Document jyxQbjN4k9Jawb04DJgzom5xO
MICROBICS MICROTOXS TOXICITY TEST
TEST SUBSTANCE
Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may alsobe referredto as PFOS, FC-95, or as a component of FC-603F. (1Octanesulfonicacid)(CAS # 2795-39-3).
Remarks: The 3M producton lotnumber was 501. The testsample isFC603F. Currentinformatioinndicatesitisa mixtureof0.5% PFOS, 7% diethyleneglycolbutylether,82.95% water,2% acrylifcoamer,4% Alkylether sulfate1,.45% Xanthan gum, 1.4% calciumacetate,0.07% N-(3Chloroallyl)hexaminiucmhloride0,.5% tdethanolamine,0.05% sodium hydroxide,0.03% cis-1-(3-Chioroallyl)-3,5,7-td1a-zaaz-oniaadamantane chloridea,nd 0.05% tolytriazole.
The followingsummary applies to a mixture with incompletely characterizedconcentrations of impurities.Data may not accurately reflectthe toxicityof the fluorochemical component of the testsample.
METHOD:
Method: MicrobicsMicrotoxO "BASIC" Procedure Test type: Staticacute GLP: No Year Completed: 1994 Species: Photobacteriumphosphoreum Analyticalmonitoring:pH and osmoticcondition. Exposure period: 30-minutes Statisticamlethods: EC50 valuescalculatebdy statisticlailnearegression program providedforMicrotoxV Test organism source: Microbics'Reagent, LotAM025 Test conditions:
Diluent: MilliporMeilli-QTwmater Test temperature: Not noted. Stock and testsolutionpreparation: A 2000 mg/L primarystock solutonwas prepared by dissolvin2g00 mg testsubstance in100 mL MilliporMeilli-O@wmater. The pH ofthestocksolutionwas 6.7.An osmoticadjustmentwas made using200 mg NaCi dissolvedin10 mL stocksolution.Appearance oftestsoluflonwsas noted as "clearand colodess".Alltestsolutionswere made by proportionadlilutions.
Exposure vessels: 4 mL cuveftes. Number of replicates:two Number of concentrations: Four plusa blankcontrol Element basis: Percentlightloss
RESULTS Nominal concentrations:Blank control1,25,250, 500, and 1000 mg/L
Element value:
5-minuteECro = 480 (450 - 510) mg/L 15-minuteEC5o = 390 (370 - 420) mg/L 30-minuteEC5o = 340 (320 - 350) mg/L
Element valuesbased on nominal concentrations.
Remarks: Testingwas conducted on a mixtureas describedinthe Test Substance Remarks field.The values reportedapplytothatmixtureand not the fluorochemicalproportionalone.
CONCLUSIONS
The FC-603F 30-minute EC50 forPhotobacterium phosphoreum was determined tobe 340 mg/L witha 95% confidenceintervaolf 320 to350 mg/L.
Submitter: 3M Company, Environmental Laboratory,P.O. Box 33331, St. Paul,MN, 55133
DATA QUALITY
ReliabilityK:limischranking= 2.This studymeets the criterifaorquality testing.However, sample puritywas notpropedy characterizedand the study lacksanalyticaclonfirmationofthe amount offluorochemicalproportioninthe solution.
REFERENCES
Thisstudywas conducted by the 3M Company, EnvironmentalLaboratory, St.Paul,MN, Lab Request number Ml 655, 1994.
OTHER
Last changed: 6/26/00.
3M ENVIRONMENTAL LABORATORY
Page I of
Microtox Test Data Summary
LF;LM1655 Date:5/20/94 Analyst:JM2
SAMPLE: FC-603F (Lot#501)
Sample Conc.
% ReducfionInBioluminescence
(mg/L)
5 min
15 min
30 min
125
is
24
25
250
35
39
43
500
50
55
59
---1000--l---68 --- ---73---- ...ZZ...
EC50
480 mg/L 390 mgtl 340 mg/L
(95% C.I.) (450-510) (370-420) (320-350)
@m 10620 1 -0 -owo
Sample Descriptlon
Lab Request No.
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