Document kD4X616kYDV99L5jBYvee1aZb

ACUTE TOXICITY TO FISH TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonawthei,ch may alsobe referretdoas PFOS, FC-95,oras a component ofFC-203A. (1Octanesulfoniaccid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was not provided.The test sample isFC-203A. Currentinformatioinndicateistisa mixtureof1.34% PFOS, 25% diethyleneglycolbutylether,67.85% water,2.66% Sultone foamer,3% sodium octylsulfate0,.1% sodium laurylsulfatea,nd 0.05% tolyltriazole. The followingsummary appliesto a mixture with incompletely characterizedconcentrationsof impudties. Data may not accurately reftct the toxicityof the tluorochemicalcomponent of the testsample. METHOD: Method: Not given Type: Acutestatic GLP: No Year completed: 1975 Species: Pimephalespromelas Supplier:Not given. Analyticalmonitoring:Temperature,pH, and DO Exposure period: 96-hours Statisticamlethods: LC50 valuescalculatedusinggraphicinterpolation. Test fishage: Juvenile Length and weight: Average length 2 in. Average weight 1.5g (wet) Loading: Not given. Pretreatment:None Test conditions: Dilutionwater: Carbon-filterSetd.PaulCitywater,St.Paul,MN Dilutionwater chemistry:Not given. Lighting:Not given. Stock and testsolutionpreparation:Notgiven. Concentrationsdosing rate:Once Stabilitoyfthetestchemical solutions:Not noted Exposure vessels: Not given. Number of replicates:1 Number of fishper replicate:Not given. Number of concentrations:fiveplusa blankcontrol Water chemistryduringthestudy: pH range (0-96houm): 7.0- 7.0(controelxposure) 6.7- 6.9(750mg/L exposure)* Temperature range (0-96hours):69 - 70 *F Dissolvedoxygen range (0-96hours): 4.7- 5.6nig/L(controelxposure) 3.9- 4.8mg/L (750mg/L exposure)* 750 nig/L(secondhighestconcentratione)xposurevaluesused because thehighestconcentrationused resultedintotalmortalitayt72 hours. RESULTS Nominal concentrations: Bk control1,00,250, 500,750, and 1000 mg/L Element values: 96-hour LC50 = 300 mg/L Element valuesbased on nominalconcentrations Remarks: Testingwas conducted on the mixtureas describedintheTest Substance Remarks field.The valuesreportedapplytothatmixtureand not the fluorochemicaplroportionalone. CONCLUSIONS The testsample 96-hourLCm forfatheadminnow was determinedtobe 300 mg/L. Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking3. Testinglacksrecordofmethodology used. There isa lackofcharacterizatioofnthetestsample. Test solutionwsere not analyzedfortestsubstanceconcentrationasnd confirmabonoftheamount of fluorochemicaplroportioninthe solution. REFERENCES This studywas conducted by the 3M Company, EnvironmentalLaboratory, St.Paul,MN, Lab Request number 2563, 1975. OTHER Lastchanged: 6/26/00 740101 L -ENVIRONMENTALENCINEERINGLABOPATO NEATIC TOXICITY WORK SHEET Type Test 96 Hours Material Tested Test Organism Fath*ad Minnow. Date Started Avg. Neight 5@@ v Date Completed Time Started. Plotted LCSO Dilution Water Carbon Filt*red St. Paul City Water Avg. Size @2, 1-975 Conc. 24'hrs Survival_ pH pps 48 hrs D.0, Survival pH ppo 72 hrs D.0 Survival pH ppa % hrs D.0 Survival pli pps Control '7.015. ?o 5.51 /00 io-5.o loo 7.04I. 691 lx@ 7.0 5.0 1 7.01 00 7.0 .1-@ 6o., -7.a "9.5 .60 6.94ql5 ,506 q-0 7,52 Avera ...t, droents: 6 01lq3 0 4.C) LC so CA& on 41 .. 43 @-4 50