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ADIETARYLCS0 STUprDoYsWITHTHEMALLARD WILDLIFE INTERSNMARTEIQOUNEASLTLTNDO., PU2R7O2J3ECT NO. 454-102
Series 8U5S0..EcEnovliorognimeantfaelcPtrostTeectsitonGAugiendceyli(nGeras) CPPSNumber 850.2200
FIFRASubdivisionB,Section 712 OECD Guideline205
AUTHORS: CSeoaunr't.nGe5ayl.laCgahseery JRaoymaBo.nBLdesvVearns Hoven
STUDY INITIATION: April 21,1999 STUDY COMPLETION: April 26,2000 AMENDED REPORT DATE: Mirch 23, 2008
SUBMITTTEDO
Enviro53n5M5mBeCnoutrhpaolrALavatebionorunaetory St Pu, Meson $5144
Wildlife International, Ltd.
E4a5s9o8n,CMoamrmyelracned D2r1i6v0e1 0) 822.8600 Page of72
AvENDED 70690
ee
WILDLIFE INTERNATIONAL, to
=
PROJECTNO.0: 4stdstlo2
GOODLABORATORY PRACTICE COMPLIANCE STATEMENT
SPONSOR: 3M Coporaion TILE: FOS: A Dietary LCSD Styvithbe Mallard WILDLIFE INTERNATIONAL LTD, PROJECT NO; 454102 STUDY COMPLETION: Apri 26,200 AMENDED REPORT DATE: March 23, 2004 5c"T0h3is. Bormann Foon Agen 40 study was conducted in compliance with GCoToRd LFaabrora1t6o5rysnPrdac5ti5c,e 17 Aug Standards as58% ORC published by PeLr5in5tc5iplae0sno8ftGnponodeMLAaTbloEor.watio5nrygNPereaxcxtcic,ee,pN(oOCkDeEa/tGoDn(9e2.) 3324,5E0,nvAirognmerntaMonFoogfruacpihoNno.B4u5c, aPari1s,3
rr -- E aves ----eE t T---- GLP compliance; however,the characterization CLE mires TAOSom sonprer tn was performed according to 3M Standard Operating
i =-- Apa, SE 953 An GeaS y 00 Cn etin Weu5l0 HE
STUDY DIRECTOR:
Life Bahn SE tL ---- `Sean P. Gallagher
SPONSOR'SREPRESENTATIVE
Ah oe0.Loch
ore sos Joy
aoe cr069L
WILDLIFE INTERNATIONAL, LTD 30
PROJECT NO: 454-102
QUALITY ASSURANCE STATEMENT
This study was examined for compliance with Good Laboratory Practice Standards as published by the US. Environmental Protection Agency, 40 CFR Part 160 & 792, 17 August 1989; OECD. PrinciplesofGood Laboratory Practice, (OCDE/GD{(92) 32, Environment Monograph No. 45, Paris, 1992); and Japan MAFF, 59 NohSan, Notification No. 3850, Agricultural Production Bureau, 10 August 1984. `The datesofall audits and inspections and the dates that any findings were reported to. the Study Director and Laboratory Management were as follows:
ACTIVITY
DATECONDUCTED
Matix Fortification, Test Substance Prep, Analytical Sampling April 22, 1999
Feed Consumption and Analytical Sampling April 27, 1999
NeLcirvoerpsWye,igBhltosodanCodllBeiclteion,
Collection
May 14,1999
`Analytical Data and
Draft Report
July 7,8, and 9, 1999
Biological Data and
Draft Report
Aug. 30,31, Sept. 1,199
Final Report
April 18,2000
AmReepnodretd Final
March 19,2004
DATEREPORTEDTO; STUDY DIRECTOR MANAGEMENT
April 22,1999 April 27,1999
April 23,1999 May4, 1999
May 14,1999 July 9, 1999 September 1,199 April 18, 2000 March 19,2008
May 21,1999 July 16,1999 Septembe3r, 1999 April 19,2000 March 19,2004
Susan .Coleman Susan L., Colernan `Senior Quality Assurance Representative.
DATE___ 3-33-04
AMENDED C5069
WILDLIFE INTERNATIONAL, Lo
a
AMENDED REPORTAPPROVAL
PROJECTNO. dsi-i02
SPONSOR: 3MCaporaion TITLE: PFOS: ADiaryLCS0 Studywith he Malet WILDLIFE INTERNATIONAL LTD. PROJECTNOL 454.102 MLAB REQUEST NO 12723
STUDY DIRECTOR:
A PEN
Ser Sean P.ilo, Gallagher vin Toxicology
lo
Date
CHEMISTRYPRINCIPALINVESTIGATOR:
7 1 ovayn, F-L
Semin Aaya Chim
Bae52908
REPORT APPROVED BY:
MCisatReanowfYieaWshoasgtOpeeertat!irons i DirCeVc h7eNmooin s
Bi2e3 tlaeh 2004. Be V4
Avene 0693
--e ----e ------------------------------
WILDLIFE INTERNATIONAL, 110 5.
PROJECET e NO: 454.1
TITLE PAGE
TAOFBCONLTENETS
coo
i.
conPag1e
Q(GUOAOLDITLYAABSOSRUARTAONRCYE PSRTAACTTEICMEENCTOMPLc IANCc E STA. TEMENT.....
Page 2 Pag3e
REPORT APPROVAL... TAOFBCONLTENETS...
Pig4 Page$
T0A ABPERs ECES eee SUMMARY... cern
Pog Pog7
INTRODUCTION... OBJECTIVE... cero
rages nen Page
MATEARNDIMEATHLODSS...
Ba
Da TuUnSsUiSoAnOaCtEeTeo n ------ s -- --I--
P8 ee
DATitBnreRpDai.iraion
.
FEp-- ------I-- eTatto5e
Anaya Method A A -------- o--n ---- ----Cree
GGAhrnsoiemsvaNtliCBoonOdsy..WeighiFsede Contmpion.. e CII ePagea14
StilAnaya
pi
CI ee
RESULTS...
Pi 14
DBMiootdrylWAieneiasgyhwstsdnCdiFneiucdlCGobnssuarmvpatioonns.
i.oi
nInI I
Peogs14 eels
Gross Ness
III
--
CONCLUSION...
Po 18
REFERENCES...
Pi 19
610691
e-- e --------------------------------
WILDLIFE INTERNATIONAL, LTD 6.
PRROOJEICETCT NO; 454-102
TAeOomFBiCnOaNLeTdENETS TAABND ALPPEENDISCES
TABLE 1: SCtoumduylwaititvhePMFOoS,rfarolmaiMatllyrd Acute DietaryIToxEicity Page20
TABLE 2: MSteuadnywBiotdhyPWFeOiSg.h fomMallrdAcute DietaoryiTocty Page21
TABLE: `MAecneDeissdtCronTsouxmipentyioSn o(gybiwrithdaFyE)OrSomaMallard
POET
TABLE: DGiraosrsyPaT thologicSaO ltOybWHsY PcROvfSosmiaMoalnlasrdeAciee vee F825
TABLES: MaClumlualradtAivcesMorDtiaeltiatryy(TEosxtiicmiattyedSCaumyuliatthive Dose, PRmOS.g./krg) cfrvoam. Pig 26
APPENDIXI: APPENDIXIE APPENDIIX APPENDIXIV: APPENDIVX:
APPENDIX VI:
APPENDIXVIE
APPENDIX VIL APPENDIIXX: APPENDIXX: APPENDIXEX:
CetiiofcAaBteS. Dit Formulation.
Ansytcl Methods ad Ress... DictProaIson. ccc `CAumcuelaDtiivseaMoTroalxictyhybySPeynfwoitmhPFMaOlSlard AIncdieviDdiueatlaBroydTyoxWiecihgyhsSd5)ywfiothmPMFaOlSlard FMeaoldlaCrodnAscumtptDioina(rbyiTtoxsiyci)tybSy PyenWfirtoPamFOS
CHA0RPG0E6.
Personnel Ivoiedi the Sic
RevisedSty SATII. ccc Report AGOTSD ccc
Page27 PAS
Page29 SY
Page 44
en P3045
...
Page
Paget
Pages
Page66 Paget
App 10695
WILDLIFE INTERNATIONAL, tT 7.
rr(OJECNTO0:: 45d4s-t102
SUMMARY SPONSOR: 3M Corporation TEST SUBSTANCE: PEOS `WILDLIFE INTERNATIONAL LTD, PROJECTNO. 454-102 STUDY: PROS: ADietary LCSO Study witheMtallhard
RESULTS!
"Thedietary LCS0 valueforMallads exposed to PROS was determined to be628ppma. witha 95%confintiervdaloef4n8pcpme..to 958pm. i. The slopeofthe concentrationresponse curve was 3.67 and the chi Squarevalue was 213. Thenomoralityconcentration was146ppma. Based upon reductions in body weight gain at the 73.2 ppm ai. test concentration, the no-observed-ffect concentration was 36.6 pm ..
TEST DATES:
Hatch April 13,1999 Accimation - April 14:2, 1999 Experimental Sart-April 22,1999 Experimental Termination-May 14, 1999
CNOONMCIENNATLRATTEISOTNS: ~ 0,6.1,18.3,36.6, 73.2, 146, 293,586, 1171ppm a. TESTANIMALS: Mallard (nasplatyriynchos) AGETEST ANIMALS: 10daysofage attest inition SOURCE TESTANIMALS: ~ PWOhiBtoixngI-WAings
H113aWanshiIonLgvi6o1n0e4St1.r35,12 STUDYCOMPLETION: April 26,2000 AMENDED REPORT DATE: March 23, 2004
7sSeereiA]ppelnodwinXgforcthyecroemvpilseetdinoonminal est concentrationsad els basedupontherealy of oe et. AMENDED
10G96
-_--
Te WE ILDLIFE INTO ERNATIOT NAe L, 1D e PROJECTNO: 4st-102en .
This study was conducted by WildIeNTIReOmDatUiCoTnIalONLid. Intemational Lid. toxicology facily in Exston, Maryland.
for 3M Corporation at the The inf portion of the
Wildlife test was
acnodndacceodpyroofmthAeprnia22,re1po9r9ta9orMfalyed1u4,nd1e9r99P.rojReacteNduamtbegnrc4r5a0t-e1d0a2t iWniardcheiveIsnelomcaatteodnolnLhice
Wiis Imation Ld. sie.
The objective Patrhynchos)
of his study administered
ws to cvalutOeBtJhEeCtTonIiVciEty of through th dit for fv days.
a est subsance An LCS0 val
to wil
the mallard (ras l be calulted, if
possbic
"The methods used in conductM ingAthiT s sE udyARaNI DMbaA EsTedL HuOpS DomSprocedures specified a the USS. 8En5v0i2r2o0a0men(t1a)l SPercottieocntio7n1-A2geonfctyheSeErinevsir8o5n0meEnctoallogPircoatleEtfifoenctsAgTeenscty'GsuiRdelgiinsetrsatOiPonPTGSuidNeulminbeesr, OPersgtainciidsemsAs(2s)c;sOmEenCsDGuGiudiedleilniense, 2F0I5,FRGAuidSeulbidnievifsioonTeEs,tiHnagzoafrdChEevmailucaatliso,n:AvWiaindDieetaarnyd TAoxqicuitiy TToexsici3t)y;Taensdsuwpiotnh AAvSiaTnMSpSeucainedsa"rd(6)EBST.87,"Sandard Praicfo Conducing Subacute Dicary
Test"STuhbsttasncseubstance was recived from 3M Corporstion on Octobe 29, 1998 ad was ssigned pWoiwldedredneiefriaeodnass:! FLCi.d.95;dLetoitfiNcaat2i1o7n.NuTmhbeerrep4o6r7t5eduppotnyreocfeihpet.eTthesbfsesatnscuebswtasnce98w.a9s%,wwhitih oafnaenxpailryastiiosnidicnof20dt8es.tsiuFbosltlaaonwciengpuerttytoefrmina0t.i4o9%n., tAhlelteestsmcaotnercieanlrwtiaonessnhaavleybzeed.naThdesrtedsutso sretfolreecdttuhnedpeurraimtbyirenetpcoorndtioetindontsh.e new CenificateofAnalysis (Appendix 1)'. The test substance was
"Th Wilde
imma standard ntermtional Li.
was received denification
fom 3M Corporation on um452b6 upeonrercip.
Jly The
2, 1998 and was nema sndard,
signed granular
eeFvseolsloprvlyse.othnyewcseomiepildncp estdgo,yhse sok toFym67a5Ac.PwPWsrhechXtapracecntoinzienedseosseceoocvrteanowebiehcGhLoPnsandhssdtte AMENDED 010597
-_--
WILDLIFE INTERNATIONAL, LTD
0.
RROIECTNO. nn
materi, Number:
was deni as; 27619.972. The
1H, 1H, 2H, 2H standard was stored
Perfluorooctane Sulfnic unde ambit conditions
Aci,
Chemical
Absict
TsTaherteGstrocounpssisted of geometric serieofight est concenraon and contol group. Thirty omfaltlhaered adutckelinnggsrwoeurpe.sBiidgsnwe0erehdescoondtrboylgwreoiugphatnednencmhaolsleanrdinddisucrcimiknawtleelriyeransosmiggwnsietdhi1n ccaacchh rberporoedsienngtepdenwseicgohnctliasnfforvpldauccekmleinntgstcoachc.ontOronl Daanyd tO,atfomlelnotwignrgouxpps.ermTheetabledssiwaerteehoSupseadnfsn eignuietsitoendwteer adsdietiofnoorfthe 1a0ddpitmoncalttecsotncceontnrcaetitotn.io.BirNdomrionmalhde seamre lcootnctehntrraeimoanisnevdeadfitntshki.s s4t3u)d.y wTehreed0i,et9a.r1y, c1o8n3,cn36t.i6,on73s.2w,e1r46,es2a93b,ls5h9dabnadsc1d17u1popnartksnpoewmtiolroicacdttivse sindninfeorma(tpiomn supplied bythe Sponsor
he cEoanctholgrgoruopupwarsecfedivehdeuanptpersotperidatfeede.sto cFoolnlotwoinlgdtiheet ffoirvef-idvaeydaeyxs.poDsueripnegritohde elxlpogsruoruepspewreiroed evuethnaniuznerdanteddlibvaesralwedigihtfaonr tsheee,daybso.dO,nanDdaybil8e,saamfpleosfctohlncceadmfeonrtanaanldysicso.ntTohlebeirmdssiwneirge ivnerdwweeigrhtedanbdassaulea,tiboonou,nlanDdabtil2e2 On Dy 22, hesebirdsweessrifced ad ls sampled or
DustiTohen
oftheTet primary phasesofthi
stand
tei
duoswee:
1. Acclimaton-9 days
2 Exposure ays.
3. Postexposue observation 3 or 17 days
TestABLirdmasllard inition of th
(nas ts.
plathynchos The birds were
) were 10 obsined
day from
of age and sppeard Whistling Wings nc,
to be in god Hanover, IL.
health st and were
HactdchedohnsAspteuldy13w,r1e99i9.mmaBtiudrse raanndgecdoilndweniogthbterdoimf9f6en10i2e0d4 bgyrasmexs.atAeltbniidtsiowne,reTfhreombitdhse
610598
_--_----
WILDLIFE INTERNATIONAL, LT -10-
fRamcTNC Sil
csalmiemabittecho, tpeenrceagairnegdnadndfapcheinloetyrpiocmaltlhyeidnadyisainfguihsahtacbhluenflronmttwiioldn
bids. of he
All et.
birds
were
Avimal Die
Throughout acclimation Wide Inemational Lids
and testing al test birds specifications (Appendix
were fed a I). Wate
game bind ation from the ow f
formulated to Easton public
naotfeorrmsuopfplayn,tiainodtifceemdedwiecraetipornovdiudreidngaadccilimtatuiondua rintgestaicncgl.imation and sing. The birds recived
DietPreparation (ode"TNhuemtbesetrsAuSbsZtOa0nTc)e.waAsllmdiixceadryditecsteyconncoentrhaetroantisonw. eeMiadnjugstweads do1n0e0w%itPhFOSHobbaasretdmuipxoenr h59eprmepaorit.ednptuhseiydoieftt.heNoemtinsaulbstdainccet.a Aeslt dciotnacreyntcroantcieonntruastdionnatnhdisthse yLCwSeDrvealue9.a1,r 18r3e,po3r6te,d 732,146,295, 586 and 1171 ppmi. (AppenV)d.
DictSampling
Samples confirm the
of he tet dicts were collected stably and homogeneity of the
0 veiy the test tet substance dn
concentrations administered and fo the dicts. Homogeneity ofthe es
ssuabmsptlaenscefrionm tthhee d1ie1t71wapspmev.a.lua3t.edebsty dcioltleacttipnrgepsairxatsiaomnpl0e0s Dfaoym0.thHeo9mo.gepnpemit4y.saim.pleasndwesex
aHolmolgetneiftrymsahmeplteosp,almsio dseervnedd1b5otvetroimfiocatfihoen sIafmplaensd.rigOhntesevetroinfiscaotfiotnhesammpilnegwvseeclo,llecTtheed
from the control die and two 810up at preparation on Day 0.
verfiaion samples wer At the cnofthe exposure
collected fom period (Day 5),
ach remaining one sample vas
restment collected
hfreomestthesucbosntiarnlceainndtwheodicsta.mpTlhees wseareiclollseacmtpeldesfowemreeaccohllrecetaemdefnrtomgreoeu oremdaeitneirnmgiinntshaehfeielderosf
aWfitelrdbeeiInngeamtaatmobnisentLied.tspneanytccolndcihoemnisstrfyo.r fv days. Samples were wnsfeed immedisiel to
AnilMethod deveTlhoepedmeatt hWodiudsedInftoermtthieonasnlalLyts.issnodf etnheteadvi"nMedtiheodsaOmuptlliense wfaorstbhasDeedteuempionnatmieotnhoofPdoOlSog,y
52699
_--
WILDLIFE INTERNATIONAL, to ne
ma
in Avian Fest, requisite quantity
Avian of eed
diet samples containedin a
were extracted with Frach-squae las
methanol. Methanol was bot. Bottswer capped
added o 3 nd shaken
voniandshtakherr atbilm.es Swaitmhplmeesthwaenroelviatcouutmheffirraetd.usiTnhgeqfuiaatlivaslerrnspafpeer.dT0h evtoalnumeedtrfiecdfwlaocek
Eanadchbrsoaumgphlte0thvnolwuamse dwiitlhtdmetwhiantoal.0A%s ampetphraonporli:,5s0a%mpNleAsNweOrre efurthweartderistoelutwiiotnhcmoentthaainnoiln,g
0l.i1b0r0amtigo4n HanPgFeOSof(tnheerPsR!OSstmaentdhaodrodl)og3y.nd
0A.0m5e%thfoodrmfilcowaechiadrt(ivsv)prosv0idtehdeyineAlppweniditn
the 1,
Figure 1.
perfCoornmcaenncteraltiiqounisd cofhrPoRmOsSogirnatphey sutsanidnagrdHs eanwdistaPmapclkeasrwdeMreoddeelte1r1m0i0neHdigbhy Preevcefrosrem-apnhcaesLeiqHuiigdh
EChlimrsomaTtuobgoralpohn(pHrPaLyC)iownistohuarPcee. iHnPELlCmerseApaPrIat1io0n0sLwCeMrassasScphieecvterdomuestiengrcaquKiepypsetdownietBhaetPaekCiny
analytical column (100 mm x 2 mm LD, 3 pum particle size). summarized in Appendix I, Table 1.
The nsrument parameters are
Calibration standards contining 0.100mg 4H
of PROS prepared in 50% PROS (meal standard)
methanol: $034 NANOpuze wate solution snd 005% formic acid (vv), ranging in
cpornocmeinnternattiponefsormesp0o.n0s2e2f9or0P0E.O0S457wams gutil8i.1zLewdetroemaanrailtyzrePdFwOiSthitnhalslacmapllebsa,tiToh,eqsuaalmietyancdonmtoslt,
and study samples. components. Linea
No aiempt was made to regression cquations were
quantity PROS on the gencrted using pesk
basis of individu area response ros
isomeric (PFO
cianltibrraaltiosntasnudnadradr)dsv,ersAustyhpeicalrescpaecltaitveiocnonccuernvteratiisonprersaetnetsed(PinFOASpp:enidnitxemaTlI,stFaingduarred)2. ofThhee
icnoonctheenaipoplnicoafblPeRlOiSnairntrehgeressasmipolnesequwaatsiond.eterRmepirneesdenbtyatsiuvbesiiounicnhgrothmeatpoegrsamasraofrelsopwonasedthiigsh
libration stanadr aprersedntsed in Appendie I, Figureansd , respectively
he pTrhoedumcettohfotdheiilotweosfqtucaadlitbarattiioonns(iLanOdQa)cdfarnatlhyezseedan(a0l.y0s0e2s29wamsgsea1)1.a1n5dptpe i.t.iconlfoer oa5f he mai blank samples 500)
C2700
-_--
WILDLIFE INTERNATIONAL, 10 2
PROJECT NOL 454-1"02
Two mtx eeobserved at
blank samples were ansyzed to determin or above the LOG during sample analyses
posible interiences. (Appendix I, Table 2).
No nerfrences An nererence
bineltohw fthee LapOp.eareAd orteparpepsreonxtiamtiavteelcyhrthoemastaomgerarmetofaianmtaetisbtlhapiessproefseinntteerdesitnbuAtppitenwdaisxweIl,l
Figures.
sampAvlieatnoddiettewramsinftrheimeedanatp4r5o7,cu1l83raencdove1r9y30(Appppmenadi.i Ian,dTaabnalley3z)e.d Scaomnpcluerecnotnyecinatthiotnhse
Wee no coreced fo he mean procedural recovery of 4.7%. mati fortification s presented in Appendia I, Figur6,
A rpresctative chromstogram ofa
183
An ppm
example calculation ai in via die.
i
presented
for
sample
number
454-1022,
nominal
concentration
of
ia Weigh 100 Fon Volume: 200ml PDiRlOuSioPneFsacArva:: 101013r5m68edste ion ctrx fl ion for) PinetkemAalvSaaRdata:t P0s27k4A9vs: 413160 Catbraion cue cquion. Sipe: 277297 np 001894 Curves weighed 14.
POS ait min Note: 15. ra str.
C (sk = rio ((eFmie)r1x1)55.CCoonsieinon
2169-0018x909100 mr.
-
27197
= oommmgain
n070L
WILDLIFE INTERNATIONAL, 10 is
PROJECTNNOO: 454.1dstioe
HOSrms mre = L PROS (mg/L)2 tne 0 xFFilna Vo8 ohlonse(1))xxD) lilsi Factor e oox0a m am 100
- 15spomai
Pra Conn F#70E5(Spm Rai m insample e
Tw --t x100= 101%
During acclimaion an testing, al controlled bronding pens manufactured
bids were housed indoors by Safeguard Produc, In.
in bates of Esch pen hd
thermosiaicaly floor sce that
lmeiansgursedansdpplrooxrismawteerley 6c2onsXtr9u0ctcedm.ofCeaiblianngzheedighsteweaswaippraonxdimsahtcetliyg2.5.5Bicrm.ds wEexrteernsaolrewdalbly,
pweenisg.ht,Estchehngcrhooupsnofinbdiridsscrwimaisnadteliy ffrombwyitpheinn ucamcbherreparensdenetted cwoengcchrtatciaons. foIr anssdignumenbtits
were identified by wing bands.
(SD)DurAivnegrtahgeeeasmtbhieentaverroaogmettmepmerpaetrces nfotrhtehisrsotundygwacsom2p5a2rtmCen4to0f7thCep(SeDn)s wwiatsh3a8avC+eragCe
relative midi Hours of igh per
of 33% + 18% (SD). The day during acclimation nd
photoperiod throughout
(naisined by th est. The gh
time clock) sure was
was sinteen fluorescent
aHpipgrhosxiwmhaitcehlycl2o0s7ellyuaopfplruoxmiimnaatteonno.ondosrysisnungliagnhtd, usThhaendbsiydpsrawcetirceescwxprseedbas1e0daonnagvueindgleineosf
stblibsyhthde Nationa Research Council 5.
ObseDruvraitnigonasclimation inj were noi ued.
al irc wee ahsrsd daily. B Following test nition and
ids exiiing n coniming il
ormal termina
behaovphiysoicral tion, al bis wre
00702
WILDLIFE INTERNATIONAL, 70 1.
nJEaCT nNO:an45-1
observeda least once daly. abnormal behavior.
A record was maintained of all martality, signs of toxicity, and
AnimalBodyWight/FesdConsumption bird,InadinvdidounalDabyosdy1w5eaingdhtDsawyer2e2 mfoerasaulrreedmaaintihnegibniirtdisa.tioAnvoefrtahgeetfesete,docnonDsauymp5t,ioonn vDaalyues dfuorrianlg tbhye epxepnosfuorrepeeacihodt(eDsamyesn0t-5g)raonudpthandpotsh-eccxopnotsroulr gobrsoeurpv.atiAodndipteiroinoadll(yD,ayfse6ed.8cownesruemdpettieornmiwnaesd ceotnesrummipnteidonfowraDsadyse5-t15eanrbdym1m5ie.2sn2sfuoerridtghethreemcahiannigneghianltfhoefwteheitgroefhatthtmenfteeadndprceosnetntoeldboirtdhs.e bFieredds ounvaevoaridgaibvleen wpaesritoadgoefotfiemee.d Tbyhethae cbicds.urofafceeyd consumption values mayhavebeen affecbyttehde
GrosAslNetcertobpisrdys that ied during the course ofthe tetand al birds rematnng a the terminationwere bsiulbejcwcetreed ctooalegcroesds fneocmropbisryd.s AedutdhiantioznedalonliDvearyw8eiagnhdt a2n2,d alinvderwthisesnue,possesirbulmeafndombltohoodsesotlhiadst,dainedd uring the courseofthe sud
StatisticalAnalyses
Mortalit
designed to
y data
calcul
wer
ate
e analyzed using
the LCSO valoe
the
and
computer
the 95%
programofC.E. Stephan (6).
confidence interval by probit
The program was
amalyss, moving
daveetreargmeinemdetbhyodthoerprtohbeitbimneotmhioadl. prTohbaebislliotpyemoeftthhoecdon(7c,en8t,ra9)t.ionInrehsipsonssetudcyurthveaLnCdSr0esuvlatlsuoefwtahse
TgoOoXdnSeTssATofsoffittwatreest(1a0r,e11)r.epoNrtoeds.atsBicoadlyawenigahtlwdeayrteasspwpeelreesdctoompfadrecdonbsyumDptuimoentdtts.test using
RESULTS
DietAAvniaalnysdiiset samples were collected rom the 9.1, 18.3 nd 1171 ppm a. est concentrations and
analyzed samples
to evaluste also served
homogeneity of the test substance in the avian as verification of test substance concentrons.
dit. The analysis of these Resuling mean measured
620703
_--mmm-s
WILDLIFE INTERNATIONAL, 10 "se
pointe
Wceornecen9t.8sti0on9s6,9stpanpdmar1d. de(vCiaVti=on9s.9a4n%d),coe1f9fi.ci5e%nts2.13ofppvamriaa.tio(nC(VCV=)1f0o.r9%th)easnedte1st c1o2nc97en06tr2atpiopnms h33e.t(eCtVs=ho5w.e8d7%n)o, irnetseprefcetrievnecleys(aAbpopveentdhiexLIO,Q.TaSbaim4p)l.esCoconltlreoclteadvidaunridnigettsheameptlestocvoelrliefcyttehdeda3r6i.n6,g 4730.22,,714456,,219734,a2n9d15a8n6dp53p7pmpm. taeit.sruebsspteactnicveelcy.oncTehensteiovnalsuehsadrempreeasnenmteedas1u0r,ed1c0o2,nc1en1t9r,a9t9i.o3nsanodf s9a1m.p6l%eosfctohlleecntoemdifnraolmcofneccednetrrsataifoenrs,beriensgpehcteidvealy a(mAbpipeenntditxem1p,erTaatbleefo5)r. vAenadlayyssisavoefraavgieadn 1d1i9e,t S9876.41an3d0,1117004,9p4o3m,1a0i.1,t1es0t1 saunbdst9a5nc0e%ocfonctehnetrDatio0ansv,alyrueesspecftoivteel9y.1(,App18e3n,di3x6.6I,,75T.a2,bl1e4)6., 295A, representative chromatogramof et sample i shown in Append I, Fire 7.
behaNvioormatrhtraoluigtheosutotchceutreset.dTihnetrheewecornetrnool tgrreoautpme,nta.nrdltseldbmirdas wrerte olnoortvmeaerltisniganpspoefatroaxnicceityanidn Whaes92.10,%16(.23o,f36.160,) 7m3o.r2a,loirty14i6ntphpem29a3.prcpa8mm.enttrgeraotmuepnst(gTraobulpe,13a0n%d (A3popfe1n0d)ixmVo)r.taHloitwyeivnetrh,eth5e8r6e Pom ai. treatment group and 90%(9 of 10 in the 1171pom a. restmnt group.
In Day.
the 293 ppm a. trcaument group singe Signsoftoictywerefirst observed on
mtohretamloirtnieisngwoefeDanyote4danodnctohnetminoumeidngunstiolfteDramyin7atainond
Dfoaryt2h2o.seAblilndbisrdssacirniftiicsedtroenatDmaenyt evaenlddiunstpillaytehde sflrinoofgotnonsiocfistDy.ayTh8esfeoritnhcolsuedebdirrdesdsuaccerdifriecaecdtioonn
to stimuli weakness.
(sAolulndreamnaidnimnogtiboinr,dslposesaorfecdoortodinhaatvieonr,ecuofvelrded
sppearance, lethargy, and were normal in
and lower appearance
limb and
behavior fom the morning ofDay until et termination.
on thAe mthaemi58n6gpopfmDaay. 5t,rDeaatyme6n,taendveDathye7r.e wSirgenstohfeettorxeicaittmyewnetreelaftisetdombsoerrtvaedlso,n othneemaocrhninnogteodf
Day Day
3 8
and for
continued unl terminationfo those bcs sacrificed on Day
those 22.
birds sacrificed All birds in this
on Day 8 an unl the afternoon testment level displayed signs
of of
toxicity. These included reduced reaction o stimuli (sound and motion), ruffed appearance, ower
Cno704
-_--
WILDLIFE INTERNATIONAL, LTD
16-
SROIOTNOS S02
limb weakness, appearance and
and lethargy. behavior from
All remaining birds the mornoifDnagy 9
appeared until fst
to have recovered termination
and
were
normal
in
nthe mortalities
171 ppm a. were found
treatmentgroup on the morning
thre were of Day 4,
nine treatment followed by
related morals. four mortalities on
The first Day S,
two. two.
tmohretmaloirtniiesngoonfDDaya62aynadndthtehefisnisn!glmeosrutravilviiotnngybDirad yc7o.ntOivneuretd soigdnisospfltaoyxsiciigtnyowfteriecifitrsyt otbhsreoruvgehdtohne
afternoon included
of Day reduced
8. Signs reaction
of toxicity observed among bids in to stimuli (sound and motion), loss
the 1171 ppm i. eatment group. of coordination, prostrate posture,
bdierpdreaspsipoena,recdontvoulhsaivoensr,ecrouvfefreeddaapnpdeswraasncn,olromwaerilniampbpweeaarkannecses,anadndbelheatvhiarogry.froTmhethoenemosmurivnigvinogf
Day 9 unil et termination,
weigWhtheanmocnogmpbairredds itno tthhee c9.o1n,tro18l.3g,roourp,36t.h6ereppwmeraei.no(arpapmareenntgtrroeuapst.mentThrerleatewdaesffactssaiosntcbloldyy
significant (p<0.05) eduction ia weight gan at reflected in significantly lower (p< 0.05) mean
the 9.1 Day 5
ppm body
a.. evel weight.
orth When
Day 0-5 period that was compared to the control
r8ersouulpt,inrgedinucatisoingsnifinicwaentilgyht(pg<a0i.n05w)elroewaelrsoDsataytistmiecaalnlybsoidgynifwieciagnhtt.(p<H0o.w0e5v)efro,r dtihfefeDraenyce5s-8fipoemritohde,
o9-n1tpoplmgraio.upleavtetlhaen9d.1weprpemnao.t. deovseelraepsppoenasriv(e0.beThdeureef1or3e,ltohweesremiefafneeDnacyes0wbeordeynwoetigchontsiodrertehde
reatment elated
73.2T,h1e4r6e,2w9a3s, 5a86m,arnkded,11t7r1eaptpmmenati.eltarteeadt,mecnotngcreonturpastidounrsiensptohneseixvpeoesfufreectpeorniobdo(dDyaywsei0g-h5)t (iTnabtlhee
e2vaenldaAndppaetnpd<i0x.0V1I).at tThhees14e6,re2d9u3c,ti5o8n6s awnedre11s7t1spipcmal.y. sliegvneilfs.icanDturaitnpg<t0h.e05Dafyor5t-h8ep7o3.s2tp-epxpmosuar.e
tpreeraitodm,enwtegirgohutpsg.ainThceosnetirneudeudcttioonbsewreerdeucseadifsocralsulryvisviignnigfibciarndts aitnpt<h0e.02193,for58t6hea2n9d31a1n7d1 5p8p6mppa m
oi. levels `compared.
Differences at the 1171 ppm .i. level were Body weightlosses an reductions in weight gain
marked but could atthe 293, 58 and
not 1171
be sutstcally ppm. levels
were reflected insignificantly (p<0.05 or p<0.01) lower mean body weighs on Days 5, 15, and 22
n0'705
-_--
WILDLIFE INTERNATIONAL, to n-
FROJECTNO: dss1o2
(OpF<t0h.e05e)s.lowAeLr thhean1t4h6epcopnmtraoil. glreovuepl.meDaunribnogdtyheweDiagyht-s1o5n aDnadysDayan1d5-2222pwoesrteeaxploosusriegnipfeirciaondtsl,y body weight gan appeared comparable amg all groups.
293,W5h8e6,nacnodmp1a17r1edptpomthae. ctonetsrmolengtrogurp.ouhpesrdeuwrains themaerxkpeadsurreedpuecrtiioodn (inDafyesed05c)o.nsuAmprteidouncitnitoihne
feed consumption reament groups.
continued
to
be
observed
through
Day
15
in
the
293,
586
and
1171
ppm
as.
GrosDsuNreicnrgotphseycouse resus for birds found
ofthe des
test, were
ll bids similar.
t
hat died were subjected Common observations
10a gros necropsy. Necropsy included thin condition, loss of
muscle mass, altered spleen color, consibdeetersmeendt aed.
Deetmapitlsyofctrhoep,neacnrdopesymfp igasntraordintpiersetsinneanlgteadcsitns.TabFliend4ings
were
DayH2,aoflfoltlfhowisnugrvteisvtitnebrimridnsawteiorneTshuebincetcrdo0psygrreossulntsecfroopbsyiarncDubaayn8izaendd tonhDraemyaiianngdbDiardys 2o2n
furopm haend18o.n3,e1b4i6rd3idn th1e17316.p6ppmpam.a.rerseesnetngtrogurposupwewreereunnroetmeadrkwaibtlhe.retOanineedbiydoliknstahcescuorntirnogl
necropsyofbirds on Wh OR 3 Smal,
Day ith
.
lAocksionfgemubsicdleimahses. 9.1Inptphe a7i3..2
repstpmmen.t.grroeupsenenctrogprsoiudpoanneDabyd
necropscd on Day was caceous ecrti teil
small with a on the igh
ck id
ofmuscle mass nd had a2 ofthe abdominal val. Due
0.5 cm abcess containing 0 he matureofthe sons
boibdsserfvreomintbhierd9s.,1,an3d66t,hanldat73e.d2
incidence ppm a.
rocfaomcecnutregnrcoeu,pshewefriendninogscolnssiddearbeodvteo
in cubnized be related to
ramen. In adion, al remaining rows wer no remarkable.
bids
in
the
control,
9.1,
366,
and
73.2
pom
a.
resent
findiInngthoefa29r3etpaimneday.olrkessstc,menA gsreocuopn,dobnierdinind tcheh2a9i3spepmona.Dagyro8upw,asneocrtoepdsiwcidtohnaDiyncid,enwtaasl wnoetiegdhatemgaaicnifaotretdhewiletvheal laancdkowfmaussccolnesimdaesrse.d tTchaitsmoesnst orfelmautesdc.leAmnaostshewrasbicodnsnistheent2w9it3hprpedmuced ramen group, cuhanized on Day 22, was noted with 2m cysts containing yellow id on (he
600706
EE E E----...
WILDLIFE INTERNATIONAL, to 1s
FROJETCNOTNO d4s53.102
oafntoecrciuorreonrcieo,niosfbthserevttioobn wofatshoetlicvoern.sidDeureedortehseennatturreeolfehe cysts and he solaed neidence nedThtroebbeirtdhsininahned 8la6ckipngmm.i.stcesmmaesn.t eOvneel wbeirdewualsbannoitseeddwointDhaayl8.gAhttnyecprloepsvyerheyTwheorsee sfuirndviinvgesd uarnclcDonasyid22e.redNetcorobpesytreesltrmsentorretlhaetesde. fouFrobuidbswiesrinnohtereSm8a6rapbplm. ai. eaten feet
`CONCLUSION
95%TchoendfiicdeanycLiCnSt0ravlaloufefo44r5Mpalpla.r.d e1x0p9o5s8eptomPRa3O.S wTahsedseltoepremoifngthteocboen62c8pepnmtar.eswpiotnhsa vBaseedwuapson3.r6e7dauncdiotnhse cin hboduyrwegvhatlugwinaast t2.e13. 7T3h.2e pnopmmor.aliltsyt ccoonncceenntiraaitoionnwhans 1o46pspe. tfc concentration was 36.6 ppm.
50707
_--
WILDLIFE INTERNATIONAL, LD
"19
PROJECTPtrNO: 454-
REFERENCES
1 dU.rSa.),EnOvPiProTnSmeNnutmablePrro$t5e0c2t0io0n:AAgveinacny,Die1t9a9r.y TSocvieey50To-t.Ecological feTesctGutilisn 2 ESUunSbv.diirvoEinnsmvieoinnrtoanlEm_PernHotatazclairoPdnroAtEgevecantlicuoaynt,iOoAfngfeincceyoWf.Piedsit1f9ie8c2i.daendPPreoAigqrcuaiamdtse.icAWsOassrehsgmsagmnienoitn,,GuDis.duCeblsicncess,enFI7F1R3A, 3 TOersgt.aniOzEaCtiDonGforuEciofnoordmTiecsetCinogolpoefriCahteimnoincaealnsd. DGeuivdeelloinpeme3n0t5.. P1a9r8s4.. AvianDietary Toc 4 ASAumSbeTsrMciucSeatnaDnideSatoracdrisye,tTyVoolxf.oiri1y1T0eT4se.tstiPsngiwiaatdhnedApvhMaiant,erSPpineaclise.s. A19S87T.M SSttaannddaarrddE4P3r7a.c8t7ic.e AfonrnuCosndBuocctkinogf 5 WNaatsihoinnaglloRne,Dse.aCr.chNaCtoiuonnaclA.cad1e9m96y.PreGsu.ide1f3or5he Care and UseofLaboratory Avimal. 6 HPSatielzpaahdracdnl,pEhCviaEsl.uPaAti19o7n7s,, MAemtahordisnforSCoaclicuTolraTnteLsitCiSnn0gg.nPda.gesMa6t5-e84sI.nAgpuaur.icNTe.ocSoHlEoa6g3n4dy. 7 CStoempmhuanni,caCtEi.on 1978. US. EPA, Environmental Resarch Laborstoy, Dulth,MN. Persons 8 Flaney, DJ. 1971. StatisticalMethods inBiological Assay,2nded. Gif ress, London. 9 Thompson, WR. 1947 Bacteilogical Reviews, Vol 2, No: 115.145. 10. wDiutnhncCto,ntCe:olW..Jo1u9r5.5.AmrA.MSutlaitsp.lesCtoompa5r0i1s0on9s61P3ro1cedure fox Comparing Several Testers 11. Gully, DD. 1990. TOXSTAT Release .2. The University of Wyoming
60708
WILDLIFE INTERNATIONAL, LD
20.
PROJECTNO: 454-102
TABLE| `Cumulative Morality raoMallmard Acute Dictary Toxicity Study with PFOS
"pGrmouep nt
NoDEexpdosPureeePeriBod p
NePoastl-EtxpaosuNree PT eriode
C --_--_m-- ai) "Doy0 Davi Dw? D3W bud Dns Due DT u E Dwi
Contral
0
030 030 030 030 030 03 030 od 030
Treatment
91
010 0M0 wo 010 oo 010 010
183
010 00 010 010 010 00 00
366
010 00 wo 010 @0 wo 010
72
O10 00 00 010 00 00 010
146
010 00 010 010 010 00 00
2
010 00 00 010 00 00 010
S86
O00 00 010 010 wo wo
nn
V0 010 010 010 210 610 310
"The LCST valu was cluebx d28 pp 7 wilh 95% cones mea ST
FS ales scurtia sy ofth smn cl from ay 0 Day 22
oo io 010 ono wo ono mio ono 00 ono m0 20 0 wo sw sno pp STS
%0'709
-_--
WILDLIFE INTERNATIONAL, LTD
a
PROJECTNO 454.102
-- mGaowy Cone
Treatment SI 5 66 2 ue $6
TPAaBgLe1E2 Mean Body Wei(g)fhotm a Malld Ace Disa Toxicity Stuy with PROS
T Bo Expbousese Peed DGS T PopsuEsxposB poeroitod ouiowes
5Mem mns owmew w 5 w
215
5Mem uws omae ee w won
nie
5Mem uWs oonmm
m ne m
m2
5Mem wwoomwasn om wn s hmw
as]
5Me w woww wp wwoww
2000
kMye w boe om ee m aw k
12
5Men wvoawa 3ae wwoeas
"Pe
M3en w 5 e0 ee ns oxd
3a%me
Se A unretMbeyan
14oA7r
12
are
188%
SE FIT
an
15i
O mS0.ealaheo ilmrratyanfw oomeh nd si scoo leo vie ng Dp <le0.0s8(t Dce oser tcs),
C9710
_----------m--
WILDLIFE INTERNATIONAL, LTD "2.
PROJECT NO: 454-102
TABLE2 Page2 Mean Body Weight (g) from 2 Mallard Acute Dietary Toxicity Study with POS.
T Groupeme (ppmai) Dis
PostCEhxapnogseu're Period
Change"
Duis Duysls Diy22 Diyis22
Control Mean EY
Treatment
4010 64506 2o0w
o2) 128%3
ou MseDan 183 MseDan
4550 125 2E0) 3824 a2s 2021
m3 119s8 au1l 1a86
366 sMeDan 52 sMeDan 146 MseDan
373 a0" "3 3624 95179 2216 3820 so6 22
828 2E0l8 L13o5 2033 [o3 ra9
93 Mseoan
awoe satge 2365
La" TC.3)s
586 MseDan
ae s2ee a37
o9ie 2139
un sMeDan
185 nyA 198-
net=,Ncooduadtnaostbeaevlalduuaaetetdobsmtoatrlitsatilceiatllyy ingDummett' es. ""StSattiastiicacly didfiffefreernetnffoommthetcheocnornotollggrroouuppattpp<<0.00.80]((DDuusasr vcsa)s.)
od aseey re
ChanTgoeu(l322)
a2
a3rH ar2 asmtn aiEsS 31506s 4940 "a9 as?-
[li rek
WILDLIFE INTERNATIONAL, LTD
n.
PROJECT NO: 454.102
TAPaBgLeE1S
Mean Feed ConsumptTiooxnic(hygSiudss)wiohmFaOSislrd Ace Diary
-_
Errgimns
I [----a Potpourri
Tew
o y5ew u
uis
remen
a
ws
a
wn 2
306
"
na
n
as
a
0s
PS
"
5 a
0 wn
% u
5s x
_T--e --
naz
WILDLIFE INTERNATIONAL, LTD
"24
ROJECT NO 454
PROJICENO; dsh0
TPAuBgeL2E3 Mean Feed Consumpt"ioTn(obiSxtrudidyycw)ifitrhoPtmFyOMS alAcluteaDirardy -_Experimenl
Gohmieds) Tew
TE Post-ExposE urePeroiodwmS E
o Men
ni
wnn
Tresment
a
"
1
1
16
EY
06
16s
ww
n2
i
mn
16
1
6
0
n
556
i
ww
wn
06
15
_O-- E --
cn0713
WILDLIFE INTERNATIONAL, 110 2s.
PROTECTNNOO: d4s54t.102
TABLE 4 roma MallaGrrdosAscuPtaethDoilcogaircyalTOobxseircvatSitodnsy with PFOS
Binds that died during th coursofte sadly
TTT
_--
Finding
NNTaro
FaSwiea NNsM
Nnee
ECnoapctemepdry GL. Trach, praily cmp
0\
21
? 4
|:1
GGiezazard,cocnmtpetnytsbile sained Intestin contents, lackandtre:
o0
o2
4 1
0o|
KKiedenleypsr,ompilneent
o0 00 2|
SLpleoeonfsmruesyscle mass Spleen, soul and pale
02| o21 2s:
TBv hSpielneen, pale v 0| 2| ai3
cne714
-_--
ol
+
.
.
.
.
1
=
1
1
=
1581381 ccecon ve ve 20 20 vo ve vo vel
1
a8
12810
1
1
1
IZ 8A 222292 29 20 22 22 22 ge 29 09
1
"
1Z are
rE
1
81 5
u
= 0 IM nonnnn
I
1
1
1
2i
v
El? | | 333333
1
Ww EET ml
18 ZEUS
1
SFE 3)
gam 0
Ea I
"Ue
Es ||
munnnnn 335355
AR AX FA RR BR RR ow sal | 43 5a an aa SEAR AG AaliE |
~~. mm a.
158 _BE
33 33 33 a3 33 33 33 =3:1%s |
Ss 2c co on 8% F F AF =5 35 EEE AE EE 55 za18s
188 |
Hi Ise of
SF 22 AN UY IX 9g HR EEE] 55 35 33 33 35 35 535 ss1zzEm
n9715
WILDLIFE INTERNATIONAL, LTD
a.
ArpENDIX
PROJECT NO 454-102
Certificate Of Analysis
FC-95, Lot 217
DTTSTMI arRc9h, 20p 00 mmen ere
a agoer+-- r -- me1w---- ---- -- a 1-- e -- |S-- -- 2---- 1 -- --aks|ox os-- n -- tyors tet og nd ey 0 bil
Le ----
EERE=
60716
_--
WILDLIFE INTERNATIONAL, LTD ES
PROJECT NO: 454-102
APPENDIX II
WILDLIFE INTERDNIAETTIFOONRAMLULLTDA.TIGOANME BIRD RATION"
INGREDIENTS FSionyeBCeoamnMMeael48a% Plrot,ein `PWrhoteeaitnMBiasdes A`Aglfwaalfya MSepeacli,al2,06%0P%roPtreoitnein DGrrioeudndWhLeimyestone EaMsetthmiaonniCnaelPPrheomsix + Liquid `GVLitaFmeirnma(nFdeMrimantecroa)lPremix (sce below) SToolutllodized
PERCENT (6) u306s5 665000 430000 205500 006350 003225 T0001000
VITAMIN AND MINERAL PREMIX
AMOUNT ADDED PERTON
VViitsaammiinn DA; NRiPiaabncotifolntahveinnic Acid VFoiltiacmiAnciBdy BPyiroitdionxine TVihtiaammiinne& MaVnitgaamniensKe(Menadione Dimethylpyrimidinol Bisulfite) ZCionpeper
ordoinn Selenium
270000000000001LCU.U. 406ggrraammss 10 grams 00mgmsg.s 641m2ggrsams
20000112U.grams 10528 ggrraammss 4763grgamrsa.ms 5)15grgarmasm.s. 182 mgs
"ThSehgucarruadnetefiebder.analysis is minimumof27% proein, minimum of 2.5% crude fut and a maximum of Fermentation By-Products (SourceofUnidentified Growh Factors)
Coe?
WILDLIFE INTERNATIONAL, LT E'S
PRROOJJECTNO: dsae1002
APPENDIX I ANALYTICAL METHODS AND RESULTS
00718
WILDLIFE INTERNATIONAL, LTD "30.
PROJECT NO: 454-102
INSTRUMENT.
ANALYTICALCOLUMN: OVEN TEMPERATURE: STOPTIME: FLOW RATE: MOBILE PHASE: INJECTION VOLUME: PFOS RETENTION TIME: IRNETTEERNNTAILONSTTIAMNE:DARD PFOS MONITORED MASS: IMNOTNEIRTNOARLESDTMAANSSD:ARD
Appendix Ill Table |
`Typical LC/MS Operational Parameters aFePewrekrinP-aElcmkearrdAPMIod1e0l0L1C10M0aHsisSgphePcetrrfoomremtaenrceequLiiqpupieddCwhitrhoamPaertkoing-wrEilatmheprh STuIrMb)o.lonSpray on source. Operated in selective ionmonitoringmode. Keystone Betasl Cy column (100 mm x 2 mun .D.,3 um particle size) 0c 100 minutes 0.220 mUminute 72.0% Methanol: 28.0% NANOpure Water containing 0.1% Formic Acid 2s0uL Approximately 7.0 minutes
Approximately 4.8 minutes 498.6amu 4267 amu.
n0719
WILDLIFE INTERNATIONAL, LTD -31-
PROJECT NO: 454-102
Appendix Il "Table 2
Matrix Blanks Analyzed Concurrently During Sample Analysis.
Me Nombw er wc SeeoComennoPFOSn ' or
(#54:102)
Type
(ppm)
MAB-1
`Matix Blank
<1oq
MAB2
`Matix Blank
<10Q
"TqhauenaalyYznoemdito(f0i.00a229tmig ao./(Ln)LOaQn)dwthasd1il1u5tiponpfmbacaat1o.osftehedmautproinx bhleapnrkocsdaumcpatleosflh(Se0i0)lobwestratisanodarnd
6n9'720
WILDLIFE INTERNATIONAL, LTD 2
PROJECT NO: 454-102
Appendix 11 Table 3
Matix Fortifications Analyzed Concurrently During Sample Analysis
Sample NumberTTT
sa-102)
Fortes
TowMAn SA
iass
(ppm GmemmmoHtiesesmred iams
Repceorcveenrted e10r5
MMAaSASS2
1155
16e
89631
MMAASSS
11553300
17151766
893671.
--_--
TT
TTT Standard Deviaetimon =
6e9r9
oNvee
753%
"oie: nRedsumltasnusanl ccaolrcueleaiioonnss foVralnueews h1v1esbuebestnarnocuenpduerdtfoyreweproerigenngerpautrepdoswesi.ng MacQuan vervon 13 sofware
C9721
|
I
I
1
21
12 <1
|
1281 =
21
iz 1
Gi
I
1
ali|
t1g5eeaa=mr osxs
| 1Sxf1 sx
|I
12881 ISia
I
12
I
"1
I
"el
{
TE
i=
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WILDLIFE INTERNATIONAL, 10
.
a
Appendix
PROJECTNO. 454-102
|
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3d
||
050
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----
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0727
--------------------------------------------------------
WILDLIFE INTERNATIONAL, LT
Appendix
ProOvCeTNO; 434102
To o a 7 5 5 "
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-_---
WILDLIFE INTERNATIONAL, (0 "o-
Append
FROCTNO: dst
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WILDLIFE INTERNATIONAL, LTD
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----------------------------------
WILDLIFE INTERNATIONAL, to a
Appendix
PROICT NO 458-102
0
167 ntonahy: 7248cps
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------------------------------
"a WILDLIFE INTERNATIONAL, LTD
PROJECT NO 454102
APPENDIX IV DIET PREPARATION Weiangdvhotmofcontsedn0 prepare et dic:
ConWceontrrations | SuTborse | RTaoiorn
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450g0of0bas0al tion was weighed naaoedHobartmiing bowl,
+The test substance was weighed na ered weigh bost
ApFperndaeirse100 fas tion was taken omthemixing bowl and laced na Waring
Thei test sbnessawoanssacdgdep1e0tto lehnedeermanid the weigh boswesrinsed with aon tion,
Th"eThBeenbdeenrdce owans iwtseeerdbnwlietlndasedddfoorsnspiri,tweit6t0escroinnedsssaondberinagnPslfae1creethhddee mmiixxiinnggbboowwll
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_--
WILDLIFE INTERNATIONAL, LTD
PROJECT NO: 454-102
Las
APPENDIX VI
In`dAicvuitdeuaDlieBtoardyyTWoexiicgihttysS(t5u)dry ovimtah MPaFlOlSa.rd Pag|e
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_--
WILDLIFE INTERNATIONAL, uD
PROJECT NO. 454-102
-a6-
APPENDIX VI
In`dAicvuitdeuaDlieBtoardyyTWoexiigcihttysS8t)udfyrwoimtah MPaFlOlaSrd Page
The"Gmrowe, pms 0
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WILDLIFE INTERNATIONAL, LTD
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APPENDIX VI In`dAicvuitdeuDailetBaordyyTWoexiicgihttysS(t5u)dfyrwoimtah MPaFlOlSa.rd
Page
PROJECTNO: 454-102
TGreowm. pms
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WILDLIFE INTERNATIONAL, LTD
a8
PROJECTNO: 456-102
APPENDIX VI In`dAicvuitdeuaDlieBtoardyyTWoexiicgihtysS(t)udfyrwoimtah MPaFlOlaSrd
Pages
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650737
_--_--mm--m
WILDLIFE INTERNATIONAL, LTD 49.
PROJECT NO: 454-102
APPENDIX VI In`dAicvuitdeuaDlieBtoardyyTWoexiicgihttys(Sgt)ufdryowmitah MPaFlOlSa.rd
Pages
TGroo up pe
Change
Change Toul
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Change
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WILDLIFE INTERNATIONAL, LTD
50.
PROJECT NO: 456-102
APPENDIX VI In`dAicvuitdeuaDlieBtoardyyTWoexiigcihttysS(t8u)dfyrwoiatmh MPaFlOlaSrd
Page
T Grouphm
(ppm
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-_--
WILDLIFE INTERNATIONAL, to sie
PROTECTNOL 454102
APPENDIX VI In`dAicvuitdeuDailcBtaordyyTWoesiicgthysS(t3u)dfywoitmh MPaFlOlSrd
Page?
TE"GGerao)w we Duo bys BCrwungSe pus bCuusgte dTeoul
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_--
WILDLIFE INTERNATIONAL, LTD
52. APPENDIX VI
In`dAicvuitdeuaDlieBtoardyyTWoexiigcihttys S(tgudfyrwoimtah MPaFlOlaSrd Pages
PROJECT NO: 454102
TpeGrrmoeurptal Goma
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--
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_--mmmmmm
WILDLIFE INTERNATIONAL, LD 53.
APPENDIX VI Individual Body Weights (g) froam Mallard `Acute Dietary Toxicity Study with PFOS.
Page 9
(ppGmroiup S86
Bid Dw0 Days ChDaunyg0eS Dus
1 2 105
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PROJECT NO. 454-102
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--_--nmm--
WILDLIFE INTERNATIONAL, LTD
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APPENDIX VI In`dAicvuitdeuaDlieBtoardyyTWoexiigcihttysS(tgu)dr y wito ahMaPlFlm OaSrd
Page 10
PROJECT NO: 454-102
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WILDLIFE INTERNATIONAL, LTD
PROJECT NO. 454-102
"55+
APPENDIX VI
Individual Body Weights (g) froam Mallard `Acute Dietary ToPxaicgiety11Study with PFOS -- Experi-- mental -------------------- (eGprmoaup Bid Dws Doyls ChDawnSgIeS._ D2 CDhaeniges Chang"Teo(u8l22)
0
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WILDLIFE INTERNATIONAL, 10 "56
APPENDIVXI
I"nAdcivuitdeuDailcBtorydyWToxiecityiS9tugdfyiowhmithMPtaFlOlsSa.rd Page12
PROJECT No 45k162
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WILDLIFE INTERNATIONAL, LD
57
PROJECT NO: 454-102
APPENDIX VI In`dAicvuitdeuaDlieBtoardyyTWoexiigcihttysS(t5u)dryowmitah MPaFlOlaSrd
Page 13
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PROJECT NO 454-102
APPENDIX VI In`dAicvuitdeuaDlieBtoardyyTWoexiigcihttysS(t5u)dryowmitah MPaFlOlSa.rd
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WILDLIFE INTERNATIONAL, Lo -59-
APPENDIX VI In`dAicvuitdeuaDlieBtoardyyTWoexiicgihttysS(t9u)dfyrwoimtah MPaFlOlaSrd
Page 14
PROJECTNO: 454102
TheGroup Gpma, sts
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WILDLIFE INTERNATIONAL, LD
PROJECT NO: 454-102
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APPENDIX VII
Feed ConsumpDtiieotanr(ygTboixridciidtayy)StbuydyPewnitfhroPmFOaS.Mallard Acute: Page |
--_E--xperiments] Group
--_--_-- (ppm ai)
Pen
Exposure Period
Post-Exposure Period
Dwos Days
Control
1
109
14s
2
2
10
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9%
132
s
2
100
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-- SMDan Tm 1l0e i[1] m
CnO7A9
_--
WILDLIFE INTERNATIONAL, 10
PROJECT NO 454.102
"a
APPENDIX VI
Feed ConsumDpiicoenygToixicaitFy)aSbecyyPwnithroPmFOaMSallard Acute
--_E-- re Goma ren
EpDoeyoood
PosBeonyeseed
T _-- a MMee2|n m 7n 711008
T _-- 13 MMee2!m a Sa0t 1i1532s
T --_-- 66 MMee2' n w 15058s 111s22
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T --_-- us Mre ee2: m Tohwl y bii0
-- T = MMee2C n a w 5 T g E os ----
sts --
MMee2: m Te 36
oo5
_ T un MMee2: n p 22> 52>
6090750
_--mmm
WILDLIFE INTERNATIONAL, LD
PROJECT NO: 454-102
-62-
APPENDIX VII
Feed ConsumpDtiieotanr(ygThobxiridciidtayy)StbuydyPewnitfhrPoFmOaS.Mallard Acute Page3
-- Experimental Gr_ oup. -- Exposure Per-- iod PostE-- xposare Period
--_--m(ppmai)
Pen Dyes byisam
Control
1
185
159
2
161
182
3
166
199
-- SMDen5 1T3T -- I 2
Gno751
--_----
WILDLIFE INTERNATIONAL, LTD
PROJECT NO: 454-102
-63-
APPENDIX VII
eed ConsuDmipteitoanry(Tgo/ixridc/idtayy)StbuydyPewnitfhroPmFOaSMallard Acute
Paged
--_--Experimental Group.
Exposure Period PostExposare Period
_-- (ppmai)
Pen TDTwyEIs byb@
_--m 91
'
m
198
_-- 183
1
136
204
_-- 366
1
165
9
---- 72 ---- 1 ---- 148 ------ nm --
---- 146 ---- ' ---- 159 ------ 164 --
_-- 203
1
109
132
st6
'
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143
-_-- un
'
106
154
619752
_--mmmme
WILDLIFE INTERNATIONAL, LTD
PR APPENDIX VII
PROECTNO 454102
CHANGES TO PROTOCOL.
change"sT:his study was conducted in accordance with the approved Protocol with the following
1. "The protocolwas amended toad an dina estconcentrationof 10 pma. 2. Tpfherestpmoracoyet.owcaoslcwaas named1nidneddicatto itndhiccaltelcthhsotnbiolfewivlel breocmollBeicrtdedhafrrdoimedalGustnugdyhbeircdos.urseThoeF 3. T4coh0necep2nrniodrtaot1cio2ol8n0swapesprmaem3ce3hn,idneg1d0edt0o,fco9hr,alng1l8t3hh,ee3e0tse6ts,tcos7nuc3bes3nitnr1ac4te6i,opnu2s9r.ogm5i5v60e,na1n0d, (21h01e,74e10w,po0cm,enat1hs6e0n,t3Te2e0os,f
nays. 4. Individually numbered wing bands were use to uniquely deity cach duckling. The protocol
`required leg bands to be used to identify the birds. a
5. BfelsototdubseasmpTlhese pwreoteoccoollldeiccteadteodn tDhaeyhepaanrdiniDzaeyd v2a2cuinsinnoenr-sbwepoauidnibzsedvs5e,ml borosilicate glass 6. Trehceorfdienadl temperature rom one brooder unit on Day and ll brooder unison Day 22 were net 7. TMhaey'a,te1m9o9o)n observations were inadvertently not recorded fr bidsin singlebrooderunt on
OaObbtssheerrevvaatttiiesootnnss ofoTrremccpooemriralltuirredr.ssofrroommthatswneo bbbrrrooooddoerdrlveevevelslwsewrewereemrianaadvsveesrtneonsttlynrnoecteordeecdcooorndoeonidn.BDoaydyy336 oes
00753
_--_--
WILDLIFE INTERNATIONAL, LT "6s
APPENDIX 1X PERSONNEL INVOLVED IN THE STUDY
"ROCTNO: se'in
"The following key Wilde Intermatonal Li.personnelwer involvedintheconductor management ofthis study. (1) Mark Jaber, Wilde Toxcologst @) Joann B. Besvers, Director, Avian Toxicalogy @) Sean. Gallagher, Senior Biologist ) Courtney Casey, M.S. Seni Biologist (5) WillarBd. Nixon, Ph.D, Manager, Analytical Chemistry (6) Tim Kendall Supervisor, Analytical Christy 7) Raymond L. Van Hoven, PhD, Scientist () Ellen Mank, Chemist
Co0754
_--mm--m_--_----
To WILDLIFE INTERNATIONAL, LTD APPENDIXX
REVSTUIDYSSUMEMADRY
sROCT NO. 454.102
charTahcisterrawidazsawratesviiosreendpoonrd971w0i0n,gl3oweitngsusbtsutdaynccoempplertiyon,ofan9dt0h4e%p.urtyThweastdesettsrumbsitnanetcdoe
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pariors ctor of 0.9603 Noate wasmade fo 7ecalult nalyica anda onsenions
or measured concnrions rom rina ve da. As such, minor cor due 0 roundingmaybe
pres. amt
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isthe
revi
summary
pagefromthe
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andthe
most
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AvENED 0755
--_--
WILDLIFE INTERNATIONAL, LTD
PROJECT NO: 454102
"er
REVISED STUDY SUMMARY BASED ON A PURITY OF 865%
Srowson
Comoran
SPONSOR'S REPRESENTATIVE: Ms. SusanA. Beach
DALTocAkATNoDN AoFCOSTPUYDYO,F TaHwE FINALREPORT:
Easton, M-- anand-- 21601
V170.BPLRORT NTOMBEAR TestSUBSTANCE: sow: NcooncwraTATTIeOsTNS: TesToATES
Lexoror esr
ssei02 PFOS (Perflucroctane SulAcifdPootasnsiuimSca) PFOS: ADiaryLCSOStudywiththe Mallard 0,88,176,35.1,703, 141,281,562 and 125 ppm ai.
EBExixpopleeorrgiiimmceeannlstTaalelrTSmeitnaa-amtAiirponrntialMi2aMo2y,any11-49,91914,9919099 nay
TETora SOURCE OFTEST ORGANISMS scm or esr nas:
VTE GsPaton WHrainstoivngeIWLri.n6g1s0,41I-c.3512 10 days tesiin
po
95%cones irs: cNooNcoERNTTARLAITTIOYN: cNoo-nocnmsmeRuvTeIDoEnFFECT.
ma 30md919ppma
141 oma.
351 ppmai
ENDED 050756
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----
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