Document k9Jw1MbVQGBLDdX0Qovj8yB8O
AR226-2770
FOR DD POUT USE ONLY
Haskell
E* I. du Font de NeB-ure and Co,, Inc.
laboratory for Toxicology and Industrial Elkton Road, P. 0. Box 50,
Newark, Delaware 19711
Medicine
HASKELL LABORATORY REPORT NO. 560-81
Material Tested
1) Octanoic add, pentadecafluoro-, ammonium salt*
Haskell No. 1) 12.037
14,008
Other Codes C-8
Study Initiated/Completed 6/2/B1 - 6/18-81
Material Submitted by
'CR&D Department' Haskell Laboratory
MOUSE FEEDING STUDY - 14 DAY
Procedure;
Ei.ihty male and 80 female albino Crl:CD-l mice (age 41 and 42 days, weighing 19 Co 30 g) were separated by body weight Into 5 groups according to
computerized randomization. The mice were housed individually and fed either C-ft (H "I/,037) orfa--------------------UHfA(H //14.008) mixed with Purina Lab Chow for 14 consecutive days. Concentrations tested were 10, 30, 100, 300, 1,000, 3,000, and 10,000 ppm (for each material). Separate male and
female controls were observed concurrently and fed only ground Purina Lab Chow. Individual body weights were recorded 3 times/week, food consumption
weekly-, clinical signs daily, and liver weights at the 14-day sacrifice.
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Company Sanitized. Does not contain TSCA CBi
Results; Female Mice
C-8 (H-12.037)
Concentration PP"
0
10 30 100 300 1000 3000 10.00C
[I
(H-14,0f
Concentration PPP'____
0
10 30
ion 300 1000 3000 10,000
Male Mice
Average Body Wts.
afcer feeding (g)
1 Week
2 Weeks
23.8 23.9 23.5 24.1 21.6 16.2 13.8 A.D.**
24.9 26.0 24.7 22.8 18.2 14.8
A.D. A.D.
------T]
Average Body Wts.
after feeding (g)
J Week
2 Weeks
23.5 23.4 23.7 23.P 2?.'' 23.''
21.7 1P.
25.1 24.5 25.3 24.7 21.9 25.1
24.1 20.0
Average Liver Weights (g)
1.53 3.02 3.24 3.94 3.39 2.27
'
-
Average Liver Weights (g)
1.55 1.83 1.992.09 2.94 3.26 3.14
... 56
Average Liver Wt/ Body Wt Ratio
(g/100 g body wt)
6.14 11.62 13.11 17.28 18.63 15.34
Average Liver Wt/ Body Wt Ratio
(g/100 g body vt)
6.18 7.47 7.87 8.46 13.42 12.99 13.03 12.80
C-R
(H-12.037)
Concentration
ppm
Average Bo.iy Wts.
after feeding (g)
1 Week
2 Weeks
Average Liver Weights (g)
Average Liver Wt/
Body Wt Ratio (g/100 g body wt)
0
10 30 100 300 1000 3000
10,000
27.1 28.9 28.1 26.4 25.8 19.1
A.D. 18.7
28.3 30.P 27.9 23.7 23.1 17.4
A.D. A.D.
1.69 4.13 4.40 4.48 4.10 2.48
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'
5.97 13.41 15.77 18.90 17.75 14.25
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Results (Cont'd.)
Concentr at ion
ppffl
Average Body Wts.
after f eeding (g)
1 Week
2 Weeks
Ave rage LI ver We ights (:g)
Average Liver We/ Body Wt Ratio
(g/100 g body wt)
0
10 30 100 300 1000 3000
10,000
28.9 27.7 28.5 27.2 28.2 27.1 25.2 22.7
29.7 29.3 28.7 27.5 30.3 28.8 27.4 23.6
1.77 2.75 2.36 2.38 3.41 3.03 3.25 2.87
5.96 9.39 8.22 8.65 11.25 10.52 11.86 12.16
Clinical Observations:
Concentreit ion
ppin 0
in
Observ.atlons
Weight loss.
Weight loss.
Stainpi' perinpal area, wclpht loss.
Weight loss.
30
Welpl'c Inss.
Weight loss.
ion
3 I'll'
inon 3000
Weakness, piloerection, sta incc1 perinf.il area, weiphc loss.
(Jnkenpt head arc.i,
weakness, tremors, uft and
stained .x-rineal area.
Death, unkeir.pt head area, weakness, trrmors, wet and stained perineal area, piloererfinn, wcipht loss.
Death, unkempt head ar-.-a,
weakness, piloerection, weight loss.
Weight loss. WeiRht loss. Weight loss.
Unkempt head area, weight loss.
10.000
Death, unkempt head area,
pallor, weight loss.
Unkempt head area, weakness, weight
loss.
SanlUx.-."------'""1"""60*08'
Company
__ Summary; Etcher C-8 o
were fed to aale nd
female Crl:CD-l Bice a 8 diffe_r_en_t__co_n_c_en_t_ra_ti_on_s_<| froo 10 ppa to 10,000
ppn) for 14 consecutive days. Death occurred at 1,000 ppa, 3,000 ppa and 10,000 pom In the C-8 mice. No deaths occurred in tl ""
Dice.
Body weight loss was indicated at the end of each week at 300 ppn, 1,000 ppm, 3,000 ppa or 10,000 ppm in C-8 male and female mice.
iJBii^^lB^UII^^^HlKr11^ 8howed a slightly lower rate of body
weight gain at 10,000 ppm in male and female mice. Liver weight/body weight
craotniotrsolsshoawnded sha owsehdarpa dInocsree-aressepoantse10Inpcpmreainsem. ^al--e --and--f--em--alBe HCB-8HHmHicHe Bo--ve--r tfht^e mice showed major increases in liver weight/body weight ratios at 300 ppm,
1,000 ppn, 3,000 ppm C-8 mice hadheavier
and
live
10
rs
,
00 at
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l
ppn
owe
r
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m
on
ales
cent
an
rat
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io
females ns than
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d
i
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and
----
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--
m
k
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e
--------------------B)11'1^*c-8 clinical signs included: weakness, tremors,
plloerectlon, pallor, stained perineal area, weight loss and unkempt ^
appearances from 100 ppm to 10,000 ppm. lIBBHHBHBBHIIBHBBBi^
clinical signs included: unkempt appearance weakness and weight loss at
3,000 ppm and 10,000 ppm.
In male and female mice C-8 produced greater liver weight changes and reduced body wei [htsatlower concentrations (from 10 ppm to 300 ppm) than
IB--------Rj Ac 1,000, 3,000 and 10,000 ppm C-8 produced
death anc
were observed in female and male mice from 100 ppra to 10,000 ppm of C-8,
whereas wl'chi
ew overt abnormalities were
exhibited.
* H ^12,037
Other Names: q_ C-8
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* H //14.008
Other Name:
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** A.D. -= All died
Report by:
^ '/^7,L.y..n.n >Si.y.F^o,r^d ti
Technician
Reviewed by:
0. Louis Dashlell Study Director
Approved by:
LSF;jrg:WP:i3.4
[ 1 Date Issued: October 26, 1981 Report No. .560-81
^.A4^ X^r^tJI. ^
Gerald L. Kennedy, ST. j Section Supervisor /
Acute Investigations
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3inpany Sanitized. Does no; contain TSCA CBI