Document m3EgkVpGr5wLxVy9YpB4yMNO

AR226-2868 Copies to: E. 1. du Font de N e m o r s and Company Haskell Laboratory for Toxicology and Industrial Medicine HASKELL LABORATORY REPORT HO. 58-78 Material Tested -J Haskell Ho. 11,890 Sample Ready for Testing 12/2/77 3Other Codes j - Material Submitted bv "^Textile Fibers Department Seaford, Delaware 96-HOUR LC502 TO ULUEGILL SUKFISK Procedure: The test material, in original form, was introduced into all-glass exposure vessels and diluted with laboratory supply TOter fd^yleld tKedesiredcohcentrations in a 7.5 final volume. An identical jar containing only supply water was designated as a control. Ten bluegill sunfish. (Lepomis macrochirus; 3.0 cm standard length) were randomly placed into each test vessel. Mortality counts and observations were made every 24 hours for a total of 96 hours after initi ating exposure. Dead fish were removed at the time of discovery. Fish were not fed 48 hours prior to testing nor during exposure. Ho aeration was provided during the test, and temperature was maintained at 20 0.2C. Dissolved oxygen and pH measurements were taken at the beginning and end of exposure. Calculation of the 96-hour LC50 and confidence limits was by pzobit analysis (Finney, 1971).3 RESULTS: Definitive Test Test Concentration (Z v/v) 100 56 32 24 20 18 13.5 10 7.5 Control observed Mortality C%) 100 100 100 100 80 50 10 10 0 0 P H ____ Initial Final 9.5 9.5 9.4 9.3 9.1 9.0 9.0 8.8 8.7 8.2 8.7 7.6 8.6 7.5 8.3 6.9 8.3 7.2 6.5 6.6 Dissolved Oxygen (ppm) Initial Final 8.5 8.5 9.9 9.6 9.5 8.1 9.4 7.6 9.0 3.4 9.1 2.6 9.5 3.8 9.3 3.1 9.4 3.4 9.8 4.6 Observations Complete mortality was observed within one hour at the 100% test concentration. 96-hour LC50: 16.6% 95% Confidence Limits: 14.5% and 18.5% Dose-Response Slope: 9.1 95% Confidence Limits: 5.2 and 13.0 5- Company Sanitized. Does not contain T S C A CSE RESULTS Con't. At the concentrations studied, the initial pH of the test material ranged from 8.3 to 9.5. Since pH extremes beyond normal physiological levels can potentially cause or contribute to a toxicological response, a supplementary study was performed with test material neutralized to pH 7.0 by the addition of hydrochloric acid. Supplementary Test Test Concentration (% v/v) 100 56 32 24 10 Observed Mortality (%) 100 100 100 100 0 PH Initial Final 7.0 6.8 7.0 6.9 7.0 7.0 7.0 6.9 7.0 6.8 Dissolved Oxygen (ppm) Initial Final 8.5 8.2 8.7 8.2 9.1 7.9 9.0 4.5 9.0 3.8 Observations Complete mortality was observed within 3.5 hours at 100% test concentration. S.iry very 3-ow acute toxicity to bluegill sunfish under static u n a e r a t e d t e s ^ c o n d i t i o n ^ d u r S g a 9 o ^ h o u i ^ e x p b s u r ^ It's 96--hour LC50 is 16.6% with 95% confidence limits of 14.5% and 18.5%. The slope of the dose-response curve is 9.1 with 95% confidence limits of 5.2 and 13.0. The supplementary test using neutralized soluti is of the material demonstrated a 96-hour LC50 greater than 10% but less than 24%. A comparison of the LC50 values for the definitive and supplementary tests indicates that the pH of the original material did not appreciably contribute to the acute toxicity observed in the definitive test. Composition LC50 = The concentration which is lethal to 50% of a population of test organisms during the specified time period. Finney, D. J., Probit Analysis, 3rd ed., 1971. Cambridge University Press. Report by; /JG/je! Edward Smith, Technician EJS/cgs Date Issued: 2/10/78 Report Ko. 58-78 Approved by: - 2- Phfllip W. Schneider, Jr. Chief, Aquatic Toxicology Company Sanitized. Does noi contain TSCA CBB ; ' DOSE IS EXFHtSSED AS i SCALE OF DOSE IS TRANSFORMED TO LOG(IO) INPUT DATA DOSE 10C.0000 56.0000 32.0000 24.0000 2108..00000000 1130..05000000 7.5000 LOG DOSE 2.0000 1.7482 1.5051 1.3802 1.3010 1.2553 11..01030003 0.8751 ; .tf EXPOSED 10 M : O10.. i'! 111000*.. ! : 1io0.. IQ. responding ...1100.. 10 10 8 5. 1. 1. 0. RATE 0.9999 0.9999 0.9999 0.9999 0.8000 O.50CO 0.1000 00..01000C10 PR03IT 6.7191 8 .7 1 9 1 6.7191 8.7191 5.6407 4.997 3.7131 3.7131 1.2609 lONTROL SAMPLE S U E = 10. ;uaTflOL RESPONSES = iPORTANEOUS UESPOriSb 0._ RAlb .0.00oc heterogeneity factor = NUMbSR OF POINTS : DEGREES OF FREEDOM ! DEVIATE ! G NUMBER OF CXCLES : AVG X _____ AVG X AVG V SLOPE INTERCEPT ruT SQUARED POINT P= .01 p: p= ..1005 p= .20 P= .50 p= .to p= .90 P= .95 ps .99 DOSE 9.2192 10.9483 ,0000 9600 1535 LL _ 5 1 U UL = 13.0376 95 CONFIDENCE LIMITS LOWER . UPPER 5.6081 7.5227 8.7788 10.5420 U51B.1 18.3551 20.2029 21.7364 24.7601 ,3895 ,9567 ,8760 ,1658 K34A 29.3894 34.1915 45.7326 > yS \ Company Sanitized. Does not contain TSCA CBI ip rtPLOT OF THE MAXIMUM LIKELIHGQD LINE FOR THE MAXIMUM LIKLIHOOD EST1MATES ARE: SLOPEs 9.127^1 IHTERCEPTs -6.1310 .A ,, ,, ,, SPONTANEUUS RESPONSE RATE=C.00000 7.0 + 6.9 + 1 6.8 + 6.7 + 6.6 + '9 '9 ' 6.5 + 6.0 + 6.3 -* J 6.2 6.1 + 6.0 + ( 5.9 + 5.8 + . -* ^ *. 5.7 + { 5.6 + 5.5 9 . 5.0 + 1 5.3 + p 5.2 + . * t t 0R 5.1 + 5.0 + E 0.9 + j. ' :' * 9 I 0.6 + c T 0.7 + 0.6 + - 0.5 + c 0.0 M 9' _ : 9; ' 0.3 + ' 0.2 9. 0.1 * 0.0 + .e . 3.9 + r 3.8 3.7 + * .* 3.6 v! 3.5 3.0 + 9 ; 3.3 + 3.2 + 3.1 3.0 9 .. | 0.875 1.060 1,200 1.029 LOG DOSE 1.610 1.799 + ' + + T + 4* + + . + + + + + f 4 + + + + 1.983 Company Sarsihzed. Doss no! conta!?? TSSA OBI