Document k9noGbrRgjXd2y23O83J04eLD

ACUTE TOXICITY TO FISH AR336-0 W TEST SUBSTANCE Identity: Perfluorooctanoic acid, ammonium salt; may also be referred to as PFOA ammonium salt, Ammonium perfluorooctanoate, PFO, FC116, FC-126, FC-169, orFC-143. (Octanoic acid, pentadecafluoro, ammonium salt, CAS # 3825-26-1) Remarks: The 3M production lot number was 83. The test sample is FC- 143. It's purity was not sufficiently characterized, though current information indicates it is a mixture of 96.5 -100% test substance and 0 - 3.5% C6, C7, and Cg perfluoro analogue compounds. METHOD: Method: Not noted. Type: Static acute GLP: No Year completed: 1978 Species: Bluegill sunfish (Lepomis machrochirus) Supplier: Not noted. Analytical monitoring: Temperature, pH and DO Exposure period: 96-hours Statistical methods: Not noted Test fish age: Not noted. Length and weight: Average length = 4 cm Average weight = 0.20 g Pretreatment: Not noted. Test conditions: Dilution water: Carbon-filtered well water. Dilution water chemistry: Temperature: 19C Dissolved Oxygen: 9.1 ppm pH: 7.9 Stock and test solution preparation: Not noted Concentrations dosing rate: Once Stability of the test chemical solutions: Not noted Exposure vessels: Tanks containing 16 liters test solution. Number of replicates: one Number offish per replicate: 20 Loading rate: 0.25 g/L Number of concentrations: five plus a blank control Water chemistry during the study: Dissolved oxygen range (24 - 96 hours): 5.3 - 6.9 mg/L (control exposure) 5.1 - 7.3 mg/L (420 mg/L exposure) pH range (24 - 96 hours): 03741 7.9 (control exposure) 7.8 - 8.0 (420 mg/L exposure) Test temperature (24 - 96 hours): 18-19C RESULTS Nominal concentrations: Bk control, 135, 180, 240, 320, and 420 mg/L Element value: 96-hour LC50 = >420 mg/L Element value based on nominal concentrations CONCLUSIONS The test sample 96-hour LC5ofor bluegill sunfish was determined to be >420 mg/L, the highest concentration tested. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Klimisch ranking 3. Testing lacks information on the method followed. No information on stock or test solution preparations. Average fish weight is suspect. Sample purity was not properly characterized and it lacks analytical confirmation of test substance concentrations. REFERENCES This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number 3844. OTHER Last changed: 5/24/00 GQ3742 I. :.t * r' \ i: !.. . 3344 ENVIRONMENTAL ENGINEERING LABORATORY SHEET best copy availABLEAQUATIC TOXICITY DATA p. m Type Test ______ _________ M a t e r ia l T e s te d p 'C j , ~ 3 l^cA ^ 3 T e s t O rganisnT\jAwe.<?sJx^ ._____________ A vg. Wt.Q.*V^ grains Avg. S iz e %V cm T e s t S o lu tio n Volume J (_ & L ite r s / T a n k No. Organism s p e r dose X Q __________ Time S t a r te d "3 * 0 0 p W s D ate S t a r t e d H --H - ^ *7 Da te C om pleted D ilu t io n Water Carbon F i l t e r - W e ll W ater (Tem p.)0! C , , D.O i^ f | ppm, pH *1, ^ ) In v e s t ig a t o r \ _ . p . ________ LC 50 ( 95% C o n fid e n ce L im its ) - t *6 Cone. No. Irga nism s 24 H rs . D.O. Exposed No.Dead pH ppm C o n tro ' - ] 3dT `X O X <3 i^ O xo VAO 3X 0 Xo o _ , _ V O 0 <30 7 . 0 G *1.V .......,0 .. M ^O SrO 7 . 3 48 H rs . D.O. No.Dead pH ppm o X \ 6.7 o V e L,V o VC L X \ \ (.. o V G (p, U 0 V o U . 72 H rs . D.O. No.Dead pH ppm O 7-9 6.7 o 7.9 6/? 0 79 u go \ S3 \ 7.9 S ? l 96 H rs. D.O. No.Dead pH ppm 0 7.9 C.*? o 7 ? 4 o 7.9 0 t o S.2 1 0 1 n l Avg. Temp. Comments: /? C - / 3 C 'c . r* // /0 V__ \< f c . J C03743