Document kVgO8zYbjj3xp7NJ1kXZOqan
M A A 6 - OZH3
ACUTE TO XICITY TO AQUATIC INVERTEBRATES (DAPHNIA MAGNA)
TEST SUBSTANCE_______________________________________
Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-602. (1Octanesulfonic acid) (CAS # 2795-39-3).
Rem arks: The 3M production lot number was 501. The test sample is FC602-X. Current information indicates it is a mixture of 1% PFOS, 12% diethylene glycol butyl ether, 61.05% water, 20% ethylene glycol, 2% Sultone foamer, 1% sodium octyl sulfate, 1% sodium decyl sulfate, 1% magnesium sulfate, 0.7% xanthan gum, 0.1% starch, 0.1% n-(3-chloroallyl)hexaminium chloride, and 0.05% tolytriazole.
The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect the toxicity o f the fluorochemical component o f the test sample.
METHOD:________________________________________________________
Method: OECD Method 202, U.S. EPA Method EG -1.
Type: Acute static
GLP: No
Year com pleted: 1988
Species: Daphniamagna
Supplier: In-house cultures. Originally obtained from U.S. EPA ERL, Duluth,
MN.
Analytical m onitoring: Temperature, pH, and dissolved oxygen.
Exposure period: 48-hours
Test organism age: <24-hours old
Statistical method: LC50 values calculated using a computer software
program.
Test conditions:
Dilution w ater: Carbon-filtered well water
Dilution w ater chem istry:
Hardness:
240 mg/L as CaC03
A lk a lin ity :
220 mg/L as CaC03
Conductivity: 500 pmhos/cm
Lighting: Not given Stock and test solution preparation: A primary stock solution of 1000 mg ~ test substance added to 1L well water. Exposure concentrations prepared by addition of stock solution to dilution water in exposure vessels. Initial pH of the stock solution was 8.2. Exposure vessels: 250 mL Pyrex glass beakers with 200 mL exposure solution and a water depth of 6 cm.
002114
Num ber o f replicates: 2 Num ber o f daphnids per replicate: 10 Num ber o f concentrations: five plus a blank control
W ater chem istry during the study: pH range (0-48 hours): 8.1 - 8.2 (control exposure) 8.1 - 8.3 (1000 mg/L exposure) Tem perature range (0-48 hours): 20-22 C Dissolved oxygen range (0-48 hours): 8.7 - 9.2 mg/L (control exposure) 7.5 - 9.2 mg/L (1000 mg/L exposure)
RESULTS
Nom inal concentrations: Bk control, 100,180, 320, 560, and 1000 mg/L.
Elem ent values: 48-hour EC50 = 1000 mg/L
Element values based on nominal concentrations
Rem arks: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone.
CONCLUSIONS______________________ ____________________________
The FC-602 48-hour EC50 was determined to be 1000 mg/L, the highest concentration tested. Mortality observed was 50%.
Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133
DATA QUALITY___________________________________________________
Reliability: Klimisch ranking - 2. This study meets the criteria for quality testing. However, the study lacks information on purity of the test substance and it lacks analytical confirmation of the amount of fluorochemical proportion in solution.
REFERENCES___________________________________________________
This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number F 1 1 5 1 ,1988.
OTHER_____________________________
Last changed: 6/26/00.
002115
ENVIRONMENTAL LABORATORY DAPHNIA TOXICITY DATA SHEET
ut/l5l_________
.-
EC-\b03L~ X *-'9V>+UJoL^rkYL
TEST
&0&C__ CHB1ICAL TESTED (Hi-ZLaa-SoTt - g )
LOT S o l.
TEST ORGANiai.b^fiVmva m a y SOURCE US fcPA g-ft,L.
M / J DATF. REC'D ^-/H ~8k
!C)AGE OF TEST ORGANISMS:<24 hrs. old instars (neonates). NO. ORGANISMS PER TREATMENT
TEST VESSEL: 250 ml PvrexR glass beaker TEST SOLUTION VOLUME: 200 ml WATER DEPTH: 6 on
~/S~ &QTIME STARTED./. n n p ^ _______ DATE STARTED. 3 DATE COMPLETED 3~i7~8&
DILUTION WATER Carbon-filtered.we ll water CTem>..jLp_.C, D.0- 7> mg/Lf pH I )
ANALYST^nI^w ^.^..^ Q /\rL _ , F.C^(9S% Confidence Limits) lb o a 1 ^ ) 1
so'ZnMtr<
Test Initial Total
24 Hours
48 Hours
Vessel Cone. Amount Total No. pH D.O. Total No, pH D.O.
No. mg/L Added Dead
mg/L Dead
mg/L
. X Control
M /O o A O
I- ) 80 31t
1-3 3*1o J-H 'S ' looo
(p H ua
Ak
O
D
O
O
o
O g.a. g.7 O #.a, g . o o 9 .c o 3.A $ . 0 o A 5 7.9 T J 7.(d
Test Temp. C Initials Date
ft 3~U>-gp
A. 3 -1 7 S
Comments:
SQ\uAlffln..J.. Jflgq r ^ / ^ t W l fOAifr ^ ja i-Z , a i if i
/\pp^csnily. C am p tetely fo W i-b lf l u it(u., ii k U flig f_____
vu u a lp ii.
- f f r& -------- -------------------------------------
Test methodology conforms with the following two test protocols: (1) OECD #202 "Daphnia sp.,Acute Immobilization Test and Reproduction Test". (2) US-EPA #EG-1 "Daphnid Acute Toxicity Test".
002116!
ENVIRONMENTAL LABORATORY DAPHNIA TOXICITY DATA SHEET
LRJ UL5LL
TEST TYPEJ 8 - K. Aci >te.
FC- i C- X
iUa.vcr 4TC. PWs
CHB4ICAL TESTED tm-atcoa-aon ^ LOT
\
XTEST ORGANISM D a . p W a m & y f l SOURCE U S rP4 ftU \ W,r M
DATE RECD g -1V - g 4
AGE OF TEST ORGANISMS:<24 hrs. old instars (neonates). NO. ORGANISMS PER TREATMENT l O
TEST VESSEL: 250 ml PyrexR glass beaker TEST SOLUTION VOLUME: 200 ml WATER DEPTH: 6 an
TIME STARTED-J*PC pv^_____ DATE STARTED. 3 ~ I S '
DATE COMPLETED 3 -I
DILUTION WATER Carbon-filteredm i x water (Tempo s c. P.0. 9- a mg/i,, pH g. \ )
ANALYST,^y-soo^. CX
EC5Q(95% Confidence Limits)Jr.-}.Q Q Q __ In n a ma I I iv ^ A u r^
vTo Vnrvo.iTfy
Test Initial Total
24 Hours
48 Hours
Vessel Cone. Amount Total No. PH D.O. Total No, PH D.O.
No. mg/L Added Dead
mg/L Dead
mg/L
JF
a -\
2 -i
H
5-5
Control
lo o 20 l&C ?>!c
?>Ao L> y
SkO l i z OOO &
O O
O
O
O
0
O .1 8-7
O & x 2.3,
C 8. 2[8.| o $ .o O 8 . * 7,g
f T S 3 7,S'
Test Temp. C Initials Date
&d<
Z-lte-B8 SM3> M B .V 17-SO
Comments: * &4ock
icon
/- cell
__ 4<xtu ! > \ l ~ ITM
A p p a re n tly completely m i.scAlg u;Hrk.JLLjit u : A r--------
X h t-ftd j P H~- > 3 ) ...........
... .
I.
-II !--
Test methodology conforms with the following two test protocols: (1) OECD #202 "Daphnia sp., Acute Iimiobilization Test and Reproduction Test". (2) US-EPA #EG-1 "Daphnid Acute Toxicity Test".
O O ^ llV
03/24/1988
ENVIRONMENTAL LABORATORY PINAL REPORT
1
LAB REQUEST NO. P1286
REQUESTOR NAME: RR ROBIDEAU/SAB DEPARTMENT: 0222 PROJECT NO: 06 DATE RECEIVED: 03/02/1988 DESC: CARBON-FILTERED WELL WATER - FEB
1988
CONTRACT LAB: PACE
CONTRACT LAB COST: 144
3M E-L HOURS:
1
EXP COMP DATE: 03/23/1988
DATE COMPLETED: 03/23/1988
ORIGINAL SOURCE OF, WELL WATER, 3M WELL #2, ST. PAUL, MN.
SAMPLE DATE
CODE
DESCRIPTION RESULT
MIN DET LIMIT
1 03/02/88 ROOM 4
WELL WATER
COD
SPECIFIC CONDUCTANCE
AMMONIA NITROGEN - as N
__*PH _
(
SOLUBLE FLUORIDE (PROBE)
STANDARD PLATE COUNT
TOTAL DISSOLVED SOLIDS
TOTAL ALKALINITY (PH 4.5)
TOTAL COLIFORM
TOTAL HARDNESS
TOTAL RESIDUAL CHLORINE
TOTAL SOLIDS
TOTAL SUSPENDED SOLIDS
<5 MG/L 500 UMHOS/CM
17 COL/ML 280 MG/L 220 MG/L <1 COL/IOML 240 MG/L <0.02 MG/L 280 MG/L <1 MG/L
* = CONTRACT LAB
5
1 1
SUBMITTED BY
DATED
002118
