Document kmb5KnJNKqpadOoQOYvd9n6nD

BACK TO MAIN Study Title Growth and Reproduction Toxicity Teat with N2803-2 and the rreshwater Alga, S1tnaetrua caprlcornutua Data Requirement) Guideline Number U.s. EPA-TSCA, Guideline 797.1050 Authors Timothy J. Ward Peter L. Kowalski Robert L. Boeri Study Completed November* 28, 1995 Sponsor 3m Company Environmental Laboratory 935 Bush Avenue Building 2-3E-09 St. Paul, Minnesota 55144 Tasting Facility : T.R. wilbury Laboratories, Inc. 40 DoakB Lane Marblehead, Massachusetts 01945 T.R. Wilbury study Number 890-TH page 1 of 23 BACK TO MAIN I. GOOD LABORATORI PRACTICE COMPLIANCE STATEMENT Tbia atudy waa conducted according to EPA Good Laboratory Practice Reguiationa (40 era 792), with the following exceptiona. The atability of the teat aubatance waa aaaumed but not verified at T.R. wilbury Laboratories Deviations from the atudy protocol are diacuaaed in section XII of tbia report. Sttuudd;y Director T.R. Wilbury Study Humber 890-TH Page 2 of 23 II. QUALIXT M S U R A N C X TAXEMEMT Submitted byt T.R. Hilbury Laboratories, ine 40 Doaka Lane Marblehead, Massachusetts Certification: T.R. Hilbury Laboratories, Inc. study number 890-TH, Growth and Reproduction Toxicity Test with N2803-2 and the Freshwater Alga, Salenaatrua caprlcornutua, was audited by the Quality Assurance Unit for compliance with the protocol, standard operating procedures, and applicable Good Laboratory Practices (U.s. EPA, 1993). Quality assurance audits were performed and the findings reported to T.R. Hilbury Laboratories management and the study director on the following dates: Aspect Audited Protocol: In-life: Draft Report: Final Report: Audit Date 8 / 3/95 9/22/95 10/25/95 11/28/95 Reported to Study Director 8 / 4/95 9/22/95 10/25/95 11/28/95 Reported to Management 8 / 4/95 11/28/95 11/28/95 11/28/95 Artnur p . Faradica Quality Assurance officer T.R. Hilbury Study Number 890-TH Page 3 of 23 BACK TO MAIN i n . t a b u oir contents SECTION1 I. Good Laboratory Practice compliance statement: zz. Quality Assurance Staterent III. Table of Contents IV. Index of Tables and Figures V. Summary VI. General Information VII. introduction Villa Methods and Materials IX. Results X. References XI. Signature Page XII. Protocol Deviations Appendix A. Water Quality Data from Toxicity Teat PAGE 2 3 4 5 6 7 8 8 13 18 19 20 21 T.R. Milbury Study Nunbar 890-TH Paga 4 of 23 BACK TO MAIN xv. inn or t a a u s aitorxotmu Tabla 1. Characterization of a representative sample of test media and water used to formulate test media for the toxicity test with the freshwater alga, Selenastrur caprieornutum, and H2803-2 Table 2. Test system summary for the toxicity test with N2803-2 and the freshwater alga, Selenastrum caprieornutum Table 3. Cell growth data from the toxicity test with N2803-2 and the freshwater alga, selenastrum caprieornutum Table 4. Average specific growth rate and percent change from the control from the toxicity test with the freshwater alga, Selenastrum caprieornutum, and N2803-2 Table 5. Effective concentrations (EClOs, EC50s, EC90s) from the toxicity test with the freshwater alga, selenastrum caprieornutum, and N2803-2 Table A.l. Temperature measured during the toxicity test with N2803-2 and the freshwater alga, Selenastrum caprieornutum Table A.2. pH values measured during the toxicity test with N2803-2 and the freshwater alga, selenastrum caprieornutum Figure 1. Growth of Selenastrum caprieornutum exposed to N2803-2 for 96 hours 9 n 14 15 17 22 23 15 T.R. Wilbury Study Humber 890-t h iflfi Page 5 of 23 V. s u m m a r y Tha toxicity of N2803-2 to the freshwater alga, Salentetrua CMpricornutua, ia described in this final report. The test was conducted for 3M Company for 96 hours from September 18 to 22, 1995, at T.R. Wilbury laboratories, Inc. in Marblehead, Massachusetts. It was conducted by Peter Kowalski, Jeanne Magazu, Jacqueline Nevius, Keven Stevens, Peter Nevius, Robert Boeri, and Timothy Hard according to the protocol developed for T.R. Wilbury Study Number 890-TH. N2803-2 was supplied by the sponsor. The test was performed under static conditions with five concentrations of test substance and a dilution water control at a temperature of 24 2*C. Nominal concentrations of N2803-2 were 0 (control), 0.99, 2.0, 4.0, 8.0, and 16 mg/L. The dilution water was sterile enriched media adjusted to a pH of 7.5. Cell counts were made daily with a hemocytometer. Exposure of algae to the test substance for 96 hours resulted in a median effective concentration (EC50) of 2.9 mg/L N2803-2, (95 confidence limits > 1.0 to 7.7 mg/L) when the value was calculated using the number of algal cells/ml, and 8.4 mg/L (95% confidence interval 5.9 to 14 mg/L) when the value was calculated using the average specific growth rate. The 96 hour no observed effect concentration (NOEC) is 0.99 mg/L and the lowest observed effect concentration (LOEC) is 2.0 mg/L when calculated using the number of cells/ml and the NOEC is 2.0 mg/L and the LOEC is 4.0 mg/L when calculated using the growth rate. T.R. Wilbury Study Number 890-TH Page 6 of 23 BACK TO MAIN 7P P .B I|II| P|[!1IJJIIMU) IB t V I . OENXRAL INFORMATION | Material Tested! N2803-2 sponsor! 3M Company Environmental Laboratory 935 Bush Avenue Building 2-3E-09 St. Paul., Minnesota 55144 Test Substance , Shipped rromi 3m Company Environmental Laboratory 935 Bush Avenue Building 2-3E-09 St. Paul, Minnesota 55144 Reported Purityi 100% Study initiation Date: August 16, 1995 (protocol signed) Experimental start Datei September 18, 1995 Experimental Completion Date: September 22, 1995 , Study completion Date: November 28, 1995 Archive Location for : the Biological Raw Data and a Copy of the Final Report i T.R. wilbury Laboratories, Inc. Marblehead, Massachusetts H R K B E T.R. Wilbury study Number 890-TH Page 7 of 23 VII INTRODUCTION This study was aponaorad by 3M Company. Tha objective of tha atudy was to datarmina tha 24, 48, 72, and 96 hour effective concantrationa (EClOa, EC50s, EC90a) and no obaerved affect concentration (NOEC) of tha teat aubatance to tha fraahvatar alga, salanaatrua eapricorautum, under atatic conditiona. Tha teat protocol was baaed on U.s. EPA method (e f a , 1993). Tha report containa aectiona that deacribe the methods and materiale employed in the study, and results of the investigation. The report also contains an appendix that presents water quality data collected during the test. VIII. METHODS AND MATERIALS TEST SUBSTANCE : The sample of N2803-2 (T.R. Wilbury Laboratories Sample Number 520) waa delivered to T.R. Wilbury Laboratories on August 14, 1995 in a cardboard box at ambient temperature. It was contained in a 50 ml glass jar. The label attached to the jar included the following information: "N2803-2, See MSDS". N2803-2 (a clear liquid) was supplied by 3M Company, St Paul, Minnesota. Prior to use the test substance was stored in the dark at room temperature. Test substance stock solutions were formulated in dilution water without the use of a solvent. The test substance was assumed to have a purity of 1 0 0 % active ingredient and to be stable under storage and testing conditions. Unused test substance will be returned to the sponsor. DILUTION WATER: water used for acclimation of test organisms and for all toxicity testing was sterile enriched media (U.S. EPA, 1978; T.R. Wilbury standard Operating Procedure number 6 ) adjusted to a target pd of 7.5 with 0.1 N BCl prior to use. Characterization of a representative sample of media is presented in Table 1. TEST OR0ANXSM: .' Algae used for the test (selanaatrum capclcornutrjm, UTEX 1648) was from a culture originally procured from the culture collection of Algae at the University of Texas at Austin and delivered to T.R. Wilbury Laboratories on April 11, 1995. The culture was transferred to sterile enriched media identical to media used for this test and maintained at test conditions for at least 14 days before the definitive test. The subsample of algae used to inoculate media at the start of the definitive test came from a 7 day old culture. Identification of the culture organisms was verified using an appropriate taxonomic key. T.R. Wilbury Study Number 890-TH Page 8 of 23 BACK TO MAIN Table X, chemical characterization of a representative sample of test media and water used to formulate test media for the toxicity test with the freshwater alga, SeXsnastrum capricornuturn. Parameter1 Unit of Measurement Detection Limit Metals Aluminum Arsenic Boron cadmium Chromium Cobalt copper Iron Lead Mercury Nickel silver zinc Nitrate Chloride rluoride Total organic Carbon Total Phosphorus Organochlorine Pesticides Toxaphene Organophosphorus Pesticides Dimetboate TEPP Monocrotophos PCBS mg/L mg/L mg/L mg/L mg/L mg/L mg/L mg/L mg/L mg/L mg/L mg/L mg/L mg/L as N mg/L mg/L mg/L mg/L pg/L pg/L pg/L pg/L pg/L pg/L pg/L 0.1 0.01 0.5 0.0002 0.01 0.03 0.005 0.03 0.005 0.0003 0.03 0.02 0.02 0.05 1 0.1 1 0.03 0.5 2 0.5 2.0 2.0 2.0 0.5 Measured Value ND2 ND ND ND ND ND ND 0.03 ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND Notes: ' 1. Metals, nitrate,; chloride, fluoride, total organic carbon, total phosphorus, organochlorine pesticides, organophosphorus pesticides, and PCBs were measured in deionized water (used to formulate dilution water) that was collected during August, 199S and analyzed by Pace, Inc. as part of routine water quality testing. ' ' . ,' - ' - Y . -V '?'/' . ; .. , 2 . ND not detected at or above the detection limit. T.R. Wilbury study Humber 890-TH Page 9 of 23 TOXICITT TEST: Results of a range finding teat were uaed to aelect the concentrationa for the definitive teat. The range finding teat vaa perforated from Auguet 30 to September 3, 1995, At the concluaion of the teat the number of celle/ml equalled the following percent of the control: 0.10, and 1.0 mg/L - >98, 10 mg/L, 100 mg/L, and 1,000 mg/L <1*. The definitive toxicity teat waa performed from September 18 to 22, 1995, according to T.R. Wilbury Protocol 890-TB (Growth and Reproduction Toxicity Teat with N2803-2 and the Freahwater Alga, selenaatrua caprlcornutum). The protocol, which waa aigned by the study director on August 16, 1995, is based on procedures of the (J.S. Environmental Protection Agency (1993). A summary of the test system is presented in Table 2. The test was conducted at a target temperature of 24 t 2*c with five concentrations of test substance and a dilution water control. Nominal concentrations of the test substance were: 0 mg/L (control), 0.99, 2.0, 4.0, 8.0, and 16 mg/L. A 160 mg/L stock solution was formulated on September 18, 1995 by adding 0.080 g N2803-2 to sterile enriched media rnd adjusting the final media volume to 500 ml. Appropriate aaiounts of this stock solution were added directly to dilution water to formulate test media. Algae were distributed among three replicates of each treatment at the rate of 10,000 cells/ml. The test was performed in 250 ml glass Erlenmeyer flasks that contained 50 ml of test solution. Test vessels were randomly arranged on a rotary shaker adjusted to 1 00 rpm in an incubator during the test (a random numbers table was used to select the location of each vessel). A 24 hour light and 0 hour dark photoperiod was automatically maintained with cool-white fluorescent lights that provided a light intensity of approximately 380 footcandles. The number of algal cells/ml in each test vessel and the occurrence of relative size differences, unusual cell shapes, colors, flocculations, adherence of cells to test containers, or aggregation of cells was determined visually by means of direct microscopic examination witb a hemocytometer. cell counts were made and recorded daily during the 96 hour test. The determination of whether toxic effects were algistatic or algicidal waa performed at the concluaion of the toxicity test (0.5 ml of media from each 16 mg/L vessel was transferred to a vessel containing fresh media without N2803-2 and incubated for 144 hours under test conditions). r : ':r..... T.R. Wilbury Study Number 890-TH Page 10 of 23 BACK TO MAIN Table 2. Teat ayatem summary for the toxicity teat with N2803-2 and the freahwater alga, selenaatrum capricornutum. Species t SeXenaatrum capricornutum Culture Acclimation Period >14 days Teat Duration: 96 hours Teat Veaaela: 250 ml glass flaska Volume of Teat Media: 50 ml Number of Replicate Teat VeaBela: 3 Inoculum: 1 0 ,0 0 0 cells per ml Oacillation Rate: 100 rpm Incubator Temperature Range: 23.4-23.7*C Photoperiod: 24 hours light and 0 hours dark. Light Intenaity: 380 footcandlen Nater Quality Measurements: pH in each test vessel at the beginning and end of the test. Incubator temperature daily. Dilution water analysis is summarized in Table 1. End Points: 24, 48, 72, and 96 hour EC10, EC50, and EC90; no observed effect concentration. Acceptability criteria: , Logarithmic growth at 96 hours and acceptable temperature range. ! 't j -f h\\ I | . ; :: - T-Ra Wilbury Study Ntuaber 890-TH Page 11 of 23 -- ----- ' .' BACK TO MAIN number 124) was determined In each teat vessel at the beginning and and of the teat. Tha temperature in a representative vessel of water incubated with the test vessels was continuously recorded. STATISTICAL METHODSI The average specific growth rata was calculated as tha natural log of the number of cells/ml at 24, ,8 , 72, and 96 hours minus the natural log of the number of cells/ml at 0 hours divided by the exposure period. The percent change from the control was calculated by aubtracting the treatment average specific growth rate from the control average specific growth rate, dividing the difference by the average specific growth rate in tha control, and multiplying that value by 1 0 0 . Results of the toxicity test were interpreted by standard atatiatical techniques. The probit method was used for calculating 24, 48, 72, and 96 hour EC values (and confidence intervals). The probit method was use despite the occasional cautionary note because ECSO values computed by this method were similar to values computed with other methods (Stephan, 1983). The calculations were performed using both the number of cells/ml and the average specific growth rates. Tha no observed effect concentration (NOEC) was determined using a parametric one-way analysis of variance (ANOVA) and the number of celln/ml and the average specific growth rate in each test vessel at the end of the test. BACK TO MAIN IX. RESULT No insoluble material was noted in any teat veeeel during the teet. The algal population in the control teet veaaels grew well, resulting in n average of 3,227,000 cells/ml after 96 hours (Table 3). Hater quality throughout the test was within acceptable limits (Appendix A ) . The range of incubator temperatures was 23.4 to 23.7*C (Table A.l) and the pH of test media was not affected by the teat substance at the beginning of the test (Table A.2). Biological data generated by the acute toxicity test with N2803-2 are presented in Tables 3 and 4. The growth curve for algae exposed to the test substance for 96 hours is presented in Figure 1 (the slope of the dose-response curve is 4.1). The 24, 48, 72, and 96 hour EC10, EC50, and EC90 values for algae exposed to N2803-2 are presented in Table S. Exposure of algae to the test substance for 96 hours resulted in a median effective concentration (EC50) of 2.9 mg/L N2803-2, (95% confidence limits - 1.0 to 7.7 mg/L) when the value was calculated using the number of algal cells/ml, and 8.4 mg/L (95% confidence interval 5.9 to 14 mg/L) when the value was calculated using the average specific growth rate. The 96 hour no observed effect concentration (NOEC) is 0.99 mg/L and the lowest observed effect concentration (LOEC) is 2.0 mg/L when calculated using the number of cells/ml and the NOEC is 2.0 mg/L and the LOEC is 4.0 mg/L when calculated using the growth rate. The determination of whether toxic effects were algistatic or algicidal was performed at the conclusion of the toxicity test. A 0.5 ml aliquot of media from each 16 mg/L vessel was transferred to a vessel containing fresh media without N2803-2 and incubated for 144 hours under test conditions. The number of cells/ml equalled 190,000 cells/mL, indicating that the effect of N2803-2 at this concentration was algistatic rather than algicidal. BACK TO MAIN Tab la 3. call growth data from tha acuta toxicity taut with N2803-2 and tha freahwater alga, fialanaatrum capricornutum. Nominal concantration of N2803-2 (mg/L) rep. Number of Calla/ml x 1 0 J (hour) 0 24 48 72 96 0 (control) 0.99 2.0 4.0 8.0 16 i 2 3 mean % control 1 2 3 mean % control 1 2 3 mean % control 1 2 3 mean % control 1 2 3 mean control 1 3j mean control 10 116 10 88 10 94 10 -- 99 -- 10 84 10 98 10 112 10 98 100 99 10 92 10 96 10 84 10 91 100 92 10 112 10 86 10 96 10 98 100 99 10 80 10 84 10 76 10 80 100 81 10 48 10 44 10 36 10 43 100 43 480 2,040 3,180 388 1,760 3,620 364 1,680 2,880 411 1,827 3,227 ---- -- 286 1,950 3,480 368 1,840 3,600 450 2 , 0 1 0 3,820 368 1,937 3,633 90 106 113 376 1 , 1 2 0 2,940 370 1 , 0 1 0 2,540 366 850 1,840 371 993 2,440 90 54 76 240 496 492 252 432 714 236 512 536 243 480 581 59 26 18 176 262 168 240 254 180 188 246 158 201 254 169 49 14 5 114 82 58 102 80 56 118 92 70 111 85 61 27 5 2 T.R. Milbury Study Number 890-TH Paga 14 of 23 BACK TO MAIN BACK TO MAIN Table 4. Average specific growth rate and percent change from the control from the toxicity test with the freshwater alga, Selenastrum capricornutum, and N2803-2. Nominal concentration Of N2803-2 (mg/ I s ) Average specific Growth Rate 24hr 48hr 72hr 96hr Percent change Prom Control 24hr 48hr 72hr 96hr 0 (control) 0.99 2.0 4.0 8.0 16 0.096 0.077 0.072 0.060 0.095 0.075 0.073 0.061 0.092 0.075 0.064 0.057 0.095 0.066 0.054 0.042 0.087 0.063 0.045 0.029 0.061 0.050 0.030 0.019 1 3 -1 -2 4 3 11 5 1 14 25 30 9 18 38 52 36 35 58 68 BACK TO MAIN Table 5. Effective concentration* (EClOs, EC50s, EC90s) iron the toxicity teat with the freshwater alga Smlntttrua oaprlcornutum, and N2B03-2. Time EC Value (mg/Z>) 95 Percent Confidence Limita (mg/L) Calculated Using Cells/ml 24 hours 48 hours 72 hours 96 hours EC10 as 4.1 EC50 >16 EC90 m >16 EC10 m 1 . 2 EC50 m 7.1 EC90 - >16 EC10 m <0.99 EC50 m 2 . 8 EC90 - 8.4 EC10 EC50 m EC90 m 1.4 2.9 6.0 0 - 9.0 7.7 - >16 >16 <0.99 - 1.6 6.0 - 8.6 >16 <0.99 - 1.9 1.1 - 5.8 4.5 - >16 <0.99 - 2.4 1.0 - 7.7 3.5 - >16 Calculated Using Growth Rate 24 hours EC50 - >16 48 hours EC50 m >16 72 hours 96 hours EC10 m 2 . 2 EC50 m 11 EC90 m >16 EC10 m 2.3 EC50 m 8.4 EC90 m >16 -- -- 1.6 - 2.8 9.4 - 15 >16 <0.99 - 3.6 5.9 - 14 >16 T.R. Wilbury Study Number 890-TH Page 17 of 23 BACK TO MAIN X. RXrCIUMCM Stephan. C.E. 1983. Computer Program lor calculation of LC50 values. Personal Communication. 0.8. EPA. 1993. 40 CFR Part 797. Toxic Substances control Act Test Guidelines; Final Rules, section 797.1050. U.s. EPA. 1993. 40 CFR part 792. Toxic Substances Control Act (TSCA); Good Laboratory Practice standards; Final Rule. T.R. Wilbury Study Number 890-TH Page 18 of 23 ! [ X I . XOMXUItS P M I BACK TO MAIN BACK TO MAIN xiz. protocox.Mammana amt deviations PROTOCOL AMENDMENTS! The protocol was amended to spacify tha nominal concentrationa for tba definitiva teat. PROTOCOL OBVIATIONS! Concentration #1 ia 0.99 mg/L rather than the 1 mg/L specified in Protocol Amendment #1. This deviation did not affect the outcome of the teat and no other deviations were made from the protocol. T.R. wilbury study Number 890-t h Page 20 of 23 BACK TO MAIN Appendi! Al WATER QUALITT DATA FROH TOIICITT TEST T.R. Wilbury Study Number 890-TH Page 21 o 23 BACK TO MAIN . Table A.l. Temperature measured during tha toxicity tnat with N2803-2 and tha freshwater alga, Helenastrum ctprlcornutum. Hour of Exposure Temperatura of Incubator (*C) 0 23.6 24 23.7 48 23.4 72 23.6 96 23.7 T.R. Wilbury Study Number 890-TH Page 22 of 23 BACK TO MAIN 2.0 4.0 8.0 16 2 3 1 2 3 1 2 3 1 2 3 1 2 3 7.5 7.5 7.5 7.5 7.5 7.5 7.5 7.5 7.5 7.5 7.5 7.5 7.5 7.5 10.7 10.5 9.3 9.1 9.0 8.7 8.7 8.7 8.6 8.5 8.5 8.4 8.4 8.4 T.R. Wilbury Study Number 890-TH Pago 23 of 23 /U I /CUUI cttv m u r n o i i m l --LMu u n n i u n ARCHIVED FINAL REPORT LAB REQUEST NO. N2803 REQUESTOR NAME: SAB/GOEBEL/PICKETT DEPARTMENT: 3048 PROJECT NO: BI703004UQ DATE RECEIVED: 08/01/1995 DESC: FC-143 AND RELATED PRODUCTS ECOTOX WORK CONTRACT LAB(S): TRWIL EXP COMP DATE: 10/04/1995 DATE COMPLETED: 03/27/1996 PROJECT LEAD: PHONE NO: 3M FAX NO: 651-778-6176 BACK TO MAIN Samples received from S.J. Martin. Samples 1-5 sent to TRWIL on 8/11/95. An additional 4L of sample 5 sent on 8/22/95. Sample 1 = Tetrabutyl ammonium hydroxide, 40% in water. Sample 2 = L-13492 (45% solids, 27% water, 27% IPA) Sample 3 = FC-26, 100% solids. Dissolve in 50:50 water:IPA solution. Sample 4 = FC-143, 100% solids. Sample 5 = L-13856, 15% solids. Sample 6 = L-13856, diluted by a factor of 15, tested as an effluent. Sample #1 (Tetrabutylammonium hydroxide) - a new bottle was ordered from Aldrich because TR Wilbury needed more sample. This was lot 05922BN, red label, cat. # 17,878,0. sab 9/11/95 NOTE: All data for sample 3 (FC-26) is reported in terms of FC-26, and not as a 50/50 mixture with isopropanol. sab 11/21/95 NOTE: For sample #5, only a range-finding test was run for toxicity to algae. The requestor canceled the request for testing so no definitive test was run. The results for the range finder are as follows: Test Cone. Percent Difference in Avg. Specific Growth Rate mg/1 0.1 1 .0 10 96-hrs Expsure 2 18 4 100 1000 32 100 EC50 (95% C.I.) 160 (100 - 1000) mg/L. sab 2/16/96 For N2803-6, the testing was canceled before a definitive study with fish could be completed. Results reflect testing with a single concentration of 13% v/v, which showed no mortality after 96-hours, sab 3/14/96 ----------------- ----------- - - - - - - - - - - - - ---------- - ----- ----------- ----- ------- - y - - - - -------------- SAMPLE DATE CODE DESCRIPTION RESULT MDL or (95% C.I.) 1 08/11/95 TB NH40H Lot 10317KQ TRWIL 48-HR-EC50 *DAPHNIA ACUTE TEST 27 MG/L (23 - 32) V-.VJIYIn u I L A D U M I M 04/01/2001 LAB REQUEST NO. N2803 SAMPLE DATE CODE ENVIRONMENTAL LABORATORY ARCHIVED FINAL REPORT CONTINUED DESCRIPTION RESULT BACK TO MAIN 2 MDL or (95% C.I.) 1 08/11/95 TB NH40H Lot 10317KQ TRWIL 96-HR-LC50 *FISH ACUTE TEST - 96 HOUR 720 MG/L TRWIL IC50-ALGAE *ALGAE ASSAY - IC50 3.7 MG/L (600 - 1000) (1.8 - 7.7) 2 L -13492 Lot 2 TRWIL 48-HR-EC50 *DAPHNIA ACUTE TEST TRWIL 96-HR-LC50 *FISH ACUTE TEST - 96 HOUR TRWIL IC50-ALGAE *ALGAE ASSAY - IC50 62 MG/L 960 MG/L 8.4 MG/L (36-100) (830->1000) (5.9 - 14) 3 FC-26 Lot 135 TRWIL 48-HR-EC50 *DAPHNIA ACUTE TEST TRWIL 96-HR-LC50 *FISH ACUTE TEST - 96 HOUR TRWIL IC50-ALGAE *ALGAE ASSAY - IC50 360 MG/L 140 MG/L 90 MG/L (300 - 500) (110 - 180) (63 - 130) 4 FC-143 Lot 427 TRWIL 48-HR-EC50 *DAPHNIA ACUTE TEST TRWIL 96-HR-LC50 *FISH ACUTE TEST - 96 HOUR TRWIL IC50-ALGAE *ALGAE ASSAY - IC50 720 MG/L 740 MG/L >1000 MG/L 5 08/16/95 L-13856 As-is (600 - 1000) (660 - 830) (-) TRWIL 48-HR-EC50 *DAPHNIA ACUTE TEST TRWIL 96-HR-LC50 *FISH ACUTE TEST - 96 HOUR TRWIL IC50-ALGAE *ALGAE ASSAY - IC50 190 MG/L (130-600) >1000 MG/L (-) SEE COMMENT ABOVE 6 L-13856 1:15 diluted TRWIL 48-HR-EC50 *DAPHNIA ACUTE TEST TRWIL 96-HR-LC50 *FISH ACUTE TEST - 96 HOUR TRWIL IC50-ALGAE *ALGAE ASSAY - IC50 * = CONTRACT LAB DATA 1.2 % V/V (1.0 - 1.7) >13 % V/V (-) TESTING CANCELED