Document k6nrznbb7a40Vj9q7roznekkD

ACUTE TOXICITY TO AQUATIC INVERTEBRATES (DAPHNIA MAGNA) TEST SUBSTANCE Identity:Mixturecontainingperfluorooctanesulfonatweh,ich may also be referredto as PFOS, FC-95, or as a component of FC-203CF-X or FC-203CF. (1-Octanesulfoniaccid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was 101. The testsample isFC203CF. Currentinformationindicatesitisa mixtureof 1% PFOS, 20% diethyleneglycolmonobutyl ether,70.2% water,2.75% Acrylicfoamer, 3% sodium octylsulfate,1% tdethanolamine,2% sulfonatedalkylethersalts,and 0.05% tolyltriazole. The followingsummary applies to a mixture with incompletely characterized concentrations of impurities.Data may not accurately reflecttoxicityof the fluorochemical component of the testsample. METHOD: Method: OECD Guideline202 Type: Acute static GLP: No Year completed: 1991 Species: Daphnia magna Supplier: In-houseculturesmaintainedatEnviroSystemsDivisioR,esource Analysts,Inc.,Hampton, NH. Analyticalmonitoring: Temperature, pH, conductivitya,nd dissolved oxygen. Exposure period: 48-hours Test organism age: <24 hours old Statisticamlethods: No calculationpserformed,based on study results. Test conditions: Dilutionwater: Filterednaturalwellwater Dilutionwater chemistry: Conductivity: 840 pmhos/cm pH: 7.3 Lighting:Cool-whitefluorescentlightsat 20 pEin/seC/M2. A daily photoperiodof 16 hours lightand 8 hours dark was maintainedthroughoutthe testingperiod. Stock and testsolutionpreparation: A primary1000 mg/L stock solution was prepared and added directltyocreatetestsolutions. Exposure vessels: 250 mL beakers containing200 mL exposure solutionat a water depth ofapproximately6 cm. Number of replicates:4 Number of daphnids per replicate:5 Numberofconcentrationfsi:vpelusa blanckontrol Waterchemistrdyuringthestudy: pH range(0-48hours): 7.3- 8.2(contreoxlposure) 7.5- 8.3(1000mg/L exposure) Temperature range (0-48hours): 19.1- 19.6OC (controelxposure) 19.1- 19.6-C (1000mg/L exposure) Conductivityrange (0-48hours): 840 - 860 pmhos/cm (controelxposure) 860 - 870 pmhos/cm (1000 mg/L exposure) Dissolved oxygen range (0-48hours): 8.3- 9.2mg/L (controelxposure) 8.3- 9.2mg/L (1000mg/L exposure) RESULTS Nominal concentrations:Blankcontrol1,50,250,400, 600,and 1000 mg/L. Elementvalues: 48-hourEC50=>1000mg/L Element valuesbased on nominalconcentrations. Remarks: Testingwas conductedon themixtureas describedintheTest Substance Remarks fieldT.he valuesreportedapplytothatmixtureand not the fluorochemicaplroportionalone. CONCLUSIONS The testsubstance48-hourEC50was determinedto be >1000 mg/L. No mortalitoyr sublethaleffectswere observed. Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking= 2.Thisstudymeets the criterifaorquality testing.However, thesample puritywas notpropedycharacterizeadnd the studylacksanalyticaclonfirmatioonftheamount offluorochemicaplroportion inthe solution. REFERENCES Thistestwas conductedby EnviroSystemsDivisionR,esource Analysts, Incorporatedo,f Hampton, NH atthe requestofthe 3M Company, St.Paul, MN, Lab Request number J1884-3,1991. I OTHER Last changed: 6/28/00. Study Title Static imte Toxicity of IrC-203CF to the DapMid, DitphniaJB&gna Authors Timothy J. Ward Robert L. Boori Study Coaplated July, 1991,@ ina Laboratorv'ZnviroSystess:Dlvlslozk@,. @t@t. Resource maysts i,.131corporat*d oneiL:ataietti load, "Z"PtOn',,'Ngw,IsiPshirs:@"@03942 The acute toxicity of fC-203CY to the daphnid,D&PMI& sagnrThe test was conducted for 3H is describedin this final report. Company for 48 hours during June 1.2 to 14. 1991, at the Znviro$ystess in Rampton. Now Hampshire. It was Division at Resourcemalysts, Inc conducted by Jeanne Magazu. Peter Umalskil illon Stanford,Robert Doori, ud Timothy Vard. performed under static conditionswith five conceThnetrattieosntsofwastest substance and a dilutionwater controlat a temperature of 20 1 1*C. The dilutionwater was tilterednaturalwell water collected at Rampton,Now laspabire. Aerationwas not employed to maintain dissolvedoxygenconcentrationsabov* an acceptablelevel. Nominal concentrationsat PC-203C? were-.0 mg/L (control).150 mg/L, 250 ag/L, 400 mg/L.600 no/L. and 1,000 mg/L. Nominalconcentrations were used for all calculations. Dapbnids used in the test were producedfrom an in-houseculture and were less than 24 hours old at the start of the test. ktter 48 boars at exposure the control dapbuids had an averagewet weight (blotted dry) at 0.0008 g, resultingin a loadingrate at 0.02 g/L. All daphnidswere in good conditionat the beginningof the study. The test substance was not toxic to daphaidsat tested Zzposure of dapbuids to the test substance resulted concentrations. ICSO greater than 1,000 mg/L rC-203CT. No sublethal in a 49 effectwas hour observed in any test vessel during the test. Page 2 of.1 R-- Pro*," I ii.TABLZ OIF C@l@s SZCTION: 1. sumary 3 Table of COntOnts .'Indexof Tables and rigures IV. introduction V. methods and Katerials vio Results References Appendix-1. Water Quality Data from TaxicitY Test 12 4e . z iii. IN= Of TAB= An RICUM Table 1. chanical characterization of a representative saapl* of natural well water used as dilution water for toxicitytest Table 2. Survivaldata from toxicitytest Table 3. Median effectiveconcentrations(EC50s) from toxicitytest Table %.I. Conductivity,pH. tesperature.and dissolved oxygen concentrationasasured during toxicity test P&GZ 6 9 10 13 Mawr-, study was sponsoreiby 3B C"My, St. Paul, Hinuesata. The This was.to determinethe acute toxicity of FC-203Cf to objective of the study the dapbnid,a freshwaterinvertebrate. The report contains sectionsthat describe the notbods and materialsemployed in the study, and the results of the investigation. The report also contains an appendix that presents the water qualitydata collectedduring tb* test. T. NEMDS MM NATIRIMA TEST SUBSTANCE: FC-203CT (EnviraSystans Sample guber 29379) was delivered to EnviroSystess an May 23, 1991. It was contained in a 40 oz plastic bottle that was labelled with the following information:"JIS$4-3, TC-203Cr". rC-203CF. (an orange-brown liquid) was supplied by 31 Company. 935 Bush Avenue, St. Paul, Hinnesota. Prior to use the test material was stored in the dark at room tea"rature. A reserve mople (approximately1.0 gran) will be retained at ZnviroSysteasfor a minis= of 10 years. DILUTION VKTU: Water used for acclimationof test arganisas and for all toxicity testing was collected .from wells at ZaviraSystem in Hampton, Now Hampshire. Water was stored in 500-vallon polyethylene tanks where it was aerated. R*sults of chemical analysis of a representative sample of water are presented in Table 1., TEST (mrmasn. a diii@a4 isploya as 6,r,ta,nizawse,re ran a,,single susoeuar.c.e-i.na ntdhe wteerset -identifiedusifnrgomananapipnr-obpmrsieatceutlatxuornoomaincd kweeyr.e lDeaspsbutihdasn s@,,old at@.:thiistirotf the test..:'Cmtioldavwdswere weighed a ".Conclusion.: tbi@"@,-toideity,test. :,,Priorto tiitini,-dapbnids.w.ere itained: in,loo%",dilutionwater under static conditiiiis-:i4n17liter gLass-@.,, ars. During ac6lization.dapwds were not,treated'. and ore ir'iiot., usaire.-a,ickness; .injuries and abam 'iillLtii@@tkt During the acciinit:iokper -boom, lprior.;vu no of,(-t@he,: test;-@.-t4 test jiftiatiatue"ta"spera iire was@l@.S DapWd*.@. @ort@ 'Yeast trout chm, i-,"ftreshiater-@-:,-Saillgon or twice daily.btfortehe tea Table 1. Chemical characterizatioonf a representativesample of naturalwell waterused as dilationwater for toxicitytest Parameter PR Conductivity organochlorine pesticides Organopbospborus pesticides Polychlorinated bipbonyls Unit of measurement Reporting Limit PH units unhos/cm - ug/L 0.5 VO/L ug/L 1 Measured value 7.3 940 ND ND ND .Notes: 1. NDa not detected at or above the reporting limit. 2. pa, hardness, and conductivity were measured in dilution water collected froa the control test vessel prior to Introduction of organism and.puticide and PCB data is ...... collected during routine biannual water quality testing.", 4v. Page ARM TOXICM TZSTrno: A screening test with the testsubstancewas conductedduring Ray 29 to 31, 1991. Nominal concentrationsof test substance were 1.0 ag/L, 10 mg/L, 100 mg/L, and ITOOO ag/L. After 48 hours of exposure there was 20% survival at 1,000mg/L. and 80% survival or greater at 1.0 mg/L, 10 ag/L. and 100 mg/L. Surviving daphaids expmed to 100 mg/L at 49 hours and 1,000 ag/L at 24 to 48 hours were imabilized. The definitivetoxicity test was performedduring June 12 to 14, 1991. The test was conducted at a target temperatureof 20 1 IOC with five concentrationsof test substance and a dilutionwatercontrol. 1 1,000 mg/L stock solution was formulated without the use of a solvent. Nominal concentrationsof the test materialwere: 0 mg/L (control),150 mg/L, 250 mg/L, 400 ag/L. 600 mg/L, and 1,000 ag/L. Twenty daphnids were randomly and equally distributed among four replicates of each treatment. The test was performed in 250 al glass beakers that contained 200 al of test solution (water depth was approximately 6 ca). Test vessels were randomlyarrangedin an incubator during the 48 hour test (a random numbers tablewas used to select the location of each vessel). A 16 hour light and 8 hour dark pbotoperiod was automatically maintained with cool-whit*fluorescent lights that provideda light intensityof 20 ugs-'s-2.Aeration was not required to maintain dissolvedoxygenconcentrationasbove acceptablelevels. Daphnidswere not fed duringthe test. The number of surviving organism and the occurrence of iambilization (inabilityto swin within 15 seconds after gentle agitation)o,r other sublethaleffects (lossof equilibrium,erratic swimming, Its* of reflex, excitability,discolorationo,r changein behavior) were determinevdisuallyand recordedinitiallyand after24 and 49. hours. Dissolvedoxygen(TSINodel57 meter;instrumentnumber (B*ckmm nodal VU 12 motor;instrusentnumberPRL-51). conductivity(Cole Pamer number .1431-60,','instrnuusmebnetrPRL-50), and temperature(AM mercury thermometer.;@t.hermometenrumber2265) were measured and recorded dailyin each test chamberthat contained live animals.,: STAN STicn UMODS: 4;' 'Results of the toxicity toot,.could:not be interpreted by standard.@@statistictaelchniques(Stepbii;1983)because 50% survivalor greater occurredat all testedconcentrations.,. Page 7 of 14, RZMTI 203Cywife initiallyclear all'& III test vessels containing rc- and water qwaity data 3iolcqical remained clear throughoutthe t"t* prosesktodin Table 2 and generated by tb* acute toz'ct' test aret suvival occurredin tbO Xppemdil JL,r"pectivtll- out hundred PercOu Control dapbnids bad an average lietweight (blotted cdornyt)roolf e0x.po0s0u0r8e_q at the 0", .of the test Loading rate during the toxicitytest was approximatelyo.02 g/L. The . test substance --was not toxic to dapbnids at- tested TM 24 .and'..418 hour ZCSOs for dalobnids exposed to concentrations - sented in "-.Table3. gzposure of dapbaids to rC-203CY TC-203Cr are PrO go sublethal LCS() greater than1,000mg/L- resulted in a 48 'hour effectwas observedin any test,vessalduringthe tgst- Ar , -n 'Pace 3 Table 2. survivaldate from toxicity test Nominal Concentration (ag/16) rep. Number Alive 0 hr 24 hr 48 hr Number kffected Ohr 24hr 48br 0 (control) 1 2 3 4 150 2 3 4 250 1 2 3 4 400 1 2 3 4 600 3 1 1000 2 5 5 5 5 5 5 5. 5 5 5 .5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 s 5 5 5 5 5 5 5 5 5 5 5 5 5 5 s 5 5 S 5 5 55 5 S 5- 5 5 5.1 5 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 .0 0 0 .0 0 0 0 0 0 0 0 0 @i@ u0 0 0 0 0 Note. IS,'nk,rt @.lmwbili.ze.d(,u.n-able,t-o swim witbin 15 sconds"afte T@* Table3. Kadineffectivceoncentratio(nsscsoof)romtoxicittyest szp"ure period 95 pereent:@ zcso confidencelinits calculation nothod 24 bourn )1,000mg/L 40 hours )1,000mg/L IA;A k"r t 14 Page 10 o Vil. alwomm OZCD. igs4. cuidelinefsor Testingof ChosiC&12.Section.2I:ffectsan Biotic systems. Nothod 202,Daphnidop.. Imte ImobilizationTest and..,. ReproductioTnest. AdoptedApril4, 1984. '14 L,,F Appendix A. WATN OUALITY DXTA nOR TOXICITY TICST ly Table A.I..Conductivity tmerature -and dissolved oxygen concentration seastred during toxicitytest Nominil concentration (ag/L) Rep. -ComAnctivity (mbo/ce) 0 24 48 hr hr br 0 (control) 1 840 2 $40 3 $40 4 840 150 1 850 2 $50 3 $50 41 sso 250 1 $so 2 $50 3 $50 4 $50 400'.. 1 $so 21-- - $50 3 $50 4' $50 600 -k;.4' .' - 0 850 950 000 W@8601@@; lwi, $60 $66. $60 850 $so $60 $40 $40 $40 $40 $50 $so $so 850 860 $60 $60 860 $70 $70 870 70 $70@ 870 87 o 970'-' 860 $60 860 $60 $30 $30 840 $40 $40 $50 $50 $50 $70 870 $70 $70 $70 $70 $70 $70- 0 24 48 hr hr 7.3 7.3 7.3 7.3 7.3 7.3 7.3 7.3 7.4 7.4 7.4 7.4 7.5 7.5 7.5 7.5 7.5 . 4 ?.S 7.8 7.9 7.9 7.9 8.0 8.0 8.0 8.0 8.1 8.1 9.1 8.1 8.1 S. 8.1 8.1 8 8.11. 8.0 8.1 9.2 1.2 6.2 8.2 8.2 8.2 9.2 $.2 8.2 8.3 8.3 8.3 @'t 3 @3@ .7 75 7 5--l. I;o 7 Table &.I. Continued. Nominal concentration (SO/L) Rep. 0 (control) 1 2 3 4 ISO 1 2 3 4 250 1 2 3 4 400 1 2 3 4 600 1 2 3 4 10 1 2 3 Temperature (OC) 0 24 48 hr hr hr Dissolved Oxnen (ag/b) 0 24 48 br br kv 19.5 19.2 19.4 8.4 9.2 8.7 19.6 19.1 19.5 8.3 9.0 9.7 19.5 19.1 19.5 8.3 9.0 9.7 19.5 19.3 19.5 8.3 9.0 8.7 19.3 19.2 19.3 7.9 9.1 8.8 19.6 19.1 19.5 7.9 9.1 8.8 19.5 19.0 19.4 7.9 9.1 8.8 19.6 19.2 19.3 7.9 9.1 8.3 19.6 19.0, 19.3 8.0 9.1 8.9 19.5 19.1 19.2 8.1 9.1 8.8 19.5 19.2 19.3 8.1 9.2 8.8 19.5 19.1' 19.1 8.1 9.2 8.8 19.4 19.0 19.2 8.2 9.2 8.8 19.8 19.1 19.4 8.3 9.2 $.$ 19.S 19.2 19.3 8.2 9.1 9.6 19.5 19.2 @19.5 8.2 9.2 19.6 19*2'ti@-@t,,'1*9 3 8.3 9.2 9.8 19.7 1912', " 19:6 9.3 9.2 9.8 1199.66 19.1 19.2 8.3 9.2 .8.8 19 1 8.3 9.2 19.6 1-.1 19 .5 8.3 9.2 19.5 19'.' 19 .3 8.3 9.2 19.5 -19.2 8.3 92 19.6 92 19 3 8.3 9.2 S. T'T