Document kDZEa1byEzEGmwYDd4bdo4JYb

M aq.6 _ 0 7 2 7 ACUTE TOXICITY TO AQUATIC INVERTEBRATES (DAPHNIA MAGNA) TEST SUBSTANCE____________________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-206 or L3243. (1-Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was not noted. The test sample is FC-206, identified by the laboratory as "Sample C". Current information indicates it is a mixture of 0.67% PFOS, 17.5% diethylene glycol butyl ether, 78.91% water, 1.33% Sultone foamer, 1% sodium octyl sulfate, 0.04% sodium lauryl sulfate, 0.5% polyoxyethylene monooctylphenyl ether, and 0.05% benzotriazole. The following summary applies to a mixture with incompletely characterized concentrations of impurities. Data may not accurately reflect toxicity of the fluorochemical component of the test sample. METHOD:____________________________________________________ Method: Methods for Toxicity Tests with Aquatic Organisms as formulated by the Committee for Toxicity Tests with Aquatic Organisms Type: Acute static GLP: No Year completed: 1974 Species: Daphnia magna Supplier: In-house cultures maintained at Bionomics, EG&G, Inc., Wareham, MA. Analytical monitoring: Not given. Exposure period: 48-hours Test organism age: 12 - 24 hours old Statistical method: TL5o (median tolerance limit) values calculated using a linear regression equation. Test conditions: Dilution water: Well water, aged 24 hours Dilution water chemistry: Not given Lighting: Not given Stock and test solution preparation: Not given. Exposure vessels: 250 mL beakers with 200 mL exposure solution Number of replicates: 4 Number of daphnids per replicate: 5 Number of concentrations: five plus a blank control Food: Food, consisting of a homogenization of starter trout food and Cerophyl in water, was added at rate of 0.5 ml_/L after introduction of test substance. Water chemistry during the study: 001507 Temperature range (0-48 hours): 20 1 C RESULTS___________________________________________________ Nominal concentrations: Blank control, 3200, 4200, 5600, 7500, and 10000 mg/L. Element values: 48-hour TL50 = 5850 (4810 - 7110) mg/L 48-hour NOEL = 3200 mg/L Element values based on nominal concentrations. Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS_______________________________________________ The FC-206 48-hour TL50 was determined to be 5850 mg/L with a 95% confidence interval of 4810 to 7110 mg/L. The 48-hour no observed effect level (NOEL) was 3200 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY_______________________________________________ Reliability: Klimisch ranking = 2. This study meets the criteria for quality testing. However, the sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. REFERENCES________________________________________________ Test was conducted by Bionomics, EG&G, Inc., of Wareham, MA at the request of the 3M Company, St. Paul, MN, 1974. OTHER______________________________________________________ Last changed: 6/28/00. 001508 "y BIOASSAY REPORT SUBMITTED TO 3M COMPANY . S T . PAUL, MINNESOTA ) v i? ACUTE TOXICITY OF SAMPLE C TO WATER FLEA (Daphnia magna), AND SCUD (Gammarus fasciatus). Bionomics E G & G r Inc. t Environmental Consultants --y 790 Main Street Wareham, Massachusetts August, 197^ 001509 METHODS AND MATERIALS These investigations were performed at the aquatic toxicology laboratory of Bionomics, E G & G, Inc., Wareham, Massachusetts. The susceptibility of water flea (Daphnia magna), and scud (Gammarus fasciatus) to Sample C, a brown liquid, v/as evaluated under static bioassay conditions for a J-8-hour period. Results were expressed as the median tolerance limit (TL^Q ), the nominal concentration of the test compound in. water causing 50 percent immobilization for the Daphnia and Gammarus. The TL^Q -value and its 95$ confidence intervals were calculated by converting the test concentrations and the corresponding observed percent mortalities to logs and probits, respectively. These values were then used to calculate a linear regression equation. Procedures for static bioassays as described in the Methods for Toxicity Tests.with Aquatic Organisms as formulated by the Committee for Toxicity Tests with Aquatic Organisms were followed, except for those conditions described below. The Daphnia, and Gammarus were acquired from laboratory cultures. The Daphnia and Gammarus bioassays were conducted at 20C (j+ 1.0). The invertebrate tests were conducted in 001510 Page two 250 ml beakers containing approximately 200 ml of well water which was aged for 24 hours. Five 12-24 hour old Daphnia. and five .fourth instar Gammarus were placed in each beaker (4 replicates/concentration). In the i Daphnia bioassays, Daphnia food was added at a rate of 0.5 ml/liter after the introduction of the compound into the test vessels. RESULTS The predicted TL^q values and 95% confidence intervals for the Daphnia and Gdmmarus are presented in Table 1. Table 2 presents a summary of observed immobilization for each individual test after 2k and 48 hours. ^Food was prepared by homogenizing 5 g of starter trout food and 1 g of cerophyl in 100 ml of water. 001511 SUBMITTED BY: Bionomics E G & G, Inc. Environmental Consultants 790 Main Street Wareham, Massachusetts August, 197^ PREPARED BY: Robert E. Bentley Je- Chief, Acute Toxicxtp Investigations f APPROVED BY: Bevier Hasbrouck Sleight, III Dirreeccttoorr., Freshwater Programs V 001512 Table 1 -- Acute toxicity of Sample C to Daphnia3 and Gamtnarus.k The data are based on results of bioassays conducted at the fish toxicology laboratory of Bionomics, E G & G, Inc., Wareham, Massachusetts. Species TL^Q-milligram active ingredient/liter 24- hour 48 hour No Effect Level (mg/1) Daphnia Gammarus > ! o /0 5.310(4,340-6,510) 5,850(4,810-7,110) 3,200 5.170(4,190-6,370) 3.200 aBioassay conducted at 20C (_+ 1.0), Daphnia 12-24 hours old at the initiation of tests. Bioassays conducted at 20C (* 1.0) Gammarus 12-24 hours old at the initiation of tests. c95# confidence intervals. 001513 Table 2 -- Concentrations tested and observed percent immobilization for Daphnia and Gammarus exposed to Sample C after 24 and 48 hours. Species Daphnia Gammarus Concentration (mg/l) 10,000 7 ,500 5,600 4, 200 3,200 control 10,000 . .7,500 5,6oo 4,200 -3,200 control % immobilization observe 24 hour 48 hoi 20 100 25 50 0 40 0 10 00 00 oo o 100 100 V 80 6 75 45 50 00 00 001514