Document k6zd4q7OnRBkLqzBrveBMjrLD

ACUTE TOXICITY TO FISH TEST SUBSTANCE Identity:Perfluorooctanoiaccid,ammonium salt;may alsobe referredto as PFOA ammonium saltA,mmonium perfluorooctanoateF,C116, FC-126, FC-169, or FC-143. (Octanoicacid, pentadecafluoro-a,mmonium salt,CAS # 3825-26-1) Remarks: 3M productionlotnumber 390. The testsample isFC-126, a whitepowdery solid.It'psuritywas not sufficientclhyaracterizedt,hough currentinformationindicatesitisa mixtureof78 - 93% testsubstance and 7 -22% C5, C6, and C7 perfluoroanalogue compounds. METHOD: Method: Not stated Type: Staticacute GLP: No Year completed: 1987 Species: Pimephalespromelas Supplier:Dale Fattigof Brady,Nebraska. Analyticalmonitoring:Temperature,pH and DO Exposure period:96-hours Statisticamlethods: Probitanalysis Test fishage: Not noted. Length and weight: Average length 3.5 cm Average weight 0.30 g Pretreatment: None. Test conditions: Dilutionwater: Carbon-filterewdellwater. Dilutionwater chemistry (initial): Temperature: 21 OC Dissolved Oxygen: 9.3 ppm pH: 7.9 Stock and testsolutionpreparation:Test solutionswere createdby directweightsaddition. Concentrations dosing rate:Once Stabilityof the testchemical solutions:Not noted Exposure vessels: 4 litePryrex(gg)lassbeakers containing3 litertsest solution. Number of replicates:two Number offishper replicate:6 Loading rate:0.60 g/L Number of concentrations:fiveplusa blankcontrol Water chemistryduringthestudy: Dissolvedoxygenrange(24- 96 hours): 6.6- 7.4mg/L(contreoxlposure) 5.6- 6.6 mg/L (320 mg/L exposure)* pH range (24 - 96 hours): 7.7- 7.9 (controelxposure) 7.7- 8.0 (320 mg/L exposure)* 320 mg/L testgroup (thirhdighestconcentrationd)ata given because totalmortalitoyccurredinthe two highertest concentrationsby 24 hours. Test temperature (24 - 96 hours): 200C RESULTS Nominal concentrations: Bk control,100, 180,320, 560, and 1000 mg/L Element value: 96-hourLCso = 301 (244 - 370) mg/L Element valuebased on nominal concentrations CONCLUSIONS The testsample 96-hour LC50 forfatheadminnow was determined to be 300 mg/L witha 95% Confidence Intervaolf240 to370 mg/L. Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St.Paul,Minnesota,55133 DATA QUALIT\( ReliabilitKyl:imischranking2. Testingmeets the criterifaorquality testing.However, sample puritywas not properlycharacterizedand it lacksanalyticaclonfirmationoftestsubstance concentrations. REFERENCES This studywas conducted by the 3M Company, Environmental Laboratory,St.Paul,MN, Lab Request Number E1282-1, completed on May 1,1987. OTHER Lastchanged: 5/24/00 ENVIRONMENTAL LABORATORY FISH TOXICITY DATA SHEET DEFINITIVE (FULL-SCALE TEST) LR-,F-l@Z fi3Z'--, TYPE TEST AVG. FISH TEST-qiowr, r4,-.@m CAATERIAL ORGAN ISMPa4V,41-1 LI,4'iitiOlSlO)URCE WT.-Arl@'--g5r(a)ms AVG. SIZE-Z&.,,@' cm DISEASE TREATMENT d6@h@) TESTED-t--li- 14113 nA TE REC'D NO. '6RGANISMS" 'PER DOSE LOT-@@L9t9 ;@-/l IF@-I BATCH NO..ILV OADING FACTOR/),/,!)Ig)/1 TEST SOLUTION VOLUME.,A@_LITERS/ALL-GLASS TANK TIME STARTED-tL.(Y).Rlm DATE STARTED-- TANK DIMENSIONS: 4 LITER PYREX@GLASS BEAKER DATE C014PLETE DILUP-ON-WATER INVESTIG CARBO -FILTERED WEI I WATER (TEMP Z)l OC. i D.O..qa,'5 ppm. pH Y, C,,,(95% CONFIDENCE LIMITS)-2-P(\-(P@l 2225@@@cqllAwi@ L-C-soC&uk'WM --6016@94 -,3'10) o(A /L-..) Tank Initial Total 24-Hours No. Conc. Amount Total No, PH D.0 mg/l Added Dead PPM 48 Hou s 72 Hou s 96 Hourr, Total No pH D.O. Total No. pH[pDD.O. Total tlo.'pH[,D.O. Dead PPM Dead PPM Deai PPM CONTRO 7,4 n nn 22 L/.] 0 v I -e n - 2L2&10 Ipn KZ C) C) Y9 /10p, r) 4 (Pz, 0 41 lry@ Test Temp. OC Initials & Date COt4.iENTS:.2@-Z'i 64-@c 1,A .!1.!2 .P<4 tA.1a-+,oAvi )Lt'r44-0e1-i@rlt- SFewi@'Ct*EtTS (OTHER THAN MORTALITY) Tank No. Symptoms o-rReactions 24-Hr. E SE I =raja[ Lc(7 @oN ry,&4I/QJ itoIyO - 14 1 v- A EC 50 Total Num er Affected 48-Hr. 72-Hr. 96-Hr. ENVIRONMENTAL LABORATORY FISH TOXICITY DATA SHEET DEFINITIVE (FULL-SCALE TEST) LR dE-12ic32t--l TYPE 1*EST- TEST ORGAN ISM k4i L4WIZ AlrERIAL,_TES SOURCE,-,-. AVG. WT.i),.W) grams AVG. SIZE @CM NO. FISH DISEASE TREATMENT.-A(,@g/-Ir I - .&',-DATE REC'D- ij If ORGANISMS PER DOSE..@OADING LOI".-@() /9"/ .1 ---r- BATCH NO -@Z@ FACTORV,IM 9/1 TEST SOLUTION VOLUME.6';J-LITERS/ALL-GLASS TANK TANK DIMENSIONS: 4 LITER PYREX@GLASS TIME STARTE D.1 Oapok DATE STARTE D- A-@- ?, 1-1-A" DATE C014PLETEi)- DILUT,Y&.UATER CKRBON-FILTERED WFII WATF FTEMP Zil - OC., D.O.-Cl.-r@PPM, PH- I.C INVESTIGA 50(95% CONFIDENCE LIMITS)-@5-V q(pw,ke5b tg" BEAKER LZ13*i4 Tank Initial Total No. Conc. Amount, mg/l Added 24 Hours Total No,'pHID.O. Dead PPM 48 Hou-s Total No. pH D.O. Dead PPM CONTRO V,C) 1/1)0M- 0 /1,0 9 OlZtz-Y/, o ARO 72 tal No Dead ours 96 Hours pH D.O. Total No. pH D.O. PPM Dead PPM '*7 0 (-)n ()(Y C) Test Temp. Oc Initials & Date COMMEfils:* k=ii iA IA 5ftg'PARi*fECTS ;I T0aann ',TNNo.@ S m to" Symptoms (OTHER THAN or Reactions MORTALITY) 24-Hr. CONTkROL Vi iim tc>-i,4 ,Nq4pv- -T t EC 50 Total Num er Affected 48-Hr. 72-Hr. 9 6-Hr.