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AR 886 -- Of 40 ACTIVATED SLUDGE RESPIRATION INHIBITION TEST TEST SUBSTANCE Identity: AremfiexrteudretocaonstPaiFnOiSng,pFeCr-f9l5uo,roorocatsanaescuolmfponoanteen,twhoifcFhX-m80a1y. al(s1o- be Octanesulfonic acid) (CAS # 2795-39-3). Ri1se2Lm%-a9rd2ik3es5t:h(yFlXe-nT6eh01ge)l.y3cMoClpubrruortdeyunlctteitinhoefnro,lrom8ta1tn.iuo2mn5bi%enrwdaiwtcaeatrse,so2ct%8is3p5ar-o3mpi4ax.ntueTrhSeeuoltfteo1snt%esPaFmpOlSe, fcohiaomreora,ly1l9)% hseoxdaimuimniocutmylchsluolrfiatdee,, 10.%8s%oxdainutmhadnecgyulms,ui0fa.t8e,% 0.1% N-(3starch, and 0.05% tolyltriazole. - Tchhaerafcotlleorwiiznegd scounmcmeantrryataipopnlsieosftiompaurmiitxiteusr.e Dwaittha imnacyomnpoltetaeclcyurately reflect the toxicityofthe fluorochemical componentof the test sample. METHOD Method: Activated sludge protocol EARMAS 5-09-84 TGeLsPt:tyNpoe: Aerobic YASnteaaatlriystCtioiccmaaplllmmeoetnteihdto:odrsi1:n9g8:4ResDuilstssolwveerdeodxeytgeernmicnonecdenbtyractailocnusl.ationofthe oxygen `TWcoaossnttseuowmrapgttaienorinTsrrmaetsae.otumrecnet:PlaAnctt,ivSatt.ePdausll,udMgNe mixedliquorfrom the Metropolitan Test condition: DSRiyelnfutethrieeotnnicwceastaeenrwd:agtDeees:itosNnooiltzuegtdiivoweannt.perre.paration: The test material was added directly to the dilution water on a weightvolume basis. TNeusmtbveersoseflsc:oncNeonttgriavteino.ns: three plus blank control, in duplicate (two sTEtolutedamileessnutrsubnpa)esnisd:edRessopliirdastiaonndinhpiHbitoinondaasydoeftetersmtiinnegd: bNyootxgyigveenn. consumption. RESULTS Nominal concentrations: Blank control, 10, 100, and 1000 mg/L. 002100 wEalsesmeeenntavatlu1e0s0:0mg/NLowiinthhibiatcoryoefnfecttimoaencoacftt7ivamtiendutselsu.dge respiration rate Element values based on nominal concentrations. TReesmtainrgkswafiselcd.onTdhuectveadluoensarempioxrttuerdeaapspldyestcortihbaetdmiinxtthureeTaesntd Snuobtstthaence fluorochemical proportion alone. CONCLUSIONS Texhpeosmuatreer.iaTlheexhriebsiptierdantiooninrhaitbeoitfotryheeffteecsttsbaitor1e0ac0t0ormgw/aL.sohviegrhear7thmainnuthtaet of the blank controls. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY uResleida.bilTihtey:meKitihmoidscwharsannkoitnagn=a3g.enTchye-atepsptrloavcekds mreetchoroddo.fmTehtehiondoocluolguym is `nsoatmpprloepearnldy tchhearsatcutdeyrilzaecdk.s aTnhaelryteiciaslacloancfkiromfacthiaornacotfetrhiezaatmioonuonfttohfe test fluorochemical proportion in the solution. REFERENCES -- se TSth.isPasutlu,dyMNw,asLacbonRdeuqcuteesdtbnyutmhbee3rMB1C6o8m4p,an19y8,4.Environmental Laboratory, ower Last changed: 6/26/00 002101 Acute Toxicity of a Com3pMouEnndvir(oSnumbesnttaanlceL)abtoor.aAtcotriyvated Protocol Reference: EARA/AS Sludge Mixed 5.09.84 Liquor Ev wb rucunst mos TUL SSA wwtns Lo fF woatyrer TS. os, oni CER . Totractivated sludge mixed, Iquor " collected from the St. Paul/Minneapol i's senole description L-223 [EXLY) opBITS saoCFaRlCPan( Masei rwars SR T ST eh peei?h We(4) dilutead itnom eesc. {OA a ompeOs) LaloolisTou [57] 4a] =loolselasusla[se] [fis] [ou] Utlool571g Leclno|4/ | [mua y l[ua ae] [gal ys [elool=[5m ala ipeazia sl 5a] [5d] 2512|0au)s] f2e10p0 |i2a/t14i9o|gQ|rT l|za20l]|{L2 ieoss] oledle lT o m Tod 1/5] LLL[TT *COIMMoENuTgS:ious. WweGHts ey o 002, (60 looo 0.05 200, 3. 1TT] BEST COPY AVAILABLE TR isthe Brent Consumptionvate Grau dpi) : Acute Toulclty of a Com3pKouEnndvir(oSnumbesnttaancle)Labtoar-aAtcotriyvated SludgeHixed Liquor i eb rounse non: Protocol Reference: papas 6.09.86_~ vt LBA] owe: o/20S/L stress, Ss MTehteroacWtaisvtaetweadtesrluPdlgaentmioxnedL11guI gr EwesDcol:jected from the St. Paul/Minneapolis Semple descriptions _ = FZE [EHpp/, Jor 23 SS Fp er oti mre, CER Geshe Festwater OTE ST nat on? re Sted to m1 Srkl | 10g (0) 100g tonn Rent? Geelh L2.100z [529lb []s5.0[o #g 9 o l uyall d ade ]] s[|ops uod]] Caloolu[sy[yn Lsladse] z= da||u| 2| a Two) Fefasac lz eae] 0 olz.02| % P29]i Fapetiatiol 1 Tz 1] LdeolTo T 1l5osool] LLL1 [TT 1 Felis) veins BEST copyAVAILABLE lo 2.00%: \00 30.05 looo 3003 "Rois te Ogercousunplion vetealu al win). . SampleDesrpion 7258 Epon) FussFan" . Environmental Lab -- WorkSheet Sige TOXELY AACkATINS LabRocueiNB o LEL SL = PWt--L--ZoZt owe_tpf22/RF PI iy ] -- R=Oyugeacomsumplionve,(woOnffauduan) _ SeYest - eub 0000s 0000t 000e 0 wee _C:=CobedA 0000000000000 _Co=Caede\z epee OLComdeotd ~~ _locowgll Conde 2) O00 -- me 0oMas 0 sl -- iomICop=>\000mgd To ntterencewm Controls= LOR op J --R2avReo : - - T = h 25s x 100 0 0=22% 000 oozes fom toaz08.8 m0 rn . piccisds SUSom ) % [nti lot --_-- so come Za Z) --Skgase VOKILIT ou, Bisou sik roo ~ =(27 Ts 4 0c we 000000000 fod wal] | xv ole CoM TCge = > ocowgl) ol ttteerece t1aCQuilcheuskol=- Cerreetse, 0D = | BEST COPY AVAILABLE = = 20 100 = 2,.%%h - 002105