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: AR226- 0509 ACUTE TOXICITY TO FISH TEST SUBSTANCE Identity: P26e,rfolruoFrXo-o1c0t0a1no.ic(Oaccitda;nomiacyacailds,o pbeentraedfeecrarfeldutooroa-s, PCFAOSA,# F3C35-67-1) Remarks: The 3M production lot number was not noted. The test sample is FX-1001. It's purity was not sufficiently characterized, though current information indicates it is a mixtureof 85 - 98% test substance and 1 - 5% perfluorochemical inerts. METHOD: Method: Not noted. Type: Static acute GLP: No Year completed: 1985 Species: Pimephales promelas Supplier: Dale Fattig of Brady, Nebraska. Analytical monitoring: Temperature, pH and DO Exposure period: 96-hours `Statistical methods: Probit analysis Test fish age: Not noted. Length and weight: Average length = 4.1 cm Average weight = 0.50 g Pretreatment: 25.0 mg/L. tetracycline HCI, 5 months prior to study initiation. Test conditions: Dilution water: Carbon-filtered well water. Dilution water chemistry at test initiation: "Temperature: 19C Dissolved Oxygen: 9.5 ppm pH: 7.8 Stock and test solution preparation: A stock solution was prepared by dissolving 45 g test substance (neutralized with NaOH to pH 7.5) in3 liters of water and transferring appropriate aliquots to test vessels to make 5 liters of test solution at the selected test concentrations. Concentrations dosing rate: Once `Stability of the test chemical solutions: Not noted Exposure vessels: 24 cm inside diameter and 26 cm deep glass beakers containing 5 liters test solution. Number of replicates: two Number of fish per replicate: 5 03849 Loading Number orfatceo:n0c.e5n0trg/aLtions: five plus a blank control Water Dchiesmsiosltvreyd odxuryignegntrhaensgteud(y2:4 --96 hours): 67.13--76.77 mmgg/LL ((c8on7t0romlge/xLpeoxspuorseu)re)" pH ran7g.e5(7.2-7946(cohnotruorls)e:xposure) + 870 m7g5l5L7t.es5t g(r8o7u0pm(gs/eLcoenxdpohsiugrhee)st concentration) data given because total mortality occurred in the highest test concentration by T4e8shtoutresm.perature (2-4 96 hours): 19 - 20C RESULTS Nominal Element vcaolnucee:nt9r6a-thioounrs:LCB5k0c=ont8r4o3l,(689101,-785708,)8m1g0/,L.870, and 930 mg/L. Element value based on nominal concentrations. CONCLUSIONS The 843 test sample mg/L with a 8965-%hoCuornLfiCdsenfcoer fIanttehrevaadl mofin81n1owtow8a7s8dmegt/eLr.mined to be Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY tReelsitaibnigl.ityH:owKelviemris,chsarmapnlkeinpgur2i.tyTwesatsinngotmeperotpsertlhye criteriafor quality characterized and it lacks analytical confirmationoftest substance concentrations. REFERENCES This study was Laboratory, St. conducted Paul, MN, LbaybtRheeq3uMesCtoNmupmanbye,r CEn1v0i0r6o,nmceonmtpalleted on February 2, 1985. OTHER Last changed: 5124/00 C03850 concn= , coon coma. a0 se flo 10 xo ------ <5 1o0urr1a.mesnovuossowroseosur_S sroxiererg orA[Xf4r 0wr)ois acobmmlonse') Fe eRCERe FRALEY 4-8. wm _ em. _ 10 10 \o 10 =0 50 10 Ho ue "0 co 160 a0 sem. 10 0 50 106 ; ] J 150 (O55 CONrIoam CET05 plaleee) eleva) | e.ds(elEi RSTue8) rn rps gree re cid sie pei rp ses FMS ly): 2 (0 er te donfresponsc. Tonsil [30% Morlaity: a2 Rw ie saou ecnommiPtic tiarasSseienshBwira,LATs L NGe) sBwenf \2-B5Ue) wg W5e gem, s hog. sahDiseaseTreatmme: Siz/ecm,No,Geganiemsperdose, Loading 250 snl) isleAoneVY Factor 25 7,50) 4 9/1 test:SolutionVolume,S_ 1it&F#/ALL-Glass Terk Tank Dimensions: She aa sioe started_o5/{77m ove tarts Lf fs patecompleted 2//2 JAS Jilution WaterCarbon filtaredwellwater (fap, _/G C. 0.0. ZS pm w_ZH8 iS ci (95% Confidenceunion BL(512-3 hell. eut1 FEE EPEe Pe re Tee [os Twsolasol 0 boll 0 Wed] 0 dul 0 su] Slamlanl #4 bse abeled 5 HET 5 1 ti rrrJr 1 E cat. s Temp, t tr =oTo. |I= rr 111 11] 20T1 R =7 Se2|n2 S72 77 7)| sch: DAE J--p Es Ly 5 ed =Lg illus itt 2 IW; ? TE aad aid had) No. Symptoms or Reactions 24-Hr. 48-Hr. T2-Hr. 96-Hr, m" agT 1 1 A ZZ ZT rr | ST ITard] lee TT I> le 1 INC TY pv 7 | =e 111 coasnzl er pf Visto ULadel AQIATIC TOKICITY DATA SHEE LR A 773 DEFIN:TIVE EHS ORE TEST) yooTest nilXitrESHE ovesial Teste estornSY Arir nto.so Fn 5 Lot: ateRect.aZag batchWL n S) wg.We. JSD)arems vg. Size,[om vo. Open msperdose_.__losding Factor_J, 7) 9/1 sat.SolutionVolume, Litars/ALL-GlasrksTankDisersicns: `55538 DIES ger imeStarted HQuPees Started (59 [5:5 vn cmeiems 2[2 [BS FE EETE PETe Th "lutionWaterCarbon-filteredwellwater (Temp, [9 C.,0.0. 2, = 165, (95% Confidenceun Pull sme) = Spm, BH 7 J ISL Jugal 4SS Tlet 2 blza] 0 tts] 0 Wis 0 dz] llama]+ ded 2. bidps]20bids]5 bias] oxlzol0 duds baw] 5 = .s =] erir rr rrr r rtd tr Tr 111 111 R Test e Tem, t 1 ZSp f c 20 Ee| 72 a | 727% | Spl TIT RRO eR 0 SH 220 Ti a] NT LT Oud.FE J WdT iaSsRACcAadTiIrdaza TaTd 7. old ualh Bal Icc on Ue = ne _ sew, mr AA Z] Ta 71 1 7| cilagm 17 De TWINS ga Vp pr] ee = [= 1 ---- ---- |] 1 1 toes]