Document kaj3qDqMBLQ7vXRKgZbkZaq6V

ACUTE TOXICITY TO AQUATIC INVERTEBRATES (E.G.,DAPHNIA) TEST SUBSTANCE Identity:Perfluorodecanesulfonataem,monium salt;may alsobe referred toas PFDS, FC-121-X,or3M Sample E2112 and E2566-1.(1Decanesulfonicacid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,9,9,01-0h,e1n0e,i1cosafluoro-, ammonium salt,CAS # 67906-42-7) Remarks: Test sample isa mixtureofthetestsubstanceinwaterand 2propanol,1-(1,1-dimethylethoxTyh)i.s mixturecontainsapproximately2226% testsubstance,33-37% propanol,1-(1,1-dimethylethox0y.)5,-1.5% perfluorononanesulfonataem,monium saltand 38-42% water).Allvalues reportedrelatetothismixture.No calculationwsere made toadjustfor the actualconcentratioonfthetestsubstanceinthetestsample. Test sample was from 3M productionlot1. METHOD Method: EPA 660/3-75-009and APHA (1980)Standard Methods forthe ExaminationofWater and Wastewater Test type: Staticacute GLP: Yes Year Completed: 1988 Species:Daphnia magna Analyticalmonitoring:pH, dissolvedoxygen,temperature.Test substanceconcentratiownas not monitored. Statisticamlethods: EC50values calculatdeusingBinomialmethod Test daphnid source: ABC Laborato(iesIn-houseculturesC,olumbia, mo Test daphnid age atstudy initiation<:24-hours Test conditions Dilutionwater:Well water Dilutionwater chemistry (from a representativesample): hardness: 246 mg/L alkalinity 178 mg/L pH: 8.0 Stock and testsolutions preparation:Not given. A cloudy appearance and surfacefoam presentat preparationinthe 560 and 1000 mg/L concentrationwsas not noted atthe 24- and 48-hour observationperiods. Exposure vessels:250 mL glassbeakers containing200 mL Of testsolution. Number ofreplicate2s: Number ofdaphnidsperreplicat1e0: Number ofconcentratiofnisv:pelusa negativceontrol Waterchemistrdyuringthestudy: Dissolveodxygenrange(0-48hours): 8.7- 9.2mg/L (controelxposure) 8.6- 9.2mg/L (1000mg/L exposure) pH range (0-48hours): 8.2- 8.2 (controelxposure) 8.2- 8.4 (1000mg/L exposure) Test temperaturerange (0- 48 hours) 19 - 20 OC Element basis:mortalitaynd sublethaelffects RESULTS Nominal concentrations: Bk control1,00,180,320, 560, 1000 mg/L Element value: 24-hour LC50 = 200 (100-320)mg/L 48-hourLCso= 130 (100-180)mg/L 48-hourNOEC (sublethaelffects=)< 100 mg/L Sublethaleffectsincludedquiescenceand/or daphnidstendingtothe bottom oftestvessels. StatisticaElvaluation:The LC5()valueand 95% confidenceintervawlas calculatedusingthe binomialmethod. Allelement valuesbased on nominalconcentrations. Mortalityof controls:None Remarks: Values reportedareforthetestsample (mixture).No calculationwsere made toadjustfortheconcentratioonfthetest substance inthetestsample. Cumulative percent mortality: Nominal Test Conc., mg/L 24-hours 48-hours Neg. Contmi 100 180 320 560 1000 0 0 0 0 35 100 100 100 100 1 100 100 100 CONCLUSIONS The testsample 48-hourEC50 forDaphnia magna was determinedto be 130 mg/L witha 95% confidenceintervaolf100 - 180 mg/L. Testingwas conductedon the"as-soldm"ixtureofthesubjectmaterialin water(-22-26% subjectmatherial)The valuesreportedapplytothat mixtureand notdirectltyo thesubjectmaterial. Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St.Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking2. Thisstudy,whilewellconducted,lacks analyticadlata for:determinatioonfthetestsubstanceconcentratioinnthe testsolutionsa;nd determinatioonfthe sample purity.Because a mixture was tested,care must be takenwhen interpretitnhgeresults.The solvent inthe mixturecouldhave enhanced or restrictebdiologicaulptakeand/or toxicitoyfthe fluorochemicaclomponent. REFERENCES This studywas conducted by ABC LaboratoriesC,olumbia,MO on behalf of the3M Company. OTHER Last changed: 5/18/00 SPONSOR 3M CoWanY Attn: Mr. 14ohamedEinabaraiy EE & PC Enviro=ental Labs Building 21-2W-05, 900 Bush Avenue St. Paul, 14innesota 55106 STLIDY TITLE Acute Toxicity Of E2566-1 tc E!p.hnia magna DATA REQLTIR7--%T OECD Guideline ?02 AUTHOR Scott Frazier Biologist STLDY COMPLETED 0% January 9, 1988 PER FORMING LABORATORY Analytica 1 Bio-C he n"try:@ j[,@@@,or'atoriesI,nc.. A qua tic Toxic.@iogy Division 7200 Eai3t ABC Lane P.0 BiD:i1097 Col umb a M"sotiri,,.65205 ABC LABC iRAToRY PROJECT ID Paige.I..of@45 Submitted By: Analytical Bio-Chemistry Laboratories, Inc. 7200 East ABC Lane P. 0. Box 1097 Columbia, Missouri 65'.05 (314) 474-8579 Prepared By: Si@6tt Frazier Biologist II/Study DirectL;r Approved By: Date Alan D. Forbis Supervisor, Invertebrate/ Bioconcentratio,nInvestigations Date. Kent Ediger. Quality Assurance offic6r @,Date, :dab28 Carl M. Th OMP soir Date Aquatic Toxicology Manager 'ABC'LABS TABLE OF COHTENTS Page No. LIST )F TABLES . . . . . . . . . . . . . . . . . . . . . . . . . 36614-4 PROJECT PERSONNEL . . . . . . . . . . . . . . . . . . . . . . . 366'A.4-5 INVERTEBRATE ACL@TE TOXICITY CO%iPE.NDIL.4. . . . . . . . . . . . . 36614-6 SUMMARY . . . . . . . . . . . . . . . . . . o . . . . . . . . . 36614-7 IN"rRODUCTIO,%. . . . . . . . . . . . . . . . . . 36614-8 METHODS A.ND @Lk-th.RIAIS . . . . . . . . . . . . . . . . . . . 36614-S I. Test Organisms . . . . . . . ... . . . . . . . . . . 36614-8 II. 17-1. Test Prccedure . . . . . . . . . . . . . . . . . . . . 3661.'4-8 Test Cam-,iound . . . . . . . . . . ... . . . . . . . . 36614-9 TV. St,-,,Iist4-.cs. . . . . . . . . . . . . . . . . . . . . RESULTS . . . . . . . . . . . . . . . . . ... . . . . . . . . . .6.614-9 LITERATURE CITED . . . . . . . . . . . . . . . . . . . . . . . . 3661&4-13 Q'@ALITY ASSURA.NCE STATMIR-%T . . . . ... . . . . . . . . . . . . 36614-14 STUDY COYXLI,@-NCE STATL '4EN"r. . . . . . . .. . . . . . . . . 36614-15 APPENDIX RAW DATA . . . . . . . ... . . . . . . . . . . . . . 36614-16 ABC. 1-,kBS 036614-3 o f -45 LIST OF TABLES. Table 'No. PaRe No. I Chemical Characteristics of Well Water" Used bv ABC's Acuatic Toxicolog%, Division. . . . . 36614-10 Acute Toxicitv of E2566-1 to Daphnia inagna. 36614-11 3 Percent Mortality and Water Quality_ Measurements During the Acute Toxicity Test of E2566-1 to Dapl-,nia=agna . . . . . . . 36614-1-1 -,ABC'LABS.,@#j6@146@-4 PROJECT PERS014NEL The Study Director of the pro'ject for Analytical Bio-Chemist ry Laboratories, Inc. was Scott Frazier, Biologist I!. Supervising the conduct of tt-e study for 3M,Company was Mr. Meha?n d Eluabarawy. The following ABC personnel assisted with various phases of the study. Name Jeffrey Latham Michael Ruddy Scott Frazier Title Biological Technician I Biological Technician Il Biologist II. Initials '4 .. ABC- IV INVERTEBRATE ACUTE,TOXICITY COMPMOILK Subject: "Acute Toxicity of E2566-i'@t'oDaphnia magra. ABC Study Number 36614.,. f Sponsor. 3M Company Testing Facility: Analytical Bio@-Chemistry Laboratories, inc. Aquatic Toxicology Division P.O. Box 1097,,:i. Columbia. Missouri.@ 65205 (314-474-8579) Location of Original Raw Data and FInAl.Report: 3Y. Company E7r.& PC Environmental Labs Building 21-2W-05'*..900Bush Avenue St. Paul, Minnesota.. Test Material: E.1566-1 Test Concentrations: Control, 100. 180 320, 560 and 1000 mg/l. D4@lutionWater: 246 mg/l (hardn 8.0 (PH). -i78 mg/l (alkalinity), Test Dates: Initiation Jan"'r'y@'-,7 1988 Temination-- Januai-Y,@,@91i,l98 ....... 'k,-:@l@'ti!ngtohf@ Study: 48 hours 24-hour LC 50 24-hour NOEL @oo 100 in 48-h our LC 50, *"48-hour NOEL 13u.,mg L 00-320 mg C.I.-: 100-189,.mg/1), Test Species: Daphn a magna Source' of 0 rganismi;: 'ABC-iib'o"r'4'-@'c?"'nr-,h-oIuese:-'i cultur6.": ,@.,Age-,of Organisms at Study Init,i -%. First-instar <24 hou is'old) T@"S 36614@@6 -of 45, . SUMMARY The acute toxicity of E2566-1 to Daphmia magna was assessed using the methods outlined by the Committee o'nMetho@s for Toxicity Tests with Aquatic Organisms. Water quality parameters of temperature, dissolved oxygen and pH were measured at the termination o.' the test and were within acceptable limits. The results of the 48-hour static Daphnia magna toxicity study are summarized' below. All reported.values were based upon nominal::concentrations. Compound E2566-1 24-Hour LC 50 (95Z C.I.) 200 mg/l (100-320 mg/1) 48-Hour LC50 (95"bC.I.) 130 mg/l, (IOC-180 mg/1) The no-effect level observed. for E2566-1 at 24 hours was 100 tmg/l based on the absence of all abnormal effects. The 48-hour no-effect level was estirated to be <100 mg/l since abnormal effects were observed in all treated test concentrations. e oil= I 'ABC LABS -'136614:.@7.,,'o4f5.,, INTRODUCTION This definitive static bioassay was performed by the @Aquatic Toxicology pivision of Analytical Bio-Chemistry Laboratories. Inc.. Columbia, Missouri, for 3H Company, from January 7, 1988 to January 9. 1988. The purpose.of this test was to determine the 24- and 48-hour levels for E2566-1 to Daphnia magna. The study was performed using LC AB@ 0Protocol #7806 as approved by Mr. Mohamed Eluabara-.y, 3M Company, on January 7, 1988.'. FEI-HODS AND MATERIALS. The procedures for static bioassay, as described iu.@Ke@@ds of with Fish, Macro invertebrates and Amphibians (1) Acute Toxicitv 'Tests a=ination of Water and Wastewater (2), were, and Standard Methods used in this experi-Tnt. I. Test 6rganisms The Daphnia magna used in the test were cultured'at the ABC facilities in well water (Table 1). The adult @daphnids were..fed algae (Selenastr= caprico.-nutu=)at least@ every ...,.thredeays prior to tescing aT:dsupple=ented with a suspension .of Tetramin*, cereal leaves, vita--insolution and yeast. The daphni,ds were identified to species using the taxonomic key 'presented by Pennak (3). Il Te s-t Procedure static ]Daphnia bioassay was conducted in7 250.-@zl,,g@lass beakeris@containing 200 =I of daphnid culture/test 'vater."-i.I.Test vessels were covered with loose-fitting petrl dish'*@,.,covteor,s, protect -igainstccntaminat,i6n".dtiring minim:ize evaporation and a-':', the These vessels were kept at '@O temperature,c.ontrolled area. The lighting viis,,,'maintaintaitd.'-. on a 16-hour daylight.I -': @' h "3u-mlnuce simulated dawn and dusk periods.. M,,@i'niii@l'-.-iai%ge-findienxgperiment w @,.'.teDraipb n ia --eac@ in exposure concentratio S' C 'd s@: mg,tI.-,,.,,.F-r*otmhese'results, five trea eii in duplicate'vitliten -Daphnia (firstinstarless t old) @ei biilker.were selected for the definiti46'@,'biosaasy. ese-.-,,concentrjitionser@, a -logarithmic series ranging from. 10 '/I7-and- in6lWid -a control. An ;iial . we re observe-.a o'uce",:every h'ours for morti b licli@@ , offects-'us'cli--as surfacing, clumping of the -daptiiiide-,.',,,together., and, daphnid s.:t'ending to the bottom of ..test: 'Water ry,@parameters of 'temperature, were,-.@measured:., the..control i ow, m""iddii"@And., ''at@;.'O':and 4 8 h ours.'' ABC LABS@#36 -614 III. Test Co=pound The E2566-1 sample was received on December 29, 1987 as a light brown liquid and was stored at room temperature in darkness. Sample purity was unspecified. Test concentrations were prepared based on total product. All standard weights and dilutions can be found in the Appendix Daphnid cultureltest water was used in the preparation of all working stock solutions. IV. Statistics RESLILTS Statisticalanalysis of the concentration vs. efiect data (generally mortality) was obtained by e=ploying a co=puterized LC 10 program developed by Stephan et al. (4). This program ca culated the LC statistic and Its 95 percent confidence limits using the b,-nomial, the moving average and the probit tests. However, if no mortality occurred or if a dose response cculd not be demonstrated over,,a reasonable range (-37 to @,63%) an LC50 and/or its 95 percent confidence limits could not be calculated. Three different methods of anal-yzing the data were used since no one method of analysis is appropriate for all possible sets of data that may be obtained. The method of calculation selected for presentation in this report *ias that which gave the narrowest confa'.dencelimits for the LC 50 (4, 5) although all three models are valid. Table 2 preaents the calculated LC values and 95-percen-. confi- dence intervals for E2566-1. The 24- 52 an 48-hour LC values were 200 and .130 mg/l, respectively. 52 All res-ilts'were base on the nominal concentrations of 100, 180, 320, 560 and '1000 mg/l. The no-effect concentration based on the absence of mortality.ind abnormal effects was 100_,mg/lat 24 hours. At 48 hours the no-effect level was estimated to. be -<100 mg/i since all treated concentrations exhibited abnormal, effects. The abnormal effects observed."dur:ing .,Studyw.ere mortality,.,, -:quiescence and/or dapl-.nidstending to.'the,:,biittoofm, test, vessels. A"'; .:-cloudya:ppearance. and surface foam pres"*nt at preparation. in the 560 and @i@:--,:100i0n-g./l concentrations, was not notea "l'at the 24- an d 48-hour ..,oDservation periods. 'Table 3 presents the percent m6'r ity- d,water qual'ityparameters,.,, '.,.measurde..,@duringthe test. The dissolved ,@okygen concentrationison range between -8.6 and 9.2 mg/l., These valLu" ri@iesented.. 99 and,103-@erEen -,,.saturaotni at 20 and 19 *C. respect Lve,l@'-,';:,"(correctedfor...16ci 'altituditialpressure), ant4.we re conside red,-,adiiquatefor testin9' (1) ....Tti@'@pH@@-@aluesof the test solutions --"ra6g'i@dfrom- 2 to 8 4 an d wer c on sistent with the control'' The'@t dy was con ductea e Gco d Laboratory, Pra6ti@ e Regulations (6) and the f inal.,,teo6rt.@wa-srevieved, bry, BJ6-chemistry- Laboratories' 'Quality@'Assis@incei',;nit data were provided to 3M Company, B O-Chemisiry Laboratories. copy.*r.e,taiuidd'at Anal@t ical r ABC, -LABS,,'.#36614-9-of-"45;---.:'- TABLE I Chemical Characteristics of Well Water Used by ABC's Aquatic Toxicology Division. Parameter Temperature. ..Dissolved Oxygen a (caco 3 Alkal,init@.(CaCO') 3 ...Conductivity .@@Total Organic Carbon SUSP ended Solids num, Arsenic,,"'; Boron Cadmium Chromium, @o ba 60]?per ead Mercury N'ickial -.Si Concentration .17 22-@b 7.4 - 9.1 ppm b 7.6 - 8.4b 206 - 275 ppm b -.336 ppab 500 @650 urhos/@@ .1.4 ppac (0. 9,-77.2. 8 @ppm) d b -ppm -ppb ppb 2 25 PPM pp@ 7 @pp b; @ppb.@@.-;p3pb' 14 -@ppb 0* 43 ppb' -Meg sure d';,organoph@sp@ orus Paz sure organochlorine i@:Ld'ipsi." PCB's ;41'0, .......... . ..... @Represimuts-.@-iseasonavari a'tion -dif a will not exceed 0 C verage o 11-1@7ellwate r @v aluis from a,.moutul,to , ua 7 ef 9 7,'@@7 Fr d -ota1.,mout y rangeof organJLc,c'arbOril sami)le'.dates;:@',Trace:elements':u=ne 3@ 1987,;-.'@oriaiiophbi@bit organoc orin'e'analyses May' -28 1987 10 TABLE 2 Acute Toxicity of E2566-1a to Daphnia magna CompoundE2566-1 24 hours 2.00 (106-320)' LC 50 (=g/1) 48 hours b 00-180) a Bioassay conducted at 20*C (t2.0). b 95% confidence limits..@@ I The 24-hour no-effect concentration was 100 mg/l. based an the absence'@ of all abnormal effects. The 48-hour no-effect ccncentration was all estimated to be <100 mg/l since' ibnormal effects were observeil?',iii, test concentrations. All values were 'rounded to two significant figures foll64in9 the technique provided in ABC S.O.P. #8.7. LC 50 calculated using: Binomial Il'it'-hod ... 'A' 'iBd -@-LA NMI TABLE 3 Percent Mortality and Water Quality Measirements During the Acute Toxicity Test of E2566-1 te Daphnia magna. Nominal Concentration (MR/1) Control 100 180 320 560 1000 Percent - Mortality 24 hr 48 hr 0 0 0 0 .35 100 100 100 100 100@ 100, 100 Water Qualit- 0 hours Temp. a OC D.O. b mg/l 19 9.2 19 9.2 48 hours Temp. D-.O. c PLH OC MR/i PH 8 :'@20 ,,,8.7 8.2 8.2 20 @8.7 8.2 19 9.2 8.2 20 8.8 8.4 19 9.2 8.2 @..20, 8. 6 8.4 a Temperature mercur y :thermometer. I bDissolved.oxygen concentrations YSI 54 ARC dissolved oxygen meter. cpH Co rnin 9 146 pH/mV,'meter wi tb;Beckman 39831 electrode IIOTE.' Diss6lvid -oiygen.satu ratiidus at, the'_Itest:t,empeiZat1ii.1eit.6,f."1,9-'aiLd.,@.,,,, tee fok@@-'altiitnua 20*C are,8 .9 ind'.8.7m,g/l.-resiDectively'(correc i)ressure @at ABC.:@ibs) .. ........ Y, -ABC ..L"S 36 LITERATURE CITED (1) Committee on Methods for Toxicity Tests vith Aquat ic -Organism.,,. Methods of Acute Toxicity Tests with Fish, Macroinvertebrates and Amphibians. 1975. U.S. EPA, Ecol. Res. Ser. 660/3-75-009.- (2) American Public Health Association. 1980. Standard Methods for the Examination of Water and Wastewater. 15th ed. Washington, DC. 1134 p. (3),Pe=ak, R. W. 1978. Freshwater Invertebrates of the United States. ' John Wiley Sons, New York, 2nd,ed. 803 p. Stephan, C. E., K. A. Busch, R@ Smith, J. Eiirke@and R.@ li@.-..Mdrews. 1978. A Computer Program for Calculating an.LC50.@._ .:;-Environmental - Protection, _Agency, Duluth, Mi=isota, vri iicat.i'on, manuscript, August, 1978., (5) Stephan, C. '1977. Yeihods' for Calculating an LC -50*. 65-84 In F. L. Hayer and J. L. Hamelink (eds.). Aquatic Toxicology'@and Hazard Evaluation. ASTI.4 Special Technical Publication' 634'-' @,AST4.@ @@Philadelphia. (6) Organization for Economic Cooperaiioli and Development. Guidelines for Testing of Chemicals,.'.Principles,of. Good Practice Annex 2, C(81) 30(Final):7-28. OECD :Ir.,@aboratory 17, QUALITY ASSURANCE STATEMENT Quality Assurance Statement for report r'36614 entitled, "A,@ute Toxicity of E2566-1 to Daphnia magna." for Mr. Mohamed Elnabar avy 3M "any, St. Paul, Minnesota-. In accordance with ABC Laboratoriest intent that all 'studies -conducted at our facilities are designed and function in conformance with good laberatory 'practice regulations and the protocols for'.,@,individual laboratory studies, an inspection of the final report for.E2566 l'@was conducted and found to be in an acceptable form by a member of our Quality Assurance Unit. A procedure audit was conducted on Deceni@er.14 1987. Results were reported, to the study director on thi @."'sameday 'and to management on December 36i:'1987. A* final inspection of.,'all@,dataand records on January 13, 1988. Indicated that the report submitted -to you is an accurate reflection of the study as it was conducted,:by.,ABC Laboratories. Should you have any questions relating zc the info.-mation.pr6vided in this statement or the function of our Quality Assurance Unit, please contact the Quality Assurance,Unit at ,,ourccn,.renieLce. Kent Ediger Quality Assurance Officer .74 STUDY COMPLIANCE STATEKENT Study Compliance' Statement for ABC report #36614 Toxicity of E2566-1 to Daphnia magna," for Mr. Mo Company, St. Paul entit'lad,@-_@A cute d pAn,*16A 3M In accordance vith' ABC Ubo-ratories' intent that - ;a'!,,',a,quatic toxicity tests conducted.-by our facility follow,good laboratory prac- tices, ABC's.study,4irector,for the above test herein conflras*,that the study was conducted:@ .. @comp iance with the OECD Guidelines..fo'r..,Testing of Chemicals, Principliis@'.of Good Laboratory Practice A=ex 30.(Final):7-28. 2,, C(SI) All origi=l "'ra'w';'dawtear.e provided -to 3M compa77' retained at Analnical Bio-Chemistry Laboratories d a copy Scott Frazier ABC Study Director;. - Date ABC'-LAB' ACTIVATED SLUDGE RESPIRATION INHIBITION TEST TEST SUBSTANCE Identity:Perfluorodecanesulfonatea,mmonium salt;may alsobe referred to as PFDS, FC-121-X, or 3M Sample E2112 and E2566-1. (1Decanesulfonicacid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,9,9,100-,h1e0n,e1icosafluoro-, ammonium salt,CAS # 67906-42-7) Remarks: Test sample isa mixtureofthe testsubstance inwater and 2propanol,1-(1,1-dimethylethoxyT)h.is mixturecontainsapproximately2226% testsubstance,33-37% propanol,1-(1,1-dimethylethoxy0).,5-1.5% perfluorononanesulfonatea,mmonium saltand 38-42% water).Allvalues reportedrelateto thismixture.No calculationwsere made to adjustfor the actualconcentrationofthe testsubstance inthe testsample. Test materialwas from 3M productionlot1. Note: Only the summary page was availablefor thisstudy METHOD Method: OECD 209 Test type: Staticacute GLP: No Year Completed: 1987 Analyticalmonitoring:Dissolvedoxygen concentrations. Exposure Period: 30-minutesand 3-hours Measurements: Dissolvedoxygen depletionrateafter30-minutesand 3hours ofexposure. RESULTS Nominal concentrations: Blank control,100, 180, 320, 560, 1000 mg/L, induplicate. Element value: 30-min EC50 > 1000 mg/L 3-hour EC50 = 780 (630-1050) mg/L Allelement values based on nominal concentrations. Statisticamlethod: Probitanalysis Control response: The largestpercentdifferencebetween controluptake rateswas 7.5%; thus the two controlswere within15% ofeach other. ReferencesubstanceresultsN:otavailable Remarks:Testinwgas conductedon the"as-solmdi"xturoefthesubject material(-22-26% subjectsubstance inwater).The valuereported appliestothatmixtureand not the testmaterial.No attemptwas made to determinethe impact ofthe presence ofthe organicsolventsorwhat portionofthe toxicitcyan be contributedtothe perfluorodecanesulfonate. CONCLUSIONS The 3-hour EC50 forFC-121 was determined to be 780 (630 - 1050) mg/L Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St.Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking4. Most ofthe supportingmaterialforthis studyismissing. Because a mixturewas tested,care must be takenwhen interpretintghe results.The solventinthe mixturecouldhave enhanced or restrictebdiologicaulptake and/ortoxicitoyfthe fluorochemical component. The studylackedanalyticaclonfirmatioonf concentrations and characterizatioonfthe sample purity. REFERENCES This study was conducted by the 3M Company, Environmental Laboratory,St Paul,MN, Lab Request numberE2112, 10/30/87 OTHER Last changed: 5/18/00 LWII#J*UNI'AL IAL'a?AT(]Ry.'AL'TIVATED SLUDGE RESPIRATION INUBITION IW #209 CALA]IATION OF RESULTS Tcst Substunce FC-I;tl-y liit L E EXPOSURE Test Substance Concentration(m IL) MNmoi. Ovgen Uptake Rate URRP@ oI 77 Aq 7 2 7"@ 7 1;21 L Rt Cc@rrected(l) to 2eC Y7 Z t, LI,2q 9 L 40. 37.@2 1 (4:z LR Da 30-.97 I I:nhiibb-itionin -sPirlrat2tjtionRaie(2) Percent Difference in Controls(3) Confidence Limits) mg/L P(poq rpp Test Substance 'Concentration (mg/L) COMR OxYgen Uptake Rate R tl I c -7 R Cor,rected(l) to 2e C q i;@-l7 t Inhibitoin in Res@)irationRate 7b r r0; (3) 7 im ts-)@g/L- rrection s (Mi92 /L-min.) ,qribere. yxygl@ 0.4 @-2 x @iori@, 2 ,rate mg 0 /L- @it.e7mi CO i. C u 5, ,-20 Zi" ce wo' cc)4tm sh bidd,bi,' 4:"z 14 rares--,' the MICROBICS MICROTOXC TOXICITY TEST TEST SUBSTANCE Identity:Perfluorodecanesulfonatea,mmonium salt;may also be referred to as PFDS, FC-1 21 -X,or 3M Sample E2112 and E2566-1. (1Decanesulfonicacid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,81,09,,190,,10-heneicosafluoro-, ammonium salt,CAS # 67906-42-7) Remarks: Test sample isa mixtureofthe testsubstance inwater and 2propanol,1-(l,1-dimethylethoxy)T.his mixturecontainsapproximately2226% testsubstance,33-37% propanol,1-(1,1-dimethylethoxy0).,5-1.5% perfluorononanesulfonatea,mmonium saltand 38-42% water).Allvalues reportedrelateto thismixture.No calculationwsere made to adjustfor the actualconcentrationofthe testsubstance inthe testsample. Test materialwas from 3M productionlot1. Note: Only the summary page was availableforthisstudy METHOD Method: MicrobicsMicrotoxODProcedure Test type: Staticacute GLP: No Year Completed: 1987 Species: Photobacteriumphosphoreum Analyticalmonitoring:None Statisticamlethods: Microbicscomputer softwarepackage Test organism source: MicrobicsMicrotox@ Reagent Test conditions:Per Microbicsmethod Replicates:Two RESULTS Nominal concentrations:125,250, 500, 1000 mg/L Test Sample Element values: 5-minuteEC50 =640 (605-670))mg/L 15-minuteEC50 =290 (260-310)mg/L Allelement values based on nominalconcentrations. CONCLUSIONS FC-1 21 exhibiteda 15-minute EC50 forPhotobacteriumphosphoreum of 290 mg/L witha 95% confldenceintervaolf260- - 310 mg/L. Testingwas conducted on the "as-sold"mixtureofthe subjectmaterialin water (-22 -26% subjectmaterial)T.he valuesreportedapplytothat mixtureand not directltyothe subjectmaterial. Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St.Paul,Minnesota,55133 DATA QUALITY Reliability::Klimischranking4.Most ofthe supportingmaterialforthis study ismissing. Because a mixturewas tested,care must be taken when interpretintghe results.The solventinthe mixturecouldhave enhanced or restdctedbiologicaulptake and/ortoxicitoyfthe fluorochemical component. REFERENCES This study was conducted by the 3M Company, Environmental Laboratory,St Paul,MN, Lab Request number E2112. OTHER Last changed: 5/18/00 'To)(icttc@y 40, pC- IAI;( (ql-A601-0&f.Zq-sL40i y PecCent L ol-5h Lo- 15-,VIIAI 500 (q.5 @C) F N q Y6 @70) 0 O'Ci, - '@"-3A0iiAf@7E1-x0 ---------ClC) 61 b t I -tton rz in --- ACUTE TOXICITY TO FISH TEST SUBSTANCE Identity:Perfluorodecanesu lfonate,ammonium salt;may also be referred to as PFDS, FC-121-X, or 3M Sample E2112 and E2566-1. (1Decanesulfonicacid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,9,9,100-,h1e0n,e1icosafluoro-, ammonium salt,CAS # 67906-42-7) Remarks: Test sample isa mixtureofthe testsubstance inwater and 2propanol,1-(l,1-dimethylethoxy)T.his mixturecontainsapproximately2226% testsubstance,33-37% propanol,1-(1,1-dimethylethoxy0).,5-1.5% perfulorononanesulfonate,ammonium saltand 38-42% water).Allvalues reportedrelateto thismixture.No calculationwsere made to adjustfor the actualconcentrationofthe testsubstance inthe testsample. Test materialwas from 3M productionlot1. Note: Only the summary pages of thisreport were available. METHOD Method: OECD 203 Type: Staticacute GLP: Not known Year completed: 1988 Species: Pimephales promelas Supplier:ABC LaboratoriesIn-houseculturesC,olumbia, MO Analyticalmonitoring:Dissolvedoxygen,temperatureand pH. Test substance concentrationwas not measured. Exposure period:96-hours Statisticamlethods: Binomialmethod. Length and weight: Average length 18 mm. Average weight 0.087 g Test conditions: Dilutionwater chemistry: hardness: alkalinity: pH: 44 mg/L 30 mg/L 7.5 Stock and testsolution preparation: Detailsnot available.All testvesselscontainingFC- 121 containeda thinlayeroffoam at0- hours. The amount offoam increasedwiththe concentrationN.o foam or filmwas presentafter24-hours. Number of fishper concentration:ten Number of concentrations:fiveplusa negativecontrol Water chemistry during the study: withinacceptablelimits throughoutthe study. Temperature during the study: 22 + 10C RESULTS Nominal concentrations: Bk control3,.2,5.6,10,18,and 32 mg/L Element value: 24-hourLC50= 26 (18-32)mg/L 48-hourLC50 = 24 (18-32)mg/L 96-hourLC50 = 13 (10-18)mg/L 96-hourNOEL = 3.2 mg/L Allelement valuesbased on nominalconcentrations StatisticaElvaluation of Mortality: Binomialmethod used to calculatLeC5o valuesand corresponding confidenceintervals. CONCLUSIONS The testsample 96-hourLC50 forfatheadminnow was determinedto be 13 mg/L witha 95% confidenceintervaolf10 - 18 mg/L. Testingwas conducted on the"as-sold"mixtureofthesubjectmaterialin water(-22-26% subjectmaterial.The valuesreportedapplytothat mixtureand notdirectltyothesubjectmaterial. Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St.Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking4. A portionofthefinalreportismissing. Because a mixturewas tested,care must be taken when interpretitnhge results.The solventinthe mixturecouldhave enhanced orrestricted biologicaulptake and/ortoxicitoyfthe fluorochemicaclomponent. Characterizatioonfthetestsubstancepurityand analyticamleasurement oftestedconcentrationwsas lacking. REFERENCES Thisstudywas conductedby ABC LaboratoriesC,olumbia,MO on behalf ofthe3M Company. OTHER Last changed: 5/18/00 -1 SPONSOR 3M Company Attn: Mr. Mohamed Elnabarawy EE & pc Environmental Labs Building 2l-2W-05- 900 Bush Avenue st Paul, 14innesota 55106 STUDY TI-LE Acute Toxicity 0';--566-1 to Fathead Minnow (pimeohales CLIRF,4ENT OECD Guideline 1203 AuTlioR Jane F. Bo*.,man Biologist II STUDY C O'-MIPgLLEtT7EDl1"1 OuNll January 15, 1988 '@PERFOMING '1..aOIULTORY 'i@'al.Bio-L;nemsiti@y Laboiratories;,@,.: T ox icolog3 -"72,00 East@ABC ne P.O. -Box 1097 'Cblumb :La. Missouri, 65205"@,,' ------ IFWLI@WoltATORY'@PROJLI 7r'ID I wi Submitted By: Analytical Bio-Chemistry 7200 East ABC Lane P. 0. Box 1097 Columbia, Missouri 65205 (314) 474-8579 Laboratories, 'Inc. :Prepared By: JAne H.-Bowman iiolo9ist@II/Study -Director Date Laura Mullen Biologist I Date.@. .:Approved By:@ es P.: Su-igert. Ph-M. t-up#evrlLrisor';,'-FiAschui e dh hi11 If M uckl er illr'X.., surance 5uperv:lsor ;Al"@:"..@,,@ V; :@ThompsonQ 6logyj@anageF.@,@: '6bi i Date"@". ate FISH ACU.TE TOXICITY COMPE.NDIUH Subject: Acute Toxicity of E2566-1 to Fathead minnow promelas),4*-ABC Study Number 36615. -Ei-Mep"les- Sponsor: 3H Company' Testing Facility: Analytical ,o'Ch*miqtry Laborato ries, Aquatic Toxic'ologyDivision P.O. Box Columbia Missouri 65105 (31.1*-474-8759 Location of Original Raw.@Dats.:aud'.Final Report: 3M Compan y St.'Paul, Minnesota 55106 Test Material: E2566-1 Test Concentrations: Control, 3.2,-5.6. 10. !e and 32 11 Dilution Water: 44 mg[l (hardness) 30 mg/l (alkaliiiity) -p@t4,14,@@,*@,@@@',@' Test Dates: Initiation anuary, 11, 1988, Termination"-','Janixaiy15, 1988 Length of Study - 9 6-ho4r. LC 50 0' '96-7h our-,0 32 96-b6ut Slop e,o :the-iDose-Raisp-on so Line Ifat, pec 10s: procaelii), @i organlsmsi-ti,,,,ABC'Labibr ouso' culture-- neaa--weight mean,'I: 0 08 FP @...i ;4@ @S@"'#36615 '6 SUMMARY The acute toxicity of E2566-1 to fathead mitmov (Pime@hales proselas) was assessed using the methods outlined by the Committee on Methodb for Toxicity Tests with Aquatic organisms. Water . quality'@',". ra of ttmperature, dissolved oxvgen and pH were measured: throughout the test and were within acceptable limits. Culture and. zcclimatictirtco.-dsIndicated the fiih were in good condition for testing. Ttie t;tudy was conducted at the following nominal concentrations of F2566-1: 3..". 5.6. 10, 18 and 32 mg/l. A thin la.ver.of.@.foam vas present in all chambers at 0-hour. " The a=unt of foam increa@sed.',i@lth". tht concentration. No foam or film was present after 24 hours. Ten fish. with a mean weight of 0.087-(;tO.027) g and a mean stardard -ltngth''@' rf 1,4(!1."()mm.-were exposed.to each'test concentration and con:tro.i T@ic results of the four day@ static f-sh @@oxicity'study 'using il@li6#)-atre summarized below. The 24-. 48- and 72-hour LC values were also det.-rmined. The 96-hour slope of the dose response 11noe was 32.' Compbund 96-Ecur LC 50 (95t C.I.) E2566-1 13 mg/l (10-18 mg/1) Also. the results. indicated a.96-hour, no-observed,effeci.':'@&ohceri-.@'@ t.rationcould be estimated at@ 3.2 mg/l, which was based on tb6";-la6k of :mortality and abnormal effects Abnormal effects of moriality'.'@ floss..,o *quilibrium, light discoloration -and or fish* on the bottom' "'o' chamber were observed clurini e@cp osure peiqod',.,In"@hii::@65.,'@ arid.3 mg/l teiit TABLE 2 Acute Toxicity Of Ez-c,66-1 to Fathead Minnow (EIRSpbales pr=elas) Compourd a E2566-1 LC @in 50 24 hours- 26 b, (18-3.) . -iiigramstliter (ppm) 48 hours 96 hours 24 b -32) 13 bt N 10 fish per concentration. .,.a Bioassay as conducted at 22*C (t 0.087,(:tO.027)g and 18 (ti.7) mm...' @io"n fish,weight and length,'@ b 95% confidence limits. The 96-hour no-observed effectlci)n'@entraton @ould be estiwted*'@'at@ 3" mg/l,, based on the lack of mor talIO: iiid-a.bno r--aleffects at thik@!,-IOWest ------atra,@ion't.ested. al6pe'of. the 96-hour dose',ri ponse,, curve.,a s @'squares regress on 1y sis was:'. c a culate values were.1roun ded to t folloving iL, ra.Leul-ated using -X" i@:,,,Binom:Lal,@.Metdho r-zi, @riat@--Metlaid S 366 .5 1 7-11'@ f