Document kDGka9KYedDB4xD5xqLwnkdMn
UNION
Rdiiitgd
BUSHY RUN RESEARCH CENTER
FO. +. Mallon Road, Export, Pennsylvania 15632
Telephone (412) 733.5200
PROJECTREPORT. 47-527 SRE
TITLE: T-3607 Acute Inhalation Toxicity Test
AUTHOR: Donald J. Nachreiner
DEC 27 ies:
Zp,Hien
SPONSOR: 33M1 CCoemnptaenry 2St2.0-2Pa8u-l0,2 NN 55144
INITIATOR: William C. MeCormick
DATE: December 19, 1984
000062
A Joint Mallon insutshuhtya URnuinoRneseCaarrchdsCoGnotreproration Operation
UNION
CRU
BUSHY
H75. orFonn e, Exper,
RUN RESEARCH CENTER
Pemwmoo aranaTeas0eTrems
P11roPjaegcetsReport 47-527
December 19, 1984
Acute Inhalat2f1o-n360T7oxtetty Study Sponsor: 3M Company
rans
Abstract
Five male and five female Wistar albino rats vere exposed for four hours to
aTtPhhoeevsratepepooxwsrpetoreosexfuprnoeTos-u3prm6ee0or7ri.topaedrsli.iTothdie.eMsaecaTtonhrueabrlcoeldiycwnheiwarcemeaiblgenhrotsicggognransoicsnesonfwtarpsaaftothxioolobelnsioetgwryiaveseddul3er.fsi7oin4rognmbsgos.h/tehL
es(xe1pv8oe2srnpcdpeum)r.oirng The revise
of this study indicate that the four-hour LCSO for 1-3607 is greater than 3:74
mg/L.
Objective resulTthiinsgsftruodny waassindgelsei,gnfeodurt-ohoduerteerxnpionseurtehetoac1u-t9e60i7n.halation toxicity to rats
Materials and Methods
StandTahrids asnteunddyaefnotlltoowetdhethperostpoeccoilfipcreppraorteodcolby (tBhReRCBuPsrhoyjeRcutn 8R4e-s6e2a-r4c0h122C)entaenrd. Test Article
1(1St6..
APatuwlo,-q1ua)r,t absostitglneedofBRTA-C36S07abwiaes Infornation received fron the
Nruemcbeeirve4d7-o2n09A,uguansdt Sponsor stared the
s9t,or1e9d841afrSoemca3sM purity to be
SC0o5spaanndy
aRpepgriosxttrayatNeulsybe1r00v%aspneortfluaovreoiolcatbyile sfurlofnontyhle fSlpuoonrsiodre:. An sdestisitaton or CAS
A doit Mallon 0IS0nu0sty0Fr6un3oaRnoGaorceCaGnoevroraton Opin
Antnal Spectes, Source and Husbandry
RPaegpeort2 47-527
oaLnanbdSeMAfnpaeitlmmeeaamllbaesen,rdfa1tIf9sne,cm.aw,1le9er8eS4cH,oi4t5latlndtadanoldpea-,sW5si9isPgAtd)naaeyrdwseaurolnefbiiquanusgoeeed,r.iadtresensOtnp[ie2fct0ith0cei-av3ted0ila0oyyn.g,ofauTHsehLbxeAepyCroWswsIeu)rbrByeeR]trhe(ecHeimilavlletedop stPtohaeoi~tncollpeiesprspiionsdgt.etehlroTwuhigerheoruamttessthwheercaepgoeshstoueosxnepdoscuaFrriervieeprepsreiroidns.eRxoAoimnl12a03y9:e5ranxodf4Kb0ee.po0ttxivoen1d5&.A01n3ci-amhahouirgh Cage Board (Shepherd Specialty Papers, Inc., Kalamazoo, MI) vas placed WeuCxehnpsodowte,saruorrAeeega.lvcaahnydrIonwCco.uo,nftySc,yargeaGscr.ueseen,sPbeuNlrYl)ge,taendPdA)fteaweepdrwe(aAtagevwraaiyl(aHPburlnoeileaaibdpaRl1MiHAb3ui0tt0hu0osrCieteryxtcioeffpietddRuordienngt
Inhalation Chamber Design and Operational Characteristics The rats were individually housed in 16.5 x 9.5 x 15.0 ca wire mesh cages
aao1nppadperrRoeaoxxtoipimmooasn1te1ea8d.lryeinT2sa5outml1ami2lat0re-iarlzisiertdfpeleriornw(mTatiaphnbprulroteouexg.i1h.matTthheeevcoihlnauhmmabele)artwicaousnboicmdhaaailmnbteParliendxeeidsgilagasnsancdhamber Exposure Regizen
SepteFmibveera2l4e, 1a9n8d4,fitvoe were perforned.
afevmaaploer raattmsoswpehreereexopfosTe-d36o0n7c.e
fNoor
cfoonutrrohlouerxspoosnures
Generation of the Test Material
The 17-3607 vas metered with a piston pusp (FHI Model REG-6/Lab Pump Jr. Aesvsaepmobrlayt)ioinnatto aamgbliaesnst ttuebmepecraotnutraei.ningThgelafslsuibdeavdass umseetderetdo faatcailirtaattee that waectovoruamflppldororewapstrsoie(vodainpdapeirtroubaxewi.snaaostmeiuFlnisyageludr2e5ctoonl1civteapenprrtoesrrsaietpznieetorsntmahioenfsucttaheepse)ptmrawomtaxaisitcmeaprdtaiiesaalslgye,rda5mtThmohgrfe/oLuet.ghnhetiDtrgrheeyen,cehrfaaimtlbiteoernreadn,d exposure systea.
fLilteeerArslexdowfathwsartoreuergm.hovtehdreferosmcrtuhbebicnhgasdbeevricveisa eaacvhaccuounntallinnei.ng aTphperoexxihmaautsetlyaiwr owas
Target Concentration
guideTlhienetsarfgoert c"olnacietn"trtaetsitoinngfosretthfiosrtehxpobysurtehe waTsoxi5c mSgu/Lb,stabnacseesd Coonnttrhoel Act
(1sca).
000064
Report 47-527 Page 3
Chamber Concentration Analysis
everyThe30 cmoinncuetnetsr.atiAonknoofwnT-v3o6l0u7meinofthaeirchwaamsbesramwpalsedmefarsoumretdheapbprreoaxtihmiantgelzyone
of the flasks
animals through evacuated containing the atmosphere
flasks supplied by the samples of T-3607 were
3M Company. sent to the
The Sponsor
for analysis of perflurooctyl sulfonyl fluoride.
the Ttheestnommaitnearlialconucseendtrbaytitohne wtaostadletveorlmuimneedofbyaidrivwihdiicnhgpathsesetdottahlrowuegihghttheof chamber during the exposure period.
Temperature and Relative Humidity
The temperature and relative humidity in the recorded continuously with a seven-day recording
animal housing room were hygrothermograph (Cole Parmer
Instruments, Chicago, IL). with a Series 400 A Digital
TDruernidnigcatthoerex(pDoorsiucreSctiheenttiefmipce,ratSuanreDwiaesgo,moCnAi)toarnedd
the humidity was monitored Inc., Oakland, CA).
with
a
Model
8501
A
Humidity
Module
(Hygrometrix
Animal Observation
exposAulrle afnoirmaslisgnwseroef otbosxeircveedffdeucrtisn.g exposure, and for 14 days following
Body Weights
and
The animals were weighed fourteen. The change in
bpordiyorwetioghetxpwoassurcealacnudlaotnedpobsytesxupbotsruarcetidnagysthseeven
pre-exposure values from each successive weight.
Necropsy
The aninals anesthesia, the
were sacrificed on October animals were exsanguinated
8, by
1984. Following methoxyflurane severing the brachial blood
vessels. A complete necropsy was performed. No tissues were saved.
Statistical Procedures
and
The mean exposure
aconndcesnttarnadtairodnsd,evwiearteioncaslcouflattheed.bodNyowesitgahttiss,ticbaoldycwomepiagrhitsocnhsanwgeerse,
made.
Storage of Records
BushyThReunfiRneaslearrecphorCtenatnedr.all raw data are retained in the Archives of the
000065
PRaegpeor4t 47-527
Chamber Concentration
Results
sulfoTnhyelmfelaunor(i+desta(nTd-a3r6d07diesviaatpiporno)ximmaetaesluyred100c%oncPOeSnEt)ratiinontheofexppeorsfulrueorocohcatmybler wcaosnce3.n7t4rat(io0n.s26r)ecmegi/vLe.d fTrhoem itnhdeivSipdounaslordaatrae fporrestehneteadctiunalTaebxlpeos2u.reThe nominal concentration was 5.2 mg/L.
Hunidity and Exposure Chamber Conditions
The betveen
2t1emapnedra2t2urCe
and and
4r3elaantdive57%hurmeisdpietcytiivneltyh,e
tahnriomuaglhohuotusithneg
srtouodmyrpaenrgieodd.
Tchheammbeeranwa(s* s2t6an(dar0d)Cdeavnidati1o6n)(*te5)m%p,erarteusrpeectainvdelhyu.nidity in the exposure
Antnal Observations
durinTghetrheew1e4r-edanyo pcolsitneixcpaolsusriegnsperoifodt.oxicity observed during the exposure or
Mortality
No rats died during exposure or during the l4~day postexposure period. Body Weights
TableThe3. inMdeiavnidbuoadlybwoediyghwteigghaitnssawnedrebodobysewreviegdhtfocrhabnogtehs days 7 and 14.
saerxeessuomnmaproiszetdexpionsure
Necropsy
No gross pathologic lesions were found in any animals.
Discussion
mg/LATs-i3n6g0l7epfrooudru-cheodurnoexmpoorstaulrietyt.o anThaecrteuawlerechnaombecrlicnoinccaelntsriagtnisonobosferv3e.d74 wdueriignhgt edxuproisnugretheorpdousrtienxgpostuhreel4p~edraiyod.postTehxeproesuwreerepenroiogdr.ossBoptahthsoelxoegsicgained lWeissitoanrs.albiThneo rreastusltiss ogfreathtiesr stthuadny3i.n7d4icnagt/Le.that the LC50 for T-3607 vapor in
000066
Project Tean
WD. LJ. B. K.
SNtaecehlretaez, B.S. Kiome, Ph.D.
Reviewed and Approved by:
T1564 WPC/rkk/0630B-2
RFeaptoer'ts 47-527
SPSertnoutjdoeyrctDiTMeracenhcaatgcoelrroptst
Dovad Y Yuhnsonan 13-021
Da SotmuadTyc dDiJrh ectorre3t5er, Tate
TPDerononjiersctaR,HanKR aigoewre,W Ph:
1Lo
Fred R.
Sete
Frank, Fob.
Director
pp -Dxate
Alep
tate
000067
Report 47-527 Page 6
Table 1
Inhalation Chamber Design and Operation 1-3607 Acute Inhalation Toxicity Test
----------- CHAMBER
Location: Room 118 Construction: The chamber was made of Plexiglas. Shape: Cuboidal
Dimensions: Height - 0.38 meter
WLiedntghth--
0.61 0.52
meter meter
Total Volume - Approximately 120 liters
Alrflow Measurement:
AdrMyantoessttatmetfelro,wmewtaesr,poscailtiibornaetdedinwitthhe
a Singer air supply
line to the evaporator tube.
Test Article Generation: eFvMaIpoMroadteolrRtPuGb-e6/cLoanbtaPiunmipngJr.glaAssssebmebaldys;; galmabsisent temperature.
Chanber Atmosphere Conditions:
Chamber Number
Nominal Concentration
(mg/L)
120-1
5.2
Temperature cc)
Mean * SD 26+0
Relative Humidity**
@) Mean + SD
ts
Afr Flowrate (L/min)
25
--_--
* Deternined **Deternined
using using
a a
Series 400 Model 8501
A A
Digital Trendicator. Humidity Module.
------
WRC/rkk/06308-2 11-19-84
00068
Report Page 7
47-527
Table 2
Exposure Concentration Measurements _--mm---- 1-3607 Acute Inhalation Toxicity Test Actual Concentrations:
Sample Number
Time into Exposure (min)
Sample Flowrate L/min)
Sample Duration
(atn)
POSF* Concentration
(ng/L)
(pp).
a
55
B
65
c
1s
>
125
E
175
F
185
210
H
25
1
5
3.45
168
1
5
3.47
169
1
5
3.65
178
1
5
3.48
169
1
5
3.94
192
1
5
3.87
188
1
5
3.99
194
1
5
4.11
200
Nominal Concentration:
Standard
Mean Deviation
3.74 0.26
182 13
Target Concentration
(mg/L) 5.0
Exposure Duration (ain)
240
Chamber Adrflow
(L/nin) 25
Total Mass of T-3607 Used (grams)
31%
Nominal Concentration
(mg/L) 5.2
_--
*
pTehrefl1u-o3r6o0o7ctsyalmpsluelsfowneyrle
analyzed fluoride
b(Ty-3t6h0e7 sipsonaspoprrofxoirmattheelyco1n0c0e%ntrPOaStEi)o.n
of
**
TA-3v6o0l7ume(17o.f21m7i.r2demlnsiTt-y36o0f7
was 1.8
metered. gu/nl) was
At ambient vaporized.
temperature, The density
31 grams was
of
deternined by weighing 10 ml of T-3607.
_--mnmmm----
WRC/rkk/06308-2 11-19-84
000069
Report 47-527 Page 8
T NMummibmearl
-_-- 84-14763 84-14764 84-14765 84-14766 84-14767
Table 3 1-3607AcIundtieviIdnuhaallaAtniiomnaTloxDiatcaity Test
_e Welght t At t Day Bod7y WE. C1h4ange
wMAeLEs S*
261 270 306 246 284 324 253 304 348 245 295 353 258 329 378
29 65 38 78 51 95 50 108 7 120
GroastsNPecarotphsyos NoL NGL NGL NGL NeL
Mean: Std. Dev.:
249 296 342 7 22 28
48 93 16 22
Mortality Ratio 0/5
Group Observations:
No clinical exposure or
signs were observed 14-day postexposure
during the periods.
4-hr
vapor
_--
FFEeMALnESs*
8844--1144882289
21 266 276 201 243 257
25 35 2 16
NL NOL
84-14830 84-14831
251 263 282 243 256 267
12 31 13 24
NGL NGL
84-14832
228 249 252
22
NGL
Mean: Std. Dev.:
261 255 267 8 10 13
15 26
9
7
RMaotritoali0t/y5
Group Observations:
No clinical exposure or
signs were observed 14-day postexposure
during the periods.
4-hr
vapor
-_--
NGL = No Gross Lesions
*ExAplobsiunroeraDtatse:[HLSAe(pWtIe)BmRb]erH2i4l,lt1o9p84Lab Animals, Inc., Scottdale, PA were used.
EEE
EE
----
WEC/rkk/06308-3 12-10-84
000070
|
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svoteer
Figure 1 TBGeeneirantionToatntdoBn estePs ne
Eiposue chaser
i [=]
Sicrubebing
e-ters
hr --
fWi]) cfiees
g) cotuen, fi I
= == ==
B
PtY est
Reservoir
Flowmeter
pros
Afr Source
i
PRaegpeor1t047-527
UNION
Kitab
BUSHY RUN RESEARCH CENTER
FO. . Mellon Road, Export, Pennsyivania 15632
Telephone (412) 733.5200
Conpliance with TSCA Good Laboratory Practices ContTrhoilsAcsttud(yTSwCaAs) cGoonodducLtaebdoriantoarcycoPrrdaacntciecewsitwhithcurtrheentfoTlolxoiwcinSguvbasrtiaantcieosn: 1. aTvhnoeduldSoptohbneersosrcthaapbrrloaevcitfdeoerrdisttthihecesdpuwureraritetiyonnootfofatvhtaehieltaesbstltuedym.aftoerrHtotawhleeveatrne,dstiitnnhdgeicfcaaotcmeipdloistityht.aitonAint
4dentification or CAS Registry Nusber was not available from the Sponsors influItenc1se tthhee orpeisnuilotns oofr ithnetesrtpurdeytadtiiroenctoofrtthhisatsttuhdeys.e factors did mot
'1W2P-C1/0r-k8k4/0500B-1
T SDteud5r.a 3y.aDiicdrecIthorHraBh.egS.il nanne 13-r 10a-t2,4e
000072
A dant Melon InBtuhsuhtys--RunnoRnessGaarrchdeCoGnatrerparaion Operation
:
Report 47-527
_R ___BE USHS Y RUNEAR CENC TERH Ln)
ad
BUSHY RUN RESEARCH
A. D. 4, Mellon Road, Export, Pennsylvania 15632
CENT R
Telephone (412) 733.5200
Qualtey Assurdnce Unit Seuy Tospection sumary
52083 Standard protocol
5-208) 52063
10086 Standard Protocol Amendsent
203-60
fre
Bie
Av Olid
Lhofes
Good Laboratory Practices/Quality Assurance
A a ton eTyTTCton 000073