Document kmN14odovXbp1Z1pBGbLzX07E

ACTIVATED SLUDGE RESPIRATION INHIBITION TEST TEST SUBSTANCE ldent4: Perfluorooctanoiaccid,ammonium salt;may alsobe referredto as PFOA ammonium saltA,mmonium perfluorooctanoateF,C116, FC-126, FC-169, or FC-143. (Octanoicacid, pentadecafluoro-a,mmonium salt,CAS # 3825-26-1) Remarks: 3M productionlotnumber 390. The testsample isFC-126, a white powdery solid.It'psuritywas notsufficientclhyaracterizedt,hough currentinformationindicatesitisa mixtureof78 - 93% testsubstance and 7 -22% C5, C6, and C7 perfluoraonalogue compounds. METHOD: Method: OECD 209 Test type: Staticacute GLP: No Year completed: 1987 Analyticalmonitoring:Dissolvedoxygen concentrations. Test organism source: Activatedsludgemixed liquorcollectefdrom the metro Wastewater Treatment Plant,St Paul,MN. Used the same day as collection. Test conditions: Dilutionwater: Aerated,distilledde,ionizedwater Synthetic sewage: per OECD 209 Test vessels: Not stated Number of concentrations: 5 plusone reference substance and 2 blank controls. Test temperature: 20 - 21 OC Mixed liquorSuspended Solids: 2.7g/L pH of Activated Sludge Mixed Liquor: 7.8 (initialn)d 8.0 (final) pH of1000 mg/L testconcentration7:.4(initiaanld)8.1 (final) Exposureperiod:30-minuteasnd 3-hours Referenceand testsolutiopnreparationA:lltestsubstancesolutions were createdby adding individuawleightstosolutionscontaining284 mL Di water,16 mL syntheticsewage feed,and 200 mL inoculum.The pH of these solutionswas then adjustedto7.0using 1N NAOH. Reference solutionswere createdusinga 500 mg/L stocksolutionof3,5- dichlorophenol Elementbasis:Respiratiionnhibitaisondeterminebdy dissolveodxygen consumption. RESULTS Nominal concentrations:Two Blank controls1,00, 180,320, 560, and 1000 mg/L testmaterialsolution. Percent Inhibitionin RespirationRate Test Substance Concentrations (mg/L) Control 1 Control 11 100 180 320 560 1000 30-Minute Exposure 0 0 1.1 0 7.0 11 29 3-Hour Exposure 0 0 4.2 4.7 14 17 F- 38 Element values: 30-minute EC5o > 1000 mg/L 3-hourEC50 = > 1000 mg/L Controlresponse:Adequate- 2.2% differencienrespiratiroanteofthe two controlsforthe 30-minuteexposure and 0.8% differenceinrespiration rate of the two controlsforthe 3-hour exposure. CONCLUSIONS The testmaterialinduced 38% inhibitioinnrespirationrate at 1000 mg/L after3-hours of exposure. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability:Klimisch ranking 2. This study meets the criterifaorquality testing.However, itlackscharacterizationof the testsubstance purityand analyticalconfirmationof testsubstance concentrations. No resultswere given forthe reference compound. REFERENCES This studywas conducted by the 3M Company, Environmental Laboratory,St.Paul,MN, Lab Request number E1282. OTHER Last changed: 5/24/00 ENVIRONMENTAL LABORATORY ACTIVATED SLUDGE MIXED LIQUOR Type Test Ae--4ivA4,F,l Date 7 Analyst *-,iol SourceA,lpi-rn Date Obtained 'P)ail+- -Ci-aul.p MAI Date Used C;Lo - Mixed Liquor Suspended Solids (MLSS) 7 g/L Adjustment Alon-f &/L PH.:rnii-I&I.:7,9 Comments Fi nAl = ig,12 Test Substance Sample Description F 0- - 1 112L + @-3q o Sample pH4 hig@@ l@no,iz,etv_!W)Lz 1-,1,Adj ustment All -t -tn Comments II)eld..Ikt<, Reference Substance Sample Descriptioq_ Sample pH Comments -Ni/ %nlocc,12kP vin Adjustment-- n C., -)n Exposure Conditions Test Duration Test Temperature Comments --fe&+ :3- 4tuY-c, 9@o - ;t I oc r&vi (A-, i L P, Form 166204-0 PWO Sample Description P'6 1-'DBfiC) pou.@&,y Environmental Lab sloat-e- Work Sheet Lob Request No. Page I Of -A-) Date: Ll - @,,o Analyst: ico 7' qo /@o .3aeo nv.L DT- LL.@&+er- AAA,,,L ini I .991 rNL .4y; n Pt@[ i f Ito ML *iicre-6-1-al -Ttl&Co lu0A &Latd I- oloo -Z ARI 110 + &400 ^I Soo Vel. 4- ;tg V + 16 + 0c) 57C)C) + 4- IR4 + + 0(3 + oo 560 5-")Z) 4- 4- App r-P'U :Ec e:.'1 ';6 1ut COt4p IP4.0 1)4 f e-c+-t- L A 1 4 e-, -t 4, pd 7,-0 U 11-4.6 1 )j Ali @c,@,VrPrt ii:ii-6 Lp_ Fjlgi-l Fr- qVO4 Ln 4. 1n I -Ie a LL)tt4@et-t)ceci &Y-)7-S2-7 ti ACTIVATED ENVIRONMENTAL SLUDGE RESPIRATION LABORATORY INHIBITION RAW DATA LR )-C TEST OECD #209 Teat Substance: F C L , 4. Source of Activated Sludge Mixed Date Race ad.: Analyst: -"-e. 'ie -gCj 9-7 30 - MIN. EXPOSURE Test Substance Concentration (mg/L) 0.5 DISSOLVED 1.0 1 2.0 3.0 (A-@tA<4@ -T[g A-men+ Pl&vii- &t- A4,V Date IJeed:- g-,--,LO-R7 OXYGEN CONCENTRATIONS ag/L TI14E-MINU'fES 4.0 1 5.0 6.0 1 7.0 8.0 1 9.0 10. 60 CONTROL I I0c) 6.k 13.3 2.611 .1 q-8 q.( 3 .11@.7 @-o 1.3,o.15' 51 3.5 @.g ;z.1 1.3 Q.-7 3,@ !3. 5 0-ci CONTROL Il 3 - HOUR EXPOSURE DISSOLVED OXYGEN CONCENTRATIONS mg/L_ Test Substance Concentration (mg/L) 0.5 1.0 2.0 1 3.0 4.0 1 5.0 6.0 7.0 1 8.0 9.0 1 10.0 CONTROL 1 3. 3.o 9 61le (o,31,---),s q,;?q..?@ '-3.-3 9.0 7 ti 7 5-1.7, (0-1 5 15.1 0 .71.-kA 3. b zzid 9,,@ 7cl 75-7,1,6,7 &.9\S-8 5.-3 [q.1 AO 7.,@,(.0.1b.3 S@ 5,q lq.1 y,s-@10 oil 7.7 7,4 17, @.-7 6--7 At) CONTROL 11 1-7,917.A pH of Activated Sludge Mixed Liquor pH of highest concentration tested Test -Temperature .9,z-) 'g,1 11c -Tn%*io.1 5. 1 7,1 o Fin4l c> FinLt ACTIVATED ENVIRONMENTAL LABORATORY LR- 9 ck Date_ 'j-,;t,0-9'7 Analyst SLUDGE RESPIRATION INHIBITION TEST OECD #209 CALCULATIOti OF RESULTS p Test Substance 4o 30 - MINUTE EXPOSURE Test Substance Concentrations (mg/L) CONTROL 1 r.r_ @zn - Oxygen Uptake Rate R 0, 7 .112, o - 7 o F@., 0 - 7 1q CorrecivL Z Inhibitionin Respiration Rate ti'7 -7 7.10 0 q Q.lt 9 0. 14914 .-AONTROL 11 70 k 1 Percent Difference in Controls (2) 0. I. 7 k-@ EC50 (95 Z Confidence Limits) mg/L 3 HOUR EXPOSURE Et6b= Aoio (1350 51,9c,) Test Substance Concentrations (mg/L) CONTROL I c,06 Oxygen Uptake R Rate ft iCacc4atA % Inhibition in 4-0 0 a c Respiration Rate 0 1.3 1-, ..) -4?51-4p o 0.1 ika 9 149C)-oo.@ q33*-@ t,--,14 Li 0 qt75- -0..314"5 '7 17 3 @Z CONTROL II O..EAQ -7 Percent Difference in Controls(2) o - @90 EC5'0(95% Confidence Limits) mg/L 3AC" Sk@, 1000 m,@IL (1) Z Inhb,tou /RC, + RC 2 E,C Rs (il,5o-H7AO) RC I + RC 2 p@5 -I- - pra (2) Z Difference RC, - RC@ x loo Cl + RC2@ -7 - Where: R - Oxygen,.,UptakReate (mg 02 i.L/Kin.), s - Test Substance cim Control I c Control 11 f)= IT.c-otq@s7 - 2.0&c The two B'KK Control respiration rates should be within 15% of each ?Ibii,