Document mB0egqNraRKv5wqYwR3NNdOL0

ACUTE TOXICITY TO AQUATIC INVERTEBRATES (E.G. DAPHNIA) TEST SUBSTANCE Identity: P2e6r,folruoFrXo-o1c0t0an1o.ic(aOccitda;nomiacyacailds,opbeentraedfeecrarfeldutooroa-s,PCFAOSA,#F3C3-567-1) Remarks: The is a white powder. 3M production lot The test sample number was 269. is FC-26 referred The to by test the substance test laboratory as N2803-3. characterized, although The purity of the sample was current information indicates not it is sufficiently a mixture of 96.5 - 100% test compounds. substance and = 0 - 3.5% Ce, C7, and Co perfiuoro homologue wReermeartoksdifsiseolldv:e tThheetsesatmmpalteerpiraelpairnaati5o0n:5d0irweacttieornissgoipvreonpatonotlhesollaubtoiroant.orIyn the with protocol amendment, it isopropanol in a 50:50 was stated that the ratioprior to use. sample was combined METHOD Method: U.S. EPA-TSCA Guideline 797.1300 Test type: Static acute GLP: Yes Year study conducted: 1995 Year study completed: 1996. Species: Daphnia magna Analytical monitoring: Nominal and temperature were monitored concentrations. daily. DO, conductivity, pH, Exposure period: 48-hours Sprtoabtiitstaincaallysmiest,hmoodvsi:ngLCasv)eraangdeEmCest)hvoadluoersbicnaolcmuilaaltepdr,obwabhielintypowsistihblneo,n-by Tliensetardianpthernpiodlastioounrucsei:ngOtbhteaicnoemdpuftreorm scouflttuwraersemoafiCn.tEa.inSetdepbhyaTn..R. Wilbury Aquatic Laboratories Inc, Marblehead, MA from Research Organisms, Hampton, NH. an original culture from Test daphnid age at study initiation: < 24-hours Test conditions D1i6l0utmigo/nLwaatseCr:aCFOillteLred, deionized water adjusted to a hardness of D1iloufttihoen fwianatlerrepcohretm.istry: Representative sample given in Table Temperature: 19.4 202C 03933 LPihgohttoipnegr:ioCdooofl-1w6h-ihtoeurflsuloirgehts,ce8n-thobuurlsbsdawirtkhwaitnhiant1en5simtiynouft5e8 fi-c. transition period. Ssttoocckksaolnudtitoens(t5s0o:l5u0tmiioxntsurpereopfatreasttisounb:stAan1c,e00a0ndmgi/sLopprroipmaanroly) wpraospoprrtieopnaarleldy diinlduitleudtiwointhwadtielru.tioAnftweartemirxtiongp,retphaerpertihmeartyessttock was cEoxnpcoensturraetivoensss.elNso: i3n0s0olmubLlegmlaatsesrbiaelakwearss ncoontteadindiurnign2g5t0hemtLesto.f test solution. The approximate Number of replicates: two depth of test solution was 9 cm. Number Number of of daphnids per replicate: ten concentrations: five plus a negative control Water chemistry during the study: Dissolv8e.7d-o8x.y8gemng/rLa(ncgoentr(o0l-e4x8pohsouurres)): Conduc8ti.v7i-t8y.r8amnggleL(0(-14,0800hmougr/sL)exposure conc.) 590 570 ~ -- 610 590 ymhos/cm pmhos/cm (control exposure) (1,000 mg/L exposure conc.) PH range (0 - 48 hours) 7.9-84 (control exposure) 7.5-8.0 (1,000 mg/L exposure conc.) Test te1m9p.e5r-a2t0u.r2erCan(gcoent(r0o4l e8xpohsouurrse)) Elemen1t9.b4as-is2:02morCtal(i1t,y00a0ndmgi/mLmoebixlpiozsautrieonconc.) RESULTS N(Toemsitneadlacsonac5e0n:t5r0atmiioxntsu:re Bolfatnekstcsonutbrsolt,an1c3e0,an20d0,is3o6p0ro,p6a0n0o,l).1,0T0h0emg/L concentration mg/L. of test substance in solution was 65, 110, 180, 300 and 500 Element value (as-tosted): 2244--hhoouurrLECCssoy==18,40000(7(46000---5>710,)00m0g)/Lm.g/L 4488--hhoouurr LECCseoy == 870200 ((760600 ---- 972800)) mmgg//LL. 48-hour NOEC = 360 mg/L. Element value solution): (based on concentration of test substance in 24-hour ECsp = 420 (370 -- 490) mg/L 03994 4248--hhoouurr ELCCsso) == 530600 ((330000 --->550000))mmgg//LL 48-hour 48-hour LCso = NOEC 400 (350 -- = 180 mg/L. 460) mg/L All element values based on nominal concentrations. A toxicity test conducted no mortality or sublethal previously effects. with isopropanol at 390 mg/L showed SitnatetrivsatliscawleErveaclaulactuiloant:edThweheLnCpsosasnidblEeCbsyo pvraolbuietsanaanldys9is5,%thceonmfoivdienngce tahveercaogmepumteetrhosodfotrwabrienoomfiCa.lEp.roSbtaebpihliatny.with non-linear interpolation using Epp Biological observations: mg/L treatments appeared Daphnids in healthy and ntohremcaolnttrhorl,outghheou1t30t,h2e0t0e,stawnidth3n6o0 mortality, immobility or overt clinical signs of toxicity. Cumulative percent mortality: Conca | 26hous Lowe [|0 w] Control response: satisfactory g CONCLo USIOn NS crygions. dTehteerasm-itneesdtetdo bteest8s0u0bmsgt/anLcweit4h8-aho9u5r%LcCosnffiodreDnacpehinnitearvmaalgonfa7w0a0--s 920 dmge/tLe.rmiTnheedatso-tbeest7e2d0tmesgt/sLuwbistthaanc9e54%8-choonufridEeCnscoefionrteDravpalhnoifa66m0ag--n7a80was cmogn/cLe.ntTrhateioans-(teNsOtEedC)tewstassu3b6s0tamngc/eL.48-Thhoeur48n-ohooubrseLrCvseodfoerffDeacpthnia dmeatgenramibnaesdetdo bone 4th0e0cmongc.entwritahtiaon9o5f%tecsotnsfuibdsetnacnecientienrvsaolluotfio3n50wa--s460 omfg/tLe.st sTuhbest4a8n-choeuirn EsColsuotifoonr wDaapshdneitaermmaignnead tboasbeed3o6n0 tmhge/cLownictehntara9t5io%n 033895 ccoonncfeindternacteioinnt(erNvOalEoCf)3f0o0r t--he50t0estmgs/uLb.stTahncee4i8n-hsooluurtinoon owbasser1v8e0dmegf/fLec.t Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 ba0 DAtTAAe0 QuUAAL0 ImTyY 0000000000 oRfeltieasbtilsiutbys:taKniciemicsocnhcreanntkriantgio3n.s Tinhethsettuedsyt lsaoclkustiaonnaslyatnidcaslammepalseupruermiteynits dniotscsrueffpiacnicenytlbyecthwaereacntetrhiezseda.mpAldediptrieopnaarllayt,iotnhedriereacptpioenasrgsitvoenbetoathe lteasbtorsaotlourtyioansn.d the proc-edure conducted by the laboratory to prepare the R0 REEFFEERRENNCCE0 ESS 0000 TMhAi,sastttuhdey wreaqsuecsotnodfucttheed3aMt CT.oRm.pWainlyb.ury Laboratories, Inc., Marblehead, OSTTHEReee Last changed: 5/25/00 eee 03296 a BEST COPY Auris 1BLE study Title omnis, Eoptnis mar Set Seetsvaets isan aber - pnas et [vp-- a Ligh x par : |So aE Snge ekg Cee fn 4 J ee sMe, Gi usdiE ugl RGoopeEiA g pR aL n. o iet ae Lingo STE ol LfENima laini eeednls v2S maS TBLogCe E 2SERA CR UE R E ag a Se H Sa ESE {de Ti e BERR sL agegR AiT n SS ay svihon Re Ee he Sie oC Cr 3997 BEST COPY Avan ABLE t 3 I. 0000LABORATORY PRACTICECOMPLIANCESTATENENT A C abortHions: "ToeRarnE(ErlaThe hee y Datore edprotect mnt] : - HOfeoari ooH =Sat a HR Siwt BoE ea anet Slee a dob a fCioeeon ie i RSE ahoRrEeEvnRRSRERES BO Eos SA SE gop iag A a $Bm yGAB I ae le 0L U nhn Ls es at ACraLBEE S ae GE eGNnleBN ernR E tei h DeeSeEa C 0 19 995 BEST COPY AVAILABLE PL. 4 rn penne BENS 20 = |< ! cortticastons ST5.rhoasmmph nites,h f1ri,.vShet ammeaenepwrie, hE |! l Srhrr nco SaecenR y pee L iB Frater 4nd Boil Soi Mette ee Shree manecanens A ; te mimo pmo So aams imass yRaE m PE fe G Snigngefac o SE EE z SRL a See pS SoeeeEL ha E dnunT G a E S GRA L B n ss l ) a e e Sopee ws BEIT ALOE AHH SCISERERR S LL fr E geRSD ai S gEE U RE al TE RedaEaA ThE RE BEST COPY AVATit ABLE : I. GoodLaboratory Practice Cospliance Statement T II. QualityAssurance Statement : III. Table of Contents =v. Iden of Tables and Figures : :: Poa i VII. Introduction " VIII. Methods and Materials niin x. Raterances . . Te:in; ` froin, ee Quality Data from Toxicitywa 5i : . i CEREAL Heie AR a e 7 L Laaimee cnn ih b Gib b eCe FToEamRRE L CA e TE 2 LE biSe ei G b REe Eh T o ne Sp Lage FoliT agE e ala dea ee EHR SeEoEfRead sori SPE TEN ee a (02000 ds J 3; Rl k coBBmreenet ehthisnsett erodft+eTceesiIngtoaiTn,on 2 J ; Rie2. E set pEten sms oech ecokt som te u Boies. Esarnin E ant stn tta s tu fem he VB ET e Beni y st ser ee * ] iarog Be aE tHerI iHon evn e, ivo efrreenng foiemoSenneot,,irvnn, 12 i AS dal SSI I (het mime SE ] } SER FEE M2 a 53 SL SSELLAllind Sa a |i 2 ERea CERSiR SaR yEAkh ige. h LN Caa lmaE n oa ae aE bit a M CAe EREE LSae a a B RaR e nay HN ay 03 Za ERE 04001 B SRAERI R E E R Ba I Big a Raa mina3l So od ee ug3& nddbaontrcoeiuiTonrharsgeigeiLssyetc,ue,StnheTiaicCaasionrniieaenltxseMFyuaesrTlphoooifnarnLbt1ye0s9tST,3uooc-npa3SuteaeevnttnroitennerewtpehiseneevCidiotaamepsmshm,anicdFi,fedevheSsraonesth oacscoamaautgoa nneepi, s(]f]5n SToorriE n, Roy ar soSly aemienseearrdraeescahioegsvse tahdpeeenteltorsd HTScoe6nEm5ce"uEnatTihrhiaeratneioeanSnsceee1ro3fu.i a0rte3eec0nad2tp, iu%oss,nstbovrraessaeTD ndhsceeoaiknbstboL eudrtitoae"mnsirueSdeahS soiiosctieovcna oemswteiottieidrcoS encsveeosmewsiiheta hheei2feidve Concanteacions dove an accepts Ti S SropcohpE unielsa1l3seh$oe3f15p828S8ae3r-i3aEPsEuELpOpEiiteSad wAbiynLcthoetS sopolneserL r bvaos cI Somobeed i LwiStEh ahnJ coetloSneascsiEontethiaesTiic2kn4esTHeForvwoieTsitOru0Rroe1ei2snciShnuy 2ht3e7rdisMaeturoceettssoecie soevnewrmonoarhseihncoeosrsnovooFyrmemiitreELntcaem1220d2% TContaiol uephnlids S hua 4. sveckss wet walshS . eioeeesy ot 5oo I $BcoEh0nR0cE1uSSnSotE,enxipts3oiEsao3uEne4z.Saehn(oscLfeees2domahyepdhTimoaoafinads43o80ntt5toHaemcoNnt2oeiHravEte3o-TCh3soe-rmsee3nemetyssridechacadiSeot4mnnrc'eea(nhnee2mcs4caethtciohsoeeoenrknome4Se5eh8deisvsmonedesnot?2hTea 5 00 i BIC on E Sele L Th mar nd ECoErt)anLsoanCVS oLf SEEnE ASLI WbTroE bempnR e oma e ES) gS Broro L eran Co EAREG R A E E L ERE e secon oth bri ve CRE eeR l ESR HERS nae ; B aiiaa I ER CaS ee 1 SBEloagweivacy scaay wasner beS nSSiTn R Bre ; Aa a tlie eg f Bilaethe cdl T Lone CREE eT EGAIR CE :i : CE 5 ERR aa SR Gh AER LEE 3 CE ramiie eagad id eta 43 do on 14002 BEST COPY AVAN 45LF 2 Hatertal Testeds wares . 5 EiER sponsor: I company. SE 3 : paii,n B- EE. SHoiEnsCconto ah ics ots PAbiIn eEsiTn tYs w-- arn 7, a sti acsarien, 15Lo a iFsOhaES EEa ET SE SARE iE Ga, i vi LH uh e aa S Le amibiatis BR e E Bl SR LoR de EEE R SCE ee Hz oS OaBaIEaC t l ade Ade Io iRE S Eam rna N gen EEL i hah : Sep oc ELE sioscr R ERe A LLd eneCLLi A Sa R SSconeILTdhReMI2sEtY Jsun TsmwneeSeNesSebyn-- rhepcBomUp,esHn,T e15hrvaelh,,nMT aanneosn,,me SSSaeso LnBBnTErrpTeDSrA ri,,mdeei, eA Betprewe eesetTSeeteleeFoL1nScERL ; FR aui re STheo,HeTeL6TLSES0e0nC3,o(5Sp .0.hwEhnyeySLamoetEsoocea otnntanmpT ieee ewar a511)1 ]| Jof r miemtan e,rnT t eeTlEiAs Ue LfA r5I L AsL 2t,e SoenetA SrroASenSeesnpcirotmpne,atsmScLueEIr,Te : E S SEA E IL EDPe LHerieeolnSaeE sddbli ] mph LidTi So mE LAT AE go S77 RBorttnATgvan`SdS tEonceT ed mata ESiiteeLd heA EYVWihtyaTTThaavoTdraatoe cie1s1 3, Rly: aerated and cont: nucusly sSaRETnaTO 3 i ee E i BEEe S e e passed throush a particle filter, ultraviolet. Ei eHT e EvENE Een n Sa lT ie a ng i ta sroteeWEeg RnE e Can D | anR al gon ay i masenvde i i a NLR ERAes Sate = SPSEepRAEa al g e aS Peaies Sh Ra N ee b fo E io enh aReR : ly cotpoa RWBI RIA hs I I Ga s Bald eSee SEI fhi otcaho,NEpSpay : a omrneS mSeHOSwtw i ielteeHmHE RenWEaRmy : Table 1. Chemical charscterization of a representative 3 He om we : :sien SE wGerm = 3BE # OHm BBE He be :i i oac E5e Zi: gir %2 2 : eee : i mols oe os I. 4 3 Er E srEs. in = a2h Yon 0 aan AB PFEFd elRpME. ET ow ianR. 4i BEG merenonen bwanlt GRdartoRgRlUw fn ik E Ba S a l i SSslE o Te U 2 ST ocemersvrs means Bono Sata viedo ormare 4ol Ei EE 4 TB feheale Po ETROm L R m ReNEl R aN Twaaem Ci RE Sra saab Ri ar Cade CE SR oy LRT EG a2 e BEST COPY AVA huit Je ersaTceTe hheeorOagraingilienmael,T Wca ultsurepNvaeist wMooobutanhiine ce,de.onae Nuey sth sesre e1s,ann t1s99d s4adferosmurAiqm oouyaccieaeh: ess | rS SeevetnecovL necinesaneSteeiioDnUSsI3asdana`nrs13hsCeedsLnFssroLbToSe3s0.enr1 eTt3oomftoyiremeeseSETeRbuCenSeaenivlneed a : novhoEFDSueSrrnAipnignahsceelsoiiucnkNIriemronnSsaenptESoeiideWeenLsvhoeekdTsShoeebcoeauiUaeotntedoSra4or Sro2ApTdyIieshaepinMreae,etrahoenyweosecfe | | EafRpeRLa StSiIrotaioed rL SeySaagR mardao Th Sn SeavoeltiosneioTni aost sSeoreenkiactoAitatoCionn EkLE id A state cramping cont vs conesad trom noma 23 50 35. Soph Le Haa nEd La 0k.0C5e0, l03a0ea p,o7r0u0e Sha8antde83v0esEmsutriThoansa Sn morals I IN sE ucvivad seared at 300 mpg H SetTheedn deBfinhitiveS RiltoxyicitySE teasat T varwL peyS rforsTsdosfTcrhoarmTWaugnmuee t30etefeo?R 11 1 | ; EFriTceires ofathe v.i. d Tavireree ntal Festectios amsiey phar A2 amEa EEA a T5E ScS eoncenntrE attiS onoSvsFreE SncoAonS sibmt1aa sncs hanrdsdiit ociaonpwen e eosf 3Sn ek0ihn4S edTe S s ih | | Ee eif P Broorte c rineo soss rnemen i usne tLSe ERECGa e 0Ea Rn e S e e oftenn e os laon= 3 ARE ee Te] Es Henin ; GE EESEe r U e Epa al Ei ERR ae | BP SH hpSwono a inicn ynstaye mabecterE : oolEET EeSe Seta) E EEL Ta a Raa 3%4 Aai dy CSa i ETRREEE Be Hse 38 bMerddos A BrEeo i B gt Ae A BESTCOPY avs sip . 3 ; : Ek:: ; % = ESEy Ta 2. sent stonRscRmaLrfoy he Sanetey tat wish H28033 and _-- saoonvcatiosnse of Ten 5 cemtemss . B<o2pnstoaemag1n0a Gu onTest Dmuerasteiosns Tan308homu3rshans estas ot sia vetons sm oth of une dias ra wate of expats mpticne sone pr mticner vases: 3 Fepecsace usps Mstoun oisseives cxrens ine rm ; roseparion Lie soenniers PIT 15580 emmention or Water : Guality NaususemB anter|iLlaoosvotmnee onda Cm oxeyseeLtn,R 2psua,tteeSsepe, apraeetoeere, | 0 reise a SaC y dE n Ian ma abse ahE r ER f dEeh s5RondsSaEa imeS i cS EnR GI Se E I meE R attanstE , reLnSt R E mDRS Hd R Sula SE ee : 4 e LTEIE S RRTASAN CER SVCA e AE ATION ha f S aid SEAROY Rn. bibacy,study mumbo 832m [HEA Reh Ano RN solmeme a SSa amlsass esshwsealh le WE das 3 ns BEE REE E Sh TEA Aa UA Ga SR ete ALE RR A a a 907 Ls is 5 3 in Leeiantnn b iki E jaRiEE any poise vce ntseisanel 12 spt aoe mony i SparrnitRydIAShTr hTmi,n Eeonresse ro ne CTnEt samen51tt6eotToeILtoAPh 1LLn0 T0 doetuhc.5pcceonceritooen,vweeinh +n1153mststuens : SS SS tempIeaty l TIEoeS ILoItTemran feoSe,soaneneo sftt,arSSeShtire, aenrne Ss"hitofueSreielidnts man tseel ae fern 2SE Ln pe Tl! 5 SSahapeptetrie"Sh58i1erA eSeaelS nm,dSetleleheSeoe,te0o7 nea end290ImfSru]e 2 Bis i samen, : & saaernie at TA, tn SSLiSnrpi2eenteyd BgEhNET Dnna , Sm LITmai mTen e ee h ETe e ond ginaa LE a ea C e E RCO hr ha a Rs pba R TEE R IONE Sh P| EELon miesty C wmer vierA Eno SEE rane 12 oi ha Soe a i Shae Ll AaEeE bt Ali Baie SOeNsAR ihs e pd g 95008 nd 2 i fS eeerdSo fEL arluSbFlehameaectniaslrthveatnoeteadrodSuecienSg ehyheencSnturheto, ascemiiloEopoeenrTtnb8reitn2ese:0ahnnas i e SpSintC emleea, ,nt I SaietmlolaseTCtRhLatoEeTtLhnameAva1torffmTooeha3disfSSeerac4eT,as3FBCaaeotVn5eSn2nen1i7rd5e5y0 ; FETy L ST hiva]L ert Bl hsteSe te,ee l ere in 34 14 44h Stn nd vs or nice seas co von SSLEEoLEmLTNemELe(C cSe LNohat TomTaTaoteiressSmaoifiddoaTeoiLncidcsrtoMnaosn ibef d Soeme e enerfSiBett)s S Eeot oauEeleseEH SetTT TLaRvR ioiSEn0 eiB nLn 3es rr,dOr Bi eae Lo BRgufeitisk n THER --ER a Seri Ne cous Sakari sa BLeaene o gleee E maAS he isi ee ec oh IRR SE Fase Senn dIlE GE minibay sus seer i ome gR pim eR s s RL R eB da SUE aE oniy 3 : SR UcyEe S SRE reTR : 04009 tn AA ERRAR TRLae r Zi iH k Tor EY a E TIR tafe he sete venta E ge m _-- Tr 'B be we fF wrow ide _-- Ze pbei i x E Sob kon Eo se F no :FOE oI daE s ee ee e i3 Pos okos ooo 2 * FoR 50 Lows : wo rrr oak FE CiEN ? : Glia bE SE Gnd Eene S pielee Sn R AE EREL, l ey osauldR RRR Co RL CER ETfeE =x v C ouiy aloR o BOL AR SE Re E BURGaR Sil SRE pe SRL, "PEN etka z SARE ad RR Spradiiy FRASER LaeLdSsR i ae cooddtahe SHhEiR A saeix oseordi4 TELS63 reCT ANB vb sree Bi clin 3 toe Bg BEST COPYAyan ABLE Tle eEtoAsaE tan ete dtc tmm he Sooty son i" s ? rry r romper | (97%) Rep. hr he ne he he he _-- i i hainRe 0 Besa 5 EEE ers HIe ASIsERRASi aUL EaEEm ol aid54 OERe Ce SH CR got RR SGERR ReSinT a cASR oaDttohseln T R R eIiER E G a E Fe y E: Told Se naaCAG ela a LE RR BESTCOPY AVAN 4.5 A temene iBs. sis la :x a5 Sh F i 1 LV i. Elan E earn FE esFy | ; bEaiin i _ AL iri cSehlaaElG EE REnA e S e OL S i pirin SLi er 1g | A 5 Rk 1 ECSE EEE a | see hE het eS | 4. SSm EhaEe E naHl E CREER 1 012 esERR E ic E E te oe n te smnr TE Exposure nen ota 5 percent Ctettet et oe srt nd bos me `calculator B Gf 4 hows Twn 0ee 920 mane rote 5 24 hours ner 0m 940 mg/n 740 to 970 mg/n 6to 0mart probit rosie Cleatbso te 23 cotati bn te un ste 5 "BF 4 tes Lm en tmeeEn dee wo ke wn ma rene HE ET woe VETERE wild ee Sn, Re ecruaemeiiny Dil eR ee Sfm as n o e e N eea Ban She Baase i wainher pin GR es Gl Te h n h3ke eET a iti ] Min aaa ila CE EL FERRI A na SL WE TehSlneRR l +aaSlsd EA e SEERBeTb Sheba ansibig ELE vo ERS oa no ASE rT SaC mii RSeE Bes COPY ayaBLE i3 L5E i x mermcns Ee Ey rie RST See ose ie SE Jen ve to ions rn NSN o I i en LER yr oie :2i Faber 5 gE ge BE oop 3 elma Hn AIEEE 3 oe GAT a LE EO : b R BrE aE in RRae CE Y RESeTin eeeen SO SR 538) 3 sro Go TPR A 0 en, Se CRE PEE Ci : SA ii LA (04014 STC0Py py, Chait fib 2 ar. 2 ab RR oF Jali ben 410) iE w4 r 5= 4 Oh Kens sf "4 : : thetMage <i iagg :: a ARIE a7 iriS S o 5ado accdEE rioLSA y ST R EIEE iE R gER RwE a y eeClaHad : iS=oSmhieshsi a TdEha a cpio Se er atA SA i. ESO iES siiei val ESTCOPYAvi 51, [EE wr tn ersend', EN . AR wsFRorocon, aves: SNESheweedii S1nE1A syTt eieee mtinned ve eSipityS earooA E teeT ReeRgan . PROTOCOJB3L DEoViItEAeTsIONrSE:mlaR,, IoSrTen gEonSuwaerv9h: nes soeeep 3 ; SEE CSlAER EREEE oe G Lu e raat Sr bade Baa an Seemed ana : 3% re Van ty RE Beabe BEa ERd E ) RE IE 0016 R CR ie ; ; A Bod # rep FTAeRt ES asih Appendix a1 xasER quaynachfae sinschrsmeme For REET en e Ba mlooooo EaRsSRE ie a a RtLean B Sram E vel T Loa SE He iSLaHEmEebnaLan sL is R h TRa o ARSER GE a Se tyeLeo004017 A HErbESERSLS S haaeA te ia ea ra UiL r s dEeUeEEE . Fos. SsinedooTlmT cesniI on, L conor Hai E_e reese : wh ee LRM x wdi PomonE momo i - noun Ban momo oma : Dono om oom 3 B14 rede Bi dSSgitanas colinnGaiagGEi aaEtliBi Baasnvie nesEE So DR Re Sea Laet e a kr jy ALE SR yg on01s : en "i = ---- Clown pips i h Comon (control) 1 =- oun mam 3 > : 3 we bn 3 : = hy : * oa H : Gono Pa Raonienn 2m02ons Ent mar Coo Laie dele 000 ifFEAL LAT Gok EE A A ii Ve isa aCki s S Ri E GC$ oib a ; TSAo % g an o none Si o Bh Hy i: Ea io Gi aGd ooda Ihoatae Re s cnA] ae 5 Eee ed a i ooE Ane nEee Slaiiaanai R ge E i n Ea EL a RL Eas to Ed Negi Ea C . a ooa l x 1 2 TAS SseTliRet oSveetlh icS osntin on, SeeEnsEiiera, ipn, ?nd4 y x reson emi LINE TEE aL Bf emo. e wo ww ow WEE Dissolved oxygen (ng/L) conductivity Gambossem t _-- SRE m nom gt 5 2 % FE EFAAR LEhE i: i RRA FEE gmf go e Go e SRE TOME ERE a ConA TEE Hs reba wWrey C aa Rw C EA aRl wo CRN a oyA 7 Es B : : : sid hi aR TRG pe EE a Serene elopcoin SEE OE R i aR si aioe Sig oOLER 04020 oni BEST COPY AVAII ABLE a ; . SCR ERwwe wwE R E8o0w FmOowR -- he Pou Tt-- on EWRaEMsPa Bpe he 0d eae 3 Iota: i REE io F: ordOeRoST S de Lne E e AIO ST xwhiir sty womber'eri2 SL BEC CEE CE RAS Poe fe 0s So py apgerrs Slap SRR minding00dee1 Sa EE ae