3+ 0 175 A,C1,P 1856 N N N 7.0 1.0 N Tr 174 0 0-2 1-5 2+ 0 Company Sanded. Doss not contain TSCA C61', Dog No. : Sex: Group: 1145 Female Control Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. Myel. Lymphocytes Eosinophils Monocytes Basophils Atypicals Nucleated RBC Biochemistry Glucose Urea Nitrogen Cholesterol Alk. Phos. GPT Total Protein A/G Greatinine Bilirutin Albumin Urinalysis Volume Appearance Osmolality Occult Blood Sugar Acetone pH Urobilinogen Bilirubin Protein Greatinine MICROSCOPIC RBC's WBC's Epithelial Bacteria Cast CLINICAL LABO; RATORYJ2ATAON INDIVIDUAL EIOGS FED|----------FORTHREE MONTHS ^^^^ys Coinitrol 7.08 16.9 43 9.2 74 74 0 0 24 2 0 0 0 0 6.72 16.0 41 11.2 64 63 1 0 30 3 3 0 0 0 106 13 155 3.5 7 5.9 1.16 0.7 0.6 3.2 190 Y,C1,P 1289 N N N 7.6 1.0 N N 128 0 0 0-1 2+ 0 118 11 175 3.4 6 5.9 1.47 0.8 0.2 3.5 80 A,C1,P 2303 N N N 8.0 1.0 N Tr 236 0 0-1 0 1+ 0 Months on Tes t 1 2 6.88 16.2 43 9.5 78 78 0 0 20 2 0 0 0 0 7.20 17.8 47 12.6 74 74 0 0 26 0 0 0 0 0 3 6.76 16.8 42 8.3 66 65 1 0 33 1 0 0 0 0 110 14 155 3.5 10 5.4 1.58 0.6 0.2 3.3 118 15 160 3.2 17 5.7 0.99. 0.8 0.2 2.8 102 21 295 3.1 13 6.5 1.32 1.0 0.2 3.7 110 A,C1,P 1824 N Tr N 7.2 1.0 P Tr 186 0 0 0-1 2+ 0 120 D,Y,C1,P 1871 N N N 7.0 1.0 P 1+ 196 0 0 0-1 2+ 0 175 L,A,C1,P 1735 N Tr N 7.0 1.0 P 1+ 180 0 0 0 1+ 0 ipany Sanitized. Does not contain TSCA CBB Dog No.: Sex: 1147 Female Control Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. My el. Lymphocytes Eosinophils Monocytes Basophils Atypicals Nucleated RBC Biochemistry Glucose Urea Nitrogen Cholesterol Alk. Phos. GPT Total Protein A/G Creatinine Bilirubin Albumin Urinalysis Volume Appearance Osmolality Occult Blood Sugar Acetone pH Urobilinogen Bilirubin Protein Creatinine MICROSCOPIC RBC's WBC's Epithelial Bacteria Cast CLINICAL LABORATORYDATA 0]N INDIVIDUAL D(3GS FED^| ttrilpOR T:HREE MONTHS ^^^^^^B C(Dntrol 6.32 16.1 43 11.8 61 61 0 0 27 11 1 0 0 0 6.52 17.1 44 11.5 60 60 0 0 30 6 4 0 0 0 t tenths on Tes;t 1 2 6.16 15.5 43 17.2 60 60 0 0 34 5 1 0 0 0 6.60 17.1 45 15.2 66 66 0 0 28 5 1 0 0 0 3 5.84 17.4 46 12.8 60 59 1 0 24 16 0 0 0 0 114 20 170 4.4 8 6.8 1.04 0.8 0.1 3.5 150 Y,C1,P 1073 N N N 6.8 0.1 P Tr 152 0 0-2 0-1 2+ 0 110 22 170 3.9 5 6.4 1.02 1.1 0.1 3.2 50 D,Y,C1 1945 N N N 7.0 1.0 N Tr 340 0 0 0-2 2+ 0 100 36 150 4.0 6 5.8 0.98 1.0 0.2 2.9 116 41 165 3.6 10 5.7 0.94 1.2 0.1 2.8 109 46 175 3.1 8 5.8 1.27 1.8 0.1 3.2 80 D,Y,C1 2008 N Tr N 6.4 1.0 P Tr 208 140 D,Y,C1 1367 N N N 7.2 0.1 P Tr 138 110 D,Y,C1 1499 N N N 7.0 1.0 P Tr 170 0 0 0 0 0 0 0 0 0 1+ 2+ 2+ 0 0 0 Company Sanitized. Does not contain TSCA CBB: Dog No.: Sex: Group: 1135 Female 500 ppm Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. My el. Lymphocytes Eosinophils Monocytes Basophils Atypicals Nucleated RBC Biochemistry Glucose Urea Nitrogen Cholesterol Alk. Phos. GPT Total Protein A/G Creatinine Bilirubin Albumin Urinalysis Volume Appearance Osmolality Occult Blood Sugar Acetone pH Urobilinogen Bilirubin Protein Creatinine MICROSCOPIC RBC's WBC's Epithelial Bacteria Cast FEQ^I ^uj.isi-uAJ-1 J-A^m.AlUR^ATA ON ][NDIVIDUAL BHIf01^THRJEiE MONTHS DlDGS Coni:rol 7.04 18.8 49 10.9 66 66 0 0 32 1 1 0 0 0 5.68 14.6 47 11.2 72 71 1 0 25 0 3 0 0 0 102 13 130 7.0 6 6.7 1.40 0.5 0.2 3.9 60 D,Y,C1,P 1871 N Jr N 6.6 0,1 N Tr 202 0 0-1 0-2 1+ 0 110 8. 150 6.6 2 5.8 1.09 0.5 0.2 3.0 165 Y,C1,P 1136 N N N 7.0 0.1 N Tr 88 0 0-2 0 1+ 0 Itonths on Test 1 2 7.08 17.8 48 11.4 71 69 2 0 24 5 0 0 0 0 7.24 18.0 48 12.4 74 72 2 0 24 2 0 0 0 0 3 6.64 16.5 43 11.2 60 58 2 0 22 16 2 0 0 0 118 11 205 8.0 9 5.8 1.05 0.6 0.3 3.0 104 23 235 6.5 10 6.6 0.90 0.7 0.4 3.1 110 22 285 4.2 9 6.8 0.80 0.8 0.3 3.0 160 A,C1,P 1467 N 1+ N 8.0 1.0 P 1+ 118 0 0-1 0-5 2+ 0 160 D,Y,C1,P 1488 N ' Tr N 7.0 1.0 N Tr 154 0 0 0-1 2+ 0 145 A,C1 2098 N N N 6.8 1.0 P Tr 182 0 0 0 1+ 0 fwompanySanitized. Does not confcSih TiSCA CffP Dog No.: Sex: Group: 1140 Female 500 ppm Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. Myel. Lymphocytes Eosinophils Monocytes Basophils Atypicals Nucleated PJ3C Biochemistry Glucose Urea Nitrogen Cholesterol Alk. Phos. GPT Total Protein A/G. Creatinine Bilirubin Albumin Urinalysis Volume Appearance Osmolality Occult Blood Sugar ' Acetone pH Urobilinogen Bilirubin Protein Creatinine MICROSCOPIC RBC's WBC's Epithelial Bacteria Cast r^^^ CLINICAL IA()RTORYJ)ATA OKI INDIVIDUAL DOG S FEDH^^------FOTRt[REE MONTHS Coritrol 6.20 15.5 41 8.2 62 61 1 0 34 3 0 1 0 0 6.40 16.5 44 7.3 72 71 1 0 26 2 0 0 0 0 Months on Te;st 1 2 6.44 16.4 45 10.5 61 61 0 0 33 4 2 0 0 0 6.68 17.1 45 11.1 69 69 0 0 26 5 0 0 0 0 3 6.16 15.7 41 7.7 68 68 0 0 25 7 0 0 0 0 119 10 170 10.7 7 6.1 1.25 0.8 0.1 3.4 485 L,Y,C1,P 209 N N N 7.2 0.1 N N 14 0 0-2 0-2 3+ 0 122 9 170 10.9 3 6.0 1.30 0.6 0.1 3.4 120 17 165 8.3 10 5.9 1.15 0.5 0.3 3.2 110 22 170 6.9 9 6.1 1.12 0.6 0.3 3.2 117 17 200 4.7 6 6.1 1.54 0.7 0.3 3.7 165 Y,C1,P 951 N N N 8.0 0.1 N N 74 0 0 0-1 2+ 0 100 D,Y,C1 1310 N Tr N 7.2 1.0 N Tr 102 90 A,C1 2151 N Tr N 7.2 1.0 P 1+ 204 85 D,Y,C1,P 1814 N N N 7.2 1.0 P 1+ 196 0 0 0 0-2 0-1 0 0 0 0-1 1+ 1+ 2+ 0 0 0 ,.^CB ,, A noes"n0oteonia'" ^antt^. 0" |l Dog No.: 1143 & Sex: Female v Group: 500 ppm ^IP\ ^^ Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. Myel. Lymphocytes Eosinophils Monocytes Basophils Atypicals Nucleated RBC Biochemistry Glucose Urea Nitrogen Cholesterol Alk. Phos. GPT Total Protein A/G Creatinine Bilirubin Albumin Urinalysis Volume Appearance Osmolality Occult Blood Sugar Acetone pH Urobilinogen Bilirubin Protein Creatinine MICROSCOPIC RBC's WBC's Epithelial Bacteria Cast f F O R FED------ CLINICAL LABORSLTORYMTA ON INDIVIDUAL DOC5S THREE MONTHS Contrc)1 5.84 14.3 36 16.1 70 68 2 0 24 1 5 0 0 0 6.28 15.8 41 14.8 74 73 1 0 17 5 4 0 0 0 Mernths on Tes t 1 2 . 5.72 13.8 37 11.3 68 68 0 0 30 2 0 0 0 0 6.40 15.9 40 15.5 60 60 0 0 31 9 0 0 0 0 3 5.92 15.7 41 11.3 67 67 0 0 29 4 0 0 0 0 116 11 145 3.7 7 6.1 1.12 0.5 0.1 3.2 150 Y,C1,P 1205 N N N 7.0 1.0 N Tr 144 0 0 0 2+ 0 112 12 160 4.7 4 6.4 1.05 0.8 0.1 3.3 93 32 170 6.5 11 5.7 0.97 0.6 0.3 2.8 106 32 200 5.4 9 6.6 0.99 0.7 0.8 3.3 109 33 220 4.1 11 6.6 1.34 0.9 0.9 3.8 140 Y,C1,P 1079 N N N 6.8 1.0 N Tr 146 0 0 0-1 1+ 0 170 D,Y,C1 1320 N N N 6.8 1.0 N Tr 102 0 0-1 0 3+ 0 150 Y,C1,P 1714 N N N 6.8 1.0 P Tr 146 0 0 0 4+ 0 205 D,Y,C1,P 1252 N N N 7.0 0.1 P Tr 114 0 0 0 1+ 0 Saanpany Sanitized. Does not contain TSCA CB1 Dog No.: Sex: Group: 1149 Female 500 ppm Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. My el. Lymphocytes Eosinophils Monocytes Basophils Atypicals Nucleated KBC Biochemistry Glucose Urea Nitrogen Cholesterol Alk. Phos. GPT Total Protein A/G Creatinine Bilirubin Albumin Urinalysis Volume Appearance Osmolality Occult Blood Sugar Acetone pH Urobilinogen Bilirubin Protein Creatinine MICROSCOPIC RBC's WBC's Epithelial Bacteria Cast m i f O R LiijilNiUAL LASHK^UK^AM'A ON INDIVIDUAL EOGS FED^ THREE MONTHS Con trol 6.64 15.9 43 11.5 78 78 0 0 22 0 0 0 0 0 6.40 15.6 41 16.0 75 74 1 0 23 0 2 0 0 0 Months on Tes t 1 2 4.20 14.8 40 9.2 65 65 0 0 33 1 1 0 0 0 6,76 16.6 43 12.6 70 70 0 0 28 1 1 0 0 0 3 6.52 16.0 43 11.0 77 75 2 0 22 1 0 0 0 0 104 39 170 2.0 9 6.7 0.76 - 0.1 2.9 100 L,A,C1 1950 N N N 6.4 0.1 N N 184 0 0 0-1 2+ 0 170 37 150 5.2 9 6.2 1.11 1.1 0.1 3.3 116 23 180 10.7 19 5.6 1.03 0.6 0.2 2.8 112 23 215 10.7 11 5.6 0.87 0.7 0.2 2.6 89 26 215 11.2 13 6.4 0.89 0.8 0.7 3.0 90 A,C1 2525 P N N 6.4 0.1 N N 184 0 0 0-1 3+ 0 130 D,A,C1,P 2460 N 1+ - N 6.6 1.0 N Tr 130 0 0-1 0-1 1+ 0 195 D,Y,C1,P 1546 N N N 7.0 1.0 N Tr 112 0 0-1 0 2+ 0 165 L,A,C1,P 2281 N Tr N 6.4 1.0 P Tr 148 0 0-1 0-4 2+ 0 Company Sanitized. Does not contain TSCA CBI Dog No.: Sex: Group: 1136 Female 750 ppm Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. My el. Lymphocytes Eosiriophils Monocytes Basophils Atypicals Nucleated RBC Biochemistry Glucose Urea Nitrogen Cholesterol Alk. Phos. GPT Total Protein A/G , Creatinine Bilirubin Albumin Urinalysis Volume Appearance Osmolality Occult Blood Sugar Acetone pH Urobilinogen Bilirubin Protein Creatinine MICROSCOPIC RBC's WBC's Epithelial Bacteria Cast HBH^OR i-'j-iiIIH-AI, l^yHJMIORYJ)ATA ON INDIVIDUAL DC)GS FEDj THREE MONTHS Cont:rol . 6.40 17.4 46 17.3 68 68 0 0 27 5 0 0 0 0 6.64 17.7 46 14.0 64 61 3 0 24 10 2 0 0 0 102 12 120 4.8 5 6.4 1.24 0.5 0.1 3.5 275 D,Y,C1,P 1677 N N N 7.0 0.1 N Tr 130 0 0 0-1 1+ 0 90 12 140 4.7 2 5.9 1.50 0.7 0.2 3.5 185 D,Y,C1,P 1388 N NN 8.2 1.0 N Tr 110 0 0 0-1 2+ 0 f fonths on Tes1L. 1 2 7.04 17.4 48 16.9 71 68 3 0 21 8 0 0 0 0 6.48 16.2 42 14.8 63 62 1 0 26 11 0 0 0 0 3 6.88 17.3 46 10.5 49 49 0 0 33 18 0 0 0 0 94 21 210 6.3 8 6.3 0.78 0.6 0.4 2.8 106 -- 12 275 7.6 9 6.6 0.98 0.6 0.1 3.3 109 12 235 7.9 7 6.9 0.63 0.6 0.2 2.7 195 A,C1,P 1772 N Tr N , 7.0 1.0 N Tr 114 0 0-1 0-1 1+ 0 135 D,Y,C1,P 1609 N Tr N 8.2 1.0 P Tr 132 0 0 0-2 1+ 0 150 D,Y,C1,P 1735 N Tr N 7.2 1.0 P Tr 242 0 1-5 0-3 3+ 0 Company Sanitized. Does not contain TSCA CB( Dog No.: Sex: Group: 1139 Female 750 ppm Hematology Erythrocytes Hmoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. Myel. Lymp.hocytes Eosinophils Monocytes Basophils Atypicals Nucleated RBC Biochemistry Glucose Urea Nitrogen Cholesterol Alk. Phos. GPT Total Protein A/G Creafcinine Bilirubin Albumin Urinalysis Volume Appearance Osmolality Occult Blood Sugar Acetone pH Urobilinogen Bilirubin Protein Creatinine MICROSCOPIC RBC's WBC's Epithelial Bacteria Cast CLINICAL IAB(3RATORY.J3ATA 0 N INDIVIDUAL DOGS ^^^"s FEDj--------B'OR I HEEE MONTHS Conf:rol 6.08 16.7 43 12.7 66 66 0 0 29 2 3 0 0 0 6.24 17.4 45 11.8 70 68 2 0 27 1 2 0 0 0 Moniths on Test 1 2 4.40 16.5 44 11.5 71 71 0 0 24 5 0 0 0 0. 7.28 18.5 48 16.6 79 78 1 0 21 0 0 0 0 0 3 6.64 17.1 45 10.8 62 62 0 0 30 5 3 0 0 0 104 19 170 6.1 7 6.2 0.97 0.5 0.1 3.1 160 Y,C1,P 1399 N N N 7.0 1.0 N Tr 112 0 0-2 0-1 2+ 0 110 13 170 5.9 3 6.2 1.00 0.6 0.1 3.1 98 - 21 190 9.6 12 5.8 1.35 0.6 0.3 3.3 120 14 250 10.1 13 6.4 1.16 0.7 0.1 3.4 99 15 340 9.3 9 6.8 0.92 0.6 0.1 3.3 80 Y,C1,P 1210 N N N 7.0 0.1 N Tr 128 0 0-2 0 2+ 0 90 Y,C1 . 1535 N Tr N 7.0 1.0 P Tr 94 75 Y,C1,P 1493 P N N 7.0 0.1 P Tr 172 90 Y,C1 1562 N N N 7.2 1.0 P Tr 158 0 1-6 0 0-1 0-2 0-2 0-1 0-1 0-4 1+ 24- 2+ 0 0 0 Company Sanitized. Does not (&otita!n TSCACBJ Dog No.: Sex: Group: 1144 Female 750 ppm Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. Myel. Lymphocytes Eosinophils Monocytes Basophils Atypicals Nucleated RBC Biochemistry Glucose Urea Nitrogen Cholesterol Alk. Phos. GPT Total Protein A/G Creafcinine Bilirubin Albumin Urinalysis Volume Appearance Osmolality Occult Blood SugarAcetone PH Urobilinogen Bilirubin Protein Creatinine MICROSCOPIC RBC's WBC's Epithelial Bacteria Cast CLINICAL LABiQEATOR^jATA ON INDIVIDUAL D0(3S FEDBIHllpOR THREE MONTHS Con trol 6.40 15.0 38 12.4 80 77 3 0 17 2 1 0 0 0 6.80 17.3 45 11.0 72 71 1 0 25 3 0 0 0 0 112 18 160 4.0 7 5.9 1,31 0.6 0.2 3.3 150 D,Y,C1,P 1898 N N N 7.0 1.0 P Tr 184 0 0 0 2+ 0 115 19 185 3.5 4 6.1 1.32 0.8 0.2 3.5 150 A,C1,P 1877 N Tr N 7.0 1.0 N Tr 188 0 0 0 2+ 0 Memths on Test 1 2 7.32 18.2 50 11.4 79 79 0 0 20 1 0 0 0 0 7.76 19.2 50 13.4 77 75 2 0 22 1 0 0 0 0 3 7.12 18.1 48 12.5 70 67 3 0 29 1 0 0 0 0 80 25 200 4.4 8 6.1 1.25 0.7 0.6 3.4 135 A,C1 1766 N 14N 6.6 1.0 P Tr 126 0 0 0-1 3+ 0 104 27 245 6.4 9 6.6 1.10 0.9 0.7 3.5 100 26 285 8.6 9 6.5 1.13 0.8 1.2 3.4 145 A,C1 1898 N N N 6.6 1.0 P N 148 0 0 0 140 195 A,G1,P 2194 N N N 6.8 1.0 P Tr 166 0 0-1 0-3 240 companysanw^ ^ ^^rsa^m Dog No. ; Sex: Group: 1148 Female 750 ppm Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. My el. Lymphpcytes Eosinophils Monocytes Basophils Atypicals Nucleated EBC Biochemistry Glucose Urea Nitrogen Cholesterol Alk. Phos. GPT Total Protein A/G Creatinine Bilirubin Albumin Urinalysis Volume Appearance Osmolalifcy Occult Blood Sugar Acetone pH Urobilinogen Bilirubin Protein Creatinine MICROSCOPIC RBC's WBC's Epithelial Bacteria Cast WATA CLINICAL IABO FEEf FOR 0IN INDIVIDUAL T'HREE MONTHS D0(?S Cont rol 6.28 15.8 42 9.2 59 58 1 0 41 0 0 0 0 0 6.88 16.5 43 11.8 71 70 1 0 29 0 0 0 0 0 Me)nths on Tes t 1 2 7.08 17.0 47 12.9 56 56 0 0 34 8 2 0 0 0 7.28 17.7 46 14.4 51 50 1 0 38 9 2 0 0 0 3 7.16 17.3 46 11.2 67 66 1 0 28 5 0 0 0 0 106 26 150 3.1 8 5.8 1.11 - 0.2 3.1 115 D,Y,C1,P 1777 N N N 6.8 1.0 P Tr 174 0 0-1 0-1 2+ 0 116 25 160 2.9 5 5.9 1.10 0,8 0.4 3.1 108 26 235 4.4 12 6.2 1.24 0.7 0.3 3.4 110 D,Y,C1 1856 N N N 6.8 1.0 P N 184 0 0 0 1+ 0 180 D,Y,C1 1436 N N N 7.0 1.0 P Tr 114 0 0 0 3+ 0 120 22 260 4.6 13 6.3 1.03 0.8 0.3 3.2 112 31 310 3.4 11 7.0 0.94 0.8 0.4 3.4 33 Y,C1 392 P N N 7.0 0.1 P Tr 50 0 0-1 0-2 1+ 0 125 D,Y,C1 2034 N Tr N 7.0 1.0 P Tr 208 0 0-1 0-2 2+ 0 Company Sanitized. Does not contain TSCA CB6 Dog No. : Sex: Group: 1134 Female 2500 ppm Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. ' Myel. Lymphocytes Eosinophils Monocytes Basophils Atypicals Nucleated RBC c i i i M l U A L F WLAB( EDf . T AOR ON INDIVIDUAL THREE MONTHS DC)GS Cont:rol 5.68 17.6 44 10.5 57 57 0 0 38 3 2 0 0 0 6.64 18.1 46 9.0 65 65 0 0 30 3 2 0 0 0 r lonths on Test l 2 6.40 16.8 45 9.0 67 66 1 0 27 3 3 0 0 0 6.60 17.1 44 13.2 75 73 2 0 19 4 2 0 0 0 3 6.60 16.8 44 9.1 68 68 0 0 29 3 0 0 0 0 Biochemistry Glucose Urea Nitrogen Cholesterol Alk. Phos. GPT Total Protein A/G Creatinine Bilirubin Albumin 112 11 140 4.4 8 6.3 1.37 0.5 0.1 3.6 100 9 155 6.8 3 6.2 1.15 0.6 0.2 3.3 91 13 235 15.4 14 6.5 0.94 0.6 0.3 3.1 110 16 290 17.7 24 6.7 0.97 0.8 0.2 3.3 124 17 285 15.2 17 6.8 1.05 0.8 0.5 3.5 Urinalysis Volume Appearance Osmolality Occult Blood Sugar Acetone pH Urobilinogen Bilirubin Protein Creatinine MICROSCOPIC RBC's WBC's Epithelial I Bacteria Cast 120 D,Y,C1,P 2116 N Tr N 7.0 1.0 N Tr 156 0 0-1 0 2+ 0 130 D,Y,C1,P 1798 N Tr N 7.0 1.0 N Tr 124 0 0 0-1 1+ 0 165 A,C1,P 1724 N Tr ' N 6.8 1.0 N N 116 0 0-1 0 3+ 0 175 D,Y,C1,P 1436 N N N 7.0 1.0 P N 100 0 0 0 3+ 0 170 A,C1,P 1903 N N N 7.2 1.0 P 1+ 144 0 0 0-1 3+ 0 Company Sanitized. Does not contain TSCA CB! Dog No.: Sex: Group: 1138 Female 2500 ppm Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. Myel. Lymphocytes Eosinophils Monocytes Basophils Atypicals Nucleated RBC ^j-ij.lIlUA W Fi, E1AI^MBS T AOR ON INDIVIDUAL THREE MONTHS D0(5S Cont]rol , 6.52 17.1 44 8.5 73 72 1 0 22 3 2 0 0 0 6.68 17.4 45 7.7 69 69 0 0 20 8 3 0 0 0 Meinths on Test 1 2 5.84 14.9 39 10.7 70 70 0 0 28 2 0 0 0 0 5.16 12.8 31 9.7 77 75 2 0 23 0 0 0 0 0 3 6.16 15.3 41 7.4 63 60 3 0 34 1 2 0 0 0 Biochemistry Glucose Urea Nitrogen Cholesterol Alk. Phos. GPT Total Protein A/G . Creatinine Bilirubin Albumin Urinalysis Vo lume Appearance Osmolality Occult Blood Sugar Acetone pH Urobilinogen Bilirubin Protein Creatinine MICROSCOPIC RBC's WBC' s Epithelial Bacteria /"" Cast 104 14 175 8.0 6 6.5 1.09 0.4 0.1 3.4 270 Y,C1,P 1399 N N N 7.0 0.1 N Tr 104 0 0-1 0-1 2+ 0 102 11 180 7.6 3 6.3 0.97 0.6 0.2 3.1 380 Y,C1,P 931 N - N N 7.0 0.1 N N 72 0 0-1 0-1 4+ 0 90 21 348 12.8 11 6.6 0.88 - 0.6 0.3 3.1 415 Y,C1 684 N N N 6.8 0.1 N N 48 0 0 0-1 340 120 -- 17 436 14.1 16 6.7 0.78 0.7 0.2 2.9 108 16 275 13.5 17 6.3 1.28 0.8 0.2 3.5 290 Y,C1,P 862 N N N 7.0 1.0 P N 82 0 0-1 0 4+ 0 175 A,C1,P 1420 N N N 7.2 1.0 N Tr 158 0 0 0-1 2+ 0 Company Sanitized, Does not contain TSC& Ca t=i hjpyo C^ lWW&.'.^.MCgs^nmh'-Oci-)t'B--'idO-aiO'oj?o(P>c0c0soo0oco3gp^>^0 <o1!-' i-'. p (U tUfu'T3t33w?dp)rti-'-CT' rl- (u C 0 (t C i-' crTn(rOntrFit-T-'--MOnMoTOio-y-r.Tai-fc-trf^f'itH--'*-. 0 i-! (- t-' PI j3 < 9 rt p) i-i (D tn (D h-'fa H. '-< (T) 0 !-' !-' 9 td !-' !3 CO H.l-i fUl-1- MtD OQ iO-i'c^tiOff p 0 (D 0 1-' P 04 W p>wn!i>HO!i>c'>ciO o' it-7''il--''.(nlO-^rTo>i-T3ipi-?'pO'nH>hc-!' o pr" fBiHi--fpr)t C pi 1-' (u o m >-' (D 0 y V S; !-" H- en oa h'- v ya'p H- h'- >-{ c0 r'(rDtHrt.-fD 01 ft i-l -O-{ (I-I)'- i-' o(q1 9 9 1-* tl s 1 t) h-f 2; > W 5; M t-1 !3 C rr pi o o '<; (I) r> o ^ M 0 w g t-1 w 0 rt i-'-d o 0 !-' <! (B H. n 0 V^ n- > (t rt OT ff 0 0 ^ 0 (" o y"~< o rt Co H- ft V 0 0 I-' 0 rr ^ 13 (i> H . y^f 1 (D i-' 0 (n 2 i-' n> in 01 H' rt t-' (B oi in EJ' 01 (I- !-' 1-' 0 01--000 0 U 1-' K! l--i >J 1\3 o cr> c") 4^- OOSiiSiO^gZSlCT'l-'O t-'oo '-d >- t-i P- M i-' 1*)00>-'CT<OOLJO'^>0 4>CT\(i-y' .i-'^ui (jj M 4> ^~ OOOOO'.WOl-'Ot-' I-' f~ <T< l-- I-' 0 Oh-'l 00 t-' v M ?^ l--' ho ' i--' 4^^l>--3iai-'O^aiHi-3g-M^Ci-'i'-U^il 000 l-d >-' I--' LntsatM L^OOl-'CTiWW'-n.OO LOhOOOOC^OO M M CT' a^ OOOO-^OOOOOl'-" i-' p M i-- w 1 V so 0010 ^ OMI 00 tff M M 'fs- >--' 1-' " 1--' (TPilS-3I-!!2!l---J5!tl--3^caor>o0l-t-t1n ^d . M bJOOl-'O^^t-Ci<^TUitisb3o'Mi i--'M'^lIsOa l--'M1 I-' w Ln (-n OOOOO^OOMM4> 0 >-' ( (jk> 3. N" D ^^ Ml-" 0. ho > 0 0 so rononnt-caaiTinni'nnaafi--peraI t-' Lo" h-- uiOtyi with Bacteria00 '^Si-'tr-'ziaissi'-ni-'vi ijoooocTiohi^-o-(oro>oMoOo M o^ cr' h-' oooo~~i-~joocri<Ti 4> 0 w 3 0 OCT. 'Xl I-'UIOO^O0 OCT'M ~-j fl 0 S3" 5' 1^0 t> ^-j (--1 w t--l NJ [-- i-iwi-oo - ON)000 itfl ~O-iOi-3H!s!H-'~~12;SSZ03(T1i-n'>c-nn MOl-'OiT.I-nooUll-'M h-' ho CT' 0' OOOl-'WUiOOh-'l-' oo 0 S'r '"d ^ONlOOOLOtTi 00 0^ 0 Dog No. : Sex: Group: 1146 Female 2500 ppm Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. Myel. Lymphocytes Ebsinophils Monocytes Basophils Atypicals Nucleated RBC Biochemistry Glucose Urea Nitrogen Cholesterol Alk. Phos. GPT Total Protein A/G Greatinine Bilirubin Albumin Urinalysis Volume Appearance Osmolality Occult Blood Sugar Acetone pH Urobilinogen Bilirubin Protein Creatinine MICROSCOPIC RBC'S WBC's Epithelial Bacteria Cast W..LH. Uil J-HU1V1UUAL UUGy FOR THREE MONTHS Cont]:ol 6.48 15.4 40 11.1 67 66 1 0 31 0 2 0 0 0 6.72 16.7 44 8.6 64 64 0 0 35 1 0 0 0 0 97 18 155 3.8 6 5.8 1.17 0.6 0.1 3.1 200 Y,C1,P 1189 N N N 7.2 0.1 N Tr 108 0 0 0-1 2+ 0 108 15 175 4.0 3 6.0 1.41 0.7 0.1 3.5 110 Y,C1 743 N N N 7.0 0.1 N Tr 136 0 0-2 0-1 3+ 0 V.[onths on Tes t 1 2 5.88 14.3 38 11.4 61 61 0 0 31 5 3 0 0 0 6.00 14.2 36 12.3 65 65 0 0 34 0 1 0 0 0 3 5.88 15.0 39 12.6 62 62 0 0 30 6 2 0 0 0 110 26 260 7.8 11 6.3 1.07 0.6 0.2 3.3 116 23 270 8.3 17 6.2 1.06 0.8 0.1 3.2 112 19 230 8.9 12 6.6 1.28 0.9 0.1 3.7 140 Y,C1,P 1247 N N N 7.0 0.1 N Tr 96 0 0-1 0-1 4+ 0 80 D,Y,C1 1724 N N N 7.2 1.0 N Tr 158 0 0 0-2 2+ 0 95 D,A,C1,P 1942 N Tr N 8.0 1.0 P 1+ 198 0 0 0-1 2+ 0 (company Sanitized. Does not contain TSCA ;Bl